CN109432006A - A kind of coccidial vaccine is sprayed immune adjuvant and its application - Google Patents
A kind of coccidial vaccine is sprayed immune adjuvant and its application Download PDFInfo
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- 239000002671 adjuvant Substances 0.000 title claims abstract description 30
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- 206010012735 Diarrhoea Diseases 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/12—Aerosols; Foams
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/002—Protozoa antigens
- A61K39/012—Coccidia antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/39—Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
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- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/54—Medicinal preparations containing antigens or antibodies characterised by the route of administration
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/55—Medicinal preparations containing antigens or antibodies characterised by the host/recipient, e.g. newborn with maternal antibodies
- A61K2039/552—Veterinary vaccine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55511—Organic adjuvants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55511—Organic adjuvants
- A61K2039/55583—Polysaccharides
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- General Chemical & Material Sciences (AREA)
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Abstract
Immune adjuvant is sprayed the invention discloses a kind of coccidial vaccine and its application, the adjuvant include the ingredient of following parts by weight: 10~34 parts of guar gum, 2~34 parts of chitosan oligosaccharide, drying up 1.5~10 parts of powder, 1.5~7 parts of edible pigment, 15~85 parts of DEXTROSE ANHYDROUS；The adjuvant can rapidly dissolve in vaccine diluent at normal temperature, coccidial vaccine is set to be uniformly dispersed, and the long period it can keep moisture, it is easy to use, it is mixed in coccidial vaccine dilution and is sprayed directly on chicken by being sprayed equipment, suitable for intensive chicken farm and large-scale hatchery, establish chick quickly immune.
Description
Technical field
The present invention relates to biological immune technical field, in particular to a kind of coccidial vaccine is sprayed immune adjuvant and its application.
Background technique
Chicken coccidiasis is a kind of extremely serious global parasitic disease of harm, by push up multiple door Sporozoa Eimeria one
Kind or a variety of Eimerias parasitize in chicken intestinal mucosal epithelium, and diarrhea, bloody stool, growth retardation etc. is caused to influence chicken production
Development is even dead, all causes huge economic losses to poultry husbandry every year.Currently, coccidiosis is essentially relying on feed or drinking-water
Middle addition coccidiostat.But since coccidia drug resistance problems are more and more serious, and list coccidial vaccine safety and effectively
Property gradually obtain market approve, using coccidial vaccine prevention chicken coccidiasis become new trend.Coccidial vaccine immunization method includes
It drinks water, mix the methods of (spray) material, drip, spraying, the immunization method mainly promoted the use of at present is that drinking-water is immune, by coccidial vaccine
After mixing with suspending agent, it drinks and is immunized for 3 to 7 age in days chickens in water kettle.Although drinking-water it is immune compared with spice,
Drip is the methods of immune easy to operate, but its application range has some limitations, and intensive chicken farm is not suitable for
It is immune with drinking-water.Immune traditional spray is to be atomized to spray by spraying apparatus after diluting vaccine, and chick is through respiratory tract or freedom
It is immunized after pecking at.Spraying immune is sprayed after vaccine is diluted with water at present, and the drop of ejection is smaller, is attached to
Moisture on feather is easy evaporation, causes part chick that can not obtain effectively immune.
As it can be seen that the prior art could be improved and improve.
Summary of the invention
Place in view of above-mentioned deficiencies of the prior art, the purpose of the present invention is to provide a kind of coccidial vaccines to be sprayed immune use
Adjuvant and its application, it is intended to solve the problems, such as it is above-mentioned point out it is in the prior art at least one.
In order to achieve the above object, this invention takes following technical schemes:
A kind of immune adjuvant of coccidial vaccine spray drop, the ingredient including following parts by weight:
10~34 parts of guar gum,
2~34 parts of chitosan oligosaccharide,
1.5~10 parts of powder of drying,
1.5~7 parts of edible pigment,
15~85 parts of DEXTROSE ANHYDROUS.
The coccidial vaccine is sprayed in immune adjuvant, the ingredient including following parts by weight:
12~24 parts of guar gum,
10~34 parts of chitosan oligosaccharide,
1.5~10 parts of powder of drying,
1.5~7 parts of edible pigment,
15~85 parts of DEXTROSE ANHYDROUS.
The coccidial vaccine is sprayed in immune adjuvant, and the edible pigment includes carmine, indigo, brilliant blue, green grass
One of green, amaranth, lemon yellow, tartrazines, sunset yellow.
A kind of coccidial vaccine is sprayed the application of immune adjuvant, comprising: coccidial vaccine is diluted with water, pours into while stirring
Above-mentioned adjuvant, stirs evenly, and is fitted into and is sprayed in equipment, directly sprays to chicken.
The utility model has the advantages that
The present invention provides a kind of coccidial vaccines to be sprayed immune adjuvant and its application, and the adjuvant is easy to use, at normal temperature
It can rapidly dissolve in vaccine diluent, coccidial vaccine made to be uniformly dispersed, and the long period can keep moisture, be suitable for intensive
Change in chicken farm and large-scale hatchery, establishes chick quickly immune.
Specific embodiment
The present invention provides a kind of coccidial vaccine and is sprayed immune adjuvant and its application, to make the purpose of the present invention, technical side
Case and effect are clearer, clear, and the present invention is described in more detail by the following examples.It should be appreciated that described herein
Specific examples are only used to explain the present invention, is not intended to limit the present invention.
The present invention provides a kind of coccidial vaccine spray drop immune adjuvant, the ingredient including following parts by weight:
10~34 parts of guar gum,
2~34 parts of chitosan oligosaccharide,
1.5~10 parts of powder of drying,
1.5~7 parts of edible pigment,
15~85 parts of DEXTROSE ANHYDROUS.
Guar gum is a kind of High Purity polysaccharide extracted from the cluster bean planted extensively, using poly- mannose as molecule master
Chain.Guar gum is water-soluble best one of natural polymer, can be dissolved in cold water and form viscous liquid.Guar gum is modern food
One of widest food dressing of industrial application has the function of thickening, water conservation, stablizes and bond suspension.By coccidial vaccine and Guar
Glue solution mixing is able to maintain the evenly dispersed suspension of coccidian oocyst, and keeps moisture, and then guarantees that coccidian oocyst vigor is not lost.
Chitosan oligosaccharide is a kind of widow of degree of polymerization for being degraded by chitosan through special biological enzyme technology between 2~20
Sugar product has the advantages such as molecular weight is low, good water solubility, be easily absorbed, bioactivity height.Since guar gum is natural polymer
Glue, easily by enzyme and bacterial degradation, chitosan oligosaccharide has broad-spectrum antibacterial activity and to person poultry harmless, and chitosan oligosaccharide is added in the adjuvant can
Aqueous guar glue solution is kept to be not easy to be gone bad by bacterial degradation, meanwhile, addition can improve animal intestines and stomach to chitosan oligosaccharide in right amount in feed
Road microbial environment, strengthen immunity promote growth of animal.
Edible pigment is used as a kind of color developing agent in the adjuvant, and chick is attracted to peck at.
Drying up powder is a kind of desiccant, for preventing chitosan oligosaccharide and the DEXTROSE ANHYDROUS moisture absorption during preservation rotten.
DEXTROSE ANHYDROUS can be quickly dissolved into water as a kind of sweetener, also can be used as dispersing aid, increase dispersion medium
Viscosity, and the surface for being adsorbed on suspended particles increases its hydrophily, is conducive to the sinking speed and coalescence that reduce particle, increases
Add the stability of suspension.
For the present invention by the way that above-mentioned five kinds of ingredients are mixed the coccidial vaccine adjuvant according to a certain percentage, which can
It is quickly dissolved in cold water, cooperation is sprayed equipment and uses, and the drop of ejection is attached to chick feather and does not drip, and can save moisture
It does not dry, guarantees the vigor of coccidian oocyst, the spray drop that can be applied to coccidial vaccine is immune.
Preferably, the coccidial vaccine is sprayed immune adjuvant, the ingredient including following parts by weight:
12~24 parts of guar gum,
10~34 parts of chitosan oligosaccharide,
1.5~10 parts of powder of drying,
1.5~7 parts of edible pigment,
15~85 parts of DEXTROSE ANHYDROUS.
Specifically, the edible pigment include green carmine, indigo, brilliant blue, green grass, amaranth, lemon yellow, tartrazines,
One of sunset yellow.Above-mentioned pigment is nontoxic, will not generate adverse reaction after chicken feed, and bright-colored, be easy
Cause chicken note that be easy to be searched for food in turn.
A kind of coccidial vaccine is sprayed the application of immune adjuvant, comprising: coccidial vaccine is diluted with water, pours into while stirring
Above-mentioned adjuvant, stirs evenly, and obtains the evenly dispersed viscous fluid of egg capsule, is fitted into and is sprayed in equipment, directly sprays to chicken, described
Vaccine diluent is attached on chick feather and does not drip, and moisture is kept to be not easy drying, guarantees the vigor of coccidian oocyst, for young bird
Chicken is immunized after freely pecking at.The spray drop immunization method without intervening the feeding of chicken, reduce stress with it is artificial clear
Water kettle is washed, is suitable for intensive chicken farm and large-scale hatchery, 1 Japanese instar chickling can be quickly immunized after shell out.
Applicant demonstrates through a large number of experiments to be significantly improved using immune effect after the adjuvant, selects tool below
5 embodiments of body and without the coccidial vaccine using the adjuvant it is immune as a comparison case, the application is made further
Explanation.
Embodiment 1
Guar gum 2.5g, chitosan oligosaccharide 0.5g are taken, powder 0.375g, edible pigment 0.375g, DEXTROSE ANHYDROUS 21.25g, mixing are dried up
Investment has been loaded in the beaker of 500mL coccidial vaccine dilution after uniformly, stirs evenly, obtains the solution 1 with stickiness.
Embodiment 2
Guar gum 4g, chitosan oligosaccharide 3.4g are taken, dries up powder 0.6g, edible pigment 1g, DEXTROSE ANHYDROUS 11g are put into after mixing
It in the beaker for having been loaded with 500mL coccidial vaccine dilution, stirs evenly, obtains the solution 2 with stickiness.
Embodiment 3
Guar gum 3.6g, chitosan oligosaccharide 4.5g are taken, powder 0.75g, edible pigment 0.9g, DEXTROSE ANHYDROUS 5.25g are dried up, is uniformly mixed
Investment has been loaded in the beaker of 500mL coccidial vaccine dilution afterwards, stirs evenly, obtains the solution 3 with stickiness.
Embodiment 4
Guar gum 3g, chitosan oligosaccharide 1g are taken, dries up powder 1g, edible pigment 0.4g, DEXTROSE ANHYDROUS 4.6g are put into after mixing
It in beaker equipped with 500mL coccidial vaccine dilution, stirs evenly, obtains the solution 4 with stickiness.
Embodiment 5
Guar gum 1.87g, chitosan oligosaccharide 1.87g are taken, powder 0.55g, edible pigment 0.385g, DEXTROSE ANHYDROUS 0.825g are dried up, is mixed
Investment has been loaded in the beaker of 500mL coccidial vaccine dilution after closing uniformly, stirs evenly, obtains the solution 5 with stickiness.
Comparative example 1
It takes 1g edible pigment to put into the beaker for having been loaded with 500mL coccidial vaccine dilution, stirs evenly, obtain solution 6.
Solution obtained in above-described embodiment 1~5 and comparative example 1 is sprayed on 1 Japanese instar chickling using equipment is sprayed respectively, often
100 chick of group.After spray drop 30 minutes every group select 20 plumage chicken dissects at random, observe oral cavity, the esophagus, crop, gland of every chicken
Whether stomach has the color of edible pigment, if so, then proving that chick eats coccidial vaccine drop.The testing result of every group of chick
As shown in table 1.
Chick gastrointestinal tract dissect result in 1 Examples 1 to 5 of table and comparative example 1
| Group | Oral cavity | Esophagus | Crop | Glandular stomach | Recall rate |
| Solution 1 | 2/20 | 7/20 | 20/20 | 20/20 | 20/20 |
| Solution 2 | 4/20 | 10/20 | 20/20 | 20/20 | 20/20 |
| Solution 3 | 5/20 | 10/20 | 20/20 | 20/20 | 20/20 |
| Solution 4 | 3/20 | 6/20 | 20/20 | 20/20 | 20/20 |
| Solution 5 | 0/20 | 4/20 | 20/20 | 20/20 | 20/20 |
| Solution 6 | 0/20 | 2/20 | 9/20 | 9/20 | 9/20 |
Dissect in table 1 the result shows that, the 20 plumage chickens that the group that Examples 1 to 5 is added to the adjuvant is selected at random crop,
Glandular stomach has the color for observing edible pigment, illustrates that every plumage chicken has and pecks to vaccine drop, and only adds edible pigment
Vaccine diluent only has the color that 9 plumage chickens observe edible pigment in crop and glandular stomach, and test proves, after adding the adjuvant,
The vaccine diluent holding time after spray drop is longer, more conducively the feeding of chick, and then improves immune effect.
It is worth noting that the edible pigment in above-described embodiment 1~5 and comparative example 1 be selected from it is carmine, indigo, bright
It is blue, green grass is green, one of amaranth, lemon yellow, tartrazines, sunset yellow, the specific choice of above-mentioned several pigments is to test
As a result it does not impact, impartial prompt effect is generated to the feeding of chick.
It, can according to the technique and scheme of the present invention and its hair it is understood that for those of ordinary skills
Bright design is subject to equivalent substitution or change, and all these changes or replacement all should belong to the guarantor of appended claims of the invention
Protect range.
Claims (4)
1. a kind of coccidial vaccine is sprayed immune adjuvant, which is characterized in that the ingredient including following parts by weight:
10~34 parts of guar gum,
2~34 parts of chitosan oligosaccharide,
1.5~10 parts of powder of drying,
1.5~7 parts of edible pigment,
15~85 parts of DEXTROSE ANHYDROUS.
2. coccidial vaccine according to claim 1 is sprayed immune adjuvant, which is characterized in that including following parts by weight at
Point:
12~24 parts of guar gum,
10~34 parts of chitosan oligosaccharide,
1.5~10 parts of powder of drying,
1.5~7 parts of edible pigment,
15~85 parts of DEXTROSE ANHYDROUS.
3. coccidial vaccine according to claim 1 is sprayed immune adjuvant, which is characterized in that the edible pigment includes rouge
Rouge is red, indigo, brilliant blue, green grass are green, one of amaranth, lemon yellow, tartrazines, sunset yellow.
4. the application that a kind of coccidial vaccine as claimed in any one of claims 1 to 3 is sprayed immune adjuvant, which is characterized in that
Include: that coccidial vaccine is diluted with water, pour into above-mentioned adjuvant while stirring, stir evenly, is fitted into and is sprayed in equipment, directly to chicken
Sprinkling.
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| CN114146169A (en) * | 2021-12-28 | 2022-03-08 | 佛山市正典生物技术有限公司 | Carrier composition for chicken coccidian droplet injection immunization, use method and chicken coccidian droplet injection immunization composition |
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