CN109307541B - Method for measuring volume of lighting plate single-hole residual liquid - Google Patents
Method for measuring volume of lighting plate single-hole residual liquid Download PDFInfo
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- CN109307541B CN109307541B CN201811441555.4A CN201811441555A CN109307541B CN 109307541 B CN109307541 B CN 109307541B CN 201811441555 A CN201811441555 A CN 201811441555A CN 109307541 B CN109307541 B CN 109307541B
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- 239000007788 liquid Substances 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 17
- 239000000758 substrate Substances 0.000 claims abstract description 20
- 239000011248 coating agent Substances 0.000 claims abstract description 16
- 238000000576 coating method Methods 0.000 claims abstract description 16
- 102000004190 Enzymes Human genes 0.000 claims abstract description 12
- 108090000790 Enzymes Proteins 0.000 claims abstract description 12
- 238000005406 washing Methods 0.000 claims abstract description 12
- 238000004140 cleaning Methods 0.000 claims abstract description 11
- 238000002558 medical inspection Methods 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000003197 catalytic effect Effects 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
- G01F22/00—Methods or apparatus for measuring volume of fluids or fluent solid material, not otherwise provided for
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- Physics & Mathematics (AREA)
- Fluid Mechanics (AREA)
- General Physics & Mathematics (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a method for measuring the volume of residual liquid in a single hole of a light-emitting panel, which comprises the steps of firstly, respectively adding a light-emitting substrate into a coating hole of a blank light-emitting panel according to a1 mu l, a2 mu l and a3 mu l of each hole, and adding equal amount of enzyme, and establishing a standard curve after the light-emitting value of each hole is measured by a luminometer; replacing a plate washing machine washing liquid with a luminescent substrate, and cleaning the blank luminescent plate; adding equal amount of enzyme into the coating hole of the luminescent plate, and measuring the luminescent value of each hole by using a luminometer; and (4) bringing the light-emitting value of each hole into a standard curve in sequence to obtain the volume of residual liquid in each coated hole in the light-emitting plate. The method has the advantages that the method is simple, the volume of residual liquid in each coating hole of the light-emitting panel after being cleaned by the panel washer can be accurately measured, the volume of trace liquid below 1 mu l can be measured if necessary, the accuracy and reliability of the cleaning data of the light-emitting panel are ensured, and meanwhile, the reliable data are provided for medical inspection.
Description
Technical Field
The invention relates to a luminescent plate for in vitro diagnosis, in particular to a method for measuring the volume of single-hole residual liquid of the luminescent plate.
Background
The luminescent plate comprises a 6-48-hole cell culture plate, a 96-hole enzyme label plate and the like, is a reaction plate used in an in vitro diagnosis and detection experiment, and is cleaned by a luminescent plate cleaning device, namely a plate washer after the experiment is finished.
After the luminescent plate is cleaned by using the plate washer, a certain amount of lotion still remains in the luminescent plate coating hole, and the volume of residual liquid remaining in the coating hole is used as an index for judging the performance of the plate washer in the industry. The plate washer is generally matched with an enzyme-labeling instrument/a luminescence instrument for use, and is widely used for cleaning luminescent plates in hospitals, blood stations, epidemic prevention stations, reagent manufacturers and research rooms. Compared with the final luminous reading, the plate washing link is an unobtrusive link in the experimental process, does not generate the final analysis result, and is easy to ignore by people. However, their performance is also closely related to the end result. Especially, in a 96-hole plate washing machine, the flatness of the washing head directly influences the volume of residual liquid, while the plate washing is generally carried out for 5-6 times, the residual liquid effect is accumulated, and the precision result of the whole plate experiment is directly influenced. Some plate washers fail to achieve a sufficiently low residual volume and also result in a high background or a large standard deviation. In order to ensure that the cleaning data of the light-emitting plate is accurate and reliable and provide more reliable data for medical inspection, the method has very important significance for measuring the volume of the residual liquid in a single hole of the light-emitting plate after the plate washer is washed.
Because the capacity of each hole of a 96-hole plate is very small, about 350-.
Disclosure of Invention
The invention aims to provide a method for measuring the volume of the single-hole residual liquid of the light-emitting panel, which is simple to operate and accurate in result.
In order to achieve the purpose, the invention can adopt the following technical scheme:
the invention relates to a method for measuring the volume of single-hole residual liquid of a light-emitting panel, which comprises the following steps:
firstly, adding luminescent substrates into coating holes of a blank luminescent plate according to a1 mu l, a2 mu l and a3 mu l per hole, wherein a1 is more than a2 and more than a3.. Respectively adding equal amount of enzyme into the coating holes added with the luminescent substrate, and then measuring the luminescent value of each hole by using a luminometer;
secondly, establishing a standard curve by taking the logarithm of the volume quantity of the luminescent substrate as an abscissa (X axis) and the luminescent value as an ordinate (Y axis);
thirdly, replacing the washing liquor of the plate washing machine with a luminescent substrate to clean the blank luminescent plate;
fourthly, after the cleaning is finished, adding equal amount of enzyme into each coating hole of the luminescent plate, and measuring the luminous value of each hole by using a luminometer;
and fifthly, bringing the light emitting value of each hole into the standard curve established in the second step in sequence to obtain the volume of the residual liquid in each coated hole in the light emitting panel.
The method has the advantages that the method is simple, the volume of residual liquid in each coating hole of the light-emitting panel after being cleaned by the panel washer can be accurately measured, the volume of trace liquid below 1 mu l can be measured if necessary, the accuracy and reliability of the cleaning data of the light-emitting panel are ensured, and meanwhile, the reliable data are provided for medical inspection.
Drawings
FIG. 1 is a schematic representation of the sample loading layout in the example.
Fig. 2 is a fitted standard curve.
Detailed Description
The process of the present invention is described in more detail below by way of specific examples to facilitate understanding by those skilled in the art.
The method for measuring the volume of the lighting plate single-hole residual liquid comprises the following specific steps:
step one, taking 1ml of luminescent substrate A and 1ml of luminescent substrate B, uniformly mixing, adding the uniformly mixed luminescent substrates into coating holes with blank luminescent plates according to a sample adding layout shown in figure 1 (0, 2, 5, 7, 10 and 15 in figure 1 respectively represent that the sample adding amount of the luminescent substrates is 0 mul, 2 mul, 5 mul, 7 mul, 10 mul and 15 mul), then respectively adding 20 mul of enzyme conjugates into the coating holes with the luminescent substrates, and measuring the luminescent value of each hole by using a luminometer after 5-10 minutes; the method comprises the following steps of enabling a luminous substrate to release photons through the catalytic action of enzyme, measuring the intensity of the released photons by using a luminometer, and when the amount of the enzyme is fixed, enabling the amount of the luminous substrate to be in a direct proportion relation with the luminous intensity detected by the luminometer;
secondly, establishing a standard curve by taking the logarithm of the volume quantity of the luminescent substrate as an abscissa (X axis) and the luminescent value as an ordinate (Y axis), as shown in FIG. 2;
thirdly, replacing the washing liquor of the plate washing machine with a luminescent substrate, and cleaning the blank luminescent plate (normally cleaning according to the kit specification);
fourthly, after the cleaning is finished, respectively adding 20 mul of enzyme conjugate into the coating holes of the luminescent plate, and then measuring the luminous intensity of each hole of photon by using a luminometer after the luminescent substrate releases photons through the catalytic action of enzyme;
and fifthly, bringing the luminous intensity of each hole into the standard curve established in the second step in sequence to obtain the volume of the residual liquid in each coating hole in the luminous plate.
Claims (1)
1. A method for measuring the volume of the lighting plate single-hole residual liquid is characterized in that: the method comprises the following steps: firstly, adding luminescent substrates into coating holes of a blank luminescent plate according to a1 mu l, a2 mu l and a3 mu l per hole, wherein a1 is more than a2 and more than a3.. Respectively adding equal amount of enzyme into the coating holes added with the luminescent substrate, and then measuring the luminescent value of each hole by using a luminometer; secondly, establishing a standard curve by taking the logarithm of the volume quantity of the luminescent substrate as an abscissa and the luminescent value as an ordinate; thirdly, replacing the washing liquor of the plate washing machine with a luminescent substrate to clean the blank luminescent plate; fourthly, after the cleaning is finished, adding equal amount of enzyme into each coating hole of the luminescent plate, and measuring the luminous value of each hole by using a luminometer; and fifthly, bringing the light emitting value of each hole into the standard curve established in the second step in sequence to obtain the volume of the residual liquid in each coated hole in the light emitting panel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811441555.4A CN109307541B (en) | 2018-11-29 | 2018-11-29 | Method for measuring volume of lighting plate single-hole residual liquid |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811441555.4A CN109307541B (en) | 2018-11-29 | 2018-11-29 | Method for measuring volume of lighting plate single-hole residual liquid |
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| Publication Number | Publication Date |
|---|---|
| CN109307541A CN109307541A (en) | 2019-02-05 |
| CN109307541B true CN109307541B (en) | 2020-03-24 |
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| CN201811441555.4A Active CN109307541B (en) | 2018-11-29 | 2018-11-29 | Method for measuring volume of lighting plate single-hole residual liquid |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN206019759U (en) * | 2016-08-24 | 2017-03-15 | 深圳市有为环境科技有限公司 | Possesses the photoelectric metering instrument of defoaming function |
| CN108139323A (en) * | 2015-10-09 | 2018-06-08 | 微密斯点胶技术有限公司 | For the light guide body unit of optical drop detection |
| CN108333379A (en) * | 2007-10-02 | 2018-07-27 | 赛拉诺斯知识产权有限责任公司 | Modular point-of-care devices and its application |
| CN108871518A (en) * | 2017-05-15 | 2018-11-23 | 富泰华工业（深圳）有限公司 | Amount of drinking water monitors system and amount of drinking water monitoring device |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1278821A4 (en) * | 2000-05-03 | 2005-11-09 | Jen Gau Jr | BIOLOGICAL IDENTIFICATION SYSTEM WITH INTEGRATED SENSOR CHIP |
| AU2001295361A1 (en) * | 2000-11-17 | 2002-05-27 | Tecan Trading Ag | Method and device for determining the volume of a sample of a liquid |
| CN104520706B (en) * | 2012-04-17 | 2017-03-01 | 印度理工学院 | Usage amount submanifold detects discharge |
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- 2018-11-29 CN CN201811441555.4A patent/CN109307541B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108333379A (en) * | 2007-10-02 | 2018-07-27 | 赛拉诺斯知识产权有限责任公司 | Modular point-of-care devices and its application |
| CN108139323A (en) * | 2015-10-09 | 2018-06-08 | 微密斯点胶技术有限公司 | For the light guide body unit of optical drop detection |
| CN206019759U (en) * | 2016-08-24 | 2017-03-15 | 深圳市有为环境科技有限公司 | Possesses the photoelectric metering instrument of defoaming function |
| CN108871518A (en) * | 2017-05-15 | 2018-11-23 | 富泰华工业（深圳）有限公司 | Amount of drinking water monitors system and amount of drinking water monitoring device |
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