CN109234187A - 一株莠去津降解菌及其应用 - Google Patents
一株莠去津降解菌及其应用 Download PDFInfo
- Publication number
- CN109234187A CN109234187A CN201810930113.XA CN201810930113A CN109234187A CN 109234187 A CN109234187 A CN 109234187A CN 201810930113 A CN201810930113 A CN 201810930113A CN 109234187 A CN109234187 A CN 109234187A
- Authority
- CN
- China
- Prior art keywords
- atrazine
- degradation bacteria
- application
- plant
- microbial inoculum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- MXWJVTOOROXGIU-UHFFFAOYSA-N atrazine Chemical compound CCNC1=NC(Cl)=NC(NC(C)C)=N1 MXWJVTOOROXGIU-UHFFFAOYSA-N 0.000 title claims abstract description 50
- 241000894006 Bacteria Species 0.000 title claims abstract description 25
- 230000015556 catabolic process Effects 0.000 title claims abstract description 20
- 238000006731 degradation reaction Methods 0.000 title claims abstract description 20
- 230000006872 improvement Effects 0.000 claims abstract description 6
- 241000056156 Methylopila sp. Species 0.000 claims abstract description 5
- 239000002068 microbial inoculum Substances 0.000 claims description 8
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 239000000470 constituent Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 5
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 abstract description 3
- 238000011084 recovery Methods 0.000 abstract description 2
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 15
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 9
- 239000002609 medium Substances 0.000 description 9
- 230000001954 sterilising effect Effects 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 239000007788 liquid Substances 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 238000004587 chromatography analysis Methods 0.000 description 4
- 239000011550 stock solution Substances 0.000 description 4
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- 239000007836 KH2PO4 Substances 0.000 description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 108020004465 16S ribosomal RNA Proteins 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 229910052564 epsomite Inorganic materials 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 238000005374 membrane filtration Methods 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 230000009182 swimming Effects 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 206010049290 Feminisation acquired Diseases 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000881769 Methylopila Species 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 231100000674 Phytotoxicity Toxicity 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000010086 Setaria viridis var. viridis Nutrition 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 235000019504 cigarettes Nutrition 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 239000000598 endocrine disruptor Substances 0.000 description 1
- 238000010812 external standard method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 244000230342 green foxtail Species 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 239000012074 organic phase Substances 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000009418 renovation Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 230000033772 system development Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/04—Pesticides, e.g. insecticides, herbicides, fungicides or nematocides
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/20—Organic substances
- A62D2101/22—Organic substances containing halogen
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/20—Organic substances
- A62D2101/26—Organic substances containing nitrogen or phosphorus
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Molecular Biology (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Business, Economics & Management (AREA)
- Emergency Management (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
本发明涉及一株莠去津降解菌及其应用,该菌株为Methylopila sp.Y6X‑2,保藏于中国微生物菌种保藏管理委员会普通微生物中心,保藏中心登记入册编号为:CGMCC No.15919。本发明菌能够降解莠去津,可应用于莠去津污染环境治理修复技术领域。
Description
技术领域
本发明属于环境微生物领域,具体涉及一株莠去津降解菌及其应用。
背景技术
莠去津的杀草谱较广,可防除多种一年生禾本科和阔叶杂草,尤其对玉米有较好的选择性(因玉米体内有解毒机制),我国在70年代开始生产并大规模应用。但莠去津具有明显的“三致”作用,可引起雄蛙雌化,美国、欧盟和日本均已将其列入内分泌干扰化合物名单,长期暴露于莠去津能引起人类和其他动物种类的健康风险。目前关于莠去津的药害,除了预防,还可以采用生物修复方法对污染环境进行治理,因此获得莠去津降解菌株用于污染环境治理修复具有重要意义。
发明内容
本发明的目的是提供一株莠去津降解菌及其应用。
本发明采用的具体方案为:
本发明提供了一株莠去津降解菌,该菌株为Methylopila sp.Y6X-2,保藏于中国微生物菌种保藏管理委员会普通微生物中心,保藏中心登记入册编号为:CGMCC No.15919。
本发明还提供了上述莠去津降解菌的应用,将所述的莠去津降解菌接种于莠去津污染环境中,进行治理修复。
本发明还提供了一种菌剂,该菌剂的活性成分为上述莠去津降解菌。
本发明还提供了上述菌剂的应用,将所述的菌剂接种于莠去津污染环境中,进行治理修复。
本发明所具有的有益效果:
本发明菌能够降解莠去津,可应用于莠去津污染环境治理修复技术领域。
附图说明
图1为莠去津降解菌Y6X-2的PCR产物电泳图;
图2为菌株Y6X-2与相关种的16S rDNA序列系统发育树。
菌株保藏
本发明Methylopila sp.Y6X-2,已保藏于中国微生物菌种保藏管理委员会普通微生物中心,保藏地址:北京市朝阳区北辰西路1号院3号,保藏中心登记入册编号为CGMCCNo.15919,保藏日期:2018年6月8日。
具体实施方式
下面结合具体实施例对本发明做进一步说明,但不限定本发明的保护范围。
实施例1:莠去津降解菌Y6X-2的分离
将天津蓟州区良种试验场莠去津污染的土样按5%的量加入灭菌的100ml含有200mg/L莠去津液体富集培养基中,在30℃、150rpm振荡培养。培养7d后从培养液中取出5ml转入100mL新鲜的含有200mg/L莠去津富集培养基中,在30℃、150rpm振荡培养。再次培养7d后从培养液中取出5ml转入100mL新鲜的含有200mg/L莠去津富集培养基中,在30℃、150rpm振荡培养。继续培养7d后取培养液逐级稀释,涂布于添加200mg/L莠去津的固体富集培养基平板上,30℃培养一段时间后,将能产生透明圈的菌落分离出来进行纯化。纯化得到菌株Y6X-2。
其中液体富集培养基:将莠去津(97%,购自国药集团化学试剂有限公司)配成10g/L的甲醇储备液,经0.22μm孔径的滤膜过滤除菌。配制培养基时,按浓度取储备液于一个灭菌的三角瓶中,在超净台中放置挥发甲醇。然后加入100ml灭菌的无机盐培养基(K2HPO41.79g/L、KH2PO4 0.45g/L、MgSO4·7H2O 0.29g/L、NaCl 0.4g/L、其余为水,pH 7),摇匀。
其中固体富集培养基:在液体富集培养基基础上加入1.5%的琼脂。
实施例2莠去津降解菌Y6X-2的鉴定
将活化的Y6X-2,CGMCC No.15919接种在液体培养培养基(蛋白胨0.5%、氯化钠0.5%,酵母粉0.25%,其余为水,灭菌冷却后加入1%的已过滤除菌的甲醇)中,30℃、150rpm振荡培养24h。取1ml新鲜培养菌液4℃、8000rpm离心5min,收集菌体于2ml离心管中,采用DNA提取试剂盒提取DNA。电泳检测后,采用通用引物8F/1492R进行PCR扩增。PCR产物电泳检测后,如图1泳道4电泳条带,测定16S rDNA基因序列,具体序列如SEQ ID NO:1所示,经过比对后属于Methylopila菌。Y6X-2的形态特征:短杆状、不产生芽孢,在固体平板上形成直径1-2mm的细小白色菌落。Y6X-2的生理生化特征:革兰氏染色阴性,脲酶反应阳性。
序列表:SEQ ID NO:1:
gagcggcagacgggtgagtaacgcgtggggatgtgccttgtggtacggaacaactcagggaaacttgagctaataccgtataagcccttcgggggaaagatttatcgccacaagatcaacccgcgttggattagctagttggtgaggtaacggctcaccaaggcgacgatccatagctggtctgagaggatgatcagccacactgggactgagacacggcccagactcctacgggaggcagcagtggggaatattggacaatgggcgcaagcctgatccagccatgccgcgtgagtgatgaaggccttagggttgtaaagctctttcactggggaagataatgacggtacccagagaagaagccccggctaacttcgtgccagcagccgcggtaatacgaagggggctagcgttgttcggaatcactgggcgtaaagcgcacgtaggcggacttttaagtcaggggtgaaatcccaaggctcaaccttggaactgcctttgatactggaagtcttgagttcgggagaggtgagtggaactgcgagtgtagaggtgaaattcgtagatattcgcaagaacaccagtggcgaaggcggctcactggcccgatactgacgctgaggtgcgaaagcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatggaagctagccgttggtcagcatgctgatcagtggcgcagctaacgctttaagcttcccgcctggggagtacggtcgcaagattaaaactcaaaggaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgaagcaacgcgcagaaccttaccagctcttgacatgccgtgttacccggagagattcggggtccacttcggtggcgcggacacaggtgctgcatggctgtcgtcagctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaaccctcgcccctagttgccatcattcagttgggcactctagggggactgccggtgataagccgagaggaaggtggggatgacgtcaagtcctcatggcccttacgggctgggctacacacgtgctacaatggcggtgacagtgggcagcaaaggggcgacccctagctaatctccaaaagccgtctcagttcggattgcactctgcaactcgagtgcatgaaggtggaatcgctagtaatcgcggatcagcatgccgcggtgaatacgttcccgggccttgtacacaccgcccgtcacaccatgggagttggttttacccgaaggcgc
实施例3:Y6X-2,CGMCC No.15919降解莠去津
将活化的Y6X-2,CGMCC No.15919接种在液体培养培养基(蔗糖130g/L、蛋白胨2g/L、Na2HPO4 1.4g/L、KH2PO4 0.3g/L、其余为水,pH 7)中,30℃、150rpm振荡培养24h。按2%(V/V)取新鲜菌液接种在含有200mg/L莠去津的液体富集培养基中,在30℃、150rpm振荡培养7d。然后用等体积二氯甲烷萃取3次,弃水相,合并有机相,经无水硫酸钠脱水后用旋转蒸发仪减压浓缩至干,用色谱乙腈定容至10mL,过0.22μm有机滤膜。
使用文献“烟嘧-莠去津油悬浮剂的高效液相色谱分析”中的高效液相色谱法检测莠去津的残余量。首先采用外标法定量,以色谱乙腈为溶剂将莠去津标准品配制成500mg/L(取0.01g莠去津原药,溶解于20mL的色谱乙腈)的母液,然后依次稀释成0、10mg/L、20mg/L、30mg/L、40mg/L、50mg/L、75mg/L、100mg/L 8个浓度梯度,分别测定峰面积,结果见表1。以莠去津浓度为横坐标,对应的峰面积为纵坐标,绘制标准曲线得曲线公式:y=35716x+16022,其中R2=0.9989。
表1不同莠去津浓度(mg/L)及峰面积
| 浓度 | 0 | 10 | 20 | 30 | 40 | 50 | 75 | 100 |
| 峰面积 | 0 | 410484 | 753533 | 1071435 | 1475165 | 1735429 | 2654747 | 3634987 |
使用同样的方法测定Y6X-2培养后的莠去津浓度对应的峰面积为439614,带入标准曲线公式计算对应的莠去津浓度为11.86mg/L。
根据(起始莠去津浓度-培养后莠去津浓度)/起始莠去津浓度计算Y6X-2,CGMCCNo.15919降解莠去津去除率=(200-11.86)/200=94.07%。
其中液体富集培养基:将莠去津(97%,购自国药集团化学试剂有限公司)配成10g/L的甲醇储备液,经0.22μm孔径的滤膜过滤除菌。配制培养基时,按浓度取储备液于一个灭菌的三角瓶中,在超净台中放置挥发甲醇。然后加入100ml灭菌的无机盐培养基(K2HPO41.79g/L、KH2PO4 0.45g/L、MgSO4·7H2O 0.29g/L、NaCl 0.4g/L、其余为水,pH 7),摇匀。
序列表
<110> 李晓明
<120> 一株莠去津降解菌及其应用
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1299
<212> DNA
<213> 甲基球状菌(Methylopila sp.)
<400> 1
gagcggcaga cgggtgagta acgcgtgggg atgtgccttg tggtacggaa caactcaggg 60
aaacttgagc taataccgta taagcccttc gggggaaaga tttatcgcca caagatcaac 120
ccgcgttgga ttagctagtt ggtgaggtaa cggctcacca aggcgacgat ccatagctgg 180
tctgagagga tgatcagcca cactgggact gagacacggc ccagactcct acgggaggca 240
gcagtgggga atattggaca atgggcgcaa gcctgatcca gccatgccgc gtgagtgatg 300
aaggccttag ggttgtaaag ctctttcact ggggaagata atgacggtac ccagagaaga 360
agccccggct aacttcgtgc cagcagccgc ggtaatacga agggggctag cgttgttcgg 420
aatcactggg cgtaaagcgc acgtaggcgg acttttaagt caggggtgaa atcccaaggc 480
tcaaccttgg aactgccttt gatactggaa gtcttgagtt cgggagaggt gagtggaact 540
gcgagtgtag aggtgaaatt cgtagatatt cgcaagaaca ccagtggcga aggcggctca 600
ctggcccgat actgacgctg aggtgcgaaa gcgtggggag caaacaggat tagataccct 660
ggtagtccac gccgtaaacg atggaagcta gccgttggtc agcatgctga tcagtggcgc 720
agctaacgct ttaagcttcc cgcctgggga gtacggtcgc aagattaaaa ctcaaaggaa 780
ttgacggggg cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa cgcgcagaac 840
cttaccagct cttgacatgc cgtgttaccc ggagagattc ggggtccact tcggtggcgc 900
ggacacaggt gctgcatggc tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg 960
caacgagcgc aaccctcgcc cctagttgcc atcattcagt tgggcactct agggggactg 1020
ccggtgataa gccgagagga aggtggggat gacgtcaagt cctcatggcc cttacgggct 1080
gggctacaca cgtgctacaa tggcggtgac agtgggcagc aaaggggcga cccctagcta 1140
atctccaaaa gccgtctcag ttcggattgc actctgcaac tcgagtgcat gaaggtggaa 1200
tcgctagtaa tcgcggatca gcatgccgcg gtgaatacgt tcccgggcct tgtacacacc 1260
gcccgtcaca ccatgggagt tggttttacc cgaaggcgc 1299
Claims (4)
1.一株莠去津降解菌,其特征在于:该菌株为Methylopila sp.Y6X-2,保藏于中国微生物菌种保藏管理委员会普通微生物中心,保藏中心登记入册编号为:CGMCC No.15919。
2.权利要求1所述的莠去津降解菌的应用,其特征在于:将所述的莠去津降解菌接种于莠去津污染环境中,进行治理修复。
3.一种菌剂,其特征在于:该菌剂的活性成分为权利要求1所述的莠去津降解菌。
4.权利要求3所述菌剂的应用,其特征在于:将所述的菌剂接种于莠去津污染环境中,进行治理修复。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810930113.XA CN109234187B (zh) | 2018-08-15 | 2018-08-15 | 一株莠去津降解菌及其应用 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810930113.XA CN109234187B (zh) | 2018-08-15 | 2018-08-15 | 一株莠去津降解菌及其应用 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109234187A true CN109234187A (zh) | 2019-01-18 |
| CN109234187B CN109234187B (zh) | 2021-06-15 |
Family
ID=65070393
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810930113.XA Active CN109234187B (zh) | 2018-08-15 | 2018-08-15 | 一株莠去津降解菌及其应用 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109234187B (zh) |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1244771A (zh) * | 1996-11-29 | 2000-02-16 | 麦克罗弗罗公司 | 含有细菌的农用组合物 |
| CN101421412A (zh) * | 2004-12-17 | 2009-04-29 | 梅坦诺米克斯有限公司 | 控制精细化学品产生的方法 |
| CN102906032A (zh) * | 2010-05-28 | 2013-01-30 | 英派尔科技开发有限公司 | 用于氧化甲烷的系统和方法 |
| RU133126U1 (ru) * | 2013-06-21 | 2013-10-10 | Федеральное государственное бюджетное учреждение науки Институт биохимии и физиологии микроорганизмов им. Г.К. Скрябина Российской академии наук | Биосенсор для определения метиламина, диметиламина и триметиламина в водной среде |
| WO2015100431A2 (en) * | 2013-12-24 | 2015-07-02 | Symbiota, Inc. | Plants containing beneficial endophytes |
| CN105567597A (zh) * | 2016-01-15 | 2016-05-11 | 哈尔滨工业大学宜兴环保研究院 | 一株高效阿特拉津降解菌及其应用以及筛选方法 |
| CN105814197A (zh) * | 2013-08-12 | 2016-07-27 | 巴斯夫农业公司 | 具有增加的除草剂耐受性的植物 |
| CN106591179A (zh) * | 2016-12-05 | 2017-04-26 | 长兴制药股份有限公司 | 甲基包囊菌及其在选择性拆分制备(S)‑α‑乙基‑2‑氧‑1‑吡咯烷乙酸盐上的应用 |
| US20170223967A1 (en) * | 2014-06-26 | 2017-08-10 | Ait Austrian Institute Of Technology Gmbh | Plant-endophyte combinations and uses therefor |
| CN108220410A (zh) * | 2016-12-16 | 2018-06-29 | Md保健株式会社 | 用于通过细菌宏基因组分析来诊断胃癌的试剂盒和方法 |
| WO2018124606A1 (ko) * | 2016-12-26 | 2018-07-05 | 주식회사 엠디헬스케어 | 미생물 메타게놈 분석을 통한 유방암 진단방법 |
-
2018
- 2018-08-15 CN CN201810930113.XA patent/CN109234187B/zh active Active
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1244771A (zh) * | 1996-11-29 | 2000-02-16 | 麦克罗弗罗公司 | 含有细菌的农用组合物 |
| CN101421412A (zh) * | 2004-12-17 | 2009-04-29 | 梅坦诺米克斯有限公司 | 控制精细化学品产生的方法 |
| CN102906032A (zh) * | 2010-05-28 | 2013-01-30 | 英派尔科技开发有限公司 | 用于氧化甲烷的系统和方法 |
| RU133126U1 (ru) * | 2013-06-21 | 2013-10-10 | Федеральное государственное бюджетное учреждение науки Институт биохимии и физиологии микроорганизмов им. Г.К. Скрябина Российской академии наук | Биосенсор для определения метиламина, диметиламина и триметиламина в водной среде |
| CN105814197A (zh) * | 2013-08-12 | 2016-07-27 | 巴斯夫农业公司 | 具有增加的除草剂耐受性的植物 |
| WO2015100431A2 (en) * | 2013-12-24 | 2015-07-02 | Symbiota, Inc. | Plants containing beneficial endophytes |
| EP3086646A4 (en) * | 2013-12-24 | 2017-08-09 | Indigo AG, Inc. | Plants containing beneficial endophytes |
| US20170223967A1 (en) * | 2014-06-26 | 2017-08-10 | Ait Austrian Institute Of Technology Gmbh | Plant-endophyte combinations and uses therefor |
| CN105567597A (zh) * | 2016-01-15 | 2016-05-11 | 哈尔滨工业大学宜兴环保研究院 | 一株高效阿特拉津降解菌及其应用以及筛选方法 |
| CN106591179A (zh) * | 2016-12-05 | 2017-04-26 | 长兴制药股份有限公司 | 甲基包囊菌及其在选择性拆分制备(S)‑α‑乙基‑2‑氧‑1‑吡咯烷乙酸盐上的应用 |
| CN108220410A (zh) * | 2016-12-16 | 2018-06-29 | Md保健株式会社 | 用于通过细菌宏基因组分析来诊断胃癌的试剂盒和方法 |
| WO2018124606A1 (ko) * | 2016-12-26 | 2018-07-05 | 주식회사 엠디헬스케어 | 미생물 메타게놈 분석을 통한 유방암 진단방법 |
Non-Patent Citations (3)
| Title |
|---|
| CHAN, CY等: "Treatment of atrazine by integrating photocatalytic", 《ENVIRONMENTAL POLLUTION》 * |
| 姚红涛等: "Methylopila sp. YHT-1鉴定及其发酵产吡咯喹啉醌", 《食品与生物技术学报》 * |
| 黄星等: "降解菌S113对甲磺隆污染土壤生物修复作用的研究 ", 《土壤学报》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109234187B (zh) | 2021-06-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106497809B (zh) | 一种阴沟肠杆菌、含有该菌的菌剂及其应用和钝化汞的方法 | |
| CN106493167A (zh) | 地衣芽孢杆菌和菌剂及它们的应用和钝化重金属的方法 | |
| CN104531589B (zh) | 降解氯氰菊酯的鞘氨醇单胞菌及其应用 | |
| CN103031261B (zh) | 无色杆菌属d-12及其在微生物降解乙草胺中的应用 | |
| CN102181386B (zh) | 乙酰微小杆菌l31及其应用 | |
| CN115386520B (zh) | 一株嗜吡啶红球菌rl-gz01菌株及其应用 | |
| CN111560326B (zh) | 一株中间苍白杆菌Ochrobactrum intermedium 26B及其应用 | |
| CN104263682B (zh) | 一株具有多环芳烃降解功能的植物促生内生细菌及其应用 | |
| CN110078220B (zh) | 一种利用蓝藻原位修复砷污染高盐碱水的方法及菌株 | |
| CN102583780A (zh) | 嗜麦芽寡养单胞菌ds4在降解皂素废水有机污染物中的应用 | |
| CN116064296B (zh) | 一株硝磺草酮降解菌Klebsiella pasteurii CM-1及应用 | |
| CN109929785B (zh) | 一株能够降解2,6-二甲基苯酚的细菌及其生产的菌剂 | |
| CN105199981A (zh) | 食碱戈登氏菌yc-rl2及其应用 | |
| CN110577908A (zh) | 一种拟除虫菊酯类杀虫剂的降解菌株及其应用 | |
| CN103695342A (zh) | 一株具有溶藻活性的芽孢杆菌及其应用 | |
| CN106119167B (zh) | 一种利用新微生物菌株降解土壤中三唑醇的方法 | |
| CN105154365A (zh) | 分枝杆菌yc-rl4及其应用 | |
| CN105199988B (zh) | 一株具有菲降解功能的根表成膜细菌rs2及其应用 | |
| CN109234187A (zh) | 一株莠去津降解菌及其应用 | |
| CN115975891B (zh) | 降解n-甲基吡咯烷酮的苍白杆菌及其在废水处理中的应用 | |
| CN117363533A (zh) | 一株蜡样芽孢杆菌及其在苯甲酸降解中的应用 | |
| CN117247864A (zh) | 一株氏普罗菲登斯菌a2菌株及其应用 | |
| CN1570079A (zh) | 一株硝基苯类污染物高效降解菌及其使用方法 | |
| CN115340966A (zh) | 一种戈登氏菌及其应用 | |
| CN102352326B (zh) | 一种利用气单胞菌去除水华蓝藻的方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |