CN109234161A - A kind of rocking equipment and its application method of stem cell medium - Google Patents
A kind of rocking equipment and its application method of stem cell medium Download PDFInfo
- Publication number
- CN109234161A CN109234161A CN201811115413.9A CN201811115413A CN109234161A CN 109234161 A CN109234161 A CN 109234161A CN 201811115413 A CN201811115413 A CN 201811115413A CN 109234161 A CN109234161 A CN 109234161A
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- stem cell
- groups
- rectangular channel
- oscillating plate
- cell medium
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention provides a kind of rocking equipment of stem cell medium, including shell and oscillating plate, the oscillating plate upper surface offers rectangular channel, the rectangular channel upper inside is equipped with porous plate by bolt, the porous plate upper surface is symmetrically installed two groups of cylinders by bolt, three groups of threaded rods are mounted equidistant in cylinder upper end outer surface in a ring, described threaded rod one end rubber sleeve is pasted by glue, left side opens up through-hole inside the rectangular channel, and brush roll is set in rectangular channel, the brush roll annular side medium position steady brace, the pull rod left end passes through through-hole and extends on the left of oscillating plate, compared with prior art, the present invention is with following the utility model has the advantages that realizing the purpose of the stem cell medium bottle of clamping different-diameter using three groups of threaded rods on cylinder, realize that collecting stem cell cultivates using rectangular channel The liquid of liquid bottle cracking leakage, reduces influence of the liquid to working environment of leakage.
Description
Technical field
The present invention is the rocking equipment and its application method of a kind of stem cell medium, belongs to biotechnological device neck
Domain.
Background technique
Stem cell medium is required specific culture environment in the incubation for act on stem cell, is had reached dry thin
The adherent or value-added effect of born of the same parents is easy in the case where standing for a long time however, traditional stem cell medium is in process of production
It precipitates, react uneven and influences the culture effect to stem cell.
Summary of the invention
In view of the deficienciess of the prior art, it is an object of the present invention to provide a kind of rocking equipment of stem cell medium and
Its application method, to solve the problems mentioned in the above background technology, operation of the present invention is simple, has leakage collecting function, just
It is applied widely in cleaning.
To achieve the goals above, the present invention is to realize by the following technical solutions: a kind of stem cell medium is used
Rocking equipment, including shell, oscillating plate, spring and small vibration motor, oscillating plate setting upside inside housings,
The spring upper end is fixed on oscillating plate lower end surface, and lower spring end is immobilized in the shell subordinate end face, the small vibrating electricity
Machine is mounted on oscillating plate lower end surface by bolt, and the oscillating plate upper surface offers rectangular channel, the rectangular channel upper inside
Porous plate is installed by bolt, the porous plate upper surface is symmetrically installed two groups of cylinders by bolt, on the cylinder
Three groups of threaded rods are mounted equidistant in end outer surface in a ring, and rubber sleeve, the rubber sleeve are pasted by glue in described threaded rod one end
It is arranged in cylinder, left side opens up through-hole inside the rectangular channel, and brush roll, the hairbrush collars are arranged in rectangular channel
Shape side medium position steady brace, the pull rod left end pass through through-hole and extend on the left of oscillating plate, the oscillating plate left side
Drain pipe is installed and drain pipe right end extends in rectangular channel, drain pipe left end installed plug.
Further, cylinder upper end outer surface equidistantly offers three groups of threaded holes in a ring, and the threaded rod is logical
It crosses and is threadably engaged in threaded hole.
Further, the pull rod left end is pasted with rubber stopper by glue.
Further, machining internal thread on the left of the drain pipe internal surface, threading in the plug annular side,
The plug is fixedly connected by external screw thread and internal screw thread with drain pipe.
Further, the housing forward end face is equipped with ship type switch, and the ship type switch passes through conducting wire and small vibrating
Electrical connection of motor.
Further, the spring is equipped with four groups, and spring described in four groups is arranged in the rib of oscillating plate lower end surface four
At angle.
A kind of application method of the rocking equipment of stem cell medium, comprising the following steps: connect small vibration motor
Circuit between external power supply, small vibration motor, which is powered to generating, to be vibrated and drives vibration panel vibration, and oscillating plate upper surface is opened
Equipped with rectangular channel, rectangular channel upper inside is equipped with porous plate by bolt, and porous plate upper surface is symmetrically installed two by bolt
Group cylinder, three groups of threaded rods are mounted equidistant in a ring, stem cell medium bottle is placed on cylinder for cylinder upper end outer surface
In vivo, three groups of threaded rods are then turned, until three groups of rubber sleeves are in contact with stem cell medium bottle, and then it is solid to complete clamping
It is fixed, it can be changed because threaded rod is in the intracorporal length of cylinder, therefore the stem cell medium bottle of a variety of different-diameters can be placed in
In cylinder.
Beneficial effects of the present invention: because the present invention is added to cylinder, threaded rod and rubber sleeve, which utilizes cylinder
Three groups of threaded rods on body realize the purpose of the stem cell medium bottle of clamping different-diameter, expand clamping range, rubber sleeve
Design reduce damage of the threaded rod to stem cell medium bottle.
Because the present invention is added to brush roll, porous plate, rectangular channel, pull rod, drain pipe and plug, which utilizes rectangle
Slot realizes the liquid for collecting the leakage of stem cell medium bottle cracking, reduces influence of the liquid to working environment of leakage, leads to
It crosses pull rod pulling brush roll and is slided in rectangular channel and achieve the purpose that cleaning, improve the convenience of rectangular channel internal cleaning, lead to
It crosses and removes plug and achieve the purpose that leakage and cleaning solution is discharged from drain pipe, operation of the present invention is simple, there is leakage collecting function,
It is applied widely convenient for cleaning.
Detailed description of the invention
Upon reading the detailed description of non-limiting embodiments with reference to the following drawings, other feature of the invention,
Objects and advantages will become more apparent upon:
Fig. 1 is a kind of structural schematic diagram of the rocking equipment of stem cell medium of the present invention；
Fig. 2 is the assembling schematic diagram of oscillating plate and shell in a kind of rocking equipment of stem cell medium of the present invention；
Fig. 3 is the assembling schematic diagram of threaded rod and cylinder in a kind of rocking equipment of stem cell medium of the present invention；
In figure: 1- shell, 2- oscillating plate, 3- spring, 4- small vibration motor, 5- brush roll, 6- porous plate, 7- rectangular channel, 8-
Pull rod, 9- through-hole, 10- drain pipe, 11- plug, 12- cylinder, 13- threaded rod, 14- rubber sleeve.
Specific embodiment
To be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, below with reference to
Specific embodiment, the present invention is further explained.
Fig. 1-Fig. 3 is please referred to, the present invention provides a kind of technical solution: a kind of rocking equipment of stem cell medium, packet
Shell 1, oscillating plate 2, spring 3 and small vibration motor 4 are included, for the setting of oscillating plate 2 in 1 upper inside of shell, 3 upper end of spring is solid
It is scheduled on 2 lower end surface of oscillating plate, and 3 lower end of spring is fixed on 1 interior lower end face of shell, oscillating plate 2 and shell are realized in the design of spring 3
The soft connection of body 1, small vibration motor 4 are mounted on 2 lower end surface of oscillating plate by bolt, connect small vibration motor 4 and outer
The circuit between power supply is connect, small vibration motor 4, which is powered to generating, to be vibrated and oscillating plate 2 is driven to vibrate, and 2 upper surface of oscillating plate opens up
There is rectangular channel 7,7 upper inside of rectangular channel is equipped with porous plate 6 by bolt, and 6 upper surface of porous plate is symmetrically installed by bolt
Three groups of threaded rods 13 are mounted equidistant in two groups of cylinders 12,12 upper end outer surface of cylinder in a ring, and 13 one end of threaded rod passes through glue
Water pastes rubber sleeve 14, and rubber sleeve 14 is arranged in cylinder 12, stem cell medium bottle is placed in cylinder 12, then
Three groups of threaded rods 13 are turned, until three groups of rubber sleeves 14 are in contact with stem cell medium bottle, and then complete to grip, because
It is changeable that threaded rod 13 is in the length in cylinder 12, therefore the stem cell medium bottle of a variety of different-diameters can be placed in circle
In cylinder 12, then expanding the scope of application, 7 inside left side of rectangular channel opens up through-hole 9, and brush roll 5 is arranged in rectangular channel 7,
5 annular side medium position steady brace 8 of brush roll, 8 left end of pull rod pass through through-hole 9 and extend to 2 left side of oscillating plate, oscillating plate 2
Left side installs drain pipe 10 and 10 right end of drain pipe extends in rectangular channel 7,10 left end installed plug 11 of drain pipe, when dry thin
When born of the same parents' culture jar cracking, stem cell medium is flowed into rectangular channel 7 under self gravitation effect by porous plate 6, is taken
Plug 11 on lower drain pipe 10, and then the liquid in rectangular channel 7 is discharged by drain pipe 10, when needing to scrub rectangular channel 7, edge
Through-hole 9 back and forth pull pull rod 8, and then pull rod 8 drive brush roll 5 slided in rectangular channel 7, then brush roll 5 is to rectangular channel 7
It is scrubbed, the convenience scrubbed so as to improve rectangular channel 7.
12 upper end outer surface of cylinder equidistantly offers three groups of threaded holes in a ring, and threaded rod 13 is by being threadably engaged in spiral shell
In pit, the assembly of three groups of threaded rods 13 is convenient in the design of three groups of threaded holes.
8 left end of pull rod is pasted with rubber stopper by glue, and rubber stopper is convenient for through-hole 9 being blocked and limited pull rod 8
The position of left end.
Machining internal thread on the left of drain pipe 10 inner surface, threading in 11 annular side of plug, plug 11 passes through outer
Screw thread and internal screw thread are fixedly connected with drain pipe 10, internal screw thread and externally threaded design tearing open convenient for plug 11 and drain pipe 10
Dress.
1 front end face of shell is equipped with ship type switch, and ship type switch is electrically connected by conducting wire and small vibration motor 4, ship
The work of control small vibration motor 4 is convenient in the design of type switch.
Spring 3 is equipped with four groups, and four groups of springs 3 are arranged in oscillating plate 2 lower end surface four edges and corners, four groups of springs 3
Realize uniform support.
A kind of application method of the rocking equipment of stem cell medium, comprising the following steps: connect small vibration motor 4
Circuit between external power supply, small vibration motor 4, which is powered to generating, to be vibrated and oscillating plate 2 is driven to vibrate, 2 upper surface of oscillating plate
Rectangular channel 7 is offered, 7 upper inside of rectangular channel is equipped with porous plate 6 by bolt, and 6 upper surface of porous plate is symmetrical by bolt
Two groups of cylinders 12 are installed, three groups of threaded rods 13 are mounted equidistant in 12 upper end outer surface of cylinder in a ring, by stem cell medium
Bottle be placed in cylinder 12, then turn three groups of threaded rods 13, until three groups of rubber sleeves 14 with stem cell medium bottle phase
Contact, and then complete to grip, because threaded rod 13 is in, the length in cylinder 12 is changeable, therefore a variety of different-diameters is dry
Cell culture fluid bottle can be placed in cylinder 12.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention, for this field skill
For art personnel, it is clear that invention is not limited to the details of the above exemplary embodiments, and without departing substantially from spirit of the invention or
In the case where essential characteristic, the present invention can be realized in other specific forms.Therefore, in all respects, should all incite somebody to action
Embodiment regards exemplary as, and is non-limiting, the scope of the present invention by appended claims rather than on state
Bright restriction, it is intended that including all changes that fall within the meaning and scope of the equivalent elements of the claims in the present invention
It is interior.Any reference signs in the claims should not be construed as limiting the involved claims.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (7)
1. a kind of rocking equipment of stem cell medium, including shell, oscillating plate, spring and small vibration motor, special
Sign is: upside, the spring upper end are fixed on oscillating plate lower end surface, and lower spring end inside housings for the oscillating plate setting
It is immobilized in the shell subordinate end face, the small vibration motor is mounted on oscillating plate lower end surface by bolt, on the oscillating plate
End face offers rectangular channel, and the rectangular channel upper inside is equipped with porous plate by bolt, and the porous plate upper surface passes through
Bolt is symmetrically installed two groups of cylinders, and three groups of threaded rods, the screw thread is mounted equidistant in cylinder upper end outer surface in a ring
Rubber sleeve is pasted by glue in bar one end, and the rubber is set in cylinder, and left side opens up logical inside the rectangular channel
Hole, and brush roll is set in rectangular channel, the brush roll annular side medium position steady brace, the pull rod left end passes through logical
Hole extends on the left of oscillating plate, and drain pipe is installed in the oscillating plate left side and drain pipe right end extends in rectangular channel, described
Drain pipe left end installed plug.
2. a kind of rocking equipment of stem cell medium according to claim 1, it is characterised in that: on the cylinder
End outer surface equidistantly offers three groups of threaded holes in a ring, and the threaded rod is by being threadably engaged in threaded hole.
3. a kind of rocking equipment of stem cell medium according to claim 1, it is characterised in that: the pull rod left end
Rubber stopper is pasted with by glue.
4. a kind of rocking equipment of stem cell medium according to claim 1, it is characterised in that: in the drain pipe
Machining internal thread on the left of surface, threading in the plug annular side, the plug by external screw thread and internal screw thread with
Drain pipe is fixedly connected.
5. a kind of rocking equipment of stem cell medium according to claim 1, it is characterised in that: the housing forward end
Face is equipped with ship type switch, and the ship type switch is electrically connected by conducting wire and small vibration motor.
6. a kind of rocking equipment of stem cell medium according to claim 1, it is characterised in that: the spring installation
There are four groups, spring described in four groups is arranged in the edges and corners of oscillating plate lower end surface four.
7. a kind of rocking equipment application method of -6 any stem cell mediums according to claim 1, feature exist
In: the following steps are included: connecting the circuit between small vibration motor and external power supply, small vibration motor, which is powered, generates vibration
And vibration panel vibration is driven, oscillating plate upper surface offers rectangular channel, and rectangular channel upper inside is equipped with porous plate by bolt,
Porous plate upper surface is symmetrically installed two groups of cylinders by bolt, and three groups of screw threads are mounted equidistant in cylinder upper end outer surface in a ring
Bar, stem cell medium bottle is placed in cylinder, then turns three groups of threaded rods, until three groups of rubber sleeves are and stem cell
Culture jar is in contact, and then completes to grip, and can be changed because threaded rod is in the intracorporal length of cylinder, therefore a variety of different straight
The stem cell medium bottle of diameter can be placed in cylinder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811115413.9A CN109234161A (en) | 2018-09-25 | 2018-09-25 | A kind of rocking equipment and its application method of stem cell medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811115413.9A CN109234161A (en) | 2018-09-25 | 2018-09-25 | A kind of rocking equipment and its application method of stem cell medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109234161A true CN109234161A (en) | 2019-01-18 |
Family
ID=65057314
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811115413.9A Pending CN109234161A (en) | 2018-09-25 | 2018-09-25 | A kind of rocking equipment and its application method of stem cell medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109234161A (en) |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4890748A (en) * | 1987-10-01 | 1990-01-02 | Visser John G | Product display and take-out cart |
| US6659637B2 (en) * | 2000-10-03 | 2003-12-09 | Union Scientific Corporation | Vertical electromagnetic shaker for biological and chemical specimens |
| CN103305424A (en) * | 2013-07-02 | 2013-09-18 | 黄山市中新生物科技有限公司 | Variable-amplitude rotary cell culture table |
| US8662791B2 (en) * | 2010-09-09 | 2014-03-04 | Impact Bidenergy LLC | Subterranean alternating digester system and method |
| CN204685112U (en) * | 2015-04-08 | 2015-10-07 | 大连北方成套教学设备有限公司 | Chemical laboratory gas defence, anti-leak, safe storage cabinet |
| CN205616906U (en) * | 2016-04-22 | 2016-10-05 | 广东普奥思生物科技有限公司 | Suspended cell culture deep hole dish innovation platform device |
| CN106399244A (en) * | 2016-10-28 | 2017-02-15 | 南通市宝通康生物工程股份有限公司 | NK cell medium |
| CN106906140A (en) * | 2017-02-28 | 2017-06-30 | 浙江兰博生物科技股份有限公司 | A kind of strain examination, cultural method and its complexes |
| WO2017203993A1 (en) * | 2016-05-27 | 2017-11-30 | Sumitomo Chemical Company, Limited | Spray device |
| CN206721182U (en) * | 2017-05-14 | 2017-12-08 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of superclean bench for yak cell culture |
| CN108048320A (en) * | 2017-12-23 | 2018-05-18 | 淮北智淮科技有限公司 | A kind of concussion frame of stem cell medium |
| CN207862352U (en) * | 2017-12-28 | 2018-09-14 | 广州楠泰生物科技有限公司 | A kind of cell incubator |
| CN207887090U (en) * | 2018-01-10 | 2018-09-21 | 谢珊 | One kind being convenient for fixed constant-temperature table |
-
2018
- 2018-09-25 CN CN201811115413.9A patent/CN109234161A/en active Pending
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4890748A (en) * | 1987-10-01 | 1990-01-02 | Visser John G | Product display and take-out cart |
| US6659637B2 (en) * | 2000-10-03 | 2003-12-09 | Union Scientific Corporation | Vertical electromagnetic shaker for biological and chemical specimens |
| US8662791B2 (en) * | 2010-09-09 | 2014-03-04 | Impact Bidenergy LLC | Subterranean alternating digester system and method |
| CN103305424A (en) * | 2013-07-02 | 2013-09-18 | 黄山市中新生物科技有限公司 | Variable-amplitude rotary cell culture table |
| CN204685112U (en) * | 2015-04-08 | 2015-10-07 | 大连北方成套教学设备有限公司 | Chemical laboratory gas defence, anti-leak, safe storage cabinet |
| CN205616906U (en) * | 2016-04-22 | 2016-10-05 | 广东普奥思生物科技有限公司 | Suspended cell culture deep hole dish innovation platform device |
| WO2017203993A1 (en) * | 2016-05-27 | 2017-11-30 | Sumitomo Chemical Company, Limited | Spray device |
| CN106399244A (en) * | 2016-10-28 | 2017-02-15 | 南通市宝通康生物工程股份有限公司 | NK cell medium |
| CN106906140A (en) * | 2017-02-28 | 2017-06-30 | 浙江兰博生物科技股份有限公司 | A kind of strain examination, cultural method and its complexes |
| CN206721182U (en) * | 2017-05-14 | 2017-12-08 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of superclean bench for yak cell culture |
| CN108048320A (en) * | 2017-12-23 | 2018-05-18 | 淮北智淮科技有限公司 | A kind of concussion frame of stem cell medium |
| CN207862352U (en) * | 2017-12-28 | 2018-09-14 | 广州楠泰生物科技有限公司 | A kind of cell incubator |
| CN207887090U (en) * | 2018-01-10 | 2018-09-21 | 谢珊 | One kind being convenient for fixed constant-temperature table |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190118 |