CN109168408A - Method for improving germination rate of vervain willow seeds - Google Patents
Method for improving germination rate of vervain willow seeds Download PDFInfo
- Publication number
- CN109168408A CN109168408A CN201811070038.0A CN201811070038A CN109168408A CN 109168408 A CN109168408 A CN 109168408A CN 201811070038 A CN201811070038 A CN 201811070038A CN 109168408 A CN109168408 A CN 109168408A
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- Prior art keywords
- seed
- willow leaf
- temperature
- environment
- percentage
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Links
- 235000018718 Verbena officinalis Nutrition 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 24
- 241000124033 Salix Species 0.000 title claims description 33
- 230000035784 germination Effects 0.000 title abstract description 8
- 241000424698 Verbena lasiostachys Species 0.000 title 1
- 240000001519 Verbena officinalis Species 0.000 claims abstract description 37
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims abstract description 22
- 235000010333 potassium nitrate Nutrition 0.000 claims abstract description 11
- 239000004323 potassium nitrate Substances 0.000 claims abstract description 11
- 230000007226 seed germination Effects 0.000 claims description 16
- 244000025254 Cannabis sativa Species 0.000 claims description 13
- 230000008014 freezing Effects 0.000 claims description 6
- 238000007710 freezing Methods 0.000 claims description 6
- BVPWJMCABCPUQY-UHFFFAOYSA-N 4-amino-5-chloro-2-methoxy-N-[1-(phenylmethyl)-4-piperidinyl]benzamide Chemical compound COC1=CC(N)=C(Cl)C=C1C(=O)NC1CCN(CC=2C=CC=CC=2)CC1 BVPWJMCABCPUQY-UHFFFAOYSA-N 0.000 claims description 5
- 241000196324 Embryophyta Species 0.000 claims description 5
- 238000011161 development Methods 0.000 claims description 5
- 230000018109 developmental process Effects 0.000 claims description 5
- 239000000428 dust Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 241001081203 Verbena Species 0.000 abstract 2
- 235000007212 Verbena X moechina Moldenke Nutrition 0.000 abstract 2
- 235000001594 Verbena polystachya Kunth Nutrition 0.000 abstract 2
- 235000007200 Verbena x perriana Moldenke Nutrition 0.000 abstract 2
- 235000002270 Verbena x stuprosa Moldenke Nutrition 0.000 abstract 2
- 238000004140 cleaning Methods 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 239000012535 impurity Substances 0.000 description 4
- 238000011534 incubation Methods 0.000 description 3
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000005057 refrigeration Methods 0.000 description 2
- 230000001139 anti-pruritic effect Effects 0.000 description 1
- 239000003908 antipruritic agent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- IRPDISVJRAYFBI-UHFFFAOYSA-N nitric acid;potassium Chemical compound [K].O[N+]([O-])=O IRPDISVJRAYFBI-UHFFFAOYSA-N 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for improving the germination rate of vervain seed, which comprises the following steps: (1) seed selection; (2) soaking seeds; (3) cleaning; (4) and (5) culturing. Has the advantages that: after the seeds of the vervain are treated by potassium nitrate, the germination rate of the seeds of the vervain can be improved from 1 percent to 85 to 95 percent; the method is simple and economic, is not influenced by factors such as seasons, climate, natural disasters and the like, can solve the problems of low germination rate and long germination time of the verbena salicifolia seeds by utilizing the technology, and has important significance for the production practice of the verbena salicifolia.
Description
Technical field:
The invention patent belongs to agriculture field, and in particular to a method of improve willow leaf Verbena officinalis percentage of seedgermination.
Background technique:
Verbena officinalis majority is grown on open country, and in China East China, the ground such as south China are also distributed, and with cool blood, dissipates the stasis of blood, clear
Heat, removing toxic substances, antipruritic and other effects, therefore become a kind of important draft；Simultaneously willow leaf Verbena officinalis due to its beautiful pattern it is wide
The general landscape that is applied to is arranged；The method that willow leaf Verbena officinalis is cultivated at present is that directly seed is put into culture dish, and culture dish is put
Into incubator, seed is allowed to grow naturally at normal temperature, is had the following problems using above-mentioned training method: in Seed Germination
In, since the dormant trait of willow leaf horsewhip grass seed is strong, allow it to germinate naturally at normal temperature, it is extremely difficult to break the suspend mode of seed, therefore
The seed of willow leaf Verbena officinalis is directly cultivated at normal temperature, and germination percentage only has 1%, this is serious to limit the life of leaf Verbena officinalis
It produces.
Summary of the invention:
The purpose of the present invention is to provide the methods of a kind of simple and economic raising willow leaf Verbena officinalis percentage of seedgermination.
The purpose of the present invention is implemented by following technical solution: a method of willow leaf Verbena officinalis percentage of seedgermination is improved,
The following steps are included:
(1) choose seeds: normal, the sufficiently mature willow leaf horsewhip grass seeds grain of selection development, removal foreign peoples plant grain, clast, dust
Equal impurity；
(2) it soaks seed: being placed on temperature after the seed chosen is mixed with the potassium nitrate solution that mass concentration is 0.01%-7%
To refrigerate 36-60h in 5 DEG C -10 DEG C of environment；
(3) it cleans: seed is pulled out from potassium nitrate solution, and rinsed 1-3 times with clear water, the seed cleaned is placed on
12h-36h is freezed in the environment that temperature is -1 DEG C -1 DEG C；
(4) it cultivates: the seed after freezing is uniformly spilt into the culture dish for being lined with 1-5 layers of wet filter paper；Then will
Culture dish is put into the environment that temperature is 20 DEG C -30 DEG C and carries out vernalization.
Further, the time of vernalization is 8-13 days in step 4.
Further, the seed to have germinateed in step 4 is taken out from culture dish, remaining non-chitting piece is put into temperature
In the environment that degree is 5 DEG C -10 DEG C, refrigerate 2-5 days；Then by culture dish be placed again into temperature be in 20 DEG C -30 DEG C of environment into
Row vernalization.
Further, the time of vernalization is 8-15 days.
Advantages of the present invention: the present invention provides a kind of methods for improving willow leaf Verbena officinalis percentage of seedgermination, utilize nitric acid
Potassium handles willow leaf horsewhip grass seed later using freezing and refrigeration, can break the suspend mode of seed, make willow leaf horsewhip grass seed
Germination percentage be increased to 85-95% from original 1%；The method of the invention is simple, it is economical, not by season, weather, natural calamity
The influence of the factors such as evil, can solve the problems, such as that willow leaf Verbena officinalis seed germination rate is low, sprout time is longer using this technology, right
The production practices of willow leaf Verbena officinalis are of great significance.
Specific embodiment:
It below will the present invention is described in further detail by embodiment.
Embodiment 1: a method of improving willow leaf Verbena officinalis percentage of seedgermination comprising following steps:
(1) choose seeds: normal, the sufficiently mature willow leaf horsewhip grass seeds grain of selection development, removal foreign peoples plant grain, clast, dust
Equal impurity；
(2) it soaks seed: being fitted into valve bag after the seed chosen is mixed with the potassium nitrate solution that concentration is 0.01%, and will
Valve bag is put into 5 DEG C of refrigerating box and refrigerates 36h；
(3) it cleans: seed is pulled out from potassium nitrate solution, and rinsed 1 time with clear water, the seed cleaned is picked and is attached to
In valve bag, and valve bag is put into -1 DEG C of household freezer and freezes 12h；
(4) it cultivates: the seed after freezing being taken out from valve bag, and uniformly spills into and is lined with 1 layer of wet filter paper
In culture dish；Then culture dish is put into vernalization in incubator；At 20 DEG C, incubation time is 8 days for the temperature control of incubator；
Then the seed to have germinateed is taken out from culture dish, then remaining non-chitting piece is put into 5 DEG C of refrigerating box, refrigeration 2
It；It is to cultivate vernalization 8 days in 20 DEG C of incubators that finally culture dish, which is placed again into from refrigerating box taking-up to temperature,.
The method of the invention is simple, it is economical, do not influenced by factors such as season, weather, natural calamities, to willow leaf horsewhip
The production practices of grass are of great significance.
Embodiment 2: a method of improving willow leaf Verbena officinalis percentage of seedgermination comprising following steps:
(1) choose seeds: normal, the sufficiently mature willow leaf horsewhip grass seeds grain of selection development, removal foreign peoples plant grain, clast, dust
Equal impurity；
(2) it soaks seed: being fitted into valve bag after the seed chosen is mixed with the potassium nitrate solution that concentration is 7%, and will be certainly
Envelope is put into 10 DEG C of refrigerating box and refrigerates 60h；
(3) it cleans: seed is pulled out from potassium nitrate solution, and rinsed 3 times with clear water, the seed cleaned is picked and is attached to
In valve bag, and valve bag is put into 1 DEG C of household freezer and freezes 36h；
(4) it cultivates: the seed after freezing is taken out from valve bag, and uniformly spill into and pad the wet filter paper that is of five storeys
In culture dish；Then culture dish is put into vernalization in incubator；The temperature control of incubator is at 30 DEG C, incubation time 13
It；Then the seed to have germinateed is taken out from culture dish, then remaining non-chitting piece is put into 10 DEG C of refrigerating box, it is cold
5 days, hiding；It is to cultivate vernalization 15 days in 30 DEG C of incubators that finally culture dish, which is placed again into from refrigerating box taking-up to temperature,.
The method of the invention is simple, it is economical, do not influenced by factors such as season, weather, natural calamities, to willow leaf horsewhip
The production practices of grass are of great significance.
Embodiment 3: a method of improving willow leaf Verbena officinalis percentage of seedgermination comprising following steps:
(1) choose seeds: normal, the sufficiently mature willow leaf horsewhip grass seeds grain of selection development, removal foreign peoples plant grain, clast, dust
Equal impurity；
(2) it soaks seed: being fitted into valve bag after the seed chosen is mixed with the potassium nitrate solution that concentration is 0.2%, and will
Valve bag is put into 7 DEG C of refrigerating box and refrigerates 48h；
(3) it cleans: seed is pulled out from potassium nitrate solution, and rinsed 2 times with clear water, the seed cleaned is picked and is attached to
In valve bag, and valve bag is put into 0 DEG C of household freezer and is freezed for 24 hours；
(4) it cultivates: the seed after freezing is taken out from valve bag, and uniformly spill into and pad the wet filter paper that haves three layers
In culture dish；Then culture dish is put into vernalization in incubator；The temperature control of incubator is at 25 DEG C, incubation time 10
It；Then the seed to have germinateed is taken out from culture dish, then remaining non-chitting piece is put into 7 DEG C of refrigerating box, it is cold
3 days, hiding；It is to cultivate vernalization 11 days in 25 DEG C of incubators that finally culture dish, which is placed again into from refrigerating box taking-up to temperature,.
The method of the invention is simple, it is economical, do not influenced by factors such as season, weather, natural calamities, to willow leaf horsewhip
The production practices of grass are of great significance.
Embodiment 4: a certain number of willow leaf horsewhip grass seeds are selected and are bisected into two groups, first group uses method of the invention
Handled, second group be placed directly in culture dish 20 DEG C -30 DEG C at a temperature of allow it to grow naturally；Two groups of willow leaf Verbena officinalis
Germination be as follows:
As can be seen from the table, conventional method is compared, willow leaf horsewhip grass seed can be made using the method described in the present invention
Germination percentage is increased to 85-95% from original 1%, while the period of germinateing shortens to 18-25 days for 30 days compared to original；Utilize this
Kind technology can solve the problems, such as that willow leaf Verbena officinalis seed germination rate is low and sprout time is longer.
It is the preferred embodiment of the present invention above, for those skilled in the art, is not departing from
Under the premise of the principle of the invention, several improvements and modifications can also be made, these improvements and modifications also should be regarded as guarantor of the invention
Protect range.
Claims (4)
1. it is a kind of improve willow leaf Verbena officinalis percentage of seedgermination method, which is characterized in that itself the following steps are included:
(1) choose seeds: normal, the sufficiently mature willow leaf horsewhip grass seeds grain of selection development, it is miscellaneous that removal foreign peoples plants grain, clast, dust etc.
Matter；
(2) soak seed: it is 5 that temperature is placed on after the seed chosen is mixed with the potassium nitrate solution that mass concentration is 0.01%-7%
36-60h is refrigerated in DEG C -10 DEG C of environment；
(3) it cleans: seed is pulled out from potassium nitrate solution, and rinsed 1-3 times with clear water, the seed cleaned is placed on temperature
To freeze 12h-36h in -1 DEG C -1 DEG C of environment；
(4) it cultivates: the seed after freezing is uniformly spilt into the culture dish for being lined with 1-5 layers of wet filter paper；It then will culture
Ware is put into the environment that temperature is 20 DEG C -30 DEG C and carries out vernalization.
2. a kind of method for improving willow leaf Verbena officinalis percentage of seedgermination according to claim 1, which is characterized in that step 4
The time of middle vernalization is 8-13 days.
3. a kind of method for improving willow leaf Verbena officinalis percentage of seedgermination according to claim 1 or 2, which is characterized in that will
The seed to have germinateed in step 4 takes out from culture dish, and remaining non-chitting piece is put into the environment that temperature is 5 DEG C -10 DEG C
In, it refrigerates 2-5 days；Then culture dish is placed again into the environment that temperature is 20 DEG C -30 DEG C and carries out vernalization.
4. a kind of method for improving willow leaf Verbena officinalis percentage of seedgermination according to claim 3, which is characterized in that vernalization
Time is 8-15 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811070038.0A CN109168408A (en) | 2018-09-13 | 2018-09-13 | Method for improving germination rate of vervain willow seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811070038.0A CN109168408A (en) | 2018-09-13 | 2018-09-13 | Method for improving germination rate of vervain willow seeds |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109168408A true CN109168408A (en) | 2019-01-11 |
Family
ID=64911116
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811070038.0A Pending CN109168408A (en) | 2018-09-13 | 2018-09-13 | Method for improving germination rate of vervain willow seeds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109168408A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113016257A (en) * | 2021-03-15 | 2021-06-25 | 内蒙古蒙草草种业有限公司 | Method for promoting germination of carnation seeds |
| CN116472965A (en) * | 2023-05-23 | 2023-07-25 | 贵州大学 | Tissue culture quick-breeding system for verbena salicifolia |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012149115A1 (en) * | 2011-04-26 | 2012-11-01 | International Horticultural Technologies, Llc. | Soil free planting composition |
| CN105850729A (en) * | 2016-04-06 | 2016-08-17 | 中喜生态产业股份有限公司 | Verbena bonariensis cultivation method |
-
2018
- 2018-09-13 CN CN201811070038.0A patent/CN109168408A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012149115A1 (en) * | 2011-04-26 | 2012-11-01 | International Horticultural Technologies, Llc. | Soil free planting composition |
| CN105850729A (en) * | 2016-04-06 | 2016-08-17 | 中喜生态产业股份有限公司 | Verbena bonariensis cultivation method |
Non-Patent Citations (3)
| Title |
|---|
| 刘峰等编著: "《种子检验学》", 31 October 2017, 西北农林大学出版社 * |
| 彭玉辅等: "赤霉素对柳叶马鞭草种子发芽及幼苗生长的影响", 《江西科学》 * |
| 李卫琼编著: "《园艺植物栽培技术》", 31 December 2013, 重庆：重庆大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113016257A (en) * | 2021-03-15 | 2021-06-25 | 内蒙古蒙草草种业有限公司 | Method for promoting germination of carnation seeds |
| CN116472965A (en) * | 2023-05-23 | 2023-07-25 | 贵州大学 | Tissue culture quick-breeding system for verbena salicifolia |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190111 |
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| RJ01 | Rejection of invention patent application after publication |