CN109122057A - The Spawn incubation bag production method and application of the fungi-proofing function of tool ventilation - Google Patents
The Spawn incubation bag production method and application of the fungi-proofing function of tool ventilation Download PDFInfo
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- CN109122057A CN109122057A CN201811025226.1A CN201811025226A CN109122057A CN 109122057 A CN109122057 A CN 109122057A CN 201811025226 A CN201811025226 A CN 201811025226A CN 109122057 A CN109122057 A CN 109122057A
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- 238000011534 incubation Methods 0.000 title claims abstract description 40
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 33
- 238000009423 ventilation Methods 0.000 title abstract description 10
- 241000894006 Bacteria Species 0.000 claims abstract description 66
- 239000004744 fabric Substances 0.000 claims abstract description 58
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 40
- 241000233866 Fungi Species 0.000 claims abstract description 24
- 230000035784 germination Effects 0.000 claims abstract description 15
- 239000004745 nonwoven fabric Substances 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 10
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 16
- 244000062793 Sorghum vulgare Species 0.000 claims description 12
- 239000001963 growth medium Substances 0.000 claims description 12
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- 230000001954 sterilising effect Effects 0.000 claims description 12
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- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 9
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 9
- 238000011081 inoculation Methods 0.000 claims description 9
- 235000009973 maize Nutrition 0.000 claims description 9
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- 235000011941 Tilia x europaea Nutrition 0.000 claims description 8
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 8
- 239000004571 lime Substances 0.000 claims description 8
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- 235000004439 Coprinus comatus Nutrition 0.000 claims description 7
- 241001313708 Dictyophora phalloidea Species 0.000 claims description 7
- 241000222684 Grifola Species 0.000 claims description 7
- 241000123107 Phellinus Species 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 7
- 235000006521 Pleurotus eryngii var ferulae Nutrition 0.000 claims description 6
- 244000088486 Pleurotus eryngii var. ferulae Species 0.000 claims description 6
- 239000004743 Polypropylene Substances 0.000 claims description 6
- 241000958510 Stropharia rugosoannulata Species 0.000 claims description 6
- 239000011159 matrix material Substances 0.000 claims description 6
- 235000012054 meals Nutrition 0.000 claims description 6
- 230000007935 neutral effect Effects 0.000 claims description 6
- 229920001155 polypropylene Polymers 0.000 claims description 6
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 6
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 6
- 229920000742 Cotton Polymers 0.000 claims description 5
- 239000004809 Teflon Substances 0.000 claims description 5
- 229920006362 Teflon® Polymers 0.000 claims description 5
- 241000209140 Triticum Species 0.000 claims description 5
- 235000021307 Triticum Nutrition 0.000 claims description 5
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- 238000007689 inspection Methods 0.000 claims description 5
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 4
- 239000003205 fragrance Substances 0.000 claims description 4
- 229910052602 gypsum Inorganic materials 0.000 claims description 4
- 239000010440 gypsum Substances 0.000 claims description 4
- 239000011505 plaster Substances 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 241000894007 species Species 0.000 claims description 3
- 239000010902 straw Substances 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 2
- 239000011148 porous material Substances 0.000 claims description 2
- 239000004575 stone Substances 0.000 claims description 2
- 241000222519 Agaricus bisporus Species 0.000 claims 2
- 229920006361 Polyflon Polymers 0.000 claims 1
- 239000006071 cream Substances 0.000 claims 1
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 229910002090 carbon oxide Inorganic materials 0.000 description 2
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
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- UBAZGMLMVVQSCD-UHFFFAOYSA-N carbon dioxide;molecular oxygen Chemical compound O=O.O=C=O UBAZGMLMVVQSCD-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/64—Cultivation containers; Lids therefor
- A01G18/66—Cultivation bags
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
The present invention relates to a kind of edible fungi strain bag production methods of the fungi-proofing function of tool ventilation, belong to edible mushroom technical field.The Spawn incubation bag is made of Spawn incubation bag body and fungi-proofing cloth patch；The fungi-proofing cloth patch is overlapped by non-woven fabrics with polytetrafluoroethylmicroporous microporous membrane, is determined behind aperture that 2 materials are repressed in a mold using ultrasonic wave and is formed, fungi-proofing cloth pastes aperture as 0.01~0.05um.Adopting said method can make the edible mushroom seed in container obtain sufficient clean air while growing bacterium germination, and fungi-proofing cloth pastes and can effectively exclude the various microorganisms in air, it is ensured that the strain of multiple eating bacterium kind is grown safely in a reservoir.
Description
One, technical field:
The present invention relates to the Spawn incubation bag production methods and application of the fungi-proofing function of tool ventilation, belong to fungus growing technique
Field.It include the specification requirement of strain packing container, in container fungi-proofing cloth patch quality and aperture specification requirement, bacterium culture medium
Composition and strain production process.The method can be such that the edible mushroom seed in container breathes while growing bacterium germination to clean
Net air effectively excludes the various microorganisms in air by aperture small in fungi-proofing cloth patch, it is ensured that strain is in a reservoir
Safety growth.
Two, background technique
Edible mushroom has high protein, low fat, contains a variety of amino acid of needed by human body and microelement, and have numerous food product
The health-care effect that can not replace, it has become 21 century health type food.As people's living standard improves, China is to edible
Bacterium consumption figure is with annual 7% speed sustainable growth.It has been reached in the annual consumption of the China for possessing 1,300,000,000 populations, edible mushroom
More than 3000 ten thousand tons.In addition edible mushroom is to be produced using agriculture and forestry organic waste material as culture medium, meets international community and is mentioned
3R (Reduce minimizing, Reuse are recycled, the Recycle recycling) index system advocated, is conducive to construct Agro-ecological System
Benign cycle, and promote ecological environment it is lasting, harmonious, develop in a healthy way.China is a large agricultural country, and agriculture and forestry crop is discarded
Resourceful, most of stalk material becomes environomental pollution source after burning, and stalk can also generate a large amount of two in spontaneous fermentation
Carbonoxide pollutes air.And cellulose and sugar in stalk material etc. can be decomposed and be absorbed to the mycelia of edible mushroom especially agaricus bisporus
Larger molecular organics matter converts stalk material to the food of fungi of high protein, makes major contribution for human survival and health, promotees
Into the healthy and sustainable development of rural economy, the two-win of environmental protection and economic development is realized.
Edible fungus culturing matrix nutrition is abundant, and especially culture medium quick-acting nutrition after high-temperature sterilization increases, and is meeting
Mycelia is also more subject to various miscellaneous bacterias and worm mite infringement while growth, China's mushroom industry is in the course of cultivation by various
The underproduction caused by disease pest endangers is up to 15~20%.The control polluted in growth program is the core of entire growth,
Annual strain enterprise has 10% or more strain to be infected and scrap by various insect pests.Although in each ring of growth
It is designed with pollution prevention program in section, requires from the selection of strain substrate container, matrix sterilizing, transfer room air cleaning to bacteria-producing room
Air cleaning require etc. make various Control Technologies, but have do not discharge place, disease pest pollution still has.Through producing
The careful investigation of program is analyzed, it is believed that the bacterium germination stage of the most of places of pollution source after inoculation, because the container of strain is all set
There is an inoculation mouth, the air filtration gasket hole in the bottle cap of inoculation mouth is excessive, is easy to bring disease pest source into air exchange
And cause strain pollution.At present China's edible fungus species manufacturing enterprise the process that original seed and cultivar produce use vial,
Plastic bottle, polybag make container, and bottleneck or sack are sealed with plastic closure, plus layer of non-woven fabric as empty in plastics upper shield
The barrier of gas filtering and barrier miscellaneous bacteria, but non-woven fabrics aperture, in 50~100um, the air hole of large aperture keeps air unobstructed, but
It is that miscellaneous bacteria and pest also free in and out therewith, therefore, frequent occurrence, acarid and bacterium mosquito also often appear in feeding in bag to living contaminants
Mycelia causes insect pest and miscellaneous bacteria to break out, and strain pollution, bacteria environmental degradation, a large amount of strains are scrapped when serious, and strain production stops
It rectifies or closes down.How to solve by the living contaminants that bottle/bag mouth imprecision causes to be that current edible fungus species industry faces prominent
It goes wrong, it is the important leverage of strain safety in production that searching, which can ventilate and the Novel antibacterial cloth of disease pest can be stopped to paste,.
Three, summary of the invention
Technical problem
It is high the technical problem to be solved by the present invention is to solve pollution rate present in existing edible fungus species production bacterium germination
The low problem of yield rate.A kind of tool through the invention ventilate fungi-proofing function edible fungi strain bag production and application, realize
The purpose of the high finished product rate of strain production.
Technical solution
A kind of tool of the invention ventilate fungi-proofing function edible fungi strain bag production method and application, comprising: fungi-proofing cloth
It pastes determining aperture and the patch strain bag production of fungi-proofing cloth and strain produces three steps.
The Spawn incubation bag is made of Spawn incubation bag body (1) and fungi-proofing cloth patch (2)；Spawn incubation bag body has on (1)
Sack (3), fungi-proofing cloth patch (2) are sticked to below sack (3) from the position 160~200mm of sealing part, while wiping out fungi-proofing cloth patch inside
Pocket portion, fungi-proofing cloth patch aperture is 0.01~0.05um.Fungi-proofing cloth pastes long 110mm × wide 60mm, and number cells reach
5600.
The fungi-proofing cloth patch is overlapped by non-woven fabrics with microporous teflon membran and is integrally suppressed in a mold, answers
Determine that aperture fungi-proofing cloth patch aperture is 0.01~0.05um with ultrasonic wave；The microporous teflon membran is by polytetrafluoroethylene (PTFE)
Resin is made, and membrane aperture is required in 0.01~0.05um, 50~80um of thickness, and for porosity up to 80%, the non-woven fabrics selects river
The ES hot rolling type non-woven fabrics of Su Changzhou Han Ke non-woven fabrics Co., Ltd production, with a thickness of 50g/m2。
The Spawn incubation bag body (1) is a kind of polybag for having transparent high temperature resistant high-pressure polypropylene CPP film, according to life
Produce the formula and matrix weight of parent species, strain and cultivar, respectively three kinds of bacterium bag specifications, parent species bacterium bag specification are as follows: long by 300 ×
Wide by 180 × thickness 0.07mm；Original seed bacterium bag specification: long 350 × wide by 220 × thickness 0.07mm；Cultivar bacterium bag specification are as follows: long by 450 ×
Wide by 300 × thickness 0.07mm.
The Spawn incubation bag of the method production can be in dictyophora phalloidea, coprinus comatus, Stropharia rugoso-annulata, agaricus bisporus, Brazilian mushroom, perfume (or spice)
It is applied in mushroom, Pleurotus ferulae, grifola frondosus and Phellinus Spawn incubation.
Factory formula used in the Spawn incubation bag has 2:
One are as follows: millet 85%, fermented maize core powder 7%, neutral calcium carbonate 5%, edible plaster 2%, lime 1% are used
In the strain of production straw rotting fungus kind, e.g., dictyophora phalloidea, coprinus comatus, Stropharia rugoso-annulata, agaricus bisporus and Brazilian mushroom；
Secondly are as follows: weedtree sawdust 52%, cotton seed hulls 15%, maize cob meal 15%, wheat skin 15%, neutral calcium carbonate 1%, food
With gypsum 1%, lime 1%, for producing the strain of domestomycetes kind, such as Phellinus, Pleurotus ferulae, grifola frondosus and mushroom.
Parent species bacterium bag charging weight is 0.8 kilogram；Original seed bacterium bag charging weight is 1.5 kilograms；Cultivar bacterium bag charging weight is 2.5
Kilogram.
Strain production comprises the concrete steps that: impregnating dry millet clear water by recipe requirements, picks up when moisture absorption 40%
Boiling is carried out, is pulled out while hot after boiling, shakeouts and cools down on ground, the calcium carbonate of recipe requirements, gypsum and lime and millet are stirred
It mixes uniformly, then maize cob meal and millet is stirred evenly, pack sterilizing when moisture reaches 41%-43% after measured.Sterilising temp
120 DEG C of 4 hours of holding are controlled, offers for sale when temperature drops to 60 DEG C, aseptically continues cool to 25 DEG C of when progress
Inoculation opens sack in 100 grades of air cleaning transfer rooms and accesses strain, closes sack immediately, and overturn strain in sack bag
It is uniformly mixed with culture medium, strain bag is placed in transfer room bacterium germination, be inoculated with bacterium germination temperature in 15 days back pkt.s and control 18~20
DEG C, strain is moved into 14~16 DEG C of bacterium germination inventories again after bacterium bag mycelia is up-to-standard on inspection and continue culture 15 days, to mycelia
Amount sends out full full bag, when bacterium bag is white, then bacterium bag is moved in 5 DEG C of libraries and receive Aquaponic 5~10 days, to culture medium in sack
White is presented, it is anhydrous to ooze now, it opens and is covered with mycelia on bag inspection grain, mushroom fragrance is dense, and Spawn incubation terminates, at low temperature
Preservation waits vanning to sell.
Beneficial effect
1, the present invention has done many screening tests, preferably high-pressure polypropylene CPP film conduct out in the selection of strain container
Strain plastic bag container.For this film by the production and sales of Zhejiang Zhong Chengsu industry Co., Ltd, the name of an article is that 5 layers of co-extrusion of high temperature resistant synthesize film,
This film can be packed into various edible fungi substrates (grain, cotton seed hulls, sawdust, corncob, wheat, fermentation material) as Spawn incubation bag,
Capacity is up to 0.5~10.0kg；This type of strain bag increases by 50% compared to the strain bag thickness of other types, but more soft than other strain bags
It is soft, sack can be repeatedly overturn after sealing and carries out shaking kind, and not brittle do not crack of the plastics of strain bag can still keep original shape；Bacterium
Kind bag transparency is high, and mycelia growth, miscellaneous bacteria in bag is clearly viewed in bag external enwergy and breeds situation；Plastics kind bag high temperature resistant can be in temperature
Spend for 5 hours above non-sclerous non-fusible in 125 DEG C of sterilizing cabinets, indices reach the ideal performance indicator of strain bag.
2, the present invention show that 4 data of table, test result are shown with 0.01~0.05um by multiple batches of screening test
The indices of the mushroom of the fungi-proofing cloth patch strain bag culture in aperture are normal, and aeration is preferable in bacterium bag, bacterium does not occur
Flatulence phenomenon in bag；Water-in-bag part is sufficient, and fungi-proofing cloth patch window culture medium moisture content is maintained, and mycelia growth is normal；Especially
It is that miscellaneous bacteria amount significantly reduces during cultivating in a fungus bag, seed yield rate is increased to 99.6%, stable product quality.By more than 2 years
Multiple batches of test determine using the fungi-proofing cloth in the aperture 0.01~0.05um patch as edible fungus species culture bag.According to edible fungi
Required air quantity in silk growth and set, the oxygen and carbon dioxide that can only be allowed in air are swapped by micropore, and are utilized
Micropore obstructs multiple-microorganism and enters.Its fungi-proofing cloth pastes long 110mm × wide 60mm, number cells up to more than 5600, be able to satisfy in
Oxygen exchange capacity needed for the mycelia of multiple eating bacterium strain grows.
3, the fungi-proofing cloth patch quality being fabricated to by polytetrafluoroethylene film is frivolous, and performance is stablized, and high temperature high voltage resistant is subjected to 125
DEG C 5 small timeinvariances of high temperature, micropore do not reduce, improve one toward it is used with low pressure polyethylene microporous barrier non-refractory and
The shortcomings that pore membrane reduces (micropore is made of polytetrafluoroethylene film in the prior art).Therefore, there is hole using this fungi-proofing cloth patch
Diameter constant magnitude, the strong advantage of fungi-proofing performance.
By a large amount of screening tests, the ES hot rolling type nonwoven for having selected changzhou Han Ke non-woven fabrics Co., Ltd to produce
Cloth, with a thickness of 50g/m2, such non-woven fabrics 5 hours under 125 DEG C high temperature it is non-fusible, indeformable, can and polypropylene plastics pocket
Fusion forms closed closing line, and ES hot rolling type non-woven fabrics has preferable gas permeability, water repellency and bullet drawing property.
4, fungi-proofing cloth patch strain bag is applied to make container 2 years more, production dictyophora phalloidea, coprinus comatus, Stropharia rugoso-annulata, agaricus bisporus,
The mycelia such as Brazilian mushroom, mushroom, Pleurotus ferulae, grifola frondosus and Phellinus grow more slow edible fungus variety and its strain production
In, it is shown from table 5, making container using fungi-proofing cloth patch strain bag has mycelia fast growing, and mycelia is not agglomerated in bag, and hand is grabbed
Fungus block is easily dispersed, and mushroom fragrance is dense, and incubation time is obviously shortened, and strain yield rate significantly improves.The mycelia purseful time shortens
By 30 days or so, 0.6% or less contamination rate；Mould contamination rate 0.3%；Yeast pollution rate 0%；Acarid growth rate 0%；Kind
Sub- yield rate is up to 99% or more.
Four, Detailed description of the invention
Fig. 1: parent species Spawn incubation bag structural schematic diagram
Fig. 2: original seed Spawn incubation bag structural schematic diagram
Fig. 3: cultivar original seed Spawn incubation bag structural schematic diagram
The fungi-proofing cloth of 1- Spawn incubation bag donor 2- pastes 3- Spawn incubation bag sack
Five, specific embodiment
The invention will be further described with reference to the accompanying drawing.
The Spawn incubation bag is made of Spawn incubation bag body (1) and fungi-proofing cloth patch (2)；Spawn incubation bag body has on (1)
Sack (3), fungi-proofing cloth patch (2) are sticked to below sack (3) from the position 160~200mm of sealing part, while wiping out fungi-proofing cloth patch inside
Pocket portion, fungi-proofing cloth patch aperture is 0.01~0.05um, and fungi-proofing cloth pastes long 110mm × wide 60mm, and number cells reach
5600.
Spawn incubation bag body (1) is a kind of polybag for having transparent high temperature resistant high-pressure polypropylene CPP film, female according to production
The formula and matrix weight of kind, strain and cultivar make 3 kinds of strain bag specifications, parent species bacterium bag specification respectively are as follows: long by 300 ×
Wide by 180 × thickness 0.07mm, charging weight are 0.8 kilogram；Original seed strain bag specification: long 350 × wide by 220 × thickness 0.07mm, charging weight
It is 1.5 kilograms；Cultivar strain bag specification are as follows: long 450 × wide by 300 × thickness 0.07mm, charging weight are 2.5 kilograms.
1, Spawn incubation bag body makes
The present invention has done many screening tests in the selection of strain container, from vial, plastic bottle to polybag all into
The charging production of hybrid seeds of having gone test, observes bacterium germination and living contaminants situation, it is intended to therefrom select the strain package for being adapted to growth
Packaging container, and cooperate to make the plastics kind and corresponding bacterium that the strain for being suitable for a variety of kinds produces with plastic products producer
Kind bag specification.It is shown in Table 1, is moulded by testing the high-pressure polypropylene CPP film preferably gone out from the strain container of 6 seed types as strain
Material bag container.This film is by the production and sales of Zhejiang Zhong Chengsu industry Co., Ltd, and the name of an article is that 5 layers of co-extrusion of high temperature resistant synthesize film, this film is made
For Spawn incubation bag, it can be packed into various edible fungi substrates (grain, cotton seed hulls, sawdust, corncob, wheat, fermentation material), capacity reaches
0.5~10.0kg；This type of strain bag increases by 50% compared to the strain bag thickness of other types, but more soft than other strain bags, can
It repeatedly overturns sack after sealing to carry out shaking kind, not brittle do not crack of the plastics of strain bag can still keep original shape；Strain bag
Transparency height is clearly viewed mycelia growth, miscellaneous bacteria in bag in bag external enwergy and breeds situation；Plastics kind bag high temperature resistant can be in temperature 125
For 5 hours above non-sclerous non-fusible in DEG C sterilizing cabinet, indices reach the ideal performance indicator of strain bag.
1 strain bottle/bag type of table is compared with performance
2, fungi-proofing cloth patch aperture determines and makes
The existing seed bottle in China or plastic bag for cultivation of mushroom are using bottleneck or sack as charging, inoculation, closing and to ventilate
(air exchange) is used, closes and ventilation is the both sides of conflict, therefore, be often able to aeration in practice and just care for
Not upper closure, vice versa, so the pollution of miscellaneous bacteria and pest in strain production happens occasionally.Preferably to solve sack
The contradiction of closing and ventilation, feed inlet and outlet and blow vent, which are provided separately, can efficiently solve ventilation and closed contradiction, that is, exist
A hole is opened by Air permenbility size in the designated position of polybag, then pastes when upper air hole is exclusively used in growth at aperture position
Ventilation needs, and plastic bag mouth is sealed with sealing machine immediately after charging inoculation, and the ventilation solved in growth in this way needs
The problem of disease pest is invaded from sack in growth is prevented again.The fungi-proofing cloth patch of growth in polybag is also to have wanting for profession
Ask, it not only needs to ventilate, be satisfied with growth needs in bag, but can not be excessive because of venthole or excessive due to in bag walking
Seed moisture content influences mycelia growth, while being also required to have aeration again and can stop a kind of dedicated fungi-proofing of multiple-microorganism
Gas cloth --- i.e. fungi-proofing cloth patch is as the Special ventilating film in strain bag.In order to by the germ often occurred in growth and evil
Through the patch filtering of fungi-proofing cloth outside bag, we search and than right Trichoderma virides, Pseudomonas thallus, bacillus subtilis worm
Gemma, yeast, tiny anhui fritillary and rhabditis axei ovum volume size data, and by these biology with mycelia grow institute
The big decimal of volume of the oxygen, carbon dioxide and hydrone that need is compared, and the results are shown in Table 2, it can be seen from the table oxygen molecule, two
Carbonoxide, hydrone volume be nanoscale, be respectively smaller than 1800 times of Pseudomonas, 3000 times of yeast, Trichoderma viride 4000
It again, is even more to be less than nematode and ten thousand times of acarid or more, to select the fungi-proofing cloth patch in suitable aperture to provide effective foundation.In this data
On the basis of selected from the fungi-proofing cloth in the aperture 0.01~0.05um patch strain bag the production of hybrid seeds carried out with 6 0.5 rank margins respectively
Bacterium germination test, temperature tolerance, aeration, flatulence, mushroom mycelium upgrowth situation, bacterial growth situation and the mould for observing bacterium bag are raw
6 correlation factors such as long situation obtain 4 data of table by multiple batches of screening test, test result shows with 0.01~
The indices of the mushroom of the fungi-proofing cloth patch strain bag culture in the aperture 0.05um are normal, and aeration is preferable in bacterium bag, does not have
There is the flatulence phenomenon of bacterium bag；Water-in-bag part is sufficient, and fungi-proofing cloth patch window culture medium moisture content is maintained, and mycelia growth is normal；
Especially miscellaneous bacteria amount significantly reduces during cultivating in a fungus bag, and kind bag yield rate is increased to 99.6%, stable product quality.Pass through 2
Multiple batches of test more than year is determined using the fungi-proofing cloth patch in the aperture 0.01~0.05um as edible fungus species growbag.
2 predominant gas of table, microorganism and pest volume difference in size
3 Pollution In Edible Mushroomss kind mycelium width of table
The fungi-proofing cloth of table 4 patch aperture performance compares
The fungi-proofing cloth patch is overlapped and integral by non-woven fabrics with microporous teflon membran, determines aperture using ultrasonic wave
2 materials are suppressed and integral, cut body of the non-woven fabrics as microporous teflon membran in a mold afterwards, make it poly- with high pressure
Ethylene pastes the ventilation window that strain bag is made.
3, fungi-proofing cloth patch strain bag production
This patent proposes that Spawn incubation bag is made of culture bag body and fungi-proofing cloth patch；There is sack in Spawn incubation bag body upper end,
Obtained fungi-proofing cloth patch is pasted onto below sack from the position 160~200mm of sealing part, while wiping out the bag of fungi-proofing cloth patch inside
Body parts of plastics is pasted using fungi-proofing cloth as the ventilative window of bacterium bag.Spawn incubation process is put with stand-type, therefore fungi-proofing cloth
It pastes position to exist without matrix, is conducive to air exchange.
4, the application of Spawn incubation bag
Productive culture based formulas used in Spawn incubation bag is 2 kinds: 1, millet 85%, fermented maize core powder 7%, carbon neutral
Sour calcium 5%, edible plaster 2%, lime 1%.This formula for producing straw rotting fungus kind, e.g., dictyophora phalloidea, coprinus comatus, Stropharia rugoso-annulata,
Agaricus bisporus and Brazilian mushroom；2, weedtree sawdust 52%, cotton seed hulls 15%, maize cob meal 15%, wheat skin 15%, neutral calcium carbonate
1%, edible plaster 1%, lime 1%.This formula is for producing domestomycetes kind, such as Phellinus, Pleurotus ferulae, grifola frondosus and mushroom
Deng.
Strain production process: before this being impregnated dry millet clear water by recipe requirements, is picked up when water absorption is up to 40%, into
Row steam boiling terminates for 50 minutes through 100 DEG C, shakeouts while hot cooling on ground；Again by the calcium carbonate of recipe requirements, gypsum and stone
Ash is stirred evenly with millet, is eventually adding maize cob meal and is stirred evenly with millet, moisture reaches the culture medium being stirred after measured
41~43%, it can pack into sterilizing cabinet and carry out autoclave sterilization；Sterilising temp controls 120 DEG C of 4 hours of holding, to temperature
Degree removes sterilizing cabinet when dropping to 60 DEG C, and strain bag is inoculated with when aseptically continuing cool to 25 DEG C；In 100 grades of skies
Sack is opened in gas purification inoculation room and accesses strain, closes sack immediately, and overturning sack mixes strain in bag with culture medium
Uniformly, strain bag is placed on bacteria-producing room bacterium germination, bacterium germination temperature controls 18~20 DEG C in 15 days after inoculation, and bacterium bag mycelia is on inspection
Strain is moved into 14~16 DEG C of bacterium germination libraries again after up-to-standard and continues culture 15 days, sends out full full bag to hyphae length, bacterium bag is in white
Color but water-in-bag, which divide, to be increased, then bacterium bag is moved in 5 DEG C of libraries and receive Aquaponic 5~10 days, whole strain mycelial growth time
It is 40~50 days, anhydrous to ooze now when culture medium presentation white in sack, a bag survey is opened, mycelia, bacterium in bag are covered on grain
Silk does not agglomerate, and hand is grabbed fungus block and easily dispersed, and mushroom fragrance is dense, indicates that the strain cultural hypha phase terminates, strain can be placed on low temperature
Lower preservation waits vanning to sell.
Carrying out edible fungi strain bag 2 years in dictyophora phalloidea, coprinus comatus, Stropharia rugoso-annulata, agaricus bisporus, Brazilian mushroom, mushroom, asafoetide more
The mycelia such as mushroom, grifola frondosus and Phellinus, which grow in more slow edible fungus variety and its strain production, is applied.Our company
The kind strain that a variety of mycelia slow growths of container production are done using fungi-proofing cloth patch strain bag, shows, mycelia is raw from table 5
Long quick, incubation time shortens obviously, and strain yield rate significantly improves.The mycelia purseful time 30 days or so, contamination rate
0.6% or less；Mould contamination rate 0.3%；Yeast pollution rate 0%；Acarid growth rate 0%；99% or more bag yield rate of kind.
The fungi-proofing cloth in the aperture 5 0.01~0.05um of table pastes strain bag application
Claims (7)
- The Spawn incubation bag production method of fungi-proofing function 1. a kind of tool is ventilated, it is characterised in that: the Spawn incubation bag is by strain It cultivates bag body (1) and fungi-proofing cloth patch (2) is constituted；Have in Spawn incubation bag body (1) sack (3), fungi-proofing cloth patch (2) is sticked to sack (3) lower section is from the position 160~200mm of sealing part, while wiping out the pocket portion of fungi-proofing cloth patch inside, and the fungi-proofing cloth pastes aperture For 0.01~0.05um, fungi-proofing cloth pastes long 110mm × wide 60mm, and number cells are up to 5600.
- 2. according to the method described in claim 1, it is characterized by: the fungi-proofing cloth patch is by non-woven fabrics and polytetrafluoroethylene (PTFE) micropore Film is overlapped and integrally suppresses in a mold, determines pore size using ultrasonic wave, fungi-proofing cloth patch aperture is determined as 0.01 ~0.05um；The microporous teflon membran is made of polyflon, and membrane aperture is required in 0.01~0.05um, 50~80um of thickness, for porosity up to 80%, the non-woven fabrics selects the ES heat of changzhou Han Ke non-woven fabrics Co., Ltd production Type non-woven fabrics is rolled, with a thickness of 50g/m2。
- 3. method according to claim 1 or 2, it is characterised in that:The Spawn incubation bag body (1) is that a kind of tool transparent high temperature resistant high-pressure polypropylene CPP film, that is, five-layer co-squeezing synthesizes film system The polybag of work, according to production parent species, the formula and matrix weight of strain and cultivar, respectively three kinds of bacterium bag specifications, parent species Bacterium bag specification are as follows: long 300 × wide by 180 × thickness 0.07mm；Original seed bacterium bag specification: long 350 × wide by 220 × thickness 0.07mm；Cultivar Bacterium bag specification are as follows: long 450 × wide by 300 × thickness 0.07mm.
- 4. the application of the Spawn incubation bag of one of claim 1-3 the method production.
- 5. application according to claim 4, which is characterized in that refer to Spawn incubation bag in culture dictyophora phalloidea, coprinus comatus, big ball Application in lid mushroom, agaricus bisporus, Brazilian mushroom, mushroom, Pleurotus ferulae, grifola frondosus and Phellinus strain.
- 6. application according to claim 4 or 5, which is characterized in that factory formula used in the Spawn incubation bag has 2 It is a,One are as follows: millet 85%, fermented maize core powder 7%, neutral calcium carbonate 5%, edible plaster 2%, lime 1%, for giving birth to The strain for producing straw rotting fungus kind, such as dictyophora phalloidea, coprinus comatus, Stropharia rugoso-annulata, agaricus bisporus and Brazilian mushroom；Secondly are as follows: weedtree sawdust 52%, cotton seed hulls 15%, maize cob meal 15%, wheat skin 15%, neutral calcium carbonate 1% eat stone Cream 1%, lime 1%, for producing the strain of domestomycetes kind, such as Phellinus, Pleurotus ferulae, grifola frondosus and mushroom；Parent species bacterium bag charging weight is 0.8 kilogram；Original seed bacterium bag charging weight is 1.5 kilograms；Cultivar bacterium bag charges weight as 2.5 public affairs Jin.
- 7. application according to claim 6, which is characterized in that impregnate dry millet clear water by recipe requirements, to moisture Pick up carry out boiling when being absorbed into 40%, pulled out while hot after boiling, shakeout it is cooling on ground, by the calcium carbonate required in formula, Gypsum and lime are stirred evenly with millet, then maize cob meal and millet are stirred evenly, when moisture is up to 41%~43% after measured Start pack sterilizing, sterilising temp controls 120 DEG C of 4 hours of holding, offers for sale when temperature drops to 60 DEG C, aseptically It is inoculated with when continuing cool to 25 DEG C, sack is opened in 100 grades of air cleaning transfer rooms and accesses strain, immediately sealed bag Mouthful, and overturning sack is uniformly mixed strain in bag with culture medium.Strain bag after being inoculated with is placed into transfer room bacterium germination, inoculation Bacterium germination temperature is controlled at 18~20 DEG C in 15 days afterwards, strain bag is moved into 14 again after bacterium bag mycelia is up-to-standard on inspection~ Continue culture 15 days in 16 DEG C of bacterium germination inventories, sends out full full bag to hyphae length, bacterium bag mycelia is white, then bacterium bag is moved to 5 DEG C of libraries It inside carries out receiving Aquaponic 5~10 days, white is presented to culture medium in sack, it is anhydrous to ooze now, it opens and is covered with bacterium on bag inspection grain Silk, mushroom fragrance is dense, and the Spawn incubation phase terminates, and preservation vanning can be waited to sell at low temperature.
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| CN201811025226.1A CN109122057A (en) | 2018-09-04 | 2018-09-04 | The Spawn incubation bag production method and application of the fungi-proofing function of tool ventilation |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811025226.1A CN109122057A (en) | 2018-09-04 | 2018-09-04 | The Spawn incubation bag production method and application of the fungi-proofing function of tool ventilation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110100650A (en) * | 2019-06-27 | 2019-08-09 | 贵州省园艺研究所(贵州省园艺工程技术研究中心) | A kind of method that Japanese red pine young pilose antler cultivar quickly produces |
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| CN110100650A (en) * | 2019-06-27 | 2019-08-09 | 贵州省园艺研究所(贵州省园艺工程技术研究中心) | A kind of method that Japanese red pine young pilose antler cultivar quickly produces |
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Application publication date: 20190104 |