CN109007846A - Compound packet of a kind of limiting amino acid nutrition and preparation method thereof - Google Patents
Compound packet of a kind of limiting amino acid nutrition and preparation method thereof Download PDFInfo
- Publication number
- CN109007846A CN109007846A CN201810634160.XA CN201810634160A CN109007846A CN 109007846 A CN109007846 A CN 109007846A CN 201810634160 A CN201810634160 A CN 201810634160A CN 109007846 A CN109007846 A CN 109007846A
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- China
- Prior art keywords
- amino acid
- preparation
- acid
- fermentation
- compound packet
- Prior art date
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Classifications
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- A—HUMAN NECESSITIES
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- A—HUMAN NECESSITIES
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- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
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Abstract
The invention belongs to the technical fields of fine chemistry industry, more particularly, to the compound packet of limiting amino acid nutrition and preparation method thereof for mixing compounding after a kind of multi-strain fermentation.Its quality percentage composition are as follows: lysine 10-50%, threonine 5-50%, tryptophan 5-30%, methionine 5%-25%, other amino acid 2-12%, mycoprotein content 0-10%, organic acid 0.5-2%, mineral matter element 0.1-3%, vitamin 0.1-3%, moisture 2-30%, the sum of each component mass percent are 100%.Preparation method predominantly obtains the fermentation liquid of lysine, threonine, tryptophan and methionine respectively, after mixing in proportion, obtains the corresponding compound packet of limiting amino acid nutrition by degerming, concentration, crystallization and baking process.The process that preparation method of the invention is simple, whole process is biology and physics, the environmental protection of product preparation process, safety.
Description
Technical field
The invention belongs to the technical fields of fine chemistry industry, more particularly, to the limitation for mixing compounding after a kind of multi-strain fermentation
Compound packet of acidic amino acid nutrition and preparation method thereof.
Background technique
In food and feed protein, one or more of essential amino acids lack or deficiency, will make body protein
Synthesis be restricted, since which limit the nutritive value of such protein, this amino acid is known as limiting amino acid.When
When certain amino acid far can not reach this ratio in certain food, even if protein content is high again, his advantage also can not be played,
This amino acid is exactly limiting amino acid.Limiting amino acid, which has, to be met human body and animal needs, promotes growth, improves ammonia
Base acid balance, the function of improving food utilization, save protein resource, add it to as that can substitute in meal expeller and mealsolvent
Fish meal to reduce feed cost, and can improve the quality of meat, improve lean meat percentage.Currently, there are no a kind of feeds in the market
Additive can be accomplished to add a variety of amino acid simultaneously.
Summary of the invention
To solve the above problems, multiple in view of the deficiencies of the prior art, the present invention provides being mixed after a kind of multi-strain fermentation
With the compound packet of limiting amino acid nutrition, solve each limiting amino acid type deficiency, ratio in food and feed additive
The problems such as unreasonable and compound process is cumbersome, and postprocessing working procedures are complicated.
The technical problem to be solved by the present invention is to what is be achieved through the following technical solutions, a kind of limiting amino acid nutrition is multiple
Close packet, quality percentage composition are as follows: lysine 10-50%, threonine 5-50%, tryptophan 5-30%, methionine 5%-25%,
Other amino acid 2-12%, mycoprotein content 0-10%, organic acid 0.5-2%, mineral matter element 0.1-3%, vitamin
0.1-3%, moisture 2-30%, the sum of each component mass percent are 100%.
Further, in the above-mentioned compound packet of acidic amino acid nutrition processed, other amino acid be arginine, ornithine,
Proline, glutamic acid, aspartic acid, glutamine, histidine, alanine, glycine, phenylalanine, valine, leucine and
At least one of isoleucine.The organic acid is at least one of succinic acid, malic acid, citric acid, lactic acid.It is described
Mineral matter element be at least one of potassium, sodium, iron, calcium, magnesium, aluminum and zinc.Above according to product demand, the amino of addition
Acid, organic acid and mineral matter element are important element and the trophic component of body tissue growth and development, are the auxiliary of a variety of Major Enzymes
The enzyme factor helps to adjust body metabolism, promotes the development and adjusting of human body and animal organ.
The present invention also provides the above-mentioned compound packet preparation method of limiting amino acid nutrition, steps are as follows: A, by lysine, Soviet Union
The strain of propylhomoserin, tryptophan and methionine by expansion cultivate corresponding propylhomoserin seed liquor, then seed liquor is put into hair respectively
Ferment in ferment culture medium corresponding propylhomoserin fermentation liquid；B, step A is obtained into lysine, threonine, tryptophan and egg ammonia respectively
The fermentation liquid of acid after mixing in proportion, obtains corresponding limiting amino acid by degerming, concentration, crystallization and baking process and seeks
Support compound packet；The strain of the step A is Corynebacterium glutamicum, the disconnected bacillus of yellow, corynebacterium ammoniagenes, stagnates bar bacterium, withered grass
At least one of strains such as bacillus and Escherichia coli.It is temperature 25-40 degree, pH6-7, culture that seed, which expands condition of culture,
26 times of dilution OD560nm to 0.28-0.55 of optical density.Propylhomoserin seed liquor is respectively connected to have gone out in the fermentation medium of bacterium and is sent out
Ferment ferments to the production acid value of respective concentration, stops fermentation, and fermentation process adds liquid glucose.The fermented and cultured of the propylhomoserin product
Temperature is 30-40 DEG C, pH6-7, and the period of fermented and cultured is 20-80h.
Corynebacterium glutamicum seed culture medium quality percentage composition is mainly glucose 2%, molasses yeast extract
0.1%, yeast plant nitrogen source 0.1%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.05%, pH6.5-7, surplus are pure water.
Escherichia coli seed culture medium quality percentage composition is mainly yeast extract 0.1%, peptone 0.1%, chlorination
Sodium 0.05%, pH6.5-7, surplus are pure water.
Brevibacterium flavum seed culture medium quality percentage composition is mainly glucose 2-8%, yeast extract 0.1%, sulphur
Sour ammonium 0.5%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.05%, pH6.5-7, surplus are pure water.
Corynebacterium ammoniagenes seed culture medium quality percentage composition is mainly glucose 2%, yeast plant nitrogen source 0.1%, egg
White peptone 0.2%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.05%, pH6.5-7, surplus are pure water.
Stagnating the quality percentage composition of bar bacterium seed culture medium is mainly glucose sugar 4%, yeast extract 0.1%, albumen
Peptone 0.1%, sodium chloride 0.05%, pH6.5-7, surplus are pure water.
Bacillus subtilis seed culture medium composition is mainly glucose 2-8%, yeast extract 0.1%, ammonium sulfate
0.5%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.05%, pH6.5-7, surplus are pure water.
Corynebacterium glutamicum fermentation medium quality percentage composition is mainly glucose 5-8%, double enzyme sugar 2%, molasses
0.5-1%, yeast extract 0.1%, yeast powder 0.1%, ammonium sulfate 0.2%, sodium dihydrogen phosphate 0.1%, disodium hydrogen phosphate
0.1%, magnesium sulfate 0.2%, methionine 0.005%, threonine 0.005%, pH6.5-7, surplus are pure water.
Escherichia coli fermentation culture medium quality percentage composition is mainly glucose 5%, yeast extract 0.1%, yeast powder
0.1%, ammonium sulfate 0.2%, potassium dihydrogen phosphate 0.1%, dipotassium hydrogen phosphate 0.1%, magnesium sulfate 0.2%, methionine 0.005%,
PH6.5-7, surplus are pure water.
Brevibacterium flavum fermentation medium quality percentage composition is mainly glucose 5%, yeast extract 0.1%, yeast
Powder 0.1%, corn starch 0.1%, ammonium sulfate 1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.2%, potassium chloride 0.2%,
PH6.5-7, surplus are pure water.
Corynebacterium ammoniagenes fermentation medium quality percentage composition is mainly glucose 2-8%, double enzyme sugar 2%, molasses 0.5-
1%, yeast extract 0.1%, yeast powder 0.1%, ammonium sulfate 0.2%, sodium dihydrogen phosphate 0.1%, disodium hydrogen phosphate 0.1%,
Potassium chloride 0.1%, magnesium sulfate 0.2%, pH6.5-7, surplus are pure water.
Stagnating the quality percentage composition of bacillus fermentation culture medium is mainly glucose 5%, yeast extract 0.1%, yeast powder
0.1%, ammonium sulfate 0.2%, potassium dihydrogen phosphate 0.1%, dipotassium hydrogen phosphate 0.1%, magnesium sulfate 0.2%, corn slurries 2%, taste
Smart 0.05%, methionine 0.005%, pH6.5-7, surplus is pure water.
Fermentation of bacillus subtilis culture medium quality percentage composition is mainly glucose 5%, double enzyme sugar 2%, corn starch
0.2%, yeast extract 0.1%, yeast powder 0.1%, amino acid powder 0.1%, ammonium sulfate 1%, potassium dihydrogen phosphate 0.1%, sulphur
Sour magnesium 0.2%, ferrous sulfate 0.05%, adenine 0.005%, pH6.5-7, surplus is pure water.
Compared with prior art, the compound packet of limiting amino acid nutrition of the invention is rich in lysine, threonine, tryptophan
And methionine, as several important essential amino acids and limiting amino acid, the dosage in terms of feed addictive continues
Increase, threonine is often added in the feed of teenage piglet and poultry, is that the second limiting amino acid of pannage and poultry are raised
The third limiting amino acid of material.And the nutritive value of phytoprotein and its feed can be improved in lysine, is conducive to develop albumen
Matter feed resource.Tryptophan due in the cereal such as corn, wheat, rice content it is seldom, due to amino acid have collaboration absorb
Effect, its shortage will also result in absorption of the animal to amino acid other in cereal.Preparation method of the invention is simple, entire mistake
One process of Cheng Junwei biology and physics, the environmental protection of product preparation process, safety.The food or feed obtained according to the present invention adds
Add agent ratio be 1:1-2:3-4:4-6, meet methionine in food nutrition and animal feed additive, tryptophan, threonine with
The gold blend proportion of lysine.In forage compounding, tryptophan and lysine, threonine are shared with rational proportion, to strengthen
Animal absorbs the collaboration of other monomers amino acid in cereal, improves absorption and utilization of the animal to feed nutrition.
Specific embodiment
Embodiment 1
A kind of compound packet of limiting amino acid nutrition of multi-strain fermentation preparation, the Corynebacterium glutamicum seed culture
Matrix amount percentage composition is mainly glucose 2%, molasses yeast extract 0.1%, yeast plant nitrogen source 0.1%, biphosphate
Potassium 0.05%, magnesium sulfate 0.05%, pH6.5-7；Escherichia coli seed culture medium quality percentage composition is mainly yeast extract
0.1%, peptone 0.1%, sodium chloride 0.05%, pH6.5-7；Brevibacterium flavum seed culture medium quality percentage composition is mainly
Glucose 2-8%, yeast extract 0.1%, ammonium sulfate 0.5%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.05%, pH6.5-
7；Corynebacterium ammoniagenes seed culture medium quality percentage composition is mainly glucose 2%, yeast plant nitrogen source 0.1%, peptone
0.2%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.05%, pH6.5-7；Stagnate the quality percentage composition of bar bacterium seed culture medium
Predominantly glucose sugar 4%, yeast extract 0.1%, peptone 0.1%, sodium chloride 0.05%, pH6.5-7；Bacillus subtilis
Seed culture medium quality percentage composition is mainly glucose 2-8%, yeast extract 0.1%, ammonium sulfate 0.5%, biphosphate
Potassium 0.05%, magnesium sulfate 0.05%, pH6.5-7；
Corynebacterium glutamicum fermentation medium quality percentage composition is mainly glucose 5-8%, double enzyme sugar 2%, molasses
0.5-1%, yeast extract 0.1%, yeast powder 0.1%, ammonium sulfate 0.2%, sodium dihydrogen phosphate 0.1%, disodium hydrogen phosphate
0.1%, magnesium sulfate 0.2%, methionine 0.005%, threonine 0.005%, pH6.5-7；Escherichia coli fermentation culture medium quality
Percentage composition is mainly glucose 5%, yeast extract 0.1%, yeast powder 0.1%, ammonium sulfate 0.2%, potassium dihydrogen phosphate
0.1%, dipotassium hydrogen phosphate 0.1%, magnesium sulfate 0.2%, methionine 0.005%, pH6.5-7；Brevibacterium flavum fermentation medium
Quality percentage composition is mainly glucose 5%, yeast extract 0.1%, yeast powder 0.1%, corn starch 0.1%, ammonium sulfate
1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.2%, potassium chloride 0.2%, pH6.5-7；Corynebacterium ammoniagenes fermentation medium quality
Percentage composition is mainly glucose 2-8%, double enzyme sugar 2%, molasses 0.5-1%, yeast extract 0.1%, yeast powder 0.1%,
Ammonium sulfate 0.2%, sodium dihydrogen phosphate 0.1%, disodium hydrogen phosphate 0.1%, potassium chloride 0.1%, magnesium sulfate 0.2%, pH6.5-7；
Stagnating the quality percentage composition of bacillus fermentation culture medium is mainly glucose 5%, yeast extract 0.1%, yeast powder 0.1%, sulphur
Sour ammonium 0.2%, potassium dihydrogen phosphate 0.1%, dipotassium hydrogen phosphate 0.1%, magnesium sulfate 0.2%, corn slurries 2%, monosodium glutamate 0.05%,
Methionine 0.005%, pH6.5-7；Fermentation of bacillus subtilis culture medium quality percentage composition is mainly glucose 5%, double enzymes
Sugared 2%, corn starch 0.2%, yeast extract 0.1%, yeast powder 0.1%, amino acid powder 0.1%, ammonium sulfate 1%, phosphoric acid
Potassium dihydrogen 0.1%, magnesium sulfate 0.2%, ferrous sulfate 0.05%, adenine 0.005%, pH6.5-7；Specific step is as follows:
(1) it carries out seed expansion and cultivates to obtain seed liquor, in 30-36 DEG C, pH6-6.5, cultivate 26 times of dilution OD560 of optical density
To 0.28-0.55；
(2) seed liquor of culture is respectively connected to have gone out in the fermentation medium of bacterium, respectively preparing lysine through fermentation, Soviet Union's ammonia
4 kinds of acid, tryptophan and methionine amino acid；
(3) according to different product demand, four kinds of amino acid zymotic fluids are mixed in proportion, by being removed including micro-filtration, sheet frame
Bacterium (not needing the step if corresponding product can contain bacterium), concentration, crystallization and baking process obtain corresponding restricted ammonia
The compound packet of base acid nutrition, the food obtained according to the present invention or feed addictive ratio are 1:1-2:3-4:4-6, meet food battalion
The gold blend proportion of methionine, tryptophan, threonine and lysine in feeding and animal feed additive.And then fermentation process is mended
The liquid glucose added is the glucose solution of concentration 50% (mass/volume), and stream plus glucose control fermentation liquid residual sugar are in 0.5-
1.2%.
(4) according to product form demand, lotion product and powder product can be prepared.
Embodiment 2
A kind of limiting amino acid nutrition compound packet of multi-strain fermentation preparation, obtain different aminoacids fermentation liquid it
Afterwards, can also be mixed by actual demand, such as in chicken feed, it is desirable that threonine and lysine in 1:1.2-2 ratio into
After row mixing, then prepare lotion or powder finished product respectively as required, the component including following mass percentage content: other amino
Acid: 2-12%, mycoprotein content 0-10%, organic acid 0.5-2%, mineral matter element 0.1-3%, vitamin 0.1-3%, water
Divide 2-30%, the sum of each component mass percent is 100%.
Embodiment 3
A kind of limiting amino acid nutrition compound packet of multi-strain fermentation preparation, obtain different aminoacids fermentation liquid it
Afterwards, mixed by actual demand, such as in beverage, it is desirable that press 1:1-2:3-4:4-6 methionine, tryptophan, threonine and rely
The golden ratio content of propylhomoserin is mixed, then prepares lotion or powder finished product, including following mass percent respectively as required
The component of content, other amino acid: 2-12%, mycoprotein content 0-10%, organic acid 0.5-2%, mineral matter element 0.1-
3%, vitamin 0.1-3%, moisture 2-30%, the sum of each component mass percent are 100%.
Embodiment 4
A kind of limiting amino acid nutrition compound packet of multi-strain fermentation preparation, obtain different aminoacids fermentation liquid it
Afterwards, it is mixed by actual demand, such as pregnant woman's type, children old man's type, raising memory type, Anti-fatigue type and liver-protecting and alcoholism-relieving
The functional aminos such as type beverage can be equal to methionine in 1:1:1-2:2-3: tryptophan: threonine: the ratio of lysine contains
Amount is mixed, then prepares lotion or powder finished product, the component including following mass percentage content, other ammonia respectively as required
Base acid: 2-12%, mycoprotein content 0-10%, organic acid 0.5-2%, mineral matter element 0.1-3%, vitamin 0.1-3%,
Moisture 2-30%, the sum of each component mass percent are 100%.
Obviously, the above embodiment of the present invention is only intended to clearly illustrate examples made by the present invention, and is not to this
The restriction of the embodiment of invention.It for those of ordinary skill in the art, on the basis of the above description can be with
It makes other variations or changes in different ways.There is no necessity and possibility to exhaust all the enbodiments.It is all in this hair
Made any modifications, equivalent replacements, and improvements etc., should be included in the claims in the present invention within bright spirit and principle
Within protection scope.
Claims (9)
1. a kind of compound packet of limiting amino acid nutrition, quality percentage composition are as follows: lysine 10-50%, threonine 5-50%,
Tryptophan 5-30%, methionine 5%-25%, other amino acid 2-12%, mycoprotein content 0-10%, organic acid 0.5-
2%, mineral matter element 0.1-3%, vitamin 0.1-3%, moisture 2-30%, the sum of each component mass percent are 100%.
2. the compound packet of limiting amino acid nutrition according to claim 1, it is characterised in that: other amino acid are
Arginine, ornithine, proline, glutamic acid, aspartic acid, glutamine, histidine, alanine, glycine, phenylalanine,
At least one of valine, leucine and isoleucine.
3. the compound packet of limiting amino acid nutrition according to claim 2, it is characterised in that: the organic acid is amber
At least one of acid, malic acid, citric acid, lactic acid.
4. the compound packet of limiting amino acid nutrition according to claim 3, it is characterised in that: the mineral matter element is
At least one of potassium, sodium, iron, calcium, magnesium, aluminum and zinc.
5. the compound packet preparation method of limiting amino acid nutrition, step described in a kind of any one of claim 1-4 are as follows:
A, by the strain of lysine, threonine, tryptophan and methionine by expansion cultivate corresponding propylhomoserin seed liquor, then will
Seed liquor be put into respectively ferment in fermentation medium corresponding propylhomoserin fermentation liquid；
B, step A is obtained to the fermentation liquid of lysine, threonine, tryptophan and methionine respectively, after mixing in proportion, by removing
Bacterium, concentration, crystallization and baking process obtain the corresponding compound packet of limiting amino acid nutrition.
6. preparation method according to claim 5, it is characterised in that: the strain of the step A is Corynebacterium glutamicum, Huang
Color break bacillus, corynebacterium ammoniagenes, at least one of stagnate the strains such as bar bacterium, bacillus subtilis and Escherichia coli.
7. preparation method according to claim 6, it is characterised in that: it is temperature 25-40 degree that seed, which expands condition of culture,
PH6-7 cultivates 26 times of dilution OD560nm to 0.28-0.55 of optical density.
8. preparation method according to claim 7, it is characterised in that: propylhomoserin seed liquor to be respectively connected to have gone out the fermentation of bacterium
It ferments in culture medium, ferments to the production acid value of respective concentration, stop fermentation, fermentation process adds liquid glucose.
9. preparation method according to claim 8, it is characterised in that: the fermented and cultured temperature of the amino acid products is
30-40 DEG C, pH6-7, the period of fermented and cultured is 20-80h.
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