CN109007804A - A kind of rose probiotics ferment and preparation method thereof - Google Patents
A kind of rose probiotics ferment and preparation method thereof Download PDFInfo
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- CN109007804A CN109007804A CN201810805178.1A CN201810805178A CN109007804A CN 109007804 A CN109007804 A CN 109007804A CN 201810805178 A CN201810805178 A CN 201810805178A CN 109007804 A CN109007804 A CN 109007804A
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- 238000000855 fermentation Methods 0.000 claims abstract description 102
- 230000004151 fermentation Effects 0.000 claims abstract description 87
- 241000220317 Rosa Species 0.000 claims abstract description 48
- 239000006041 probiotic Substances 0.000 claims abstract description 35
- 235000018291 probiotics Nutrition 0.000 claims abstract description 35
- 238000002360 preparation method Methods 0.000 claims abstract description 12
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 15
- 241000894006 Bacteria Species 0.000 claims description 12
- 102000004190 Enzymes Human genes 0.000 claims description 12
- 108090000790 Enzymes Proteins 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 11
- 230000001476 alcoholic effect Effects 0.000 claims description 10
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 10
- 238000002955 isolation Methods 0.000 claims description 6
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 5
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 5
- 241000186840 Lactobacillus fermentum Species 0.000 claims description 5
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 5
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 5
- 241000235342 Saccharomycetes Species 0.000 claims description 5
- 238000001514 detection method Methods 0.000 claims description 5
- 235000021552 granulated sugar Nutrition 0.000 claims description 5
- 239000004310 lactic acid Substances 0.000 claims description 5
- 235000014655 lactic acid Nutrition 0.000 claims description 5
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 5
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 5
- 239000002994 raw material Substances 0.000 abstract description 7
- 239000003963 antioxidant agent Substances 0.000 abstract description 6
- 235000006708 antioxidants Nutrition 0.000 abstract description 6
- 230000003078 antioxidant effect Effects 0.000 abstract description 5
- 239000011149 active material Substances 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 239000006210 lotion Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 11
- 238000011049 filling Methods 0.000 description 11
- 230000032683 aging Effects 0.000 description 10
- 238000004140 cleaning Methods 0.000 description 6
- 210000002966 serum Anatomy 0.000 description 6
- 238000004659 sterilization and disinfection Methods 0.000 description 6
- 239000000463 material Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
- 238000010171 animal model Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000003712 anti-aging effect Effects 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 206010000087 Abdominal pain upper Diseases 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 206010022998 Irritability Diseases 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 208000037093 Menstruation Disturbances Diseases 0.000 description 1
- 206010027339 Menstruation irregular Diseases 0.000 description 1
- 240000002657 Thymus vulgaris Species 0.000 description 1
- 235000007303 Thymus vulgaris Nutrition 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000003749 cleanliness Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 244000144993 groups of animals Species 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 238000010630 lipid peroxidation (MDA) assay Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000011022 operating instruction Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000012109 statistical procedure Methods 0.000 description 1
- 238000010254 subcutaneous injection Methods 0.000 description 1
- 239000007929 subcutaneous injection Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 239000001585 thymus vulgaris Substances 0.000 description 1
- 230000000451 tissue damage Effects 0.000 description 1
- 231100000827 tissue damage Toxicity 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000008736 traumatic injury Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention belongs to ferment production technical fields, and in particular to a kind of rose probiotics ferment and preparation method thereof.The present invention is using rose as primary raw material, under the fermentation of probiotics, the type, dosage of strict control probiotics and fermentation duration simultaneously, guarantee that the fermentation of raw material is more abundant, finally enable the nutriment in rose, active material, antioxidant to discharge to decomposite completely and, finally obtain it is a kind of with beautifying face and moistering lotion, anti-oxidant, senile-resistant efficacy rose probiotics ferment.
Description
Technical field
The invention belongs to ferment production technical fields, and in particular to a kind of rose probiotics ferment and preparation method thereof.
Background technique
Ferment, which refers to, ferments to fruit and vegetable by microorganism, a kind of liquid containing bioactive ingredients of extraction.Above-mentioned life
Object active constituent includes enzyme but is not limited to enzyme, including active antioxidants, biomass element etc., from fermentation participate in bacterium and
Food materials for fermentation.
The ferment rationally made, the active substance of plant extracted are from food materials, and participation bacterium of fermenting is even more people
The component part of body microecosystem.Therefore, the side effect of ferment is almost nil.Powerful effect, minimum side effect, in recent years
Come always in explosive increase trend.
Contain various chemical components in rose, such as the alcohol of fragrance, aldehyde, fatty acid, phenol and oil and rouge containing essence, rose
The flower and root just opened can be used as medicine, and have qi-regulating, promoting blood circulation, Thyme Extract, cure mainly irregular menstruation, traumatic injury, irritability stomachache, cream
The diseases such as too fat to move pain.Country's rose plant scale is more huge at present, and most of all to use as ornamental, flower loses after the florescence
It falls, causes and largely wasted with medicinal, edible value rose.
Rose it is existing it is further exploitation have rose paste (CN104000061A), rose enzyme (CN105852067A),
Rose (CN106190577A), but these products are not enough the extraction of nutriment ingredient in raw material.
Summary of the invention
In view of this, solving biography the purpose of the present invention is to provide a kind of rose probiotics ferment and preparation method thereof
The narrow problem of system rose application approach.
The technical scheme adopted by the invention is as follows:
A kind of preparation method of rose probiotics ferment, comprising the following steps:
Rose 900-950KG, brown granulated sugar 50-100kg are added in Zymolysis Equipment S1；
S2 continuously adds saccharomycete 5-10g and lactobacillus fermenti 10-15g into Zymolysis Equipment and carries out just fermentation, when fermentation
Between be 7-9 months；Lactobacillus plantarum 10-25g is just added after fermentation ferment for the second time, fermentation time 7-9 months；The
Lactobacillus acidophilus 5-10g, acetic acid bacteria 1-5g are added after secondary fermentation and continues third time fermentation 7-9 months；
After tri- stage fermentation of S3, detection pH stablizes between 2.7-3.6, and when alcoholic strength < 0.5%, fermentation is completed；
The fermentation liquid that fermentation is completed is filtered by S4；
S5 stand purifying: by filtered fermentation liquid stand purify, when alcoholic strength < 0.5%, lactic acid bacteria sum >=1 ×
106It purifies and completes when CFU/ml, obtain rose probiotics enzyme stoste.
When just fermentation, second of fermentation and third time are fermented in the S2, isolation air carries out anaerobic fermentation.
Zymolysis Equipment is fermenter.
Using rose probiotics ferment made from method made above.
The progress of rose probiotics enzyme stoste is filling to obtain finished product.
Filling condition: control Cleanliness reaches 100,000 grades of prescribed requirements, shop worker now washes your hands before entering workshop,
It changes one's clothes, sterilize, strictly operated by the operating instruction of bottle placer and Cover whirling Machine, the filling amount check at any time in filling progress,
The links such as seal quality, it is ensured that product complies with standard technical requirements.Before filling the container afterwards by equipment and pipeline carry out thoroughly cleaning and
Disinfection；It is filling to be bottled, bottle and bottle cap cleaning: bottle first uses pure water reverse-flush, then is dried up with clean compressed air, lid
It is carried out disinfection processing with disinfection cabinet, guarantee reaches sterility requirements.
It is preferred that following technical scheme: in S2, fermenting bucket is 850, and each 1200 liters of fermentation ladle capacity (adds every time
Enter raw material total amount 1000kg or so), electronic scale 2,1 ton of every fermenter charge, fermentation temperature is room temperature, and workshop sealing is protected
Cleaning, normal temperature fermentation are held, fermentation time is 24 months.
In S5, standing purifying tank is 50, and it is food grade plastic using material that each standing purifying tankage size, which is 30 tons,
Material.
When filling, the model TD-8 of bottle placer, the quantity of bottle placer is 2 sets；The model TD-SP1 of Cover whirling Machine, Cover whirling Machine
Quantity be 2 sets, filling speed be 2000 bottles/hour, bottle device be 30ml vial.
When bottle and bottle cap clean, bottle device 2 are managed；Full-automatic inverting bottle cleaning machine 2, the model of full-automatic inverting bottle cleaning machine
CP-15；Disinfection cabinet 1, the model ZTP-390 of disinfection cabinet.
Compared with prior art, the method have the benefit that:
The present invention is using rose as primary raw material, under the fermentation of probiotics, while the kind of strict control probiotics
Class, dosage and fermentation duration, guarantee that the fermentation of raw material is more abundant, fermentation process is divided into three phases and according to different phase
Characteristic be separately added into different strains, make fermentation have level it is more reasonable；The especially secondary fermentation stage, spontaneous fermentation,
It is not further added by sugar source, the nutriment in rose, active material, antioxidant is finally enabled to discharge decomposite completely
Come, finally obtains a kind of rose probiotics ferment with maintaining beauty and keeping young, antioxidant and anti-aging, increase rose probiotics
Ferment nutritive value.
Rose probiotics ferment produced by the present invention is free of any food additives, safe and healthy, has no toxic side effect, can put
The heart is enjoyed, and various people are suitable for；Solve that traditional rose application approach is narrow, product form Single-issue.
Specific embodiment
Illustrate a specific embodiment of the invention below with reference to embodiment, but following embodiment is used only to be described in detail
The present invention does not limit the scope of the invention in any way.
Raw material is added before fermenter through over cleaning, drying, ultraviolet disinfection, crushing in following embodiment.
A kind of embodiment 1: preparation method of rose probiotics ferment, comprising the following steps:
Rose 900kg, brown granulated sugar 100kg are added in fermenter S1；
S2 continuously adds saccharomycete 10g and lactobacillus fermenti 15g into fermenter and carries out just fermentation, ferments in fermentation process
Bucket overlay film, completely cuts off air, carries out anaerobic fermentation, and fermentation time is 8 months；Just after fermentation be added lactobacillus plantarum 25g into
Row ferments for the second time, fermenter overlay film in fermentation process, isolation air, progress anaerobic fermentation, and fermentation time 8 months；Second
Lactobacillus acidophilus 10g, acetic acid bacteria 5g are added after fermentation and continues third time fermentation, fermenter overlay film in fermentation process, isolation
Air, progress anaerobic fermentation, fermentation time 8 months；
After tri- stage fermentation of S3, detection pH stablizes between 2.7-3.6, and when alcoholic strength < 0.5%, fermentation is completed；
The fermentation liquid that fermentation is completed is filtered by S4；
S5 stand purifying: by filtered fermentation liquid stand purify, when alcoholic strength < 0.5%, lactic acid bacteria sum >=1 ×
106It purifies and completes when CFU/ml, obtain rose probiotics enzyme stoste.
A kind of embodiment 2: preparation method of rose probiotics ferment, comprising the following steps:
Rose 900kg, brown granulated sugar 50kg are added in fermenter S1；
S2 continuously adds saccharomycete 5g and lactobacillus fermenti 10g into fermenter and carries out just fermentation, ferments in fermentation process
Bucket overlay film, completely cuts off air, carries out anaerobic fermentation, and fermentation time is 9 months；Just after fermentation be added lactobacillus plantarum 10g into
Row ferments for the second time, fermenter overlay film in fermentation process, isolation air, progress anaerobic fermentation, and fermentation time 7 months；Second
Lactobacillus acidophilus 5g, acetic acid bacteria 1g are added after fermentation and continues third time fermentation, fermenter overlay film in fermentation process completely cuts off empty
Gas, progress anaerobic fermentation, fermentation time 7 months；
After tri- stage fermentation of S3, detection pH stablizes between 2.7-3.6, and when alcoholic strength < 0.5%, fermentation is completed；
The fermentation liquid that fermentation is completed is filtered by S4；
S5 stand purifying: by filtered fermentation liquid stand purify, when alcoholic strength < 0.5%, lactic acid bacteria sum >=1 ×
106It purifies and completes when CFU/ml, obtain rose probiotics enzyme stoste.
A kind of embodiment 3: preparation method of rose probiotics ferment, comprising the following steps:
Rose 930kg, brown granulated sugar 70kg are added in fermenter S1；
S2 continuously adds saccharomycete 8g and lactobacillus fermenti 12g into fermenter and carries out just fermentation, ferments in fermentation process
Bucket overlay film, completely cuts off air, carries out anaerobic fermentation, and fermentation time is 8 months；Just after fermentation be added lactobacillus plantarum 15g into
Row ferments for the second time, fermenter overlay film in fermentation process, isolation air, progress anaerobic fermentation, and fermentation time 8 months；Second
Lactobacillus acidophilus 8g, acetic acid bacteria 3g are added after fermentation and continues third time fermentation, fermenter overlay film in fermentation process completely cuts off empty
Gas, progress anaerobic fermentation, fermentation time 8 months；
After tri- stage fermentation of S3, detection pH stablizes between 2.7-3.6, and when alcoholic strength < 0.5%, fermentation is completed；
The fermentation liquid that fermentation is completed is filtered by S4；
S5 stand purifying: by filtered fermentation liquid stand purify, when alcoholic strength < 0.5%, lactic acid bacteria sum >=1 ×
106It purifies and completes when CFU/ml, obtain rose probiotics enzyme stoste.
After embodiment 1-3 obtains rose probiotics enzyme stoste, microbiological indicator, again need to be detected by national standard GB/T31121
Metal indicator is up to standard, other indexs reach professional standard T/CBFIA08003-2016, and then rose probiotics enzyme stoste is through special
It is piped to racking room, it is filling to obtain rose probiotics ferment finished product.
Effect experiment: rose probiotics ferment obtained by embodiment 1-3 influences reagent to mouse aging oxidation resistance
With experimental animal
SOD, GSH-Px, MDA assay kit, D- galactolipin；Healthy mice, weight (20 ± 2) g, male and female dual-purpose.
Grouping, aging model foundation and give sample
D- galactolipin causes aged animal model that can simulate Animal Aging process, can make the decline of SOD, GSH-PX activity, MDA
Content increases.
After mouse adaptive feeding 3d, it is randomly divided into 5 experiment groups, i.e. Normal group, aging model group, experimental group
1-3 group, every group 10.In addition to Normal group, remaining groups of animals every by body weight dose 150mg/kg subcutaneous injection D- half
Lactose solution, one time a day, Normal group inject same amount of normal saline.It is given respectively by weight for experimental group 1-3 group every simultaneously
Obtained rose probiotics ferment 5g/kg gives stomach-filling in embodiment 1-3, once a day, aging model group and normal control
Group gives same amount of normal saline stomach-filling, continuous 40d.
The measurement of SOD, GSH-PX, MDA content in serum
Blood is taken from mouse orbit, 37 DEG C of water-baths stand 30min, and 3000r/min is centrifuged 10min, serum are separated to obtain, referring to examination
The method and step of agent box specification measures the content of SOD, MDA, GSH-PX in serum respectively.
Statistical procedures
Experimental data is usedIt indicates, data, related data SPSS11.5 statistical software is handled using variance analysis
It is handled.
SOD, GSH-PX activity and MDA changes of contents situation in each group mice serum
Note: compared with Normal group,aP < 0.05；Compared with aging model group,bP < 0.05
By upper table the results show that aging model group is compared with Normal group, activity of SOD in serum is substantially reduced (P <
0.01), MDA content apparent increase (P < 0.05).For experimental group 1-3 group compared with aging model group, SOD content is significantly raised
(P < 0.05), MDA content are substantially reduced (P < 0.05).
D- galactolipin causes aged animal model that can simulate Animal Aging process, can make the decline of SOD, GSH-PX activity, MDA
Content increases.Because SOD can decompose oxygen radical, referred to as " street cleaner " of human body.GSH-PX relies primarily on its reduction, from
And play the role of that biological enzyme is protected to damage from peroxide.MDA be lipid oxidation decompose product, generate number can
To reflect degree of tissue damage indirectly.So measurement SOD, GSH-PX, MDA facilitate the case where understanding aging in body.This
The experimental results showed that rose probiotics ferment obtained by embodiment 1-3 can improve SOD, GSH-PX activity in serum and reduce
MDA content prompts rose probiotics ferment obtained by embodiment 1-3 to play a significant role in terms of anti-oxidant, anti-aging, and
It is the most significant with rose probiotics ferment effect obtained by embodiment 1.
Finally, it is stated that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, this field is common
Other modifications or equivalent replacement that technical staff makes technical solution of the present invention, without departing from technical solution of the present invention
Spirit and scope, be intended to be within the scope of the claims of the invention.
Claims (4)
1. a kind of preparation method of rose probiotics ferment, which comprises the following steps:
Rose 900-950KG, brown granulated sugar 50-100kg are added in Zymolysis Equipment S1；
S2 continuously adds saccharomycete 5-10g and lactobacillus fermenti 10-15g into Zymolysis Equipment and just ferment, and fermentation time is
7-9 months；Lactobacillus plantarum 10-25g is just added after fermentation ferment for the second time, fermentation time 7-9 months；Second
Lactobacillus acidophilus 5-10g, acetic acid bacteria 1-5g are added after fermentation and continues third time fermentation 7-9 months；
After tri- stage fermentation of S3, detection pH stablizes between 2.7-3.6, and when alcoholic strength < 0.5%, fermentation is completed；
The fermentation liquid that fermentation is completed is filtered by S4；
S5 stands purifying: filtered fermentation liquid being stood and is purified, as alcoholic strength < 0.5%, lactic acid bacteria sum >=1 × 106CFU/
It purifies and completes when ml, obtain rose probiotics enzyme stoste.
2. the preparation method of rose probiotics ferment according to claim 1, it is characterised in that: just fermentation, the in the S2
When secondary fermentation and third time are fermented, isolation air carries out anaerobic fermentation.
3. the preparation method of rose probiotics ferment according to claim 1, it is characterised in that: Zymolysis Equipment is fermentation
Bucket.
4. using rose probiotics ferment made from any one of claim 1-3 preparation method.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110801004A (en) * | 2019-11-15 | 2020-02-18 | 黑龙江惊哲森林食品集团有限公司 | Rose ferment powder for tonifying qi and blood and preparation method thereof |
| CN117223847A (en) * | 2022-12-08 | 2023-12-15 | 仁和全域(上海)大健康研究院有限公司 | Rose enzyme powder, solid beverage containing rose enzyme powder and preparation method thereof |
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| CN104643086A (en) * | 2015-03-11 | 2015-05-27 | 山东凤凰生物有限公司 | Preparation method of rose ferment |
| CN106578794A (en) * | 2016-12-01 | 2017-04-26 | 李恒 | Fermented fruit and vegetable juice beverage preparation process |
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2018
- 2018-07-20 CN CN201810805178.1A patent/CN109007804A/en active Pending
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|---|---|---|---|---|
| CN104643086A (en) * | 2015-03-11 | 2015-05-27 | 山东凤凰生物有限公司 | Preparation method of rose ferment |
| CN106578794A (en) * | 2016-12-01 | 2017-04-26 | 李恒 | Fermented fruit and vegetable juice beverage preparation process |
| CN107348272A (en) * | 2017-06-01 | 2017-11-17 | 扬生（南召）生物科技有限公司 | A kind of probiotics fruits and vegetables enzyme beverage and preparation method thereof |
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| CN110801004A (en) * | 2019-11-15 | 2020-02-18 | 黑龙江惊哲森林食品集团有限公司 | Rose ferment powder for tonifying qi and blood and preparation method thereof |
| CN117223847A (en) * | 2022-12-08 | 2023-12-15 | 仁和全域(上海)大健康研究院有限公司 | Rose enzyme powder, solid beverage containing rose enzyme powder and preparation method thereof |
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Address after: 474650 Taiwan Huayang Longsheng Science and Technology Industrial Park, Nanzhao County, Nanyang City, Henan Province Applicant after: HENGLIKANG BIOTECHNOLOGY Co.,Ltd. Address before: 474650 South Call Taiwan Huayang Longsheng Science and Technology Industrial Park, Nanyang City, Henan Province Applicant before: YANGSHENG (NANZHAO) BIOLOGICAL TECHNOLOGY Co.,Ltd. |
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Application publication date: 20181218 |