CN109007397A - A kind of fodder compound and preparation method thereof that can improve mouse heart morphosis and function - Google Patents
A kind of fodder compound and preparation method thereof that can improve mouse heart morphosis and function Download PDFInfo
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- CN109007397A CN109007397A CN201810964657.8A CN201810964657A CN109007397A CN 109007397 A CN109007397 A CN 109007397A CN 201810964657 A CN201810964657 A CN 201810964657A CN 109007397 A CN109007397 A CN 109007397A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/50—Feeding-stuffs specially adapted for particular animals for rodents
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention discloses a kind of fodder compound that can improve Omega3 and be overexpressed mouse heart morphosis and function, and the component of the fodder compound includes corn flour, wheat bran, dregs of beans, casein, carbohydrate, lysine, methionine, cystine, vegetable oil, animal fat, composite ore substance, mixed vitamin, cellulose.Omega3 of the present invention is overexpressed mouse feed composition and uses reasonable scientific formula; in the nutritional need for meeting Omega3 overexpression mouse growth; guarantee to eliminate the side effect for leading to cardiac shape structure and function damage after giving mild feed while its typical aspect of model; it is i.e. not damaged to heart under the premise of meeting Omega3 and being overexpressed mouse growth nutritional need and maintain its typical model feature, it lays a good foundation for accurate Study on Endogenous ω -3 PUFAs to the Protective effects of cardiovascular disease.Meanwhile the present invention also provides the preparation method that a kind of Omega3 is overexpressed mouse feed composition, the method is easy to operate, is suitable for industrialized production.
Description
Technical field
It is suitable for Omega3 overexpression mouse growth the present invention relates to experimental animal alimentary field, especially one kind and ties up
Hold typical model feature and to its cardiac shape structure and function fodder compound and preparation method thereof without side-effects.
Background technique
Currently, cardiovascular disease has become " number one killer " of global human.Omega-3 polyunsaturated fatty acids (ω-
3PUFAs) normal development to the mankind and other mammals and maintenance health are of crucial importance, and human cardiovascular disease's
Effect outstanding is played in prevention and treatment.But omega-3 polyunsaturated fatty acids cannot synthesize in most animals body,
It can only be supplemented from food.Scientist adds ω -3PUFAs in human diet and has done many researchs at present, obtains significant
Effect much has become health care product even drug by wide clinical application rich in ω -3PUFAs product, but exogenous addition
There are still many problems, such as the interference of diet structure, environment Confounding Factor by ω -3PUFAs.Therefore, needing to find one kind can be with
The method of the external factor such as diet structure interference is excluded to study ω -3PUFAs to the preventive and therapeutic effect and its machine of cardiovascular disease
System.
Omega3, which is overexpressed mouse, to convert ω -3PUFAs for ω -6PUFAs extra in vivo, to make the group of animal
ω -3PUFAs is knitted and is rich in organ, and the ω -6/ ω -3PUFAs ratio in tissue tends to be balanced.Omega3 crosses table at present
Up to mouse because by the interference of the external factor such as diet structure, not having become global scholar and studying ω -3PUFAs or ω -3/ ω -
Ideal animals model of the 6PUFAs ratio to cardiovascular disease protective effect.But Omega3, which is overexpressed mouse, does not have dedicated feed
It feeds, researcher gives mild feed (fat containing 10% or so) and carries out scientific research at present, but gives mild feed
After the phenomenon that causing cardiac shape structure and function to change cause researcher to pay high attention to, if heart chamber obviously expands, this is lacked
It falls into and brings certain limitation to Protective effects of the Study on Endogenous ω -3PUFAs to heart.It would therefore be highly desirable to develop
While one kind not only having met Omega3 overexpression mouse nutritional need and maintained the aspect of model, but also to the mouse heart morphosis
The fodder compound without side-effects with function.
Summary of the invention
A kind of suitable Omega3 overexpression is provided it is an object of the invention to overcome above-mentioned the deficiencies in the prior art place
Mouse growth simultaneously maintains the aspect of model and to cardiac shape structure and function fodder compound without side-effects.
Another object of the present invention also resides in offer and is suitble to Omega3 to be overexpressed mouse growth and maintains the aspect of model and right
The preparation method of cardiac shape structure and function fodder compound without side-effects, the preparation method is easy to operate, is suitable for industry
Metaplasia produces.
To achieve the above object, the technical scheme adopted by the invention is as follows: one kind can improve Omega3 be overexpressed mouse core
The fodder compound of dirty morphosis, the component of the fodder compound include corn flour, wheat bran, dregs of beans, casein, carbohydrate,
Lysine, methionine, cystine, vegetable oil, animal fat, composite ore substance, mixed vitamin and cellulose.
As the fodder compound of the present invention that can improve Omega3 and be overexpressed mouse heart morphosis and function
Embodiment, in the fodder compound parts by weight of each component be respectively as follows: 31~32 parts of corn flour, 7~8 parts of wheat bran,
8.5~10 parts of dregs of beans, 4~6 parts of casein, 8~11 parts of carbohydrate, 11~13 parts of lysine, 5~6 parts of methionine, cystine 2~3
Part, 3~4.5 parts of vegetable oil, 2~4 parts of animal fat, 3~5 parts of composite ore substance, 0.8~1.5 part of mixed vitamin, cellulose
4~5 parts.
As the fodder compound of the present invention that can improve Omega3 and be overexpressed mouse heart morphosis and function
Preferred embodiment, in the fodder compound parts by weight of each component be respectively as follows: 31.4 parts of corn flour, 7.6 parts of wheat bran,
9 parts of dregs of beans, 10 parts of carbohydrate, 12 parts of lysine, 5.6 parts of methionine, 2.4 parts of cystine, 3.4 parts of vegetable oil, moves 5 parts of casein
2.6 parts of object grease, 5 parts of composite ore substance, 1.2 parts of mixed vitamin, 4 parts of cellulose.
The preferred embodiment of mouse feed composition is overexpressed as Omega3 of the present invention, the vegetable oil is jade
At least one of rice bran oil, olive oil, peanut oil, rapeseed oil, linseed oil.
The preferred embodiment of mouse feed composition is overexpressed as Omega3 of the present invention, the animal fat is
At least one of lard, butter, fish oil.
The preferred embodiment of mouse feed composition, the composite ore substance are overexpressed as Omega3 of the present invention
Including calcium bicarbonate, sodium chloride, ferric sulfate, zinc sulfate, potassium iodide and copper sulphate.
The preferred embodiment of the special fodder compound of mouse, the mixing dimension life are overexpressed as Omega3 of the present invention
Element includes vitamin A, vitamin D, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B12, salt
Acid, calcium pantothenate, folic acid and biotin.
The preferred embodiment of mouse feed composition is overexpressed as Omega3 of the present invention, the cellulose is carboxylic
At least one of methylcellulose, cellulose polyethylene, lignin.
The preferred embodiment of mouse feed composition, the corn flour, bran are overexpressed as Omega3 of the present invention
The partial size of skin and dregs of beans is 80~100 mesh.
The preferred embodiment of small fodder compound is overexpressed as Omega3 of the present invention, the carbohydrate is lactose.
In addition, the present invention also provides the preparation method that a kind of Omega3 as described above is overexpressed mouse feed composition,
Described method includes following steps:
(1) it premixes: by casein, carbohydrate, lysine, methionine, cystine, composite ore substance, mixed vitamin and fibre
Dimension element is premixed, and premix is obtained；
(2) mixing: above-mentioned premix is uniformly mixed with corn flour, wheat bran, dregs of beans, add vegetable oil, animal oil with
And water, it is uniformly mixed, obtains mixture；
(3) it pelletizes: the mixture is made by particle using granulator；
(4) it dries: the particle is placed in drying in oven；
(5) it sterilizes: the finished product feedstuff that sterilizes to obtain will be carried out after drying particle packing.
The preferred embodiment of the preparation method of mouse feed composition is overexpressed as Omega3 of the present invention, it is described
Corn, wheat bran and dregs of beans pulverizer is first thrown into respectively before step (1) premix to crush, it is spare after crossing 80~100 meshes.
The preferred embodiment of the preparation method of mouse feed composition is overexpressed as Omega3 of the present invention, it is described
In step (3), the partial size of particle is 10mm~12mm, length 25mm.
The preferred embodiment of the preparation method of mouse feed composition is overexpressed as Omega3 of the present invention, it is described
In step (4), the temperature of baking oven is 70 DEG C~85 DEG C.
The preferred embodiment of the preparation method of mouse feed composition is overexpressed as Omega3 of the present invention, it is described
In step (5), sterilized using the irradiation of 20kGy 60Co- gamma-rays.
The preferred embodiment of the preparation method of mouse feed composition is overexpressed as Omega3 of the present invention, it is described
Method further includes the detection of step (6) finished product feedstuff: finished product made from step (5) being carried out nutritional ingredient and sanitary index is examined
It surveys, guarantees that the indices of finished product feedstuff comply with the national standard requirements.The finished product is subjected to nutritional ingredient and sanitary index inspection
It surveys, guarantees that the indices of finished product feedstuff meet mouse nutrient formulation standard (GB 14924.3-2001) and experimental animal nutrition
It is formulated universal qualities and requires (GB 14924.1-2001).
It is overexpressed the preferred embodiment of the preparation method of mouse feed composition as Omega3 of the present invention, is making
Before standby, first all raw materials are carried out with the detection of nutrition and sanitary index, so that shown Omega3 is overexpressed mouse feed composition
In the indexs such as microorganism, content of beary metal, the pesticide residue of each component meet experimental animal nutrient formulation sanitary standard (GB
14924.2-2001).
Omega3 of the present invention is overexpressed mouse feed composition, is prepared using the component of the special formulation,
Recipe ingredient, form and the smell that Omega3 of the present invention is overexpressed mouse feed composition are suitble to the habit of rodent
And hobby.Omega3 of the invention is overexpressed mouse feed composition by scientific formula, is overexpressed mouse meeting Omega3
The nutritional need of growth guarantees to eliminate while its typical aspect of model and gives mild feed (containing 10% left side in feed
Right fat) afterwards caused by the side effect that changes of cardiac shape structure and function, be accurate Study on Endogenous ω -3PUFAs to the heart
The Protective effects of vascular diseases are laid a good foundation.Moreover, Omega3 of the present invention is overexpressed mouse feed composition,
Nutrition is balanced, maintain the aspect of model and it is without side-effects to heart, without inorganic matter (including heavy metal), pesticide residual contamination and pole
The phytoestrogen of low content prevents animal after feeding from function change (such as oxidative stress index, blood lipids index) occur, thus
Exclude research of the phytoestrogen interference endogenous ω -3PUFAs to cardiovascular protective effect mechanism.
Detailed description of the invention
Fig. 1 is the comparison figure of the typical open in-heart operation under pulsating of each group mouse (M type).
Fig. 2 is each group mouse left ventricular contraction/diastolic dimensions (LVID；S and LVID；D) comparison figure.
Fig. 3 is the comparison figure of each group mouse heart left ventricular ejection fraction (EF) and shortening fraction (FS).
Fig. 4 is the comparison figure of each group mice serum CK and LDH content.
Fig. 5 is the comparison figure of each group mouse heart tissue cTnT and cTnI content.
Specific embodiment
To better illustrate the purpose of the present invention, technical scheme and beneficial effects, below in conjunction with attached drawing and specific implementation
The invention will be further described for example.
Embodiment 1
Omega3 of the present invention is overexpressed a kind of embodiment of mouse feed composition, and Omega3 described in the present embodiment is overexpressed
Mouse feed composition contains the following parts by weight: 31 parts of corn flour, 8 parts of wheat bran, 9.2 parts of dregs of beans, 6 parts of casein, sugar
11 parts of class, 5.2 parts of methionine, 3 parts of cystine, 3 parts of vegetable oil, animal fat 4,3 parts of composite ore substance, is mixed 11 parts of lysine
Close 1.5 parts of vitamin, 5 parts of cellulose.
In the present embodiment, the carbohydrate is lactose；The vegetable oil is corn oil；The animal fat is lard；It is described
Composite ore substance includes calcium bicarbonate, sodium chloride, ferric sulfate, zinc sulfate, potassium iodide and copper sulphate；The mixed vitamin includes
Vitamin A, vitamin D, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B12, hydrochloric acid, pantothenic acid
Calcium, folic acid and biotin；The cellulose is carboxymethyl cellulose.
Omega3 described in the present embodiment is overexpressed mouse feed composition and is prepared with the following method:
(1) it crushes: corn, wheat bran, dregs of beans being thrown into pulverizer respectively and crushed, cross 80~100 mesh, it is spare；
(2) it premixes: by casein, carbohydrate, lysine, methionine, cystine, composite ore substance, mixed vitamin and fibre
Dimension element is premixed, and premix is obtained；
(3) mixing: above-mentioned premix is uniformly mixed with corn flour, wheat bran, dregs of beans, add vegetable oil, animal oil with
And water, it is uniformly mixed, obtains mixture；
(4) it pelletizes: the mixture is made by particle, partial size 10mm~12mm, length 25mm using granulator；
(5) it dries: the particle is placed in 70 DEG C~85 DEG C of drying in oven；
(6) it sterilizes: using 20kGy 60Co- gamma-rays radiation sterilization after drying particle is dispensed with constant weight；
(7) detection of finished product feedstuff: finished product made from step (6) is subjected to nutritional ingredient and sanitary index detects, is guaranteed
The indices of finished product feedstuff comply with the national standard requirements.
Embodiment 2
Omega3 of the present invention is overexpressed a kind of embodiment of mouse feed composition, and Omega3 described in the present embodiment is overexpressed
Mouse feed composition contains the following parts by weight: 31.2 parts of corn flour, 7.8 parts of wheat bran, 9.5 parts of dregs of beans, casein 5.5
Part, 10.5 parts of carbohydrate, 11.5 parts of lysine, 5.4 parts of methionine, 2.8 parts of cystine, 3.8 parts of vegetable oil, 3.5 parts of animal fat,
3.5 parts of composite ore substance, 1.4 parts of mixed vitamin, 4.8 parts of cellulose.
In the present embodiment, the carbohydrate is lactose；The vegetable oil is olive oil and peanut oil；The animal fat is ox
Oil；The composite ore substance includes calcium bicarbonate, sodium chloride, ferric sulfate, zinc sulfate, potassium iodide and copper sulphate；The mixing dimension
Raw element includes vitamin A, vitamin D, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B12, salt
Acid, calcium pantothenate, folic acid and biotin；The cellulose is cellulose polyethylene.
Omega3 described in the present embodiment is overexpressed mouse feed composition and is prepared with the following method:
(1) it crushes: corn, wheat bran, dregs of beans being thrown into pulverizer respectively and crushed, cross 80~100 mesh, it is spare；
(2) it premixes: by casein, carbohydrate, lysine, methionine, cystine, composite ore substance, mixed vitamin and fibre
Dimension element is premixed, and premix is obtained；
(3) mixing: above-mentioned premix is uniformly mixed with corn flour, wheat bran, dregs of beans, add vegetable oil, animal oil with
And water, it is uniformly mixed, obtains mixture；
(4) it pelletizes: the mixture is made by particle, partial size 10mm~12mm, length 25mm using granulator；
(5) it dries: the particle is placed in 70 DEG C~85 DEG C of drying in oven；
(6) it sterilizes: using 20kGy 60Co- gamma-rays radiation sterilization after drying particle is dispensed with constant weight；
(7) detection of finished product feedstuff: finished product made from step (6) is subjected to nutritional ingredient and sanitary index detects, is guaranteed
The indices of finished product feedstuff comply with the national standard requirements.
Embodiment 3
Omega3 of the present invention is overexpressed a kind of embodiment of mouse feed composition, and Omega3 described in the present embodiment is overexpressed
Mouse feed composition contains the following parts by weight: 31.4 parts of corn flour, 7.6 parts of wheat bran, 9 parts of dregs of beans, 5 parts of casein,
10 parts of carbohydrate, 12 parts of lysine, 5.6 parts of methionine, 2.4 parts of cystine, 3.4 parts of vegetable oil, 2.6 parts of animal fat, composite ore
5 parts of substance, 1.2 parts of mixed vitamin, 4 parts of cellulose.
In the present embodiment, the carbohydrate is lactose；The vegetable oil is corn oil；The animal fat is lard；It is described
Composite ore substance includes calcium bicarbonate, sodium chloride, ferric sulfate, zinc sulfate, potassium iodide and copper sulphate；The mixed vitamin includes
Vitamin A, vitamin D, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B12, hydrochloric acid, pantothenic acid
Calcium, folic acid and biotin；The cellulose is lignin.
Omega3 described in the present embodiment is overexpressed mouse feed composition and is prepared with the following method:
(1) it crushes: corn, wheat bran, dregs of beans being thrown into pulverizer respectively and crushed, cross 80~100 mesh, it is spare；
(2) it premixes: by casein, carbohydrate, lysine, methionine, cystine, composite ore substance, mixed vitamin and fibre
Dimension element is premixed, and premix is obtained；
(3) mixing: above-mentioned premix is uniformly mixed with corn flour, wheat bran, dregs of beans, add vegetable oil, animal oil with
And water, it is uniformly mixed, obtains mixture；
(4) it pelletizes: the mixture is made by particle, partial size 10mm~12mm, length 25mm using granulator；
(5) it dries: the particle is placed in 70 DEG C~85 DEG C of drying in oven；
(6) it sterilizes: using 20kGy 60Co- gamma-rays radiation sterilization after drying particle is dispensed with constant weight；
(7) detection of finished product feedstuff: finished product made from step (6) is subjected to nutritional ingredient and sanitary index detects, is guaranteed
The indices of finished product feedstuff comply with the national standard requirements.
Embodiment 4
Omega3 of the present invention is overexpressed a kind of embodiment of mouse feed composition, and Omega3 described in the present embodiment is overexpressed
Mouse feed composition contains the following parts by weight: 31.5 parts of corn flour, 7.4 parts of wheat bran, 10 parts of dregs of beans, casein 4.5
Part, 9 parts of carbohydrate, 12.5 parts of lysine, 6 parts of methionine, 2.4 parts of cystine, 4 parts of vegetable oil, 3 parts of animal fat, mixed mineral
4 parts of matter, 1 part of mixed vitamin, 4.6 parts of cellulose.
In the present embodiment, the carbohydrate is lactose；The vegetable oil is rapeseed oil and linseed oil；The animal fat is pig
Oil and butter；The composite ore substance includes calcium bicarbonate, sodium chloride, ferric sulfate, zinc sulfate, potassium iodide and copper sulphate；It is described
Mixed vitamin includes vitamin A, vitamin D, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, dimension life
Plain B12, hydrochloric acid, calcium pantothenate, folic acid and biotin；The cellulose is lignin and carboxymethyl cellulose.
Omega3 described in the present embodiment is overexpressed mouse feed composition and is prepared with the following method:
(1) it premixes: by casein, carbohydrate, lysine, methionine, cystine, composite ore substance, mixed vitamin and fibre
Dimension element is premixed, and premix is obtained；
(2) mixing: above-mentioned premix is uniformly mixed with corn flour, wheat bran, dregs of beans, add vegetable oil, animal oil with
And water, it is uniformly mixed, obtains mixture；
(3) it pelletizes: the mixture is made by particle, partial size 10mm~12mm, length 25mm using granulator；
(4) it dries: the particle is placed in 70 DEG C~85 DEG C of drying in oven；
(6) it sterilizes: using 20kGy 60Co- gamma-rays radiation sterilization after drying particle is dispensed with constant weight；
(7) detection of finished product feedstuff: finished product made from step (6) is subjected to nutritional ingredient and sanitary index detects, is guaranteed
The indices of finished product feedstuff comply with the national standard requirements.
Embodiment 5
Omega3 of the present invention is overexpressed a kind of embodiment of mouse feed composition, and Omega3 described in the present embodiment is overexpressed
Mouse feed composition contains the following parts by weight: 31.8 parts of corn flour, 7.2 parts of wheat bran, 8.5 parts of dregs of beans, casein 4
Part, is mixed 8.5 parts of carbohydrate, 13 parts of lysine, 5.8 parts of methionine, 2.2 parts of cystine, 4.2 parts of vegetable oil, 2.6 parts of animal fat
Close 5 parts of minerals, 0.9 part of mixed vitamin, 4.2 parts of cellulose.
In the present embodiment, the carbohydrate is lactose；The vegetable oil is peanut oil and corn oil；The animal fat is pig
Oil and butter；The composite ore substance includes calcium bicarbonate, sodium chloride, ferric sulfate, zinc sulfate, potassium iodide and copper sulphate；It is described
Mixed vitamin includes vitamin A, vitamin D, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, dimension life
Plain B12, hydrochloric acid, calcium pantothenate, folic acid and biotin；The cellulose is carboxymethyl cellulose and cellulose polyethylene.
Omega3 described in the present embodiment is overexpressed mouse feed composition and is prepared with the following method:
(1) it crushes: corn, wheat bran, dregs of beans being thrown into pulverizer respectively and crushed, cross 80~100 mesh, it is spare；
(2) it premixes: by casein, carbohydrate, lysine, methionine, cystine, composite ore substance, mixed vitamin and fibre
Dimension element is premixed, and premix is obtained；
(3) mixing: above-mentioned premix is uniformly mixed with corn flour, wheat bran, dregs of beans, add vegetable oil, animal oil with
And water, it is uniformly mixed, obtains mixture；
(4) it pelletizes: the mixture is made by particle, partial size 10mm~12mm, length 25mm using granulator；
(5) it dries: the particle is placed in 70 DEG C~85 DEG C of drying in oven；
(6) it sterilizes: using 20kGy 60Co- gamma-rays radiation sterilization after drying particle is dispensed with constant weight；
(7) detection of finished product feedstuff: finished product made from step (6) is subjected to nutritional ingredient and sanitary index detects, is guaranteed
The indices of finished product feedstuff comply with the national standard requirements.
Embodiment 6
Omega3 of the present invention is overexpressed a kind of embodiment of mouse feed composition, and Omega3 described in the present embodiment is overexpressed
Mouse feed composition contains the following parts by weight: 32 parts of corn flour, 7 parts of wheat bran, 9 parts of dregs of beans, 5 parts of casein, carbohydrate 8
Part, 12 parts of lysine, 5.6 parts of methionine, 2 parts of cystine, 4.5 parts of vegetable oil, 2 parts of animal fat, 4.5 parts of composite ore substance,
0.8 part of mixed vitamin, 4.5 parts of cellulose.
In the present embodiment, the carbohydrate is lactose；The vegetable oil is rapeseed oil；The animal fat is butter；It is described
Composite ore substance includes calcium bicarbonate, sodium chloride, ferric sulfate, zinc sulfate, potassium iodide and copper sulphate；The mixed vitamin includes
Vitamin A, vitamin D, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B12, hydrochloric acid, pantothenic acid
Calcium, folic acid and biotin；The cellulose is lignin.
Omega3 described in the present embodiment is overexpressed mouse feed composition and is prepared with the following method:
(1) it crushes: to granular or block stock, throwing into pulverizer respectively such as corn, wheat bran, dregs of beans and crush, cross 80~100
Mesh, it is spare；
(2) it premixes: by casein, carbohydrate, lysine, methionine, cystine, composite ore substance, mixed vitamin and fibre
Dimension element is premixed, and premix is obtained；
(3) mixing: above-mentioned premix is uniformly mixed with corn flour, wheat bran, dregs of beans, add vegetable oil, animal oil with
And water, it is uniformly mixed, obtains mixture；Preferably, solid grease heating is added after melting completely；
(4) it pelletizes: the mixture is made by particle, partial size 10mm~12mm, length 25mm using granulator；
(5) it dries: the particle is placed in 70 DEG C~85 DEG C of drying in oven；
(6) it sterilizes: using 20kGy 60Co- gamma-rays radiation sterilization after drying particle is dispensed with constant weight；
(7) detection of finished product feedstuff: finished product made from step (6) is subjected to nutritional ingredient and sanitary index detects, is guaranteed
The indices of finished product feedstuff comply with the national standard requirements.
The effect test of the Omega3 of the present invention of embodiment 7 overexpression mouse feed composition
The present embodiment is mainly that Omega3 of the present invention is overexpressed the fodder compound of mouse and the fodder compound of comparative example
Performance comparison, the fodder compound of comparative example using Omega3 be overexpressed a kind of most general feed of mouse (mild feed,
Grease containing 10%).The fodder compound of comparative example contains the component of following parts by weight: 36.75 parts of cornstarch, casein
20 parts, 13.2 parts of maltodextrin, 10 parts of sucrose, 10 parts of soya-bean oil, 5.0 parts of cellulose, 3.5 parts of composite ore substance, mixing dimension life
Plain 1.0 parts, 0.3 part of cystine, 0.25 part of choline chloride.
1, experimental method
Experimental animal grouping and feeding management: SPF grades of Omega3 of 56 8 week old are overexpressed mouse and are randomly divided into experiment contrast
(fodder compound of the present invention is fed for group (fodder compound described in comparative example is fed, and experimental comparison group is set as) and experimental group
Support, be set as experimental group 1, experimental group 2, experimental group 3, experimental group 4, experimental group 5 and experimental group 6), every group 8.88 week old SPF
Grade C57BL/6J mouse is set as blank control group (conventional criteria forage feed, be set as blank control group), and the test period is 8 weeks.
The above experimental animal is provided by Experimental Animals Supervising Station, Guangdong Prov., and tissue sampling is dynamic in Experimental Animals Supervising Station, Guangdong Prov.'s barrier
[SYXK (Guangdong) 2012-0122] is carried out in object Experimental Establishment.Animal feeding environment temperature is 20 ± 2 DEG C, and humidity is 60 ± 2%,
Lighting hours is 12 ± 3h.Entire test process meets Guangdong Province's experimental animal feeding facility standard to the raising and disposition of animal
With experimental animal 3R principle.
Detection method: after above-mentioned mouse is fed 8 weeks, 1) contained using fatty acid in gas chromatography analysis heart tissue
Amount.Heart tissue 30mg is taken, after extracting its fatty acid using methyl esterization, upper machine testing.Testing result uses area normalization
Method calculates a certain content of fatty acid in heart tissue, analyzes the content of fatty acid in different grouping mouse heart tissue；2) heart
Dirty Ultrasonic tomography detection.Using Vevo2100 toy super-resolution power ultrasonic image system, is popped one's head in, obtained using MS-550D
After taking clearly parasternal short axis Type B image, under M type image, open in-heart operation under pulsating parameter is acquired, assesses mouse heart shape
State structure and heart function；3) biomarker analyte detection.After fasting 12h, take a blood sample through orbital venous plexus, after being statically placed in 4 DEG C of refrigerator 1h,
3000r/min is centrifuged 10min, takes supernatant, obtains serum, detects serum total cholesterol using automatic clinical chemistry analyzer
(TC), total triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), serum
Creatine kinase (CK) and lactic dehydrogenase (LDH) content；In addition, be euthanized after mouse acquisition blood, Quick Acquisition heart, in ice
Rinsed in cold physiological saline, remove blood, filter paper wipe it is dry, weighing, suitable ice-cold normal saline is added, is homogenized using tissue
Instrument is homogenized heart (Homogenization time 10s/ times.It is spaced 30s, continuous 3~5 times, is carried out in ice water), the 10% of preparation is homogenized with low
Warm low speed centrifuge 3000r/min is centrifuged 15min, obtains supernatant, detects mouse heart using luminex liquid-phase chip technology
Troponin (cTnI and cTnT) content in tissue.
2, statistical analysis technique
All experimental datas are for statistical analysis using 5 software of GraphPad Prism, and all data are used It indicates.Comparison among groups are examined using t, and p < 0.05 indicates significant difference, and p < 0.01 indicates extremely significant property
Difference.
3, interpretation of result
The content that polyunsaturated fatty acid in each group mouse heart tissue is detected by gas chromatograph, the results are shown in Table 1.With
Blank control group compares, and DHA content is significantly higher than blank control group in experimental comparison group and experimental group (1-5) mouse heart tissue
In (p < 0.05), experimental comparison group and experimental group (1-3/5-6) mouse heart tissue LA content be substantially reduced (p < 0.05orp <
0.01), AA content is remarkably decreased (p < 0.05) in experimental group (1-3/5-6) mouse heart tissue.In addition, experimental comparison group and reality
It tests ω -3/ ω -6PUFAs ratio in group (1-6) mouse heart tissue and significantly improves (p < 0.05).And experimental comparison group and experiment
In group (1-6) mouse heart tissue EPA, DHA, DPA, LA, AA and ω -3/ ω -6PUFAs ratio without significant difference (p >
0.05).The above result shows that fodder compound of the invention is able to maintain the typical model feature that Omega3 is overexpressed mouse.
Content of fatty acid (%) in 1 each group mouse heart tissue of table
Note: LA: linolenic acid, AA: arachidonic acid, EPA: eicosapentaenoic acid, DPA: clupanodonic acid；DHA: two
Dodecahexaene acid.Compared with blank control group, * * indicates p < 0.01；* p < 0.05 is indicated.
By Fig. 1 and Fig. 2 it is found that experimental comparison group mouse left ventricular contraction/diastolic dimensions (LVID；S and LVID；d)
Significantly greater than blank control group (p < 0.05), and (p < 0.05) is obviously reduced in experimental group (1-6) mouse left ventricular internal diameter, with blank
Control group is similar.Ejection fraction (EF) and shortening fraction (FS) are the main indicators for evaluating heart function, wherein EF is that reaction is left
Myocardium of ventricle fiber shortens function, and FS is reaction stress-shortening relationship.From the figure 3, it may be seen that experimental comparison group mouse EF and FS is small
In blank control group, and experimental group (1-6) mouse heart function is obviously improved, and EF and FS are above test control group, and between group
Indifference.The above result shows that Omega3 be overexpressed mouse give comparative example fodder compound feeding after cardiac shape structure with
Function changes, and gives fodder compound feeding of the present invention and can obviously improve this phenomenon, maintains mouse heart form knot
Structure and heart function it is normal.
As shown in Table 2, the horizontal no significant difference of each group mice serum TG, TC, LDL-C and HDL-C (p > 0.05), does not have
Statistical significance.But as the biochemical marker CK and LDH of auxiliary diagnosis myocardial damage, in experimental comparison group mice serum its
Content is significantly higher than blank control group (p < 0.05), and its content is substantially less than experiment contrast in experimental group (1-6) mice serum
Group (p < 0.05), and close to blank control group level, see Fig. 4.Meanwhile troponin T (cTnT) and troponin (cTnI) have
There is high Cardiac-specific, troponin has become that myocardial damage is most specific, most sensitive cardiac molecular marker at present
One of.As shown in Figure 5, troponin T (cTnT) and troponin (cTnI) content are bright in experimental comparison group mouse heart tissue
It is aobvious to be lower than blank control group, and its content is significantly raised in experimental group (1-6) mouse heart tissue, and with blank control group numerical value
It is close.Result above shows that Omega3 is overexpressed mouse and gives cardiac molecular markers after the feeding of comparative example fodder compound
It significantly changes, heart is prompted to be likely to occur a degree of damage, and give heart after fodder compound feeding of the present invention
Molecular marked compound, which has no, to be substantially change, and heart is prompted not occur exception, and the result is corresponding with ultrasonic experiments, is further proved
Fodder compound of the present invention is without side-effects to mouse body and heart, hence it is evident that is better than comparative example fodder compound.
2 each group mice serum TC, TG, HDL-C and LDL-C of table are horizontal
Show that Omega3 of the present invention is overexpressed mouse feed composition and can maintain Omega3 based on the above results
It is overexpressed mouse typical model feature, and can be obviously improved or eliminate mild feed causes Omega3 to be overexpressed mouse heart
The damage of morphosis and heart function can especially improve the widened morphological change of the chambers of the heart.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than protects to the present invention
The limitation of range is protected, although the invention is described in detail with reference to the preferred embodiments, those skilled in the art should
Understand, it can be with modification or equivalent replacement of the technical solution of the present invention are made, without departing from the essence of technical solution of the present invention
And range.
Claims (15)
1. one kind can improve the fodder compound that Omega3 is overexpressed mouse heart morphosis and function, which is characterized in that
The fodder compound includes corn flour, wheat bran, dregs of beans, casein, carbohydrate, lysine, methionine, cystine, vegetable oil, moves
Object grease, composite ore substance, mixed vitamin and cellulose.
2. the feed combinations that Omega3 is overexpressed mouse heart morphosis and function can be improved as described in claim 1
Object, which is characterized in that the parts by weight of each component are respectively as follows: 31~32 parts of corn flour, wheat bran 7~8 in the fodder compound
Part, 8.5~10 parts of dregs of beans, 4~6 parts of casein, 8~11 parts of carbohydrate, 11~13 parts of lysine, 5~6 parts of methionine, cystine 2
~3 parts, 3~4.5 parts of vegetable oil, 2~4 parts of animal fat, 3~5 parts of composite ore substance, 0.8~1.5 part of mixed vitamin, fibre
4~5 parts of dimension element.
3. the feed combinations that Omega3 is overexpressed mouse heart morphosis and function can be improved as claimed in claim 2
Object, which is characterized in that in the fodder compound parts by weight of each component be respectively as follows: 31.4 parts of corn flour, 7.6 parts of wheat bran,
9 parts of dregs of beans, 10 parts of carbohydrate, 12 parts of lysine, 5.6 parts of methionine, 2.4 parts of cystine, 3.4 parts of vegetable oil, moves 5 parts of casein
2.6 parts of object grease, 5 parts of composite ore substance, 1.2 parts of mixed vitamin, 4 parts of cellulose.
4. the Omega3 as claimed in any one of claims 1 to 3 that can improve is overexpressed mouse heart morphosis and function
Fodder compound, which is characterized in that the vegetable oil be corn oil, olive oil, peanut oil, rapeseed oil, in linseed oil at least
It is a kind of.
5. the Omega3 as claimed in any one of claims 1 to 3 that can improve is overexpressed mouse heart morphosis and function
Fodder compound, which is characterized in that the animal fat is at least one of lard, butter, fish oil.
6. the Omega3 as claimed in any one of claims 1 to 3 that can improve is overexpressed mouse heart morphosis and function
Fodder compound, which is characterized in that the composite ore substance includes calcium bicarbonate, sodium chloride, ferric sulfate, zinc sulfate, potassium iodide
And copper sulphate.
7. the Omega3 as claimed in any one of claims 1 to 3 that can improve is overexpressed mouse heart morphosis and function
Fodder compound, which is characterized in that the mixed vitamin includes vitamin A, vitamin D, vitamin E, vitamin K, dimension life
Plain B1, vitamin B2, vitamin B6, vitamin B12, hydrochloric acid, calcium pantothenate, folic acid and biotin.
8. the Omega3 as claimed in any one of claims 1 to 3 that can improve is overexpressed mouse heart morphosis and function
Fodder compound, which is characterized in that the cellulose is carboxymethyl cellulose, cellulose polyethylene, at least one in lignin
Kind.
9. the Omega3 as claimed in any one of claims 1 to 3 that can improve is overexpressed mouse heart morphosis and function
Fodder compound, which is characterized in that the partial size of the corn flour, wheat bran and dregs of beans is 80~100 mesh.
10. a kind of described in any item Omega3 that can improve of claims 1 to 3 are overexpressed mouse heart morphosis and function
Fodder compound preparation method, which is characterized in that described method includes following steps:
(1) it premixes: by casein, carbohydrate, lysine, methionine, cystine, composite ore substance, mixed vitamin and cellulose
It is premixed, obtains premix；
(2) mixing: above-mentioned premix being uniformly mixed with corn flour, wheat bran, dregs of beans, adds vegetable oil, animal oil and water,
It is uniformly mixed, obtains mixture；
(3) it pelletizes: the mixture is made by particle using granulator；
(4) it dries: the particle is placed in drying in oven；
(5) it sterilizes: the finished product feedstuff that sterilizes to obtain will be carried out after drying particle packing.
11. the feed combinations that Omega3 is overexpressed mouse heart morphosis and function can be improved as claimed in claim 10
The preparation method of object, which is characterized in that before the step (1) premix first by corn, wheat bran and dregs of beans throw into respectively pulverizer into
Row crushes, spare after crossing 80~100 meshes.
12. the feed combinations that Omega3 is overexpressed mouse heart morphosis and function can be improved as claimed in claim 10
The preparation method of object, which is characterized in that in the step (3), the partial size of particle is 10mm~12mm, length 25mm.
13. the feed combinations that Omega3 is overexpressed mouse heart morphosis and function can be improved as claimed in claim 10
The preparation method of object, which is characterized in that in the step (4), the temperature of baking oven is 70 DEG C~85 DEG C.
14. the feed combinations that Omega3 is overexpressed mouse heart morphosis and function can be improved as claimed in claim 10
The preparation method of object, which is characterized in that in the step (5), sterilized using the irradiation of 20kGy 60Co- gamma-rays.
15. the feed combinations that Omega3 is overexpressed mouse heart morphosis and function can be improved as claimed in claim 10
The preparation method of object, which is characterized in that further include the detection of step (6) finished product feedstuff: finished product made from step (5) is sought
It forms point and sanitary index detection, guarantees that the indices of finished product feedstuff comply with the national standard requirements.
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Cited By (2)
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