CN109006163A - A kind of Ganoderma tsugae cultivar cultural method, culture medium and culture medium preparation method - Google Patents

Abstract

The invention belongs to the technical field of artificial cultivation of Ganoderma lucidum, and in particular relates to a method for cultivating pine and fir Ganoderma lucidum cultivars, a culture medium and a preparation method for the culture medium. According to the mass percentage, the culture medium includes 25-30% of sawdust, 35-40% of cottonseed husk, bran Skin 15‑20%, corn cob 8‑12%, cornmeal 2‑5%, gypsum 1‑3%, superphosphate 0.5‑1.5%, lime 0.5‑1.5%, the total water content of the above ingredients is 60‑70%, And add 0.5-0.7g sodium selenite per kilogram of culture medium. The invention can make the spruce ganoderma lucidum fruit bodies compact in texture, good in quality, and neatly produced, so that the pines, fir and ganoderma lucidum can increase the yield and harvest.

Description

A method for cultivating pine, fir and ganoderma lucidum cultivars, a culture medium and a preparation method for the culture medium

technical field

The invention belongs to the technical field of ganoderma lucidum artificial cultivation, and in particular relates to a method for cultivating pine fir ganoderma cultivars, a culture medium and a preparation method for the culture medium.

Background technique

Pine fir Ganoderma lucidum, also known as Pine fir tree lucidum, Hemlock tree lucidum, and Wood Ganoderma lucidum, belongs to the Ganoderma lucidum genus. It is a unique species of Ganoderma lucidum in the forest regions of Northeast my country. It is an extremely rare and precious herbal medicine on the cut roots and decayed wood of fir, larch, red pine, and spruce in mixed forests. It is mainly distributed in the cold temperate zone of my country (produced in the Changbai Mountain area). Fruiting bodies annual, corky to woody, stalked. Cap reniform, semicircular or nearly fan-shaped, cap surface reddish brown to purple red, with paint-like luster, cap margin thin or blunt, sometimes involuted. The flesh is light white, white to off-white, and the mycelium is colorless to light brown. The mushroom meat is juicy when fresh and light when dry. The stipe is thick and short, with a purple-black surface and a paint-like luster. The boundary between the stipe and the cap is unclear, often horizontal and obtuse. Spores ovoid, often truncate at the apex. At present, wild pine, fir and Ganoderma lucidum resources are limited in quantity, and are becoming less and less. Studies have shown that Ganoderma lucidum is rich in nutrients, containing essential amino acids, nucleosides, trace elements, vitamins, bioactive polysaccharides, triterpenoids, alkaloids, etc., and is also a good source of dietary fiber necessary for maintaining human health . Medical research has proved that Ganoderma lucidum is not only effective for diseases such as hepatitis, arthritis, diabetes, high blood pressure, hyperlipidemia, and neurasthenia, but also has significant curative effects on three major human fatal diseases, coronary heart disease, cancer, and cerebral hemorrhage; it has anti-tumor, Improving the body's immunity, anti-virus, scavenging free radicals, anti-aging, anti-thrombosis, anti-coagulation and other functions; Hypoxic ability and cholesterol-lowering effect, have significant curative effect on coronary heart disease, high blood pressure, hyperlipidemia, hepatitis, myasthenia and other diseases; the nucleoside components in Songshan Ganoderma lucidum adenosine, uridine, adenine, uracil and other active ingredients are the material basis for the anti-mutation function of Ganoderma lucidum; the fruiting body of Ganoderma lucidum contains 18 kinds of amino acids, of which the content of essential amino acids for human body is 40% higher than that of other edible fungi, especially the lower content of methionine in edible fungi , the average content of pine, fir and ganoderma is much higher than other edible fungi. Studies have shown that the fruiting body of Ganoderma lucidum contains a variety of trace elements such as Mn, Cr, Cu, Fe, Ca, Mg, Zn, Se, Ge, etc. Ganoderma lucidum also contains a large amount of vitamins, of which VitC, VitE and carotene are relatively high. High, with significant antioxidant and anti-aging activities.

At present, my country's Ganoderma lucidum industry is mainly based on planting, and it is a major producer and exporter of Ganoderma lucidum in the world. However, farmer cultivation is the mainstay, and there are few large-scale and standardized plantings. The research and development of new planting and cultivation technology is not strong, and most of the planting methods use logs as raw materials, which consumes a lot of forest resources. At the same time, the quality of Ganoderma lucidum is difficult to control due to the farming method with farmers as the main body, resulting in poor quality of some Ganoderma lucidum, excessive pesticide residues and heavy metals, and insufficient supply of high-quality Ganoderma lucidum, making it difficult to provide high-quality raw materials for processed products.

Contents of the invention

In order to solve the above-mentioned technical problems, the present invention provides a method for cultivating pine fir ganoderma cultivars, a culture medium and a preparation method for the culture medium, so as to provide guidance for standardized artificial cultivation of pine fir ganoderma.

The present invention is achieved in this way. On the one hand, it provides a culture medium of pine, fir and ganoderma lucidum, which comprises 25-30% of sawdust, 35-40% of cottonseed husk, 15-20% of bran, and 8-12% of corncob by mass percentage. %, cornmeal 2-5%, gypsum 1-3%, superphosphate 0.5-1.5%, lime 0.5-1.5%, the total water content of each of the above ingredients is 60-70%, and 0.5-0.7g sub sodium selenate.

Further, by mass ratio, it includes 28% sawdust, 37% cottonseed husk, 18% bran, 10% corncob, 3% cornmeal, 2% gypsum, 1% superphosphate, and 1% lime. The water content is 65%, and 0.6g sodium selenite is added in every kilogram of culture medium.

The present invention also provides a method for preparing a pine fir ganoderma cultivar culture medium, comprising the steps of:

1) Use logs or sawdust of one or more kinds of pine trees in sand pine, black pine, and larch, crush them, heat them to 95-105°C, cook them for 8-10 hours, and let the pine wood particles fully exert their oil and antibacterial substances. After drying, save it into a large pile of base materials for later use;

2) Weigh each component of the culture medium according to any of the above-mentioned formulas, and mix them;

3) Sterilize the culture medium in step 2) in an autoclave with a pressure of 0.15 MPa for 1.5-2 hours and lower it to below 30°C;

4) Put the 5kg bulk base material prepared in step 1) and the sterilized 1.2kg medium in step 3) into a plastic bag, and mix them evenly to obtain the culture medium of Pinus fir ganoderma lucidum.

The present invention also provides a method for cultivating pine fir ganoderma cultivars, comprising the steps of:

a) according to the above-mentioned method, prepare the pine, fir and ganoderma cultivar culture medium;

b) In a sterile box, inoculate the parent species of Ganoderma lucidum on the medium, put the inoculated plastic bag in a sterilized, ventilated and dry cultivation room, and cultivate it for 40-50 days at a temperature of 23-27°C until the hyphae overgrown with plastic bags;

c) After fumigation and disinfection of the inoculated section wood with an aerosol disinfection box for 25-35min, tie it into bundles, open the plastic bag in step b), and sprinkle the culture medium covered with mycelium in the plastic bag into the section wood near the alcohol flame After the surface and gaps of the bundle, put the wooden bundle into a plastic bag and tie it immediately;

d) Put the plastic bag with the bundled bundles of seeds in the cultivation room, the temperature is 26-30°C, the humidity is 40-65%, ventilate for 6-8 hours every day, and loosen the tie rope after 15-20 days of cultivation Continue to cultivate until 45-55 days, the mycelium is full of bags, and then continue to cultivate for 15-20 days, until the mycelia are physiologically mature and form a bacterial bag, they can go to the ground.

Further, the bacterium bag cultivated in step d) is carried out under the forest imitating wild supporting cultivation: the bacterium bag with mycelia physiological maturity is placed in the forest stand in the last ten days of a month in May, and the width 80-100cm is placed between rows along the slope, 30-50m long double-layer bacterial bag, the distance between the bacterial bag is 5-10cm, the mixture of overlying soil and biomass is 1cm, keep the furrow surface moist, and set up a plastic film shed on the furrow; after the young mushrooms are cultivated to break the soil and emerge, the water management follows dry Wet alternately, that is, close the two ends of the plastic film shed at night to moisturize, and open it during the day; place a micro-spray in the plastic film shed, spray for 10-20 minutes in the morning, noon and evening, and reduce the water spray when the edge of the ganoderma fruiting body cap turns reddish brown ;

When the white growth circle on the edge of the cap begins to disappear, the color also begins to change from light to dark, the cap no longer increases, and when the spores are continuously distributed, harvest the fruiting body of Ganoderma lucidum.

Further, carry out the imitation wild cultivation of the undergrowth stump with the fungus package cultivated in step d): in late May, cut the pine wood with a diameter of 20-30cm into a 50cm long wood section, and drill a piece of wood with an electric drill with a diameter of 110cm. A hole with a length of 300mm is reserved. Transport the fungus bag and pine section to the cultivation forest, dig a pit on the ground with a diameter of 130cm and a depth of 40cm, fill each hole with about 200 fungus bags and peel off the outer skin of the fungus bag, and then stand the pine section on the fungus bag In the pit, the fungus bag is also placed in the hole of the wood section, and then covered with soil 20cm, the pine section is exposed 30cm from the soil surface, the fungus bag is completely buried, the distance between each hole of Ganoderma lucidum is ≥1m, and the growth temperature in the later stage is controlled at 26-28°C. The relative humidity of the air is about 80-90%, and the ventilation is 8-10 hours a day;

When the white growth circle on the edge of the cap begins to disappear, the color also begins to change from light to dark, the cap no longer increases, and when the spores are continuously distributed, harvest the fruiting body of Ganoderma lucidum.

Compared with the prior art, the present invention has the advantages of:

Provided are a culture medium and a culture method for cultivating the cultivars of the pine, fir and ganoderma lucidum, which can make the fruiting bodies of the pine, fir and ganoderma lucidum compact in texture, good in quality, and orderly in production, thereby increasing the yield and harvest of the pine, fir and ganoderma lucidum.

Detailed ways

In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

Four kinds of medium ingredients were designed, compared and screened out the ingredients suitable for the best cultivar culture medium of Pinus fir Ganoderma lucidum. A: 88% cottonseed husk, 10% bran, 1% sugar, 1% gypsum, 65% water content; B: 77% sawdust, 18% bran, 3% cornmeal, 1% sugar, 1% gypsum, water content 65%; C: 40% corncob, 45% sawdust, 10% bran, 4% cornmeal, 1% gypsum, 65% water content; D: 28% sawdust, 37% cottonseed husk, 18% bran, corncob 10%, cornmeal 3%, gypsum 2%, superphosphate 1%, lime 1%, water content 65%.

The effects of 4 different cultivar culture media on the sporocarp of Pinus fir Ganoderma lucidum are shown in Table 1. As can be seen from Table 1, among the 4 cultivar media tested, the Pinus fir Ganoderma strains were cultured in the A ratio cultivars. Based on the highest yield, the total yield reached 5050g, but the texture of the fruiting body was loose; the yield of the pine, fir and Ganoderma lucidum strain ranked second on the D ratio medium, with a total yield of 4400g, but the fruiting body was compact and of good quality. Therefore, the comprehensive evaluation of the Songshan Ganoderma lucidum strain on the D ratio cultivar culture medium is the highest, and the biological efficiency ranks second, reaching 10.5%. The output of Ganoderma lucidum fruiting body, adding a certain amount of sawdust in the ingredients helps to improve the quality of the fruiting body.

Table 1

Embodiment 1,

The cultivation method of the pine fir ganoderma cultivar comprises the steps:

1) Use logs or sawdust of one or more kinds of pine trees among sand pine, black pine, and larch, crush them, heat them to 100°C and cook them for 9 hours, dry them in the air to let the pine wood particles fully exert their oil and antibacterial substances, and store them after drying completely Make a large pile of base materials for use;

2) Weigh each component of the culture medium according to the following formula and mix evenly: by mass percentage, it includes 28% sawdust, 37% cottonseed husk, 18% bran, 10% corn cob, 3% cornmeal, 2% gypsum, Superphosphate 1%, lime 1%, the total water content of each of the above components is 65%, and 0.6g of sodium selenite is added per kilogram of medium.

3) Sterilize the culture medium in step 2) in an autoclave with a pressure of 0.15 MPa and a time of 1.7 hours, and lower it to below 30°C;

4) Put the 5kg bulk base material prepared in step 1) and the sterilized 1.2kg medium in step 3) into a plastic bag, and mix them evenly to obtain the culture medium of Pinus fir ganoderma lucidum.

5) In a sterile box, inoculate the parent species of Ganoderma lucidum on the medium, put the inoculated plastic bag in a sterilized, ventilated and dry cultivation room, and cultivate it for 45 days at a temperature of 25°C until the hyphae are covered with plastic bags ;

6) Fumigate and sterilize the inoculated section wood with an aerosol disinfection box for 30 minutes, tie it into bundles, open the plastic bag in step 5), and scatter the culture medium covered with mycelium in the plastic bag into the section wood bundle near the alcohol flame. After the surface layer and the gap, put the wooden bundle into a plastic bag and tie it immediately;

7) Place the plastic bag of the bundled wood section that has been planted in the cultivation room at a temperature of 28°C and a humidity of 42%, and ventilate for 7 hours every day. After 17 days of cultivation, loosen the tie rope and continue to cultivate until 50 days. bag, and continue to cultivate for another 17 days until the hyphae are physiologically mature and form a fungus bag, and then they can go to the ground.

The bacterium bag cultivated in step 7) is carried out under the forest to imitate wild supporting cultivation: the bacterium bag with mycelia physiological maturity is placed in the forest stand in the second ten days of a month in May, and the double layer of 90 cm wide and 40 m long is placed between rows along the slope Bacteria bag, the distance between the fungus bag is 7cm, the mixture of overlying soil and biomass is 1cm, keep the furrow surface moist, and set up a plastic film shed on the furrow; after the young mushrooms break out of the soil, water management follows the alternation of dryness and wetness, that is, close the plastic film shed at night Moisturize both ends of the Ganoderma lucidum, open it during the day; place a micro-spray in the plastic film shed, spray for 15 minutes in the morning, noon and evening, and reduce the water spray when the edge of the cap of the fruiting body of Ganoderma lucidum turns reddish brown;

When the white growth circle on the edge of the cap begins to disappear, the color also begins to change from light to dark, the cap no longer increases, and when the spores are continuously distributed, harvest the fruiting body of Ganoderma lucidum.

Embodiment 2,

The cultivation method of the pine fir ganoderma cultivar comprises the steps:

1) Use one or more kinds of pine logs or sawdust from sand pine, black pine, and larch, crush them, heat them to 95°C and cook for 8 hours, and let the pine wood particles fully exert their oil and antibacterial substances. Make a large pile of base materials for use;

2) Weigh each component of the culture medium according to the following formula, and mix evenly: by mass percentage, it includes sawdust 25%, cottonseed husk 35%, bran 15%, corncob 8%, cornmeal 2%, gypsum 1%, Superphosphate 0.5%, lime 0.5%, the total water content of each of the above components is 60%, and 0.5g sodium selenite is added per kilogram of medium.

3) Sterilize the culture medium in step 2) in an autoclave with a pressure of 0.15 MPa and a time of 1.5 hours, and lower it to below 30°C;

4) Put the 5kg bulk base material prepared in step 1) and the sterilized 1.2kg medium in step 3) into a plastic bag, and mix them evenly to obtain the culture medium of Pinus fir ganoderma lucidum.

5) In a sterile box, inoculate the parent ganoderma species on the culture medium, put the inoculated plastic bag in a sterilized, ventilated and dry cultivation room, and cultivate it for 40 days at a temperature of 23°C until the hyphae are covered with plastic bags ;

6) Fumigate and sterilize the inoculated section wood with an aerosol disinfection box for 25 minutes, tie it into bundles, open the plastic bag in step 5), and sprinkle the culture medium covered with mycelium in the plastic bag into the section wood bundle near the alcohol flame. After the surface layer and the gap, put the wooden bundle into a plastic bag and tie it immediately;

7) Place the plastic bag of the bundled wood section that has been planted in the cultivation room at a temperature of 26°C and a humidity of 40%, and ventilate for 6 hours a day. After 15 days of cultivation, loosen the tie rope and continue to cultivate until 45 days. bag, and continue to cultivate for another 15 days until the hyphae are physiologically mature and form a fungus bag, and then they can go to the ground.

The fungus package cultivated in step 7) is carried out under the tree stump imitating wild cultivation: in late May, cut the pine wood with a diameter of 20cm into a 50cm long wood section, and drill a hole with a length of 300mm on the wood section with an electric drill with a diameter of 110cm for future use , transport the fungus bag and pine wood section to the cultivation forest, dig a pit with a diameter of 130 cm and a depth of 40 cm on the ground, fill each pit with about 200 fungus bags and peel off the outer skin of the fungus bag, and then stand the pine wood section in the fungus bag pit. Then put the fungus bag into the hole, then cover with soil 20cm, the pine section is exposed to the soil surface 30cm, the fungus bag is completely buried, the distance between each hole of Ganoderma lucidum is ≥1m, the later growth temperature is controlled at 26°C, and the relative air humidity is kept at about 80%. , ventilated for 8 hours a day;

When the white growth circle on the edge of the cap begins to disappear, the color also begins to change from light to dark, the cap no longer increases, and when the spores are continuously distributed, harvest the fruiting body of Ganoderma lucidum.

Embodiment 3,

The cultivation method of the pine fir ganoderma cultivar comprises the steps:

1) Use one or more types of pine logs or sawdust from sand pine, black pine, and larch, crush them, heat them to 105°C, cook for 10 hours, and let the pine wood particles fully exert their oil and antibacterial substances. Make a large pile of base materials for use;

2) Weigh each component of the culture medium according to the following formula, and mix it evenly: by mass percentage, it includes 30% sawdust, 40% cottonseed husk, 20% bran, 12% corn cob, 5% cornmeal, 3% gypsum, Superphosphate 1.5%, lime 1.5%, the total water content of each of the above components is 70%, and 0.7g sodium selenite is added per kilogram of medium.

3) Sterilize the culture medium in step 2) in an autoclave at a pressure of 0.15 MPa for 2 hours and lower it to below 30°C;

4) Put the 5kg bulk base material prepared in step 1) and the sterilized 1.2kg medium in step 3) into a plastic bag, and mix them evenly to obtain the culture medium of Pinus fir ganoderma lucidum.

5) In a sterile box, inoculate the parent species of Ganoderma lucidum on the medium, put the inoculated plastic bag in a sterilized, ventilated and dry cultivation room, and cultivate it for 50 days at a temperature of 27°C until the hyphae are covered with plastic bags ;

6) Fumigate and sterilize the inoculated section wood with an aerosol disinfection box for 35 minutes, tie it into bundles, open the plastic bag in step 5), and scatter the culture medium covered with mycelium in the plastic bag into the section wood bundle near the alcohol flame. After the surface layer and the gap, put the wooden bundle into a plastic bag and tie it immediately;

7) Place the plastic bag of the bundled wood section that has been planted in the cultivation room at a temperature of 30°C and a humidity of 65%, and ventilate for 8 hours a day. After 20 days of cultivation, loosen the tie rope and continue to cultivate until 55 days. bag, and continue to cultivate for another 20 days until the hyphae are physiologically mature and form a fungus bag, and then they can go to the ground.

The bacterium bag cultivated in step 7) is carried out under the forest to imitate wild supporting cultivation: the bacterium bag with mycelia physiological maturity is placed in the stand in late May, and placed between rows along the slope as a double-layer with a width of 100 cm and a length of 50 m Bacteria packs, the distance between the packs is 10cm, the mixture of overlying soil and biomass is 1cm, keep the furrow surface moist, and set up a plastic film shed on the furrow; after the young mushrooms break out of the soil, the water management follows the alternation of dryness and wetness, that is, close the plastic film shed at night Moisturize both ends of the Ganoderma lucidum, open it during the day; place a micro-spray in the plastic film shed, spray for 20 minutes in the morning, noon and evening, and reduce the water spray when the edge of the cap of the fruiting body of Ganoderma lucidum turns reddish brown;

When the white growth circle on the edge of the cap begins to disappear, the color also begins to change from light to dark, the cap no longer increases, and when the spores are continuously distributed, harvest the fruiting body of Ganoderma lucidum.

The Ganoderma lucidum obtained in Examples 1, 2, and 3 is detected, and it can be found that sodium selenite can increase the yield of Ganoderma lucidum fruiting bodies, increase the selenium content in the Ganoderma lucidum, and the fruiting is neat, and improve the protein, polysaccharide, and amino acid content in the Ganoderma lucidum.

Claims (6)

1. a kind of Ganoderma tsugae Cultivar culture medium, which is characterized in that by mass percentage, including sawdust 25-30%, cotton seed hull 35-40%, wheat bran 15-20%, corncob 8-12%, maize flour 2-5%, gypsum 1-3%, calcium superphosphate 0.5-1.5%, lime 0.5-1.5%, above-mentioned each ingredient total moisture content 60-70%, and add 0.5-0.7g sodium selenite in every kg broth.

2. Ganoderma tsugae Cultivar culture medium as described in claim 1, which is characterized in that in mass ratio, including sawdust 28%, Cotton seed hull 37%, wheat bran 18%, corncob 10%, maize flour 3%, gypsum 2%, calcium superphosphate 1%, lime 1%, it is above-mentioned each Ingredient total moisture content 65%, and add 0.6g sodium selenite in every kg broth.

3. a kind of preparation method of Ganoderma tsugae Cultivar culture medium, which comprises the steps of:

1) using the log or sawdust of one or more pine trees in abies holophylla, black pine, larch, 95-105 DEG C of steaming is heated up to after crushing It boils 8-10 hours, sunning allows particles of pine to give full play to grease and antibacterial substance, and it is spare that raft base-material is collected together into after drying completely；

2) each ingredient that culture medium is weighed according to formula as described in claim 1 or 2 is any, mixes；

3) culture medium in step 2) is sterilized in high-pressure steam sterilizing pan, pressure 0.15MPa, time 1.5-2h are down to 30 DEG C or less；

4) culture medium of the 1.2kg to sterilize in the 5kg raft base-material prepared in step 1) and step 3) is fitted into polybag, is mixed It closes uniformly, obtains Ganoderma tsugae Cultivar culture medium.

4. a kind of cultural method of Ganoderma tsugae cultivar, which comprises the steps of:

A) Ganoderma tsugae Cultivar culture medium is prepared according to the method for claim 3；

B) in sterile board, toward culture medium on be inoculated with lucid ganoderma stock culture, by inoculated polybag be placed on by sterilizing, aeration-drying Culturing room in, 23-27 DEG C of temperature, culture 40-50 days, until mycelia covers with polybag；

C) bundle will be bundled into after inoculation Duan Muyong aerosol disinfection box fumigation 25-35min, the polybag in opening steps b), After the culture medium for covering with mycelia in polybag is nearby sprinkled into the surface layer and gap of section wood bundle by Alcohol Flame, section wood bundle is put into modeling Tying immediately in material bag；

D) polybag equipped with the section wood bundle for connecting kind is placed in culturing room, temperature is 26-30 DEG C, humidity 40-65%, daily Ventilation 6-8 hours, culture loosen tying rope and continue culture to 45-55 days after 15-20 days, mycelia purseful is cultivated for 15- 20 days, until mycelia physiological maturity, forms bacterium bag, can descend ground.

5. the cultural method of Ganoderma tsugae cultivar as claimed in claim 4, which is characterized in that will be cultured in step d) Bacterium bag carries out hayashishita and imitates wild corresponding cultivation: the bacterium bag of mycelia physiological maturity is placed in standing forest by late May, is in the ranks put along slope It puts as wide 80-100cm, the double-deck bacterium bag of long 30-50m, bacterium bag spacing is 5-10cm, the mixture 1cm of upper earthing and biomass, It keeps furrow face wet, plastic film canopy is set on furrow；After culture breaks ground to young sesame and shows up, water management follows alternation of wetting and drying, i.e. night Between close plastic film canopy both ends moisturizing, daytime open；Micro- spray is disposed in plastic film canopy, respectively sprays 10-20min in the morning, afternoon and evening, When the cap edge of ganoderma lucidum fruitbody becomes bronzing, water spray is reduced；

When white growth circle in cap edge starts to disappear, color also starts from light to dark, and cap no longer increases, and spore constantly distributes When, harvest ganoderma lucidum fruitbody.

6. the cultural method of Ganoderma tsugae cultivar as claimed in claim 4, which is characterized in that will be cultured in step d) Bacterium bag carries out hayashishita stump pseudo-wild cultivating: the pine of diameter 20-30cm is cut into the juggle of 50cm long by late May, uses diameter The hole that the electric drill of 110cm bores a length 300mm on juggle is spare, and bacterium bag and pine section are transported in cultivation woods, dug on the ground Diameter 130cm, the hole of deep 40cm, every hole fill 200 or so bacterium bags and remove bacterium bag crust, and pine section is then stood on bacterium bag In hole, bacterium bag is also placed in the hole of juggle, then earthing 20cm, pine section exposes soil face 30cm, and bacterium bag is all buried sternly, each cave Spacing >=1m of ganoderma lucidum, late growth temperature are controlled at 26-28 DEG C, are kept relative air humidity in 80-90% or so, are led to daily Wind 8-10 hours；

When white growth circle in cap edge starts to disappear, color also starts from light to dark, and cap no longer increases, and spore constantly distributes When, harvest ganoderma lucidum fruitbody.