CN108939158A - Small intestine acellular matrix film combination metal mesh breast wall repairing material - Google Patents

Abstract

The invention provides a small intestinal acellular matrix membrane combined with metal mesh chest wall repair material, which belongs to the field of medical biomaterials. The material includes: processing the small intestine cut off from an animal to produce a small intestinal acellular matrix membrane, and then combining the small intestinal acellular matrix membrane with a metal mesh to prepare a chest wall patch material. The preparation method of the material of the invention is simple, and the obtained chest wall patch material is used for repairing the chest wall of patients with chest wall defects, and has broad development prospects.

Description

Small intestinal acellular matrix membrane combined with metal mesh chest wall repair material

technical field

The invention belongs to the field of medical biomaterials, and in particular relates to a small intestine acellular matrix membrane combined with metal mesh chest wall repair material.

Background technique

In clinical practice, many patients with severe trauma have chest wall defects. When the defect area is large, it is easy to cause mediastinal oscillation, and the life of the patient is threatened. The conventional operation is only a partial repair, or a part of the tissue or organ from other parts of the body is used for repair. At the same time, it caused some damage. With the progress of related research on small intestinal acellular matrix membrane (ECM) biological patch, our scientific research team has developed a small intestinal acellular matrix membrane combined with metal mesh chest wall repair material, which has a wide range of clinical applications and can be applied to the pleura, Repair of chest wall, skull, etc. The patch material has broad development prospects and will definitely promote the development of artificial biomaterials.

Contents of the invention

The object of the present invention is to provide a small intestine acellular matrix membrane combined with metal mesh chest wall repair material, the preparation material has a simple structure, and the obtained chest wall patch material plays a huge role in promoting the preparation of artificial organs.

The present invention firstly provides a small intestinal acellular matrix membrane combined with metal mesh chest wall repair material:

Step 1: using the small intestine cut off from the animal to prepare the acellular matrix membrane;

Step 2: using titanium-nickel alloy materials to prepare metal meshes of different sizes, the metal meshes are spider-like titanium-nickel alloy metal meshes that protrude slightly toward the plane side;

Step 3: fixing the small intestinal acellular matrix membrane obtained in step 1 on the metal mesh obtained in step 2 to obtain a chest wall patch material;

Step 4: The chest wall patch material obtained in Step 3 is irradiated and sterilized with a radiation dose of 8000 rads by linear acceleration, and then sealed and stored.

Preferably, the animals in step 1 are pigs, cattle, sheep, rats, and horses.

Preferably, the metal in step 1 is titanium-nickel alloy.

Preferably, the acellular matrix membrane in the step 1 is a small intestinal acellular matrix membrane obtained by processing the small intestine cut from a living animal.

Preferably, the advantages of the metal mesh in the second step are specifically: the metal mesh of different sizes is made of titanium-nickel alloy material, the metal mesh has certain toughness and elasticity, and cannot change with the pressure difference inside and outside the thoracic cavity after being implanted in the chest wall And generate activity.

Preferably, the method for combining the acellular matrix membrane and metal mesh in step three is as follows: fixing the small intestine acellular matrix membrane obtained in step one on the metal mesh obtained in step two to obtain the chest wall patch material.

Preferably, the sterilization in the fourth step is to preserve the sealed chest wall patch material after being irradiated and sterilized with a radiation dose of 8000 rads through linear acceleration.

The invention provides a chest wall patch material in which the acellular matrix membrane of the small intestine is combined with a metal mesh obtained by the above preparation method.

Beneficial effects of the present invention

The present invention firstly provides a small intestinal acellular matrix membrane combined with metal mesh chest wall repair material, wherein the metal material is a titanium-nickel alloy material, which causes few adverse reactions and has been widely used in human body repair or as a fracture fixation device. human body. The metal net is a spider web-like structure with a slightly protruding central point. This structure has better stability and is not easily deformed. It can resist greater tension with less material and reduce the impact of metal substances on the human body. The combination of the acellular matrix membrane and the metal mesh is that the metal mesh is fixed on a specific support device, and the small intestinal acellular matrix membrane is flattened along the diameter of the circular metal mesh and wound on both sides of the metal mesh, so that it can be combined with The metal mesh is tightly bound together.

Description of drawings

Fig. 1 is a photographic view of a single-layer natural tubular porcine small intestinal acellular matrix membrane prepared by the present invention.

Fig. 2 is a photograph of the single-layer natural tubular porcine small intestinal acellular matrix membrane prepared by the present invention before being combined with a metal mesh and dried.

Fig. 3 is a photograph of a single-layer natural tubular porcine small intestinal acellular matrix membrane prepared by the present invention combined with a metal mesh and dried.

Detailed ways

The present invention firstly provides a small intestine acellular matrix membrane combined with metal mesh chest wall repair material, the material includes two parts, small intestine acellular matrix membrane and metal mesh, its preparation method is as follows:

Step 1: Take a local white pig with a weight of 50-100Kg, use the preparation method of "Natural Tubular Small Intestine Acellular Matrix Membrane and Its Preparation Method" invented by our research group to prepare the natural tubular acellular matrix membrane of the small intestine, and then Cut it longitudinally to obtain a sheet of acellular matrix membrane.

Step 2: Use titanium-nickel alloy materials to make metal meshes of different sizes. The metal meshes have certain toughness and elasticity. After implanting in the chest wall, they cannot move with changes in the pressure difference between the inside and outside of the thoracic cavity. The diameters of titanium-nickel alloy wires used in this experiment are: 1.0mm and 0.8mm. First take two titanium-nickel alloy wires with a diameter of 1.0mm and a length of 10cm and cross them and weld the intersection points together. Weld another group in the same way. Use the welding point as the center of the two groups of cross-welded wires to make a circle with an average of 8 Weld the 2 welding points together after equal parts, flatten the welding points so that the thickness does not exceed 2mm, and slightly protrude the center point to one side of the plane. Take a titanium-nickel alloy wire with a diameter of 0.8mm and a length of 50cm, and make it into a spiral with one end as the center, and keep all the wires on a plane, and the distance between two adjacent wires is 5.0mm. Superimpose the metal wires of the above two specifications, and weld the overlapping wires to obtain a cobweb-shaped metal mesh with the center slightly protruding to the side of the plane.

Step 3: Fix the small intestinal acellular matrix membrane obtained in step 1 on the metal mesh obtained in step 2 to obtain a chest wall patch. First, fix the metal mesh obtained in step 2 on a specific support, spread the small intestinal acellular matrix membrane obtained in step 1 on the metal mesh obtained in step 2, and wrap it repeatedly so that the acellular matrix membrane overlaps 2-4 layers, keeping Some tension to avoid wrinkling and to lock the edge to the edge of the wire mesh.

Step 4: The chest wall patch material obtained in Step 3 is irradiated and sterilized with a radiation dose of 8000 rads by linear acceleration, and then sealed and stored.

According to the present invention, the small intestine cut from an animal is processed to obtain the small intestinal acellular matrix membrane, and the animal is not particularly limited, preferably pig, cow, sheep, mouse or horse.

According to the present invention, the metal wire obtained by processing the titanium-nickel alloy is processed to obtain a metal mesh, and the metal mesh is made of metal wire, preferably a titanium-nickel alloy material.

According to the present invention, the preparation of the small intestinal acellular matrix membrane is to adopt the preparation method in "Natural Tubular Small Intestine Acellular Matrix Membrane and Its Preparation Method" invented by our research group, and then the tubular acellular matrix membrane is taken out and then the tubular acellular matrix membrane is removed. The cell matrix membrane was dissected longitudinally to obtain a sheet of acellular matrix membrane of the small intestine.

According to the present invention, the metal mesh adopts titanium-nickel alloy wire as the main material.

According to the present invention, the small intestine acellular matrix membrane combined with metal mesh chest wall repair material obtained above is dried, sealed, sterilized and stored for future use. Preferably, the sterilization is to sterilize the chest wall patch material prepared by combining the sealed intestinal acellular matrix membrane with the metal mesh and irradiating it with a radiation dose of 8000 rads in a linear manner.

The following are used to illustrate the present invention, but are not intended to limit the scope of the present invention. If not specified, the technical means used in the present invention are conventional means well known to those skilled in the art, and the raw materials used are commercially available items.

1. Preparation of flake ECM

Take 1 local white pig with a weight of 50-100Kg, use the preparation method of "Natural Tubular Small Intestine Acellular Matrix Membrane and Its Preparation Method" invented by our research group to prepare the natural tubular acellular matrix membrane of the small intestine, and then longitudinally Cut open to obtain a sheet of acellular matrix membrane.

2. Preparation of titanium-nickel alloy metal mesh

A titanium-nickel alloy wire is taken to produce a cobweb-shaped metal mesh whose center slightly protrudes toward the plane side.

Fig. 1 is the photograph diagram of single-layer porcine small intestine acellular matrix membrane of the present invention; Fig. 2 is the photograph diagram of the present invention single-layer lamellar porcine small intestine decellularized matrix membrane combined with metal mesh before drying;

Fig. 3 is a photograph of a single-layer sheet porcine small intestinal acellular matrix membrane combined with a metal mesh and dried; Fig. 2 to Fig. 3 illustrate that the acellular matrix membrane and the metal mesh are tightly combined, and the small intestinal acellular matrix membrane is successfully prepared Combined with metal mesh chest wall repair material.

Claims (6)

1. Small intestine acellular matrix membrane combined with metal mesh chest wall repair material, characterized in that the production of the material includes: Step 1: using the small intestine cut off from the animal to prepare the acellular matrix membrane; Step 2: Use titanium-nickel alloy materials to make metal meshes of different sizes. The metal meshes have certain toughness and elasticity. After implanting in the chest wall, they cannot move with changes in the pressure difference between the inside and outside of the thoracic cavity; Step 3: fixing the small intestinal acellular matrix membrane obtained in step 1 on the metal mesh obtained in step 2 to obtain a chest wall patch material; Step 4: The chest wall patch material obtained in Step 3 is irradiated and sterilized with a radiation dose of 8000 rads by linear acceleration, and then sealed and stored. 2. A kind of small intestinal acellular matrix membrane combined with metal mesh chest wall repair material according to claim 1, characterized in that the animals in the first step are pigs, cattle, sheep, rats, and horses, and the metal material is titanium nickel alloy. 3. A kind of small intestine decellularized matrix membrane combined with metal mesh chest wall repair material according to claim 1, characterized in that, the decellularized matrix membrane in the described step 1 is the acellular matrix membrane produced from the small intestine of living animals . 4. A small intestinal acellular matrix membrane combined with metal mesh chest wall repair material according to claim 1, characterized in that the metal material in the second step is a titanium-nickel alloy material, which has few adverse reactions and has been Widely used in the repair of the human body or as a fixation device for fractures in the human body, the metal mesh is a spider web-like structure with the center point slightly protruding to the side of the plane. This structure has better stability, is not easy to deform, and can resist with less material Greater tension, reducing the impact of metal substances on the human body. 5. A small intestinal acellular matrix membrane combined with metal mesh chest wall repair material according to claim 1, characterized in that the combination of the small intestine acellular matrix membrane and the metal mesh in step 3 is specifically: fixing the metal mesh on On a specific stent device, the acellular matrix membrane of the small intestine is flattened along the diameter of the circular metal mesh and wound on both sides of the metal mesh, so that it can be tightly combined with the metal mesh. 6. A kind of small intestine acellular matrix membrane combined metal mesh chest wall repair material according to claim 1, is characterized in that, the sterilization of described step 4 is that the chest wall patch material that seals is accelerated linearly with 8000 pulls German radiation dose irradiation sterilization.