CN108815280A - A kind of Chinese medicine composition and its preparation method and application deforming pseudomonad disease for treating grouper - Google Patents

Abstract

The invention relates to a traditional Chinese medicine composition for treating grouper pseudomonas metamorphosis, a preparation method and application thereof, and belongs to the technical field of aquaculture. The traditional Chinese medicine composition for treating grouper pseudomonas metamorphosis comprises Coptis Rhizoma, Radix Astragali and Cortex Phellodendri; the mass ratio of Coptis Rhizoma, Radix Astragalus and Cortex Phellodendri is 0.9-1.3:0.9-1.3:0.9-1.3. The prepared traditional Chinese medicine composition can greatly improve the efficacy of medicine, and the survival rate of diseased fish without medicine is increased by more than 60%. The composition provided by the invention has the advantages of scientific compatibility, low cost, short course of treatment, quick effect and high cure rate, and provides help for the prevention and treatment of fish diseases in aquaculture and production.

Description

A traditional Chinese medicine composition for treating grouper pseudomonadosis metamorphoses and its preparation Methods and Applications

technical field

The invention belongs to the technical field of aquaculture, and in particular relates to a traditional Chinese medicine composition for treating grouper pseudomonas metamorphosis, a preparation method and application thereof.

Background technique

Pseudomonas plecoglossicida has been proved to be the pathogen of white spot disease in marine fish viscera in eastern Fujian. It is a common disease of seawater fish cultured in net cages, causing large-scale death of large yellow croakers and causing serious losses to the aquaculture industry. It is a typical temperature-dependent disease that breaks out at a water temperature of 16-20°C. When the water temperature exceeds 20°C The disease subsides spontaneously. Pseudomonas mutans can not only cause visceral white spot disease of cultured marine fish, but also can cause visceral white spot disease of grouper (Epinephelus) and other marine fish under artificial infection, and the mortality rate of infection is as high as 100%.

Antibiotics are currently the main means of treating bacterial diseases in aquatic animals. With the widespread use of antibiotics in the treatment of bacterial diseases, bacterial resistance and drug residues have occurred. And grouper self resistance is reduced day by day, and disease problem is serious day by day, has brought big loss to grouper aquaculture. Pseudomonas metamorphosis of grouper has caused serious economic losses. Western medicine is mainly used to treat fish diseases in aquaculture production. The bacteria have developed drug resistance and the treatment effect is not good. It is of great significance to research and develop Chinese herbal medicines to prevent and control fish diseases. In addition to overcoming the aforementioned shortcomings, traditional Chinese medicine in my country can also enhance the immunity of aquaculture animals. Experiments have shown that Chinese medicine and Chinese medicine compound have better inhibitory effect on bacteria in vitro. Retrieval Coptidis Rhizoma, Radix Astragali, Phellodendron Phellodendron all have a small amount of patents, but all are unilateral medicines, are used in the formula such as strengthening the disease prevention and treatment of animals, and obtain certain curative effect.

However, there are many patents on Pseudomonas mutans in the prior art, but they all use Pseudomonas mutans nitrile compounds as the only energy source for growth and reproduction, which can degrade pollutants such as nitrile compounds in acrylonitrile wastewater, or Arginine deiminase (ADI) produced by fermentation of the microorganism Pseudomonas mutans. In the prior art, the patents related to Pseudomonas mutans are all the use of the bacteria itself. There is no patent on the prevention and treatment of Pseudomonas mutans, and there is no report on the treatment plan for grouper Pseudomonas mutans.

Contents of the invention

In view of this, the object of the present invention is to provide a kind of Chinese medicine composition for the treatment of grouper Pseudomonas mutans disease and its preparation method and application, described Chinese medicine composition is effective to the disease that Pseudomonas mutans causes Strong medicinal effect.

In order to achieve the above-mentioned purpose of the invention, the present invention provides the following technical solutions:

The invention provides a traditional Chinese medicine composition for treating grouper pseudomonas metamorphosis, which comprises coptis, astragalus and cortex cortex; the mass ratio of the coptis, astragalus and cortex cortex is 0.9-1.3:0.9-1.3:0.9-1.3.

Preferably, the mass ratio of Coptidis Rhizome, Radix Astragali and Cortex Phellodendri is 1:1:1.

The invention provides the preparation method of described Chinese medicine composition, comprises the following steps:

1) Pulverizing Coptis Rhizoma, Radix Astragali and Cortex Phellodendri to obtain Chinese medicine powder;

2) Superfinely pulverizing the traditional Chinese medicine powder to obtain superfine medicine powder;

3) Baking the ultrafine medicinal powder at 60-70° C. for 24-48 hours to obtain a traditional Chinese medicine composition.

Preferably, the particle size of the traditional Chinese medicine powder in step 1) is 140-160 μm;

The particle size of the superfine drug powder in the step 2) is 5-10 μm.

Preferably, the baking temperature in step 3) is 65° C., and the baking time is 36 hours.

The invention provides an application of the traditional Chinese medicine composition in preparing a medicine for treating grouper pseudomonas metamorphosis.

Preferably, the effective antibacterial concentration of the traditional Chinese medicine composition is 60-80 mg/L.

Preferably, the feeding amount of the pharmaceutical composition is 1.8-2.4% of the mass of grouper feed.

Preferably, the drug is administered for 30 to 45 days for the treatment of grouper Pseudomonas metamorphosis.

The invention provides a traditional Chinese medicine composition for treating grouper pseudomonas metamorphosis, which combines Coptidis Rhizoma, Radix Astragali and Phellodendron Phellodendron according to the mass ratio of 0.9-1.3:0.9-1.3:0.9-1.3, and the compound medicine has synergistic effect. The effect can greatly improve the efficacy of single prescription medicine and reduce the dosage of each medicine. At the same time, the prepared Chinese medicine composition can greatly improve the efficacy of treating grouper Pseudomonas metamorphosis, which is better than that of diseased fish without treatment. Survival rate increased by more than 60%.

Simultaneously, the composition provided by the invention utilizes the triple traditional Chinese medicine formula to treat grouper pseudomonas metamorphoses. Compared with single Chinese medicine, it has the advantages of shorter treatment time, better curative effect, and less residue than pure western medicine.

The invention provides a preparation method of the composition. Chinese medicine components Coptidis Rhizome, Radix Astragali and Phellodendron Phellodendri are sequentially pulverized, superfinely pulverized and baked, and the obtained Chinese medicine fine powder is mixed to prepare the composition. The preparation method is conducive to the full release of the active ingredients in the traditional Chinese medicine components and improves the efficacy of the medicine. At the same time, the method has simple preparation steps, is produced by mechanical processing, has good repeatability, and is conducive to realizing mass production.

The invention provides an application of the traditional Chinese medicine composition in preparing a medicine for treating grouper pseudomonas metamorphosis. The composition can effectively inhibit the growth of Pseudomonas mutans, so that the prepared medicine can effectively treat Pseudomonas mutans disease of grouper.

Detailed ways

The invention provides a traditional Chinese medicine composition for treating pseudomonas metamorphoses of grouper, comprising Coptidis Rhizoma, Radix Astragali and Phellodendron Phellodendri; the mass ratio of Coptidis Rhizoma, Astragalus Radix and Phellodendron Phellodendri is 0.9～1.3:0.9～1.3:0.9～1.3, preferably 1:1:1.

The present invention has no special limitation on the sources of the Coptis Rhizoma, Radix Astragali, and Phellodendron Phellodendri, and Coptis Rhizoma, Radix Astragali, and Phellodendron Phellodendri well-known to those skilled in the art can be used. In the embodiment of the present invention, the Coptidis Rhizoma, Radix Astragali and Cortex Phellodendron were purchased from Lianfutang Pharmacy in Xiamen.

The invention provides the preparation method of described Chinese medicine composition, comprises the following steps:

1) Pulverizing Coptis Rhizoma, Radix Astragali and Cortex Phellodendri to obtain Chinese medicine powder;

2) Superfinely pulverizing the traditional Chinese medicine powder to obtain superfine medicine powder;

3) Baking the ultrafine medicinal powder at 60-70° C. for 24-48 hours, and the obtained Chinese medicine fine powder is a Chinese medicine composition.

The present invention grinds coptis, astragalus and cortex cortex to obtain traditional Chinese medicine powder. The particle size of the pulverized traditional Chinese medicine powder is preferably 140-160 μm. More preferably, it is 150 μm. The pulverization includes pulverizing Coptis Rhizoma, Radix Astragali and Cortex Phellodendri separately or mixing Coptidis Rhizoma, Radix Astragali and Phellodendron Phellodendri according to the above ratio and then pulverizing. The present invention has no special limitation on the crushing method, and the crushing method known to those skilled in the art can be used. In the embodiment of the present invention, the pulverizing instrument is a pulverizer with a model number of FW177, purchased from Tianjin Test Instrument Co., Ltd.

After the traditional Chinese medicine powder is obtained, the present invention carries out ultrafine pulverization on the traditional Chinese medicine powder to obtain the ultrafine medicinal powder.

In the present invention, the traditional Chinese medicine powder is preferably roasted before the ultrafine pulverization. The baking temperature is preferably 60-70°C, more preferably 65°C. The baking time is preferably 24-48 hours, more preferably 36 hours. Baking reduces the water content of the ultrafine powder, making it meet the requirements of ultrafine pulverization.

In the present invention, the particle size of the ultrafine drug powder is preferably 5-10 μm, more preferably 6-9 μm, most preferably 8 μm. In the present invention, there is no special limitation on the ultrafine pulverization method, and the ultrafine pulverization method well known in the art can be used. In the embodiment of the present invention, the ultrafine pulverization instrument is a Sanqing vibrating micronizer with a model number of SQW-60, purchased from Shandong Sanqing Stainless Steel Equipment Co., Ltd. The ultrafine pulverization is conducive to the full release of the active ingredients of the traditional Chinese medicine.

After obtaining the ultrafine medicinal powder, the present invention bakes the ultrafine medicinal powder at 60-70° C. for 24-48 hours to obtain the traditional Chinese medicine composition. In the present invention, the baking temperature is preferably 65° C., and the baking time is preferably 36 hours. Baking reduces the moisture content of the superfine powder.

The invention provides an application of the traditional Chinese medicine composition in preparing a medicine for treating grouper pseudomonas metamorphosis.

In the present invention, the bacteriostasis experiment of the Chinese medicine composition on Pseudomonas metamorphans of the grouper comprises the following steps: the Chinese medicine composition is prepared as a solution, and the obtained Chinese medicine composition solution has a bacterial concentration of 10 to 0.1 mL. ⁸ CFU/mL of Pseudomonas mutans was dropped into the plate, spread evenly, and observed the antibacterial situation. As a result, it was found that the effective concentration of the Chinese medicine composition solution was 60-80 mg/L, which was extremely sensitive. The effective concentration of the traditional Chinese medicine composition solution is more preferably 65-75 mg/L, most preferably 70 mg/L.

In the present invention, when the traditional Chinese medicine composition is used to prepare the medicine, the mass percentage of the Chinese medicine composition in the medicine is 1.8%-2.4%.

In the present invention, the preparation method of the medicine preferably includes the following steps: making the feed into feed fine powder, mixing the traditional Chinese medicine composition prepared by the above scheme with the feed fine powder, and granulating to obtain the medicine. The present invention has no special limitation on the mixing method, and a mixing method well known in the art can be used. The present invention has no special limitation on the granulation method, and the granulation method well known in the art can be adopted.

In the present invention, the pharmaceutical composition is added to the basal feed of the grouper to make pharmaceutical pellets, and the mass of the traditional Chinese medicine composition preferably accounts for 1.8% to 2.4% of the total mass of the medicine, more preferably 2.0%. The feeding amount of the traditional Chinese medicine composition is preferably 2%-3% of the fish body mass, more preferably 2.5%.

In the present invention, the drug is preferably administered for 30 to 45 days for the treatment of grouper Pseudomonas metamorphosis.

A kind of traditional Chinese medicine composition for treating grouper Pseudomonas metamorphosis provided by the present invention and its preparation method and application are described in detail below in conjunction with the examples, but they cannot be interpreted as limiting the protection scope of the present invention.

Example 1

Purchase Coptis chinensis, Radix Astragali, Phellodendron Phellodendri from Lianfutang Pharmacy in Xiamen; pulverize traditional Chinese medicine with a traditional Chinese medicine pulverizer (FW177, Tianjin Test Instrument Co., Ltd.) , SQW-60, Shandong Sanqing Stainless Steel Equipment Co., Ltd.) for further pulverization until the particle size is 5-10 μm, and the superfine powder is collected for later use. The three kinds of ultrafine medicinal powders were baked at 60° C. for 48 hours to obtain fine powders of traditional Chinese medicines; the fine powders of traditional Chinese medicines obtained above were weighed, 0.9 kg of Coptidis Rhizoma fine powder, 1.3 kg of Astragalus membranaceus fine powder, and 0.9 kg of Phellodendron Phellodendri fine powder, The traditional Chinese medicine composition is obtained.

Example 2

Pulverize the traditional Chinese medicines of Phellodendron Phellodendri, Coptidis Rhizoma, and Radix Astragali to 160 μm to obtain three kinds of traditional Chinese medicine powders; respectively ultrafinely pulverize the three kinds of traditional Chinese medicine powders to a particle size of 10 μm to obtain three kinds of ultrafine medicinal powders; Bake at 70°C for 24 hours to obtain fine powder of traditional Chinese medicine; weigh the fine powder of traditional Chinese medicine obtained above, 1.2 kg of fine powder of coptis, 0.9 kg of fine powder of astragalus, and 1.0 kg of fine powder of Cortex Phellodendri, and mix to obtain a traditional Chinese medicine composition.

Example 3

Pulverize the Chinese medicines of Phellodendron Phellodendri, Coptidis Rhizoma, and Radix Astragali to 150 μm to obtain three kinds of Chinese medicine powders; respectively ultrafinely pulverize the three kinds of Chinese medicine powders to a particle size of 8 μm to obtain three kinds of ultrafine medicine powders; Bake at 65°C for 36 hours to obtain fine powder of traditional Chinese medicine; weigh the fine powder of traditional Chinese medicine obtained above, 1.0 kg of fine powder of coptis, 1.0 kg of fine powder of astragalus, and 1.0 kg of fine powder of cork phellodendron, and mix to obtain a traditional Chinese medicine composition.

Comparative example 1

Weigh 1.0 kg of Coptidis rhizoma fine powder prepared in Example 3 to obtain a single prescription.

Comparative example 2

Weigh 2.0 kg of Astragalus fine powder and 2.0 kg of Phellodendron Phellodendri fine powder prepared in Example 3 to obtain a compound dual Chinese medicine composition.

Comparative example 3

Purchase Forsythia from Jiangyin Tianjiang Pharmaceutical Co., Ltd., prepare Forsythia Chinese medicine fine powder according to the method of Example 3, weigh 1.5kg of Forsythia Chinese medicine fine powder, and mix with Coptidis fine powder 1.5kg and Astragalus fine powder prepared in Example 3. Powder 1.5kg mixes, obtains compound prescription triple Chinese medicine composition.

Comparative example 4

Using the Oxford cup method, refer to the drug concentration of enrofloxacin drug-sensitive discs in the KB disc agar diffusion method recommended by WHO (Gui Bingdong et al., 2001), and add the drug at the above concentration into each well. Enrofloxacin (Xinghua Hengwei Biotechnology Co., Ltd.). Spread 1×10 ⁸ CFU/mL Pseudomonas mutans 200 μL bacterial suspension evenly on the LB medium plate, and judge the result according to the latest standard of CLSIM100. The diameter of the inhibition zone was measured to determine the sensitivity of Pseudomonas mutans and Pseudomonas mutans.

Embodiment 4～6

The composition prepared in Example 1 was mixed with water, and heated over water to prepare solutions with mass concentrations equivalent to crude drugs of 60 mg/ml, 70 mg/ml and 80 mg/ml. The compositions prepared in Examples 2 and 3 were also prepared into 60 mg/ml, 70 mg/ml and 80 mg/ml drug solutions according to the above method.

Use the Oxford cup method to study the effect of drugs on bacteria. Pour 10-12mL of sterilized LB solid medium into the plate. After solidification, use tweezers to gently place 4 Oxford cups in each petri dish at equal distances. Immerse in the medium, take 12-15mL of LB medium (the temperature should not be hot), mix it evenly and pour it on the plate, after it solidifies, remove the Oxford cup, and dilute Pseudomonas mutans to 1.0x10 ⁸ ~ 3.0x10 ⁸ CFU/mL, draw 0.1mL of bacterial suspension with a pipette gun, drop it into the plate, spread it evenly with a spreader, add 200 μL of sterile water into one well with a pipette gun as a control, and in the other 3 Add 200 μL of the above-mentioned three different concentrations of medicinal solutions to each well with a pipette gun, and set up three replicate groups for each test group and control group. Placed at 26-30°C, cultured at constant temperature for 18-36 hours, observed the inhibition zone, and measured the size of the inhibition zone with SupcreG1 colony counting/screening/inhibition zone measurement instrument (Hangzhou Xunshu Technology Co., Ltd.). The experiment was repeated 3 times. Calculate the average value, conduct comparative research, and judge the strength of its antibacterial ability according to the diameter of the antibacterial zone. The results of the inhibition zone are shown in Table 1.

The bacteriostasis situation of the composition different concentration solutions prepared by table 1 embodiment 1～3

Comparative example 5-8

According to the method of Examples 4-6, the antibacterial experiment was carried out on the traditional Chinese medicine solutions prepared in Comparative Examples 1-4, and the antibacterial zone and antibacterial ability were observed. The results are shown in Table 2.

The bacteriostasis situation of the different concentration solutions of the composition prepared in the comparative example 1～4 of table 2

Examples 7-11

The traditional Chinese medicine compositions prepared in Examples 1-3 were respectively mixed with the grouper basal feed according to the mass ratio of 1.8-2.4:98.2-97.6, and granulated to obtain three groups of medicines.

Each test grouper was challenged with 10 ³ CFU/g of viable Pseudomonas mutans suspension by pleural injection, 0.2 mL of viable Pseudomonas mutans solution. After challenge, each group was fed with basal feed for 40 days to obtain grouper infected with Pseudomonas mutans. Put 20 groupers suffering from Pseudomonas mutans infection into each breeding pond.

Test groups 1 to 3 were fed with the 3 groups of medicines prepared in the above-mentioned examples 1 to 3, and the specific feeding relationship was shown in Table 3. The feeding amount was equivalent to 2% of the grouper body weight in the culture pond as the quality of the traditional Chinese medicine composition. ~3%, the number of times of feeding is 2 times a day, and a total of 40 days of feeding. After the fish is full, suck out the residual bait. Change the water once a day, and the water change volume is 1/3 of the total volume. At the same time, control group Ⅰ (normally fed basal diet, healthy fish) and control group Ⅱ (normally fed basal diet, Pseudomonas mutans challenged fish) were also set up, and each test group and control group were set up with 3 replicate groups. Observe and record the survival every day (observation for 40 days), record the symptoms and death, dissect and check the dead fish, and calculate the cumulative mortality rate (Cumulative mortality rate).

In each group of experiments, if the fish still survived, the above method of feeding was continued until all the fish died. Statistical fish 50%, 100% dead time.

Table 3 Cumulative mortality rate of test groups 1-3 fed with the above-mentioned 3 groups of drugs and control group

Example 12

Prevention and treatment of grouper Pseudomonas metamorphoses by artificial challenge and traditional Chinese medicine:

1, artificial infection experiment: before the experiment starts, the experimental grouper is injected with Pseudomonas mutans to carry out the challenge pre-experiment, more than 80% symptoms occur but the concentration that does not cause death is determined as the experimental bacterium liquid concentration. The oblique-banded grouper was purchased from a farm in Zhangpu City, Fujian Province, with a body length of (11±2) cm, and was temporarily raised in a recirculating aquaculture system of 1 ton of water. They were used for experiments after 1 week of holding. Healthy grouper with similar weight and size were randomly divided into experimental group and control group, with 20 fish in each group, and they were adaptively raised in a recirculating aquaculture system at 18°C for one week. The tested fish were challenged with 10 ³ CFU/g Pseudomonas mutans suspension through pleural injection. The grouper in the control group was injected with PBS buffer. Then transfer the 4 groups of fish into the rearing water body for feeding. Test set control group Ⅰ (feed, healthy fish), control group Ⅱ (feed, Pseudomonas mutans attack poisonous fish), test group Ⅰ (feed plus the traditional Chinese medicine compound prepared in Example 1, deformed pseudomonas Bacteria challenged fish), and compared with the commonly used drugs in production for therapeutic effects, set up control group Ⅲ (feed plus 2.2% Banhuang powder, Pseudomonas proteus challenge fish), control group Ⅳ (feed plus 2.2% % rhubarb galla powder, Pseudomonas mutans challenged fish), each test group and control group were set up 3 repetition groups. After the start of the experiment, they were fed twice a day at 8:00 and 17:00, and the feeding amount was 2% to 3% of the fish body weight, and adjusted according to the growth and feeding conditions. After the fish is full, suck out the residual bait. Change the water once a day, and the water change volume is 1/3 of the total volume. The experimental basal feed was provided by the company. Compound Chinese herbal medicine preparations were added to the test feeds; no additions were made to the control group feeds.

Observe and record the survival every day (observation for 40 days), record the symptoms and death, dissect and check the dead fish, and calculate the cumulative mortality rate (Cumulative mortality rate).

RCM=[(N0-Nt)/N0]×100% formula a

In formula a: RCM is the cumulative mortality rate (%); N0 is the number of fish at the beginning of the test; Nt is the number of fish at the end of the test; relevant index detection is carried out. However, if the fish are still alive, continue to feed them in the above way until all the fish die. Measure the mortality rate (initial fish survival rate, half fish death time, all fish death time).

2. Traditional Chinese medicine treatment of grouper Pseudomonas metamorphoses, the survival rate results of the bait group and the control group

Table 4 shows the mortality of each group after the control group and the experimental group were artificially infected with living bacteria.

Results of the challenge test After 5 days of the challenge test, the grouper groupers in each group died one after another, and the test fish began to show symptoms.

Tests have proved that the death of fish tends to be stable within 40 days after the challenge, so this test records the number of deaths in each group within 40 days after the challenge, and the food intake of the fish in each test group was significantly reduced on the first day and the second day after the challenge. The number of fish deaths was control group Ⅱ > control group Ⅳ > control group Ⅲ > test group Ⅰ > control group Ⅰ.

At the end of the test, the control group I (blank control group, fed with healthy fish) was healthy and alive, with a survival rate of 100.00%, and their activities and food intake were normal, without any visible symptoms.

In the control group Ⅱ, 1 animal died 3 days after the injection of the bacterial suspension, the peak period of death was 14-15 days, and all died (20 animals) on the 17th day.

In the experimental group Ⅰ, 2 animals died 16 days after the injection of the bacterial suspension, and 11 animals died on the 38th day, which was the peak period of death. 53d 100% dead. The 40-day mortality rates of the control group III and IV in the production of commonly used drugs were 85.00% and 95%. The mortality rate was higher than that of this group of prescription drugs.

Table 4 The death situation of the control group and the test group after artificially infecting the grouper with live bacteria

The experimental results show that feeding feed plus traditional Chinese medicine compound can better treat Pseudomonas metamorphoses of the oblique grouper. The therapeutic effect is better than that of Banhuang San and Rhubarb Galla Powder, which are commonly used in the production.

The above is only a preferred embodiment of the present invention, it should be pointed out that, for those of ordinary skill in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, and these improvements and modifications can also be made. It should be regarded as the protection scope of the present invention.

Claims (9)

1. a kind of Chinese medicine composition for treating grouper deformation pseudomonad disease, which is characterized in that including the coptis, Radix Astragali and Huang Cypress；The mass ratio of the coptis, Radix Astragali and Cortex Phellodendri is 0.9~1.3：0.9~1.3：0.9~1.3.

2. Chinese medicine composition according to claim 1, which is characterized in that the mass ratio of the coptis, Radix Astragali and Cortex Phellodendri is 1：1：1.

3. the preparation method of Chinese medicine composition as claimed in claim 1 or 2, which is characterized in that include the following steps：

1) coptis, Radix Astragali and Cortex Phellodendri are crushed, obtains herb powder；

2) herb powder is pulverized, obtains ultramicro medicine powder；

3) ultramicro medicine powder is toasted to 24~48h under the conditions of 60~70 DEG C, obtains Chinese medicine composition.

4. preparation method according to claim 3, which is characterized in that the partial size of herb powder is 140~160 μ in step 1) m；

The partial size of ultramicro medicine powder is 5~10 μm in the step 2).

5. according to the preparation method of claim 3 or 4, which is characterized in that the temperature toasted in the step 3) is 65 DEG C, institute The time for stating baking is 36h.

6. application of the Chinese medicine composition as claimed in claim 1 or 2 in preparation treatment grouper deformation pseudomonad medicine.

7. application according to claim 6, which is characterized in that effective Mlc of the Chinese medicine composition be 60~ 80mg/L。

8. application according to claim 6, which is characterized in that the quality of the Chinese medicine composition accounts for drug gross mass 1.8%~2.4%.

9. application according to any one of claims 6 to 8, which is characterized in that the drug therapy grouper deformation is false The administration time of monad disease is 30~45d.