CN108703154A - Green muscardine fungus kills roach agent and preparation method thereof with boric acid or borax compounding - Google Patents
Green muscardine fungus kills roach agent and preparation method thereof with boric acid or borax compounding Download PDFInfo
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- CN108703154A CN108703154A CN201810889405.3A CN201810889405A CN108703154A CN 108703154 A CN108703154 A CN 108703154A CN 201810889405 A CN201810889405 A CN 201810889405A CN 108703154 A CN108703154 A CN 108703154A
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- roach
- borax
- boric acid
- preparation
- killer
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- 241000231739 Rutilus rutilus Species 0.000 title claims abstract description 74
- 239000004327 boric acid Substances 0.000 title claims abstract description 39
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 title claims abstract description 33
- 235000010339 sodium tetraborate Nutrition 0.000 title claims abstract description 32
- 229910021538 borax Inorganic materials 0.000 title claims abstract description 27
- 239000004328 sodium tetraborate Substances 0.000 title claims abstract description 27
- 241000233866 Fungi Species 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title claims description 28
- 239000003795 chemical substances by application Substances 0.000 title abstract description 18
- 238000013329 compounding Methods 0.000 title abstract description 9
- 239000000843 powder Substances 0.000 claims abstract description 48
- 239000000203 mixture Substances 0.000 claims abstract description 47
- 241000223250 Metarhizium anisopliae Species 0.000 claims abstract description 35
- 239000000470 constituent Substances 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims description 50
- 239000000725 suspension Substances 0.000 claims description 40
- 239000012188 paraffin wax Substances 0.000 claims description 22
- 238000001125 extrusion Methods 0.000 claims description 20
- 230000000694 effects Effects 0.000 claims description 14
- 239000002253 acid Substances 0.000 claims description 3
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 2
- 229910052796 boron Inorganic materials 0.000 claims description 2
- 241000828959 Melolontha hippocastani Species 0.000 claims 1
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- 230000001018 virulence Effects 0.000 abstract description 18
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- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
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- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 2
- 244000000231 Sesamum indicum Species 0.000 description 2
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- 239000000877 Sex Attractant Substances 0.000 description 2
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- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
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- CBOQJANXLMLOSS-UHFFFAOYSA-N ethyl vanillin Chemical compound CCOC1=CC(C=O)=CC=C1O CBOQJANXLMLOSS-UHFFFAOYSA-N 0.000 description 2
- 244000053095 fungal pathogen Species 0.000 description 2
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- QELSKZZBTMNZEB-UHFFFAOYSA-N propylparaben Chemical compound CCCOC(=O)C1=CC=C(O)C=C1 QELSKZZBTMNZEB-UHFFFAOYSA-N 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- IGOWHGRNPLFNDJ-ZPHPHTNESA-N (z)-9-tricosene Chemical compound CCCCCCCCCCCCC\C=C/CCCCCCCC IGOWHGRNPLFNDJ-ZPHPHTNESA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 241000238657 Blattella germanica Species 0.000 description 1
- 241000254173 Coleoptera Species 0.000 description 1
- CIWBSHSKHKDKBQ-DUZGATOHSA-N D-araboascorbic acid Natural products OC[C@@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-DUZGATOHSA-N 0.000 description 1
- YIKYNHJUKRTCJL-UHFFFAOYSA-N Ethyl maltol Chemical compound CCC=1OC=CC(=O)C=1O YIKYNHJUKRTCJL-UHFFFAOYSA-N 0.000 description 1
- 240000008620 Fagopyrum esculentum Species 0.000 description 1
- 235000009419 Fagopyrum esculentum Nutrition 0.000 description 1
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- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
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- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- HYMLWHLQFGRFIY-UHFFFAOYSA-N Maltol Natural products CC1OC=CC(=O)C1=O HYMLWHLQFGRFIY-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 240000000233 Melia azedarach Species 0.000 description 1
- 241000223201 Metarhizium Species 0.000 description 1
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 1
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 235000019483 Peanut oil Nutrition 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
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- 244000062793 Sorghum vulgare Species 0.000 description 1
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- RHDGNLCLDBVESU-UHFFFAOYSA-N but-3-en-4-olide Chemical compound O=C1CC=CO1 RHDGNLCLDBVESU-UHFFFAOYSA-N 0.000 description 1
- 235000014121 butter Nutrition 0.000 description 1
- 235000013736 caramel Nutrition 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- OPGYRRGJRBEUFK-UHFFFAOYSA-L disodium;diacetate Chemical compound [Na+].[Na+].CC([O-])=O.CC([O-])=O OPGYRRGJRBEUFK-UHFFFAOYSA-L 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 235000010350 erythorbic acid Nutrition 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 229940073505 ethyl vanillin Drugs 0.000 description 1
- MJEMIOXXNCZZFK-UHFFFAOYSA-N ethylone Chemical compound CCNC(C)C(=O)C1=CC=C2OCOC2=C1 MJEMIOXXNCZZFK-UHFFFAOYSA-N 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
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- 238000011160 research Methods 0.000 description 1
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- 235000011803 sesame oil Nutrition 0.000 description 1
- 229940083542 sodium Drugs 0.000 description 1
- 235000015424 sodium Nutrition 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017454 sodium diacetate Nutrition 0.000 description 1
- 235000013555 soy sauce Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 230000004763 spore germination Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
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- 239000005720 sucrose Substances 0.000 description 1
- 235000021147 sweet food Nutrition 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 235000012222 talc Nutrition 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- DINKXUCRJBUQAZ-UHFFFAOYSA-N tert-butyl 5-bromopyridine-3-carboxylate Chemical compound CC(C)(C)OC(=O)C1=CN=CC(Br)=C1 DINKXUCRJBUQAZ-UHFFFAOYSA-N 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- IGOWHGRNPLFNDJ-UHFFFAOYSA-N tricos-9t-ene Natural products CCCCCCCCCCCCCC=CCCCCCCCC IGOWHGRNPLFNDJ-UHFFFAOYSA-N 0.000 description 1
- MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 description 1
- 235000019871 vegetable fat Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/002—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits
- A01N25/006—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits insecticidal
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/14—Boron; Compounds thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Pest Control & Pesticides (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Insects & Arthropods (AREA)
- Food Science & Technology (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the biocide containing microbial fungi, insects repellant or attractant technical fields, and in particular to a kind of green muscardine fungus kills roach agent bait formulation with boric acid or borax compounding.The active constituent for killing cockroach bait formulation is compounded by Metarhizium anisopliae CQMa421 conidia powders with boric acid or borax.There is the Roach killer synergistic function, virulence to be enhanced, and the desinsection time is obviously shortened.
Description
Technical field
The invention belongs to contain microbial fungi and boric acid or borax compounding to kill roach agent, and in particular to a kind of green muscardine fungus and boron
Acid or borax compounding kill roach agent bait formulation and preparation method thereof.
Background technology
Cockroach scientific name blattaria also known as croton bug, perfume wife, Shi Ginger, negative disk, talcum, cockroach, block, stove ant machine, red ox
Ox, He Langzi, first by etc.（" China cockroach and its study on prevention are summarized ", Sun Jun etc., the 21st, Beijing Asian-Pacific area manages alliance
Association nd Annual Meeting compilation, the 239-246 pages, publication date on December 31st, 2009）, it is a kind of extremely harmful sanitary insect pest,
It carries multiple pathogens, and to drain contaminated food, utensil, sting damage article, to human health and normal life, study structure
At very big threat（" cockroach prevention and cure progress ", Sun Chenxi, the anti-system of medical faunae, the 1st phase of volume 19 in 2003, the 300-302 pages,
Publication date on May 31st, 2003）.
Chemical agent is the important means of current prevention cockroach, is divided according to the mode of action, can be by the chemistry of anti-cockroach processed
Medicament is divided into medication of tagging, malicious stomach medication and stifling medication.It is killed after insect bodies dermal contact medicament, medication of tagging is actively
Roach is killed, effect is fast, but the roach that goes out is not easy thoroughly, and times for spraying is more, is also easy to produce resistance, influences big, interior to environment and dispenser personnel
Medication has certain safety issue because of stimulation and percutaneous toxicity.Stifling medication has special movement and physicochemical property, be applicable in compared with
The processing of large space desinsection, penetration is strong, easy to use, and drug effect is rapid, can comparatively fast reduce cockroach density, but lasting effect is bad,
It causes serious pollution to the environment；Bait formulation is malicious stomach medication, have lasting medicine, killing rate it is high, it is chain kill roach, be not easy to produce resistance, safety,
Environmentally friendly, hygienic, corrosion-free, application method is simple（" chemical agent and cockroach prevention and cure ", Hua Yilin etc., Chinese hygienic biocide medical instruments,
The 2nd phase of volume 13 in 2007, the 122-123 pages, publication date on December 31st, 2007）.
Insect pathogenic fungus is most important insecticidal microorganism monoid, has specificity, safety, insect will not be made to generate
The advantages that resistance, can form extensive and lasting Pest control efficacy, mainly infect insect by body wall.Green muscardine fungus
（Metarhizium）It is the insect pathogenic fungus for being used for pest control earliest, is utilized to the research of pest control and puts into practice
More century-old.So far, registered green muscardine fungus pesticide product has more than 30 both at home and abroad, including green muscardine fungus kills roach agent.However, existing
Green muscardine fungus kill the shortcomings of roach agent is slower there are desinsection speed, limit its scope of application.
Invention content
In view of this, the purpose of the present invention is to provide a kind of Metarhizium anisopliaes and boric acid or borax compounding to kill cockroach
The virulence of agent, the Roach killer is enhanced, and desinsection speed is improved.
To achieve the above object, the technical scheme is that：
Roach killer, active constituent are compounded by Metarhizium anisopliae CQMa421 conidia powders with boric acid or borax.
Inventor has been surprisingly found that Metarhizium anisopliae CQMa421 conidia powders have with boric acid or borax during the experiment
Synergistic function, active constituent kill cockroach made of being compounded by Metarhizium anisopliae CQMa421 conidia powders and boric acid or borax
The virulence of agent is strong, and desinsection speed is fast.
In order to further enhance the virulence of Roach killer, by percentage to the quality, proportion relation is：Metarhizium anisopliae
CQMa421 conidia powders 1%-20% and boric acid or borax 80%-99%.
For Metarhizium anisopliae CQMa421, the mass percent refers to the matter of Metarhizium anisopliae spore suspension
Measure percentage.
In order to further enhance the virulence of Roach killer, in terms of matter mass percent, proportion relation is：Metarhizium anisopliae
CQMa421 conidia powders 5%-15% and boric acid or borax 85%-95%.
Further include phagostimulant to further enhance the virulence of Roach killer.The phagostimulant includes sweet food agent（Sucrose,
One kind in malt glucide powder, maltose syrups, glucose, fructose, lactose, brown sugar, white granulated sugar, corn sugar residue and sugarcane sugar residue
Or it is a variety of）And cereals（It is one or more in wheat bran, peanut, sesame, corn, buckwheat, oat, rice and millet）.
In order to further enhance the virulence of Roach killer, by percentage to the quality, proportion relation is：Active constituent 5%-25%
With phagostimulant 75%-95%.
Further, by percentage to the quality, proportion relation is：Active constituent 10%-20% and phagostimulant 80%-90%.
In Roach killer according to the present invention, those skilled in the art can also be according to specific requirement, in this hair
Essence is added in bright bait formulation（Furanone, soy sauce ketone, vegetable fat powder, full fat soybean essence, peanut essence, sesame flavor, corn are fragrant
Essence, oat essence, butter essence, Caramel aromatic essence, vanillic aldehyde, Ethyl vanillin, maltol and one kind in ethylmaltol or
A variety of made mixtures）, sex attractant（One or both of cockroach frass corpse and muscalure）, preservative（Benzene first
Sour sodium, potassium sorbate, propylparaben, sodium Diacetate, dehydroactic acid sodium and one kind in D-araboascorbic acid sodium or
It is a variety of）Equal auxiliary agents.Above-mentioned essence, sex attractant and preservative are clear concept for those of ordinary skills.
The virulence of the Roach killer enhances, and the desinsection time is obviously shortened.
The lasting period of the Roach killer is long, has lasting effect to cockroach.
The present invention also aims to protect the preparation method of the Roach killer, include the following steps：
A. oily spore suspension is made in Metarhizium anisopliae CQMa421 conidia powders；
B. the edible paraffin melted boric acid or borax mixes, be cooled to 30 ± 2 DEG C it is spare；
C. phagostimulant addition Metarhizium anisopliae CQMa421 spores suspension is mixed thoroughly, add mixture obtained by step B and stirred
Mix uniformly, the lard of fusing is then added and stirs evenly, extrusion forming postcooling to get.
Further, a concentration of 5,000,000,000 spores/mL of the oily spore suspension.
Further, the dosage of the edible paraffin is edible paraffin and boric acid or the 5%-40% of borax gross mass.
Further, the dosage of the lard is the 10%-15% of mixture gross mass.
The beneficial effects of the present invention are：
The Roach killer of the present invention has synergistic function.
The virulence enhancing of the Roach killer of the present invention, desinsection time are obviously shortened.
The lasting period of the Roach killer of the present invention is long.
The stability of the Roach killer of the present invention.
The preparation method of the Roach killer of the present invention is simple, it is easy to accomplish industrialized production.
Specific implementation mode
Illustrated embodiment is to preferably be illustrated to present disclosure, but is not that present disclosure only limits
In illustrated embodiment.So those skilled in the art carry out nonessential change according to foregoing invention content to embodiment
Into and adjustment, still fall within protection scope of the present invention.
Following boric acid and borax are that analysis is pure；
Following Groton bug and American cockroach come from disinsection fungal pesticide developing engineering center of Chongqing City.
Embodiment 1
Roach killer, preparation method are：
A. 5g Metarhizium anisopliae CQMa421 conidia powders are dispersed in 95g peanut oil and are stirred evenly, filtered with 80 mesh screens,
A concentration of 5% oily spore suspension is made；
B. 85 ± 2 DEG C fusing 50g eat paraffin, be added 45g boric acid mixing, be cooled to 30 ± 2 DEG C it is spare；
C. white sugar 75g, milk powder 75g are weighed and fresh wheat bran 705g is stirred evenly, oily spore suspension obtained by step A is then added and stirs
It mixes uniformly, adds mixture obtained by step B and stir evenly；
D. 100g refining lards are weighed, is then completely melt at a temperature of 80 DEG C and is cooled to 38 ± 2 DEG C at room temperature, add step
Mixture obtained by rapid C simultaneously stirs evenly, extrusion forming postcooling to get.
Embodiment 2
Roach killer, preparation method are：
A. 10g Metarhizium anisopliae CQMa421 conidia powders are dispersed in 90g rapeseed oils and are stirred evenly, filtered with 80 mesh screens,
A concentration of 10% oily spore suspension is made；
B.85 ± 2 DEG C fusing 50g eat paraffin, be added 90g boric acid mixing, be cooled to 30 ± 2 DEG C it is spare；
C. white sugar 75g, milk powder 75g are weighed and fresh wheat bran 650g is stirred evenly, oily spore suspension obtained by step A is then added and stirs
It mixes uniformly, adds mixture obtained by step B and stir evenly；
D. 110g refining lards are weighed, is then completely melt at a temperature of 80 DEG C and is cooled to 38 ± 2 DEG C at room temperature, add step
Mixture obtained by rapid C simultaneously stirs evenly, extrusion forming postcooling to get.
Embodiment 3
Roach killer, preparation method are：
A. 15g Metarhizium anisopliae CQMa421 conidia powders are dispersed in 85g soybean oils and are stirred evenly, filtered with 80 mesh screens,
A concentration of 15% oily spore suspension is made；
B. 85 ± 2 DEG C fusing 50g eat paraffin, be added 135g boric acid mixing, be cooled to 30 ± 2 DEG C it is spare；
C. white sugar 75g, milk powder 75g are weighed and fresh wheat bran 590g is stirred evenly, oily spore suspension obtained by step A is then added and stirs
It mixes uniformly, adds mixture obtained by step B and stir evenly；
D. 125g refining lards are weighed, is then completely melt at a temperature of 80 DEG C and is cooled to 38 ± 2 DEG C at room temperature, add step
Mixture obtained by rapid C simultaneously stirs evenly, extrusion forming postcooling to get.
Embodiment 4：
Roach killer, preparation method are：
A. 40g Metarhizium anisopliae CQMa421 conidia powders are dispersed in 60g sesame oil and are stirred evenly, filtered with 80 mesh screens,
A concentration of 40% oily spore suspension is made；
B. 85 ± 2 DEG C fusing 50g eat paraffin, be added 180g boric acid mixing, be cooled to 30 ± 2 DEG C it is spare；
C. white sugar 75g, milk powder 75g are weighed and fresh wheat bran 500g is stirred evenly, oily spore suspension obtained by step A is then added and stirs
It mixes uniformly, adds mixture obtained by step B and stir evenly；
D. 140g refining lards are weighed, is then completely melt at a temperature of 80 DEG C and room temperature is cooled to 38 ± 2 DEG C, add step C
Gained mixture simultaneously stirs evenly, extrusion forming postcooling to get.
Embodiment 5
Roach killer, preparation method are：
A. 10g Metarhizium anisopliae CQMa421 conidia powders are dispersed in 90g rapeseed oils and are stirred evenly, filtered with 80 mesh screens,
A concentration of 10% oily spore suspension is made；
B. 85 ± 2 DEG C fusing 50g eat paraffin, be added 190g boric acid mixing, be cooled to 30 ± 2 DEG C it is spare；
C. white sugar 75g, milk powder 75g are weighed and fresh wheat bran 510g is stirred evenly, oily spore suspension obtained by step A is then added and stirs
It mixes uniformly, adds mixture obtained by step B and stir evenly；
D. 150g refining lards are weighed, is then completely melt at a temperature of 80 DEG C and room temperature is cooled to 38 ± 2 DEG C, add step C
Gained mixture simultaneously stirs evenly, extrusion forming postcooling to get.
Embodiment 6
Roach killer, preparation method are：
A. 20g Metarhizium anisopliae CQMa421 conidia powders are dispersed in 80g rapeseed oils and are stirred evenly, filtered with 80 mesh screens,
A concentration of 20% oily spore suspension is made；
B. 85 ± 2 DEG C fusing 50g eat paraffin, be added 180g boric acid mixing, be cooled to 30 ± 2 DEG C it is spare；
C. white sugar 75g, milk powder 75g are weighed and fresh wheat bran 520g is stirred evenly, oily spore suspension obtained by step A is then added and stirs
It mixes uniformly, adds mixture obtained by step B and stir evenly；
D. 150g refining lards are weighed, is then completely melt at a temperature of 80 DEG C and room temperature is cooled to 38 ± 2 DEG C, add step C
Gained mixture simultaneously stirs evenly, extrusion forming postcooling to get.
Embodiment 7
Roach killer, preparation method are：
A. 25g Metarhizium anisopliae CQMa421 conidia powders are dispersed in 75g rapeseed oils and are stirred evenly, filtered with 80 mesh screens,
A concentration of 25% oily spore suspension is made；
B. 85 ± 2 DEG C fusing 50g eat paraffin, be added 175g boric acid mixing, be cooled to 30 ± 2 DEG C it is spare；
C. white sugar 75g, milk powder 75g are weighed and fresh wheat bran 525g is stirred evenly, oily spore suspension obtained by step A is then added and stirs
It mixes uniformly, adds mixture obtained by step B and stir evenly；
D. 150g refining lards are weighed, is then completely melt at a temperature of 80 DEG C and room temperature is cooled to 38 ± 2 DEG C, add step C
Gained mixture simultaneously stirs evenly, extrusion forming postcooling to get.
Embodiment 8
Roach killer, preparation method are：
A. Metarhizium anisopliae CQMa421 conidia powders are dispersed in rapeseed oil and are stirred evenly, filtered, be made dense with 80 mesh screens
Degree is the oily spore suspension of 5,000,000,000 spores/mL；
A. 30g Metarhizium anisopliae CQMa421 conidia powders are dispersed in 70g rapeseed oils and are stirred evenly, filtered with 80 mesh screens,
A concentration of 30% oily spore suspension is made；
B. 85 ± 2 DEG C fusing 50g eat paraffin, be added 170g boric acid mixing, be cooled to 30 ± 2 DEG C it is spare；
C. white sugar 75g, milk powder 75g are weighed and fresh wheat bran 530g is stirred evenly, oily spore suspension obtained by step A is then added and stirs
It mixes uniformly, adds mixture obtained by step B and stir evenly；
D. 150g refining lards are weighed, is then completely melt at a temperature of 80 DEG C and room temperature is cooled to 38 ± 2 DEG C, add step C
Gained mixture simultaneously stirs evenly, extrusion forming postcooling to get.
Embodiment 9
Roach killer, preparation method are：
A. 40g Metarhizium anisopliae CQMa421 conidia powders are dispersed in 60g rapeseed oils and are stirred evenly, filtered with 80 mesh screens,
A concentration of 40% oily spore suspension is made；
B. 85 ± 2 DEG C fusing 50g eat paraffin, be added 160g boric acid mixing, be cooled to 30 ± 2 DEG C it is spare；
C. white sugar 75g, milk powder 75g are weighed and fresh wheat bran 540g is stirred evenly, oily spore suspension obtained by step A is then added and stirs
It mixes uniformly, add mixture obtained by step B and stirs evenly；
D. 150g refining lards are weighed, is then completely melt at a temperature of 80 DEG C and room temperature is cooled to 38 ± 2 DEG C, add step C
Gained mixture simultaneously stirs evenly, extrusion forming postcooling to get.
Embodiment 10
Roach killer, preparation method are：
A. 10g Metarhizium anisopliae CQMa421 conidia powders are dispersed in 90g rapeseed oils and are stirred evenly, filtered with 80 mesh screens,
A concentration of 10% oily spore suspension is made；
B. 85 ± 2 DEG C fusing 50g eat paraffin, be added 190g boraxs mixing, be cooled to 30 ± 2 DEG C it is spare；
C. white sugar 75g, milk powder 75g are weighed and fresh wheat bran 510g is stirred evenly, oily spore suspension obtained by step A is then added and stirs
It mixes uniformly, adds mixture obtained by step B and stir evenly；
D. 150g refining lards are weighed, is then completely melt at a temperature of 80 DEG C and room temperature is cooled to 38 ± 2 DEG C, add step C
Gained mixture simultaneously stirs evenly, extrusion forming postcooling to get.
Embodiment 11
Roach killer, preparation method are：
A. 20g Metarhizium anisopliae CQMa421 conidia powders are dispersed in 80g rapeseed oils and are stirred evenly, filtered with 80 mesh screens,
A concentration of 20% oily spore suspension is made；
B. 85 ± 2 DEG C fusing 50g eat paraffin, be added 180g boraxs mixing, be cooled to 30 ± 2 DEG C it is spare；
C. white sugar 75g, milk powder 75g are weighed and fresh wheat bran 520g is stirred evenly, oily spore suspension obtained by step A is then added and stirs
It mixes uniformly, adds mixture obtained by step B and stir evenly；
D. 150g refining lards are weighed, is then completely melt at a temperature of 80 DEG C and room temperature is cooled to 38 ± 2 DEG C, add step C
Gained mixture simultaneously stirs evenly, extrusion forming postcooling to get.
Embodiment 12
Roach killer, preparation method are：
A. 25g Metarhizium anisopliae CQMa421 conidia powders are dispersed in 75g rapeseed oils and are stirred evenly, filtered with 80 mesh screens,
A concentration of 25% oily spore suspension is made；
B. 85 ± 2 DEG C fusing 50g eat paraffin, be added 175g boraxs mixing, be cooled to 30 ± 2 DEG C it is spare；
C. white sugar 75g, milk powder 75g are weighed and fresh wheat bran 525g is stirred evenly, oily spore suspension obtained by step A is then added and stirs
It mixes uniformly, adds mixture obtained by step B and stir evenly；
D. 150g refining lards are weighed, is then completely melt at a temperature of 80 DEG C and room temperature is cooled to 38 ± 2 DEG C, add step C
Gained mixture simultaneously stirs evenly, extrusion forming postcooling to get.
Embodiment 13
Roach killer, preparation method are：
A. Metarhizium anisopliae CQMa421 conidia powders are dispersed in rapeseed oil and are stirred evenly, filtered, be made dense with 80 mesh screens
Degree is the oily spore suspension of 5,000,000,000 spores/mL；
A. 30g Metarhizium anisopliae CQMa421 conidia powders are dispersed in 70g rapeseed oils and are stirred evenly, filtered with 80 mesh screens,
A concentration of 30% oily spore suspension is made；
B. 85 ± 2 DEG C fusing 50g eat paraffin, be added 170g boraxs mixing, be cooled to 30 ± 2 DEG C it is spare；
C. white sugar 75g, milk powder 75g are weighed and fresh wheat bran 530g is stirred evenly, oily spore suspension obtained by step A is then added and stirs
It mixes uniformly, adds mixture obtained by step B and stir evenly；
D. 150g refining lards are weighed, is then completely melt at a temperature of 80 DEG C and room temperature is cooled to 38 ± 2 DEG C, add step C
Gained mixture simultaneously stirs evenly, extrusion forming postcooling to get.
Embodiment 14
Roach killer, preparation method are：
A. 40g Metarhizium anisopliae CQMa421 conidia powders are dispersed in 60g rapeseed oils and are stirred evenly, filtered with 80 mesh screens,
A concentration of 40% oily spore suspension is made；
B. 85 ± 2 DEG C fusing 50g eat paraffin, be added 160g boraxs mixing, be cooled to 30 ± 2 DEG C it is spare；
C. white sugar 75g, milk powder 75g are weighed and fresh wheat bran 540g is stirred evenly, oily spore suspension obtained by step A is then added and stirs
It mixes uniformly, add mixture obtained by step B and stirs evenly；
D. 150g refining lards are weighed, is then completely melt at a temperature of 80 DEG C and room temperature is cooled to 38 ± 2 DEG C, add step C
Gained mixture simultaneously stirs evenly, extrusion forming postcooling to get.
Comparative example 1
Green muscardine fungus cockroach killing bait single dose, preparation method are：
A. 40g Metarhizium anisopliae CQMa421 conidia powders are dispersed in 60g rapeseed oils and are stirred evenly, filtered with 80 mesh screens,
A concentration of 40% oily spore suspension is made；
B. white sugar 75g, milk powder 75g are weighed and fresh wheat bran 650g is stirred evenly, oily spore suspension obtained by step A is then added and stirs
It mixes uniformly；
C. 100g refining lards are weighed, is then completely melt at a temperature of 80 DEG C and room temperature is cooled to 38 ± 2 DEG C, add step C
Gained mixture simultaneously stirs evenly, extrusion forming postcooling to get.
Comparative example 2
Boric acid cockroach killing bait single dose, preparation method are：
A. 85 ± 2 DEG C fusing 50g eat paraffin, be added 100g boric acid mixing, be cooled to 30 ± 2 DEG C it is spare；
B. white sugar 75g, milk powder 75g are weighed and fresh wheat bran 600g is stirred evenly, add mixture obtained by step A and is stirred equal
It is even；
C. 100g refining lards are weighed, is then completely melt at a temperature of 80 DEG C and room temperature is cooled to 38 ± 2 DEG C, add step B
Gained mixture simultaneously stirs evenly, extrusion forming postcooling to get.
Comparative example 3
Borax cockroach killing bait single dose, preparation method are：
A. 85 ± 2 DEG C fusing 50g eat paraffin, be added 100g boraxs mixing, be cooled to 30 ± 2 DEG C it is spare；
B. white sugar 75g, milk powder 75g are weighed and fresh wheat bran 600g is stirred evenly, add mixture obtained by step A and is stirred equal
It is even；
C. 100g refining lards are weighed, is then completely melt at a temperature of 80 DEG C and room temperature is cooled to 38 ± 2 DEG C, add step B
Gained mixture simultaneously stirs evenly, extrusion forming postcooling to get.
Comparative example 4
Blank bait
A. white sugar 75g, milk powder 75g are weighed and fresh wheat bran 700g is stirred evenly, 50g rapeseed oils are then added and stir evenly；
B. 100g refining lards are weighed, is then completely melt at a temperature of 80 DEG C and room temperature is cooled to 38 ± 2 DEG C, add step C
Gained mixture simultaneously stirs evenly, extrusion forming postcooling to get.
Green muscardine fungus-boric acid synergy experiment
Using the oily spore suspension of a concentration of 5,000,000,000 spores/mL as standard agent, toxicity index（TI）It is 100, made from comparative example 2
Roach killer is another single dose, carries out synergistic experiment to Roach killer made from embodiment 5-9, specially：
Using healthy Groton bug as test worm, test worm and each medicament (graded doses 1g, 2g, 3g, 4g, 5g, 6g) are respectively put into life
In measuring tank（1m☓1m☓1m）It is interior, per 30, case, 27 ± 1 DEG C of temperature is adjusted, relative humidity 75%-85%, medicament adds after use
Enter the fresh wheat bran of 10g, replaces daily；Dead borer population and borer population living were counted for 5 days after medicine, calculates the death rate（%）, DPS softwares are used in combination
Count the lethal dose of 50（LD50）, co-toxicity coefficient (CTC) is calculated according to Sun methods (1960),
It is used in combination CTC values to judge the co-toxicity effect of green muscardine fungus-boric acid, CTC<70 be antagonism, and CTC=70-150 is to be added
Effect, the certain synergistic effects of CTC=150-20, CTC>200 be notable synergistic（" Simple Calculation of co-toxicity coefficient and its application ", it is yellow
Attain clearly, the anti-system of medical faunae, the 2nd phase of volume 9 in 1993, the 86-87 pages, publication date on December 31st, 1993）, each group
If 4 repetitions, using its average value as final result, the above each group is in addition to medicament is different, other way to manages are identical, result
As shown in table 1.
1 co-toxicity of table acts on test result
As shown in Table 1, the co-toxicity coefficient of Roach killer made from embodiment 5-9（CTC）For 200.9-293.7, it is all higher than 200.
Thus it proves, Metarhizium anisopliae CQMa421- boric acid complex preparations have notable synergistic effect.
Green muscardine fungus-borax synergy experiment
Using the oily spore suspension of a concentration of 500,000,000 spores/mL as standard agent, toxicity index（TI）It is 100, made from comparative example 3
Roach killer is another single dose, carries out synergistic experiment to Roach killer made from embodiment 10-14, specially：
Using healthy American cockroach as test worm, test worm, each medicament (graded doses 1g, 2g, 3g, 4g, 5g, 6g) are respectively put into raw survey
In case（1m☓1m☓1m）It is interior, per 15, case, 27 ± 1 DEG C of temperature is adjusted, relative humidity 75%-85%, medicament is added after use
The fresh wheat bran of 10g is replaced daily；Dead borer population and borer population living were counted for 7 days after medicine, calculates the death rate（%）, DPS softwares is used in combination to unite
Count the lethal dose of 50（LD50）, co-toxicity coefficient (CTC) is calculated according to Sun methods (1960),
It is used in combination CTC values to judge the co-toxicity effect of green muscardine fungus-boric acid, CTC<70 be antagonism, and CTC=70-150 is to be added
Effect, the certain synergistic effects of CTC=150-20, CTC>200 be notable synergistic（" Simple Calculation of co-toxicity coefficient and its application ", it is yellow
Attain clearly, the anti-system of medical faunae, the 2nd phase of volume 9 in 1993, the 86-87 pages, publication date on December 31st, 1993）, each group
4 repetitions are all provided with, using its average value as final result；For the above each group in addition to medicament is different, other way to manages are identical, knot
Fruit is as shown in table 2.
2 co-toxicity of table acts on test result
As shown in Table 2, the co-toxicity coefficient of Roach killer made from embodiment 10-14（CTC）For 229.1-303.1, it is all higher than
200.Thus it proves, Metarhizium anisopliae CQMa421- borax complex preparations have notable synergistic effect.
Virulence test
Virulence test is carried out to Roach killer made from embodiment 1-5 and comparative example 1-2 respectively, specially：
From in the Groton bug population of indoor raising, healthy adult is selected；Test worm, 5g green muscardine fungus-boric acid are compounded into Roach killer
It is respectively put into raw measuring tank with 5g water（1m☓1m☓1m）It is interior, per 50, case, adjust 27 ± 1 DEG C of temperature, relative humidity 75%-
85%, for 48 hours after medicine, the fresh wheat bran of 10g was replaced daily；It is single with the obtained Roach killer of 5g comparative examples 1 and 5g comparative examples 2
Agent compares, and is blank control with 5g comparative examples 4；For counting dead borer population and borer population living after medicine daily, the death rate is calculated（%）, use
When the DPS software statistics lethal doses of 50（LT50）；Each group is all provided with 4 repetitions, using its average value as final result；It is above each
Group is in addition to medicament is different, other way to manages are identical, and the results are shown in Table 3.
3 virulence test measurement result of table（The death rate, %）
As shown in Table 3, compared with comparative example 1 and comparative example 2, the LT50 of Roach killer made from embodiment 1-5 shortens 1-2 days,
5 days death rates are more than 90% after medicine, and the green muscardine fungus single dose of comparative example 15 days death rate < 50% after medicine, comparative example 2
Boric acid single dose after medicine 5 days death rate < 70%.Thus it proves, Metarhizium anisopliae CQMa421- boric acid compounding kills cockroach
Agent is apparently higher than the virulence of Groton bug the virulence of green muscardine fungus single dose and boric acid single dose, and the desinsection time is obviously shortened.
Virulence test
Virulence test is carried out to Roach killer made from embodiment 10-14 and comparative example 1 and comparative example 3 respectively, specially：
From in the American cockroach population of indoor raising, healthy adult is selected；By test worm, 5g deadlocks bacterium-borax compounding Roach killer and
5g water is respectively put into raw measuring tank（1m☓1m☓1m）It is interior, per 20, case, 27 ± 1 DEG C, relative humidity 75%-85% of temperature is adjusted,
For 48 hours after medicine, the fresh wheat bran of 10g was replaced daily；Using the obtained Roach killer of 5g comparative examples 1 and 5g comparative examples 2 as single dose pair
According to being blank control with 5g comparative examples 4, be repeated 4 times；For the dead borer population of daily statistical observation after medicine and borer population living, the death rate is calculated
（%）, when with the DPS software statistics lethal doses of 50（LT50）, the results are shown in Table 4.
4 toxicity test result of table（The death rate, %）
As shown in Table 4, compared with comparative example 1 and comparative example 3, the LT of Roach killer made from embodiment 10-1450Shorten 1-2
It, 6 days death rates are more than 85% after medicine, and the death in 6 days after medicine of Roach killer made from comparative example 1 and comparative example 3
Rate < 65%.Thus it proves, Metarhizium anisopliae CQMa421- boraxs complex preparation is apparently higher than the virulence of American cockroach green
The virulence of stiff bacterium single dose and borax single dose, desinsection time are obviously shortened.
Storage stability test
Storage stability test is just carried out to killing roach agent agent made from embodiment 1-14 and comparative example 1, specially：
By Roach killer made from embodiment 1-14 and comparative example 1 in room temperature（25℃±1℃）Lower storage, it is true that timing measures desinsection
The germination rate of bacterium spore：5 grams of Roach killers are weighed, the fully dispersed rear filtering of 100mL kerosene is added, takes 50 μ L filtrate even spreads
On 1/4 intensity Sa Shi culture plates, with microscopy after the dyeing of cotton indigo plant after being cultivated 24 hours at a temperature of 26 DEG C, 200-300 are counted
Spore records the spore count of sprouting, calculates germination rate, is used in combination SPSS softwares for statistical analysis；And after 2 months, 4
After month and after 6 months, the spore count of sprouting is recorded, calculates germination rate, it is for statistical analysis with SPSS softwares；Each group is all provided with
4 repetitions, using its average value as final result；For the above each group in addition to medicament is different, other way to manages are identical, as a result such as table
Shown in 5.
5 storage stability test test result of table
As shown in Table 5, after Roach killer made from embodiment 1-14 is stored 6 months, spore germination rate is compared with comparative example 1 without aobvious
Write sex differernce.Thus it proves, with green muscardine fungus compounding Roach killer obtained, the storage for not influencing green muscardine fungus is stablized for boric acid or borax
Property.
Lasting effect is tested
Lasting effect experiment is just carried out to embodiment 1-14 and comparative example 1-3, specially：
It carries out Roach killer made from embodiment 1-14 and comparative example 1-3 to kill cockroach determination of activity：From the Germany of indoor raising
In small Lian populations, using healthy male imago as test worm, it is put into raw measuring tank（1m☓1m☓1m）It is interior, per 50, case；It weighs 5g and kills roach
Medicament and 5g water are respectively put into raw measuring tank by dung beetle agent, adjust 27 ± 1 DEG C of temperature, and the raising of humidity 75%-85% half-lights after 3 days, takes
Go out for trying bait formulation, places into Groton bug male imago 50, the fresh wheat bran raisings of 10g are added, and replace daily, 5 after for medicine
It, count dead borer population amount within 30 days and 60 days, and according to formulaCalculate the death rate, and embodiment 1-9
It is control that Roach killer, which is made, with comparative example 1 and comparative example 2, and embodiment 10-14 is made with comparative example 1 and comparative example 3 and kills cockroach
Agent is control；Each group is all provided with 4 repetitions, using its average value as final result；The above each group in addition to medicament is different, other
Way to manage is identical, and the results are shown in Table 6.
6 duration experimental test result of table
As shown in Table 6, the boric acid of Roach killer medicine and comparative example 2 made from embodiment 1-9,3 days death rates are without notable after medicine
Difference, 30 days, 60 days insecticidal effects be significantly higher than the green muscardine fungus comparative example 1 of comparative example 1.Thus it proves, embodiment 1-9 is made
Roach killer medical instrument have the quick-acting effects of desinsection and a lasting control effect, and continue control effect and be better than green muscardine fungus single dose.
As shown in Table 6, the borax of Roach killer medicine and comparative example 3 made from embodiment 10-14,3 days death rates after medicine
Without significant difference, 30 days, 60 days insecticidal effects be significantly higher than the green muscardine fungus of comparative example 1.Thus it proves, embodiment 10-14 systems
The Roach killer medical instrument obtained has the quick-acting effects of desinsection and lasting control effect, and continues control effect and be better than green muscardine fungus single dose.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiment being appreciated that.
Claims (9)
1. Roach killer, which is characterized in that active constituent is compounded by Metarhizium anisopliae CQMa421 conidia powders and boric acid or borax
It forms.
2. Roach killer according to claim 1, which is characterized in that by percentage to the quality, proportion relation is：Cockchafer
Sub- green muscardine fungus CQMa421 conidia powders 1%-20% and boric acid or borax 80%-99%.
3. Roach killer according to claim 2, which is characterized in that by percentage to the quality, proportion relation is：Chafer
Green muscardine fungus CQMa421 conidia powders 5%-15% and boric acid or borax 85%-95%.
4. according to the Roach killer described in claim 1,2 or/3, which is characterized in that further include phagostimulant.
5. Roach killer according to claim 4, which is characterized in that by percentage to the quality, proportion relation is：Activity at
Divide 5%-25% and phagostimulant 75%-95%.
6. Roach killer according to claim 5, which is characterized in that by percentage to the quality, proportion relation is：Activity at
Divide 10%-20% and phagostimulant 80%-90%.
7. the preparation method of any one of the claim 1-6 Roach killers, which is characterized in that include the following steps：
A. oily spore suspension is made in Metarhizium anisopliae CQMa421 conidia powders；
B. the edible paraffin melted boric acid or borax mixes, be cooled to 30 ± 2 DEG C it is spare；
C. phagostimulant addition Metarhizium anisopliae CQMa421 spores suspension is mixed thoroughly, add mixture obtained by step B and stirred
Mix uniformly, the lard of fusing is then added and stirs evenly, extrusion forming postcooling to get.
8. preparation method according to claim 7, which is characterized in that the dosage of the edible paraffin is edible paraffin and boron
The 5%-40% of acid or borax gross mass.
9. preparation method according to claim 7 or 8, which is characterized in that the dosage of the lard is mixture gross mass
10%-15%.
Priority Applications (1)
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| CN110558333A (en) * | 2019-09-24 | 2019-12-13 | 重庆谷百奥生物研究院有限公司 | Composition for killing prodenia litura by metarhizium anisopliae and metaflumizone |
| CN110583700A (en) * | 2019-09-26 | 2019-12-20 | 山东师范大学 | Biological source insecticidal composition and application thereof |
| WO2023111330A1 (en) * | 2021-12-17 | 2023-06-22 | Danstar Ferment Ag | Beneficial acids for enhancing biocontrol efficacy of fungal spores |
| EP4480314A1 (en) * | 2023-06-21 | 2024-12-25 | Danstar Ferment Ag | Beneficial salts for enhancing biocontrol efficacy of fungal spores |
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| CN110558333A (en) * | 2019-09-24 | 2019-12-13 | 重庆谷百奥生物研究院有限公司 | Composition for killing prodenia litura by metarhizium anisopliae and metaflumizone |
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| WO2023111330A1 (en) * | 2021-12-17 | 2023-06-22 | Danstar Ferment Ag | Beneficial acids for enhancing biocontrol efficacy of fungal spores |
| EP4480314A1 (en) * | 2023-06-21 | 2024-12-25 | Danstar Ferment Ag | Beneficial salts for enhancing biocontrol efficacy of fungal spores |
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