CN108653811A - A kind of artificial ligament and preparation method thereof - Google Patents
A kind of artificial ligament and preparation method thereof Download PDFInfo
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- CN108653811A CN108653811A CN201710192757.9A CN201710192757A CN108653811A CN 108653811 A CN108653811 A CN 108653811A CN 201710192757 A CN201710192757 A CN 201710192757A CN 108653811 A CN108653811 A CN 108653811A
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- 210000003041 ligament Anatomy 0.000 title claims abstract description 85
- 238000002360 preparation method Methods 0.000 title claims abstract description 28
- 102000008186 Collagen Human genes 0.000 claims abstract description 49
- 108010035532 Collagen Proteins 0.000 claims abstract description 49
- 229920001436 collagen Polymers 0.000 claims abstract description 47
- 239000002121 nanofiber Substances 0.000 claims abstract description 20
- 238000010041 electrostatic spinning Methods 0.000 claims abstract description 14
- 239000011265 semifinished product Substances 0.000 claims abstract description 13
- 238000009987 spinning Methods 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 9
- 239000002904 solvent Substances 0.000 claims abstract description 7
- 238000003756 stirring Methods 0.000 claims abstract description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 21
- 239000004744 fabric Substances 0.000 claims description 15
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 14
- 238000004132 cross linking Methods 0.000 claims description 9
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 6
- 230000005684 electric field Effects 0.000 claims description 6
- 239000008213 purified water Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000004804 winding Methods 0.000 claims description 6
- 102000001187 Collagen Type III Human genes 0.000 claims description 5
- 108010069502 Collagen Type III Proteins 0.000 claims description 5
- 229920002521 macromolecule Polymers 0.000 claims description 4
- 239000002861 polymer material Substances 0.000 claims description 4
- 239000000047 product Substances 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 238000004513 sizing Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 2
- 239000003292 glue Substances 0.000 claims 2
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 abstract description 7
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 abstract description 7
- 210000002744 extracellular matrix Anatomy 0.000 abstract description 7
- 230000006378 damage Effects 0.000 abstract description 4
- 201000010099 disease Diseases 0.000 abstract description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 3
- 230000002349 favourable effect Effects 0.000 abstract description 3
- 239000011664 nicotinic acid Substances 0.000 abstract description 3
- 230000008929 regeneration Effects 0.000 abstract description 3
- 238000011069 regeneration method Methods 0.000 abstract description 3
- 210000001519 tissue Anatomy 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 28
- 229920000139 polyethylene terephthalate Polymers 0.000 description 5
- 239000005020 polyethylene terephthalate Substances 0.000 description 5
- 210000002435 tendon Anatomy 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 238000013019 agitation Methods 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002070 nanowire Substances 0.000 description 2
- -1 polyethylene terephthalate Polymers 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 1
- 206010060820 Joint injury Diseases 0.000 description 1
- 208000005137 Joint instability Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 101710172711 Structural protein Proteins 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000010907 mechanical stirring Methods 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000013456 study Methods 0.000 description 1
- 230000010148 water-pollination Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/24—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/10—Materials or treatment for tissue regeneration for reconstruction of tendons or ligaments
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Biophysics (AREA)
- Prostheses (AREA)
- Materials For Medical Uses (AREA)
Abstract
The present invention provides a kind of artificial ligaments and preparation method thereof, and preparation method includes the following steps：S1.1：Collagen is dissolved in solvent, stirs and for use solution is made；S1.2：It uses electrostatic spinning technique to carry out spinning on the surface of artificial ligament semi-finished product to form collagen layers of nanofibers for use solution, obtains the artificial ligament.Artificial ligament provided by the invention has collagen layers of nanofibers, makes the outer surface bionic extracellular matrix of artificial ligament, to increase the biocompatibility of artificial ligament, promotes the Regeneration and Repair of disease damage tissue.And preparation method is easy to operate, favorable repeatability, high financial profit.
Description
Technical field
The invention belongs to medical instruments field, it is related to a kind of artificial ligament and preparation method thereof.
Background technology
Cruciate ligament is a kind of clinically common joint injury, typically as caused by violent joint twisting,
Malpractice can lead to joint instability, cause joint secondary lesion, to influence the performance in joint.Operative reconstruction is that intersection is tough
Unique selection with damage, Heterogeneous tendon, self tendon and artificial ligament are all the optional substitutes for damaging ligament.Due to
The rejection of Heterogeneous tendon and self tendon carry out source problem, and in Cruciate ligament reconstruction treatment at present, artificial ligament makes
With more and more extensive.Wherein, LARS artificial ligaments are developed by French Laboureau companies, are started in 1992 for clinic,
Material is pure polyethylene terephthalate (PET).The country, LARS artificial ligaments began to use so far from 2004, but also
The product for a independent development do not occur obtains the certification of officina of state (CFDA).
LARS artificial ligaments are with polyethylene terephthalate (PET) for raw material.But the hydrophily of PET and life
Object compatibility is poor, and rejection is easy tod produce after implanting, and so as to cause inflammation, influences the quality of life of sufferer.Cause
This needs to carry out surface modification to artificial ligament in manufacturing artificial ligament, to increase its biocompatibility, reduces implantation
Rejection after in vivo.
Invention content
The purpose of the present invention is to provide a kind of artificial ligaments and preparation method thereof, to reduce artificial ligament in the prior art
The rejection generated after implanting.
In order to solve the above technical problems, the present invention provides a kind of preparation method of artificial ligament, include the following steps：
S1.1：Collagen is dissolved in solvent, stirs and for use solution is made；
S1.2：Electrostatic spinning technique is used to carry out spinning on the surface of artificial ligament semi-finished product with shape for use solution
At collagen protein nano fibrous layer, to obtain the artificial ligament.
Optionally, in the S1.2, after forming the collagen layers of nanofibers, also to the collagen Nanowire
It ties up layer and carries out crosslinking Treatment.
Optionally, in the S1.1, the collagen is selected from water-soluble I types, II types or type III collagen.
Optionally, in the S1.1, the mass fraction of collagen is 0.8%-16% in for use solution.
Optionally, in the S1.2, the condition of electrostatic spinning is：Spinning voltage is 8-50kV；Electric field distance is 50-
220mm；The flow velocity of solution is 1.0-5.0ml/h.
Optionally, in the S1.1, the solvent is selected from aqueous acetic acid, aqueous hydrochloric acid solution or purified water, the acetic acid
The volume fraction of aqueous solution and the aqueous hydrochloric acid solution is 0.3%-5%.
Further, in the S1.2, the artificial ligament semi-finished product are prepared in accordance with the following steps：
S7.1：Macromolecule polymer material is woven, artificial ligament braided fabric is obtained；
S7.2：By the artificial ligament braided fabric successively by winding, suture, fixed, sizing, cleaning and drying and processing,
Obtain the artificial ligament.
Optionally, in the S1.2, it is the glutaraldehyde solution steam of 20%-40% to the collagen to use mass volume ratio
Protein nano fibrous layer is crosslinked, crosslinking time 12-48h.
The present invention also provides a kind of artificial ligament, the artificial ligament includes collagen layers of nanofibers.
A kind of artificial ligament is prepared using above-mentioned preparation method.
Compared with prior art, artificial ligament provided by the invention includes collagen layers of nanofibers, makes artificial ligament
Outer surface bionic extracellular matrix promote the Regeneration and Repair of disease damage tissue to increase the biocompatibility of artificial ligament.
And preparation method is easy to operate, favorable repeatability, high financial profit.
Description of the drawings
Fig. 1 is a kind of flow diagram of the preparation method of artificial ligament proposed by the present invention.
Specific implementation mode
A kind of artificial ligament proposed by the present invention and preparation method thereof is made into one below in conjunction with the drawings and specific embodiments
Step is described in detail.According to following explanation and claims, advantages and features of the invention will become apparent from.It should be noted that attached
Figure is using very simplified form, only for the purpose of facilitating and clarifying the purpose of the embodiments of the invention.
Applicant it has been investigated that, artificial ligament surface introduce active layer, be the one kind being modified to its surface
Effective method.For avoid artificial ligament be implanted into human body after easy to produce rejection, prepare active layer raw material needs be
Good biocompatibility, it is desirable to similar with the ingredient of natural extracellular matrix.Collagen is a kind of natural material, it is
A kind of structural proteins of extracellular matrix are the main components of extracellular matrix.And applicant have further found that electrostatic spinning skill
Art is to prepare a kind of effective method of nanofibrous structures material, and the nanofibrous structures material prepared using this technology can
To simulate the structure of extracellular matrix well.Currently, there are no correlative studys to disclose using electrostatic spinning technique artificial
It is tough to take the technical solution for preparing collagen layer.
The present invention provides a kind of artificial ligament and preparation method thereof, the artificial ligament surface includes that collagen is received
Rice fibrous layer, the collagen layers of nanofibers are formed using the technology of electrostatic spinning on the surface of artificial ligament.
Specifically, the preparation method of artificial ligament provided by the invention, includes the following steps：
S1.1：Collagen is dissolved in solvent, for use solution is stirred to get；
S1.2：Electrostatic spinning technique is used to carry out spinning on the surface of artificial ligament semi-finished product with shape for use solution
At collagen protein nano fibrous layer, the artificial ligament is obtained.
It, can also be to the collagen Nanowire after forming the collagen layers of nanofibers in the S1.2
Dimension layer is crosslinked, and can increase the stability of collagen layers of nanofibers by crosslinking.Optionally, use mass volume ratio for
The glutaraldehyde solution steam of 20%-40% is crosslinked the collagen layers of nanofibers, crosslinking time 12-48h.
In the S1.1, the collagen can be selected from water-soluble I types, II types or type III collagen, the collagen
The source of albumen can be ox source, pig source, fish source or mouse source.The mass fraction of collagen can be in the solution
0.8%-16%.
Further, in the S1.2, the condition of electrostatic spinning is：Spinning voltage is 8-50kV；Electric field distance is 50-
220mm；The flow velocity of solution is 1.0-5.0ml/h.In the S1.1, the solvent be selected from aqueous acetic acid, aqueous hydrochloric acid solution or
The volume fraction of purified water, the aqueous acetic acid and the aqueous hydrochloric acid solution is 0.3%-5%.
The artificial ligament semi-finished product in the S1.2 can be prepared in accordance with the following steps：
S7.1：Macromolecule polymer material is woven, artificial ligament braided fabric is obtained；
S7.2：By the artificial ligament braided fabric successively by winding, suture, fixed, sizing, cleaning and drying and processing,
Obtain the artificial ligament semi-finished product.
In the above scheme, the S7.2 specifically comprises the following steps：
The artificial ligament braided fabric is cut according to the size of regulation, then along the length side of the artificial ligament
It is wound to from both ends to center, obtains the sample of winding；
By the sample suture of the winding from being sutured to both ends at free-fiber, after being seamed to end, twined with suture
Around fixation, the braided fabric that is fixed；
By the braided fabric fixed with being suitble to the stainless steel tube of artificial ligament length to carry out thermal finalization, obtain stereotyped
Braided fabric；
The stereotyped braided fabric is washed by formula successively, alcohol wash with purified water clean, then dry, obtain institute
State artificial ligament semi-finished product.
Preferably, the fracture tensile strength of the artificial ligament semi-finished product is more than 4000N, and torsion and wear test reach
2000000 times unbroken.
Carry out the artificial ligament of the collagen layers of nanofibers to the present invention with specific embodiment in further detail below
And preparation method thereof be further described, to highlight the features of the present invention and feature.
Embodiment 1
In the present embodiment, collagen is selected from water-soluble I type albumen, and source is fish source, band collagen nanofiber
The preparation method of the artificial ligament of layer is specific as follows：
1.1：Macromolecule polymer material is woven, artificial ligament braided fabric is obtained.
1.2：The artificial ligament braided fabric is cut according to the size of product, then along its length from both ends to
Center is wound；It is solid with thread entanglement after being seamed to end by the sample suture of winding from being sutured to both ends at free-fiber
It is fixed；Then the braided fabric fixed is subjected to thermal finalization with the stainless steel tube of suitable artificial ligament length；Stereotyped braided fabric
Washed successively by formula, alcohol is washed and is cleaned with purified water, then dry, obtain artificial ligament semi-finished product, the artificial ligament half at
The fracture tensile strength of product be more than 4000N, torsion and wear test reach 2,000,000 times it is unbroken.
1.3：Suitable I-type collagen is taken to be dissolved in 5% aqueous acetic acid (volume fraction 5%), it is then mechanical
Stirring, obtains uniform for use solution, wherein the mass fraction of collagen is 0.8% in for use solution.
1.4：Above-mentioned collagen solution is spun using electrostatic spinning technique on the surface of artificial ligament semi-finished product
Silk, to form collagen layers of nanofibers, the condition of electrostatic spinning is：Spinning voltage is 8kV, electric field distance 50mm, molten
The flow velocity of liquid is 1.0ml/h.
1.5：It is 20% glutaraldehyde solution steam to the collagen nanofiber of above-mentioned formation to use mass volume ratio
Layer is crosslinked, crosslinking time 12h, obtains the artificial ligament with the collagen layers of nanofibers stablized.
Embodiment 2
The preparation method of artificial ligament is substantially the same manner as Example 1 in the present embodiment, is retouched below only for difference
It states.
In the present embodiment, collagen is selected from II collagen types, source Niu Laiyuan.
Above-mentioned steps 1.3 are specially：Suitable II collagen types are taken to be dissolved in 0.3% aqueous hydrochloric acid solution (volume fraction
In 0.3%), then mechanical agitation, obtains uniform for use solution, wherein the mass fraction of collagen in for use solution
It is 16%.
In above-mentioned steps 1.4, the condition of electrostatic spinning is：Spinning voltage is 50kV, electric field distance 220mm, solution
Flow velocity is 5.0ml/h.
In above-mentioned steps 1.5, it is 40% glutaraldehyde solution steam to the collagen egg of above-mentioned formation to use mass volume ratio
White layers of nanofibers is crosslinked, and crosslinking time 48h obtains the artificial ligament with the biosphere stablized.
Embodiment 3
The preparation method of artificial ligament is substantially the same manner as Example 1 in the present embodiment, is retouched below only for difference
It states.
In the present embodiment, collagen is selected from type III collagen, and source is pig source.
Above-mentioned steps 1.3 are specially：Suitable type III collagen is taken to be dissolved in purified water, then mechanical agitation, obtains
Uniform for use solution, wherein the mass fraction of collagen is 10% in for use solution.
In above-mentioned steps 1.4, the condition of electrostatic spinning is：Spinning voltage is 30kV, electric field distance 150mm, solution
Flow velocity is 3.0ml/h.
In above-mentioned steps 1.5, it is 30% glutaraldehyde solution steam to the collagen egg of above-mentioned formation to use mass volume ratio
White layers of nanofibers is crosslinked, and crosslinking time is for 24 hours, to obtain the artificial ligament with the biosphere stablized.
The artificial ligament that the method that above-described embodiment provides through the invention is prepared has collagen nanofiber
Layer, makes the outer surface bionic extracellular matrix of artificial ligament, to increase the biocompatibility of artificial ligament, promotes disease damage group
The Regeneration and Repair knitted.And preparation method is easy to operate, favorable repeatability, high financial profit.
Each embodiment is described by the way of progressive in this specification, the highlights of each of the examples are with other
The difference of embodiment, just to refer each other for identical similar portion between each embodiment.
Foregoing description is only the description to present pre-ferred embodiments, not to any restriction of the scope of the invention, this hair
Any change, the modification that the those of ordinary skill in bright field does according to the disclosure above content, belong to the protection of claims
Range.
Claims (10)
1. a kind of preparation method of artificial ligament, which is characterized in that include the following steps：
S1.1：Collagen is dissolved in solvent, stirs and for use solution is made；
S1.2：Electrostatic spinning technique is used to carry out spinning on the surface of artificial ligament semi-finished product to form glue for use solution
Former protein nano fibrous layer, to obtain the artificial ligament.
2. the preparation method of artificial ligament according to claim 1, which is characterized in that in the S1.2, form the glue
After former protein nano fibrous layer, crosslinking Treatment also is carried out to the collagen layers of nanofibers.
3. the preparation method of artificial ligament according to claim 1, which is characterized in that in the S1.1, the collagen egg
It is selected from water-soluble I types, II types or type III collagen in vain.
4. the preparation method of artificial ligament according to claim 1, which is characterized in that described for use molten in the S1.1
The mass fraction of collagen is 0.8%-16% in liquid.
5. the preparation method of artificial ligament according to claim 1, which is characterized in that in the S1.2, electrostatic spinning
Condition is：Spinning voltage is 8-50kV；Electric field distance is 50-220mm；The flow velocity of solution is 1.0-5.0ml/h.
6. the preparation method of artificial ligament according to claim 1, which is characterized in that in the S1.1, the solvent choosing
From aqueous acetic acid, aqueous hydrochloric acid solution or purified water, the volume fraction of the aqueous acetic acid and the aqueous hydrochloric acid solution is
0.3%-5%.
7. the preparation method of artificial ligament according to claim 1, which is characterized in that described artificial tough in the S1.2
Band semi-finished product are prepared in accordance with the following steps：
S7.1：Macromolecule polymer material is woven, artificial ligament braided fabric is obtained；
S7.2：By the artificial ligament braided fabric successively by winding, suture, fixed, sizing, cleaning and drying and processing, obtain
The artificial ligament semi-finished product.
8. the preparation method of artificial ligament according to claim 2, which is characterized in that in the S1.2, using mass body
Product is crosslinked the collagen layers of nanofibers than the glutaraldehyde solution steam for 20%-40%, crosslinking time 12-
48h。
9. a kind of artificial ligament, which is characterized in that the artificial ligament includes collagen layers of nanofibers.
10. a kind of artificial ligament, which is characterized in that be prepared using such as claim 1-8 any one of them preparation methods.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710192757.9A CN108653811B (en) | 2017-03-28 | 2017-03-28 | Artificial ligament and preparation method thereof |
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| CN201710192757.9A CN108653811B (en) | 2017-03-28 | 2017-03-28 | Artificial ligament and preparation method thereof |
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| CN108653811A true CN108653811A (en) | 2018-10-16 |
| CN108653811B CN108653811B (en) | 2021-03-12 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110331486A (en) * | 2019-07-25 | 2019-10-15 | 东华大学 | A kind of multilayered structure nano-fibre yams knitting tendon scaffold and its preparation and application |
| TWI692559B (en) * | 2019-06-03 | 2020-05-01 | 財團法人紡織產業綜合研究所 | Fiber with cell affinity and fiber product made using the same |
| CN112121232A (en) * | 2020-09-17 | 2020-12-25 | 浙江理工大学 | Preparation method of multi-layer composite nano-micro fiber topological morphology scaffold with ECM-like structure |
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| US20020090725A1 (en) * | 2000-11-17 | 2002-07-11 | Simpson David G. | Electroprocessed collagen |
| CN1777450A (en) * | 2003-01-17 | 2006-05-24 | 拉思公司-科学研究与应用试验室 | Biomimetic prosthetic ligament and production method thereof |
| CN101214393A (en) * | 2007-12-28 | 2008-07-09 | 苏州大学 | Nanofiber tissue engineering blood vessel and preparation method thereof |
| CN201154005Y (en) * | 2007-12-21 | 2008-11-26 | 南京大学医学院附属鼓楼医院 | medical knee cruciate ligament |
| CN201529284U (en) * | 2009-09-28 | 2010-07-21 | 中国人民解放军第四军医大学 | A fibrous structure for artificial ligaments |
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| CN104606713A (en) * | 2015-01-04 | 2015-05-13 | 浙江大学 | Three-dimensional parallel collagenous fiber-silk bracket as well as preparation method and application thereof |
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| US20020090725A1 (en) * | 2000-11-17 | 2002-07-11 | Simpson David G. | Electroprocessed collagen |
| CN1777450A (en) * | 2003-01-17 | 2006-05-24 | 拉思公司-科学研究与应用试验室 | Biomimetic prosthetic ligament and production method thereof |
| CN201154005Y (en) * | 2007-12-21 | 2008-11-26 | 南京大学医学院附属鼓楼医院 | medical knee cruciate ligament |
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| CN102908211A (en) * | 2012-10-15 | 2013-02-06 | 复旦大学附属华山医院 | Integral annular suspension artificial ligament |
| CN104606713A (en) * | 2015-01-04 | 2015-05-13 | 浙江大学 | Three-dimensional parallel collagenous fiber-silk bracket as well as preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI692559B (en) * | 2019-06-03 | 2020-05-01 | 財團法人紡織產業綜合研究所 | Fiber with cell affinity and fiber product made using the same |
| CN110331486A (en) * | 2019-07-25 | 2019-10-15 | 东华大学 | A kind of multilayered structure nano-fibre yams knitting tendon scaffold and its preparation and application |
| CN112121232A (en) * | 2020-09-17 | 2020-12-25 | 浙江理工大学 | Preparation method of multi-layer composite nano-micro fiber topological morphology scaffold with ECM-like structure |
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| CN108653811B (en) | 2021-03-12 |
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