CN108444917B - Self-calibrating dim light detection device - Google Patents
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- CN108444917B CN108444917B CN201810569761.7A CN201810569761A CN108444917B CN 108444917 B CN108444917 B CN 108444917B CN 201810569761 A CN201810569761 A CN 201810569761A CN 108444917 B CN108444917 B CN 108444917B
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- 238000001514 detection method Methods 0.000 title claims abstract description 51
- 230000003287 optical effect Effects 0.000 claims abstract description 56
- 230000000903 blocking effect Effects 0.000 claims description 10
- 239000013307 optical fiber Substances 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 description 24
- 239000000376 reactant Substances 0.000 description 8
- 230000000694 effects Effects 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- ORILYTVJVMAKLC-UHFFFAOYSA-N adamantane Chemical compound C1C(C2)CC3CC1CC2C3 ORILYTVJVMAKLC-UHFFFAOYSA-N 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 4
- 238000003384 imaging method Methods 0.000 description 4
- 238000003018 immunoassay Methods 0.000 description 3
- 206010036618 Premenstrual syndrome Diseases 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 230000003595 spectral effect Effects 0.000 description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 208000033999 Device damage Diseases 0.000 description 1
- -1 acridine ester Chemical class 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Natural products C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- RXNXLAHQOVLMIE-UHFFFAOYSA-N phenyl 10-methylacridin-10-ium-9-carboxylate Chemical compound C12=CC=CC=C2[N+](C)=C2C=CC=CC2=C1C(=O)OC1=CC=CC=C1 RXNXLAHQOVLMIE-UHFFFAOYSA-N 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
- G01N21/274—Calibration, base line adjustment, drift correction
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention relates to a self-calibrating dim light detection device, comprising: the box body comprises a front chamber, a detection chamber and a reference chamber; the signal optical system comprises a signal light collector and a photon counting module, wherein the signal light collector is arranged in the front chamber, and the photon counting module is arranged in the detection chamber; the reference optical system is used for calibrating the signal optical system and comprises a diffuse reflection cavity, a reference light source and a photoelectric detector which are arranged in the diffuse reflection cavity, a photoelectric control board which is electrically connected with the reference light source and the photoelectric detector, and a diaphragm which is communicated with the photon counting module and the diffuse reflection cavity, wherein the photoelectric control board is electrically connected with the photon counting module; the diffuse reflection cavity, the reference light source, the photoelectric detector and the photoelectric control panel are arranged in the reference chamber, and the diaphragm is communicated with the detection chamber and the reference chamber. The device has the characteristics of being capable of well improving the stability of calibration, simple in structure and saving cost on the basis of guaranteeing the stability and the performance of the stability.
Description
Technical Field
The invention relates to the technical field of chemiluminescent immunoassay equipment, in particular to a self-calibration dim light detection device.
Background
The chemiluminescent immunoassay belongs to the field of weak light detection, and the temperature change of the environment often causes the weak light detection device to generate temperature drift and other problems, so that the response of the weak light detection device to the same standard signal light is inconsistent. In addition, the response of the weak light detection device may also change due to aging of the device. In order to solve the problems of temperature drift, aging and the like, the traditional weak light detection device mostly adopts a complex calibration light path system, such as various reflectors, spectroscopes, optical filters and the like, some calibration optical systems adopt a plurality of plane reflectors, the angle change of reflected light is 2 times of the angle change of the reflectors, for the weak light detection device, the tiny change of the weak light detection device can generate larger change of photon number, which directly affects the accuracy of calibration, and the device has complex structure, high cost, high assembly and debugging requirements and low mass production efficiency, and the complex structure increases the risk coefficient of instability.
Disclosure of Invention
Based on this, it is necessary to provide a self-calibrating weak light detection device, which has the characteristics of improving the calibration stability well, having a simple structure, ensuring the stability and saving the cost on the basis of the performance thereof.
A self-calibrating dim light detection device, comprising:
the box body comprises a reference chamber, a prepositive chamber and a detection chamber communicated with the prepositive chamber;
The signal optical system comprises a signal light collector and a photon counting module, the signal light collector and the photon counting module are sequentially arranged along the emitting direction of the signal light, the signal light collector is arranged in the front chamber, and the photon counting module is arranged in the detection chamber; and
The reference optical system is used for calibrating the signal optical system and comprises a diffuse reflection cavity, a reference light source and a photoelectric detector which are arranged in the diffuse reflection cavity, a photoelectric control board which is electrically connected with the reference light source and the photoelectric detector, and a diaphragm which is communicated with the photon counting module and the diffuse reflection cavity, wherein the photoelectric control board is electrically connected with the photon counting module; the diffuse reflection cavity, the reference light source, the photoelectric detector and the photoelectric control panel are arranged in the reference chamber, the diaphragm is communicated with the detection chamber and the reference chamber, and light of the reference light source enters the signal optical system through the diaphragm after being diffusely reflected in the diffuse reflection cavity and is projected to the photon counting module.
According to the self-calibration weak light detection device, when in detection, the box body is communicated with the reading chamber to realize sealing, external light cannot penetrate into the box body, and interference of external light is avoided; the output light intensity of the reference light source can be controlled through the photoelectric control panel, the light emitted by the reference light source is subjected to diffuse reflection in the diffuse reflection cavity, part of the light is detected by the photoelectric detector, and a small part of the light is projected to the photon counting module through the diaphragm and is detected by the photon counting module; the light signal of the reference light source with specific light intensity passing through the diaphragm can be quantified, the light signal passing through the diaphragm is not interfered by external factors, the light signal received by the photoelectric detector is in direct proportion to the light signal received by the photon counting module passing through the diaphragm, and thus the light signal received by the photon counting module of the reference light source can be indirectly and accurately measured through the photoelectric detector; the ratio of the light signal received by the photoelectric detector of the reference light source with X light intensity to the light signal received by the photon counting module of the transmission diaphragm before the non-drifting is set as S0, after the photon counting module is subjected to temperature drifting, the output light intensity of the reference light source is controlled to be X light intensity through the photoelectric control panel, the ratio of the light signal received by the photoelectric detector to the light signal received by the photon counting module of the transmission diaphragm is measured as S1, the ratio is compared with the S0 before the non-drifting to obtain a calibration factor F, when a reactant is actually measured, the photon counting module is compensated according to the calibration factor F, namely the actually measured light signal is calibrated and calculated, the reactant light signal in the accurate reaction container is obtained, and the self-calibration effect is achieved; the self-calibration output light is diffuse reflection light, not direct light, so that the stability of the calibration light source can be improved well, the structure is simple, and the cost of the device is saved greatly on the basis of ensuring the stability and the performance of the device.
In one embodiment, a light blocking device and a light transmission channel are arranged in the diffuse reflection cavity, and light of the reference light source enters the diaphragm through the light transmission channel.
In one of the embodiments, the diameter of the diaphragm is 1-10 mm.
In one embodiment, the diffuse reflective cavity is a square cavity.
In one embodiment, the reference light source and the photodetector are mounted on the same side of the diffuse reflecting cavity, and the diaphragm is located on the opposite side of the reference light source.
In one embodiment, the reference light source is an LED lamp or an OLED lamp.
In one embodiment, the photodetector is a photodiode or an avalanche photodiode.
In one embodiment, the signal light collector is a single convex lens.
In one embodiment, the photon counting module includes a photon counting plate and a detector mounted to the photon counting plate.
In one embodiment, the detector is a photomultiplier tube.
Drawings
FIG. 1 is a schematic diagram of a self-calibrating dim light detection device according to a preferred embodiment of the present invention;
FIG. 2 is a cross-sectional view of the self-calibrating dim light detection device according to FIG. 1, taken along the direction A-A;
FIG. 3 is a partial schematic view of a reference-containing optical system in the self-calibrating dim light detection device of FIG. 2;
FIG. 4 is an enlarged schematic view of portion B of the self-calibrating dim light detection device of FIG. 2.
Detailed Description
The present invention will be described more fully hereinafter in order to facilitate an understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
Referring to fig. 1 to 4, a self-calibrating dim light detection device 100 according to a preferred embodiment of the present invention is applied to chemiluminescent immunoassay, and the self-calibrating dim light detection device 100 is optically connected to a reaction vessel capable of generating an optical signal in a reading chamber. In one embodiment, once the reaction vessel enters the reading chamber, a shutter disposed adjacent to the reaction vessel is closed, shielding ambient light around the reaction vessel. In one embodiment, the reaction vessel is disposed on a hole of the tray and is rotatable with the tray, the tray is disposed in a housing, and the tray and the housing together form a reading chamber. During reading, the reaction container rotates to a reading station along with the tray body. It will be appreciated by those skilled in the art that the optical signal within the reaction vessel is generated by a chemiluminescent reaction of reactants within the reaction vessel, such as a chemiluminescent reaction of the directly luminescent acridine ester in the corresponding pre-excitation and excitation solutions, a chemiluminescent reaction of the chemiluminescent substrate, spiral adamantane and derivatives thereof, under alkaline phosphatase catalysis, and the like.
The self-calibration weak light detection device 100 comprises a box 10, a signal optical system 20 and a reference optical system 30, wherein the signal optical system 20 and the reference optical system 30 are installed in the box 10, the box 10 is mainly used for shielding external environment light, the signal optical system 20 is used for collecting signal light and converting the signal light into an electric signal, the reference optical system 30 provides a standard light source for calibration, and the standard light source is used for drift calibration of the signal optical system 20 so as to adjust the detection result, so that the detection result is more accurate.
As shown in fig. 2, the housing 10 includes a pre-chamber 11, a detection chamber 12 communicating with the pre-chamber 11, and a reference chamber 13 communicating with the signal optical system 20, the pre-chamber 11 being adjacent to and communicating with a reading chamber (not shown).
As shown in fig. 2, the signal optical system 20 includes a signal light collector 21 and a photon counting module 22, the signal light collector 21 and the photon counting module 22 are sequentially disposed along the emission direction of the signal light, the signal light collector 21 is disposed in the pre-chamber 11, and the photon counting module 22 is disposed in the detection chamber 12. When the optical signal in the reaction container is tested, the reaction container is placed in the reading chamber, the reactant in the reaction container is subjected to chemical reaction to send out the optical signal to the periphery, wherein a luminous surface is formed on one surface close to the signal light collector 21, the luminous surface is imaged on the photosensitive surface of the photon counting module 22 through the signal light collector 21, the imaging surface just covers the photosensitive surface, or the imaging surface is slightly smaller than the photosensitive surface, and stray light except for a non-luminous surface cannot enter the photon counting module 22, so that interference light or background light interference is reduced. It will be appreciated that the position of the signal light collector 21 in the pre-chamber 11 can be adjusted, i.e. the distance of the signal light collector 21 relative to the photon counting module 22 can be adjusted, so that the imaging surface covers the photosurface better and noise interference is minimized.
In one embodiment, the signal light collector 21 is a single convex lens, and the convex lens has the function of collecting light, so that the light image of the light emitting surface can be collected and projected on the light sensing surface of the photon counting module 22, and the focal length of the convex lens is fixed, so that the image can be easily adjusted. In addition, the single convex lens can further simplify the structure of the device and save the cost. In some embodiments, the signal light collector 21 may be a lens group composed of a plurality of convex lenses, a lens group composed of convex lenses and concave lenses, or an optical fiber, etc., so as to obtain better effect of collecting light and imaging on the photosurface of the photon counting module 22.
In one embodiment, photon counting module 22 includes a detector 23, and detector 23 may be an end window photomultiplier (Photomultiplier tube, abbreviated PMT) or similar device capable of converting weak light signals into electrical signals, which has high sensitivity and fast response, and can obtain measurements quickly. Further, photon counting module 22 may also include photon counting plate 24. The detector 23 is mounted on the photon counting plate 24 through a mounting seat, and the photon counting plate 24 is also provided with a voltage dividing circuit, a high-voltage module, a high-gain signal amplifier, a discriminator, a prescaler, a data processor and the like. It will be appreciated by those skilled in the art that in other embodiments, depending on the functional partitioning of the circuit board, the photon counting plate 24 may be more than one, such as two or more, and the multiple blocks may be directly connected by wires. The output from the prescaler is coupled to a data processor for photon pulse counting, and in some embodiments the prescaler also has a pulse shaping effect, making the pulse signal more ideal. An optical signal, such as an emitted optical signal generated from the reference optical system 30, the reaction liquid in the reaction vessel, may be irradiated onto the detector 23.
As shown in fig. 2, the reference optical system 30 includes a diffuse reflection cavity 31, a reference light source 32 and a photodetector 33 disposed in the diffuse reflection cavity 31, a photoelectric control board 34 electrically connecting the reference light source 32 and the photodetector 33, and a diaphragm 35 communicating the signal optical system 20 and the diffuse reflection cavity 31, wherein the photoelectric control board 34 is electrically connected with the photon counting module 22, and is mainly used for controlling and monitoring the luminous intensity of the reference light source 32. The diffuse reflection cavity 31, the reference light source 32, the photoelectric detector 33 and the photoelectric control panel 34 are all arranged in the reference chamber 13, the reference chamber 13 is communicated with the signal optical system 20 through the diaphragm 35, and light rays of the reference light source 32 enter the signal optical system 20 through the diaphragm 35 after being diffusely reflected in the diffuse reflection cavity 31 and are projected onto a light sensing surface of the photon counting module 22, and are detected by the photon counting module 22.
In one embodiment, the diffuse reflection cavity 31 is a cylindrical cavity, adjacent inner side walls in the cylindrical cavity are perpendicular to each other, so that the diffusely reflected light is refracted and reflected for multiple times, the optical path is increased, and diffuse reflected light with multiple different optical paths is obtained, so that the emergent light from the diffuse reflection cavity 31 is more uniform and random, and the tolerance of the optical system to manufacturing variation is improved.
In one embodiment, the reference light source 32 and the photodetector 33 are mounted on the same side of the diffuse reflection cavity 31, and the mounting height of the photodetector 33 on the photoelectric control board 34 is lower than that of the reference light source 32, so that the direct light of the reference light source 32 can be reduced to the greatest extent and the tolerance of the photodetector 33 to the difference of the mounting and positioning of the reference light source 32 can be effectively improved.
In one embodiment, the diaphragm 35 is located on the opposite side of the reference light source 32, so that a longer optical path is obtained, and the intensity of the light incident on the diaphragm 35 is further reduced.
In one embodiment, in order to further attenuate the reference light entering the diaphragm 35, a light blocking device 36 is provided in the diffuse reflecting cavity 31. For example, a light blocking device 36 is arranged on one side of the diffuse reflection cavity 31, which is close to the diaphragm 35, a light passing channel 37 is arranged above the light blocking device 36, and part of light rays of the reference light source 32 enter the diaphragm 35 through the light passing channel 37 after being diffusely reflected and then are projected to the photon counting module 22. In one embodiment, the light blocking device 36 is a riser for blocking the direct light of the reference light source 32 from entering the diaphragm 35, so as to ensure that the light passing through the diaphragm 35 is diffuse reflected, and prevent the photon counting module 22 from being damaged due to the excessive light signal projected to the photon counting module 22 through the diaphragm 35. Those skilled in the art will appreciate that the light blocking device 36 may have other configurations as long as it blocks the direct light from the reference light source 32 from entering the aperture 36. The light-passing channel 37 may be formed between the light-blocking device 36 and the diffuse reflection cavity 31, for example, the light-passing channel 37 is formed between the side surface of the light-blocking device 36 and the cavity wall of the diffuse reflection cavity 31; the light blocking device 36 may also be provided, for example, a light passing channel 37 is formed on the light blocking device 36, and the light passing channel 37 may be a light passing slit or aperture.
In one embodiment, the inner wall of the diffuse reflection cavity 31 is coated with a diffuse reflection coating or is provided with a matte glass, so as to obtain a better diffuse reflection effect.
The spectrum of the reference light emitted by the reference light source 32 substantially matches the spectral characteristics of the signal light detected by the photon counting module 22, thus minimizing the likelihood of detection differences due to the varying spectral responses of the various PMTs.
In one embodiment, the reference light source 32 is green light, such as when the signal light is a luminescent signal generated by a chemical reaction of a spiral adamantane and its derivatives in the reaction vessel. Reference light source 32 may also be blue light, such as when the signal light is a luminescent signal generated by a chemical reaction of an acridinium ester within a reaction vessel. Preferably, the reference light source 32 is an LED lamp or an OLED lamp, which is easy to control and stable in light emission, and can be used as a standard light source, and the reference light source is surface-emitting, so that the light is relatively uniform, the diffuse reflection effect is good, and the phenomenon that most of light is reflected in the same direction due to too concentrated light is avoided, so that the original purpose of diffuse reflection is overcome.
In one embodiment, in order to make the structure simpler and more compact, the diaphragm 35 is a circular small hole arranged on the side wall of the detection chamber 12, the diaphragm 35 communicates the detection chamber 12 with the reference chamber 13, and the light of the reference light source 32 enters the signal optical system 20 through the diaphragm 35 after being diffusely reflected in the diffuse reflection cavity 31 and is projected to the photon counting module 22.
In one embodiment, the reference light may also pass through the signal light collector 21 and then be projected onto the detector 23, where the diaphragm 35 may be a circular opening on the sidewall of the pre-chamber 11, and may also correct the influence of the signal light collector 21 on signal light collection, where the diaphragm 35 communicates the pre-chamber with the reference chamber, and the light of the reference light source 32 enters the signal optical system 20 through the diaphragm 35 after being diffusely reflected in the diffuse reflection cavity 31 and is projected onto the photon counting module 22.
In one embodiment, the aperture 35 is a small circular hole, and the diameter of the circular hole is 1-10 mm, for example, but not limited to, 1mm, 2mm, 3.5mm, 4mm, 6mm, 6.5mm, 7mm, 7.5mm, 8mm, 8.5mm, 9mm, 9.5mm or 10mm, etc., so that the light signal transmitted by the reference light source 32 through the aperture 35 can be accurately controlled, and better attenuated reference light is obtained and projected to the photon counting module 22. In other embodiments, the diaphragm 35 may be any entity that acts to limit the light beam in the optical system, such as a hard opaque material with light holes. The arrangement of the diaphragm 35 ensures that eventually only a small portion of the emitted light of the reference light source 32 is projected onto the photon counting module 22, avoiding exceeding the measuring range of the photon counting module 22 and even causing device damage. In addition, the reference light entering and exiting the diaphragm 35 is random diffuse reflection light, so that the emitted light of the reference light source 32 is prevented from directly entering the detector of the photon counting module 22, and inaccuracy caused by the change of the installation position of the reference light source 32 on the calibration result can be effectively reduced.
The diffuse reflection cavity 31 and the diaphragm 35 change and attenuate the emitted light of the reference light source 32, so that various optical devices such as a diffusion plate, an attenuation sheet, a reflecting mirror and the like in the prior art are avoided, the structure is simplified, the cost is saved, and the tolerance of the self-calibration dim light detection device 100 to variation in the production and manufacturing process can be improved, namely, the assembly precision requirement of the dim light detection device 100 to the reference light source 32 is not high.
In one embodiment, the photodetector 33 is a Photodiode (PD) or avalanche Photodiode (APD, AVALANCHE PHOTODIODE), or other similar device capable of converting an optical signal into an electrical signal. The photodetector 33 is used to monitor and feedback adjust the change in the luminous intensity of the reference light source 32.
The reference optical system 30 is used for calibrating the signal optical system 20, the output light intensity of the reference light source 32 is fed back and controlled and regulated by the photoelectric control board 34, after the light emitted by the reference light source 32 is diffusely reflected in the diffuse reflection cavity 31, part of the light is detected by the photoelectric detector 33, and a small part of the light is projected to the photon counting module 22 through the diaphragm 35. According to the structural characteristics of the diaphragm 35 and the diffuse reflection cavity 31, the light signal of the reference light source 32 with specific light intensity passing through the diaphragm 35 can be quantified, the light signal passing through the diaphragm 35 is not interfered by external factors, the light signal received by the photodetector 33 is in direct proportion to the light signal received by the photon counting module 22 passing through the diaphragm 35, and thus, the light signal received by the photon counting module 22 by the reference light source 32 can be measured indirectly and accurately through the photodetector 33 at the same time.
For example: let the ratio of the light signal received by the photodetector 33 to the light signal received by the photon counting module 22 through the aperture 35 be S0, assuming that the reference light source of X-ray intensity is not shifted. When the photon counting module 22 shifts, the photoelectric control board 34 controls the output light intensity of the reference light source 32 to be X light intensity, the ratio of the light signal received by the photoelectric detector 33 to the light signal received by the photon counting module 22 passing through the diaphragm 35 is measured to be S1, and the ratio is compared with S0 before shifting to obtain the calibration factor F.
After the calibration factor F is obtained, the reactant in the reaction vessel is actually measured, the reaction vessel filled with the reactant is placed in a reading chamber, the optical signal Z of the reactant is measured, the photon counting module 22 is compensated according to the calibration factor F, namely, the optical signal Z is calibrated, the accurate optical signal of the reactant in the reaction vessel is obtained, and the self-calibration effect is achieved.
It should be noted that, during calibration and detection, the box 10 is communicated with the reading chamber to realize sealing, so that external light cannot penetrate into the box 10, and interference of external light is avoided.
The self-calibrated dim light detection device 100 of the present invention, in which the signal optical system 20 is combined with the reference optical system 30, reduces the probability of inaccurate measurement data caused by the photon counting module 22 being affected by various factors. The reference optical system 30 adopts an integrating sphere-like structure, and the output light is diffuse reflection light instead of direct light, so that the stability of the reference light source can be well improved, complex optical devices are not needed, the calibration precision is improved, the complex structure is reduced, the stability is improved, the structure is simple, the weight is small, the production and manufacturing requirements are low, and the cost of the device is greatly saved on the basis of ensuring the stability and the performance of the device.
The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The above examples illustrate only one embodiment of the invention, which is described in more detail and is not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (10)
1. A self-calibrating dim light detection device, comprising:
The box body comprises a reference chamber, a prepositive chamber and a detection chamber communicated with the prepositive chamber;
The signal optical system comprises a signal light collector and a photon counting module, wherein the signal light collector and the photon counting module are sequentially arranged along the emitting direction of the signal light, the signal light collector is arranged in the front chamber, and the photon counting module is arranged in the detection chamber; and
The reference optical system is used for calibrating the signal optical system and comprises a diffuse reflection cavity, a reference light source and a photoelectric detector which are arranged in the diffuse reflection cavity, a photoelectric control board which is electrically connected with the reference light source and the photoelectric detector, and a diaphragm which is communicated with the signal optical system and the diffuse reflection cavity, wherein the photoelectric control board is electrically connected with the photon counting module; the diffuse reflection cavity, the reference light source, the photoelectric detector and the photoelectric control panel are arranged in the reference chamber, the diaphragm is communicated with the signal optical system and the reference chamber, and light of the reference light source enters the signal optical system through the diaphragm after being diffusely reflected in the diffuse reflection cavity and is projected to the photon counting module;
A light blocking device and a light passing channel are arranged in the diffuse reflection cavity, and light rays of the reference light source enter the diaphragm through the light passing channel; after the light rays emitted by the reference light source are subjected to diffuse reflection in the diffuse reflection cavity, part of the light rays are detected by the photoelectric detector;
The signal light collector is a convex lens, or a lens group formed by the convex lens and the concave lens, or an optical fiber.
2. The self-calibrating dim light detection device according to claim 1, wherein the ratio of the light signal received by the photodetector to the light signal received by the photon counting module transmitted through the diaphragm before the non-shift is S0, with the reference light source of X-ray intensity as a reference; when the drift occurs, the ratio of the optical signal received by the photoelectric detector to the optical signal received by the photon counting module passing through the diaphragm is measured to be S1, and the S1 is compared with S0 to obtain a calibration factor F for compensating the photon counting module.
3. The self-calibrating dim light detection device according to claim 1, wherein the diameter of the diaphragm is 1-10 mm.
4. A self-calibrating weak light detection device according to any one of claims 1 to 3, wherein the diffuse reflecting cavity is a cylindrical cavity.
5. The self-calibrating, low light detection device of claim 4, wherein the reference light source and photodetector are mounted on the same side of the diffuse reflective cavity, and the aperture is positioned on the opposite side of the reference light source.
6. The self-calibrating dim light detection device according to claim 1, wherein the reference light source is an LED lamp or an OLED lamp.
7. The self-calibrating, low light detection device of claim 1, wherein the photodetector is a photodiode or an avalanche photodiode.
8. The self-calibrating dim light detection device according to claim 1, wherein when the signal light collector is a convex lens, the number of convex lenses is a single.
9. The self-calibrating, low light detection device of claim 1, wherein the photon counting module comprises a photon counting plate and a detector mounted to the photon counting plate.
10. The self-calibrating, low light detection apparatus according to claim 9, wherein the detector is a photomultiplier tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810569761.7A CN108444917B (en) | 2018-06-05 | 2018-06-05 | Self-calibrating dim light detection device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810569761.7A CN108444917B (en) | 2018-06-05 | 2018-06-05 | Self-calibrating dim light detection device |
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| CN108444917A CN108444917A (en) | 2018-08-24 |
| CN108444917B true CN108444917B (en) | 2024-07-26 |
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| CN109668865B (en) * | 2019-01-04 | 2024-08-16 | 上海科源电子科技有限公司 | Fluorescence detector stability measuring device and measuring method |
| CN109916514A (en) * | 2019-03-19 | 2019-06-21 | 谱诉光电科技（苏州）有限公司 | A kind of photon injection type Dim light measurement method and device |
| CN110879208A (en) * | 2019-12-04 | 2020-03-13 | 北京贝泰科技有限公司 | Take absorbance detection system of self calibration function |
| WO2021119954A1 (en) * | 2019-12-16 | 2021-06-24 | 谱诉光电科技（苏州）有限公司 | Weak light measurement method |
| CN111060199B (en) * | 2019-12-26 | 2022-07-12 | 迪瑞医疗科技股份有限公司 | Optical detection system with self-checking device and self-checking method |
| CN112362620A (en) * | 2020-10-16 | 2021-02-12 | 石家庄禾柏生物技术股份有限公司 | Multi-methodology combined optical path |
| CN112903096A (en) * | 2021-03-25 | 2021-06-04 | 安图实验仪器(郑州)有限公司 | Standard light source device |
| CN114812801A (en) * | 2022-04-27 | 2022-07-29 | 三诺生物传感股份有限公司 | Calibration method and calibration device for photon counting device |
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