CN108432810B - Botanical insecticidal composition, preparation and application thereof in preventing and controlling crop pests - Google Patents
Botanical insecticidal composition, preparation and application thereof in preventing and controlling crop pests Download PDFInfo
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- CN108432810B CN108432810B CN201810322064.1A CN201810322064A CN108432810B CN 108432810 B CN108432810 B CN 108432810B CN 201810322064 A CN201810322064 A CN 201810322064A CN 108432810 B CN108432810 B CN 108432810B
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- 239000000203 mixture Substances 0.000 title claims abstract description 67
- 238000002360 preparation method Methods 0.000 title claims abstract description 33
- 230000000749 insecticidal effect Effects 0.000 title claims abstract description 28
- 241000607479 Yersinia pestis Species 0.000 title claims abstract description 19
- NAHTXVIXCMUDLF-DUJPAFNYSA-N Toosendanin Natural products O=C(O[C@H]1C(=O)[C@H]2[C@@](C)([C@H](O)C[C@H]3[C@@]4(C)[C@H](O)OC[C@@]23[C@@H](O)C[C@H]4OC(=O)C)[C@@]23[C@]1(C)[C@H](c1cocc1)C[C@H]2O3)C NAHTXVIXCMUDLF-DUJPAFNYSA-N 0.000 claims abstract description 39
- NAHTXVIXCMUDLF-RFNFAWMESA-N toosendanin Chemical compound C=1([C@H]2[C@]3(C)[C@@H](OC(C)=O)C(=O)[C@H]4[C@@]([C@@]53O[C@@H]5C2)(C)[C@H](O)C[C@H]2[C@@]3(C)[C@H](O)OC[C@]24[C@@H](O)C[C@H]3OC(=O)C)C=COC=1 NAHTXVIXCMUDLF-RFNFAWMESA-N 0.000 claims abstract description 39
- 239000008488 toosendanin Substances 0.000 claims abstract description 39
- 241000212948 Cnidium Species 0.000 claims abstract description 38
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 36
- 239000000575 pesticide Substances 0.000 claims abstract description 20
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- 239000007788 liquid Substances 0.000 claims abstract description 10
- 239000004480 active ingredient Substances 0.000 claims abstract description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 19
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- 244000241257 Cucumis melo Species 0.000 claims 1
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- 230000002195 synergetic effect Effects 0.000 abstract description 18
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- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 15
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- 241000219112 Cucumis Species 0.000 description 4
- 239000001293 FEMA 3089 Substances 0.000 description 4
- 241000510199 Helicoverpa assulta Species 0.000 description 4
- 240000000233 Melia azedarach Species 0.000 description 4
- HPUXDMUGCAWDFW-UHFFFAOYSA-N Osthole Natural products COc1ccc2CCC(=O)Oc2c1C=CC(=O)C HPUXDMUGCAWDFW-UHFFFAOYSA-N 0.000 description 4
- 125000005910 alkyl carbonate group Chemical group 0.000 description 4
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- 239000004359 castor oil Substances 0.000 description 4
- 235000019438 castor oil Nutrition 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 4
- 229930182470 glycoside Natural products 0.000 description 4
- 229960003639 laurocapram Drugs 0.000 description 4
- MBRLOUHOWLUMFF-UHFFFAOYSA-N osthole Chemical compound C1=CC(=O)OC2=C(CC=C(C)C)C(OC)=CC=C21 MBRLOUHOWLUMFF-UHFFFAOYSA-N 0.000 description 4
- 230000000607 poisoning effect Effects 0.000 description 4
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- DMAXMXPDVWTIRV-UHFFFAOYSA-N 2-(2-phenylethyl)phenol Chemical compound OC1=CC=CC=C1CCC1=CC=CC=C1 DMAXMXPDVWTIRV-UHFFFAOYSA-N 0.000 description 3
- 235000009024 Ceanothus sanguineus Nutrition 0.000 description 3
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- MJPMPMZXJIZPRE-UHFFFAOYSA-N 3-butyl-5,6,7,7a-tetrahydro-3h-2-benzofuran-1-one Chemical compound C1CCC=C2C(CCCC)OC(=O)C21 MJPMPMZXJIZPRE-UHFFFAOYSA-N 0.000 description 2
- 241000208173 Apiaceae Species 0.000 description 2
- 241000244186 Ascaris Species 0.000 description 2
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- 241000406668 Loxodonta cyclotis Species 0.000 description 2
- 241000158723 Melia Species 0.000 description 2
- 208000005374 Poisoning Diseases 0.000 description 2
- 244000296102 Selinum monnieri Species 0.000 description 2
- 235000019084 Selinum monnieri Nutrition 0.000 description 2
- 241000753145 Sitotroga cerealella Species 0.000 description 2
- 235000003161 Solanum erianthum Nutrition 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
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- 239000002778 food additive Substances 0.000 description 2
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- 244000144972 livestock Species 0.000 description 2
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- 235000000832 Ayote Nutrition 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000009849 Cucumis sativus Nutrition 0.000 description 1
- 240000004244 Cucurbita moschata Species 0.000 description 1
- 235000009854 Cucurbita moschata Nutrition 0.000 description 1
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 1
- 241001147381 Helicoverpa armigera Species 0.000 description 1
- 235000003956 Luffa Nutrition 0.000 description 1
- 244000050983 Luffa operculata Species 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 244000070406 Malus silvestris Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 241000220324 Pyrus Species 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 241000830536 Tripterygium wilfordii Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000002528 anti-freeze Effects 0.000 description 1
- 235000021016 apples Nutrition 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000021332 kidney beans Nutrition 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 231100000989 no adverse effect Toxicity 0.000 description 1
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- 229920002503 polyoxyethylene-polyoxypropylene Polymers 0.000 description 1
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- 231100000820 toxicity test Toxicity 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/10—Apiaceae or Umbelliferae [Carrot family], e.g. parsley, caraway, dill, lovage, fennel or snakebed
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Pest Control & Pesticides (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a botanical insecticidal composition, a preparation and application thereof in preventing and treating crop pests, wherein the composition at least contains active ingredients of toosendanin and cnidium fruit oil, the weight percentage of the toosendanin to the cnidium fruit oil is 3-5: 4-10, and when the composition is prepared into a preparation, the preparation form can be environment-friendly missible oil, microemulsion or soluble liquid. The composition has higher control effect on various pests in agricultural production, does not contain chemical pesticide components, and the pests are not easy to generate drug resistance; the composition has a synergistic effect, reduces the using amount of pesticides, is easy to degrade in the environment, and is suitable for preventing and controlling various organic crop pests.
Description
Technical Field
The invention belongs to the technical field of pesticides, and relates to a botanical insecticidal composition, a preparation and application thereof in preventing and controlling crop pests.
Background
Toosendanin is an effective insecticidal component extracted from root bark of Melia toosendan or Melia azedarach belonging to Melia of traditional Chinese medicine for expelling ascaris. The toosendan fruit preparation has good antifeedant, systemic poisoning and stomach poisoning effects and certain growth inhibition effect, is safe, has good prevention and treatment effects and is environment-friendly.
The cnidium fruit oil is extracted from dried cnidium fruit of cnidium monnieri (L.) DC of Umbelliferae, and can be used as medicine, health product, beverage, food additive, etc. containing osthole. The botanical pesticide with osthole as active component is used in preventing and controlling stored grain moth, booklice and corn elephant and has high activity on cabbage caterpillar.
The single agent is used for preventing and controlling pests, and the drug resistance is easy to generate. In agricultural production, through reasonable compatibility of different components, the method is a common method for preventing and controlling the drug resistance of pests according to the compounding effect. A botanical synergistic compound pesticide containing toosendanin and a preparation method thereof (CN 102239891) disclose a pesticide prepared by mixing toosendanin and tripterygium wilfordii alkaloid. Although toosendanin and cnidium lactone are common botanical insecticides, no related public report exists on a pesticide composition which has a good synergistic effect due to the mutual compatibility and synergistic effect of toosendanin and cnidium seed oil.
Disclosure of Invention
The first purpose of the invention is to provide a botanical insecticidal composition, which at least comprises toosendanin and cnidium fruit oil, and the composition has a synergistic effect, low use cost and good control effect.
The second purpose of the invention is to provide a plant source insecticidal composition preparation, and the preparation of the insecticidal composition preparation can be prepared by a common processing method known by a person skilled in the art, and a corresponding auxiliary agent such as a surfactant is added to prepare a preparation product, preferably an environment-friendly preparation formulation.
The botanical insecticidal composition or the insecticidal composition preparation of the invention is applied to the preparation of the pesticide for preventing and controlling the crop pests.
In order to achieve the purpose, the technical scheme adopted by the invention comprises the following steps:
a botanical insecticidal composition at least comprises toosendanin and cnidium fruit oil, wherein the weight ratio of the toosendanin to the cnidium fruit oil in the composition is 3-5: 4-10.
A botanical insecticidal composition formulation comprising an insecticidally active ingredient and at least one adjuvant;
the insecticidal active ingredient is the plant source insecticidal composition of claim 1;
the auxiliary agent can be one or a mixture of more than one of an emulsifier, a synergist, a surfactant, an antifreezing agent and a solvent.
Optionally, the mass percentage of the toosendanin in the preparation is 0.3-0.5% of the total weight, and the mass percentage of the cnidium fruit oil in the preparation is 0.4-1% of the total weight.
Optionally, the formulation may be an emulsifiable concentrate, a microemulsion, or a soluble liquid.
Optionally, the missible oil comprises the following components in percentage by mass: 3 to 5 percent of toosendanin, 4 to 10 percent of cnidium fruit oil, 5 to 10 percent of emulsifier, 5 to 20 percent of synergist and 100 percent of organic solvent.
Optionally, the microemulsion comprises, by mass percent: 3-5% of toosendanin, 4-10% of cnidium fruit oil, 10-20% of organic solvent, 5-15% of surfactant, 0-20% of synergist, 3-6% of antifreezing agent and 100% of water.
Optionally, the soluble agent comprises, by mass percent: 3-5% of toosendanin, 4-10% of cnidium fruit oil, 5-20% of surfactant, 0-20% of synergist, 4-10% of organic solvent and 100% of water.
The plant-derived insecticidal composition or the plant-derived insecticidal composition preparation is applied to preparation of pesticides for controlling pests on crops.
Optionally, the crop may be melons, fruits, vegetables, tobacco and/or tea plants.
Optionally, the pests can be oriental tobacco budworms, cabbage caterpillars, tea caterpillars, diamond back moths, tea geometrids, prodenia litura, cotton bollworms, beet armyworms and/or armyworms.
The invention has the advantages that:
the invention adopts environment-friendly missible oil, microemulsion and soluble liquid agent which are processed by adding auxiliary agents after the toosendanin and the cnidium fruit oil are reasonably compounded, and the environment-friendly missible oil, microemulsion and soluble liquid agent are used for preventing and controlling cabbage caterpillar, oriental tobacco caterpillar, tea caterpillar, diamond back moth, tea geometrid, prodenia litura, cotton bollworm, beet armyworm, armyworm and the like on melon, fruit, vegetable, tobacco and tea trees, and have good prevention and control effects.
The natural active ingredients extracted from the plant are easily degradable in the environment, safe to people and livestock and safe to natural enemies, and the pests are not easy to generate drug resistance; the preparation formulation of the invention is environment-friendly emulsifiable oil, microemulsion and soluble solution, and has safe and environment-friendly preparation formulation and simple operation. The composition has synergistic effect, reduces the dosage of pesticide, and reduces environmental pollution.
Detailed Description
The weight ratio of the insecticidal composition containing toosendanin and cnidium seed oil provided by the invention is 3-5: 4-10;
in the botanical insecticidal composition preparation, the weight percentage of the active component of the toosendanin accounts for 0.3-0.5% of the total weight, and the weight percentage of the cnidium fruit oil accounts for 0.4-1% of the total weight, so that environment-friendly emulsifiable oil, microemulsion and soluble liquid can be prepared.
Toosendanin is an effective insecticidal component extracted from root bark of Melia toosendan or Melia azedarach belonging to Melia of traditional Chinese medicine for expelling ascaris. The toosendan fruit preparation has good antifeedant, systemic poisoning and stomach poisoning effects and certain growth inhibition effect, is safe, has good prevention and treatment effects and is environment-friendly.
The cnidium fruit oil is extracted from dried cnidium fruit of cnidium monnieri (L.) DC of Umbelliferae, and can be used as medicine, health product, beverage, food additive, etc. containing osthole. The botanical pesticide with osthole as active component is used in preventing and controlling stored grain moth, booklice and corn elephant and has high activity on cabbage caterpillar.
The insecticidal composition of the invention can be prepared by common processing methods known to those skilled in the art, and added with corresponding auxiliary agents such as surfactants to prepare preparation products. The auxiliary agent can be one or a mixture of more than one of an emulsifier, a synergist, a surfactant, an antifreezing agent and a solvent;
such as: the emulsifier is one or more of alkyl benzene sulfonate, alkylphenol polyoxyethylene ether, phenethyl phenol polyoxypropylene ether and similar substances thereof, and tween. The synergist is one or more of organosilicon, synergistic ether, synergistic amine, alkyl carbonate and laurocapram. The surfactant is one or more of alkyl glycoside, sucrose glyceride, lignosulfonate, polyoxyethylene octylphenol ether, castor oil and aralkyl phenol polyoxyethylene ether formaldehyde condensate. The antifreezing agent is ethylene glycol or glycerol. The organic solvent is one or more of water, ethanol, isopropanol, acetone, ethyl acetate, turpentine oil and diformate.
The missible oil prepared from the composition comprises the following components in percentage by mass: 3 to 5 percent of toosendanin, 4 to 10 percent of cnidium fruit oil, 5 to 10 percent of emulsifier, 5 to 20 percent of synergist and 100 percent of organic solvent.
The microemulsion prepared from the composition comprises the following components in percentage by mass: 3-5% of toosendanin, 4-10% of cnidium fruit oil, 10-20% of organic solvent, 5-15% of surfactant, 0-20% of synergist, 3-6% of antifreezing agent and 100% of water.
The composition is prepared into soluble solution by the following components in percentage by mass: 3-5% of toosendanin, 4-10% of cnidium fruit oil, 5-20% of surfactant, 0-20% of synergist, 4-10% of organic solvent and 100% of water.
Experiments in different places all over the country show that the control effects of the toosendanin and the cnidium seed oil after being mixed are superior to the control effects of single agents on the control effects of green tobacco budworms, cabbage caterpillars, tea caterpillars, diamond back moths, tea geometrids, prodenia litura, cotton bollworms, beet armyworms and armyworms on melons (cucumbers, pumpkin, luffa and the like), fruits (apples, juicy peaches, pears, oranges and the like), vegetables (cabbages, tomatoes, kidney beans and the like), tobaccos and tea trees.
The research method comprises the following steps:
the invention adopts a method combining indoor biological assay and field test. Firstly, determining the toxicity of each single agent, the drug effects of the two agents in different proportions and the co-toxicity coefficient (CTC) after different proportions are compounded by indoor toxicity determination, wherein the CTC is less than 80 and is antagonistic, the CTC is more than or equal to 80 and is less than or equal to 120 and is additive, and the CTC has a synergistic effect more than 120, and then carrying out field test.
The test method comprises the following steps: during the test, the mother liquor of each mixture is respectively diluted into five series of concentrations and respectively placed in beakers for standby. Soaking the test insects together with the leaves in the medicinal liquid for 5s, taking out, naturally drying, placing into an insect box, feeding at 25 deg.C, repeating for 3 times each time, wherein the number of test insects used in each repetition is 10, and using clear water as reference,checking the number of dead insects at 48h, calculating the mortality rate and correcting the mortality rate, solving a toxicity regression equation and calculating LC50The value is obtained. And (4) calculating a co-toxicity coefficient CTC according to a Sun cloud Peel formula method.
Example 1:
the tested pests: oriental tobacco budworm;
the test agents are provided by Daphne foenum-graecum Biotech limited;
virulence assay results are shown in table 1:
TABLE 1 toxicity test results of the combination of toosendanin and cnidium seed oil on Nicotiana tabacum
As can be seen from Table 1, the mixture ratio of the toosendanin and the cnidium fruit oil is 3% -5% and 4% -10%, the cotoxicity coefficient CTC is more than 120, and the composition has a synergistic effect on oriental tobacco budworms.
The above-described respective formulations were configured as follows:
1. environment-friendly Emulsifiable Concentrate (EC): according to the mass percentage, the toosendanin accounts for 3-5%, the cnidium fruit oil accounts for 4-10%, the emulsifier accounts for 5-10%, the synergist accounts for 5-20%, and the organic solvent is complemented to 100%. The raw materials are weighed according to the formula, added and mixed in sequence, and mixed evenly in a reaction kettle to prepare the missible oil preparation.
The emulsifier is one or more of alkyl benzene sulfonate, alkylphenol polyoxyethylene ether, phenethyl phenol polyoxypropylene ether and similar substances thereof, and tween;
the synergist is one or more of organosilicon, synergistic ether, synergistic amine, alkyl carbonate and laurocapram;
the organic solvent is one or more of water, ethanol, isopropanol, acetone, ethyl acetate, turpentine oil and diformate.
2. Microemulsion (ME), by mass percentage, 3-5% of toosendanin, 4-10% of cnidium fruit oil, 10-20% of organic solvent, 5-15% of surfactant, 0-20% of synergist, 3-6% of antifreeze and 100% of water. Weighing raw materials, sequentially adding the raw materials, mixing, and stirring in a reaction kettle until the mixture is transparent to obtain the microemulsion.
The organic solvent is one or a mixture of more than one of water, ethanol, isopropanol, acetone, ethyl acetate, turpentine oil and diformate;
the surfactant is one or a mixture of more than one of alkyl glycoside, sucrose glyceride, lignosulfonate, octylphenol polyoxyethylene ether, castor oil and aralkyl phenol polyoxyethylene ether formaldehyde condensate;
the synergist is one or more of organosilicon, synergistic ether, synergistic amine, alkyl carbonate and laurocapram;
the antifreezing agent is ethylene glycol or glycerol.
3. Solubles (SL): according to the mass percentage, the toosendanin accounts for 3-5%, the cnidium fruit oil accounts for 4-10%, the surfactant accounts for 5-20%, the synergist accounts for 0-20%, the organic solvent accounts for 4-10%, and the water is supplemented to 100%. The raw materials are weighed according to the formula, are sequentially added and mixed, and are uniformly stirred in a reaction kettle to prepare the soluble agent.
The surfactant is one or a mixture of more than one of alkyl glycoside, sucrose glyceride, lignosulfonate, octylphenol polyoxyethylene ether, castor oil and aralkyl phenol polyoxyethylene ether formaldehyde condensate;
the synergist is one or more of organosilicon, synergistic ether, synergistic amine, alkyl carbonate and laurocapram;
the organic solvent is one or more of water, ethanol, isopropanol, acetone, ethyl acetate, turpentine oil and diformate.
Example 2:
preparing a mixed medicament:
1.2 percent of toosendanin-cnidium fruit oil environment-friendly missible oil is prepared:
taking 12 parts of toosendanin-cnidium seed oil (the compound ratio of toosendanin to cnidium seed oil is 5:7), adding 12 parts of organic silicon, 4 parts of synergistic ether, 3 parts of phenethyl phenol polyoxyethylene polyoxypropylene ether, 5 parts of ethyl acetate and ethanol to 100 parts of the mixture, and stirring at the stirring speed of 800-1000 revolutions per minute for 10-30 minutes to obtain the 1.2% toosendanin-cnidium seed oil environment-friendly missible oil.
Preparing 1% toosendanin-cnidium seed oil microemulsion:
taking 10 parts of toosendanin-cnidium seed oil (the compound ratio of toosendanin to cnidium seed oil is 4:6), adding 11 parts of organic silicon, 3 parts of ethylene glycol, 5 parts of op-10, 15 parts of ethanol and water to 100 parts of the mixture, and stirring the mixture in a preparation kettle at a stirring speed of 60-80 revolutions per minute for 20-40 minutes to obtain the 1% toosendanin-cnidium seed oil microemulsion.
0.7 percent of toosendanin-cnidium seed oil soluble liquid preparation:
and (2) taking 7 parts of toosendanin-cnidium seed oil (the compound ratio of the toosendanin to the cnidium seed oil is 3:4), sequentially adding 4 parts of alkyl glycoside, 4 parts of ethanol, 5 parts of castor oil, 5 parts of glycerol, 5 parts of acetone and 100 parts of water into the mixture, uniformly mixing, and stirring at the stirring speed of 100-200 revolutions per minute for 10-30 minutes to prepare the 0.7% toosendanin-cnidium seed oil soluble liquid.
Pharmacodynamic test part: 1.2 percent of toosendanin-cnidium seed oil medicament, 1 percent of toosendanin-cnidium seed oil microemulsion, 0.7 percent of toosendanin-cnidium seed oil soluble liquid agent, 0.3 percent of toosendanin missible oil and 0.4 percent of cnidium seed oil missible oil (purchased in the market) are prepared. The test is arranged in suburbs of Shandong Jinnan, the insect pest indexes of tobacco smoke are investigated before pesticide application, the pesticide application is carried out at the initial stage of insect pest occurrence, the insect pest indexes are investigated 3 days, 10 days and 20 days after the pesticide application, and the control effect is calculated. The test results are shown in table 2 below:
TABLE 2 statistics of drug effects
As can be seen from the experimental results in Table 2, the pesticide composition prepared by compounding toosendanin and cnidium seed oil has better control effect than single pesticide, has synergistic effect, has longer control period and has no adverse effect on crops under the control of pesticide. The usage amount of pesticides is reduced, the environmental pollution is avoided, and the pesticide is safe to people and livestock and environment-friendly.
Experiments in different places all over the country show that the control effects of the toosendanin and the cnidium seed oil after being mixed on melons, fruits, vegetables, tobacco and tea trees are better than the control effects of single agents on oriental tobacco budworms, cabbage caterpillars, tea caterpillars, diamond back moths, tea geometrids, prodenia litura, cotton bollworms, beet armyworms and armyworms.
Claims (10)
1. The botanical insecticidal composition is characterized by at least comprising toosendanin and cnidium fruit oil, wherein the weight ratio of the toosendanin to the cnidium fruit oil in the composition is 3-5: 4-10.
2. A botanical insecticidal composition formulation characterized in that said formulation comprises an insecticidally active ingredient and at least one adjuvant; the insecticidal active ingredient is the plant source insecticidal composition of claim 1; the auxiliary agent is one or a mixture of more than one of an emulsifier, a synergist, a surfactant, an antifreezing agent and a solvent.
3. The botanical insecticide composition preparation according to claim 2, wherein the mass percentage of toosendanin in the preparation is 3% to 5% of the total weight, and the mass percentage of cnidium fruit oil in the preparation is 4% to 10% of the total weight.
4. The formulation of botanical insecticidal composition as claimed in claim 2 or 3 wherein the formulation is an emulsifiable concentrate, a microemulsion or a liquid soluble formulation.
5. The plant-derived insecticidal composition preparation according to claim 4, wherein said emulsifiable concentrate comprises, in mass percent: 3 to 5 percent of toosendanin, 4 to 10 percent of cnidium fruit oil, 5 to 10 percent of emulsifier, 5 to 20 percent of synergist and 100 percent of organic solvent.
6. The formulation of botanical insecticidal composition as claimed in claim 4 wherein the microemulsion comprises, in mass percent: 3-5% of toosendanin, 4-10% of cnidium fruit oil, 10-20% of organic solvent, 5-15% of surfactant, 0-20% of synergist, 3-6% of antifreezing agent and 100% of water.
7. The formulation of botanical insecticidal composition as claimed in claim 4 wherein said solubles comprise, in mass percent: 3-5% of toosendanin, 4-10% of cnidium fruit oil, 5-20% of surfactant, 0-20% of synergist, 4-10% of organic solvent and 100% of water.
8. Use of the plant-derived pesticidal composition of claim 1 or the plant-derived pesticidal composition preparation of any one of claims 2 to 7 for the preparation of a pesticide for controlling pests on crops.
9. Use according to claim 8, wherein the crop plants are melons, fruits, vegetables, tobacco and/or tea plants.
10. The use of claim 8, wherein the pests are cabbage caterpillar, tea caterpillar, diamond back moth, tea geometrid, prodenia litura, cotton bollworm, beet armyworm and/or armyworm.
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