CN108192813A - A kind of trace dna elutes collection device - Google Patents
A kind of trace dna elutes collection device Download PDFInfo
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- CN108192813A CN108192813A CN201810310099.3A CN201810310099A CN108192813A CN 108192813 A CN108192813 A CN 108192813A CN 201810310099 A CN201810310099 A CN 201810310099A CN 108192813 A CN108192813 A CN 108192813A
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- nucleic acid
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- 238000001821 nucleic acid purification Methods 0.000 claims abstract description 36
- 238000010828 elution Methods 0.000 claims abstract description 21
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 18
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 18
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 16
- 230000005484 gravity Effects 0.000 claims description 5
- 238000012423 maintenance Methods 0.000 claims description 3
- 230000008719 thickening Effects 0.000 claims description 2
- 239000002253 acid Substances 0.000 claims 1
- 238000005119 centrifugation Methods 0.000 abstract description 8
- 239000003480 eluent Substances 0.000 abstract description 5
- 238000000605 extraction Methods 0.000 abstract description 5
- 238000000034 method Methods 0.000 abstract description 4
- 238000004321 preservation Methods 0.000 abstract description 2
- 238000005406 washing Methods 0.000 abstract description 2
- 108020004414 DNA Proteins 0.000 description 23
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 6
- 238000001514 detection method Methods 0.000 description 5
- 102000053602 DNA Human genes 0.000 description 4
- 230000007067 DNA methylation Effects 0.000 description 4
- 108020004682 Single-Stranded DNA Proteins 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 238000003556 assay Methods 0.000 description 3
- 229940104302 cytosine Drugs 0.000 description 3
- 230000009615 deamination Effects 0.000 description 3
- 238000006481 deamination reaction Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 210000003811 finger Anatomy 0.000 description 3
- 210000005224 forefinger Anatomy 0.000 description 3
- 210000003813 thumb Anatomy 0.000 description 3
- 206010006187 Breast cancer Diseases 0.000 description 2
- 208000026310 Breast neoplasm Diseases 0.000 description 2
- 206010009944 Colon cancer Diseases 0.000 description 2
- 208000001333 Colorectal Neoplasms Diseases 0.000 description 2
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 2
- -1 Nucleic acid small molecule Chemical class 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 238000000703 high-speed centrifugation Methods 0.000 description 2
- 201000005202 lung cancer Diseases 0.000 description 2
- 208000020816 lung neoplasm Diseases 0.000 description 2
- 239000012188 paraffin wax Substances 0.000 description 2
- 230000009946 DNA mutation Effects 0.000 description 1
- 101150039808 Egfr gene Proteins 0.000 description 1
- 206010064571 Gene mutation Diseases 0.000 description 1
- 101150105104 Kras gene Proteins 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 102000052116 epidermal growth factor receptor activity proteins Human genes 0.000 description 1
- 108700021358 erbB-1 Genes Proteins 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000012263 liquid product Substances 0.000 description 1
- 210000004932 little finger Anatomy 0.000 description 1
- 230000009401 metastasis Effects 0.000 description 1
- YOHYSYJDKVYCJI-UHFFFAOYSA-N n-[3-[[6-[3-(trifluoromethyl)anilino]pyrimidin-4-yl]amino]phenyl]cyclopropanecarboxamide Chemical compound FC(F)(F)C1=CC=CC(NC=2N=CN=C(NC=3C=C(NC(=O)C4CC4)C=CC=3)C=2)=C1 YOHYSYJDKVYCJI-UHFFFAOYSA-N 0.000 description 1
- JOHZPMXAZQZXHR-UHFFFAOYSA-N pipemidic acid Chemical compound N1=C2N(CC)C=C(C(O)=O)C(=O)C2=CN=C1N1CCNCC1 JOHZPMXAZQZXHR-UHFFFAOYSA-N 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- Engineering & Computer Science (AREA)
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- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
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- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
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- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of trace dnas to elute collection device, the present apparatus effectively solves the problems, such as nucleic acid extraction micro eluent collection and preservation in purifying with a kind of simple scheme, three components of entire washing device assemble and disassemble process simply, quickly, lightly, without firmly plug.One nucleic acid purification post and a microcentrifugation pipe are packaged in a casing, are not in obscure and entanglement when marking sample.It is neat and tidy during centrifugation, be not in that lid fractures or break up.Elution is collected part and is included in casing, and mutually closing is independent, is not in the pollution between sample in centrifugation.Collecting pipe volume matches with collecting effluent volume, is conducive to preserve for a long time.Collecting pipe is small, and tube spacing can be adapted with 8 rows or 12 row's multichannel pipettors, can carry out batch and sample sample-adding.
Description
Technical field
The present invention relates to biomedicine technical field, specifically a kind of trace dna elutes collection device.
Background technology
Nucleic acid small molecule is extracted from cell and is purified, the requirement that concentration and purity meet detection is allowed to, is into
Work(measures the premise of nucleic acid small molecule.In clinical medicine and research application, lung cancer, colorectal cancer patients are receiving targeted drug
It needs to carry out EGFR or KRAS gene mutation detection before treatment, needs to extract from lung cancer or colorectal cancer paraffin tissue sections and pure
Change DNA, DNA can be used for abrupt climatic change.The table of more than ten genes of progress is needed during the relapse and metastasis risk for assessing breast cancer
Up to quantitative analysis, RNA need to be extracted from breast cancer paraffin tissue sections and purified, RNA can be used for and quantitatively detected.Carry out
During the DNA methylation assay of DNA, generally need to 300-500ngDNA be subjected to cytosine deamination conversion, form single stranded DNA, then will be single
Chain DNA purifies, and can be used for DNA methylation assay.
In above-mentioned DNA mutation, RNA is quantitative and DNA methylation detection in, detect sample extraction nucleic acid usually compared with
It is few.To ensure nucleic acid concentration needed for detection, the solution of eluted dna is only 20-30 microlitres after extraction.Especially for DNA methylation assay
It is preceding to cytosine deamination treated single stranded DNA purifies when, elution liquor capacity is only 10 microlitres.At present, either
The nucleic acid extraction and purification kit of commercialization or laboratory Self-made reagent box, trace dna elutes after purification uses 1.5
The centrifuge tube of milliliter volume is collected.1.5 milliliters of centrifuge tubes are significantly excessive for 10-30 microlitres of elution liquor capacity.Nothing
Method is directly loaded in batches after elution with the multichannel pipettor of 8 rows or 12 rows, and when batch is loaded, the used time is longer.Large volume is collected
Pipe, often because being condensed in tube wall again after moisture evaporation or moisture evaporation, causes nucleic acid adhesive in pipe in 4 degree of refrigeration of refrigerator
Wall since nucleic acid concentration is relatively low in itself, can not be gone to wash down the nucleic acid for attaching to tube wall, purified nucleic acid with large volume liquid
It will lose, influence subsequent detection.Treated that purification of single stranded DNA is especially apparent for cytosine deamination for problems,
Treated, and single stranded DNA is easily degraded in itself, when being eluted in 1.5 milliliters of centrifuge tube with 10 microlitres of volume, often more
It is difficult to preserve.In addition, the lid that 1.5 milliliters of centrifuge tubes are usually connected with faciola, is often covered when high speed centrifugation elutes
It situations such as tear that sub- connect band is broken, lid comes off, makes troubles to elution action, is especially centrifuging elution simultaneously compared with multisample
When, centrifuge is not easy to balance, and lid fracture is especially common.
Therefore, a kind of special container or device to match with micro elution volume is needed when trace dna extracts, it should
Container or device can should conveniently complete the preservation purification of nucleic acid that eluent is collected and long-time is safe, and energy side
Continue batch after an action of the bowels to be loaded.
Invention content
The purpose of the present invention is to provide a kind of trace dnas to elute collection device, to solve to propose in above-mentioned background technology
The problem of.
To achieve the above object, the present invention provides following technical solution：
A kind of trace dna elutes collection device, including nucleic acid purification post, microcentrifugation pipe and casing.
Preferred embodiment as the present invention：Described sleeve pipe assembles up other two component so as to shape as maintenance medium
Into an entirety, casing main body is cylinder, lower end closed, upper end opening, and set tube wall is transparent.
As further preferred embodiment of the invention：The microcentrifugation pipe includes lid and microcentrifugation
Pipe pipe shaft, microcentrifugation pipe pipe shaft upper end are openning shape, and lower part is the cone of closing, and microcentrifugation pipe pipe shaft is most
Big outer diameter is less than the internal diameter of casing.
As further preferred embodiment of the invention：The lid of the microcentrifugation pipe and microcentrifugation pipe
Pipe shaft can be kept completely separate, and microcentrifugation pipe pipe shaft middle and upper part part tube wall annularly thickens.
As further preferred embodiment of the invention：The nucleic acid purification post main body is cylinder, and lower end, which is shunk, to attenuate,
Nucleic acid purification post body outer diameter is less than casing inner diameter, and casing can be fallen by gravity when upright.
As further preferred embodiment of the invention：Device various components assemble when opening up upright it is integral,
Three components can be automatically separated when flipping upside down down on the whole.
As further preferred embodiment of the invention：The nucleic acid purification post lower end and microcentrifugation pipe upper end phase
Connection, nucleic acid purification post lower end taper portion are inserted into inside microcentrifugation pipe upper end opening.
As further preferred embodiment of the invention：The microcentrifugation bottom of the tube is in contact with sleeve bottom,
Microcentrifugation pipe pipe shaft ring-type thickening is in contact with internal surface of sleeve pipe, and nucleic acid purification column outer wall connects with internal surface of sleeve pipe
It touches.
Compared with prior art, the beneficial effects of the invention are as follows：The present apparatus is effectively solved with a kind of simple scheme
The problem of micro eluent is collected and preserved in nucleic acid extraction purifying, three components of entire washing device assemble and disassemble process
Simply, quickly, lightly, without firmly plugging.One nucleic acid purification post and a microcentrifugation pipe are packaged in a casing,
Be not in obscure and entanglement when marking sample.It is neat and tidy during centrifugation, be not in that lid fractures or break up.Elute collection portion
Divide and be included in casing, mutually closing is independent, is not in the pollution between sample in centrifugation.Collecting pipe volume is eluted with collecting
Liquid product matches, and is conducive to preserve for a long time.Collecting pipe is small, and tube spacing can mutually be fitted with 8 rows or 12 row's multichannel pipettors
Match, batch can be carried out and sample sample-adding.
Description of the drawings
Fig. 1 is the structure diagram of the first embodiment of the invention.
Fig. 2 is the structure diagram of second of embodiment of the invention.
Fig. 3 is the structure diagram of the third embodiment of the invention.
Specific embodiment
Below in conjunction with the attached drawing in the embodiment of the present invention, the technical solution in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other without making creative work
Embodiment shall fall within the protection scope of the present invention.
Referring to Fig. 1, in the first embodiment of the invention, a kind of trace dna elutes collection device, including pure by nucleic acid
Change the entirety that column 001, microcentrifugation pipe pipe shaft 003 and casing 004 are assembled into one, casing 004 is situated between as maintenance
Matter fits together nucleic acid purification post 001 and microcentrifugation pipe pipe shaft 004, and 004 tube wall of casing is transparent, can be seen that casing
Internal objects.Casing 004 outer diameter 12mm, internal diameter 10mm.003 top of microcentrifugation pipe pipe shaft is cylindrical, outer diameter 6mm,
Lower part is conical, bottom lock.Microcentrifugation pipe includes lid 002 and pipe shaft 003, and the two can be kept completely separate.It is micro
The region that 3mm long below pipe upper end opening is collected by centrifugation is screw thread 006.After the completion of elution, lid 002 and pipe shaft 003 pass through
Screw thread screws, and makes the microcentrifugation seal of tube.The region of the 006 lower section 1mm of screw thread of pipe shaft 003 is thickened for the ring-type of tube wall
Band 007, outer diameter is 8mm herein.When assembling trace dna elution collection device 005, microcentrifugation pipe pipe shaft 003 is put into
Casing 004, pipe shaft 003 drop to 004 bottom end of casing under the action of can relying on gravity.The ring-type on 003 top of pipe shaft thickens band 007
It is in contact with the inner wall of casing 004, and maintains the spatial position of pipe shaft 003.Since the ring-type of pipe shaft 003 thickens the work of band 007
With having the distance of 2mm between 004 inner wall of tube wall and casing of 003 upper end opening of pipe shaft, subsequently pipe shaft gripped with tweezers to facilitate
003.At this point, the microcentrifugation pipe pipe shaft 003 in input casing 004 is opening up, then nucleic acid purification post 001 is put into set
In pipe 004.Nucleic acid purification post 001 declines under the effect of gravity, until its lower end taper portion 008 is inserted into microcentrifugation pipe
In the opening of 003 upper end of pipe shaft.003 upper end of pipe shaft is in contact with 001 lower end umbrella constriction outer wall of nucleic acid purification post, maintains
Nucleic acid purification post 001 is in the top of pipe shaft 003.At this point, 004 lower part of casing is equipped with pipe shaft 003, top is equipped with nucleic acid purification post
001.When eluting nucleic acid, eluent 20ul, microcentrifugation pipe pipe lid are added in from the upper end opening of nucleic acid purification post 001
The coarse part 009 of 002 merging uncovered nucleic acid purification column top just seals the opening of 001 upper end of nucleic acid purification post.It will push up
The trace dna elution collection device 005 of portion's capping is placed in supercentrifuge, 10000g high speed centrifugations 60 seconds.At this point, contain DNA
Eluent just thrown away from the lower end taper portion 008 of nucleic acid purification post 001, fall directly into microcentrifugation pipe pipe shaft 003
In.Trace dna elution collection device 005 is taken out after centrifugation, top tape pipe lid is gently taken out with right hand middle finger, forefinger and thumb
002 nucleic acid purification post 001, the nucleic acid purification post that flips upside down 001.At this point, the pipe lid 002 at 001 top of nucleic acid purification post is in weight
The right hand palm of the hand is fallen under power effect, with right hand little finger of toe and the nameless pipe lid 002 for clamping the palm of the hand.Right hand forefinger, middle finger and thumb
The nucleic acid purification post 001 pinched gives discarding, then takes sharp tweezers with right hand forefinger, middle finger and thumb, presss from both sides out and has filled from casing 004
There is a microcentrifugation pipe pipe shaft 003 of eluted dna solution, left hand pinches the pipe shaft 003 of tweezers taking-up, and the right hand is by the pipe of the palm of the hand
Lid 002 screws in pipe shaft 003, and trace dna elution collection process is completed.Eluted dna solution is placed in refrigerator cold-storage and preserves for use；
Referring to Fig.2, second of embodiment of the present invention, a kind of trace dna elutes collection device, nucleic acid purification post 010, micro-
Amount be collected by centrifugation pipe pipe shaft 011 and casing 012 the mode for being assembled into micro elution collection device 013 and its disassemble mode with
Embodiment shown in FIG. 1 is identical.Different include following aspect：First, 010 upper end of nucleic acid purification post is without coarse part, and
Upper end carries lid 014, and is connected with plastic tape 015 with tube wall.Therefore, when being eluted, microcentrifugation pipe pipe lid
016 without using.Secondly, the upper part of microcentrifugation pipe pipe shaft 011 does not have screw thread, and the sealing with pipe lid 016 is
It is connected with the mode of bayonet；
Refering to Fig. 3, a kind of the third embodiment of the invention, trace dna elutes collection device, nucleic acid purification post 017, micro-
Amount be collected by centrifugation pipe pipe shaft 018 and casing 019 the mode for being assembled into micro elution collection device 020 and its disassemble mode with
Embodiment shown in FIG. 1 is identical.Different include following aspect：The larger coarse part of 017 top outer diameter of nucleic acid purification post
Cylinder 022 smaller with lower section outer diameter forms an inverted shoulder 021.After nucleic acid purification post 017 puts into casing 019, nucleic acid is pure
Change column 017 under the effect of gravity to decline, lower end buttress shaft 024 is inserted into the upper end opening 025 of microcentrifugation pipe pipe shaft 018
Portion.Since the nucleic acid purification post 017 is shorter, lower end umbrella constriction 026 does not touch the microcentrifugation of lower section
The upper end opening 025 of pipe pipe shaft 018.It is because of the coarse part shape in 017 upper end of nucleic acid purification post that nucleic acid purification post 017, which stops declining,
Into inverted shoulder 021 by frame in the opening 023 of 018 upper end of casing.
It is obvious to a person skilled in the art that the present invention is not limited to the details of above-mentioned exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Profit requirement rather than above description limit, it is intended that all by what is fallen within the meaning and scope of the equivalent requirements of the claims
Variation is included within the present invention.Any reference numeral in claim should not be considered as to the involved claim of limitation.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in each embodiment can also be properly combined, forms those skilled in the art
The other embodiment being appreciated that.
Claims (8)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810310099.3A CN108192813A (en) | 2018-04-09 | 2018-04-09 | A kind of trace dna elutes collection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810310099.3A CN108192813A (en) | 2018-04-09 | 2018-04-09 | A kind of trace dna elutes collection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108192813A true CN108192813A (en) | 2018-06-22 |
Family
ID=62596409
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810310099.3A Pending CN108192813A (en) | 2018-04-09 | 2018-04-09 | A kind of trace dna elutes collection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108192813A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113234591A (en) * | 2021-05-28 | 2021-08-10 | 宁波康程德诺生物医药有限公司 | Integrated nucleic acid rapid-extraction test tube, rapid-extraction detection device and method |
| CN113717837A (en) * | 2021-08-13 | 2021-11-30 | 通用生物(安徽)股份有限公司 | Detachable medicinal nucleic acid purification post |
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| US6177009B1 (en) * | 1998-04-03 | 2001-01-23 | Macherey, Nagel Gmbh & Co. | Apparatus for treating biomolecules |
| US20020192656A1 (en) * | 2001-06-15 | 2002-12-19 | Richardson Derrick A. | Filtration column devices and methods of filtering therewith |
| US20030069413A1 (en) * | 2001-06-15 | 2003-04-10 | Pai Derek S | Low volume filtration column devices and methods of filtering therewith |
| CN201527375U (en) * | 2009-06-16 | 2010-07-14 | 上海捷瑞生物工程有限公司 | Nucleic acid separating and purifying column |
| CN102041255A (en) * | 2009-10-19 | 2011-05-04 | 杭州优思达生物技术有限公司 | Instrument-free nucleic acid extraction device and trace nucleic acid extraction method |
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| CN208440621U (en) * | 2018-04-09 | 2019-01-29 | 杭州倍强医药科技有限公司 | A kind of trace dna elution collection device |
-
2018
- 2018-04-09 CN CN201810310099.3A patent/CN108192813A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US6177009B1 (en) * | 1998-04-03 | 2001-01-23 | Macherey, Nagel Gmbh & Co. | Apparatus for treating biomolecules |
| US20020192656A1 (en) * | 2001-06-15 | 2002-12-19 | Richardson Derrick A. | Filtration column devices and methods of filtering therewith |
| US20030069413A1 (en) * | 2001-06-15 | 2003-04-10 | Pai Derek S | Low volume filtration column devices and methods of filtering therewith |
| CN201527375U (en) * | 2009-06-16 | 2010-07-14 | 上海捷瑞生物工程有限公司 | Nucleic acid separating and purifying column |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113234591A (en) * | 2021-05-28 | 2021-08-10 | 宁波康程德诺生物医药有限公司 | Integrated nucleic acid rapid-extraction test tube, rapid-extraction detection device and method |
| CN113234591B (en) * | 2021-05-28 | 2024-03-22 | 宁波康程德诺生物医药有限公司 | Integrated nucleic acid quick-lifting test tube, quick-lifting detection device and method |
| CN113717837A (en) * | 2021-08-13 | 2021-11-30 | 通用生物(安徽)股份有限公司 | Detachable medicinal nucleic acid purification post |
| CN113717837B (en) * | 2021-08-13 | 2023-10-03 | 通用生物(安徽)股份有限公司 | Detachable medicinal nucleic acid purification column |
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