CN1081575A - The extracting method of Tea Pigment - Google Patents
The extracting method of Tea Pigment Download PDFInfo
- Publication number
- CN1081575A CN1081575A CN 92115082 CN92115082A CN1081575A CN 1081575 A CN1081575 A CN 1081575A CN 92115082 CN92115082 CN 92115082 CN 92115082 A CN92115082 A CN 92115082A CN 1081575 A CN1081575 A CN 1081575A
- Authority
- CN
- China
- Prior art keywords
- tea
- tea pigment
- extracting method
- pigment
- extraction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000049 pigment Substances 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 17
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 9
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims abstract description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000001035 drying Methods 0.000 claims abstract description 5
- 238000000926 separation method Methods 0.000 claims abstract description 4
- 238000000967 suction filtration Methods 0.000 claims abstract description 4
- 238000000605 extraction Methods 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 4
- 238000012546 transfer Methods 0.000 claims description 4
- 206010028980 Neoplasm Diseases 0.000 abstract description 8
- 239000012141 concentrate Substances 0.000 abstract description 7
- 201000011510 cancer Diseases 0.000 abstract description 6
- 229940079593 drug Drugs 0.000 abstract description 4
- 239000003814 drug Substances 0.000 abstract description 4
- 239000000463 material Substances 0.000 abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 4
- 238000001556 precipitation Methods 0.000 abstract description 3
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 abstract description 2
- 239000002537 cosmetic Substances 0.000 abstract description 2
- 229910052732 germanium Inorganic materials 0.000 abstract description 2
- GNPVGFCGXDBREM-UHFFFAOYSA-N germanium atom Chemical compound [Ge] GNPVGFCGXDBREM-UHFFFAOYSA-N 0.000 abstract description 2
- 235000013402 health food Nutrition 0.000 abstract description 2
- 238000007654 immersion Methods 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 239000011669 selenium Substances 0.000 abstract description 2
- 229910052711 selenium Inorganic materials 0.000 abstract description 2
- 239000011573 trace mineral Substances 0.000 abstract description 2
- 235000013619 trace mineral Nutrition 0.000 abstract description 2
- 239000004480 active ingredient Substances 0.000 abstract 1
- 238000009835 boiling Methods 0.000 abstract 1
- 239000002184 metal Substances 0.000 abstract 1
- 244000269722 Thea sinensis Species 0.000 description 17
- 239000000203 mixture Substances 0.000 description 8
- 239000004615 ingredient Substances 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 4
- 230000001093 anti-cancer Effects 0.000 description 3
- 238000002425 crystallisation Methods 0.000 description 3
- 230000008025 crystallization Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 2
- 231100000215 acute (single dose) toxicity testing Toxicity 0.000 description 2
- RYYVLZVUVIJVGH-UHFFFAOYSA-N caffeine Chemical compound CN1C(=O)N(C)C(=O)C2=C1N=CN2C RYYVLZVUVIJVGH-UHFFFAOYSA-N 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002481 ethanol extraction Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 230000004614 tumor growth Effects 0.000 description 2
- WOAHJDHKFWSLKE-UHFFFAOYSA-N 1,2-benzoquinone Chemical compound O=C1C=CC=CC1=O WOAHJDHKFWSLKE-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000008946 Fibrinogen Human genes 0.000 description 1
- 108010049003 Fibrinogen Proteins 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
- 240000001307 Myosotis scorpioides Species 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 235000006468 Thea sinensis Nutrition 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 238000011047 acute toxicity test Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 239000001058 brown pigment Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 229940012952 fibrinogen Drugs 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 210000003630 histaminocyte Anatomy 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention provides a kind of extracting method of Tea Pigment, main technique is that the boiling water immersion concentrates active ingredient in the tealeaves is soluble in water, add ethanol then and make each component separation, add the NaOH adjust pH and make other become fractional precipitation, after adding the chloroform dewatering and defatting behind the suction filtration, be drying to obtain Tea Pigment.This product is a kind of All Pure Nature cancer-resisting material, and contains 20 kinds of trace elements and rare metal selenium, germanium etc., is the raw material of health care medication, health drink, health food, cosmetics.
Description
The present invention relates to a kind of extracting method of Tea Pigment, belong to a kind of technology of extracting effective ingredient in the tealeaves.
China is that the world produces tea big country, and tea is as the history in existing four, 5,000 years of Chinese a kind of beverage, and drinking tea is good for one's health, and has been well-known.The medical value of tea also is subject to people's attention, though the past has the people once to attempt to extract its effective ingredient and applied for patent from tealeaves, but its technology is only improved the make tea method of tea making of tradition, both do not adopted chemical method to extract, also do not extract single effective ingredient, for example application for a patent for invention 91106983.6 " caffein extracting method " is the state-of-the-art technology that we retrieve.Yet this method and the traditional mode of infusing tea do not have the difference of matter, and contained composition is a lot of in the tea, and organic component reaches 450 kinds, is not that all compositions all have equivalent effect, so be necessary the main anti-cancer composition in the tealeaves is carried out single extraction, and carry out inhibition test.
The objective of the invention is to seek nontoxic, cancer-resisting composition from natural goods, the Tea Pigment that promptly will have anti-cancer and kill cancer action from the multiple effective ingredient of tealeaves is separated, so that outstanding its mainly acts on, eliminates the harmful effect of other compositions in therapeutic process.
The present invention is achieved in that the general method of extracting effective ingredient from tealeaves mainly takes physical method, and main technique is:
Soak extraction → filtration → concentration extraction → dry several key steps.
The present invention has taked chemical method, and the main technique flow process is:
Soak and concentrate → ethanol extraction → accent pH value → suction filtration → separation and Extraction → drying.
Further illustrate feature of the present invention below in conjunction with embodiment:
1, immersion concentrates: doubly 100 ℃ of hot water gradation are soluble in water with effective ingredient in the tea with 3-4, and heating concentrates the abundant oxidation of polyphenols in the tea then;
2, ethanol extraction: add three to four-fold 95% ethanol multiple components is separated;
3, transfer pH value: add 5～20%NaOH and transfer to PH7.0～7.5 and make other compositions precipitations;
4, suction filtration: go precipitation to collect filtrate;
5, separation and Extraction: add chloroform and play dehydration, degreasing extraction Tea Pigment;
6, drying: 60 ℃ of freeze-day with constant temperature obtain Tea Pigment (TP-91) crystallization.
We extract Tea Pigment (TP-91) first and have carried out many index and observed anti-tumor experiment from tea, below in conjunction with its effect of instance analysis.
Tea Pigment is the crystallization composition that Chinese tea (Camellia Sinensis) obtains after a series of processing, contain uranidin, haematochrome and brown pigment, it chemically is positive quinone (Orthoquinone), be used for anticancer experiment through us, found that: Tea Pigment (TP-91) is to the inhibitory rate 43% of mouse S-180, and experiment in vitro is to the tumor cell line Hela cell and almost 100% death of EAC cell of cultivation.Tea Pigment (TP-91) is to the lotus knurl main blood that contracts, biochemistry, and immunity and enzyme all produce the influence that is unfavorable for tumor growth.SOD is active in the red blood cell increases, and plasma fibrinogen descends, and the mast cell number increases in the tumor tissue, and the blood clotting reduction has constituted the internal environment that is unfavorable for tumor growth and transfer, and tumour is subjected to inhibition.Tea Pigment is a kind of novel All Pure Nature cancer-resisting material, and contain 20 kinds of trace elements, the selenium relevant with cancer-resisting, germanium etc. are wherein arranged, can act on the health care medication, health drink, the raw material of health food or health care cosmetic product, of many uses, to ensureing people's health, cancer-resisting has positive role.
Tea Pigment (TP-91) acute toxicity testing
Tea Pigment (TP-91) is the crystallization composition that obtains after a series of processing from Chinese tea (Camllia Sinensis), observe Tea Pigment (TP-91) to the chmice acute toxic action, so that whether understand heavy dose of medication toxic, be experimental applications and clinical safety evaluatio and the experimental basis of providing.
Material and method
Tea Pigment (TP-91) extracts from Chinese tea, this chamber self-control.
The Aqua Folium Camelliae sinensis concentrate contrasts with Tea Pigment (TP-91).
Drug dose: it respectively is 400 times (human body 15mg/kg/ days) of people's body and function amount that Tea Pigment and tea concentrate, and once irritates stomach, observes a week.
Animal: healthy kunming mice 18-22 gram, male and female have concurrently, are 10 groups with animal with deduction of points, 5 groups of Tea Pigments, 5 groups of tea concentrates.
The result
In this experiment 400 times of maximum intelligent body and function amount find dead, but tealeaves when concentrating maximum dose level mouse jump action appears, 3-5 restores calm second, observes to the 3rd day part mouse ear and finds the tip-like blutpunkte, dies down after the week.And Tea Pigment group (TP-91) maximum dose is not seen abnormal conditions.The result shows that Tea Pigment (TP-91) coefficient safe in utilization is big, and no abnormal reaction in the acute toxicity test thinks that Tea Pigment (TP-91) is All Pure Nature material, laboratory applications and clinical practice safety.
Claims (1)
1, a kind of extracting method of Tea Pigment, its main technique flow process is: soak concentrated → chemical extraction → accent pH value → suction filtration → separation and Extraction → drying, wherein chemical extraction is the ethanol that adds three to four-fold 95%, transferring pH value is to add NaOH to make pH value transfer to 7.0～7.5, separation-extraction technology adds chloroform, and drying process is at 60 ℃ of constant temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92115082 CN1031309C (en) | 1992-12-29 | 1992-12-29 | Extraction method of tea pigment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92115082 CN1031309C (en) | 1992-12-29 | 1992-12-29 | Extraction method of tea pigment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1081575A true CN1081575A (en) | 1994-02-09 |
| CN1031309C CN1031309C (en) | 1996-03-20 |
Family
ID=4947256
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 92115082 Expired - Fee Related CN1031309C (en) | 1992-12-29 | 1992-12-29 | Extraction method of tea pigment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1031309C (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1035660C (en) * | 1993-01-01 | 1997-08-20 | 江西省绿色工业(集团)公司 | Tea pigment and preparing process thereof |
| CN1078472C (en) * | 1997-08-08 | 2002-01-30 | 江西省绿色工业集团公司 | Application of theachrome in preparing medicine for plateau erythrocytosis |
| CN1304586C (en) * | 2003-12-13 | 2007-03-14 | 无锡市世纪生物药业有限公司 | Method for preparing tea pigment |
| CN101731390B (en) * | 2009-12-24 | 2012-05-23 | 应维强 | Production process of tea residue extract |
-
1992
- 1992-12-29 CN CN 92115082 patent/CN1031309C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1035660C (en) * | 1993-01-01 | 1997-08-20 | 江西省绿色工业(集团)公司 | Tea pigment and preparing process thereof |
| CN1078472C (en) * | 1997-08-08 | 2002-01-30 | 江西省绿色工业集团公司 | Application of theachrome in preparing medicine for plateau erythrocytosis |
| CN1304586C (en) * | 2003-12-13 | 2007-03-14 | 无锡市世纪生物药业有限公司 | Method for preparing tea pigment |
| CN101731390B (en) * | 2009-12-24 | 2012-05-23 | 应维强 | Production process of tea residue extract |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1031309C (en) | 1996-03-20 |
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| C14 | Grant of patent or utility model | ||
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