CN107926929A - A kind of Cryopreservation of Sungkiang weever sperm - Google Patents
A kind of Cryopreservation of Sungkiang weever sperm Download PDFInfo
- Publication number
- CN107926929A CN107926929A CN201711212448.XA CN201711212448A CN107926929A CN 107926929 A CN107926929 A CN 107926929A CN 201711212448 A CN201711212448 A CN 201711212448A CN 107926929 A CN107926929 A CN 107926929A
- Authority
- CN
- China
- Prior art keywords
- sperm
- semen
- sucrose
- methanol
- cryopreservation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 238000005138 cryopreservation Methods 0.000 title claims abstract description 8
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 24
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 19
- 238000000034 method Methods 0.000 claims abstract description 17
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims abstract description 14
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 8
- 229930006000 Sucrose Natural products 0.000 claims abstract description 8
- 235000011187 glycerol Nutrition 0.000 claims abstract description 8
- 239000005720 sucrose Substances 0.000 claims abstract description 8
- 239000001103 potassium chloride Substances 0.000 claims abstract description 7
- 235000011164 potassium chloride Nutrition 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 239000012153 distilled water Substances 0.000 claims abstract description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 4
- 238000004321 preservation Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000007710 freezing Methods 0.000 claims 1
- 230000008014 freezing Effects 0.000 claims 1
- 230000001681 protective effect Effects 0.000 claims 1
- 210000000582 semen Anatomy 0.000 abstract description 12
- 241000269799 Perca fluviatilis Species 0.000 abstract description 6
- 238000009360 aquaculture Methods 0.000 abstract description 4
- 244000144974 aquaculture Species 0.000 abstract description 4
- 239000000243 solution Substances 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 4
- 239000002577 cryoprotective agent Substances 0.000 abstract description 3
- 239000003761 preservation solution Substances 0.000 abstract description 3
- ONGZMKPEWCAXFS-UHFFFAOYSA-L [Cl+].[Cl-].[K+].[Cl-] Chemical compound [Cl+].[Cl-].[K+].[Cl-] ONGZMKPEWCAXFS-UHFFFAOYSA-L 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
- A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/16—Physical preservation processes
- A01N1/162—Temperature processes, e.g. following predefined temperature changes over time
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明属于水产养殖行业，具体是养殖行业所使用的一种松江鲈鱼精液的低温保存方法。其特征是：在采用本方法进行精液保存精液时，应先稀释精液，本方法所述的保存液组成为，蔗糖、氯化钾、甲醇、在液氮蒸汽中冷冻，并且添加甘油作为额外的冷冻保护剂。冷冻保存液组成，采用重量百分比，蔗糖0.05‑0.5％，氯化钾为0.01‑0.15％，甲醇为6‑12％，甘油为0.05‑5％，其余含量由蒸馏水补齐。综上所述，本发明大大提高精子的成活率，使用操作简单。The invention belongs to the aquaculture industry, in particular to a low-temperature preservation method for Songjiang perch semen used in the aquaculture industry. It is characterized in that: when using this method to preserve semen, the semen should be diluted first. The preservation solution described in this method is composed of sucrose, potassium chloride, methanol, frozen in liquid nitrogen vapor, and glycerin is added as an additional Cryoprotectant. The cryopreservation solution is composed of 0.05-0.5% sucrose, 0.01-0.15% potassium chloride, 6-12% methanol, 0.05-5% glycerol, and distilled water to make up the rest. In summary, the present invention greatly improves the survival rate of sperm, and is easy to use and operate.
Description
技术领域：Technical field:
本发明属于水产养殖行业，具体是养殖行业所使用的一种松江鲈鱼精液的低温保存方法。The invention belongs to the aquaculture industry, in particular to a low-temperature preservation method for Songjiang perch semen used in the aquaculture industry.
背景技术：Background technique:
目前的松江鲈鱼精液的低温保存方法，普遍存在着方法复杂，使用很不方便，精子的成活率低等缺点。The current cryopreservation method of perch semen in Songjiang generally has the disadvantages of complicated method, inconvenient use, and low survival rate of sperm.
发明内容：Invention content:
本发明的目的，就是为了解决上述的问题，而研发一种松江鲈鱼精液的低温保存方法。The purpose of the present invention is to solve the above-mentioned problems, and develop a method for cryopreservation of Songjiang perch semen.
本发明所要解决的技术方案是：本发明涉及一种松江鲈鱼精液的低温保存方法，其特征是：在采用本方法进行精液保存精液时，应先稀释精液，本方法所述的保存液组成为，蔗糖、氯化钾、甲醇、在液氮蒸汽中冷冻，并且添加甘油作为额外的冷冻保护剂。冷冻保存液组成，采用重量百分比，蔗糖0.05-0.5％，氯化钾为0.01-0.15％，甲醇为6-12％，甘油为0.05-5％，其余含量由蒸馏水补齐。The technical solution to be solved by the present invention is: the present invention relates to a method for cryopreservation of Songjiang perch semen, which is characterized in that: when using this method to preserve semen, the semen should be diluted first, and the preservation solution described in this method consists of , sucrose, potassium chloride, methanol, frozen in liquid nitrogen vapor, and added glycerol as an additional cryoprotectant. The cryopreservation solution is composed of 0.05-0.5% of sucrose, 0.01-0.15% of potassium chloride, 6-12% of methanol, 0.05-5% of glycerin, and the remaining content is made up by distilled water.
本发明产生的积极效果：本方法能够大大提高精子的成活率。The positive effect produced by the invention: the method can greatly improve the survival rate of sperm.
具体实施方式：Detailed ways:
本发明涉及一种松江鲈鱼精液的低温保存方法，在采用本方法进行精液保存精液时，应先在100毫升的玻璃容器内稀释精液，本方法所述的保存液组成为，蔗糖10毫升、氯化钾6毫升、甲醇5毫升、在液氮蒸汽中冷冻，并且添加甘油25毫升，作为额外的冷冻保护剂。冷冻保存液组成，采用重量百分比，蔗糖0.05-0.5％，氯化钾为0.01-0.15％，甲醇为6-12％，甘油为0.05-5％，其余含量由蒸馏水补齐。The invention relates to a low-temperature preservation method for the semen of Songjiang perch. When adopting the method to preserve the semen, the semen should be diluted in a glass container of 100 milliliters. The preservation solution described in the method consists of 10 milliliters of sucrose, chlorine Potassium chloride 6 ml, methanol 5 ml, frozen in liquid nitrogen vapor, and glycerol 25 ml added as an additional cryoprotectant. The cryopreservation solution is composed of 0.05-0.5% of sucrose, 0.01-0.15% of potassium chloride, 6-12% of methanol, 0.05-5% of glycerin, and the remaining content is made up by distilled water.
综上所述，本发明大大提高精子的成活率，使用操作简单。In summary, the present invention greatly improves the survival rate of sperm, and is easy to use and operate.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711212448.XA CN107926929A (en) | 2017-11-27 | 2017-11-27 | A kind of Cryopreservation of Sungkiang weever sperm |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711212448.XA CN107926929A (en) | 2017-11-27 | 2017-11-27 | A kind of Cryopreservation of Sungkiang weever sperm |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107926929A true CN107926929A (en) | 2018-04-20 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711212448.XA Withdrawn CN107926929A (en) | 2017-11-27 | 2017-11-27 | A kind of Cryopreservation of Sungkiang weever sperm |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107926929A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109122668A (en) * | 2018-10-25 | 2019-01-04 | 镇江市绿色农业科技有限公司 | A kind of store method of hilsa herring sperm |
| CN114271264A (en) * | 2022-01-11 | 2022-04-05 | 新疆农业大学 | A kind of cod semen cryopreservation solution, preparation method and use thereof |
| CN115362961A (en) * | 2022-04-14 | 2022-11-22 | 中国水产科学研究院北戴河中心实验站 | Artificial insemination method for Trachidermus fasciatus and application thereof |
-
2017
- 2017-11-27 CN CN201711212448.XA patent/CN107926929A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109122668A (en) * | 2018-10-25 | 2019-01-04 | 镇江市绿色农业科技有限公司 | A kind of store method of hilsa herring sperm |
| CN114271264A (en) * | 2022-01-11 | 2022-04-05 | 新疆农业大学 | A kind of cod semen cryopreservation solution, preparation method and use thereof |
| CN115362961A (en) * | 2022-04-14 | 2022-11-22 | 中国水产科学研究院北戴河中心实验站 | Artificial insemination method for Trachidermus fasciatus and application thereof |
| CN115362961B (en) * | 2022-04-14 | 2023-12-08 | 中国水产科学研究院北戴河中心实验站 | Artificial insemination method of Songjiang perch and application thereof |
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Legal Events
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| PB01 | Publication | ||
| PB01 | Publication | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180420 |