CN107907396A - A kind of biological tissue's active immunity histochemical staining system - Google Patents
A kind of biological tissue's active immunity histochemical staining system Download PDFInfo
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- 238000010186 staining Methods 0.000 title claims abstract description 32
- 230000001744 histochemical effect Effects 0.000 title claims 5
- 230000036039 immunity Effects 0.000 title claims 5
- 239000007788 liquid Substances 0.000 claims abstract description 120
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims abstract description 54
- 239000011521 glass Substances 0.000 claims abstract description 50
- 239000000243 solution Substances 0.000 claims abstract description 31
- 239000007977 PBT buffer Substances 0.000 claims abstract description 21
- 238000004043 dyeing Methods 0.000 claims abstract description 19
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- 238000011532 immunohistochemical staining Methods 0.000 abstract description 11
- 238000002474 experimental method Methods 0.000 abstract description 8
- 230000002055 immunohistochemical effect Effects 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 6
- 238000003364 immunohistochemistry Methods 0.000 abstract description 2
- 239000007853 buffer solution Substances 0.000 description 3
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- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 2
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Abstract
一种生物组织自动免疫组化染色系统，涉及生物组织培养设备。本发明解决现有免疫组化实验人工操作，耗时费力，且不适用于较厚的生物组织免疫组化的技术问题。本发明包括玻璃染色管、一抗储液罐、二抗储液罐、PBT缓冲溶液储液罐、甲醛固定液储液罐、进液管、出液管、若干连接管和废液罐；所述玻璃染色管一端开口，开口处设有密封塞，所述密封塞上设有进液孔和出液孔，所述玻璃染色管腔内底侧设有出液管保护腔，所述出液管保护腔侧壁上设有若干通孔。本发明采用特制的玻璃染色管，可将较厚的生物组织放在染色腔内进行免疫组化染色实验，并自动完成免疫组化流程。
An automatic immunohistochemical staining system for biological tissue relates to biological tissue culture equipment. The invention solves the technical problems of manual operation of existing immunohistochemical experiments, which is time-consuming and laborious, and is not suitable for thick biological tissue immunohistochemistry. The invention comprises a glass dyeing tube, a primary antibody storage tank, a secondary antibody storage tank, a PBT buffer solution storage tank, a formaldehyde fixative liquid storage tank, a liquid inlet pipe, a liquid outlet pipe, several connecting pipes and a waste liquid tank; One end of the glass dyeing tube is open, and a sealing plug is provided at the opening, and a liquid inlet hole and a liquid outlet hole are arranged on the sealing plug, and a liquid outlet pipe protection cavity is provided on the bottom side of the glass dyeing tube cavity, and the liquid outlet A number of through holes are arranged on the side wall of the tube protection chamber. The invention adopts a special glass staining tube, which can place thicker biological tissues in the staining cavity for immunohistochemical staining experiments, and automatically completes the immunohistochemical process.
Description
技术领域technical field
本发明属于生物组织培养设备技术领域，特别涉及一种生物组织自动免疫组化染色系统。The invention belongs to the technical field of biological tissue culture equipment, in particular to an automatic immunohistochemical staining system for biological tissue.
背景技术Background technique
免疫组化，是应用免疫学基本原理——抗原抗体反应，即抗原与抗体特异性结合的原理，通过化学反应使标记抗体的显色剂荧光素、酶、金属离子、同位素显色来确定组织细胞内抗原多肽和蛋白质，对其进行定位、定性及定量的研究。Immunohistochemistry is the application of the basic principle of immunology—antigen-antibody reaction, that is, the principle of specific combination of antigen and antibody. Through chemical reaction, the chromogenic agent fluorescein, enzyme, metal ion, and isotope of the labeled antibody are developed to determine the color of the tissue. Intracellular antigenic polypeptides and proteins, for localization, qualitative and quantitative research.
免疫组化实验整个流程不仅步骤繁琐，而且每隔一定的时间，需要人工加样和换洗液体，耗时费力，易出现加样量不精确等缺点。The whole process of immunohistochemical experiment is not only cumbersome, but also requires manual sample addition and liquid replacement at regular intervals, which is time-consuming and labor-intensive, and is prone to shortcomings such as inaccurate sample volume.
一种采用特制载玻片扁平空间的果蝇翅芽自动免疫组化装置，虽然能够自动操作，但只能用于较薄生物组织的免疫组化染色实验，不适用于较厚的生物组织，例如果蝇的大脑组织，就不能用载玻片扁平空间进行免疫组化染色实验。An automatic immunohistochemical device for Drosophila wing buds that uses a special glass slide flat space. Although it can be operated automatically, it can only be used for immunohistochemical staining experiments on thin biological tissues, not for thicker biological tissues. For example, the brain tissue of Drosophila cannot be used for immunohistochemical staining experiments in the flat space of glass slides.
发明内容Contents of the invention
本发明的目的在于克服现有技术的缺点，提供一种生物组织自动免疫组化染色系统，解决现有免疫组化实验人工操作，耗时费力，且不适用于较厚的生物组织免疫组化的技术问题。The purpose of the present invention is to overcome the shortcomings of the prior art, to provide an automatic immunohistochemical staining system for biological tissue, to solve the problem of manual operation of existing immunohistochemical experiments, which is time-consuming and laborious, and is not suitable for thicker biological tissue immunohistochemical staining technical problems.
本发明是通过以下技术方案实现的：The present invention is achieved through the following technical solutions:
一种生物组织自动免疫组化染色系统，其中：包括玻璃染色管、一抗储液罐、二抗储液罐、PBT缓冲溶液储液罐、甲醛固定液储液罐、进液管、出液管、若干连接管和废液罐；An automatic immunohistochemical staining system for biological tissues, which includes glass staining tubes, primary antibody storage tanks, secondary antibody storage tanks, PBT buffer solution storage tanks, formaldehyde fixative liquid storage tanks, liquid inlet tubes, and liquid outlet pipe, several connecting pipes and waste tank;
所述玻璃染色管一端开口，所述玻璃染色管开口处设有密封塞，所述密封塞上设有进液孔和出液孔，所述玻璃染色管内底侧设有出液管保护腔；One end of the glass dyeing tube is open, a sealing plug is provided at the opening of the glass dyeing tube, a liquid inlet hole and a liquid outlet hole are provided on the sealing plug, and a liquid outlet tube protection cavity is provided on the inner bottom side of the glass dyeing tube;
所述一抗储液罐、二抗储液罐、PBT缓冲溶液储液罐和甲醛固定液储液罐设在玻璃染色管一侧上方，所述废液罐设在玻璃染色管一侧下方，所述一抗储液罐、二抗储液罐、PBT缓冲溶液储液罐、甲醛固定液储液罐的出口分别通过连接管与进液管连接，所述进液管穿过密封塞上的进液孔伸至玻璃染色管内，所述出液管的一端穿过密封塞上的出液孔伸至所述出液管保护腔内，所述出液管的另一端与废液罐连接；The primary antibody storage tank, the secondary antibody storage tank, the PBT buffer solution storage tank and the formaldehyde fixative liquid storage tank are arranged above one side of the glass staining tube, and the waste liquid tank is arranged under one side of the glass staining tube, The outlets of the primary anti-liquid storage tank, the secondary anti-liquid storage tank, the PBT buffer solution storage tank, and the formaldehyde fixative liquid storage tank are respectively connected to the liquid inlet pipe through the connecting pipe, and the liquid inlet pipe passes through the opening on the sealing plug. The liquid inlet hole extends into the glass dyeing tube, one end of the liquid outlet pipe passes through the liquid outlet hole on the sealing plug and extends into the protective chamber of the liquid outlet pipe, and the other end of the liquid outlet pipe is connected to the waste liquid tank;
所述一抗储液罐、二抗储液罐、PBT缓冲溶液储液罐、甲醛固定液储液罐与进液管之间的连接管上均设有由单片机控制模块控制的微型液体控制阀，所述出液管上设有由单片机控制模块控制的抽液泵；The connecting pipes between the primary antibody liquid storage tank, the secondary antibody liquid storage tank, the PBT buffer solution liquid storage tank, the formaldehyde fixative liquid storage tank and the liquid inlet pipe are all equipped with micro-liquid control valves controlled by the single-chip microcomputer control module , the liquid outlet pipe is provided with a liquid suction pump controlled by a single-chip microcomputer control module;
所述一抗储液罐、二抗储液罐上设有由单片机控制模块控制的低温控制装置，所述玻璃染色管上设有红外测温探头。The primary antibody liquid storage tank and the secondary antibody liquid storage tank are provided with a low-temperature control device controlled by a single-chip microcomputer control module, and an infrared temperature measuring probe is provided on the glass staining tube.
进一步，所述玻璃染色管固定在翘板摇床上，翘板摇床按一定的频率摇动；所述进液管和出液管为软管。Further, the glass dyeing tube is fixed on a rocker shaker, and the rocker shaker is shaken at a certain frequency; the liquid inlet pipe and liquid outlet pipe are hoses.
进一步，所述进液管的出口端头弯向所述玻璃染色管内壁。Further, the outlet end of the liquid inlet pipe is bent towards the inner wall of the glass dyeing pipe.
进一步，所述出液管保护腔侧壁上设有若干阻止生物组织通过的通液小孔，位于所述出液管保护腔内的出液管上设有过滤网。Further, the side wall of the protective chamber of the liquid outlet pipe is provided with a number of small liquid holes to prevent the passage of biological tissues, and the outlet pipe located in the protective chamber of the liquid outlet pipe is provided with a filter.
本发明采用特制的玻璃染色管，可将较厚的生物组织放在染色腔内进行免疫组化染色实验，并自动完成免疫组化流程；密封塞的设置可实现染色管的密封，避免外界微生物侵染培养液；一抗储液罐、二抗储液罐、PBT缓冲溶液储液罐、甲醛固定液储液罐、连接管、微型液体控制阀、进液管、出液管和废液罐的设置，可根据需要对染色管内的培养液进行更换；特别是在甲醛固定液、一抗、二抗、PBT缓冲溶液、储液罐与进液管之间设置了微型液体控制阀，并在出液管上置了抽液泵，可实现自动操作；一抗、二抗储液罐上低温控制装置的设置，确保抗体在染色过程中不变质。玻璃染色管上设有温控系统，确保一抗孵育在低温进行，其他步骤在室温左右进行。The invention adopts a special glass staining tube, which can place thicker biological tissues in the staining chamber for immunohistochemical staining experiments, and automatically completes the immunohistochemical process; the setting of the sealing plug can realize the sealing of the staining tube to avoid external microorganisms Infection culture medium; primary antibody storage tank, secondary antibody storage tank, PBT buffer solution storage tank, formaldehyde fixative solution storage tank, connecting tube, micro liquid control valve, liquid inlet pipe, liquid outlet pipe and waste liquid tank The culture solution in the staining tube can be replaced according to the needs; especially, a micro-liquid control valve is set between the formaldehyde fixative, primary antibody, secondary antibody, PBT buffer solution, liquid storage tank and liquid inlet tube, and in the A suction pump is installed on the liquid outlet pipe to realize automatic operation; the low temperature control device on the primary antibody and secondary antibody storage tanks ensures that the antibodies will not deteriorate during the staining process. A temperature control system is installed on the glass staining tube to ensure that the primary antibody incubation is carried out at low temperature, and other steps are carried out at room temperature.
附图说明Description of drawings
图1是一种生物组织自动免疫组化染色系统的结构示意图；Fig. 1 is a structural schematic diagram of a biological tissue automatic immunohistochemical staining system;
图2是玻璃染色管的结构示意图；Fig. 2 is the structural representation of glass staining tube;
图3是图2的右视图；Fig. 3 is the right view of Fig. 2;
图4是单片机控制电路原理框图。Figure 4 is a block diagram of the microcontroller control circuit.
具体实施方式Detailed ways
下面结合附图和实施例对本发明作进一步的详细描述。The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.
如图1至图3所示，本实施例中的一种生物组织自动免疫组化染色系统，包括玻璃染色管1、一抗储液罐10、二抗储液罐11、PBT缓冲溶液储液罐12、甲醛固定液储液罐13、进液管4、出液管5、若干连接管3和废液罐6；As shown in Figures 1 to 3, a biological tissue automatic immunohistochemical staining system in this embodiment includes a glass staining tube 1, a primary antibody storage tank 10, a secondary antibody storage tank 11, and a PBT buffer solution storage solution Tank 12, formaldehyde fixative liquid storage tank 13, liquid inlet pipe 4, liquid outlet pipe 5, several connecting pipes 3 and waste liquid tank 6;
所述玻璃染色管1一端开口，所述玻璃染色管1开口处设有密封塞7，所述密封塞7上设有进液孔8和出液孔9；One end of the glass staining tube 1 is open, and the opening of the glass staining tube 1 is provided with a sealing plug 7, and the sealing plug 7 is provided with a liquid inlet hole 8 and a liquid outlet hole 9;
为了防止出液时，生物组织被吸到出液管内，所述玻璃染色管1内底侧设有出液管保护腔2；In order to prevent the biological tissue from being sucked into the liquid outlet pipe when the liquid is discharged, the bottom side of the glass staining tube 1 is provided with a liquid discharge pipe protection chamber 2;
所述一抗储液罐10、二抗储液罐11、PBT缓冲溶液储液罐12和甲醛固定液储液罐13设在玻璃染色管1一侧上方，所述废液罐6设在玻璃染色管1一侧下方，所述一抗储液罐10、二抗储液罐11、PBT缓冲溶液储液罐12、甲醛固定液储液罐13的出口分别通过连接管3与进液管4连接，所述进液管4穿过密封塞7上的进液孔8伸至玻璃染色管1内，所述出液管5的一端穿过密封塞7上的出液孔9伸至所述出液管保护腔2内，所述出液管5的另一端与废液罐6连接；The primary antibody storage tank 10, the secondary antibody storage tank 11, the PBT buffer solution storage tank 12 and the formaldehyde fixative liquid storage tank 13 are arranged above the glass staining tube 1 side, and the waste liquid tank 6 is arranged on the glass staining tube 1. Below one side of the staining tube 1, the outlets of the primary antibody storage tank 10, the secondary antibody storage tank 11, the PBT buffer solution storage tank 12, and the formaldehyde fixative solution storage tank 13 respectively pass through the connecting pipe 3 and the liquid inlet pipe 4 connected, the liquid inlet pipe 4 passes through the liquid inlet hole 8 on the sealing plug 7 and extends into the glass dyeing tube 1, and one end of the liquid outlet pipe 5 passes through the liquid outlet hole 9 on the sealing plug 7 and extends to the In the liquid outlet pipe protection cavity 2, the other end of the liquid outlet pipe 5 is connected to the waste liquid tank 6;
所述一抗储液罐10、二抗储液罐11、PBT缓冲溶液储液罐12、甲醛固定液储液罐13与进液管4之间的连接管3上均设有由单片机控制模块控制的微型液体控制阀14，所述出液管5上设有由单片机控制模块控制的抽液泵15；The first anti-liquid storage tank 10, the secondary anti-liquid storage tank 11, the PBT buffer solution liquid storage tank 12, the formaldehyde fixative liquid storage tank 13 and the connection pipe 3 between the liquid inlet pipe 4 are all provided with a single-chip microcomputer control module. Controlled micro-liquid control valve 14, the liquid outlet pipe 5 is provided with a liquid suction pump 15 controlled by a single-chip microcomputer control module;
所述一抗储液罐10、二抗储液罐11上设有由单片机控制模块控制的低温控制装置，所述玻璃染色管1上设有红外测温探头17。The primary antibody storage tank 10 and the secondary antibody storage tank 11 are provided with a low-temperature control device controlled by a single-chip microcomputer control module, and an infrared temperature measuring probe 17 is provided on the glass staining tube 1 .
所述玻璃染色管1固定在翘板摇床16上，翘板摇床按一定的频率摇动；所述进液管4和出液管5为软管，不影响摇床的摇动。The glass dyeing tube 1 is fixed on a rocker shaker 16, and the rocker shaker shakes at a certain frequency; the liquid inlet pipe 4 and the liquid outlet pipe 5 are flexible pipes, which do not affect the shaking of the shaker.
所述进液管4的出口端头弯向所述玻璃染色管1内壁，防止进液时，液体直接冲刷生物组织造成破坏。The outlet end of the liquid inlet pipe 4 is bent to the inner wall of the glass dyeing pipe 1 to prevent the liquid from directly washing away the biological tissue and causing damage when the liquid is fed in.
所述出液管保护腔2侧壁上设有若干阻止生物组织通过的通液小孔，位于所述出液管保护腔2内的出液管上设有过滤网，确保液体被顺利抽出玻璃染色管。The side wall of the outlet pipe protection chamber 2 is provided with a number of liquid-through holes that prevent the passage of biological tissues, and the outlet pipe located in the outlet pipe protection chamber 2 is provided with a filter to ensure that the liquid is smoothly drawn out of the glass. Stain tube.
如图4所示，所述红外测温探头17与单片机控制模块连接，实时测量玻璃染色管1内的温度，所述一抗储液罐10、二抗储液罐11的低温控制装置与单片机控制模块连接，红外测温探头所测数据反馈给单片机，单片机再控制一抗储液罐10、二抗储液罐11的低温控制装置，从而确保一抗孵育在4～6℃进行，其他步骤在25℃左右进行。As shown in Figure 4, the infrared temperature measuring probe 17 is connected with the single-chip microcomputer control module to measure the temperature in the glass dyeing tube 1 in real time, and the low-temperature control device of the first anti-liquid storage tank 10 and the secondary anti-liquid storage tank 11 is connected with the single-chip microcomputer The control module is connected, the data measured by the infrared temperature measuring probe is fed back to the single-chip microcomputer, and the single-chip microcomputer controls the low-temperature control device of the primary antibody liquid storage tank 10 and the secondary antibody liquid storage tank 11, so as to ensure that the incubation of the primary antibody is carried out at 4-6 °C. Other steps Carry out at around 25°C.
本发明的使用过程：The use process of the present invention:
使用时，将在磷酸缓冲液中解剖下来的翅芽转移到玻璃染色管1内，塞紧密封塞7，并将玻璃染色管1固定在翘板摇床16上，翘板摇床按一定的频率摇动；When in use, the wing buds dissected in phosphate buffer solution are transferred to the glass staining tube 1, the sealing plug 7 is tightly plugged, and the glass staining tube 1 is fixed on the rocker shaker 16, and the rocker shaker is pressed at a certain frequency shaking;
通过单片机控制电路：Through the microcontroller control circuit:
首先打开甲醛固定液储液罐13下方连接管上的微型液体控制阀14和抽液泵15，确保玻璃染色管1充有一定量的固定液，然后关闭甲醛固定液储液罐13下方连接管上的微型液体控制阀14和抽液泵15，固定40～60min；First open the micro liquid control valve 14 and the pump 15 on the connection pipe below the formaldehyde fixative liquid storage tank 13 to ensure that the glass staining tube 1 is filled with a certain amount of fixative liquid, then close the formaldehyde fixative liquid storage tank 13 on the lower connection pipe The miniature liquid control valve 14 and the suction pump 15 are fixed for 40-60 minutes;
然后，开启PBT缓冲溶液储液罐12下方连接管上的微型液体控制阀14和抽液泵15，缓冲液持续清洗甲醛固定液1～1.5h后，关闭PBT缓冲溶液储液罐12下方连接管上的微型液体控制阀14和抽液泵15；Then, open the micro-liquid control valve 14 and the liquid pump 15 on the connecting pipe below the PBT buffer solution storage tank 12, and after the buffer solution continues to clean the formaldehyde fixative for 1 to 1.5 hours, close the connecting pipe below the PBT buffer solution storage tank 12 The miniature liquid control valve 14 and the suction pump 15 on the top;
同时开启一抗储液罐10下方连接管上的微型液体控制阀14和抽液泵15，确保足量的一抗溶液填充玻璃染色管1并充分浸泡生物组织后，关闭一抗储液罐10下方连接管上的微型液体控制阀14和抽液泵15，孵育8～12h。单片机控制玻璃染色管的温度在4-6℃。Simultaneously open the micro liquid control valve 14 and the pump 15 on the connecting pipe below the primary antibody storage tank 10 to ensure that the glass staining tube 1 is filled with sufficient primary antibody solution and fully soak the biological tissue, then close the primary antibody storage tank 10 Connect the micro-liquid control valve 14 and the liquid suction pump 15 on the lower connection tube, and incubate for 8-12 hours. The temperature of the glass staining tube is controlled by a single-chip computer at 4-6°C.
再次开启PBT缓冲溶液储液罐12下方连接管上的微型液体控制阀14和抽液泵15，缓冲液持续清洗一抗溶液1～1.5h后，关闭PBT缓冲溶液储液罐12下方连接管上的微型液体控制阀14和抽液泵15；Open the micro liquid control valve 14 and pump 15 on the connecting pipe below the PBT buffer solution storage tank 12 again, and after the buffer solution continues to wash the primary antibody solution for 1-1.5 hours, close the connecting pipe below the PBT buffer solution storage tank 12 The miniature liquid control valve 14 and the suction pump 15;
同时开启二抗储液罐11下方连接管上的微型液体控制阀14和出液管5上的抽液泵15，确保一定量的二抗溶液填充玻璃染色管1并充分浸泡生物组织后，关闭二抗储液罐10下方连接管上的微型液体控制阀14和抽液泵15，孵育1～1.2h；Simultaneously turn on the micro liquid control valve 14 on the connecting pipe below the secondary antibody storage tank 11 and the pump 15 on the outlet pipe 5 to ensure that a certain amount of secondary antibody solution fills the glass staining tube 1 and fully soaks the biological tissue, then close The micro liquid control valve 14 and the pump 15 on the connecting pipe below the secondary antibody liquid storage tank 10 are incubated for 1-1.2 hours;
最后开启PBT缓冲溶液储液罐12下方连接管上的微型液体控制阀14和出液管5上的抽液泵15，缓冲液持续清洗二抗溶液1～1.5h后，关闭PBT缓冲溶液储液罐12下方连接管上的微型液体控制阀14和抽液泵15，完成实验。Finally, turn on the micro liquid control valve 14 on the connecting pipe below the PBT buffer solution storage tank 12 and the pump 15 on the outlet pipe 5, and after the buffer solution continues to wash the secondary antibody solution for 1 to 1.5 hours, close the PBT buffer solution storage solution The miniature liquid control valve 14 and the suction pump 15 on the connection pipe below the tank 12 complete the experiment.
所述密封塞7塞紧玻璃染色管1后，玻璃染色管1管腔、进液管4、出液管5形成密闭的空间，以便抽液泵15可以将玻璃染色管1腔内的液体抽走。After the glass dyeing tube 1 is tightly plugged by the sealing plug 7, the glass dyeing tube 1 lumen, the liquid inlet pipe 4, and the liquid outlet pipe 5 form an airtight space, so that the liquid pump 15 can pump the liquid in the glass dyeing tube 1 cavity. Walk.
本发明能够以多种形式具体实施而不脱离发明的精神或实质，所以应当理解，上述实施例不限于前述的细节，而应在权利要求所限定的范围内广泛地解释，因此落入权利要求或其等效范围内的变化和改型都应为权利要求所涵盖。The present invention can be embodied in many forms without departing from the spirit or essence of the invention, so it should be understood that the above-described embodiments are not limited to the foregoing details, but should be interpreted broadly within the scope defined by the claims, so fall into the scope of the claims. Changes and modifications within the equivalent range thereof shall be covered by the claims.
Claims (4)
- A kind of 1. biological tissue's active immunity histochemical staining system, it is characterised in that：Including glass decorating pipe (1), primary antibody liquid storage Tank (10), secondary antibody fluid reservoir (11), PBT buffer solutions fluid reservoir (12), formaldehyde fixer fluid reservoir (13), inlet tube (4), go out Liquid pipe (5), some connecting tubes (3) and waste liquid tank (6)；Glass decorating pipe (1) one end open, glass decorating pipe (1) opening are equipped with sealing-plug (7), the sealing-plug (7) inlet opening (8) and fluid hole (9) are equipped with, the interior bottom side of the glass decorating pipe (1) is equipped with outlet tube protection chamber (2)；The primary antibody fluid reservoir (10), secondary antibody fluid reservoir (11), PBT buffer solutions fluid reservoir (12) and formaldehyde fixer fluid reservoir (13) it is located above glass decorating pipe (1) side, the waste liquid tank (6) is located at (1) one side-lower of glass decorating pipe, the primary antibody The outlet point of fluid reservoir (10), secondary antibody fluid reservoir (11), PBT buffer solutions fluid reservoir (12), formaldehyde fixer fluid reservoir (13) It is not connected by connecting tube (3) with inlet tube (4), the inlet tube (4) extends glass through the inlet opening (8) on sealing-plug (7) In glass Dyeing pipe (1), one end of the outlet tube (5) extends the outlet tube protection through the fluid hole (9) on sealing-plug (7) In chamber (2), the other end of the outlet tube (5) is connected with waste liquid tank (6)；The primary antibody fluid reservoir (10), secondary antibody fluid reservoir (11), PBT buffer solutions fluid reservoir (12), formaldehyde fixer fluid reservoir (13) the minisize liquid control valve controlled by single chip control module is equipped with the connecting tube (3) between inlet tube (4) (14), the outlet tube (5) is equipped with the drawing liquid pump (15) controlled by single chip control module；The primary antibody fluid reservoir (10), secondary antibody fluid reservoir (12) are equipped with controls dress by the low temperature of single chip control module control Put, the glass decorating pipe (1) is equipped with infrared temperature probe (17).
- A kind of 2. biological tissue's active immunity histochemical staining system according to claim 1, it is characterised in that：The glass Dyeing pipe (1) is fixed on wrap board shaking table (16), and wrap board shaking table is shaken by certain frequency；The inlet tube (4) and outlet tube (5) it is hose.
- A kind of 3. biological tissue's active immunity histochemical staining system according to claim 1, it is characterised in that：The feed liquor The outlet termination of pipe (4) bends towards the Dyeing pipe (1) inner wall.
- A kind of 4. biological tissue's active immunity histochemical staining system according to claim 1, it is characterised in that：It is described go out liquid Protection of pipe chamber (2) side wall is equipped with the logical liquid aperture that some prevention biological tissues pass through, in outlet tube protection chamber (2) Outlet tube be equipped with filter screen.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020238769A1 (en) * | 2019-05-24 | 2020-12-03 | 南京金斯瑞生物科技有限公司 | Biological reaction apparatus and method for performing biological detection on basis of apparatus |
| CN118534107A (en) * | 2024-05-08 | 2024-08-23 | 重庆斯成锐科技有限公司 | Antibody incubation device and method of full-automatic antibody hybridization instrument |
Citations (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04269641A (en) * | 1991-02-25 | 1992-09-25 | Shimadzu Corp | Suspension preparing apparatus |
| JP2001033363A (en) * | 1999-07-23 | 2001-02-09 | Nippon Koatekku:Kk | Smear-preparing device |
| CN2444230Y (en) * | 2000-05-16 | 2001-08-22 | 陈智 | Immunohistochemistry apparatus |
| CN2543056Y (en) * | 2002-05-20 | 2003-04-02 | 余向东 | Immunohistochemistry dyeing automatic controller |
| CN1445003A (en) * | 2003-04-18 | 2003-10-01 | 四川大学华西医院 | Method and device for producing bio-derived material |
| DE10158645C2 (en) * | 2001-11-22 | 2003-12-04 | Jerini Ag | Tub-shaped incubation vessel |
| US20040121456A1 (en) * | 2002-10-31 | 2004-06-24 | Fischer Andrew H. | Rapid cell block embedding method and apparatus |
| US20040266015A1 (en) * | 2002-12-20 | 2004-12-30 | Dakocytomation Denmark A/S | Automated sample processing apparatus and a method of automated treating of samples and use of such apparatus |
| CN101294903A (en) * | 2008-06-03 | 2008-10-29 | 武汉大学 | Method for detecting the expression of the same antigen on the same specimen |
| JP2009276214A (en) * | 2008-05-15 | 2009-11-26 | Hitachi High-Technologies Corp | Immuno-analyzer |
| WO2010024485A1 (en) * | 2008-08-29 | 2010-03-04 | Korea Advanced Institute Of Science And Technology | Apparatus and method for multiple immunoassays on a chip |
| CN102042924A (en) * | 2010-10-30 | 2011-05-04 | 湖南省天骑医学新技术有限公司 | Dyeing and flaking method for cells or bacteria |
| CN202141618U (en) * | 2010-10-30 | 2012-02-08 | 湖南省天骑医学新技术有限公司 | Cytological and bacterial staining and preparation machine |
| US20120135458A1 (en) * | 2010-11-30 | 2012-05-31 | General Electric Company | Closed loop monitoring of automated molecular pathology system |
| CN102519775A (en) * | 2011-11-15 | 2012-06-27 | 深圳市科软科技有限公司 | Automatic slide stainer |
| CN202382985U (en) * | 2012-01-11 | 2012-08-15 | 刘振玲 | Urine collection cup with controllable urine discharge structure |
| US20120321534A1 (en) * | 2008-06-09 | 2012-12-20 | Shazi Iqbal | Sample processing system and method |
| CN202903569U (en) * | 2012-09-18 | 2013-04-24 | 湖北阳光神琦医用科技有限公司 | Dehydrating and dyeing difunctional machine |
| CN202903571U (en) * | 2012-11-30 | 2013-04-24 | 中国水产科学研究院黑龙江水产研究所 | Gel dyeing disc |
| CN203101148U (en) * | 2013-01-30 | 2013-07-31 | 中南大学 | Staining box for immunohistochemistry |
| CN203132896U (en) * | 2013-04-11 | 2013-08-14 | 王刚平 | Immunohistochemical wet box |
| CN103543058A (en) * | 2012-07-10 | 2014-01-29 | 天津百利鑫生物科技有限公司 | Liquid injection and suction method for cell slide-making dyeing machine and device thereof |
| CN203414463U (en) * | 2013-08-30 | 2014-01-29 | 海南省交通工程检测中心 | Anti-jamming protection device of dynamic water pressure washout test machine of pavement material |
| CN103575584A (en) * | 2013-08-12 | 2014-02-12 | 北京林业大学 | Gel electrophoresis dyeing device |
| US20140342395A1 (en) * | 2010-04-20 | 2014-11-20 | Eric J. Seibel | Optical projection tomography microscopy (optm) for large specimen sizes |
| CN104302301A (en) * | 2011-12-07 | 2015-01-21 | 西托维拉公司 | Method and device for sample processing |
| CN204495637U (en) * | 2015-02-07 | 2015-07-22 | 潍坊医学院 | SABC colouration box |
| CN204789085U (en) * | 2015-07-20 | 2015-11-18 | 中国人民解放军第三军医大学 | Device is used in immunohistochemical experiment dehydration, washing |
| CN105648689A (en) * | 2016-03-21 | 2016-06-08 | 罗湘春 | Wastewater recycling system for dyeing system |
| CN105699156A (en) * | 2016-02-24 | 2016-06-22 | 福州迈新生物技术开发有限公司 | Full-automatic staining instrument for staining tissue samples on glass slides and method for applying full-automatic staining instrument |
| CN105861310A (en) * | 2016-04-22 | 2016-08-17 | 福州创方医药科技有限公司 | Integral T-cell culture device and use method thereof |
| WO2016196875A1 (en) * | 2015-06-05 | 2016-12-08 | Avanbio Inc. | A component of a device, a device, and a method for purifying and testing biomolecules from biological samples |
| CN106248461A (en) * | 2016-07-21 | 2016-12-21 | 深圳市美雅洁技术股份有限公司 | A kind of ultrasound wave rapid dewatering system for pathological tissue and using method |
| CN206020152U (en) * | 2016-08-25 | 2017-03-15 | 广州市达诚医疗技术有限公司 | Detection container |
| CN106716104A (en) * | 2014-09-22 | 2017-05-24 | 株式会社日冷生物科学 | Automatic tissue dyeing apparatus and automatic tissue dyeing method |
| CN206270135U (en) * | 2016-10-18 | 2017-06-20 | 中国人民解放军第四军医大学 | A kind of new immunohistochemical staining slide apparatus for placing |
| CN206556965U (en) * | 2017-03-10 | 2017-10-13 | 黑龙江省农业科学院作物育种研究所 | A kind of simple dimethylbenzene wash tank device of suitable paraffin section |
-
2017
- 2017-11-07 CN CN201711087020.7A patent/CN107907396B/en not_active Expired - Fee Related
Patent Citations (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04269641A (en) * | 1991-02-25 | 1992-09-25 | Shimadzu Corp | Suspension preparing apparatus |
| JP2001033363A (en) * | 1999-07-23 | 2001-02-09 | Nippon Koatekku:Kk | Smear-preparing device |
| CN2444230Y (en) * | 2000-05-16 | 2001-08-22 | 陈智 | Immunohistochemistry apparatus |
| DE10158645C2 (en) * | 2001-11-22 | 2003-12-04 | Jerini Ag | Tub-shaped incubation vessel |
| CN2543056Y (en) * | 2002-05-20 | 2003-04-02 | 余向东 | Immunohistochemistry dyeing automatic controller |
| US20040121456A1 (en) * | 2002-10-31 | 2004-06-24 | Fischer Andrew H. | Rapid cell block embedding method and apparatus |
| US20040266015A1 (en) * | 2002-12-20 | 2004-12-30 | Dakocytomation Denmark A/S | Automated sample processing apparatus and a method of automated treating of samples and use of such apparatus |
| CN1445003A (en) * | 2003-04-18 | 2003-10-01 | 四川大学华西医院 | Method and device for producing bio-derived material |
| JP2009276214A (en) * | 2008-05-15 | 2009-11-26 | Hitachi High-Technologies Corp | Immuno-analyzer |
| CN101294903A (en) * | 2008-06-03 | 2008-10-29 | 武汉大学 | Method for detecting the expression of the same antigen on the same specimen |
| US20120321534A1 (en) * | 2008-06-09 | 2012-12-20 | Shazi Iqbal | Sample processing system and method |
| WO2010024485A1 (en) * | 2008-08-29 | 2010-03-04 | Korea Advanced Institute Of Science And Technology | Apparatus and method for multiple immunoassays on a chip |
| US20140342395A1 (en) * | 2010-04-20 | 2014-11-20 | Eric J. Seibel | Optical projection tomography microscopy (optm) for large specimen sizes |
| CN202141618U (en) * | 2010-10-30 | 2012-02-08 | 湖南省天骑医学新技术有限公司 | Cytological and bacterial staining and preparation machine |
| CN102042924A (en) * | 2010-10-30 | 2011-05-04 | 湖南省天骑医学新技术有限公司 | Dyeing and flaking method for cells or bacteria |
| US20120135458A1 (en) * | 2010-11-30 | 2012-05-31 | General Electric Company | Closed loop monitoring of automated molecular pathology system |
| CN102519775A (en) * | 2011-11-15 | 2012-06-27 | 深圳市科软科技有限公司 | Automatic slide stainer |
| CN104302301A (en) * | 2011-12-07 | 2015-01-21 | 西托维拉公司 | Method and device for sample processing |
| CN202382985U (en) * | 2012-01-11 | 2012-08-15 | 刘振玲 | Urine collection cup with controllable urine discharge structure |
| CN103543058A (en) * | 2012-07-10 | 2014-01-29 | 天津百利鑫生物科技有限公司 | Liquid injection and suction method for cell slide-making dyeing machine and device thereof |
| CN202903569U (en) * | 2012-09-18 | 2013-04-24 | 湖北阳光神琦医用科技有限公司 | Dehydrating and dyeing difunctional machine |
| CN202903571U (en) * | 2012-11-30 | 2013-04-24 | 中国水产科学研究院黑龙江水产研究所 | Gel dyeing disc |
| CN203101148U (en) * | 2013-01-30 | 2013-07-31 | 中南大学 | Staining box for immunohistochemistry |
| CN203132896U (en) * | 2013-04-11 | 2013-08-14 | 王刚平 | Immunohistochemical wet box |
| CN103575584A (en) * | 2013-08-12 | 2014-02-12 | 北京林业大学 | Gel electrophoresis dyeing device |
| CN203414463U (en) * | 2013-08-30 | 2014-01-29 | 海南省交通工程检测中心 | Anti-jamming protection device of dynamic water pressure washout test machine of pavement material |
| CN106716104A (en) * | 2014-09-22 | 2017-05-24 | 株式会社日冷生物科学 | Automatic tissue dyeing apparatus and automatic tissue dyeing method |
| CN204495637U (en) * | 2015-02-07 | 2015-07-22 | 潍坊医学院 | SABC colouration box |
| WO2016196875A1 (en) * | 2015-06-05 | 2016-12-08 | Avanbio Inc. | A component of a device, a device, and a method for purifying and testing biomolecules from biological samples |
| CN204789085U (en) * | 2015-07-20 | 2015-11-18 | 中国人民解放军第三军医大学 | Device is used in immunohistochemical experiment dehydration, washing |
| CN105699156A (en) * | 2016-02-24 | 2016-06-22 | 福州迈新生物技术开发有限公司 | Full-automatic staining instrument for staining tissue samples on glass slides and method for applying full-automatic staining instrument |
| CN105648689A (en) * | 2016-03-21 | 2016-06-08 | 罗湘春 | Wastewater recycling system for dyeing system |
| CN105861310A (en) * | 2016-04-22 | 2016-08-17 | 福州创方医药科技有限公司 | Integral T-cell culture device and use method thereof |
| CN106248461A (en) * | 2016-07-21 | 2016-12-21 | 深圳市美雅洁技术股份有限公司 | A kind of ultrasound wave rapid dewatering system for pathological tissue and using method |
| CN206020152U (en) * | 2016-08-25 | 2017-03-15 | 广州市达诚医疗技术有限公司 | Detection container |
| CN206270135U (en) * | 2016-10-18 | 2017-06-20 | 中国人民解放军第四军医大学 | A kind of new immunohistochemical staining slide apparatus for placing |
| CN206556965U (en) * | 2017-03-10 | 2017-10-13 | 黑龙江省农业科学院作物育种研究所 | A kind of simple dimethylbenzene wash tank device of suitable paraffin section |
Non-Patent Citations (9)
| Title |
|---|
| G.V.CHILDS: "History of Immunohistochemistry", 《PATHOBIOLOGY OF HUMAN DISEASE》 * |
| MONALISAMISHRA: "A quick method to investigate the Drosophila Johnston"s organ by confocal microscopy", 《JOURNAL OF MICROSCOPY AND ULTRASTRUCTURE》 * |
| 丁伟 王德田: "《简明病理学技术》", 28 February 2014 * |
| 侯景辉等: "介绍一种新的免疫组化染色系统", 《临床与实验病理学杂志》 * |
| 戴琦 李冬玲 袁婺洲 吴秀山 李敏: "筛选控制果蝇心脏发育的基因", 《中国动脉硬化杂志》 * |
| 李开霞 马恩波 张建珍 赵一丹 张徐波: "Gal80ts与Gal4组合灵敏控制果蝇中UAS转基因的表达水平", 《昆虫学报》 * |
| 罗玉凤等: "简介全自动免疫组化染色机", 《诊断病理学杂志》 * |
| 赵和: "Bond-Max系统的使用体会及注意事项", 《中华医学会病理学分会2011年全国病理技术新进展研讨会》 * |
| 陈芳等: "全自动免疫组织化学染色技术的应用", 《临床与实验医学杂志》 * |
Cited By (3)
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| US20220204913A1 (en) * | 2019-05-24 | 2022-06-30 | Nanjing GenScript Biotech Co., Ltd. | Biological reaction apparatus and method for performing biological detection on basis of apparatus |
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