CN107810929B - A kind of method for indoor breeding of spiraea aphid for experiment - Google Patents
A kind of method for indoor breeding of spiraea aphid for experiment Download PDFInfo
- Publication number
- CN107810929B CN107810929B CN201711317062.5A CN201711317062A CN107810929B CN 107810929 B CN107810929 B CN 107810929B CN 201711317062 A CN201711317062 A CN 201711317062A CN 107810929 B CN107810929 B CN 107810929B
- Authority
- CN
- China
- Prior art keywords
- ashitaba
- spiraea aphid
- plant
- aphid
- spiraea
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241001124076 Aphididae Species 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 14
- 238000009395 breeding Methods 0.000 title claims abstract description 13
- 230000001488 breeding effect Effects 0.000 title claims abstract description 13
- 238000002474 experimental method Methods 0.000 title claims abstract description 12
- 241000246868 Astilbe japonica Species 0.000 title 1
- 241000196324 Embryophyta Species 0.000 claims abstract description 24
- 241001092387 Spiraea Species 0.000 claims abstract description 16
- 238000005520 cutting process Methods 0.000 claims abstract description 16
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 10
- 239000011159 matrix material Substances 0.000 claims abstract 2
- 229910000838 Al alloy Inorganic materials 0.000 claims description 3
- 230000007613 environmental effect Effects 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 238000011161 development Methods 0.000 abstract description 6
- 241001105098 Angelica keiskei Species 0.000 description 26
- 235000016622 Filipendula ulmaria Nutrition 0.000 description 15
- 241000957095 Spiraea alba Species 0.000 description 13
- 241000273311 Aphis spiraecola Species 0.000 description 9
- 241000238631 Hexapoda Species 0.000 description 5
- 241000220225 Malus Species 0.000 description 5
- 230000018109 developmental process Effects 0.000 description 5
- 241000193981 Loxostege sticticalis Species 0.000 description 4
- 230000000644 propagated effect Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 241001072282 Limnanthes Species 0.000 description 3
- 241001481296 Malus spectabilis Species 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 206010059866 Drug resistance Diseases 0.000 description 2
- 244000308505 Filipendula ulmaria Species 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 241001600407 Aphis <genus> Species 0.000 description 1
- 241000258937 Hemiptera Species 0.000 description 1
- 241000220324 Pyrus Species 0.000 description 1
- 241001106462 Ulmus Species 0.000 description 1
- 244000069780 Youngia japonica Species 0.000 description 1
- 235000021016 apples Nutrition 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000011437 continuous method Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 235000021017 pears Nutrition 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/30—Rearing or breeding invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cultivation Of Plants (AREA)
Abstract
本发明公开了一种室内饲养实验用绣线菊蚜的方法，包括如下步骤：取栽培的一年生、生长健壮的明日叶枝条，剪成长10‑15cm、带2‑5片叶片剪口倾斜的插条，扦插于育苗基质中进行培育，待其嫩芽长至3±0.5cm时，在野外果树植物上采集的绣线菊蚜带回室内，移入一部分所述的明日叶植株上；待另一部分的明日叶植株扦插成活后60d，将植株移入育苗盆内，置于20‑30℃的环境中培育；待明日叶长满绣线菊蚜时，将带有蚜虫的明日叶叶片剪下，移入所得的明日叶植株上进行饲养即可。本发明采用明日叶苗饲养、扩繁实验用绣线菊蚜，能够很好地满足实验用绣线菊蚜的生长发育，大量繁殖的需求，同时降低了饲养成本。The invention discloses a method for indoor breeding of Spiraea aphid for experiments. strips, the cuttings are cultivated in the seedling matrix, and when their tender buds grow to 3±0.5cm, the Spiraea aphid collected on the wild fruit tree plants is brought back indoors, and moved into a part of the described Ashitaba plants; 60 days after the cuttings of the ashitaba plants survived, the plants were moved into seedling pots and cultivated in an environment of 20-30 °C; What is necessary is just to raise on the obtained ashitaba plants. The present invention adopts the Spiraea aphid for breeding and propagation experiments of Astoria leaf seedlings, which can well meet the needs of the growth, development and mass reproduction of the experimental Spiraea aphid, and simultaneously reduces the feeding cost.
Description
Technical Field
The invention relates to the field of insect breeding, in particular to a method for feeding experimental meadowsweet aphids indoors.
Background
Aphis citricola Van der Goot belonging to Hemiptera of Aphis family, Aphididae, also known as Aphididae, is distributed abroad in Japan, Korea, North America and China, is widely distributed in various large apple production areas in China, and is a foliar pest with serious harm. The main hosts of the meadowsweet comprise various rosaceous fruit trees such as apples, pears, Chinese flowering crabapples and the like, and plants such as meadowsweet, elm leaves and the like. The insect can be generated along with the growth period of the tender tips of the fruit trees, and the piercing and sucking of fruit tree juice causes the downward bending or transverse rolling of leaves, so that the tree vigor is weakened, the photosynthesis is influenced, various diseases are induced at the same time, the growth of the fruit trees and the quality of fruits are seriously influenced, and the serious economic loss is caused to the production of the fruit trees.
The experimental meadow aphids are mostly collected temporarily in the field, and because the pesticide used in the field is unknown and the natural resistance of meadow aphid populations in different areas is greatly different, the experimental effect of the later period is greatly influenced by the meadow aphids collected from different areas. The Aphis citricola population bred by indoor breeding has higher sensitivity to experimental agents, better bioassay effect and real and reliable result.
With the continuous deepening of entomology research and the continuous development of new pest control technologies, a large number of experimental insects with unified physiological standards for experiment supply are the basis for well-done research. Research work on indoor feeding of various insects has become one of the basic techniques for research in entomology and new techniques for pest control. However, at present, the indoor breeding of the meadowsweet mainly depends on the field hosts such as apple seedlings, Chinese flowering apple seedlings and the like. The woody plants are not easy to survive in indoor cultivation, and the economic and management costs are high; the growth and development are extremely slow, and the growth speed of the tender tips can not meet the requirement of expanding propagation of the Aphis citricola completely; and the problems of falling leaves in autumn and winter and the like exist, and the food can not be continuously provided; these drawbacks prevent the indoor preservation and propagation of the aphid population. Therefore, there is a need to find a simpler, less expensive and continuous method for breeding the aphid meadowfoam.
Disclosure of Invention
In order to solve the problems, the invention provides a method for feeding experimental meadowsweet indoors, which can be operated continuously, is simple and convenient to operate and has low cost.
In order to achieve the purpose, the invention adopts the technical scheme that:
a method for feeding experimental meadowsweet aphids indoors is used for feeding experimental meadowsweet aphid populations through cultured angelica keiskei and specifically comprises the following steps:
s1 cutting angelica keiskei koidzumi
Cutting the annual strong branches of the angelica keiskei with length of 10-15cm and inclined cut openings with 2-5 leaves; cutting the cutting in a seedling raising substrate, placing the seedling raising substrate in an environment with the temperature of 20-30 ℃ for cultivation, keeping the humidity at 75%, and illuminating at 16L: 8D;
s2 cultivation of Angelica keiskei
And (3) when the angelica keiskei plants in the step S1 are planted and survived for 60 days and the angelica keiskei plants are 20-25cm high, transferring the plants into a seedling pot, placing the seedling pot in an environment with the temperature of 20-30 ℃ for cultivation, keeping the humidity at 75%, and illuminating at 16L: 8D;
s3, returning the meadow moth collected on the wild fruit tree plant to the room, and transferring the meadow moth to the angelica keiskei plant with the tender shoot length of 3 +/-0.5 cm obtained in the step S1;
s4, when the leaf of the angelica keiskei in the step S3 is full of the aphid of the spiraea littoralis, cutting off the leaf of the angelica keiskei with the aphid, transferring the leaf of the angelica keiskei to the plant of the angelica keiskei obtained in the step S2, and transferring 200 +/-5 heads of the aphid of the spiraea littoralis to each plant.
Preferably, the seedling raising container in the step S1 adopts a plastic flowerpot with a diameter of 10 cm; the nursery pot in the step S2 is a plastic flowerpot with the diameter of 30 cm.
Preferably, the preservation and propagation of the experimental Aphis citricola are carried out in an insect cage with an aluminum alloy frame with the length of 40cm, the width of 40cm, the height of 50cm, a stainless steel gauze with 120 meshes and a glass bottom;
preferably, the environmental conditions for feeding the meadowsweet are that the temperature is 25 +/-2 ℃, the relative humidity is 75 +/-5%, the illumination is 16L: and 8D.
The invention has the following beneficial effects:
according to the method, the Aphis citricola for the experiment is propagated by using the angelica keiskei seedling, so that the risk of collecting the Aphis citricola from the field is reduced, and the real effect of the experiment is greatly improved. The invention adopts the self-cultured angelica keiskei seedling to realize the autonomous culture of the meadowfoam aphid, thereby avoiding the influence and experimental error on some experiments of drug resistance detection and drug resistance screening. In addition, the invention overcomes the problems that woody plants such as apple seedlings, Chinese flowering apple seedlings and the like are not easy to survive in indoor cultivation, extremely slow in growth and development, fallen leaves in autumn and winter and the like, and solves the problem that the meadowsweet aphid population can not be stored and propagated continuously indoors for many years.
In conclusion, the experimental meadowsweet is creatively bred and propagated by the angelica keiskei seedling, the requirements of growth, development and mass propagation of the experimental meadowsweet can be well met, and meanwhile the breeding cost is reduced. In addition, the Aphis citricola is bred and propagated in a bright day leaf seedling culture room, the bright day leaf seedling is cultured in a special seedling culture room, the growth and development of the bright day leaf seedling culture room are not influenced by seasons and the external environment, and sufficient bred host plants are provided for breeding and propagating the Aphis citricola on a large scale.
Detailed Description
In order that the objects and advantages of the invention will be more clearly understood, the invention is further described in detail below with reference to examples. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The Angelica Keiskei (Angelica Keiskei Koidzmi), also called longevity vegetable and crepis japonica, used in the following examples is a perennial herb, is native to japan, and is currently cultivated in many places in China. The experimental Aphis citricola is cultured in an aluminum alloy frame with the length of 40cm, the width of 40cm and the height of 50cm, a 120-mesh stainless steel gauze and an insect-raising cage with a glass bottom.
Examples
A method for feeding experimental meadowsweet indoors comprises the following steps:
s1 cutting angelica keiskei koidzumi
Cutting the annual strong branches of the angelica keiskei with length of 10-15cm and inclined cut openings with 2-5 leaves; cutting the cutting in a seedling raising substrate, placing the seedling raising substrate in an environment with the temperature of 20-30 ℃ for cultivation, keeping the humidity at 75%, and illuminating at 16L: 8D; the adopted seedling raising container adopts a plastic flowerpot with the diameter of 10 cm;
s2 cultivation of Angelica keiskei
And (3) when the angelica keiskei plants in the step S1 are planted and survived for 60 days and the angelica keiskei plants are 20-25cm high, transferring the plants into a seedling pot, placing the seedling pot in an environment with the temperature of 20-30 ℃ for cultivation, keeping the humidity at 75%, and illuminating at 16L: 8D; the adopted seedling raising pot is a plastic flowerpot with the diameter of 30 cm; the number of the seedling raising pots is 3 times that of the seedling raising containers in the step S1, and the design can ensure that the apple yellow aphids after propagation have enough food;
s3, returning the meadow moth collected on the wild fruit tree plant to the room, and transferring the meadow moth to the angelica keiskei plant with the tender shoot length of 3 +/-0.5 cm obtained in the step S1;
s4, when the leaf of the angelica keiskei in the step S3 is full of the aphid of the spiraea ulmaria, cutting off the leaf of the angelica keiskei with the aphid, transferring the leaf of the angelica keiskei to the plant of the angelica keiskei obtained in the step S2, and transferring 200 +/-5 heads of the aphid of the spiraea ulmaria to each plant; the environmental conditions for feeding the meadowfoam aphids are that the temperature is 25 +/-2 ℃, the relative humidity is 75 +/-5%, the illumination is 16L: and 8D.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and these improvements and modifications should also be construed as the protection scope of the present invention.
Claims (4)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711317062.5A CN107810929B (en) | 2017-11-25 | 2017-11-25 | A kind of method for indoor breeding of spiraea aphid for experiment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711317062.5A CN107810929B (en) | 2017-11-25 | 2017-11-25 | A kind of method for indoor breeding of spiraea aphid for experiment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107810929A CN107810929A (en) | 2018-03-20 |
| CN107810929B true CN107810929B (en) | 2020-04-03 |
Family
ID=61606798
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711317062.5A Expired - Fee Related CN107810929B (en) | 2017-11-25 | 2017-11-25 | A kind of method for indoor breeding of spiraea aphid for experiment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107810929B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105557643A (en) * | 2016-01-25 | 2016-05-11 | 北京市农林科学院 | Method for artificially propagating mass experimental green peach aphids |
-
2017
- 2017-11-25 CN CN201711317062.5A patent/CN107810929B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105557643A (en) * | 2016-01-25 | 2016-05-11 | 北京市农林科学院 | Method for artificially propagating mass experimental green peach aphids |
Non-Patent Citations (2)
| Title |
|---|
| 一种扩繁烟蚜茧蜂的载体植物适合性评价;黄凤霞等;《环境昆虫学报》;20150725;第37卷(第4期);第71-75页 * |
| 不同温度对绣线菊蚜(Aphis citricola Van der Goot)实验种群的影响(英文);王守宝等;《西北农业学报》;20080916;第17卷(第5期);第827-833页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107810929A (en) | 2018-03-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105191763A (en) | Vegetable organic soil-less culture method, culture medium and manufacturing method thereof | |
| CN110521674B (en) | Indoor breeding method for Brachypodium banseri | |
| CN108157180B (en) | Open type factory rapid propagation method for potato virus-free seedlings | |
| CN102845313A (en) | Method for quickly in-vitro actinidia kolomikta propagating | |
| CN103875386A (en) | Soilless seedling cutting raising method of gardenia jasminoides in potted trays of rice field | |
| CN103535328B (en) | Method for industrially breeding phyoseiulus persimilis | |
| CN106689066A (en) | Method for quickly breeding aphidius gifuensis | |
| CN104041412A (en) | Rapid propagation method for tissue culture of Guizhou hemiboea cavaleriei | |
| CN104855292B (en) | A kind of method of Cinnamomum kanahirai hay stem segment tissue culture fast breeding | |
| CN101167441B (en) | Method for Tissue Culture and Rapid Propagation of S. unifolia clone | |
| CN105165726B (en) | A method for three-dimensionally propagating M. aphids by using cutting plants | |
| CN107810929B (en) | A kind of method for indoor breeding of spiraea aphid for experiment | |
| Chindi et al. | Enhancing potato seed production using rapid multiplication techniques. | |
| CN103404319A (en) | Tilia henryana sowing and seedling raising method | |
| KR101281934B1 (en) | Nado Lantern New breed Prince | |
| CN101429489A (en) | Cinnamomum kanahirai hay body embryo culture medium and tissue culture rapid propagation method | |
| CN112825817B (en) | The method of indoor rearing and breeding of nymphalus under full artificial condition | |
| CN106106377A (en) | A kind of method of indoor feeding Fructus Foeniculi thin wing open country snout moth's larva larva | |
| Takeda et al. | Method for producing long-cane blackberry plants | |
| CN103168686B (en) | Rapid propagation method for tissue culture of aspidistra minutiflora | |
| CN117694152B (en) | Trichogramma releasing method with soybean seedlings as carrier and soybean aphid honeydew as nutrition supplementing function | |
| CN108353855B (en) | Artificial breeding method of cyrtorhinus squarrosus | |
| CN108513953B (en) | Method for improving hidden seed parasitism rate of parasitic wasps on bemisia tabaci MED | |
| Vince-Prue et al. | Propagating plants vegetatively | |
| CN107960323A (en) | A kind of mating system of buerretiodendron hsienmu tissue-cultured seedling |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200403 |