A kind of vegetable protein beverage and preparation method thereof
Technical field
The present invention relates to a kind of vegetable protein beverage and preparation method thereof.
Background technology
Vegetable protein beverage is for primary raw material (such as soybean, peanut, almond, walnut kernel, coconut palm with plant kernel, pulp etc.
Son, ear of maize), the processed manufactured emulsion liquid drink based on vegetable protein.Vegetable protein beverage be free of or only contain compared with
Few cholesterol, rich in protein, amino acid and appropriate unrighted acid, nutritional ingredient is more complete, therefore deep by consumer
Welcome.In recent years, vegetable protein beverage started to show up prominently on the beverage market in China, and its main cause is with life
Horizontal continuous improvement and the continuous enhancing of health perception, consumer's soda to quench one's thirst of being relieved summer heat from simple satisfaction progressively turn
To nutrition, health care, the requirement of function, this is also that soft drink development is inevitable.At present, the vegetable protein beverage in China every year with
30% speed increases, it may be said that vegetable protein beverage is in a development best period, and the is introduced from Guangdong at the beginning of the eighties
So far, the country has thousands of soymilk processing factories to one soy milk production line.And China's plant protein resource is from north to the South Pole
Abundant, by taking soybean as an example, China is one of soybean producing country of the world four, thus development vegetable protein beverage be suitable for China,
Improve a supplement measure of national protein intake.
Chinese patent application CN201610084572.1 discloses a kind of lactobacillus-fermented vegetable protein beverage and its preparation
Method, described lactobacillus-fermented vegetable protein milk beverage is by comprising the following steps method:Including step 1, prepare fermentation base-material;
Step 2, lactobacillus-fermented vegetable protein beverage is prepared, i.e., diluted base-material described in step 1, add sucrose, stabilizer, tart flavour
Agent, homogeneous obtains lactobacillus-fermented vegetable protein beverage after essence acid adjustment.The invention is although nutritious, but effect is more single
One, and stability is bad.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of vegetable protein beverage, and it not only has abundant nutriture value
Value, also there is preferable anti-oxidant, antibiotic effect and stability.
In order to solve the above technical problems, the technical scheme is that:
A kind of vegetable protein beverage, it is made up of following components in percentage by weight:Soybean protein 15-20%, cabbage
Protein 10-15%, stabilizer 0.2-0.5%, sugared 5-6%, emulsifying agent 0.1-0.4%, thickener 0.3-0.6%, remaining is water.
Further, the preparation process of the cabbage albumen is:
1) it is placed in after cabbage is cleaned in baking oven and constant weight is dried at 60 DEG C, crushed after taking-up, is obtained after crossing 60 mesh sieves
Cabbage dry powder;
2) cabbage dry powder is added in petroleum ether, flows back 6 hours after being heated to reflux temperature, be placed in filter residue after filtering
In baking oven, constant weight is dried at 60 DEG C and obtains pre-processing cabbage dry powder;
3) pretreatment cabbage dry powder adding in sodium hydroxide solution, constant temperature extracts 4 hours and obtains extract solution at 60 DEG C,
Extract solution is filtrated to get filtrate, filtrate is sunk with being centrifuged 15 minutes under 5000rpm rotating speeds after salt acid for adjusting pH value to 5.0
Starch, sediment is freeze-dried to constant weight and obtains cabbage crude protein;
4) by cabbage crude protein successively with 8000 molecular weight and the ultrafiltration membrance filter of 12000 molecular weight, freeze-drying is extremely
Cabbage albumen is obtained after constant weight.
Further, in the step 2), the weight ratio of cabbage dry powder and petroleum ether is 1:1.
Further, in the step 3), the mass fraction of sodium hydroxide solution is 3%, pretreatment cabbage dry powder with
The weight ratio of sodium hydroxide solution is 1:22.
Further, the stabilizer by etc. weight sodium carboxymethylcellulose and propylene glycol alginate mix.
Further, the sugar is white granulated sugar.
Further, the emulsifying agent is sucrose fatty ester.
Further, the thickener is carragheen.
Further, the water is pure water.
Another technical problem to be solved by the present invention is that provide the preparation method of above-mentioned vegetable protein beverage.
In order to solve the above technical problems, technical scheme is:
A kind of preparation method of vegetable protein beverage, comprises the following steps:
Weigh each component by weight percentage, by sugar with its etc. weight water dissolving after be filtrated to get liquid glucose, by liquid glucose
Mixed with other components and remaining water, the homogenizer homogenization of 25MPa pressure is used after being stirred at 60 DEG C, after taking-up
Handled 10 seconds with board-like 100 DEG C of sterilizer, Sterile vacuum is filling when being cooled to 10 DEG C obtains vegetable protein beverage.
Compared with prior art, the invention has the advantages that:
(1) soybean protein is a kind of vegetable protein, and its amino acid composition is close with milk protein, except methionine omits
Low outer, remaining essential amino acids content is relatively abundant, can be equal on nutritive value with animal protein, on gene structure also most
It is the phytoprotein of most nutrition close to human amino acid, the present invention is that key component has the battalion enriched using soybean protein
Support value.
(2) cabbage contains abundant leaf protein, and the present invention passes through cabbage successively to be cleaned, dries, crushing, using oil
Extract to obtain crude protein by sodium hydroxide after ether pretreatment, then with milipore filter carry out Purification by filtration handle to obtain purity it is higher
Cabbage albumen, it not only contains very high nutritive value, also has very strong antioxidation activity and bacteriostatic activity, therefore can be big
Width improves the anti-oxidation efficacy and antibiotic effect of vegetable protein beverage.
(3) sodium carboxymethylcellulose and propylene glycol alginate are respectively provided with stronger stability, the two and use can produce collaboration
Effect, so as to greatly improve the stability of vegetable protein beverage;In addition, propylene glycol alginate can also effectively improve soybean protein and
The dispersiveness of cabbage albumen, so as to further improve the anti-oxidation efficacy and antibiotic effect of vegetable protein beverage.
Embodiment
The present invention is described in detail below in conjunction with specific embodiment, herein schematic description and description of the invention
For explaining the present invention, but it is not as a limitation of the invention.
Embodiment 1-4
Vegetable protein beverage is prepared according to the ratio of table 1 (by weight percentage):
Table 1
Embodiment 1-4 preparation method comprises the following steps:
1) it is placed in after cabbage is cleaned in baking oven and constant weight is dried at 60 DEG C, crushed after taking-up, is obtained after crossing 60 mesh sieves
Cabbage dry powder;
2) cabbage dry powder is added in petroleum ether, the weight ratio of cabbage dry powder and petroleum ether is 1:1, it is heated to flowing back
Flow back 6 hours after temperature, filter residue is placed in baking oven after filtering, constant weight is dried at 60 DEG C and obtains pre-processing cabbage dry powder;
3) cabbage dry powder will be pre-processed to add in the sodium hydroxide solution that mass fraction is 3%, pretreatment cabbage does
The weight of powder and sodium hydroxide solution ratio is 1:Constant temperature is extracted 4 hours and obtains extract solution at 22,60 DEG C, and extract solution is filtrated to get
Filtrate, filtrate is obtained into sediment with being centrifuged 15 minutes under 5000rpm rotating speeds after salt acid for adjusting pH value to 5.0, sediment is cold
It is lyophilized dry to obtain cabbage crude protein to constant weight;
4) by cabbage crude protein successively with 8000 molecular weight and the ultrafiltration membrance filter of 12000 molecular weight, freeze-drying is extremely
Cabbage albumen is obtained after constant weight.
5) weigh each component by weight percentage, by white granulated sugar with its etc. weight dissolved in purified water after be filtrated to get sugar
Liquid, liquid glucose is mixed with other components and remaining pure water, it is equal with the homogenizer of 25MPa pressure after being stirred at 60 DEG C
Matter processing, handled 10 seconds with board-like 100 DEG C of sterilizer after taking-up, Sterile vacuum is filling when being cooled to 10 DEG C obtains vegetable protein drink
Material.
Comparative Examples 1
Do not include cabbage albumen in component, other components and preparation method are same as Example 4.
Comparative Examples 2
Do not include sodium carboxymethylcellulose in component, other components and preparation method are same as Example 4.
Comparative Examples 3
Do not include propylene glycol alginate in component, other components and preparation method are same as Example 4.
Comparative example
Comparative example is the embodiment 1 of Application No. CN201610084572.1 Chinese patent.
Experimental example one:Anti-oxidation efficacy is tested
Determine the TAC of each vegetable protein beverage by FRAP methods, TAC is higher show it is anti-oxidant
Effect is better, and test result is as shown in table 2:
| |
TAC (mmolTrolox/100mL) |
| Embodiment 1 |
0.1389 |
| Embodiment 2 |
0.1383 |
| Embodiment 3 |
0.1370 |
| Embodiment 4 |
0.1402 |
| Comparative Examples 1 |
0.1044 |
| Comparative Examples 2 |
0.1393 |
| Comparative Examples 3 |
0.1217 |
| Comparative example |
0.1008 |
Table 2
As seen from Table 2, it is many to be above comparative example for 1-4 of embodiment of the present invention TAC, shows the present invention
It is best with preferable anti-oxidation efficacy, the anti-oxidation efficacy of wherein embodiment 4.Comparative Examples 1-3 constituent part and reality
It is different to apply example 4, wherein, the TAC of Comparative Examples 1 is greatly reduced, and it is to improve anti-oxidant work(to illustrate cabbage albumen
The principal element of effect;The TAC of Comparative Examples 2 maintains an equal level with embodiment 1-4, illustrates that sodium carboxymethylcellulose resists
Oxidation efficiency does not influence substantially;The TAC of Comparative Examples 3 slightly reduces, and illustrates that propylene glycol alginate can be effective
Improve the dispersiveness of cabbage albumen.
Experimental example two:Antibiotic effect is tested
Each vegetable protein beverage is tested respectively to common microbiological-saccharomycete, staphylococcus aureus, black song in food
Mould, mould bacteriostasis rate, bacteriostasis rate is higher to show that antibiotic effect is stronger, and test result is as shown in table 3:
Table 3
As seen from Table 3,1-4 of the embodiment of the present invention to saccharomycete, staphylococcus aureus, aspergillus niger, mould it is antibacterial
It is many that rate is above comparative example, shows that the present invention has a preferable antibiotic effect, the antibiotic effect of wherein embodiment 4 is best.Ginseng
Constituent part than embodiment 1-3 is different from embodiment 4, wherein, the bacteriostasis rate of Comparative Examples 1 is greatly reduced, and illustrates to roll up the heart
Dish albumen is the principal element for improving antibiotic effect;The bacteriostasis rate of Comparative Examples 2 maintains an equal level with embodiment 1-4, illustrates carboxymethyl
Sodium cellulosate does not influence substantially on antibiotic effect;The bacteriostasis rate of Comparative Examples 3 has small size reduction, illustrates propylene glycol alginate
Effectively improve the dispersiveness of cabbage albumen.
Experimental example three:Stability test
By centrifuge tube drying to constant weight, moved to after each vegetable protein beverage is weighed into 35g respectively in centrifuge tube, 4000rpm
Centrifuged 30 minutes under rotating speed, remove upper strata oil reservoir and clear liquid, the centrifuge tube containing precipitation is inverted, weighs, calculates after 3 hours
Centrifugation rate, centrifugation rate=(centrifuge tube weight before centrifuge tube weight-test after test)/35g × 100%, centrifugation are heavy
Shallow lake rate is lower to show that stability is better, and test result is as shown in table 4:
| |
Centrifugation rate (%) |
| Embodiment 1 |
0.33 |
| Embodiment 2 |
0.35 |
| Embodiment 3 |
0.36 |
| Embodiment 4 |
0.31 |
| Comparative Examples 1 |
0.34 |
| Comparative Examples 2 |
0.95 |
| Comparative Examples 3 |
0.98 |
| Comparative example |
2.44 |
Table 4
As seen from Table 4, it is many to be below comparative example for 1-4 of embodiment of the present invention centrifugation rate, shows tool of the present invention
There is preferable stability.Comparative Examples 1-3 constituent part is different from embodiment 4, wherein, the centrifugation of Comparative Examples 1 is sunk
Shallow lake rate maintains an equal level with embodiment 1-4, illustrates that cabbage albumen does not influence substantially on stability;The centrifugation of Comparative Examples 2,3 is sunk
Shallow lake rate has to be risen by a small margin, illustrates that sodium carboxymethylcellulose and propylene glycol alginate can effectively improve stability.
The above-described embodiments merely illustrate the principles and effects of the present invention, not for the limitation present invention.It is any ripe
Know the personage of this technology all can carry out modifications and changes under the spirit and scope without prejudice to the present invention to above-described embodiment.Cause
This, those of ordinary skill in the art is complete without departing from disclosed spirit and institute under technological thought such as
Into all equivalent modifications or change, should by the present invention claim be covered.