CN107303303B - Prepare Tongkat Ali active ingredient and combination application - Google Patents
Prepare Tongkat Ali active ingredient and combination application Download PDFInfo
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- CN107303303B CN107303303B CN201610250227.0A CN201610250227A CN107303303B CN 107303303 B CN107303303 B CN 107303303B CN 201610250227 A CN201610250227 A CN 201610250227A CN 107303303 B CN107303303 B CN 107303303B
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- 239000004480 active ingredient Substances 0.000 title abstract description 10
- 239000011347 resin Substances 0.000 claims abstract description 18
- 229920005989 resin Polymers 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 17
- 239000012528 membrane Substances 0.000 claims abstract description 15
- 230000002708 enhancing effect Effects 0.000 claims abstract description 14
- 239000006286 aqueous extract Substances 0.000 claims abstract description 12
- 238000001179 sorption measurement Methods 0.000 claims abstract description 11
- 230000036299 sexual function Effects 0.000 claims abstract description 10
- 238000002137 ultrasound extraction Methods 0.000 claims abstract description 10
- 239000007864 aqueous solution Substances 0.000 claims abstract description 7
- 230000036039 immunity Effects 0.000 claims abstract description 6
- 201000005569 Gout Diseases 0.000 claims abstract description 5
- 238000010828 elution Methods 0.000 claims abstract description 5
- 238000000926 separation method Methods 0.000 claims abstract description 5
- 238000002360 preparation method Methods 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- DQJCDTNMLBYVAY-ZXXIYAEKSA-N (2S,5R,10R,13R)-16-{[(2R,3S,4R,5R)-3-{[(2S,3R,4R,5S,6R)-3-acetamido-4,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}-5-(ethylamino)-6-hydroxy-2-(hydroxymethyl)oxan-4-yl]oxy}-5-(4-aminobutyl)-10-carbamoyl-2,13-dimethyl-4,7,12,15-tetraoxo-3,6,11,14-tetraazaheptadecan-1-oic acid Chemical compound NCCCC[C@H](C(=O)N[C@@H](C)C(O)=O)NC(=O)CC[C@H](C(N)=O)NC(=O)[C@@H](C)NC(=O)C(C)O[C@@H]1[C@@H](NCC)C(O)O[C@H](CO)[C@H]1O[C@H]1[C@H](NC(C)=O)[C@@H](O)[C@H](O)[C@@H](CO)O1 DQJCDTNMLBYVAY-ZXXIYAEKSA-N 0.000 claims description 7
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- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 6
- 150000001413 amino acids Chemical class 0.000 claims description 6
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- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- 238000001035 drying Methods 0.000 claims 1
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- 238000001223 reverse osmosis Methods 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 230000002929 anti-fatigue Effects 0.000 abstract description 8
- 239000003480 eluent Substances 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 239000000470 constituent Substances 0.000 abstract description 3
- 235000017807 phytochemicals Nutrition 0.000 abstract description 2
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- UCUWZJWAQQRCOR-UHFFFAOYSA-N Pasakbumin A Natural products O1C(=O)C(O)C2(O)C(=C)C(O)C3(O)C4C5(C)C(O)C(=O)C=C(C)C5CC1C42CO3 UCUWZJWAQQRCOR-UHFFFAOYSA-N 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- UCUWZJWAQQRCOR-OKNZMGBLSA-N eurycomanone Chemical compound O1C(=O)[C@H](O)[C@@]2(O)C(=C)[C@@H](O)[C@@]3(O)[C@@H]4[C@@]5(C)[C@H](O)C(=O)C=C(C)[C@@H]5C[C@@H]1[C@]42CO3 UCUWZJWAQQRCOR-OKNZMGBLSA-N 0.000 description 4
- 239000009947 eurycomanone Substances 0.000 description 4
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- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 240000005739 Eurycoma longifolia Species 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- ZUOUZKKEUPVFJK-UHFFFAOYSA-N diphenyl Chemical compound C1=CC=CC=C1C1=CC=CC=C1 ZUOUZKKEUPVFJK-UHFFFAOYSA-N 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 2
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- SNGWXAVWKXBHJD-UHFFFAOYSA-N Eurycolactone Natural products CC1C(O)C(O)C2C3(C)C(O)C(O)C=C(C)C3C(OC(=O)C)C4OC(=O)C1C24C SNGWXAVWKXBHJD-UHFFFAOYSA-N 0.000 description 1
- OGHYZHNTIINXEO-MGBQOKOWSA-N Eurycomalactone Chemical compound O[C@@H]([C@]1(C)[C@H]2[C@H]3O)C(=O)C=C(C)[C@@H]1CC(=O)[C@@]2(C)[C@H]1C(=O)O[C@@H]3[C@@H]1C OGHYZHNTIINXEO-MGBQOKOWSA-N 0.000 description 1
- OGHYZHNTIINXEO-ONPJAVPJSA-N Eurycomalactone Natural products O=C1O[C@H]2[C@H](O)[C@@H]3[C@@]4(C)[C@H](O)C(=O)C=C(C)[C@@H]4CC(=O)[C@]3(C)[C@H]1[C@H]2C OGHYZHNTIINXEO-ONPJAVPJSA-N 0.000 description 1
- TYXFVKGQQWDJKA-SREAOFIKSA-N Eurycomaoside Natural products O([C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1)[C@H]1[C@]2(C)[C@@H]3[C@@H](O)[C@H](O)[C@H](C)[C@@H]4C(=O)O[C@H]([C@H](O)[C@H]2[C@](O)(C)C=C1)[C@]34C TYXFVKGQQWDJKA-SREAOFIKSA-N 0.000 description 1
- LZKVXMYVBSNXER-YZPKDWIXSA-N Glaucarubin Chemical compound O[C@@H]([C@]1(C)[C@@H]23)[C@@H](O)C=C(C)[C@@H]1C[C@@H]1[C@@]43CO[C@@]2(O)[C@H](O)[C@H](C)[C@@H]4[C@@H](OC(=O)[C@@](C)(O)CC)C(=O)O1 LZKVXMYVBSNXER-YZPKDWIXSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- 241001529246 Platymiscium Species 0.000 description 1
- 241000251778 Squalus acanthias Species 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000007059 acute toxicity Effects 0.000 description 1
- 231100000403 acute toxicity Toxicity 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000000078 anti-malarial effect Effects 0.000 description 1
- 239000003430 antimalarial agent Substances 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 235000010290 biphenyl Nutrition 0.000 description 1
- 239000004305 biphenyl Substances 0.000 description 1
- ZERVJPYNQLONEK-UHFFFAOYSA-N canthin-6-one Chemical class C12=CC=CC=C2N2C(=O)C=CC3=NC=CC1=C32 ZERVJPYNQLONEK-UHFFFAOYSA-N 0.000 description 1
- MOCOVNGOINOTNW-FDPUJYPZSA-N chembl1171669 Chemical compound C([C@@]12[C@]3(O)[C@@H](O)C(=O)O[C@H]4[C@]53CO[C@@]([C@@H]2O)(O)[C@@H]5[C@@]2(C)[C@H](O)C(=O)C=C([C@@H]2C4)C)O1 MOCOVNGOINOTNW-FDPUJYPZSA-N 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000012043 crude product Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 229930004069 diterpene Natural products 0.000 description 1
- 125000000567 diterpene group Chemical group 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
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- 231100000404 nontoxic agent Toxicity 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- MOCOVNGOINOTNW-UHFFFAOYSA-N pasakbumin-B Natural products C1C2C(C)=CC(=O)C(O)C2(C)C2C(C3O)(O)OCC22C1OC(=O)C(O)C2(O)C13CO1 MOCOVNGOINOTNW-UHFFFAOYSA-N 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
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- 238000010992 reflux Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- WJCNZQLZVWNLKY-UHFFFAOYSA-N thiabendazole Chemical compound S1C=NC(C=2NC3=CC=CC=C3N=2)=C1 WJCNZQLZVWNLKY-UHFFFAOYSA-N 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
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- 238000012549 training Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention belongs to phytochemicals production to extract field, and in particular to the brand-new method for preparing Tongkat Ali active ingredient and combination application.Specially：Tongkat Ali is crushed at a certain temperature obtains aqueous extract with aqueous solution adverse current ultrasonic extraction；Aqueous extract macroporous resin adsorption, binding component use eluent gradient elution, collect target component, and effective constituents A is made；Target components are collected, active ingredient B is made using filter membrane classification separation in macroreticular resin outflow part；Effective constituents A and B are mixed according to a certain percentage, and combination of active principles is made.This method is separated using temperature controlled ultrasonic extraction with aqueous solution and with macroporous resin adsorption and UF membrane, remain the bioactivity of active ingredient and be selectively enriched with its ground, final rational allocation, which is made, brand-new to be had enhancing sexual function, improve fertility-rate, antifatigue, strengthen immunity of refreshing oneself, increase physical efficiency, the Tongkat Ali combination of active principles for alleviating gout.
Description
Technical field
The invention belongs to phytochemicals production to extract field, and in particular to prepares Tongkat Ali effective ingredients in plant and combination
The method of application.
Background introduction
Tongkat Ali (Eurycoma longifolia) is Simarubaceae Tongkat Ali platymiscium, and wild bush, is grown on red
Near road in original tropical rain forest humidity sandy soil, the countries in Southeast Asia such as Malaysia, Indonesia, Vietnam distribution compared with
Extensively.Modern research shows that Tongkat Ali has very high medical value.Tongkat Ali plant is obtained according to various Different treatments
The component obtained has active anticancer respectively, and antimalarial active, improves the effect such as sexual function.In addition, its hypoglycemic, antibacterial,
Treatment gout, osteoporosis etc. also have certain effect.The chemical composition separated from Tongkat Ali different parts
Based on the Diterpenes of the skeleton containing guassin and canthinone Alkaloid, such as eurycomaoside, eurycolactone,
Eurycomalactone, eurycomanone (eurycomanone) and pasakbumin-B.In addition, also containing biphenyl lignin, spiny dogfish
Ene derivative, active polysaccharide, glycopeptide and a variety of amino acid.At present, found by our research, the Tongkat Ali that Indonesia introduces
The combination of active principles obtained using this method extraction is had enhancing sexual function, improves fertility-rate, refreshes oneself that antifatigue, enhancing is immune
Power, increase physical efficiency, alleviate pain wind action.
The situation that Tongkat Ali plant uses directly as medicine materical crude slice is more universal, but Tongkat Ali brews that there are mouthfeel is bitter
The problem of puckery, drug action low aspect such as low with utilization ratio of drug.Therefore, it is high to study Tongkat Ali active constituent content, prepares
Process, medicinal effects are clear and definite, the product of good mouthfeel just becomes particularly significant.Research data shows that Tongkat Ali extracts
Mainly using solvent refluxing extraction or high-temperature water extraction process, if patent CN104757424A is using decocting method extraction；Patent
US20070224300A1 uses extracting in boiling water；" Tongkat Ali is to training rat TESTO content, metabolism and anti-movement
The influence of fatigue capability " article is prepared using boiling water；" separation and identification of Tongkat Ali chemical composition " article is flowed back using water
Extraction.Patent CN103408564A extracts Tongkat Ali with organic solvent；" the experiment of Eurycoma longifolia extract product antifatigue effect
Research " article uses alcohol extracting taking technique.Although both the above mode extraction product amount is relatively more, extracting in boiling water and organic
Solvent extraction mode can make a variety of active ingredients among plant decompose or be denatured, and reduce drug effectiveness, while the energy
Consumption is more, and environmentally friendly difficulty is high, and solvent cost is big, and extraction process harmfulness is strong.
Many results of study show, Tongkat Ali plant have really obvious enhancing sexual function, improve fertility-rate,
Refresh oneself antifatigue, strengthen immunity, enhancing physical efficiency, alleviate pain wind action, the extract obtained in a variety of different ways is universal
With certain effect of invigorating, but chemical one-component therein is not fully validated all the time.Comprehensive many articles
Report and itself data, it has been found that using eurycomanone as the quassinoids material of representative and with active polysaccharide, glycopeptide and ammonia
Water-soluble bioactive component based on base acids is principle active component therein.We are according to research and put into practice confirmation, this
Two parts active ingredient is used in conjunction with having certain synergistic function.
The content of the invention
The present invention, which provides, to be had enhancing sexual function, improves fertility-rate, antifatigue, strengthen immunity of refreshing oneself, increase physical efficiency, is slow
Solve the Tongkat Ali combination of active principles preparation method of gout.This method be not used only ultrasonic extraction with aqueous solution active ingredient and
Select macroreticular resin to carry out adsorbing separation and UF membrane, remain bioactivity and the selection of Tongkat Ali effective components in plants
Property it is enriched with.
The Tongkat Ali active ingredient its preparation method that the present invention refers to is as follows：
With enhancing sexual function, improve fertility-rate, antifatigue, strengthen immunity of refreshing oneself, enhancing physical efficiency, the east for alleviating gout
Ali's combination of active principles preparation method is removed from office, mixes with aqueous solution after Tongkat Ali is crushed, is carried by continuous flow upstream ultrasound water
Processing is taken, obtains Tongkat Ali aqueous extract；
A. aqueous extract filter centrifugation is directly over macroporous resin adsorption after removing precipitation, and resin column is through eluent after absorption
Elute and collect target components, ethanol precipitation removes impurity, is concentrated and dried, and obtains Tongkat Ali group of effective components A；
B. efflux of the aqueous extract after macroporous resin adsorption, is classified using filter membrane and separated, and collects target components, decompression
Concentration, freeze-drying or spray drying, obtain Tongkat Ali group of effective components B；
C. according to a certain percentage, Tongkat Ali group of effective components A and B are mixed, Tongkat Ali combination of active principles is made.
Specially the mass volume ratio of Tongkat Ali and aqueous solution is 1: 1~30, mixture continuous ultrasound extraction 5min~
5h, ultrasonic extraction temperature are 10~70 DEG C.After Tongkat Ali ultrasound aqueous extract filter centrifugation, supernatant is rich with macroreticular resin
Collection, resinous type preferred HP-20, D101, DM130, AB-8.Macroporous resin column is using in ethanol, ethyl acetate, water after absorption
One or more of solvent mixing gradient elutions.The eluent containing quassinoids target component (eurycomanone) is collected, is concentrated under reduced pressure,
Absolute ethyl alcohol precipitates 2 removal impurity, finally obtained Tongkat Ali group of effective components A.Efflux warp after macroporous resin adsorption,
Using membrane filtering method, the component such as separation Tongkat Ali water-soluble active polysaccharide, glycopeptide and amino acid, spraying or freeze-drying, obtain
Obtain Tongkat Ali group of effective components B.Tongkat Ali group of effective components A is proportionally mixed with Tongkat Ali group of effective components B, shape
Into Tongkat Ali combination of active principles.Multiple components coexist in Tongkat Ali combination of active principles made from this method, characteristic body
Matter quassinoids component content is 1~50%, and the nutrient composition content such as various active polysaccharide, glycopeptide, amino acid is 1~20%.
Tongkat Ali combination of active principles prepared by the present invention has enhancing sexual function, improves fertility-rate, refreshes oneself that antifatigue, enhancing is immune
Power, enhancing physical efficiency, alleviate pain wind action.
Extracting temperature is controlled between 10~70 DEG C using the new technology of the present invention first, assisting ultrasonic extraction side
Method, can retain the activity of two class material compositions.The Tongkat Ali combination of active principles technological process of preparation is easy, production process
Safe and environment-friendly, effective component extraction rate, yield are high, are adapted to large-scale production.Secondly propose there are Tongkat Ali different parts
The method that effect component is used in mixed way prepares Tongkat Ali combination of active principles, strengthens overall drug effectiveness.The east of production
Ali's combination of active principles is removed from office by many-sided experimental verification, there is enhancing sexual function, improve fertility-rate, antifatigue, increasing of refreshing oneself
Strong immunity, enhancing physical efficiency, alleviate pain wind action, can be applied to male's non-organic sexual function declines, stress is big,
The crowds such as easy fatigue and the elderly of climacteric syndrome.
Embodiment
The present invention is further illustrated in following specific embodiment, this does not limit the scope of the invention.Not
In the case of departing from the above-mentioned technological thought of the present invention, according to ordinary skill knowledge and customary means, make various replacements and
Change, should all be included within the scope of the invention.
1. preparation method
The 100.0 grams of crushing of Tongkat Ali root are taken, add distilled water 2L immersions, water temperature is controlled at 50 DEG C.Utilize ultrasonic extraction
Equipment, the Tongkat Ali of crushing is mixed with water and carry out continuous ultrasound extraction 2 it is small when, then by filtering, centrifugation, obtain east leather
Ali's aqueous extract.The aqueous extract of Tongkat Ali directly uses D101 resins circulation absorption to saturation, with ethanol water pair
Adsorption column is eluted, and collects quassinoids target component, is obtained ethanol water eluent, is concentrated under reduced pressure, and it is thick to obtain Tongkat Ali
4.4 grams of product.Tongkat Ali crude product is dissolved with absolute ethyl alcohol, is filtered the precipitation for removing and producing, is obtained Tongkat Ali active ingredient second
Alcoholic solution, is concentrated under reduced pressure, and further obtains Tongkat Ali group of effective components A.Trickle is set using film after macroporous resin adsorption
Standby filtering, separates by microfiltration membranes, ultrafiltration membrane, NF membrane etc., collects NF membrane and ultrafiltration membrane retention component, separates work therein
Property polysaccharide, glycopeptide and amino acid, it is final to obtain 1.2g Tongkat Ali group of effective components B.Tongkat Ali is mixed according to 2: 1 ratio
Group of effective components A and B, are prepared into Tongkat Ali combination of active principles.
2. Mouse Acute Toxicity is tested
Tongkat Ali combination of active principles is given to ICR mouse according to intraperitoneal injection mode, experiment obtains approximate cause
Dead dosage, maximal non-toxic dosage.Male mice approximate lethal dose scope is 100.0-120.0mg/kg, maximal non-toxic agent
Measure as 80mg/kg；Female mice approximate lethal dose scope is 180.0-225.0mg/kg, and maximal non-toxic dosage is 80mg/
kg.Dead animal is dissected, visually observes Other Main Internal Organs no abnormality seen pathological change.
Tongkat Ali combination of active principles is given to ICR mouse according to gastric infusion mode, the results showed that administration 500mg/
Kg, mouse do not find that toxicity shows.
Claims (7)
1. preparing the method for Tongkat Ali (also known as eastern brother Ali) combination of active principles, its process is：After Tongkat Ali is crushed
Mixed with aqueous solution, at a certain temperature, by continuous flow upstream ultrasound water extraction process, obtain Tongkat Ali aqueous extract, institute
The mass volume ratio of Tongkat Ali and aqueous solution is 1 during stating continuous flow upstream ultrasonic extraction:1~30, mixture continuous ultrasound
5min~5h is extracted, ultrasonic extraction temperature is 10~70 DEG C；
A. aqueous extract filter centrifugation is directly over macroporous resin adsorption after removing precipitation, and resin column elution is simultaneously after absorption
Target components are collected, ethanol precipitation removes impurity, and clear liquid is concentrated and dried, and obtains Tongkat Ali group of effective components A, group of effective components
A is to contain quassinoids material；
B. efflux of the aqueous extract after macroporous resin adsorption, is classified using filter membrane and separated, and collects target components, and decompression is dense
Contracting, drying, it is water-soluble active polysaccharide, glycopeptide and amino acid to obtain Tongkat Ali group of effective components B, group of effective components B；
C. according to a certain percentage, mix Tongkat Ali group of effective components A and B, Tongkat Ali combination of active principles is made, effectively into
Multiple components coexist in subassembly, and quassinoids component content is 1~50%, water-soluble active polysaccharide, glycopeptide, amino acid content
1~20%.
2. the preparation method of Tongkat Ali combination of active principles according to claim 1, its raw material is Tongkat Ali tree root.
3. the preparation method of Tongkat Ali combination of active principles according to claim 1, its enrichment characteristics is aqueous extract
It is enriched with macroporous resin adsorption, resinous type HP-20, D101, DM130, AB-8.
4. the preparation method of Tongkat Ali combination of active principles according to claim 1, the elution of its resin column adsorption component is special
Levy one or more of solvent mixing gradient elutions in ethanol, ethyl acetate, water is used in resin column.
5. the preparation method of Tongkat Ali combination of active principles according to claim 1, its filter membrane classification separation selection micro-filtration
Film, ultrafiltration membrane, reverse osmosis membrane and the processing of sodium filter membrane one or more gradient.
6. the preparation method of Tongkat Ali combination of active principles according to claim 1, the quassinoids material is wide tassel
Ketone, content is 1~35%.
7. Tongkat Ali combination of active principles described in claim 1 prepare enhancing sexual function, improve fertility-rate, refresh oneself it is anti-tired
Application in labor, strengthen immunity, increase physical efficiency, alleviation gout medicine.
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| CN109456418B (en) * | 2019-01-17 | 2020-10-27 | 齐齐哈尔大学 | A kind of active Tongkat Ali polysaccharide and preparation method thereof |
| CN111303180A (en) * | 2020-02-21 | 2020-06-19 | 湖南中茂生物科技有限公司 | Method for extracting eurycomanone from eurycoma longifolia |
| CN112843050B (en) * | 2021-02-02 | 2022-11-25 | 天津中医药大学 | Application of quassin diterpenoid in preparation of medicine for treating cholestatic liver disease |
| CN113425765A (en) * | 2021-07-14 | 2021-09-24 | 重庆森康道生命健康研究院有限公司 | Uric acid reducing medicine and preparation method thereof |
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