CN107260807A - A kind of complex injection of Chinese materia medica and preparation method for treating Haemophilus parasuis - Google Patents
A kind of complex injection of Chinese materia medica and preparation method for treating Haemophilus parasuis Download PDFInfo
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Abstract
The invention discloses a kind of complex injection of Chinese materia medica for treating Haemophilus parasuis, extracted and be prepared from through water extraction and alcohol precipitation method by honeysuckle, dandelion, radix scutellariae, the bighead atractylodes rhizome, radix glycyrrhizae.Compared with prior art, there is good synergistic function between the Chinese medicine component of the present invention, it is extremely sensitive to haemophilus parasuis, and immunity of organisms can be strengthened, comprehensive coordinate physiological metabolism, play a part of jointly it is clearing heat and detoxicating, soothing the liver expel the heat-evil, moisten the lung and relieve the cough, eliminating dampness and eliminating phlegm, invigorating the spleen tonifying Qi, antibacterial bacteriostatic.The present invention has the advantages that simple, easy to use preparation technology, drug residue free and toxic side effect, effect are good, and more than 85% is can reach to the cure rate of haemophilus parasuis.
Description
Technical field
The invention belongs to field of veterinary, it is related to a kind of complex injection of Chinese materia medica for treating Haemophilus parasuis and its preparation
Method.
Background technology
Haemophilus parasuis, also known as pig polyserositis draw Se Shi diseases with arthritis or leather（Glasser,s
disease）, it is the conditionity infectiousness of a boar caused by haemophilus parasuis (Haemophilus parasuis, HPS)
Disease.This bacterium is normal in bacterium in the pig upper respiratory tract, and Fit First pig can turn into the infection sources.Source of parents is lost after group, wean is turned
Antibody is protected, when Other diseases cause immunity degradation, may occur in which symptom, is discharged pathogen, is mainly passed through respiratory infectious.Should
Disease only occurs in pig, can be infected from the growing and fattening pigs of 2 week old piglets to 4 monthly ages, but be more common in the child care pig of 5-8 week old.Face
Raised on bed with body temperature, generate heat, cough, have difficulty in breathing, become thin, arthrocele and dyskinesia are characterized, a small number of pig performance god
Through symptom, the death rate may be up to 50% when being in a bad way.In addition, the disease can also cause septicemia, and may after acute infection
Leave sequelae, i.e. Sow abortion, the chronic limping of boar.The sick Chang Bingfa or secondary to Eaton agent pneumonia, swine flu, pseudo- mad dog
Disease, porcine reproductive and respiratory syndrome or Porcine circovirus desease etc., complicate the state of an illness of swinery, death rate increase, economic loss
Aggravate.This disease is《Pig slaughtering quarantine code》One of 13 kinds of diseases of legal quarantine object, therefore Haemophilus parasuis is
It is increasingly becoming the typical bacteria disease of influence pig industry in world wide.
At present, it is main both at home and abroad that this disease is prevented and treated by antibiotic medicine preventing and treating and vaccine immunity.Clinically commonly use antibiosis
Plain spice or the mode of injection are prevented or treated to whole swinery, sometimes due to effect is bad, can also escalated dose it is blind
Mesh abuse of antibiotics, not only causes the pathogen to produce drug resistance to many antibiotic, also results in antibiotic residue in pig body, shadow
Ring food security.Although the country has been developed that polyvalent inactivation seedling, because haemophilus parasuis has obvious region feature, no
Cross protection rate between homologous serotype is very low, therefore each pig farm reflection of protecting effect of commercially available vaccine differs.To sum up, based on this
Harmfulness and the death rate of the disease in pig industry are higher, and can not play good prevention effect using antibiotic and vaccine,
It is badly in need of a kind of medicine safe, without resistance, noresidue that can be good at preventing and treating the disease of research and development.
With the reinforcement of people's health consciousness, toxic side effect is small, the Chinese medicine preparation of green non-pollution noresidue more and more by
To the attention of people.Chinese herbal medicine belongs to natural drug, with medicinal, nutrition, originate wide, low cost, low toxicity and low residue, is difficult production
The features such as raw drug resistance, and Chinese medicine contains plurality of active ingredients, such as alkaloid, organic acid, saponin(e, polysaccharide, sesquiterpene lactone, Huang
Ketone, phytosterol etc., with antibacterial, the antiviral, effect of diminishing inflammation etc., and can adjust the immunity function of body, improve
Resistivity of the pig to cause of disease.Research carries out the development of Chinese herbal antibacterial preparation for treatment haemophilus parasuis and other bacteriums
The preventing and treating of property disease is significant.
Therefore, the invention provides a kind of reasonable recipe, simple preparation, strong drug action, safe and noresidue treatment are secondary
The complex injection of Chinese materia medica of haemophilus suis disease.
The content of the invention
It is an object of the invention to provide complex injection of Chinese materia medica for the treatment of Haemophilus parasuis and preparation method thereof, it is
Above-mentioned purpose is realized, the present invention uses following technical scheme：
A kind of complex injection of Chinese materia medica for treating Haemophilus parasuis, by the Chinese medicine with following weight parts through water extraction and alcohol precipitation method
The traditional Chinese medicine injection for extracting and being made：6-10 parts of honeysuckle, 6-10 parts of dandelion, 5-8 parts of radix scutellariae, 3-6 parts of the bighead atractylodes rhizome, radix glycyrrhizae 2-5
Part, the every 1ml decoctions of prepared parenteral solution are equivalent to primary crude drug 3.0g.
It is further preferred that each component part by weight is：8 parts of honeysuckle, 8 parts of dandelion, 7 parts of radix scutellariae, 4 parts of the bighead atractylodes rhizome is sweet
3 parts of grass.
The invention also discloses a kind of preparation method for the complex injection of Chinese materia medica for treating haemophilus parasuis, according to following
Step is carried out：
（1）Honeysuckle is weighed, 8-10 times of 15% alcohol reflux measured is added and extracts 2 times, each 1 hour every time, merge extract solution, subtract
Pressure is concentrated into the clear cream that relative density is 1.15-1.20, plus ethanol makes alcohol content up to 65%, stands 24h, and filtration, filter residue adds ethanol
Make alcohol content up to 65%, stand 24 hours, filtration merges filtrate twice, is concentrated under reduced pressure, the gained volume of concentrate is total
The 1/10 of filtrate, is refrigerated standby；
（2）Dandelion, radix scutellariae, the bighead atractylodes rhizome, radix glycyrrhizae are weighed, is merged with the dregs of a decoction of honeysuckle, 4-10 times of water of gross weight is added, 2 are decocted
Secondary, each 1.5h, collecting decoction, filtration, filtrate is concentrated into the clear cream that relative density is 1.12-1.15, plus ethanol makes alcohol content
Stand 48 hours up to 75%, 4 DEG C, filtration, filtrate decompression is concentrated into no alcohol taste, add appropriate water for injection, regulation pH value to
About 8.0；
（3）By step 1) in concentrate add step 2）Decoction in, and stirring and dissolving adds 0.5% activity
Charcoal, is handled 30 minutes in 60 DEG C, and filtration obtains filtrate standby；
（4）Filtrate adds appropriate phenmethylol, sodium hydrogensulfite, Tween-80, and PH is adjusted to 6.5 with 8% sodium hydroxide solution, and
Plus injection appropriate amount of water so that prepared parenteral solution, equivalent to primary crude drug 3.0g, stirs per 1ml decoctions, filters, and fills
Envelope, sterilizing, are produced.
The application method of complex injection of Chinese materia medica of the present invention：According to pig per 1kg body weight 0.1ml, intramuscular injection, 1 time a day,
It is used in conjunction 3-5 days.
Each component pharmacological effect is as follows in prescription of the present invention：
Honeysuckle, it is sweet, it is cold in nature.There is clearing heat and detoxicating, dispelling wind and heat from the body；Cure mainly seasonal febrile diseases heating, anemopyretic cold, throat swell
Bitterly, cough with lung heat, toxic-heat and blood stasis, carbuncle sore tumefacting virus etc..Modern age pharmaceutical research shows, honeysuckle is a kind of very strong broad-spectrum antiseptic
Chinese medicine, the also effect with booster immunization function.
Dandelion, it is bitter, sweet, it is cold in nature.Have effects that clearing heat and detoxicating, clearing heat and promoting diuresis, it is soothing the liver expel the heat-evil, tonneau urine,
With very strong bactericidal action；Cure mainly heat toxin carbuncle swells, jaundice with damp-heat pathogen, puckery pain of heat gonorrhea, urinary tract occlusion etc..
Radix scutellariae, bitter is cold in nature.There is heat-clearing and damp-drying drug, purging intense heat and detonicating, hemostasis, also there is very strong antibacterial, anti-inflammatory suppression
The effect of bacterium；Cure mainly cough with lung heat, damp and hot stomach and intestine, rush down dysentery, jaundice, have blood in stool, red eye, swell pain, carbuncle sore tumefacting virus etc..
The bighead atractylodes rhizome, sweet, hardship is warm-natured.With diuresis, the generation of hydrops can be reduced；Air making-up and spleen enlivening, improves body and resists
Sick ability；Also have eliminating dampness and eliminating phlegm, expelling wind and clearing away cold, the antiabortive effect of strengthening exterior and reducing sweat, QI invigorating, and antibacterial, antineoplastic effect in addition.
Cure mainly deficiency-weakness of spleen-QI, insufficiency of the spleen stagnant, phlegm retention anti-dazzle nervous, spontaneous perspiration, oedema, wind-cold-dampness arthralgia etc..
Radix glycyrrhizae, sweet, mild-natured, clearing heat and detoxicating with invigorating the spleen and benefiting qi, expelling phlegm and arresting coughing, nvigorating the spleen and in is mainly used in taste empty
Weak abdominal distension and anorexia, it is weak；Coordinating the drug actions of a prescription, the toxicity for alleviating some drugses is strong, mitigates toxic side effect of the medicine to body.
Honeysuckle, dandelion in formula be respectively provided with it is clearing heat and detoxicating, soothing the liver expel the heat-evil, clearing lung-heat heat cough effect, and be respectively provided with
Stronger bactericidal action, and honeysuckle can also booster immunization function, two medicines combination be monarch drug in a prescription, clearing heat and detoxicating, bactericidal action is more
By force.Radix scutellariae, the bighead atractylodes rhizome, radix glycyrrhizae are ministerial drug.Radix scutellariae heat-clearing and damp-drying drug, purging intense heat and detonicating, cough with lung heat is controlled, the bighead atractylodes rhizome is eliminating dampness and eliminating phlegm, wind-dispelling is scattered
Cold, air making-up and spleen enlivening, the two has antibacterial action concurrently, a bitter temperature, a bitter cold, share can heat-clearing and damp-drying drug, invigorating the spleen is antiabortive.Bighead atractylodes rhizome hardship temperate-dryness
It is wet, can tonifying spleen sun, strong fortune temper, radix glycyrrhizae tonifying Qi and in, moisten the lung and relieve the cough, two medicines share tool nvigorating the spleen and in, adjust and mend the foundation of acquired constitution work(
Effect.Radix glycyrrhizae can also coordinating the drug actions of a prescription, the toxicity for alleviating medicine is strong, mitigates medicine to the toxic side effect of body.All medicine collaborations above
Synergy, has preferably prevention and treatment effect to Haemophilus parasuis.
Specific embodiment
The invention will be further described with reference to embodiments, but the present invention is not limited only to these embodiments, is not taking off
On the premise of from present inventive concept, any change made is all fallen within the scope of the Protection Code of the present invention.
The complex injection of Chinese materia medica formula of embodiment 1
Formula 1：Honeysuckle 8kg, dandelion 8kg, radix scutellariae 7kg, bighead atractylodes rhizome 4kg, radix glycyrrhizae 3kg, altogether 30kg；
Formula 2：Honeysuckle 8kg, dandelion 8kg, radix scutellariae 6kg, bighead atractylodes rhizome 4kg, radix glycyrrhizae 4kg, altogether 30kg；
Formula 3：Honeysuckle 8kg, dandelion 7kg, radix scutellariae 7kg, bighead atractylodes rhizome 4kg, radix glycyrrhizae 4kg, altogether 30kg；
Formula 4：Honeysuckle 9kg, dandelion 8kg, radix scutellariae 7kg, bighead atractylodes rhizome 3kg, radix glycyrrhizae 3kg, altogether 30kg.
The preparation method of embodiment 2
（1）Honeysuckle is weighed, 8-10 times of 15% alcohol reflux measured is added and extracts 2 times, each 1 hour every time, merge extract solution, subtract
Pressure is concentrated into the clear cream that relative density is 1.15-1.20, plus ethanol makes alcohol content up to 65%, stands 24h, and filtration, filter residue adds ethanol
Make alcohol content up to 65%, stand 24 hours, filtration merges filtrate twice, is concentrated under reduced pressure, the gained volume of concentrate is total
The 1/10 of filtrate, is refrigerated standby；
（2）Dandelion, radix scutellariae, the bighead atractylodes rhizome, radix glycyrrhizae are weighed, is merged with the dregs of a decoction of honeysuckle, 4-10 times of water of gross weight is added, 2 are decocted
Secondary, each 1.5h, collecting decoction, filtration, filtrate is concentrated into the clear cream that relative density is 1.12-1.15, plus ethanol makes alcohol content
48 hours are stood up to 75%, 4 DEG C, filtration, filtrate decompression is concentrated into no alcohol taste, add appropriate water for injection to 5L, adjust PH
It is worth to about 8.0；
（3）By step 1) in concentrate add step 2）Decoction in, and stirring and dissolving adds 0.5% activity
Charcoal, is handled 30 minutes in 60 DEG C, and filtration obtains filtrate standby；
（4）Filtrate adds 100ml phenmethylols, 20g sodium hydrogensulfites, 50ml Tween-80s, and PH is adjusted with 8% sodium hydroxide solution
To 6.5, and inject water to 10L so that prepared parenteral solution is per 1ml decoctions equivalent to primary crude drug 3.0g, and stirring is equal
It is even, filtration, embedding, sterilizing are produced.
The application effect of embodiment 3 is tested
In order to verify the complex injection of Chinese materia medica Clinical practice security and the therapeutic effect to Haemophilus parasuis of the present invention,
Further illustrated by tests below：
1 acute toxicity test
1.1 trial test
20 mouse are taken, trial test is carried out according to sequential method, Continuous Observation 7d measures mouse peritoneal injection
100% lethal dose is 34ml/kg, i.e. 102000mg/kg.
1.2 LD50Measure
20 ± 2g of body weight healthy mices 50 are taken, random packet, 10/group, male and female half and half, artificial light 12h, breeding observing
7d, in water is can't help in fasting in 4h after 12h before administration and administration；Injected during experiment with suitable dosage disposable celiac, injection volume is
0.2ml/10g body weight, group number is 5, i.e., away from for 0.7, observing 7d, the poisoning symptom of record each group mouse, death time and death
Quantity, calculates LD50。
1.3 result：There is acute poisoning symptom in individual mice after intraperitoneal injection, have that volt is lower few dynamic, activity is difficult, do not eat,
The phenomenon such as it is short of breath, it is dead in 0.5-5h after the administration of high dose group；Also there are sporadic deaths in other groups, and the visible heart of cut open inspection swells
Big congested, liver is congested, mesenterium is congested, intestinal edema.8.1634mg/kg dosage groups mouse is without almost adverse reaction.Experiment knot
Really improved karber's method is calculated, LD50=log-1【Xm-d（∑p-0.5）】, LD50=16.076ml/kg。
The median lethal dose result of table 1
2 target animals safety testings
2.1 experimental animal
Sodium selenite 32,20 ± 2kg of average weight.
2.2 test method
Piglet is randomly divided into 4 groups, every group 8, pre- under same rearing conditions to raise 7 days, piglet still health can be tested.
By the 1st, 2,3 groups be set to test group, intramuscular injection traditional Chinese medicine 0.1ml/kg, 0.3ml/kg, 0.5ml/kg of the present invention respectively,
1 time a day, it is used in conjunction 5 days；4th group is blank control group, according to 0.5ml/kg injecting normal salines, 1 time a day, is used in conjunction 5 days.
Continuous Observation 15 days after piglet medications, record adverse reaction, the feelings such as drinking-water, the state of mind and changes of weight of searching for food are tested to all
Condition.
2.3 result of the test
Compared with control group, obvious adverse reaction, feeding, drinking-water, the state of mind, work do not occur for experiment 1,2,3 groups of piglets
Dynamic normal, average weight development difference is not notable.
3 muscle irritations are tested
3.1 test method
Take body weight 2kg or so healthy rabbits 4, male and female half and half, using consubstantiality left and right sides self-contrast method.Cut off rabbit both sides
The hind leg quadriceps muscle of thigh position rabbit hair, sterilizes latter side injection parenteral solution 0.2ml/ of the present invention (kg is only), the body such as another side injection
Long-pending physiological saline is compared.Experimental rabbits should be as healthy as usual after 48h, put to death 2 by auricular vein injection air, dissection is taken out
Injection site muscle, it is longitudinally slit, reaction and the decoction absorbing state of injection site musculature are observed, the order of reaction is determined,
Check whether injection site produces the irritative responses such as hyperemia, oedema, lump, denaturation or necrosis.
Table 2 stimulates series to classify
As a result：The both sides of injecting normal saline and parenteral solution of the present invention have slight swelling, rabbit to have no stock after putting to death dissection
Musculus quadriceps has congested, lump and degeneration necrosis, and stimulate the reaction series is 1 grade, and parenteral solution of the present invention can be used as intramuscular injection
Agent is used.
4 heat source checks
4.1 test method
The version veterinary drug allusion quotation selection in 2015 of the reference People's Republic of China (PRC) is applicable rabbit, and carries out preparation before experiment.
Applicable rabbit 3 is taken, slowly injects and is warmed to from ear vein in the 15min after determining its normal body temperature
About 38 DEG C of traditional Chinese medicine injection of the present invention（0.1mL/kg body weight）, then determine body temperature 1 time every 30min, 6 surveyed altogether
It is secondary, normal body temperature is once subtracted with highest in 6 body temperature, is the rise temperature of the rabbit body temperature.In 3 rabbit
There is 1 body temperature to raise 0.6 DEG C or more than 0.6 DEG C, or 3 rabbit body temperature rises are below 0.6 DEG C, but body temperature is elevated
Summation separately takes 5 rabbit retrials, inspection method is ibid up to 1.4 DEG C or more than 1.4 DEG C.
Criterion：In 3 rabbit of preliminary examination, body temperature rise is below 0.6 DEG C, and 3 rabbit body temperature rises
Summation is less than 1.4 DEG C；Or in 5 rabbit of retrial, the rabbit that body temperature raises 0.6 DEG C or more than 0.6 DEG C is no more than 1
, and preliminary examination, the body temperature rise summation of retrial 8 rabbit of merging are 3.5 DEG C or less than 3.5 DEG C, think test sample
Pyrogen test meet regulation.
In 3 rabbit of preliminary examination, body temperature raises 0.6 DEG C or more than 0.6 DEG C of rabbit more than 1；Or in retrial
5 rabbit in, body temperature raises 0.6 DEG C or more than 0.6 DEG C of rabbit more than 1；Or merge 8 in preliminary examination, retrial
The body temperature of rabbit raises summation more than 3.5 DEG C, thinks that the pyrogen test of test sample does not meet rule.Rabbit heating is negative value
When, in terms of 0 DEG C.
4.2 result of the test
3 rabbit of preliminary examination are tested, body temperature rise is below 0.6 DEG C, and 3 rabbit body temperature rise summations are less than 1.4 DEG C, it is believed that
The pyrogen test of traditional Chinese medicine injection of the present invention meets regulation.
5 Chinese herbal medicine drug sensitive tests
The separation identification of 5.1 pathogens
Sick pig cavum pericardiale exudate, liver etc. in two doubtful haemophilus parasuis morbidity pig farms, aseptic collection death 6-8h
It is inoculated in 37 DEG C of culture 48h of chocolate agar medium.Neat in edge is grown on chocolate agar medium, it is small and saturating
Bright bacterium colony, has no haemolysis.By this bacterium colony smear, Gram's staining, microscopy has Gram-negative to differ in size, short and small bar
Shape, club shape, very thin shaft-like bacterium.The above-mentioned typical bacterium colony of picking was continuously cultivated for 2~3 generations again, not miscellaneous during microscopy
Bacterium, 4 DEG C of preservations are stand-by.
Satellite Experiment：Colonies typical on picking chocolate agar plate, is inoculated on 2 rabbit blood agar plates,
The row staphylococcus aureus of streak inoculation two on one of rabbit blood flat board, another cultivates 48h, golden yellow as control
Color staphylococcus line surrounding growth is in obvious " satellitism ", and nearer offline, bacterium colony is bigger, and slightly remote bacterium colony is smaller.Simultaneously
Take the haemophilus parasuis type strain that laboratory preserves with method culture, same " satellitism " occur, illustrate the bacterial strain of separation
It is consistent with the cultural character of haemophilus parasuis type strain.It can be determined that two pig farm isolated strains are haemophilus parasuis.
5.2 Odontothrips loti drug sensitive tests
It is prepared by decoction：Traditional Chinese medicine injection of the present invention, crude drug content 3g/ml；Each simple is according to corresponding in preferably prescription of the invention
Dosage, is manufactured separately decoction, standby.
In " super-clean bench ", the haemophilus parasuis nutrient solution separated in right amount with the oese picking through flame sterilization connects
Plant in a small amount of physiological saline, it is 10 that concentration, which is made,5Cfu/ml bacteria suspension, with sterile cotton swabs by bacteria suspension even compact
Media surface is coated with, and the Oxford cup of sterilizing is placed on by media surface with sterile working, light pressure makes it be connect with culture medium
Tight is touched, each plate can be put after 4-6 Oxford cup, several minutes, add different decoctions to be checked into each Oxford cup respectively,
Make decoction full without flowing over, and carry out mark.Put 37 DEG C to cultivate 8-18 hours, observe result.
As a result criterion：Antibacterial circle diameter（mm）, more than 20, it is extremely quick；15-20, Gao Min；It is quick in 10-14；Less than 10,
Muting sensitive；0, it is unwise.
5.3 result of the test
The traditional Chinese medicine drug sensitive test result of the present invention of table 3
Drug sensitivity tests illustrate that traditional Chinese medicine injection of the present invention belongs to high sensitizing drug to haemophilus parasuis, can apply to clinical pair
The treatment of haemophilus suis disease.
Therapeutic effect of 6 medicines of the present invention to clinical Haemophilus parasuis example
2016.12 middle of the month, the bull child care pig of Liaoning Shenyang Liaozhong pig farm about 200（5-8 week old）, there is breathing tired in morbidity
It is difficult, cough, become thin, the thick random symptom of hair, indivedual sick pig arthroceles, walk lamely, Separate acts are difficult, symptom occur to dead
Comparatively fast.Cut open inspection finds that articular cavity has mucus, chest and abdomen hydrops, heart surface cellulosic attachment, in cor villosum, lungs and thoracic cavity
Diaphram adhesion, lobe of the lung tubercle and in carnification, doubtful Haemophilus parasuis.The pig farm sick pig totally 80 is selected, is divided into four groups
Carry out therapeutic test.Laboratory carries out bacteria distribution identification simultaneously, and is diagnosed as haemophilus parasuis infection.
2017.1 months, the pig farm of Fuxin Area, China one, 100 bulls（6-8 week old）Piglet falls ill, and sick pig becomes thin, appetite subtracts
Move back, pant, coughing, having difficulty in breathing, there is mucus sexual secretion in nostril, symptom 3d or so death occurs in serious patient.Cut open inspection is sent out
Existing lungs surface have a large amount of fibrinous exudates and with thoracic cavity adhesion, interstitial pneumonia and carnification, inguinal lymph nodes swells
Massive haemorrhage, doubtful Haemophilus parasuis.The pig farm sick pig 80 is selected, is divided into four groups.Laboratory carries out bacteria distribution simultaneously
Identification, and it is diagnosed as haemophilus parasuis infection.
Experiment packet and administration：
The experiment of table 4 packet and administration
Test results and analysis：
The traditional Chinese medicine therapeutic effect -1 of the present invention of table 5
The traditional Chinese medicine therapeutic effect -2 of the present invention of table 6
Note：Infect control group and cure head number, refer to pig without administration, indivedual symptoms are lighter, self-healing.
Result of the test finds that traditional Chinese medicine injection of the present invention is controlled on the haemophilus parasuis morbidity pig farm of Liaoning Area 2
Treat, cure rate can reach more than 85%, and medicine of the present invention is contrasted with ceftiofur sodium, Enrofloxacin, and therapeutic effect is more excellent.Explanation
Traditional Chinese medicine of the present invention absorbs fast, curative effect rapidly, there is preferable therapeutic effect to haemophilus parasuis.
To sum up, the lethal dose of mouse peritoneal injection 100% of traditional Chinese medicine of the present invention is 34ml/kg, according to present invention note
Penetrate the intraperitoneal injection of agent concentration 3g/ml, 0.4ml/20g body weight, median lethal dose LD50=16.076ml/kg；Using concentration 3g/ml medicines
Liquid carries out drug sensitive experiment to the haemophilus parasuis being clinically separated, and inhibition zone size is 17mm, extremely sensitive；Using 5 times of clinics
Consumption 0.5ml/kg body weight, carries out the safety testing of target animals pig, 1 time a day, is used in conjunction 5 days, the performance of test pig physiological characteristic
Normally.The swinery for treating clinical early infection haemophilus parasuis using dosage 0.1ml/kg body weight is treated, with preferable
Effect, cure rate can reach more than 85%.Chinese medicine compound prescription parenteral solution of the present invention, its composition is pure natural medical, containing a variety of
Active ingredient, there is good synergistic function for pathogen, does not produce drug resistance, safe, noresidue and the secondary work of poison
With, Clinical practice definite effect, and the convenient production of preparation technology, it can be easy to promote the use of.
Claims (6)
1. a kind of complex injection of Chinese materia medica for treating Haemophilus parasuis, it is characterised in that be made up of following raw material：Gold and silver
Flower, dandelion, radix scutellariae, the bighead atractylodes rhizome, radix glycyrrhizae.
2. complex injection of Chinese materia medica according to claim 1, it is characterised in that each component content is：Honeysuckle 6-
10 parts, 6-10 parts of dandelion, 5-8 parts of radix scutellariae, 3-6 parts of the bighead atractylodes rhizome, 2-5 parts of radix glycyrrhizae.
3. complex injection of Chinese materia medica according to claim 1 or 2, it is characterised in that each component content is：Honeysuckle
8 parts, 8 parts of dandelion, 7 parts of radix scutellariae, 4 parts of the bighead atractylodes rhizome, 3 parts of radix glycyrrhizae.
4. the complex injection of Chinese materia medica according to any one of claim 1 ~ 3, it is characterised in that each component content is：
Honeysuckle 8kg, the kg of dandelion 8, the kg of radix scutellariae 7, the kg of the bighead atractylodes rhizome 4, the kg of radix glycyrrhizae 3.
5. a kind of preparation method for the complex injection of Chinese materia medica for treating Haemophilus parasuis, it is characterised in that including following step
Suddenly：
1）6-10 parts of honeysuckle is weighed, 8-10 times of 15% alcohol reflux measured is added and extracts 2 times, each 1 hour every time, merge and extract
Liquid, is concentrated under reduced pressure into the clear cream that relative density is 1.15-1.20, plus ethanol makes alcohol content up to 65%, stands 24h, filtration, filter residue
Plus ethanol makes alcohol content up to 65%, stands 24 hours, filtration merges filtrate twice, is concentrated under reduced pressure, gained concentrated liquid
Product is the 1/10 of total filtrate, is refrigerated standby；
2）6-10 parts of dandelion is weighed, 5-8 parts of radix scutellariae, 3-6 parts of the bighead atractylodes rhizome, 2-5 parts of radix glycyrrhizae merges with the dregs of a decoction of honeysuckle, added
4-10 times of water of gross weight, is decocted 2 times, each 1.5h, collecting decoction, filtration, and it is 1.12-1.15's that filtrate, which is concentrated into relative density,
Clear cream, plus ethanol make alcohol content stand 48 hours up to 75%, 4 DEG C, and filtration, filtrate decompression is concentrated into no alcohol taste, add appropriate
Water for injection, regulation pH value to about 8.0；
3）By step 1) in concentrate add step 2）Decoction in, and stirring and dissolving adds 0.5% activated carbon, at 60 DEG C
Reason 30 minutes, filtration, obtains filtrate standby；
4）Filtrate adds appropriate phenmethylol, sodium hydrogensulfite, Tween-80, adjusts PH to 6.5 with 8% sodium hydroxide solution, and add
Appropriate water for injection so that prepared parenteral solution, equivalent to primary crude drug 3.0g, stirs per 1ml decoctions, is filtered, and is filled
Envelope, sterilizing, are produced.
6. a kind of complex injection of Chinese materia medica method in clinical application for treating Haemophilus parasuis, it is characterised in that every according to pig
1kg body weight 0.1ml, intramuscular injection 1 time a day, is used in conjunction 3-5 days.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117982583A (en) * | 2024-02-22 | 2024-05-07 | 贵州大学 | A Chinese herbal medicine compound preparation for preventing and controlling pig respiratory diseases, and its preparation method and application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101757106A (en) * | 2009-11-12 | 2010-06-30 | 郑州后羿制药有限公司 | Injecta for heat clearing and detoxification and preparation method thereof |
| CN104288284A (en) * | 2014-10-28 | 2015-01-21 | 河南省睿兰特生物制药有限公司 | Traditional Chinese medicine compound hydro-acupuncture preparation for preventing and curing fever and plague of livestock and poultry and preparation method thereof |
| CN104288317A (en) * | 2014-09-30 | 2015-01-21 | 天津瑞贝特科技发展有限公司 | Traditional Chinese medicine veterinary injection with antiviral, antibacterial and anti-inflammatory effects and preparation method thereof |
| US20160015762A1 (en) * | 2014-07-17 | 2016-01-21 | Jonathan Shelley Nimitz | Antiviral supplement compositions and methods of use |
-
2017
- 2017-06-14 CN CN201710445411.5A patent/CN107260807A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101757106A (en) * | 2009-11-12 | 2010-06-30 | 郑州后羿制药有限公司 | Injecta for heat clearing and detoxification and preparation method thereof |
| US20160015762A1 (en) * | 2014-07-17 | 2016-01-21 | Jonathan Shelley Nimitz | Antiviral supplement compositions and methods of use |
| CN104288317A (en) * | 2014-09-30 | 2015-01-21 | 天津瑞贝特科技发展有限公司 | Traditional Chinese medicine veterinary injection with antiviral, antibacterial and anti-inflammatory effects and preparation method thereof |
| CN104288284A (en) * | 2014-10-28 | 2015-01-21 | 河南省睿兰特生物制药有限公司 | Traditional Chinese medicine compound hydro-acupuncture preparation for preventing and curing fever and plague of livestock and poultry and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 程喜荣: "湖北省黄冈市规模猪场副猪嗜血杆菌并的流行现状及防治策略", 《中国猪业》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117982583A (en) * | 2024-02-22 | 2024-05-07 | 贵州大学 | A Chinese herbal medicine compound preparation for preventing and controlling pig respiratory diseases, and its preparation method and application |
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