CN107176879A - One kind makees matrix with mushroom mushroom bran using oblique top bacterium and prepares bio-bacterial manure and method - Google Patents
One kind makees matrix with mushroom mushroom bran using oblique top bacterium and prepares bio-bacterial manure and method Download PDFInfo
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- CN107176879A CN107176879A CN201710478582.8A CN201710478582A CN107176879A CN 107176879 A CN107176879 A CN 107176879A CN 201710478582 A CN201710478582 A CN 201710478582A CN 107176879 A CN107176879 A CN 107176879A
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- 238000000034 method Methods 0.000 title claims abstract description 20
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- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 9
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- DLYUQMMRRRQYAE-UHFFFAOYSA-N phosphorus pentoxide Inorganic materials O1P(O2)(=O)OP3(=O)OP1(=O)OP2(=O)O3 DLYUQMMRRRQYAE-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Botany (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Pest Control & Pesticides (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Fertilizers (AREA)
Abstract
The present invention provides a kind of utilization oblique top bacterium and makees the method that matrix prepares bio-bacterial manure with mushroom mushroom bran, belong to technical field of biological fertilizer, including the shake-flask seed culture of oblique top bacterium, seed tank culture, the processing of mushroom mushroom bran, mushroom bran fermentation, connect the steps such as bacterium after fermentation culture, pelletizing forming.The invention provides oblique top bacterium zymotechnique, using the production hormone of the strong nitrogen fixation of its mycelia and generation, and be aided with by matrix of mushroom mushroom bran it is inoculum fermented bio-bacterial manure is made, with obvious economic benefits, also with obvious ecological and environmental protection effect.After this bio-bacterial manure is manured into soil; the physicochemical property of soil can also further be improved, increase soil organic matter content, promote the formation and conversion of agron and granule group; soil water-retaining performance and soil fertility are improved, promotes crops erosion resistance and volume increase.Being excessively used for chemical fertilizer, which can be reduced, causes many negative effects simultaneously, such as soil property pollution, environmental pollution.This bio-bacterial manure, which is directly used in crops, vegetables production, can not only improve yield, and the agricultural product of production are non-harmful product.
Description
Technical field
The present invention provides one kind and makees matrix preparation bio-bacterial manure using oblique top bacterium with mushroom mushroom bran, while also disclosing its system
Preparation Method, belongs to technical field of biological fertilizer.
Background technology
Oblique top bacterium (CIitopilus caespitosus Pk).Be under the jurisdiction of Mycophyta door, Basidiomycetes, Agaricales,
Bai Mo sections, oblique top Pseudomonas.One kind that oblique top bacterium (CIitopilus caespitosus Pk) is distributed across Northeast Forest Areas is delicious
Edible mushroom, summer and autumn are born in woods on the ground.In great numbers in the local plant growing way of its growth, color is emerald green brilliant, forms one substantially
Straw cord, fairy ring is done at side.It is considered that the reason for forming fairy ring is due to the increased result of nitrogenous fertilizer in soil.Prove oblique top
Bacterium mycelia has strong nitrogen fixation, and some endobacillary materials of oblique top and its metabolite-fumarase are thought in research, to planting
Thing growth has obvious stimulation effect.
Mushroom mushroom bran is remaining discarded object after cultivation, mushroom mycelium residuum and is digested through mushroom mycelium, and matter occurs for structure
The compound of the compositions such as the crude fibre of change.Containing protein and other nutriments in it, there is the title of mycoprotein, containing big
Measure mycelium.Mushroom mycelium residuum and digested through edible mushroom, the compound of the compositions such as crude fibre of qualitative change occurs for structure.Mushroom is
The big kind of China edible mushroom first, China produces more than 700 ten thousand tons of mushroom, about 500,000 tons of mushroom mushroom bran total amount per year（Dry product）More than, it is fragrant
The main matrix of mushroom cultivation is a variety of pesticide forest product leftovers, and these matrix pass through the fermentation of multiple-microorganism, cellulose,
Hemicellulose and wooden grade are by different degrees of degraded, and crude protein, crude fat without the matrix of everfermentation than improving one
More than times, crude fibre reduction by 50%, lignin reduction by 30%.Amino acid contained in mushroom mushroom bran, mushroom polysaccharide and Fe, Ca,
The trace element such as Zn, Mg all relatively enriches.Fraction mushroom mushroom bran is burned as fuel, is abandoned mostly as waste, this is not
Only it is a kind of waste of resource, but also harmful microorganism can be grown, certain pollution is caused to environment.
Raw material will be wanted based on mushroom mushroom bran, carried out after oblique top bacterium is inoculated with after microbial fermentation technology processing, just can be into
For bio-bacterial manure.Changing bacterial manure has obvious nitrogen fixation, and fumaric acid of its mycelia secretion etc. has obvious thorn to plant growth
Swash effect.Not only there are obvious economic benefits, also with obvious ecological and environmental protection effect.This bio-bacterial manure is manured into soil
Afterwards, it can also further improve the physicochemical property of soil, increase soil organic matter content, promote agron and granule group
Formation and conversion, improve soil water-retaining performance and soil fertility, promote crops erosion resistance and volume increase.It can reduce simultaneously
Being excessively used for chemical fertilizer causes many negative effects, such as soil property pollution, environmental pollution.This bio-bacterial manure is directly used in farming
Thing, vegetables production can not only improve yield, and the agricultural product of production are non-harmful product.
At present due to chemical fertilizer fertilizer is used for a long time, cause agricultural production a series of problems occur, add Chinese arable land multiple cropping
Index is high and continuous cropping is produced, and soil continuous production ability, utilization rate of fertilizer decline, soil quality, Quality Safety Problems of Agricultural Products
It is increasingly serious, and these problems are solved, it be unable to do without bio-bacterial manure.
Bio-bacterial manure is while fast development, and its technological innovation is in urgent need to be improved.New function strain, scientific and reasonable work
Skill, the raising of product quality, the reduction of production cost, stabilization of application effect etc., are still that China's bio-feritlizer industry faces
Problem.
The content of the invention
The technical problems to be solved by the invention are：One kind is provided and prepares bio-bacterial manure using mushroom mushroom bran using oblique top bacterium
Method, the disadvantage such as chemical fertilizer soil property pollution, environmental pollution serious unstable for the bio-feritlizer effect that solves to use at present
The technical problem at end.
One kind of the present invention makees matrix with mushroom mushroom bran using oblique top bacterium and prepares bio-bacterial manure, it is characterized in that：
Oblique top bacterium strain is added thereto using mushroom mushroom bran as material to be made as ferment-fermented, strain access amount is
20%, by the way of Liquid solid Bonding ferments, containing organic matter more than 65%, nitrogen, phosphorus, potassium more than 8%, while every gram of bio-bacterial manure
Contain：Nitrogen-fixing bacteria 2.8 ~ 4.5 hundred million, living bacteria count >=1.2 hundred million/gram.
A kind of utilization oblique top bacterium of the present invention makees the method that matrix prepares bio-bacterial manure with mushroom mushroom bran, it is characterized in that
Comprise the following steps：
1st, the oblique top bacterium inclined-plane parent species of artificial culture are chosen（CL-c）Shake-flask seed culture is carried out, culture medium prescription used is：
Peptone 15g, yeast extract 1g, maltose 10g, glucose 10g, wheat bran 50g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 0.75g, dimension
Raw element B1 0.01g, 1000 milliliters of water, pH value 6.5；Above-mentioned raw materials are claimed to be dissolved in water, wheat bran adds water digestion, 30 ~ 40 points under warm fire
Clock, filters to take filtered juice, fully mixes rear high pressure steam sterilization thoroughly（1kg/㎝2）30 minutes, after cooling aseptically, by inclined-plane
It is 1 with the ratio between shaking flask：6（Inclined-plane：Shaking flask）Ratio inoculation, an inclined-plane parent species can connect 2 ~ 3 bottles of 500ml triangular flasks；Take one
Wound on the iron hoop of belt length handle, ring after gauze, suction 95% alcohol, point excessive internal heat rear enclosure forms fire together on big triangular flask bottleneck
Circle, prevents miscellaneous bacteria from invading, and inclined-plane parent species are dug after block, is accessed through pyrosphere center in bottle；26 ~ 25 DEG C of cultivation temperature, rotatably shakes
The rotating speed of bed is 170 ~ 200 revs/min；130 ~ 160 revs/min of the rotating speed early stage of Clothoid type shaking table, 160 revs/min of later stage, training
Support 9 ~ 10 days termination time；
2nd, seed tank culture：Used medium formula is：Add 2000 grams of potato starch, 2000 grams of wheat bran, grape in 1000 liters of tanks
4000 grams of sugar, 1000 grams of beancake powder, 8000 grams of brown sugar, 500 grams of yeast extract, 800 grams of potassium dihydrogen phosphate, 800 grams of magnesium sulfate, dimension life
Plain B1800 pieces, 150 milliliters -200 milliliters of defoamer, substantive 700 liters of amount of solution；
PH value 5.0 ~ 6.0 in fermentation；Steam pressure reaches 0.13Mpa~0.15Mpa in incubator, and culture medium temperature reaches 123
DEG C~126 DEG C, retention time 45min, sterilizing, which terminates cold water being passed through in incubator interlayer or built-in cooling system, to be circulated, and is made
Culture medium is cooled to 24 DEG C~28 DEG C；Inocalation method is protected using flame, pyrosphere is placed on above dog-house and lighted, is now opened
Lid is fed, strain is put into tank rapidly, then, feeding lid is closed rapidly；Flame ring is removed, inoculation is finished；Liquid
Spawn incubation condition：Ventilation is adjusted, is adjusted ventilation to 1 by intake valve and air bleeding valve：0.5V/V ~ 1.2V/V (feed liquids
Volume/volume of air)；Tank pressure is adjusted, regulation tank pressure is maintained between 0.02Mpa~0.04Mpa.Cultivation temperature is adjusted at 24 DEG C
In the range of~25 DEG C；Culture 4d ~ 5d, which can reach, under these conditions puts tank index；
3rd, mushroom mushroom bran and feces of livestock and poultry press 4:1 ratio is mixed as fermentation materials, and left and right compound per ton adds 1 kilogram of side
Spore mold fermentation agent, the moisture of fermentation materials should be controlled 60~65%；
4th, heap fermentation is built：When doing heap, the trapezoidal stack height of flat-top is in 1.2 ~ 1.5 meters or 1.5~2 meters of peaked conical shape heap, width 2
~2.5 meters, length is more than 2~4 meters, and temperature control is at 15 DEG C with top fermentation；In fermentation process, temperature rise to 60~70 DEG C or
To be overturned in time 2~3 times during the above, heating control is at 70 DEG C or so, typically after solid accumulation 48 hours, temperature rises to 60~
70 DEG C, up to more than 70 DEG C, to overturn once at these elevated temperatures within the 3rd day；More than 70 DEG C twice of height occurs in fermentation process
Temperature, the completion that can be fermented in fermentation, normal two weeks can both be completed by overturning 2~3 times；Material is in dark brown, and temperature starts to be down to normal temperature,
Show that fermentation is completed；
5th, the material fermented is poured into fermented and cultured pond, cultured oblique top bacteria liquid strain in access step 2, inoculation
Measure as 20%, 24~25 DEG C of fermented and cultured pond keeping temperature, air blow and agitation, half-light culture, through 15 days or so, material presented a large amount of
White hypha body, i.e. fermented and cultured terminate；
6th, granulate：Granulated using the special prilling process of the organic fertilizer of new high-efficiency, the good material of fermented and cultured is put into system
Grain former, grain products without obvious mechanical admixture, it is uniform in size, without stale flavor, it is prepared by pelletizing forming, then bio-bacterial manure
Finish.
The oblique top bacterium bacterium strain of artificial culture（CL-c）Bacterial strain does not jeopardize human health in enforcement of regulations, and dynamic plant is not carried
Thing cause of disease, it is free from environmental pollution.Through examination, the fly agaric strain of artificial culture（Am-A）Pass on 18 more than generation not degenerate, belong to anti-
Aging ability, stability and metabolic capability are stronger, yield and the higher strain of yield.
The positive effect of the present invention is：
The bio-bacterial manure technique made there is provided oblique top bacterium through solid fermentation, and with matrix is wanted based on mushroom mushroom bran, fostering and apply fertilizer ground
Power, suppress crops to nitrate nitrogen, heavy metal, agricultural chemicals absorption, purification and rehabilitating soil, reduce corps diseases, rush
Enter the utilization of Lenlinus edodes chaff, environmental protection improves the effect of the content of organic matter, and its content of organic matter is up to 65%~80%, can turned into
Good bio-organic fertilizer, not only fertilizer efficiency is good, safe and convenient to use, moreover it is possible to disease-resistant growth-promoting etc..
Embodiment
Although the embodiment of the present invention is the following described, it will be appreciated by those of skill in the art that this is only
It is merely illustrative of, protection scope of the present invention is defined by the appended claims, those skilled in the art is not carrying on the back
From the present invention principle and essence on the premise of, various changes or modifications can be made to these embodiments, these change and
Modification each falls within protection scope of the present invention.
Embodiment 1
Bio-bacterial manure is prepared using oblique top bacterium using mushroom mushroom bran to comprise the following steps：
1st, the oblique top bacterium inclined-plane parent species of artificial culture are chosen（CL-c）Shake-flask seed culture is carried out, culture medium prescription used is：
Peptone 15g, yeast extract 1g, maltose 10g, glucose 10g, wheat bran 50g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 0.75g, dimension
Raw element B1 0.01g, 1000 milliliters of water, pH value 6.5（Before disappearing）.Each medicine is accurately weighed by formula ratio, plus a certain amount of water adds
Heat of solution, is settled to ormal weight, and wheat bran adds water digestion, 30~40 minutes under warm fire, takes filtered juice with eight layers of filtered through gauze, fully mixes
It is sub-packed in after even in triangular flask（500ml triangular flasks loading amount is to 200ml）Tampon beyond the Great Wall（Bag gauze at tampon）, overlying brown paper,
Put high-pressure steam sterilizing pan sterilizing（1kg/㎝2）30 minutes, be 1 by the ratio between inclined-plane and shaking flask after cooling aseptically：6
（Inclined-plane：Shaking flask）Ratio inoculation, a general inclined-plane parent species can connect 2~3 bottles of 500ml triangular flasks.Take the iron of a belt length handle
Wound on ring, ring after gauze, suction 95% alcohol, point excessive internal heat rear enclosure is formed together " pyrosphere ", prevented on big triangular flask bottleneck
Miscellaneous bacteria is invaded, and inclined-plane parent species are dug after block, is accessed through " pyrosphere " center in bottle.26~25 °C of cultivation temperature, rotary shaker
Rotating speed is 170~200 revs/min.130~160 revs/min of the rotating speed early stage of Clothoid type shaking table, 160 revs/min of later stage, culture
9~10 days termination time.
2nd, seed tank culture：Used medium formula is：Add 2000 grams of potato starch, 2000 grams of wheat bran, grape in 1000 liters of tanks
4000 grams of sugar, 1000 grams of beancake powder, 8000 grams of brown sugar, 500 grams of yeast extract, 800 grams of potassium dihydrogen phosphate, 800 grams of magnesium sulfate, dimension life
Plain B1800 pieces, 150 milliliters -200 milliliters of defoamer, substantive 700 liters of amount of solution；Optimal pH value 5.0---6.0 in fermentation；Culture
Steam pressure reaches 0.13Mpa~0.15Mpa in device, and culture medium temperature reaches 123 DEG C~126 DEG C, retention time 45min, gone out
Bacterium, which terminates cold water being passed through in incubator interlayer or built-in cooling system, to be circulated, and culture medium is cooled to 24 DEG C~28 DEG C；Profit
Inocalation method is protected with flame, pyrosphere is placed on above dog-house and lighted, charging lid is now opened, strain is put into rapidly tank
In, then, feeding lid is covered rapidly, tightened；Flame ring is removed, inoculation is finished.Liquid Spawn Culture Conditions：Regulation ventilation
Amount, is adjusted ventilation to 1 by intake valve and air bleeding valve：0.5V/V~1.2V/V (material liquid volume/volume of air)；Adjust tank
Pressure, regulation tank pressure is maintained between 0.02Mpa~0.04Mpa.Cultivation temperature is adjusted in the range of 24 DEG C~25 DEG C；In above-mentioned bar
Culture 4d~5d, which can reach, under part puts tank index；
3rd, mushroom mushroom bran and feces of livestock and poultry press 4:1 ratio is mixed as fermentation materials, and left and right compound per ton adds 1 kilogram of side
Spore mold fermentation agent, the moisture of fermentation materials should be controlled 60~65%；
4th, heap fermentation is built.When doing heap, the trapezoidal stack height of flat-top is at 1.2~1.5 meters（1.5~2 meters of peaked conical shape heap）, width 2
~2.5 meters, length is more than 2~4 meters, and temperature control is at 15 DEG C with top fermentation.Fermentation process notes appropriate oxygen supply and turning（Temperature
Degree will be overturned in time when rising to 60~70 DEG C or more, generally require overturning 2~3 times）, heating control is at 70 DEG C or so, typically
After solid accumulation 48 hours, temperature rises to 60~70 DEG C, up to more than 70 DEG C, to overturn once at these elevated temperatures within the 3rd day,
Generally, more than 70 DEG C twice of high temperature occurs in fermentation process, fermentation, normal two weeks can both be completed by overturning 2~3 times
Interior completion of fermenting.Material is in dark brown, and temperature starts to be down to normal temperature, shows that fermentation is completed；
5th, the material fermented is poured into fermented and cultured pond, cultured oblique top bacteria liquid strain in access step 2, inoculation
Measure as 20%, 24~25 DEG C of fermented and cultured pond keeping temperature, air blow and agitation, half-light culture, through 15 days or so, material presented a large amount of
White hypha body, i.e. fermented and cultured terminate；
6th, granulate：Granulated using the special prilling process of the organic fertilizer of new high-efficiency, the good material of fermented and cultured is put into system
Grain former, grain products without obvious mechanical admixture, it is uniform in size, without stale flavor, it is prepared by pelletizing forming, then bio-bacterial manure
Finish.
Test example 1
Rice application bio-bacterial manure effect test of the present invention：
Test material and method
Material to be tested
For examination fertilizer oblique top bacterium bio-bacterial manure（K2O≤8% of p2o5 containing N, organic matter >=65%, living bacteria count >=1.2 hundred million/
Gram）；Sa Kefu board compound fertilizer dedicated to paddy material 12-16-18, total nutrient >=46%, the limited public affairs of Jilin Province's Long Yuan capital goods in the agricultural sector
Department provides；Good result board compound fertilizer dedicated to paddy material, N- P2O5-K2O 20-13-12 total nutrient >=46%, Jilin Province's good result fertilizer industry
Co., Ltd；
Experimental cultivar length white 24（Rice research institute of academy of agricultural sciences of Jilin Province）2010005（The careful rice of Ji）；
Jilin Province Jiaohe City Tian Gang towns are located at for examination place, experimental plot soil species is miscellaneous husky mud meat field（B5-2）, arable layer relatively deep 22
㎝, medium loam, without obvious barrier factors, irrigation and drainage are convenient；Soil physical and chemical property is before experiment：Soil pH value 6.2, organic matter 27.8
G/kg, the full g/kg of nitrogen 1.59, rapid available phosphorus 44.6mg/kg, the mg/kg of available potassium 99, the mg/kg of slowly available potassium 218, soil fertility is
In on.
Test method
This experiment of experimental design sets four processing, three repetitions.Arranged using random district's groups, test process content：Handle 1 oblique
Push up bacterium bio-bacterial manure；Processing 2 is spread can rich board compound fertilizer dedicated to paddy material；Handle 3 good result board compound fertilizer dedicated to paddy material, processing 4
Do not apply fertilizer；6 × 5=30 square metres of plot area, per cell 30 row, the every root and stem of certain plants of row 30, per the seedling of the root and stem of certain plants two；
Site preparation and transplanting time：Time of site preparation：On May 15th, 2014；Transplanting time：On May 23rd, 2014, using artificial hand
Insert；
Fertilizer amount and fertilizing method：
（1）, basal dressing on May 21st, 2014：Oblique top bacterium bio-bacterial manure area：2.2 kilograms of oblique top bacterium bio-bacterial manure is applied per cell；Spread
Can rich board compound fertilizer dedicated to paddy material area：Every 0.68 kilogram of cell Sa Kefu board compound fertilizer dedicated to paddy material；Good result board paddy rice is special
With the fertile area of compound fertilizer：Any fertilizer is not applied in clear area；
（2）, on June 5th, 2014 apply tillering fertilizer：1.8 kilograms of oblique top bacterium bio-bacterial manure is applied per cell；Sa Kefu board paddy rice is special multiple
Hefei material area：Every 0.66 kilogram of cell Sa Kefu board compound fertilizer dedicated to paddy material；Good result board compound fertilizer dedicated to paddy Liao Fei areas：It is empty
Any fertilizer is not applied in white area；
（3）, on June 29th, 2014 apply ear differentiation fertilizer：1.2 kilograms of oblique top bacterium bio-bacterial manure is applied per cell；Sa Kefu board paddy rice
Special complex fertilizer area：Every 0.5 kilogram of cell Sa Kefu board compound fertilizer dedicated to paddy material；Good result board compound fertilizer dedicated to paddy material fertilizer
Area：Any fertilizer is not applied in clear area.Field management is consistent with Production of Large Fields cultivation management.
Result of the test shows oblique top bacterium bio-bacterial manure area and Sa Kefu boards compound fertilizer dedicated to paddy material area and good result board paddy rice
Special complex fertilizer area processing compare, on paddy rice apply oblique top bacterium bio-bacterial manure when tillering stage, panicle primordium dif ferentiation stage, heading stage,
All faster than other check plots 2-3 days, this explanation can promote Root Growth of Rice, well developed root system, leaf using the bio-bacterial manure of oblique top bacterium
Color is dark green, growing way is vigorous and, blue or green branch cured stalk slower ripe 1-2 days than other check plots in the maturity period, extends aging, is conducive to supporting
Divide accumulation, so as to improve setting percentage；It is right all than other on plant height, the effective fringe of mu, total grain number, bear fruit grains, setting percentage, mass of 1000 kernel
It is high according to area, it is overall more with the obvious advantage than other control fertilization areas using the bio-bacterial manure of oblique top bacterium on paddy rice；
Experiment finishes rear soil sampling laboratory values and is：Soil pH value 5.9, organic matter 36.8 g/kg, the full g/kg of nitrogen 1.96, rapid available phosphorus
50.2mg/kg, the mg/kg of available potassium 126, the mg/kg of slowly available potassium 241；
Oblique top bacterium bio-bacterial manure is applied on paddy rice, the growth of paddy rice can be obviously promoted, special early stage can promote root growth and
The later stage cured stalk of green grass or young crops branch, can extend rice maturity, be conducive to nutrient accumulation, improve setting percentage；Increase rice yield and the output value, prevent
Lodging is notable, disease-resistant, high-output stress-resistance, improves rice quality.Yield increases by 21.68% respectively than other check plots, 23.09%,
90.43%；From the point of view of finishing soil sampling result of laboratory test according to experiment, each available nutrient all increases in soil.Therefore, paddy rice makes
With oblique top bacterium bio-bacterial manure, improve soil physical chemistry effect preferable.
Test example 2
Yield-increasing Baallus In Maize bio-bacterial manure effect test
Kind：The lucky corn variety of agricultural university 823 is quoted from Jilin Agriculture University
Test site：Jilin Province Nongan County Qian Gang townshiies
Test period：In November, -2015 in April, 2015
It is soil for examination soil, its nutrient property is as follows：Organic matter 24.6mg/kg, alkali-hydrolyzable nitrogen 146.8mg/kg, rapid available phosphorus
17.8mg/kg, available potassium 149.3mg/kg, pH value 6.8；
For examination fertilizer：Oblique top bacterium bio-bacterial manure（K2O≤8% of p2o5 containing N, organic matter >=65%, living bacteria count >=1.2 hundred million/
Gram）.Other fertilizer have urea（Nitrogenous 46%）, Diammonium phosphate (DAP)（Phosphorus pentoxide content 46%, nitrogen content 18%）, potassium sulfate（Oxidation
Potassium content 40%）；
Experimental design：Plot experiment is taken in this experiment, if 4 processing, 3 repetitions, altogether 12 experimental plots, each cell face
31,2 square metres of product.Each cell random alignment；
Processing 1：Reduce 10% dose than local conventional fertilizer application amount, while mu makees base fertilizer for 10 kilograms using bio-bacterial manure, disposably
Apply；
Processing 2：Reduce 10% dose than local conventional fertilizer application amount, while mu makees base fertilizer using 10 kilograms of the bio-bacterial manure of inactivation,
It is disposable to apply；
Processing 3：Conventional fertilizer application；
Processing 4：Blank（Without using any fertilizer）；
Fertilizing method：Conventional fertilizer application, mu applies Chemical Mixed Fertilizer（14-18-15）36 kilograms are made base fertilizer, disposable to apply；
Result of the test：
Influence using bio-bacterial manure to corn growth, result of the test shows, processing of the corn using bio-bacterial manure and its
He handles and compared, and well developed root system, growing way is good, and effect of increasing production is obvious；
Influence using bio-bacterial manure to corn yield：1 average product is handled for 31.5kg, kg/ mus of per mu yield 647 is converted into；Place
2 average products are managed for 29..3kg, 602.5kg/ mus of per mu yield is converted into；3 average products are handled for 29kg, per mu yield 594 is converted into
Kg/ mus；4 average products are handled for 17.4kg, kg/ mus of per mu yield 355 is converted into；
Conclusion (of pressure testing)：Corn increases production 44.5 kg/ mus using bio-bacterial manure of the processing than inactivation of bio-bacterial manure, and rate of growth is
7.4%, than 53 kg/ mus of conventional fertilizer application processing volume increase, rate of growth increases production 292kg/ mus, rate of growth reaches up to 8.9% than blank control
82.4%。
Test example 3
Soybean applies bio-bacterial manure effect test
Kind：Ji educates 506, quoted from Inst. of Soybean, Jilin Academy of Agriculture.For examination place, Dunhua of Jilin Province city land owned by officials town.
Soybean is the main cultivation crop of east of Jilin province, and cultivated area is big, and stubble plantation is met again generally, causes pest and disease damage to be endangered
Evil, trophic disturbance have impact on the yield and quality of soybean, therefore, having carried out field plot trial research using bio-bacterial manure；
Materials and methods
Fertilizer：Oblique top bacterium bio-bacterial manure（K2O≤8% of p2o5 containing N, organic matter >=65%, living bacteria count >=1.2 hundred million/gram）.Phosphorus
Sour two ammoniums, urea（Bought by local means of production company）；
Method：Experimental plot is selected in second crop soil, and medium loam, the plot experiments of medium fertility set three processing：It is 1 phosphorus of processing respectively
Sour two ammoniums:4.5kg, urea 2.6kg, bio-bacterial manure 3.5kg；Handle 2 Diammonium phosphate (DAP) 4.5kg+ bio-bacterial manures 5.5kg；Handle 3 phosphorus
Two ammonium 12kg/667m of acid2；
By the dose of design, sowed using two-row seeder；The biological character of soybean seedling is investigated, pest and disease damage is endangered
Production species test is surveyed before evil, harvesting.As a result statistical analysis is carried out；
Influence of the bio-bacterial manure to Soybean Growth Characters process：Each processing carries out field respectively at soybean seeding stage, early flowering season and pod phase beginning
Between investigate, seeding stage, the early flowering season equal indifference respectively handled, and handle 2 and 3 shift to an earlier date 1 day in pod phase beginning than processing 1 and processing.Begin
Florescence root fresh weight processing 1 is than processing gram/plant of 3 mean height 0.55.1 disease index is handled from root rot from the point of view of early flowering season incidence
It is lower by 2.35% than compareing.Other diseases are not occurring experimental field.Plant weights and plant fresh weight have been investigated simultaneously in the early flowering season,
Processing 1 significantly increases plant weights, and 16.8% is added than control；Plant fresh weight adds 2.2%.As a result bio-fertilizer is shown
Administration can promote the root system development of soybean, significantly increase the biological yield of plant；
Influence of the bio-bacterial manure to soybean yield：The plant height of processing 1 and processing 2 is higher 2.8-3.4 centimetres than compareing, place
Reason 1 and the 100-grain weight of processing 2 improve 0.35-0.51 grams compared with the control, and effective pod number is than control increase 0.95-1.2, strain grain
Number is than control increase 1.6-2.4.Each processing has different degrees of volume increase than control, wherein handling 1 yield highest.Handle 1 He
Diammonium phosphate (DAP) volume increase 6.83% and 48.6% is more singly applied in processing 2 respectively；
Use processing 1 and the He of increase by 27.84% of soybean seedling underground part radical more single ammonium of application of phosphoric acid two respectively of processing 2
38.9%;Root length increases by 17.8% and 6.6% respectively;Root nodule number increases by 28.5% and 53.2% respectively；
Influence of the bio-bacterial manure to soybean diseases and insect pests
To the prevention effect of soybean seedling root rot after influence application processing 1 and 2 fertilizer of processing of the bio-bacterial manure to soybean diseases
Respectively 26.5% and 58.9%；Prevention effect to soy bean cyst roundworm is respectively 59.6% and 69.3%；To purple spot of soybean
Prevention effect is respectively 34.5% and 5.8%；
Influence of the bio-bacterial manure to soybean insect pest：
Using the prevention effect of Ophiomyia shibatsuji after processing 1 and processing 2 up to 100%；White edge cutworm larva after the processing of processing 2
Generating capacity more singly apply Diammonium phosphate (DAP) have dropped 49.8%, processing 1 is little compared with Diammonium phosphate (DAP) difference is singly applied；
Bio-bacterial manure has preferable production-increasing function processing 1 and the processing 2 relatively volume increase of processing 3 6.83% and 48.6% respectively.The original of volume increase
Because being that fertilizer has certain facilitation to growing for soybean seedling, radical and the increase of root nodule number of underground are shown, is planted
The increase of plant weight amount, makes the later stage bear pods and counts increase.Bio-bacterial manure has certain inhibitory action to handle 1 and place to soybean seedling pest and disease damage
The harm mitigation of 2 soybean cyst nematodes, root rot, root-miner is managed, bio-fertilizer can suppress harmful bacteria, significantly reduce soybean
Root rot morbidity occurs, and provides good soil environment for the growth of soybean, Pod Bearing Percentage and 100-grain weight is improved, so as to reach
The purpose of volume increase.
Claims (2)
1. one kind makees matrix using oblique top bacterium with mushroom mushroom bran prepares bio-bacterial manure, it is characterized in that being used as material using mushroom mushroom bran
Add oblique top bacterium strain thereto to be made as ferment-fermented, strain access amount is 20%, the side fermented using Liquid solid Bonding
Formula, containing organic matter more than 65%, nitrogen, phosphorus, potassium more than 8%, while every gram of bio-bacterial manure contains：Nitrogen-fixing bacteria 2.8 ~ 4.5 hundred million, have
Effect viable count >=1.2 hundred million/gram.
2. a kind of utilization oblique top bacterium makees the method that matrix prepares bio-bacterial manure with mushroom mushroom bran, it is characterized in that comprising the following steps：
1）Choose the oblique top bacterium inclined-plane parent species of artificial culture（CL-c）Shake-flask seed culture is carried out, culture medium prescription used is：
Peptone 15g, yeast extract 1g, maltose 10g, glucose 10g, wheat bran 50g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 0.75g, dimension
Raw element B1 0.01g, 1000 milliliters of water, pH value 6.5；Above-mentioned raw materials are claimed to be dissolved in water, wheat bran adds water digestion, 30 ~ 40 points under warm fire
Clock, filters to take filtered juice, fully mixes rear high pressure steam sterilization thoroughly（1kg/㎝2）30 minutes, after cooling aseptically, by inclined-plane
It is 1 with the ratio between shaking flask：6（Inclined-plane：Shaking flask）Ratio inoculation, an inclined-plane parent species can connect 2 ~ 3 bottles of 500ml triangular flasks；Take one
Wound on the iron hoop of belt length handle, ring after gauze, suction 95% alcohol, point excessive internal heat rear enclosure forms fire together on big triangular flask bottleneck
Circle, prevents miscellaneous bacteria from invading, and inclined-plane parent species are dug after block, is accessed through pyrosphere center in bottle；26 ~ 25 DEG C of cultivation temperature, rotatably shakes
The rotating speed of bed is 170 ~ 200 revs/min；130 ~ 160 revs/min of the rotating speed early stage of Clothoid type shaking table, 160 revs/min of later stage, training
Support 9 ~ 10 days termination time；
2）Seed tank culture：Used medium formula is：Add 2000 grams of potato starch, 2000 grams of wheat bran, grape in 1000 liters of tanks
4000 grams of sugar, 1000 grams of beancake powder, 8000 grams of brown sugar, 500 grams of yeast extract, 800 grams of potassium dihydrogen phosphate, 800 grams of magnesium sulfate, dimension life
Plain B1800 pieces, 150 milliliters -200 milliliters of defoamer, substantive 700 liters of amount of solution；
PH value 5.0 ~ 6.0 in fermentation；Steam pressure reaches 0.13Mpa~0.15Mpa in incubator, and culture medium temperature reaches 123
DEG C~126 DEG C, retention time 45min, sterilizing, which terminates cold water being passed through in incubator interlayer or built-in cooling system, to be circulated, and is made
Culture medium is cooled to 24 DEG C~28 DEG C；Inocalation method is protected using flame, pyrosphere is placed on above dog-house and lighted, is now opened
Lid is fed, strain is put into tank rapidly, then, feeding lid is closed rapidly；Flame ring is removed, inoculation is finished；Liquid
Spawn incubation condition：Ventilation is adjusted, is adjusted ventilation to 1 by intake valve and air bleeding valve：0.5V/V ~ 1.2V/V (feed liquids
Volume/volume of air)；Tank pressure is adjusted, regulation tank pressure is maintained between 0.02Mpa~0.04Mpa；Cultivation temperature is adjusted at 24 DEG C
In the range of~25 DEG C；Culture 4d ~ 5d, which can reach, under these conditions puts tank index；
3）Mushroom mushroom bran presses 4 with feces of livestock and poultry:1 ratio is mixed as fermentation materials, and left and right compound per ton adds 1 kilogram of side
Spore mold fermentation agent, the moisture of fermentation materials should be controlled 60~65%；
4）Build heap fermentation：When doing heap, the trapezoidal stack height of flat-top is in 1.2 ~ 1.5 meters or 1.5~2 meters of peaked conical shape heap, width 2
~2.5 meters, length is more than 2~4 meters, and temperature control is at 15 DEG C with top fermentation；In fermentation process, temperature rise to 60~70 DEG C or
To be overturned in time 2~3 times during the above, heating control is at 70 DEG C or so, typically after solid accumulation 48 hours, temperature rises to 60~
70 DEG C, up to more than 70 DEG C, to overturn once at these elevated temperatures within the 3rd day；More than 70 DEG C twice of height occurs in fermentation process
Temperature, the completion that can be fermented in fermentation, normal two weeks can both be completed by overturning 2~3 times；Material is in dark brown, and temperature starts to be down to normal temperature,
Show that fermentation is completed；
5）The material fermented is poured into fermented and cultured pond, cultured oblique top bacteria liquid strain in access step 2, inoculation
Measure as 20%, 24~25 DEG C of fermented and cultured pond keeping temperature, air blow and agitation, half-light culture, through 15 days or so, material presented a large amount of
White hypha body, i.e. fermented and cultured terminate；
6）Granulation：Granulated using the special prilling process of the organic fertilizer of new high-efficiency, the good material of fermented and cultured is put into system
Grain former, grain products without obvious mechanical admixture, it is uniform in size, without stale flavor, it is prepared by pelletizing forming, then bio-bacterial manure
Finish.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN108901600A (en) * | 2018-08-09 | 2018-11-30 | 合肥福泉现代农业科技有限公司 | A kind of method that expired milk recycles cultivation milk fragrance edible mushroom |
| CN115947630A (en) * | 2023-02-09 | 2023-04-11 | 江苏东来生物科技有限公司 | A kind of solid fermentation method of biological fertilizer |
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