CN107132216A - A kind of breeding water body ammonia nitrogen quick detection kit - Google Patents

Abstract

The invention discloses a kind of breeding water body ammonia nitrogen quick detection kit.Contain ammonia nitrogen test agent I, ammonia nitrogen test agent II, colorimetric bottle and colorimetric card in the kit；Described test agent includes p-aminobenzene sulfonic acid, hydrochloride naphthodiamide, acids, surfactant, stabilizer and distilled water；Many advantages, such as kit has rapid sensitive, easy to use, steady quality, it not only compensate for conventional detection kit complex operation step or the problems such as the rotten discoloration of reagent caused by after a long time in detection process, operating procedure is simply efficient simultaneously, additional any equipment is not needed, it is adaptable to the field quick detection of multiple-quality water.

Description

A rapid detection kit for ammonia nitrogen in aquaculture water

technical field

The invention belongs to the field of water body detection, and in particular relates to a rapid detection kit for ammonia nitrogen in aquaculture water.

Background technique

The ammonia nitrogen content in aquaculture water is an important indicator of water quality testing in the aquaculture process. Ammonia nitrogen (NH ₃ -N) in water mainly exists in the form of free ammonia (NH ₃ ) or ammonium salt (NH ₄ ⁺ ); The ammonia nitrogen in the water body is mainly produced by the excrement of farmed animals decomposed by microorganisms in the water body, the organic fertilizer in the water body, and residual bait; Nitrate, etc. are decomposed into ammonia nitrogen. If the content of ammonia nitrogen in the water body is too high, it will cause damage to the body tissues of farmed animals and reduce the transport of oxygen between tissues; it will increase the permeability of fish gills, which is not conducive to its normal substance exchange with the outside world; it will affect the health of farmed organisms Feeding efficiency leads to slow production, decreased immunity and easy disease, which will lead to the continuous accumulation of toxic substances in the water body, directly endangering the growth and survival of farmed animals, and will cause poisoning death in severe cases.

In the process of detecting water samples, the existing ammonia nitrogen detection kits on the market sometimes react with reagents due to excessive metal ions such as calcium ions, magnesium ions, iron ions, and manganese ions in the water samples to form white precipitates or turbidity. , so that the color cannot be developed; it seriously affects the detection of ammonia nitrogen content in water. Commonly used surfactants such as potassium sodium tartrate that are used to mask these metal ions cannot completely and completely mask the metal ions in water samples due to various reasons such as quality; or the quality of chemical reagents due to the impurity of mercuric chloride reagent The change affects the color observation during the detection of ammonia nitrogen.

Therefore, with the rapid development of the aquaculture industry, to reduce the breeding risk of farmers, there is an urgent need for a new fast, accurate and stable ammonia nitrogen detection reagent, which is convenient for farmers to quickly and accurately judge the ammonia nitrogen content in the aquaculture water. .

Contents of the invention

In order to solve the above problems, the present invention provides a rapid detection kit for ammonia nitrogen in aquaculture water.

The rapid detection kit for ammonia nitrogen in aquaculture water provided by the present invention contains ammonia nitrogen test agent I, ammonia nitrogen test agent II, a colorimetric bottle and a color card, and the ammonia nitrogen test agent I is composed of potassium sodium tartrate 25- 60 parts, 10-45 parts of surfactant, 1-30 parts of stabilizer, 75-120 parts of distilled water; the ammonia nitrogen test reagent II is made by parts by weight: 5-35 parts of sodium hydroxide, potassium mercury iodide 5-25 parts, 5-35 parts of stabilizer, 75-120 parts of distilled water.

Preferably, the ammonia nitrogen test reagent I is made by parts by weight: 28-55 parts of potassium sodium tartrate, 12-40 parts of surfactant, 3-25 parts of stabilizer, and 80-110 parts of distilled water; the ammonia nitrogen The test agent II is prepared by parts by weight: 8-32 parts of sodium hydroxide, 8-24 parts of mercury potassium iodide, 8-30 parts of stabilizer and 80-110 parts of distilled water.

More preferably, the ammonia nitrogen test reagent I is made by parts by weight: 30-50 parts of potassium sodium tartrate, 15-35 parts of surfactant, 5-24 parts of stabilizer, and 85-105 parts of distilled water; The ammonia nitrogen test reagent II is prepared by parts by weight: 10-30 parts of sodium hydroxide, 10-20 parts of potassium mercury iodide, 10-25 parts of stabilizer and 85-105 parts of distilled water.

Most preferably, the ammonia nitrogen test reagent I is made by parts by weight: 35-45 parts of potassium sodium tartrate, 20-30 parts of surfactant, 10-20 parts of stabilizer, and 90-100 parts of distilled water; Ammonia nitrogen test reagent II is prepared by parts by weight: 12-25 parts of sodium hydroxide, 12-18 parts of potassium mercury iodide, 12-20 parts of stabilizer and 90-100 parts of distilled water.

Wherein, the volume ratio of the ammonia nitrogen test reagent I to the nitrogen test reagent II is preferably 1:1.

Wherein, the stabilizer is one or more of disodium edetate, monoethanolamine, triethanolamine, polyvinyl alcohol, and thiourea.

Wherein, the surfactant is one or more of sodium hexametaphosphate, sodium acetate, sodium malate, and sodium citrate.

In the present invention, the colorimetric card adopts the spectrophotometer to measure (3 parallels) calibration respectively by the ammonia nitrogen standard solution (0.005, 0.01, 0.05, 0.10, 0.15, 0.20, 0.25, 0.30mg/L) of different concentration gradients of configuration Finally, the standard solution is produced by developing color with the test reagent.

The present invention also provides a method for detecting ammonia nitrogen in aquaculture water using the rapid detection kit for ammonia nitrogen in aquaculture water of the present invention, which includes the following steps:

1) Use the water sample to be tested to rinse the colorimetric bottle 2 to 3 times;

2) Use a straw to draw the water sample to be tested into the colorimetric bottle, and set the volume to 15mL.

3) Add 6 drops each of ammonia nitrogen test reagent Ⅰ and ammonia nitrogen test reagent Ⅱ, cover the color comparison bottle, shake well and let it stand for 1-3 minutes, compare it with the standard color comparison card, and find out the color block with similar color, which is the color in the water sample to be tested. Ammonia nitrogen content.

Advantage and positive effect of the present invention are:

In the novel ammonia nitrogen rapid detection kit provided by the present invention, the test agent is provided in a liquid form, which is convenient to use; other new masking agents added in the test agent I can cooperate with potassium sodium tartrate to mask the metal ions in the water sample, so that the test agent The properties are more stable and easy to store. The chromogenic group potassium mercuric iodide is directly used in the test reagent II, which effectively prevents the formation of red precipitates of mercuric iodide. At the same time, a stabilizer is added to solve the problem of reagent sensitivity reduction caused by unstable test reagent quality. The reagent solves the problem that the water quality becomes turbid in the process of testing water samples after adding the test reagent.

The stabilizer added in the test reagent of the present invention can maintain the stability of the physical and chemical properties of the test reagent and product quality, and increase the effective shelf life of the test reagent. After adding the new test agent, the water sample no longer becomes turbid, and the color develops quickly and clearly. Farmers can quickly and accurately understand the ammonia nitrogen content in the water sample. At the same time, the preparation cost of the test agent of the present invention is low, and the materials are convenient and easy to obtain. The method effectively solves the demand for rapid and accurate detection of ammonia nitrogen in aquaculture water, and has broad application prospects in the field of water quality detection.

detailed description

The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

Example 1

Ammonia nitrogen test reagent Ⅰ includes by weight: 60 parts of potassium sodium tartrate, 30 parts of sodium citrate, 20 parts of disodium edetate, dissolved in 120 parts of distilled water, heated in a water bath at 90°C for 15 minutes, and cooled to obtain .

Ammonia nitrogen test reagent II includes by weight: 35 parts of sodium hydroxide, 25 parts of potassium mercury iodide, and 20 parts of disodium edetate, which are dissolved in 120 parts of distilled water.

Kit assembly: 1 bottle of ammonia nitrogen test reagent Ⅰ, 1 bottle of ammonia nitrogen test reagent Ⅱ, 1 color comparison card, 1 color comparison bottle.

The specific operation steps of ammonia nitrogen detection in aquaculture water are as follows:

(1) Use the water sample to be tested to rinse the colorimetric bottle 2 to 3 times;

(2) Use a straw to draw the water sample to be tested into the colorimetric bottle, and set the volume to 15mL.

(3) Add 6 drops each of ammonia nitrogen test reagent Ⅰ and ammonia nitrogen test reagent Ⅱ, cover the color comparison bottle, shake well and let it stand for 1-3 minutes, compare it with the standard color comparison card, and find out the color block with similar color, which is the water sample to be tested ammonia nitrogen content.

Example 2

Ammonia nitrogen test reagent I includes by weight: 40 parts of sodium potassium tartrate, 30 parts of sodium hexametaphosphate, 15 parts of sodium acetate, 1 part of disodium edetate, dissolved in 75 parts of distilled water, and heated in a water bath at 90°C After 25 minutes, cool and serve.

Ammonia nitrogen test reagent II includes by weight: 15 parts of sodium hydroxide, 25 parts of potassium mercuric iodide, 5 parts of monoethanolamine, and 10 parts of triethanolamine, which are dissolved in 95 parts of distilled water to obtain final product.

Kit assembly: 1 bottle of ammonia nitrogen test reagent Ⅰ, 1 bottle of ammonia nitrogen test reagent Ⅱ, 1 color comparison card, 1 color comparison bottle.

The specific operation steps of ammonia nitrogen detection in aquaculture water are as follows:

(1) Use the water sample to be tested to rinse the colorimetric bottle 2 to 3 times;

(2) Use a straw to draw the water sample to be tested into the colorimetric bottle, and set the volume to 15mL.

(3) Add 6 drops each of ammonia nitrogen test agent Ⅰ and ammonia nitrogen test agent Ⅱ according to the volume ratio, cover the colorimetric bottle, shake it well and let it stand for 1-3 minutes, compare it with the standard colorimetric card, and find the color block with similar color to be tested Ammonia nitrogen content in water samples.

Example 3

Ammonia nitrogen test reagent I includes by weight: 25 parts of potassium sodium tartrate, 30 parts of sodium acetate, 5 parts of sodium malate, 15 parts of disodium edetate, 15 parts of thiourea, dissolved in 95 parts of distilled water, and dissolved in 90 parts ℃ water bath heating for 30min, cooling, that is.

Ammonia nitrogen test reagent II includes by weight: 5 parts of sodium hydroxide, 35 parts of potassium mercury iodide, and 15 parts of polyvinyl alcohol, which are dissolved in 100 parts of distilled water.

Kit assembly: 1 bottle of ammonia nitrogen test reagent Ⅰ, 1 bottle of ammonia nitrogen test reagent Ⅱ, 1 color comparison card, 1 color comparison bottle.

The specific operation steps of ammonia nitrogen detection in aquaculture water are as follows:

(1) Use the water sample to be tested to rinse the colorimetric bottle 2 to 3 times;

(2) Use a straw to draw the water sample to be tested into the colorimetric bottle, and set the volume to 15mL.

(3) Add 6 drops each of ammonia nitrogen test reagent Ⅰ and ammonia nitrogen test reagent Ⅱ, cover the color comparison bottle, shake well and let it stand for 1-3 minutes, compare it with the standard color comparison card, and find out the color block with similar color, which is the water sample to be tested ammonia nitrogen content.

Example 4

Ammonia nitrogen test reagent Ⅰ includes by weight: 50 parts of potassium sodium tartrate, 15 parts of sodium hexametaphosphate, 15 parts of disodium edetate, 15 parts of polyvinyl alcohol, dissolved in 100 parts of distilled water, and placed in a water bath at 90 °C Heating for 30min, cooling, that is.

Ammonia nitrogen test reagent II includes by weight: 24 parts of sodium hydroxide, 5 parts of potassium mercuric iodide, 10 parts of disodium edetate, and 10 parts of polyvinyl alcohol, dissolved in 100 parts of distilled water to obtain the product.

Kit assembly: 1 bottle of ammonia nitrogen test reagent Ⅰ, 1 bottle of ammonia nitrogen test reagent Ⅱ, 1 color comparison card, 1 color comparison bottle.

The specific operation steps are as follows:

(1) Use the water sample to be tested to rinse the colorimetric bottle 2 to 3 times;

(2) Use a straw to draw the water sample to be tested into the colorimetric bottle, and set the volume to 15mL.

(3) Add 0.2 mL of ammonia nitrogen test reagent Ⅰ and ammonia nitrogen test reagent Ⅱ at a volume ratio of 1:1, cover the colorimetric bottle, shake it well and let it stand for 1 to 3 minutes, compare it with the standard color comparison card, and find out the color blocks with similar colors. Ammonia nitrogen content in the water sample to be tested.

Test example 1

The ammonia nitrogen test reagents prepared in Example 1, Example 2, Example 3, and Example 4 of the present invention were used to observe the detection effect of the ammonia nitrogen standard solution with the same concentration gradient.

Prepare the ammonia nitrogen standard solution by a certain concentration gradient, use the detection reagent in the present invention to carry out the ammonia nitrogen content determination in the solution to it respectively, each concentration is all detected three times, carry out visual colorimetry with the colorimetric card corresponding to the kit, The concentration measured by the colorimetric result is consistent with the concentration of the prepared standard solution, indicating that the accuracy of the ammonia nitrogen detection reagent in the present invention meets the requirements.

Table 1 Comparison of the effects of ammonia nitrogen detection reagents prepared by the present invention

The test results show that the detection effect of the ammonia nitrogen detection reagent prepared by 4 embodiments of the present invention is consistent with the concentration of the prepared standard solution, and wherein embodiment 2 has the best effect in terms of reaction speed among the 4 embodiments, showing that the effect of embodiment 2 The surfactant and stabilizer in the ammonia nitrogen test reagent have a synergistic effect in terms of proportion and function, and jointly promote and speed up the reaction.

Test example 2 the test of ammonia nitrogen test agent stability and shelf life of the present invention

Accelerated test:

The ammonia nitrogen test reagent prepared in Example 1 of the present invention was placed in three constant temperature and humidity incubators, the temperatures in the boxes were 4°C, 25°C, and 40°C; the humidity was 75°C, and placed for 2 weeks. 4°C is used to maintain the chemical properties of the test agent, as a control sample; 25°C is used to simulate normal temperature storage samples; 40°C is used for accelerated tests, as environmentally destructive samples. Then the same batch of aquaculture water samples were tested, and the test results were consistent with those at 4°C, 25°C, and 40°C, indicating that the measured values of the ammonia nitrogen test agent prepared in Example 1 were all within the normal color range, and the test agent was stable. .

Adopt the test of the accuracy of the ammonia nitrogen test agent prepared by the embodiment of the present invention 2 and the shelf life:

Long term test:

Store a batch of detection kits prepared at the same time at room temperature in the dark. After 2 months, 4 months, 6 months, 8 months, 10 months, and 12 months, 5 groups of detection kits were randomly selected to measure the ammonia nitrogen content of multiple groups of water samples at the same time, and the time was used at the same time Point the newly prepared reagent to measure the same water sample, and the reagent box stored for a period of time and the newly configured reagent all show consistency in the detection results of each group of water samples. The experimental results show that the ammonia nitrogen prepared in Example 3 of the present invention The test reagents will not deteriorate within one year, and the test results of long-term preserved reagents can also meet the standard requirements.

Table 2 The test results of the ammonia nitrogen detection reagent prepared in Example 2 of the present invention to detect water samples

Experimental results show that the ammonia nitrogen test reagent prepared in Example 2 of the present invention will not deteriorate within one year, and the test results of long-term stored reagents can also meet the standard requirements.

Test example 3

The detection speed effect of the ammonia nitrogen test reagent prepared in Example 3 of the present invention and other ammonia nitrogen detection reagents was compared and observed.

The first group is the ammonia nitrogen test reagent prepared in Example 3, the second group is the ammonia nitrogen detection reagent prepared according to the Chinese patent CN102393329A, the third group is the ammonia nitrogen detection reagent prepared according to the Chinese patent CN102507553A, and the fourth group is the control group using a spectrophotometer To measure the ammonia nitrogen content, the ammonia nitrogen content was detected in the same water sample from a farm in Zhaoan, and the reaction time of the reagent was recorded. The results are shown in Table 3.

Table 3 The reaction time comparison of the ammonia nitrogen test reagent prepared in Example 3 of the present invention to detect water samples

The test results show that the detection accuracy and reaction speed of the ammonia nitrogen detection reagent prepared in Example 3 of the present invention are significantly better than those of the second and third groups of ammonia nitrogen reagents.

Test example 4

The stability of the ammonia nitrogen salt test agent prepared by using Example 4 of the present invention is compared with other ammonia nitrogen test agents for observation of stability.

Stability comparison test of the new ammonia nitrogen detection kit: use the first group of ammonia nitrogen test reagents prepared in Example 4 for the same period of time for 6 months and 12 months, and the second group of ammonia nitrogen detection reagents prepared according to Chinese patent CN102393329A, The three groups were compared with ammonia nitrogen detection reagents prepared according to Chinese patent CN102507553A. The ammonia nitrogen content was detected in the same water sample from a grass carp farm in Dongshan, and the color changes were recorded. The results are shown in Table 4. The color of the ammonia nitrogen test agent prepared in Example 4 of the present invention is consistent with the color of the standard color card, and the water samples detected by the second group become turbid and cannot be compared with the color; the color contrast between the color development result of the third group and the color card has changed; The results show that the ammonia nitrogen detection reagent of the present invention has the same color as the color card, and the stability of the reagent is obviously better than that of the second and third groups of ammonia nitrogen detection reagents.

Table 4 The ammonia nitrogen test reagent prepared in Example 4 of the present invention detects color change test results

The results of this test show that the quality stability and storage time of the ammonia nitrogen test reagent prepared by comparing Example 4 of the present invention are significantly better than the detection effects of the second group and the third group of ammonia nitrogen detection reagents.

The above is only a preferred embodiment of the present invention, it should be pointed out that for those of ordinary skill in the art, without departing from the technical principle of the present invention, some improvements and modifications can also be made. These improvements and modifications It should also be regarded as the protection scope of the present invention.

Claims (8)

1. A rapid detection kit for ammonia nitrogen in aquaculture water, which contains ammonia nitrogen test agent I, ammonia nitrogen test agent II, colorimetric bottle and color card, and described ammonia nitrogen test agent I is by weight: sodium potassium tartrate 25- 60 parts, 10-45 parts of surfactant, 1-30 parts of stabilizer, 75-120 parts of distilled water; the ammonia nitrogen test reagent II is made by parts by weight: 5-35 parts of sodium hydroxide, potassium mercury iodide 5-25 parts, 5-35 parts of stabilizer, 75-120 parts of distilled water. 2. culture water body ammonia nitrogen rapid detection kit according to claim 1, is characterized in that, described ammonia nitrogen test agent 1 is by weight: 28-55 parts of sodium potassium tartrate, 12-40 parts of tensio-active agent, 3-25 parts of stabilizer, 80-110 parts of distilled water; the ammonia nitrogen test reagent II is made up of: 8-32 parts of sodium hydroxide, 8-24 parts of potassium mercuric iodide, and 8-30 parts of stabilizer , made from 80-110 parts of distilled water. 3. culture water body ammonia nitrogen rapid detection kit according to claim 2, is characterized in that, described ammonia nitrogen test agent 1 is by weight: 30-50 parts of sodium potassium tartrate, 15-35 parts of tensio-active agent, 5-24 parts of stabilizer, 85-105 parts of distilled water; the ammonia nitrogen test reagent II is made of: 10-30 parts of sodium hydroxide, 10-20 parts of potassium mercury iodide, and 10-25 parts of stabilizer , made from 85-105 parts of distilled water. 4. culture water body ammonia nitrogen rapid detection kit according to claim 3, is characterized in that, described ammonia nitrogen test agent 1 is by weight: potassium sodium tartrate 35-45 parts, tensio-active agent 20-30 parts, 10-20 parts of stabilizer, 90-100 parts of distilled water; the ammonia nitrogen test reagent II is composed of 12-25 parts of sodium hydroxide, 12-18 parts of potassium mercury iodide, and 12-20 parts of stabilizer , made from 90-100 parts of distilled water. 5. The rapid detection kit for ammonia nitrogen in aquaculture water according to any one of claims 1 to 4, wherein the volume ratio of the ammonia nitrogen test agent I to the nitrogen test agent II is 1:1. 6. according to any one of claims 1 to 4, the rapid detection kit for ammonia nitrogen in aquaculture water, is characterized in that, the stabilizer is disodium edetate, monoethanolamine, triethanolamine, polyvinyl alcohol, One or more of thioureas. 7. according to any one of claims 1 to 4, the rapid detection kit for ammonia nitrogen in aquaculture water, wherein the surfactant is one of sodium hexametaphosphate, sodium acetate, sodium malate, and sodium citrate. one or more species. 8. The method for detecting ammonia nitrogen in aquaculture water using the rapid detection kit for ammonia nitrogen in aquaculture water according to any one of claims 1 to 7, comprising the steps of: 1) Use the water sample to be tested to rinse the colorimetric bottle 2 to 3 times; 2) Use a straw to draw the water sample to be tested into the colorimetric bottle, and set the volume to 15mL. 3) Add 6 drops each of ammonia nitrogen test reagent Ⅰ and ammonia nitrogen test reagent Ⅱ, cover the color comparison bottle, shake well and let it stand for 1-3 minutes, compare it with the standard color comparison card, and find out the color block with similar color, which is the color in the water sample to be tested. Ammonia nitrogen content.