CN106957816A - Lipolytic activity cell transplants instrument automatically - Google Patents
Lipolytic activity cell transplants instrument automatically Download PDFInfo
- Publication number
- CN106957816A CN106957816A CN201710197776.0A CN201710197776A CN106957816A CN 106957816 A CN106957816 A CN 106957816A CN 201710197776 A CN201710197776 A CN 201710197776A CN 106957816 A CN106957816 A CN 106957816A
- Authority
- CN
- China
- Prior art keywords
- lauter tub
- lipolytic activity
- pipeline
- activity cell
- grease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 230000002366 lipolytic effect Effects 0.000 title claims abstract description 22
- 239000004519 grease Substances 0.000 claims abstract description 39
- 230000005540 biological transmission Effects 0.000 claims abstract description 14
- 230000035764 nutrition Effects 0.000 claims description 9
- 235000016709 nutrition Nutrition 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 20
- 238000001556 precipitation Methods 0.000 abstract description 8
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 238000000926 separation method Methods 0.000 abstract description 7
- 238000000605 extraction Methods 0.000 description 10
- 238000001914 filtration Methods 0.000 description 6
- 230000009467 reduction Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0653—Adipocytes; Adipose tissue
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/04—Phase separators; Separation of non fermentable material; Fractionation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
- C12N2509/10—Mechanical dissociation
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Sustainable Development (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Rheumatology (AREA)
- Computer Hardware Design (AREA)
- Cell Biology (AREA)
- Mechanical Engineering (AREA)
- Fats And Perfumes (AREA)
Abstract
Instrument is transplanted automatically the invention discloses a kind of lipolytic activity cell, include instrument body, the first lauter tub and the second lauter tub are provided with inside the instrument body, realized and connected by transmission pipeline between first lauter tub and the second lauter tub, the outside of first lauter tub is provided with funnel type drainage part, and the transmission pipeline is passed through on the side wall of the funnel type drainage part, the top of first lauter tub is provided with grease inlet, the second lauter tub bottom is provided with out fat pipeline, drive shaft is provided with second lauter tub, cutter are distributed with the drive shaft, the drive shaft has motor driving；The separation of grease and water is realized by the first lauter tub and the second lauter tub, is realized using cutter and the grease of precipitation is smashed, using acquisition of the fat pipeline realization to grease competent cell is gone out, the purpose for the work load for improving production efficiency and reducing public personnel is reached.
Description
Technical field
The present invention relates to fat separation acquisition field, and in particular to lipolytic activity cell transplants instrument automatically.
Background technology
Generally obtained in the acquisition of existing grease competent cell using manual type, but such a mode produces effect
Rate is low, adds the work load of staff.
The content of the invention
In order to solve the above technical problems, the present invention proposes lipolytic activity cell transplants instrument automatically, production is improved to reach
The purpose of the work load of efficiency and the public personnel of reduction.
To reach above-mentioned purpose, technical scheme is as follows：
A kind of lipolytic activity cell transplants instrument automatically, includes inside instrument body, the instrument body provided with the first filter
Bucket and the second lauter tub, are realized by transmission pipeline between first lauter tub and the second lauter tub and connected, the one of the transmission pipeline
End is placed in the side wall bottom of first lauter tub, and the other end is placed in the side wall top of second lauter tub, first lauter tub
Outside is provided with funnel type drainage part, and the transmission pipeline is passed through on the side wall of the funnel type drainage part, first lauter tub
Top be provided with grease inlet, the second lauter tub bottom is provided with out fat pipeline, and drive shaft, the drive are provided with second lauter tub
Cutter are distributed with moving axis, the drive shaft has motor driving.
The present invention realizes the separation of grease and water by the first lauter tub and the second lauter tub, is realized using cutter to precipitation
Grease is smashed, and using acquisition of the fat pipeline realization to grease competent cell is gone out, is reached raising production efficiency and is reduced public
The purpose of the work load of personnel.
As preferred, the grease inlet goes out provided with Entering fat pipeline.
As preferred, the Entering fat pipeline is provided with nutrition port.Synchronously led into Entering fat pipeline by nutrition port
Enter frozen water to be cooled, make the metabolism reduction of fat, it is ensured that fatty quality.
As preferred, the bottom of the instrument body is provided with delivery port, and the delivery port goes out provided with outlet conduit, described
Outlet conduit is provided with control valve.Using the water filtered out in control valve and outlet conduit realization concentrate at extraction
Reason.
As preferred, the cutter include positive rotor and reverse rotor, the positive rotor and reverse rotor
With the drive shaft turns.It is easy to smash fat using positive rotor and reverse rotor, it is convenient to obtain.
As preferred, it is described go out fat pipeline end be connected with and can measure draw-out device.By the way that draw-out device can be measured
Realize accurate extraction fat.
As preferred, it is described go out fat pipeline end be provided with apparatus for placing, the top of the instrument body is provided with pressurization
Device, the pressue device has controller to be controlled.Accurate extraction to fat is also realized by pressue device and controller.
As preferred, first lauter tub and the second lauter tub are made of filter screen.The first lauter tub is made using filter screen
With the second lauter tub, it is further provided filter efficiency.
As preferred, first lauter tub and the second lauter tub are fixed on inside the instrument body by support.
The invention has the advantages that：
1. the present invention realizes the separation of grease and water by the first lauter tub and the second lauter tub, realized using cutter to precipitation
Grease smashed, realize acquisition to grease competent cell using fat pipeline is gone out, reach that raising production efficiency and reduction are public
The purpose of the work load of common personnel.
2. the present invention is synchronously passed through frozen water into Entering fat pipeline by nutrition port and cooled, make the metabolism drop of fat
It is low, it is ensured that fatty quality.
3. the present invention carries out concentrating extraction processing using the water filtered out in control valve and outlet conduit realization.
4. the present invention is easy to smash fat using positive rotor and reverse rotor, convenient to obtain.
5. the present invention realizes accurate extraction fat by that can measure draw-out device.
6. the present invention also realizes the accurate extraction to fat by pressue device and controller.
7. the first lauter tub and the second lauter tub is made using filter screen in the present invention, it is further provided filter efficiency.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
The accompanying drawing to be used needed for having technology description is briefly described.
Fig. 1 transplants the structural representation of instrument for the lipolytic activity cell disclosed in the embodiment of the present invention 1 automatically；
Fig. 2 transplants the schematic top plan view of instrument for the lipolytic activity cell disclosed in the embodiment of the present invention 2 automatically；
Fig. 3 is the top view that lipolytic activity cell disclosed in the embodiment of the present invention transplants cutter in instrument automatically；
Numeral and the corresponding component title represented by letter in figure：
1. the funnel type drainage part of 2. first 3. second lauter tub of lauter tub, 4. transmission pipeline of instrument body 5.
6. go out the motor of 7. drive shaft of fat pipeline 8. driving 9. Entering fat pipeline 10. nutrition port
11. the reverse rotor of the positive rotor 14. of 12. control valve of outlet conduit 13.
15. the support of 18. controller of draw-out device 16. apparatus for placing, 17. pressue device 19. can be measured.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described.
Instrument is transplanted automatically the invention provides lipolytic activity cell, and its operation principle is by the first lauter tub and the second lauter tub
The separation of grease and water is realized, is realized using cutter and the grease of precipitation is smashed, is realized using fat pipeline is gone out to grease
The acquisition of competent cell, reaches the purpose for the work load for improving production efficiency and reducing public personnel.
With reference to embodiment and embodiment, the present invention is further detailed explanation.
As shown in Figure 1-Figure 3, a kind of lipolytic activity cell transplants instrument automatically, includes instrument body 1, the instrument body
Inside is provided with the first lauter tub 2 and the second lauter tub 3, is realized and connected by transmission pipeline 4 between first lauter tub and the second lauter tub,
One end of the transmission pipeline is placed in the side wall bottom of first lauter tub, and the other end is placed in the side wall top of second lauter tub
End, the outside of first lauter tub is provided with funnel type drainage part 5, and the transmission pipeline passes through the side of the funnel type drainage part
On wall, the top of first lauter tub is provided with grease inlet, and the second lauter tub bottom is provided with out fat pipeline 6, second lauter tub
Cutter are distributed with interior be provided with drive shaft 7, the drive shaft, and the drive shaft has motor to drive 8.
The present invention realizes the separation of grease and water by the first lauter tub and the second lauter tub, is realized using cutter to precipitation
Grease is smashed, and using acquisition of the fat pipeline realization to grease competent cell is gone out, is reached raising production efficiency and is reduced public
The purpose of the work load of personnel.
It is worth noting that, the grease inlet goes out provided with Entering fat pipeline 9.
It is worth noting that, the Entering fat pipeline is provided with nutrition port 10.By nutrition port into Entering fat pipeline it is same
Step is passed through frozen water and cooled, and makes the metabolism reduction of fat, it is ensured that fatty quality.
It is worth noting that, the bottom of the instrument body is provided with delivery port, the delivery port goes out provided with outlet conduit 11,
The outlet conduit is provided with control valve 12.Concentrated using the water filtered out in control valve and outlet conduit realization
Extraction is handled.
It is worth noting that, the cutter include positive rotor 13 and reverse rotor 14, the positive rotor and anti-
To rotor with the drive shaft turns (one group of cutter can be selected according to actual needs, multigroup cutter also may be selected).Profit
It is easy to smash fat with positive rotor and reverse rotor, it is convenient to obtain.
It is worth noting that, it is described go out fat pipeline end be connected with and can measure draw-out device 15.By the way that extraction can be measured
Device realizes accurate extraction fat.
It is worth noting that, it is described go out fat pipeline end be provided with apparatus for placing 16, the top of the instrument body is provided with
Pressue device 17, the pressue device has controller 18 to be controlled.Also realized to fat by pressue device and controller
It is accurate to extract.
It is worth noting that, first lauter tub and the second lauter tub are made of filter screen.The first filter is made using filter screen
Bucket and the second lauter tub, it is further provided filter efficiency.
It is worth noting that, first lauter tub and the second lauter tub are fixed on inside the instrument body by support 19.
The specifically used step of the present invention is as follows：
Embodiment one：Fat is extracted so that draw-out device can be measured；
As shown in figures 1 and 3：In use, the mixture of grease and water is sent into the first lauter tub 2 by Entering fat pipeline 9
It is middle tentatively to be filtered, grease is cooled while being filled with frozen water by nutrition port 10, makes the activity reduction of grease.First
During step filtering, the water for preventing filtering by funnel type drainage part 5 is entered in the second lauter tub 3, when grease is first
While precipitation in lauter tub, entered by transmission pipeline 4 and secondary filter is carried out in second lauter tub, the grease after filtering can be progressively
The bottom of the second lauter tub is deposited in, now according to the actual needs, grease extraction, and root are carried out using draw-out device can be measured
Factually the need for border, the water in instrument body 1 is discharged by outlet conduit and control valve.
Embodiment two：Fat is extracted in the way of pressue device and controller；
As shown in Figures 2 and 3：In use, the mixture of grease and water is sent into the first lauter tub 2 by Entering fat pipeline 9
It is middle tentatively to be filtered, grease is cooled while being filled with frozen water by nutrition port 10, makes the activity reduction of grease.First
During step filtering, the water for preventing filtering by funnel type drainage part 5 is entered in the second lauter tub 3, when grease is first
While precipitation in lauter tub, entered by transmission pipeline 4 and secondary filter is carried out in second lauter tub, the grease after filtering can be progressively
The bottom of the second lauter tub is deposited in, now according to the actual needs, pressue device 17 is adjusted to instrument body 1 by controller 18
Interior application pressure, forces grease from going out in fat pipeline 6 to be flowed into apparatus for placing 16, completes the acquisition of grease；And according to reality
The need for border, the water in instrument body 1 is discharged by outlet conduit and control valve.
By way of more than, lipolytic activity cell provided by the present invention transplants instrument automatically, passes through the first lauter tub and
Two lauter tubs realize the separation of grease and water, realized using cutter and the grease of precipitation is smashed, and are realized using fat pipeline is gone out
Acquisition to grease competent cell, reaches the purpose for the work load for improving production efficiency and reducing public personnel.
Above-described is only the preferred embodiment that lipolytic activity cell disclosed in this invention transplants instrument automatically, should
Point out, for the person of ordinary skill of the art, without departing from the concept of the premise of the invention, if can also make
Dry modification and improvement, these belong to protection scope of the present invention.
Claims (9)
1. a kind of lipolytic activity cell transplants instrument automatically, it is characterised in that includes and is set inside instrument body, the instrument body
There are the first lauter tub and the second lauter tub, realized and connected by transmission pipeline between first lauter tub and the second lauter tub, the transmission
One end of pipeline is placed in the side wall bottom of first lauter tub, and the other end is placed in the side wall top of second lauter tub, described
The outside of one lauter tub is provided with funnel type drainage part, and the transmission pipeline is passed through on the side wall of the funnel type drainage part, described
The top of first lauter tub is provided with grease inlet, and the second lauter tub bottom is provided with out in fat pipeline, second lauter tub provided with driving
Cutter are distributed with axle, the drive shaft, the drive shaft has motor driving.
2. lipolytic activity cell according to claim 1 transplants instrument automatically, it is characterised in that the grease inlet go out to be provided with into
Fat pipeline.
3. lipolytic activity cell according to claim 1 transplants instrument automatically, it is characterised in that the Entering fat pipeline is provided with
Nutrition port.
4. lipolytic activity cell according to claim 1 transplants instrument automatically, it is characterised in that the bottom of the instrument body
Provided with delivery port, the delivery port goes out provided with outlet conduit, and the outlet conduit is provided with control valve.
5. lipolytic activity cell according to claim 1 transplants instrument automatically, it is characterised in that the cutter include just
To rotor and reverse rotor, the positive rotor and reverse rotor are with the drive shaft turns.
6. lipolytic activity cell according to claim 1 transplants instrument automatically, it is characterised in that it is described go out fat pipeline end
Draw-out device can be measured by being connected with.
7. lipolytic activity cell according to claim 1 transplants instrument automatically, it is characterised in that it is described go out fat pipeline end
Provided with apparatus for placing, the top of the instrument body is provided with pressue device, and the pressue device has controller to be controlled.
8. lipolytic activity cell according to claim 1 transplants instrument automatically, it is characterised in that first lauter tub and second
Lauter tub is made of filter screen.
9. lipolytic activity cell according to claim 1 transplants instrument automatically, it is characterised in that first lauter tub and second
Lauter tub is fixed on inside the instrument body by support.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710197776.0A CN106957816B (en) | 2017-03-29 | 2017-03-29 | Automatic transplanting instrument for fat active cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710197776.0A CN106957816B (en) | 2017-03-29 | 2017-03-29 | Automatic transplanting instrument for fat active cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106957816A true CN106957816A (en) | 2017-07-18 |
| CN106957816B CN106957816B (en) | 2023-11-24 |
Family
ID=59471079
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710197776.0A Active CN106957816B (en) | 2017-03-29 | 2017-03-29 | Automatic transplanting instrument for fat active cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106957816B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110523491A (en) * | 2019-09-30 | 2019-12-03 | 中南大学湘雅医院 | a fat cutter |
| WO2021062967A1 (en) * | 2019-09-30 | 2021-04-08 | 中南大学湘雅医院 | Adipose cutter and all-in-one machine for in-vitro fine granular adipose preparation |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130123747A1 (en) * | 2010-05-20 | 2013-05-16 | Carlo Tremolada | Device and method for preparing tissue, particularly adipose tissue |
| CN103598901A (en) * | 2013-10-24 | 2014-02-26 | 苏雪莲 | Fat transplantation device |
| KR20140057830A (en) * | 2012-11-05 | 2014-05-14 | 브이케이테크(주) | Oil decomposing automatic removal device for purification |
| KR20140075540A (en) * | 2012-12-11 | 2014-06-19 | 주식회사 디올메디케어 | Fat container |
| CN205042446U (en) * | 2015-10-15 | 2016-02-24 | 山东万友工业油脂有限公司 | Grease mediation cauldron is cuted and is filtered dual -purpose oil feeding system |
| WO2016085140A1 (en) * | 2014-11-28 | 2016-06-02 | 이준석 | Fat transfer canister |
| KR101694422B1 (en) * | 2016-06-28 | 2017-01-10 | (주) 굿모닝 바이오 | Filtration vessel for adipose stem cells |
-
2017
- 2017-03-29 CN CN201710197776.0A patent/CN106957816B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130123747A1 (en) * | 2010-05-20 | 2013-05-16 | Carlo Tremolada | Device and method for preparing tissue, particularly adipose tissue |
| KR20140057830A (en) * | 2012-11-05 | 2014-05-14 | 브이케이테크(주) | Oil decomposing automatic removal device for purification |
| KR20140075540A (en) * | 2012-12-11 | 2014-06-19 | 주식회사 디올메디케어 | Fat container |
| CN103598901A (en) * | 2013-10-24 | 2014-02-26 | 苏雪莲 | Fat transplantation device |
| WO2016085140A1 (en) * | 2014-11-28 | 2016-06-02 | 이준석 | Fat transfer canister |
| CN205042446U (en) * | 2015-10-15 | 2016-02-24 | 山东万友工业油脂有限公司 | Grease mediation cauldron is cuted and is filtered dual -purpose oil feeding system |
| KR101694422B1 (en) * | 2016-06-28 | 2017-01-10 | (주) 굿모닝 바이오 | Filtration vessel for adipose stem cells |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110523491A (en) * | 2019-09-30 | 2019-12-03 | 中南大学湘雅医院 | a fat cutter |
| WO2021062967A1 (en) * | 2019-09-30 | 2021-04-08 | 中南大学湘雅医院 | Adipose cutter and all-in-one machine for in-vitro fine granular adipose preparation |
| US11305051B2 (en) | 2019-09-30 | 2022-04-19 | Xiangya Hospital Central South University | Fat cutter and integrated machine for preparation of in vitro fine-particle fat |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106957816B (en) | 2023-11-24 |
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