CN106924456A - One kind is for activating mitochondrial composition - Google Patents

Abstract

The invention discloses a composition for activating mitochondria, which is prepared from the following raw materials in parts by weight: 10-20 parts of coumestrol, 5-10 parts of coenzyme Q10, 10-20 parts of ellagic acid, quercetin 1-3 parts of vegetable, 5-10 parts of grape seed extract, 10-15 parts of milk thistle extract, 10-15 parts of olive leaf extract, 10-15 parts of borage, 3-6 parts of kudzu rice fermentation powder , 4‑8 parts of Ganoderma lucidum spore powder, 10‑15 parts of acetylcarnitine, 5‑10 parts of superfine hickory bark powder. The composition obtained in the present invention can improve the activity of mitochondrial enzymes and increase the quantity of mitochondrial DNA, so the composition can activate mitochondria and increase biosynthesis of mitochondria. Therefore, the composition of the present invention can be effectively used for the prevention or treatment of various diseases associated with decreased mitochondrial activity, such as degenerative diseases, Parkinson's syndrome and the like.

Description

A composition for activating mitochondria

technical field

The present invention specifically relates to a composition for activating mitochondria.

Background technique

Mitochondria are organelles found in the vast majority of eukaryotic cells. Mitochondria have their own DNA, DNA (mtDNA) that is separate from the nuclear DNA.

The most important role of mitochondria is to generate adenosine triphosphate (ATP), which is the energy source in cells. ATP is produced from the electron transport chain through the TCA cycle (tricarboxylic acid cycle) using NADH and FADH2 produced in the mitochondrial matrix. Thus, the ATP produced is used to activate various biosynthetic and metabolic processes requiring energy.

Mitochondria also transiently store calcium ions in the matrix, which are important for intracellular signal transduction, and release calcium ions into the cytoplasm when needed. In addition, mitochondria are known to play central regulatory roles in activities such as apoptosis, cell proliferation, and cell metabolism.

Mitochondrial DNA is relatively vulnerable to damage because, unlike nuclear DNA, it lacks self-repair mechanisms and has no DNA-protecting histones. It is well known that the damage of mitochondrial DNA is closely related to the pathogenesis of mitochondrial diseases. The degradation of mitochondrial function will lead to the reduction of ATP synthesis, which is an energy source required for cell activities, and thus cause various diseases.

Contents of the invention

To solve the above problems, the present invention provides a composition for activating mitochondria.

In order to achieve the above object, the technical scheme that the present invention takes is:

A composition for activating mitochondria, prepared from the following raw materials in parts by weight:

10-20 parts of coumestrol, 5-10 parts of coenzyme Q10, 10-20 parts of ellagic acid, 1-3 parts of quercetin, 5-10 parts of grape seed extract, 10-15 parts of milk thistle extract , 10-15 parts of olive leaf extract, 10-15 parts of borage, 3-6 parts of kudzu rice fermentation powder, 4-8 parts of broken Ganoderma lucidum spore powder, 10-15 parts of acetyl carnitine, hickory bark super 5-10 parts of finely pulverized powder

Wherein, the grape seed extract is prepared through the following steps:

S1. After soaking the grape seeds in salt water for 0.5-1 hour, place them in a flash extractor for treatment. After the flash treatment, add 3-6 times of water, and add neutral protease in a proportion of 0.5-1.5% by mass, 30 Enzymolyze at ~40°C for 0.5~2h, filter to obtain enzymolyzate;

S2, the enzymolysis solution is directly passed through the neutral alumina column, and the diameter-to-height ratio of the resin column is 1:6 to 1:11, and washed with 1 to 3 times of the column volume of water and 1 to 2 times of the column volume of 95% organic solution respectively Remove impurities, elute with 0.3-0.7% glacial acetic acid-50% organic solvent (1:1) solution of 6-10 times column volume, and collect the eluate;

S3, concentrating and drying the eluate to obtain grape seed extract.

Wherein, the milk thistle extract is prepared through the following steps:

Treat the dried milk thistle with a flash extractor, heat the obtained extract first with strong fire, then heat with slow fire for 1-1.5h after boiling, filter and concentrate to 1.5g/ml, slowly add 90-100% ethanol until the concentration of ethanol is reached 55-65%, refrigerated for 8-9 hours, centrifuged at 2000-4000r/min for 15-25min, poured off the supernatant, dissolved the precipitate with deionized water to the original volume, and precipitated with ethanol twice, the ethanol concentration was 65- 75%, 75-85%; dissolve the precipitate with deionized water to the original volume, add 1/6-1/5 volume of 10-12% trichloroacetic acid solution, mix well and let stand for 4-6h, 2000-4000r After centrifuging for 15-25min at 15-25min, the supernatant was obtained by removing the precipitate, and the obtained supernatant was suction-filtered and dried to obtain the milk thistle extract.

Wherein, the olive leaf extract is prepared through the following steps:

Wash the olive leaves, freeze-dry them, crush them, thaw them at room temperature and concentrate them to a relative density of 1.10-1.20, spray-dry the concentrated solution, and perform _CO2 supercritical extraction. The fluid extraction pressure is 30-50MPa, and the extraction temperature is 30-40°C. The CO ₂ fluid flow rate is 750-850L/h, and the extraction time is 3-5h to obtain olive leaf extract.

Wherein, the kudzu rice fermented powder is prepared through the following steps:

Take the washed and drained kudzu rice and put it in the steam explosion tank, first pass nitrogen gas until the pressure in the steam explosion tank is 0.7-1.5MPa, and blast for 7-23 minutes; then quickly inject steam to the pressure in the steam explosion tank 1.5-2.1MPa, after steam explosion treatment for 0.8-2.8min, inoculate the microbial starter according to the proportion of 1.5-1.7% of the inoculum, keep the temperature at 25-35°C, ferment for 48-54 hours, and then sterilize at high temperature to obtain kudzu Rice Baking Powder.

Wherein, the microbial starter is obtained by mixing Lactobacillus, Bacillus licheniformis and Corynebacterium glutamicum in a mass ratio of 3:2:1.

Wherein, the broken ganoderma spore powder is prepared through the following steps:

S1. Put the washed and drained Ganoderma lucidum spores in the steam explosion tank, first feed nitrogen until the pressure in the steam explosion tank is 0.6-1.4MPa, and blast for 8-25 minutes; then quickly inject steam to the pressure in the steam explosion tank 1.4-1.8MPa, steam explosion treatment for 0.5-2.5min, concentrated at room temperature to a relative density of 1.10-1.20, spray-dried the concentrate to obtain powder;

S2. Add 3-8 times of water to the powder, add neutral protease at a mass percentage of 2-3%, add flavor protease at a mass percentage of 1-2%, and enzymatically hydrolyze at 30-50°C for 1-2 hours , filtered to obtain the enzymatic solution;

S3, rapidly freezing the obtained fermented liquid and then thawing naturally to age and precipitate the starch in the extract; collecting the supernatant, centrifuging the remaining part, collecting the centrifuged liquid and merging it into the supernatant;

S4. Pass the supernatant through the macroporous resin column at a flow rate of 3-7BV/h. After the dynamic adsorption is saturated, rinse the above-mentioned macroporous resin column with deionized water at a flow rate of 5-8BV/h until the effluent is colorless. Then use an organic solvent with a volume fraction of 50%-70% for elution at a flow rate of 8-12BV/h, collect the eluate, and freeze-dry to obtain the broken-wall ganoderma spore powder.

The present invention has the following beneficial effects:

The resulting composition can increase the activity of mitochondrial enzymes and increase the amount of mitochondrial DNA, so the composition can activate mitochondria and increase mitochondrial biosynthesis. Therefore, the composition of the present invention can be effectively used for the prevention or treatment of various diseases associated with decreased mitochondrial activity, such as degenerative diseases, Parkinson's syndrome and the like.

detailed description

In order to make the objects and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

In the following examples, the grape seed extract used is prepared through the following steps:

S1. After soaking the grape seeds in salt water for 0.5-1 hour, place them in a flash extractor for treatment. After the flash treatment, add 3-6 times of water, and add neutral protease in a proportion of 0.5-1.5% by mass, 30 Enzymolyze at ~40°C for 0.5~2h, filter to obtain enzymolyzate;

S2, the enzymolysis solution is directly passed through the neutral alumina column, and the diameter-to-height ratio of the resin column is 1:6 to 1:11, and washed with 1 to 3 times of the column volume of water and 1 to 2 times of the column volume of 95% organic solution respectively Remove impurities, elute with 0.3-0.7% glacial acetic acid-50% organic solvent (1:1) solution of 6-10 times column volume, and collect the eluate;

S3, concentrating and drying the eluate to obtain grape seed extract.

Wherein, the milk thistle extract is prepared through the following steps:

Treat the dried milk thistle with a flash extractor, heat the obtained extract first with strong fire, then heat with slow fire for 1-1.5h after boiling, filter and concentrate to 1.5g/ml, slowly add 90-100% ethanol until the concentration of ethanol is reached 55-65%, refrigerated for 8-9 hours, centrifuged at 2000-4000r/min for 15-25min, poured off the supernatant, dissolved the precipitate with deionized water to the original volume, and precipitated with ethanol twice, the ethanol concentration was 65- 75%, 75-85%; dissolve the precipitate with deionized water to the original volume, add 1/6-1/5 volume of 10-12% trichloroacetic acid solution, mix well and let stand for 4-6h, 2000-4000r After centrifuging for 15-25min at 15-25min, the supernatant was obtained by removing the precipitate, and the obtained supernatant was suction-filtered and dried to obtain the milk thistle extract.

Wherein, the olive leaf extract is prepared through the following steps:

Wash the olive leaves, freeze-dry them, crush them, thaw them at room temperature and concentrate them to a relative density of 1.10-1.20, spray-dry the concentrated solution, and perform _CO2 supercritical extraction. The fluid extraction pressure is 30-50MPa, and the extraction temperature is 30-40°C. The CO ₂ fluid flow rate is 750-850L/h, and the extraction time is 3-5h to obtain olive leaf extract.

Wherein, the kudzu rice fermented powder is prepared through the following steps:

Take the washed and drained kudzu rice and put it in the steam explosion tank, first pass nitrogen gas until the pressure in the steam explosion tank is 0.7-1.5MPa, and blast for 7-23 minutes; then quickly inject steam to the pressure in the steam explosion tank 1.5-2.1MPa, after steam explosion treatment for 0.8-2.8min, inoculate the microbial starter according to the proportion of 1.5-1.7% of the inoculum, keep the temperature at 25-35°C, ferment for 48-54 hours, and then sterilize at high temperature to obtain kudzu Rice Baking Powder.

Wherein, the microbial starter is obtained by mixing Lactobacillus, Bacillus licheniformis and Corynebacterium glutamicum in a mass ratio of 3:2:1.

Wherein, the broken ganoderma spore powder is prepared through the following steps:

S1. Put the washed and drained Ganoderma lucidum spores in the steam explosion tank, first feed nitrogen until the pressure in the steam explosion tank is 0.6-1.4MPa, and blast for 8-25 minutes; then quickly inject steam to the pressure in the steam explosion tank 1.4-1.8MPa, steam explosion treatment for 0.5-2.5min, concentrated at room temperature to a relative density of 1.10-1.20, spray-dried the concentrate to obtain powder;

S2. Add 3-8 times of water to the powder, add neutral protease at a mass percentage of 2-3%, add flavor protease at a mass percentage of 1-2%, and enzymatically hydrolyze at 30-50°C for 1-2 hours , filtered to obtain the enzymatic solution;

S3, rapidly freezing the obtained fermented liquid and then thawing naturally to age and precipitate the starch in the extract; collecting the supernatant, centrifuging the remaining part, collecting the centrifuged liquid and merging it into the supernatant;

S4. Pass the supernatant through the macroporous resin column at a flow rate of 3-7BV/h. After the dynamic adsorption is saturated, rinse the above-mentioned macroporous resin column with deionized water at a flow rate of 5-8BV/h until the effluent is colorless. Then use an organic solvent with a volume fraction of 50%-70% for elution at a flow rate of 8-12BV/h, collect the eluate, and freeze-dry to obtain the broken-wall ganoderma spore powder.

Example 1

A composition for activating mitochondria, prepared from the following raw materials in parts by weight:

10 parts coumestrol, 5 parts coenzyme Q10, 10 parts ellagic acid, 1 part quercetin, 5 parts grape seed extract, 10 parts milk thistle extract, 10 parts olive leaf extract, 10 parts borage , 3 parts of kudzu rice fermentation powder, 4 parts of broken ganoderma spore powder, 10 parts of acetylcarnitine, 5 parts of hickory bark superfine powder.

Example 2

A composition for activating mitochondria, prepared from the following raw materials in parts by weight:

20 parts of coumestrol, 10 parts of coenzyme Q10, 20 parts of ellagic acid, 3 parts of quercetin, 10 parts of grape seed extract, 15 parts of milk thistle extract, 15 parts of olive leaf extract, 15 parts of borage , 6 parts of kudzu rice fermentation powder, 8 parts of broken ganoderma spore powder, 15 parts of acetylcarnitine, 10 parts of hickory bark superfine powder.

Example 3

A composition for activating mitochondria, prepared from the following raw materials in parts by weight:

15 parts of coumestrol, 7.5 parts of coenzyme Q10, 15 parts of ellagic acid, 2 parts of quercetin, 7.5 parts of grape seed extract, 12.5 parts of milk thistle extract, 12.5 parts of olive leaf extract, 12.5 parts of borage , 4.5 parts of kudzu rice fermentation powder, 6 parts of broken Ganoderma lucidum spore powder, 12.5 parts of acetylcarnitine, 7.5 parts of hickory bark superfine powder.

The above is only a preferred embodiment of the present invention, it should be pointed out that for those of ordinary skill in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, and these improvements and modifications should also be It is regarded as the protection scope of the present invention.

Claims (7)

1. A composition for activating mitochondria, characterized in that it is prepared from the raw materials of the following parts by weight: Coumestrol 10-20 parts, coenzyme Q105-10 parts, ellagic acid 10-20 parts, quercetin 1-3 parts, grape seed extract 5-10 parts, milk thistle extract 10-15 parts, 10-15 parts of olive leaf extract, 10-15 parts of borage, 3-6 parts of kudzu rice fermentation powder, 4-8 parts of broken Ganoderma lucidum spore powder, 10-15 parts of acetyl carnitine, superfine hickory bark 5-10 parts of pulverized powder. 2. A composition for activating mitochondria as claimed in claim 1, wherein the grape seed extract is prepared through the following steps: S1. After soaking the grape seeds in salt water for 0.5-1 hour, place them in a flash extractor for treatment. After the flash treatment, add 3-6 times of water, and add neutral protease in a proportion of 0.5-1.5% by mass, 30 Enzymolyze at ~40°C for 0.5~2h, filter to obtain enzymolyzate; S2, the enzymolysis solution is directly passed through the neutral alumina column, and the diameter-to-height ratio of the resin column is 1:6 to 1:11, and washed with 1 to 3 times of the column volume of water and 1 to 2 times of the column volume of 95% organic solution respectively Remove impurities, elute with 0.3-0.7% glacial acetic acid-50% organic solvent (1:1) solution of 6-10 times column volume, and collect the eluate; S3, concentrating and drying the eluate to obtain grape seed extract. 3. A composition for activating mitochondria as claimed in claim 1, wherein the milk thistle extract is prepared through the following steps: Treat the dried milk thistle with a flash extractor, heat the obtained extract first with strong fire, then heat with slow fire for 1-1.5h after boiling, filter and concentrate to 1.5g/ml, slowly add 90-100% ethanol until the concentration of ethanol is reached 55-65%, refrigerated for 8-9 hours, centrifuged at 2000-4000r/min for 15-25min, poured off the supernatant, dissolved the precipitate with deionized water to the original volume, and precipitated with ethanol twice, the ethanol concentration was 65- 75%, 75-85%; dissolve the precipitate with deionized water to the original volume, add 1/6-1/5 volume of 10-12% trichloroacetic acid solution, mix well and let stand for 4-6h, 2000-4000r After centrifuging for 15-25min at 15-25min, the supernatant was obtained by removing the precipitate, and the obtained supernatant was suction-filtered and dried to obtain the milk thistle extract. 4. A composition for activating mitochondria as claimed in claim 1, wherein said olive leaf extract is prepared through the following steps: Wash the olive leaves, freeze-dry them, crush them, thaw them at room temperature and concentrate them to a relative density of 1.10-1.20, spray-dry the concentrated solution, and perform _CO2 supercritical extraction. The fluid extraction pressure is 30-50MPa, and the extraction temperature is 30-40°C. The CO ₂ fluid flow rate is 750-850L/h, and the extraction time is 3-5h to obtain olive leaf extract. 5. A kind of composition for activating mitochondria as claimed in claim 1, is characterized in that, described Ge Xianmi fermented powder is prepared through the following steps: Take the washed and drained kudzu rice and put it in the steam explosion tank, first pass nitrogen gas until the pressure in the steam explosion tank is 0.7-1.5MPa, and blast for 7-23 minutes; then quickly inject steam to the pressure in the steam explosion tank 1.5-2.1MPa, after steam explosion treatment for 0.8-2.8min, inoculate the microbial starter according to the proportion of 1.5-1.7% of the inoculum, keep the temperature at 25-35°C, ferment for 48-54 hours, and then sterilize at high temperature to obtain kudzu Rice Baking Powder. 6. A kind of composition for activating mitochondria as claimed in claim 5, is characterized in that, described microbial fermentation agent is mixed by lactic acid bacillus, Bacillus licheniformis, Corynebacterium glutamicum in mass ratio 3:2:1 income. 7. A composition for activating mitochondria as claimed in claim 1, characterized in that said broken ganoderma spore powder is prepared through the following steps: S1. Put the washed and drained Ganoderma lucidum spores in the steam explosion tank, first feed nitrogen until the pressure in the steam explosion tank is 0.6-1.4MPa, and blast for 8-25 minutes; then quickly inject steam to the pressure in the steam explosion tank 1.4-1.8MPa, steam explosion treatment for 0.5-2.5min, concentrated at room temperature to a relative density of 1.10-1.20, spray-dried the concentrate to obtain powder; S2. Add 3-8 times of water to the powder, add neutral protease at a mass percentage of 2-3%, add flavor protease at a mass percentage of 1-2%, and enzymatically hydrolyze at 30-50°C for 1-2 hours , filtered to obtain the enzymatic solution; S3, rapidly freezing the obtained fermented liquid and then thawing naturally to age and precipitate the starch in the extract; collecting the supernatant, centrifuging the remaining part, collecting the centrifuged liquid and merging it into the supernatant; S4. Pass the supernatant through the macroporous resin column at a flow rate of 3-7BV/h. After the dynamic adsorption is saturated, rinse the above-mentioned macroporous resin column with deionized water at a flow rate of 5-8BV/h until the effluent is colorless. Then use an organic solvent with a volume fraction of 50%-70% for elution at a flow rate of 8-12BV/h, collect the eluate, and freeze-dry to obtain the broken-wall ganoderma spore powder.