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CN106872408A - A kind of planktonic organism imaging detection device - Google Patents

A kind of planktonic organism imaging detection device Download PDF

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CN106872408A
CN106872408A CN201710280533.3A CN201710280533A CN106872408A CN 106872408 A CN106872408 A CN 106872408A CN 201710280533 A CN201710280533 A CN 201710280533A CN 106872408 A CN106872408 A CN 106872408A
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plankton
laser
color camera
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light
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CN106872408B (en
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王凤鹏
王兴权
曾祥志
谢应茂
李亿保
李勋
范小林
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Gannan Normal University
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    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01MEASURING; TESTING
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    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract

本发明公开了一种浮游生物成像检测装置。该装置包括:第一激光器、第二激光器、彩色相机,第一激光器发出的光被分光棱镜分为参考光和物光,参考光进入所述彩色相机中,物光经过浮游生物成像检测区域后,进入彩色相机中,参考光与物光在彩色相机中的相互干涉;第二激光器发出的光经过浮游生物成像检测区域后,进入彩色相机中;第一激光器所发出的光与第二激光器所发出的光的颜色不同,彩色相机在一次曝光中,能够同时采集到照射到彩色相机的参考光、物光和第二激光器所发出的经过浮游生物成像检测区域的光。本发明提供的浮游生物成像检测装置能够实现对水质较浑浊条件下的浮游生物的高分辨率的原位成像检测。

The invention discloses a plankton imaging detection device. The device includes: a first laser, a second laser, and a color camera. The light emitted by the first laser is divided into reference light and object light by a dichroic prism. The reference light enters the color camera, and the object light passes through the plankton imaging detection area. , into the color camera, the mutual interference between the reference light and the object light in the color camera; the light emitted by the second laser passes through the plankton imaging detection area, and then enters the color camera; the light emitted by the first laser and the light emitted by the second laser The colors of the emitted light are different. In one exposure, the color camera can simultaneously collect the reference light and object light irradiated to the color camera and the light emitted by the second laser that passes through the plankton imaging detection area. The plankton imaging detection device provided by the invention can realize high-resolution in-situ imaging detection of plankton under the condition of relatively turbid water quality.

Description

一种浮游生物成像检测装置A plankton imaging detection device

技术领域technical field

本发明涉及水体中的浮游生物的成像检测领域,特别是涉及一种浮游生物成像检测装置。The invention relates to the field of imaging detection of plankton in a water body, in particular to an imaging detection device for plankton.

背景技术Background technique

当前,对浮游生物的检测方法一般是利用采样网收集浮游生物,带回实验室进行检测,但这样对浮游生物会产生一些不可避免的损伤。也有利用水样采集器将含有浮游生物的水样一起带回实验室进行检测,但采样工作量特别大。目前,一般的水下摄像机由于没有显微成像功能,所以,只能用于大型的水生生物进行成像。对于体形大小在1mm以下的浮游生物,一般需要显微放大才能对其进行成像检测,但显微镜的景深很小,不适合用于对在水体3维空间游动的浮游生物进行成像检测。At present, the detection method for plankton is generally to collect plankton by sampling net and bring it back to the laboratory for detection, but this will cause some inevitable damage to plankton. There are also water sample collectors used to bring back water samples containing plankton to the laboratory for testing, but the sampling workload is particularly heavy. At present, general underwater cameras can only be used for imaging large-scale aquatic organisms because they do not have a microscopic imaging function. For plankton whose body size is less than 1mm, microscopic magnification is generally required to image and detect them, but the depth of field of the microscope is very small, so it is not suitable for imaging and detecting plankton swimming in the three-dimensional space of the water body.

目前现有的技术是分别使用同轴数字全息或离轴数字全息对浮游生物进行成像检测。但由于同轴数字全息只适合于透明背景上的稀疏物体的成像,在水质比较浑浊的时候不能适用。离轴数字全息可以将物体的再现像与共轭像分离,但要求待测物体与图像传感器之间的距离较大,在不加光学放大透镜的情况下,系统的数值孔径较小,导致成像分辨率较低,若加入光学放大透镜,则会导致成像视场较小。可见,现有技术还不能解决对水质较浑浊条件下的浮游生物进行高分辨率的原位成像的问题。At present, the existing technology is to use on-axis digital holography or off-axis digital holography to image and detect plankton. However, coaxial digital holography is only suitable for the imaging of sparse objects on a transparent background, and cannot be applied when the water quality is relatively turbid. Off-axis digital holography can separate the reconstructed image of the object from the conjugate image, but requires a large distance between the object to be measured and the image sensor. Without an optical magnifying lens, the numerical aperture of the system is small, resulting in imaging resolution If the optical magnification lens is added, the imaging field of view will be smaller. It can be seen that the prior art cannot solve the problem of high-resolution in-situ imaging of plankton under the condition of relatively turbid water quality.

发明内容Contents of the invention

本发明的目的是提供一种浮游生物成像检测装置,能够实现对水质较浑浊条件下的浮游生物的高分辨率的原位成像检测。The purpose of the present invention is to provide a plankton imaging detection device, which can realize high-resolution in-situ imaging detection of plankton under the condition of relatively turbid water quality.

为实现上述目的,本发明提供了如下方案:To achieve the above object, the present invention provides the following scheme:

一种浮游生物成像检测装置,所述装置包括:第一激光器、第二激光器、彩色相机,所述第一激光器发出的光被分光棱镜分为参考光和物光,所述参考光进入所述彩色相机中,所述物光经过浮游生物成像检测区域后,进入所述彩色相机中,所述参考光与所述物光在所述彩色相机中的相互干涉;所述第二激光器发出的光经过所述浮游生物成像检测区域后,进入所述彩色相机中;所述第一激光器所发出的光与所述第二激光器所发出的光的颜色不同,所述彩色相机在一次曝光中,能够同时采集到照射到所述彩色相机的所述参考光、所述物光和所述第二激光器所发出的经过所述浮游生物成像检测区域的光。A plankton imaging detection device, the device includes: a first laser, a second laser, a color camera, the light emitted by the first laser is divided into reference light and object light by a beam splitting prism, and the reference light enters the In the color camera, the object light enters the color camera after passing through the plankton imaging detection area, and the reference light and the object light interfere with each other in the color camera; the light emitted by the second laser After passing through the plankton imaging detection area, it enters the color camera; the light emitted by the first laser is different from the light emitted by the second laser, and the color camera can, in one exposure, At the same time, the reference light irradiated to the color camera, the object light and the light emitted by the second laser passing through the plankton imaging detection area are collected.

可选的,所述浮游生物成像检测装置外部包裹有密封壳,所述密封壳上设置有第一光学窗口和第二光学窗口。Optionally, the plankton imaging detection device is wrapped with a sealed shell, and the sealed shell is provided with a first optical window and a second optical window.

可选的,所述浮游生物成像检测装置还包括:第一光纤、第二光纤、第一准直透镜、第二准直透镜、第一分光棱镜、第二分光棱镜、第三分光棱镜、第一反射镜、第二反射镜;所述第一激光器发出的光经过第一光纤输出后,经所述第一准直透镜后变成平行光,再由所述第一分光棱镜分成两束,其中,一束为所述参考光,另一束为所述物光,所述参考光经所述第一反射镜反射,再经所述第二分光棱镜反射到所述彩色相机的图像传感器上,所述物光经过所述第三分光棱镜反射后透过所述第一光学窗口进入所述密封壳外部的所述浮游生物成像检测区,再经所述第二光学窗口进入所述密封壳内,再透过所述第二分光棱镜照射到所述彩色相机的图像传感器上,与所述参考光相互干涉;所述第二激光器发出的光经过所述第二光纤输出后,经所述第二准直透镜后变成平行光,经所述第二反射镜反射后透过所述第三分光棱镜,再透过所述第一光学窗口进入所述密封壳外部的所述浮游生物成像检测区,再经所述第二光学窗口进入所述密封壳内,最后透过所述第二分光棱镜照射到所述彩色相机的图像传感器上,所述彩色相机在一次曝光中,能够同时采集到照射到所述彩色相机的所述参考光、所述物光和所述第二激光器所发出的经过所述浮游生物成像检测区域的光。Optionally, the plankton imaging detection device further includes: a first optical fiber, a second optical fiber, a first collimating lens, a second collimating lens, a first dichroic prism, a second dichroic prism, a third dichroic prism, a A reflecting mirror and a second reflecting mirror; the light emitted by the first laser is output through the first optical fiber, then becomes parallel light after passing through the first collimating lens, and then is divided into two beams by the first dichroic prism, Wherein, one beam is the reference light, and the other beam is the object light, the reference light is reflected by the first mirror, and then reflected by the second dichroic prism onto the image sensor of the color camera , the object light is reflected by the third dichroic prism and enters the plankton imaging detection area outside the sealed shell through the first optical window, and then enters the sealed shell through the second optical window irradiates the image sensor of the color camera through the second dichroic prism, and interferes with the reference light; the light emitted by the second laser is output through the second optical fiber, then passes through the After the second collimating lens becomes parallel light, after being reflected by the second reflector, it passes through the third dichroic prism, and then passes through the first optical window and enters the plankton outside the sealed shell for imaging The detection area enters the sealed shell through the second optical window, and finally shines on the image sensor of the color camera through the second dichroic prism, and the color camera can simultaneously capture The reference light, the object light and the light emitted by the second laser that pass through the plankton imaging detection area are irradiated to the color camera.

可选的,所述浮游生物成像检测装置还包括控制器,所述控制器用于控制所述第一激光器和所述第二激光器的亮度以及所述彩色相机的动作。Optionally, the plankton imaging detection device further includes a controller, the controller is used to control the brightness of the first laser and the second laser and the action of the color camera.

可选的,所述浮游生物成像检测装置还包括电池电源,所述电池电源用于向所述第一激光器、所述第二激光器和所述彩色相机供电。Optionally, the plankton imaging detection device further includes a battery power supply, and the battery power supply is used to supply power to the first laser, the second laser and the color camera.

可选的,所述浮游生物成像检测装置还包括控制监视器,所述控制监视器通过电缆分别与所述控制器、所述彩色相机相连接。Optionally, the plankton imaging detection device further includes a control monitor, and the control monitor is respectively connected to the controller and the color camera through cables.

可选的,所述控制监视器包括监视器和中央处理器,所述监视器用于显示所述彩色相机获取的图像以及所述彩色相机、第一激光器、第二激光器的参数;所述中央处理器用于设置所述第一激光器、第二激光器的参数以及通过所述控制器控制所述彩色相机拍摄全息图片或视频,并根据所述全息图片或视频完成所述浮游生物成像检测区域内浮游生物的成像。Optionally, the control monitor includes a monitor and a central processing unit, the monitor is used to display the image acquired by the color camera and the parameters of the color camera, the first laser and the second laser; the central processing The device is used to set the parameters of the first laser and the second laser, and control the color camera to take holographic pictures or videos through the controller, and complete the plankton imaging and detection of plankton in the area according to the holographic pictures or videos of imaging.

可选的,所述控制监视器还设置有充电接口和图像视频导出接口,所述充电接口用于为电源电池进行充电,所述图像视频导出接口用于导出所述彩色相机拍摄的图片或视频。Optionally, the control monitor is also provided with a charging interface and an image and video export interface, the charging interface is used to charge the power battery, and the image and video export interface is used to export pictures or videos taken by the color camera .

根据本发明提供的具体实施例,本发明公开了以下技术效果:本发明提供的浮游生物成像检测装置采用基于双波长同轴离轴复合数字全息技术实现对水体中的浮游生物进行原位成像检测,利用两种不同波长的激光光源分别形成离轴数字全息图和同轴数字全息图,利用彩色相机单曝光(一次曝光)实现同时记录离轴数字全息图和同轴数字全息图,根据同时记录的离轴数字全息图和同轴数字全息图,完成水质较浑浊条件下的浮游生物的高分辨率成像。According to the specific embodiments provided by the present invention, the present invention discloses the following technical effects: The plankton imaging detection device provided by the present invention uses dual-wavelength coaxial off-axis composite digital holography technology to realize in-situ imaging detection of plankton in the water body , using two laser light sources with different wavelengths to form off-axis digital holograms and on-axis digital holograms respectively, using a single exposure (one exposure) of a color camera to simultaneously record off-axis digital holograms and on-axis digital holograms, according to simultaneous recording The off-axis digital hologram and on-axis digital hologram can complete high-resolution imaging of plankton under turbid water conditions.

附图说明Description of drawings

为了更清楚地说明本发明实施例或现有技术中的技术方案,下面将对实施例中所需要使用的附图作简单地介绍,显而易见地,下面描述中的附图仅仅是本发明的一些实施例,对于本领域普通技术人员来讲,在不付出创造性劳动性的前提下,还可以根据这些附图获得其他的附图。In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the accompanying drawings required in the embodiments. Obviously, the accompanying drawings in the following description are only some of the present invention. Embodiments, for those of ordinary skill in the art, other drawings can also be obtained according to these drawings without paying creative labor.

图1为本发明实施例水下浮游生物成像检测装置结构示意图;Fig. 1 is a schematic structural diagram of an underwater plankton imaging detection device according to an embodiment of the present invention;

图2为本发明实施例控制监视器结构示意图。Fig. 2 is a schematic structural diagram of a control monitor according to an embodiment of the present invention.

具体实施方式detailed description

下面将结合本发明实施例中的附图,对本发明实施例中的技术方案进行清楚、完整地描述,显然,所描述的实施例仅仅是本发明一部分实施例,而不是全部的实施例。基于本发明中的实施例,本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例,都属于本发明保护的范围。The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

本发明的目的是提供一种浮游生物成像检测装置,能够实现对水质较浑浊条件下的浮游生物的高分辨率的原位成像检测。The purpose of the present invention is to provide a plankton imaging detection device, which can realize high-resolution in-situ imaging detection of plankton under the condition of relatively turbid water quality.

为使本发明的上述目的、特征和优点能够更加明显易懂,下面结合附图和具体实施方式对本发明作进一步详细的说明。In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

图1为本发明实施例水下浮游生物成像检测装置结构示意图,如图1所示,浮游生物成像检测装置包括:第一激光器101、第二激光器112、彩色相机107,所述第一激光器101发出的光被分光棱镜分为参考光和物光,所述参考光进入所述彩色相机107中,所述物光经过浮游生物成像检测区域110后,进入所述彩色相机107中,所述参考光与所述物光在所述彩色相机中的相互干涉;所述第二激光器112发出的光经过所述浮游生物成像检测区域110后,进入所述彩色相机107中;所述第一激光器101所发出的光与所述第二激光器112所发出的光的颜色不同(红、绿、蓝三种颜色中的两种),所述彩色相机107在一次曝光中,能够同时采集到照射到所述彩色相机的所述参考光、所述物光和所述第二激光器112所发出的经过所述浮游生物成像检测区域111后的光。所述浮游生物成像检测装置外部包裹有密封壳116,所述密封壳116上设置有第一光学窗口109和第二光学窗口112。Fig. 1 is a schematic structural diagram of an underwater plankton imaging detection device according to an embodiment of the present invention. As shown in Fig. The emitted light is divided into reference light and object light by the dichroic prism, the reference light enters the color camera 107, the object light enters the color camera 107 after passing through the plankton imaging detection area 110, and the reference light enters the color camera 107. The mutual interference between light and the object light in the color camera; the light emitted by the second laser 112 enters the color camera 107 after passing through the plankton imaging detection area 110; the first laser 101 The emitted light is different in color from the light emitted by the second laser 112 (two of the three colors of red, green, and blue), and the color camera 107 can simultaneously collect images of the emitted light in one exposure. The reference light of the color camera, the object light and the light emitted by the second laser 112 after passing through the plankton imaging detection area 111 . The plankton imaging detection device is wrapped with a sealed shell 116 , and the sealed shell 116 is provided with a first optical window 109 and a second optical window 112 .

具体的,第一激光器101发出的光经过第一光纤102输出后,经所述第一准直透镜103后变成平行光,再由所述第一分光棱镜104分成两束,其中,一束为所述参考光,另一束为所述物光,所述参考光经所述第一反射镜105反射,再经所述第二分光棱镜106反射到所述彩色相机107的图像传感器上,所述物光经过所述第三分光棱镜108反射后透过所述第一光学窗口109进入所述密封壳外部的所述浮游生物成像检测区110,再经所述第二光学窗口111进入所述密封壳内,再透过所述第二分光棱镜106照射到所述彩色相机107的图像传感器上,与所述参考光相互干涉形成离轴数字全息图;所述第二激光器112发出的光经过所述第二光纤113输出后,经所述第二准直透镜114后变成平行光,经所述第二反射镜115反射后透过所述第三分光棱镜108,再透过所述第一光学窗口109进入所述密封壳外部的所述浮游生物成像检测区110,再经所述第二光学窗口111进入所述密封壳内,最后透过所述第二分光棱镜106照射到所述彩色相机107的图像传感器上,形成同轴数字全息图;彩色相机107在一次曝光中,能够同时采集到照射到所述彩色相机的所述参考光、所述物光和所述第二激光器112所发出的经过所述浮游生物成像检测区域110后的光。Specifically, after the light emitted by the first laser 101 is output through the first optical fiber 102, it becomes parallel light after passing through the first collimating lens 103, and then is divided into two beams by the first dichroic prism 104, wherein one beam is the reference light, the other beam is the object light, the reference light is reflected by the first reflector 105, and then reflected by the second dichroic prism 106 onto the image sensor of the color camera 107, After being reflected by the third dichroic prism 108, the object light enters the plankton imaging detection area 110 outside the sealed enclosure through the first optical window 109, and then enters the plankton imaging detection area 110 through the second optical window 111. In the above-mentioned sealed shell, and then pass through the second dichroic prism 106 to irradiate the image sensor of the color camera 107, and interfere with the reference light to form an off-axis digital hologram; the light emitted by the second laser 112 After being output by the second optical fiber 113, it becomes parallel light after passing through the second collimating lens 114, is reflected by the second reflector 115, passes through the third dichroic prism 108, and then passes through the The first optical window 109 enters the plankton imaging detection area 110 outside the sealed casing, enters the sealed casing through the second optical window 111, and finally shines on the plankton through the second dichroic prism 106. On the image sensor of the color camera 107, a coaxial digital hologram is formed; in one exposure, the color camera 107 can simultaneously collect the reference light, the object light and the second laser that irradiate the color camera The light emitted by 112 after passing through the plankton imaging detection area 110 .

浮游生物成像检测装置还包括控制器116,所述控制器116用于控制所述第一激光器101和所述第二激光器112的亮度以及所述彩色相机107的动作,所述动作包括:如控制彩色相机107曝光、拍摄全息图、视频等。The plankton imaging detection device also includes a controller 116, the controller 116 is used to control the brightness of the first laser 101 and the second laser 112 and the action of the color camera 107, the action includes: such as controlling The color camera 107 exposes and shoots holograms, videos, and the like.

所述浮游生物成像检测装置还包括电池电源117,所述电池电源117用于向所述第一激光器101、所述第二激光器112和所述彩色107相机供电。The plankton imaging detection device also includes a battery power supply 117, and the battery power supply 117 is used to supply power to the first laser 101, the second laser 112 and the color 107 camera.

所述浮游生物成像检测装置还包括控制监视器119,所述控制监视器119通过电缆120分别与所述控制器117、所述彩色相机107相连接。The plankton imaging detection device also includes a control monitor 119 , and the control monitor 119 is respectively connected to the controller 117 and the color camera 107 through cables 120 .

图2为本发明实施例控制监视器结构示意图,如图2所示,所述控制监视器119包括监视器201和中央处理器,所述监视器201用于显示所述彩色相机107获取的图像以及所述彩色相107、第一激光器101、第二激光器112的参数;所述中央处理器用于设置所述第一激光器101、第二激光器112的参数以及通过所述控制器117控制所述彩色相机107拍摄全息图片或视频,并根据所述全息图片或视频完成所述浮游生物成像检测区域110内浮游生物的成像。Fig. 2 is a schematic structural diagram of a control monitor according to an embodiment of the present invention. As shown in Fig. 2 , the control monitor 119 includes a monitor 201 and a central processing unit, and the monitor 201 is used to display images obtained by the color camera 107 And the parameters of the color phase 107, the first laser 101, and the second laser 112; The camera 107 takes a holographic picture or a video, and completes the imaging of the plankton in the plankton imaging detection area 110 according to the holographic picture or video.

控制监视器119还设置有调节键盘203,可用于对彩色相机107和激光器的参数的设定。图片记录按键202和视频记录按键204用于操作拍摄全息图片或全息图视频。所述控制监视器119还设置有充电接口和图像视频导出接口,所述充电接口用于为电源电池118进行充电,所述图像视频导出接口用于导出所述彩色相机107拍摄的图片或视频。The control monitor 119 is also provided with an adjustment keyboard 203, which can be used to set the parameters of the color camera 107 and the laser. The picture record button 202 and the video record button 204 are used to operate and shoot a hologram or a hologram video. The control monitor 119 is also provided with a charging interface and an image and video export interface, the charging interface is used for charging the power battery 118 , and the image and video export interface is used for exporting pictures or videos taken by the color camera 107 .

使用时,利用承重缆绳将水下浮游生物成像检测装置投放到水中,通过控制监视器操作记录数字全息图或数字全息视频,导入计算机后通过再现程序利用双波长同轴离轴复合数字全息再现算法对全息图或视频进行再现,在程序中再现距离可以从Z1自动变化到Z2(Z1为彩色相机图像传感器到光学窗口2之间的距离,Z2为彩色相机图像传感器到光学窗口1之间的距离),即可实现对成像检测区内的浮游生物进行成像检测。When in use, use the load-bearing cable to put the underwater plankton imaging detection device into the water, record the digital hologram or digital holographic video by controlling the monitor operation, import it into the computer and use the dual-wavelength coaxial off-axis composite digital holographic reproduction algorithm through the reproduction program For hologram or video reproduction, the reproduction distance can be changed automatically from Z1 to Z2 in the program (Z1 is the distance between the color camera image sensor and the optical window 2, Z2 is the distance between the color camera image sensor and the optical window 1 ), the imaging detection of plankton in the imaging detection area can be realized.

本发明基于双波长同轴离轴复合数字全息技术,采用从离轴数字全息图(Ioff)重建得到的图像传感器平面(Recordingplane)内物光波的低精度相位分布φoff作为同轴数字全息图(Iin)相位恢复迭代重建的初始相位分布,在待测物体(Object)平面内加入了从离轴数字全息图(Ioff)重建得到的物光波的低精度相位分布φ0-off作为同轴数字全息图(Iin)相位恢复迭代重建的约束条件。因此,本发明适合于水质比较浑浊或浮游生物(如藻类)浓度较高的情况下对水体中体型大小在10微米到1毫米范围内的浮游生物进行成像检测。而且,本发明具有较高的分辨率和较大的视场,具有检测效率高的特点。其分辨率大小约等彩色相机图像传感器的像素大小,成像视场等于图像传感器的感光面积。每拍摄一张全息图可检测的水体体积为V=S*d,其中,S为图像传感器的感光面积,d为光学窗口1到光学窗口2之间的距离。The present invention is based on the dual-wavelength coaxial off-axis composite digital holography technology, and uses the low-precision phase distribution φoff of the object light wave in the image sensor plane (Recording plane) reconstructed from the off-axis digital hologram (Ioff) as the coaxial digital hologram (Iin ) phase recovery and iterative reconstruction of the initial phase distribution, adding the low-precision phase distribution φ0-off of the object light wave reconstructed from the off-axis digital hologram (Ioff) in the plane of the object to be measured (Object) as the coaxial digital hologram ( Iin) Constraints for iterative reconstruction of phase recovery. Therefore, the present invention is suitable for imaging detection of plankton in the water body with a body size ranging from 10 microns to 1 mm when the water quality is relatively turbid or the concentration of plankton (such as algae) is high. Moreover, the invention has higher resolution and larger field of view, and has the characteristics of high detection efficiency. Its resolution is about the same as the pixel size of a color camera image sensor, and the imaging field of view is equal to the photosensitive area of the image sensor. The volume of the water body that can be detected by taking a hologram is V=S*d, where S is the photosensitive area of the image sensor, and d is the distance between the optical window 1 and the optical window 2 .

本说明书中各个实施例采用递进的方式描述,每个实施例重点说明的都是与其他实施例的不同之处,各个实施例之间相同相似部分互相参见即可。对于实施例公开的系统而言,由于其与实施例公开的方法相对应,所以描述的比较简单,相关之处参见方法部分说明即可。Each embodiment in this specification is described in a progressive manner, each embodiment focuses on the difference from other embodiments, and the same and similar parts of each embodiment can be referred to each other. As for the system disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and for the related information, please refer to the description of the method part.

本文中应用了具体个例对本发明的原理及实施方式进行了阐述,以上实施例的说明只是用于帮助理解本发明的方法及其核心思想;同时,对于本领域的一般技术人员,依据本发明的思想,在具体实施方式及应用范围上均会有改变之处。综上所述,本说明书内容不应理解为对本发明的限制。In this paper, specific examples have been used to illustrate the principle and implementation of the present invention. The description of the above embodiments is only used to help understand the method of the present invention and its core idea; meanwhile, for those of ordinary skill in the art, according to the present invention Thoughts, there will be changes in specific implementation methods and application ranges. In summary, the contents of this specification should not be construed as limiting the present invention.

Claims (8)

1.一种浮游生物成像检测装置,其特征在于,所述装置包括:第一激光器、第二激光器、彩色相机,所述第一激光器发出的光被分光棱镜分为参考光和物光,所述参考光进入所述彩色相机中,所述物光经过浮游生物成像检测区域后,进入所述彩色相机中,所述参考光与所述物光在所述彩色相机中的相互干涉;所述第二激光器发出的光经过所述浮游生物成像检测区域后,进入所述彩色相机中;所述第一激光器所发出的光与所述第二激光器所发出的光的颜色不同,所述彩色相机在一次曝光中,能够同时采集到照射到所述彩色相机的所述参考光、所述物光和所述第二激光器所发出的经过所述浮游生物成像检测区域的光。1. A plankton imaging detection device, characterized in that, said device comprises: a first laser, a second laser, a color camera, and the light emitted by said first laser is divided into reference light and object light by a spectroscopic prism, so The reference light enters the color camera, the object light enters the color camera after passing through the plankton imaging detection area, and the reference light and the object light interfere with each other in the color camera; The light emitted by the second laser enters the color camera after passing through the plankton imaging detection area; the light emitted by the first laser is different from the light emitted by the second laser, and the color camera In one exposure, the reference light irradiated to the color camera, the object light and the light emitted by the second laser passing through the plankton imaging detection area can be collected simultaneously. 2.根据权利要求1所述的浮游生物成像检测装置,其特征在于,所述浮游生物成像检测装置外部包裹有密封壳,所述密封壳上设置有第一光学窗口和第二光学窗口。2 . The plankton imaging detection device according to claim 1 , characterized in that, the plankton imaging detection device is wrapped with a sealed shell, and the sealed shell is provided with a first optical window and a second optical window. 3 . 3.根据权利要求2所述的浮游生物成像检测装置,其特征在于,所述浮游生物成像检测装置还包括:第一光纤、第二光纤、第一准直透镜、第二准直透镜、第一分光棱镜、第二分光棱镜、第三分光棱镜、第一反射镜、第二反射镜;所述第一激光器发出的光经过第一光纤输出后,经所述第一准直透镜后变成平行光,再由所述第一分光棱镜分成两束,其中,一束为所述参考光,另一束为所述物光,所述参考光经所述第一反射镜反射,再经所述第二分光棱镜反射到所述彩色相机的图像传感器上,所述物光经过所述第三分光棱镜反射后透过所述第一光学窗口进入所述密封壳外部的所述浮游生物成像检测区,再经所述第二光学窗口进入所述密封壳内,再透过所述第二分光棱镜照射到所述彩色相机的图像传感器上,与所述参考光相互干涉;所述第二激光器发出的光经过所述第二光纤输出后,经所述第二准直透镜后变成平行光,经所述第二反射镜反射后透过所述第三分光棱镜,再透过所述第一光学窗口进入所述密封壳外部的所述浮游生物成像检测区,再经所述第二光学窗口进入所述密封壳内,最后透过所述第二分光棱镜照射到所述彩色相机的图像传感器上,所述彩色相机在一次曝光中,能够同时采集到照射到所述彩色相机的所述参考光、所述物光和所述第二激光器所发出的经过所述浮游生物成像检测区域的光。3. The plankton imaging detection device according to claim 2, characterized in that, the plankton imaging detection device also comprises: a first optical fiber, a second optical fiber, a first collimating lens, a second collimating lens, a first A beam-splitting prism, a second beam-splitting prism, a third beam-splitting prism, a first reflector, and a second reflector; the light emitted by the first laser passes through the first optical fiber and then passes through the first collimating lens to become The parallel light is divided into two beams by the first dichroic prism, wherein, one beam is the reference beam, and the other beam is the object beam, and the reference beam is reflected by the first reflector, and then passed through the The second dichroic prism is reflected onto the image sensor of the color camera, and the object light passes through the first optical window after being reflected by the third dichroic prism and enters the plankton imaging detection outside the sealed casing. area, and then enter the sealed shell through the second optical window, and then irradiate the image sensor of the color camera through the second dichroic prism, and interfere with the reference light; the second laser After the emitted light is output through the second optical fiber, it becomes parallel light after passing through the second collimating lens, is reflected by the second reflector, passes through the third dichroic prism, and then passes through the first An optical window enters the plankton imaging detection area outside the sealed enclosure, then enters the sealed enclosure through the second optical window, and finally illuminates the image of the color camera through the second dichroic prism On the sensor, the color camera can simultaneously collect the reference light irradiated to the color camera, the object light and the light emitted by the second laser passing through the plankton imaging detection area in one exposure. Light. 4.根据权利要求1所述的浮游生物成像检测装置,其特征在于,所述浮游生物成像检测装置还包括控制器,所述控制器用于控制所述第一激光器和所述第二激光器的亮度以及所述彩色相机的动作。4. The plankton imaging detection device according to claim 1, characterized in that, the plankton imaging detection device further comprises a controller, and the controller is used to control the brightness of the first laser and the second laser and the action of said color camera. 5.根据权利要求1所述的浮游生物成像检测装置,其特征在于,所述浮游生物成像检测装置还包括电池电源,所述电池电源用于向所述第一激光器、所述第二激光器和所述彩色相机供电。5. The plankton imaging detection device according to claim 1, characterized in that, the plankton imaging detection device also includes a battery power supply, and the battery power supply is used to supply the first laser, the second laser and The color camera is powered. 6.根据权利要求1所述的浮游生物成像检测装置,其特征在于,所述浮游生物成像检测装置还包括控制监视器,所述控制监视器通过电缆分别与所述控制器、所述彩色相机相连接。6. The plankton imaging detection device according to claim 1, characterized in that, the plankton imaging detection device also includes a control monitor, and the control monitor is respectively connected to the controller and the color camera through cables. connected. 7.根据权利要求6所述的浮游生物成像检测装置,其特征在于,所述控制监视器包括监视器和中央处理器,所述监视器用于显示所述彩色相机获取的图像以及所述彩色相机、第一激光器、第二激光器的参数;所述中央处理器用于设置所述第一激光器、第二激光器的参数以及通过所述控制器控制所述彩色相机拍摄全息图片或视频,并根据所述全息图片或视频完成所述浮游生物成像检测区域内浮游生物的成像。7. The plankton imaging detection device according to claim 6, wherein the control monitor comprises a monitor and a central processing unit, and the monitor is used to display the image obtained by the color camera and the color camera , the parameters of the first laser and the second laser; the central processing unit is used to set the parameters of the first laser and the second laser and control the color camera to take holographic pictures or videos through the controller, and according to the The holographic picture or video completes the imaging of the plankton in the plankton imaging detection area. 8.根据权利要求6所述的浮游生物成像检测装置,其特征在于,所述控制监视器还设置有充电接口和图像视频导出接口,所述充电接口用于为电源电池进行充电,所述图像视频导出接口用于导出所述彩色相机拍摄的图片或视频。8. The plankton imaging detection device according to claim 6, wherein the control monitor is also provided with a charging interface and an image and video export interface, the charging interface is used to charge the power battery, and the image The video export interface is used to export pictures or videos taken by the color camera.
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019127090A1 (en) * 2017-12-27 2019-07-04 中国科学院深圳先进技术研究院 Underwater plankton optical imaging device and method
CN110118774A (en) * 2019-06-25 2019-08-13 赣南师范大学 A kind of water floating biological monitor and method
CN110261309A (en) * 2019-07-02 2019-09-20 东北大学秦皇岛分校 A magnetic optical coherence tomography system and its magnetic field modulation method
CN110487223A (en) * 2019-08-20 2019-11-22 大连海事大学 A kind of micro- plastics detection device and method based on spatial correlation Yu phase difference value product

Citations (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0676902A2 (en) * 1994-04-04 1995-10-11 Projectavision, Inc. A high efficiency light valve projection system
CN1971253A (en) * 2006-10-19 2007-05-30 上海大学 Digital holographic micro-measuring device
US20080137933A1 (en) * 2005-06-29 2008-06-12 University Of South Florida Variable Tomographic Scanning with Wavelength Scanning Digital Interface Holography
KR100838586B1 (en) * 2007-10-17 2008-06-19 (주)펨트론 3D measuring device and 3D measuring method using digital holography
CN201251551Y (en) * 2008-09-25 2009-06-03 中国海洋大学 On-line visual monitoring device of underwater plankton
US20090147334A1 (en) * 2007-12-10 2009-06-11 Industrial Technology Research Institute Multi-color off-axis digital holographic system and the imaging method thereof
US20100110260A1 (en) * 2007-03-22 2010-05-06 Apntech Co., Ltd. Device and method for recording and reconstructing digital hologram without virtual image
US20100309465A1 (en) * 2009-06-05 2010-12-09 The Penn State Research Foundation Coherent anti-stokes raman holography
CN102183490A (en) * 2011-01-31 2011-09-14 上海大学 Optical fiber holographic interference measuring device
CN102402172A (en) * 2011-11-30 2012-04-04 昆明理工大学 Three-dimensional real time super-resolution digital holography recording system
EP2527928A1 (en) * 2010-01-22 2012-11-28 Hyogo Prefectural Government Generation method for complex amplitude in-line hologram and image recording device using said method
CN103279024A (en) * 2013-05-27 2013-09-04 王凤鹏 Digital holographic recording device for detecting water surface microorganism
CN104834201A (en) * 2015-05-13 2015-08-12 北京工业大学 Dual-wavelength polarization multiplexing digital holographic imaging system and method
CN105277136A (en) * 2015-09-29 2016-01-27 南京理工大学 Transmission-type microscopic imaging device and method based on double-wavelength digital holographic technology
CN105717774A (en) * 2016-04-18 2016-06-29 中国工程物理研究院激光聚变研究中心 Real-time recording apparatus and method for colorful digital holographic image
CN205538708U (en) * 2015-12-30 2016-08-31 中国工程物理研究院激光聚变研究中心 Optical component high depth of field surface defect detection device for transmission dual-wavelength holography
CN106325032A (en) * 2016-09-28 2017-01-11 中国石油大学(华东) A digital holographic recording device with real-time precision adjustable off-axis angle
JP2017076038A (en) * 2015-10-14 2017-04-20 学校法人 関西大学 Digital holography apparatus and digital holography method
CN206710306U (en) * 2017-04-26 2017-12-05 赣南师范大学 A kind of planktonic organism imaging detection device

Patent Citations (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0676902A2 (en) * 1994-04-04 1995-10-11 Projectavision, Inc. A high efficiency light valve projection system
US20080137933A1 (en) * 2005-06-29 2008-06-12 University Of South Florida Variable Tomographic Scanning with Wavelength Scanning Digital Interface Holography
CN1971253A (en) * 2006-10-19 2007-05-30 上海大学 Digital holographic micro-measuring device
US20100110260A1 (en) * 2007-03-22 2010-05-06 Apntech Co., Ltd. Device and method for recording and reconstructing digital hologram without virtual image
KR100838586B1 (en) * 2007-10-17 2008-06-19 (주)펨트론 3D measuring device and 3D measuring method using digital holography
US20090147334A1 (en) * 2007-12-10 2009-06-11 Industrial Technology Research Institute Multi-color off-axis digital holographic system and the imaging method thereof
CN201251551Y (en) * 2008-09-25 2009-06-03 中国海洋大学 On-line visual monitoring device of underwater plankton
US20100309465A1 (en) * 2009-06-05 2010-12-09 The Penn State Research Foundation Coherent anti-stokes raman holography
EP2527928A1 (en) * 2010-01-22 2012-11-28 Hyogo Prefectural Government Generation method for complex amplitude in-line hologram and image recording device using said method
CN102183490A (en) * 2011-01-31 2011-09-14 上海大学 Optical fiber holographic interference measuring device
CN102402172A (en) * 2011-11-30 2012-04-04 昆明理工大学 Three-dimensional real time super-resolution digital holography recording system
CN103279024A (en) * 2013-05-27 2013-09-04 王凤鹏 Digital holographic recording device for detecting water surface microorganism
CN104834201A (en) * 2015-05-13 2015-08-12 北京工业大学 Dual-wavelength polarization multiplexing digital holographic imaging system and method
CN105277136A (en) * 2015-09-29 2016-01-27 南京理工大学 Transmission-type microscopic imaging device and method based on double-wavelength digital holographic technology
JP2017076038A (en) * 2015-10-14 2017-04-20 学校法人 関西大学 Digital holography apparatus and digital holography method
CN205538708U (en) * 2015-12-30 2016-08-31 中国工程物理研究院激光聚变研究中心 Optical component high depth of field surface defect detection device for transmission dual-wavelength holography
CN105717774A (en) * 2016-04-18 2016-06-29 中国工程物理研究院激光聚变研究中心 Real-time recording apparatus and method for colorful digital holographic image
CN106325032A (en) * 2016-09-28 2017-01-11 中国石油大学(华东) A digital holographic recording device with real-time precision adjustable off-axis angle
CN206710306U (en) * 2017-04-26 2017-12-05 赣南师范大学 A kind of planktonic organism imaging detection device

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CAO L 等: "Hybrid digital holographic imaging system for three-dimensional dense particle field measurement", 《APPLIED OPTICS》 *
李明: "激光全息技术的发展及应用趋势研究", 《激光杂志》 *
王凤鹏 等: "数字全息视频成像技术及其在肝吸虫尾蚴观测中的应用", 《光子学报》 *
谢建军等: "离轴无透镜傅里叶变换数字全息三维物场重建", 《激光杂志》 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019127090A1 (en) * 2017-12-27 2019-07-04 中国科学院深圳先进技术研究院 Underwater plankton optical imaging device and method
CN110118774A (en) * 2019-06-25 2019-08-13 赣南师范大学 A kind of water floating biological monitor and method
CN110118774B (en) * 2019-06-25 2024-02-20 赣南师范大学 Water plankton detection device and method
CN110261309A (en) * 2019-07-02 2019-09-20 东北大学秦皇岛分校 A magnetic optical coherence tomography system and its magnetic field modulation method
CN110261309B (en) * 2019-07-02 2021-09-28 东北大学秦皇岛分校 Magnetomotive optical coherence tomography system and magnetic field modulation method thereof
CN110487223A (en) * 2019-08-20 2019-11-22 大连海事大学 A kind of micro- plastics detection device and method based on spatial correlation Yu phase difference value product
CN110487223B (en) * 2019-08-20 2021-04-23 大连海事大学 A microplastic detection device and method based on the product of spatial correlation and phase difference value

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