CN106879832A - Improve the feed addictive and its preparation method of Funiu White Goat speed of weight increment and immunity - Google Patents
Improve the feed addictive and its preparation method of Funiu White Goat speed of weight increment and immunity Download PDFInfo
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- CN106879832A CN106879832A CN201611261612.1A CN201611261612A CN106879832A CN 106879832 A CN106879832 A CN 106879832A CN 201611261612 A CN201611261612 A CN 201611261612A CN 106879832 A CN106879832 A CN 106879832A
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- 241000283707 Capra Species 0.000 title claims abstract description 33
- 230000036039 immunity Effects 0.000 title claims abstract description 26
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 238000000855 fermentation Methods 0.000 claims abstract description 36
- 230000004151 fermentation Effects 0.000 claims abstract description 36
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 15
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229960000281 trometamol Drugs 0.000 claims abstract description 15
- 229920001218 Pullulan Polymers 0.000 claims abstract description 14
- 239000004373 Pullulan Substances 0.000 claims abstract description 14
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims abstract description 14
- 235000019423 pullulan Nutrition 0.000 claims abstract description 14
- 229960001471 sodium selenite Drugs 0.000 claims abstract description 14
- 235000015921 sodium selenite Nutrition 0.000 claims abstract description 14
- 239000011781 sodium selenite Substances 0.000 claims abstract description 14
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 12
- 239000002609 medium Substances 0.000 claims description 17
- 239000002054 inoculum Substances 0.000 claims description 12
- 230000004913 activation Effects 0.000 claims description 10
- 238000011534 incubation Methods 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 5
- 239000001963 growth medium Substances 0.000 claims description 5
- 238000011218 seed culture Methods 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 239000000654 additive Substances 0.000 claims description 4
- 230000000996 additive effect Effects 0.000 claims description 4
- FFEARJCKVFRZRR-UHFFFAOYSA-N L-Methionine Natural products CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 claims description 3
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims description 3
- 229930195722 L-methionine Natural products 0.000 claims description 3
- 229960004452 methionine Drugs 0.000 claims description 3
- FFEARJCKVFRZRR-SCSAIBSYSA-N D-methionine Chemical compound CSCC[C@@H](N)C(O)=O FFEARJCKVFRZRR-SCSAIBSYSA-N 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims description 2
- 201000010099 disease Diseases 0.000 abstract description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 7
- 239000000203 mixture Substances 0.000 abstract description 5
- 239000011669 selenium Substances 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 229910052711 selenium Inorganic materials 0.000 description 3
- 229940091258 selenium supplement Drugs 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 239000009636 Huang Qi Substances 0.000 description 2
- 241000830535 Ligustrum lucidum Species 0.000 description 2
- 241000218378 Magnolia Species 0.000 description 2
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 2
- 244000197580 Poria cocos Species 0.000 description 2
- 235000008599 Poria cocos Nutrition 0.000 description 2
- 229910021536 Zeolite Inorganic materials 0.000 description 2
- 239000003674 animal food additive Substances 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
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- 238000004519 manufacturing process Methods 0.000 description 2
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- 239000010457 zeolite Substances 0.000 description 2
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 240000004307 Citrus medica Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 244000202052 Poncirus trifoliata Species 0.000 description 1
- 235000000404 Poncirus trifoliata Nutrition 0.000 description 1
- 241000282849 Ruminantia Species 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 235000019730 animal feed additive Nutrition 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000020411 cell activation Effects 0.000 description 1
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- 239000003795 chemical substances by application Substances 0.000 description 1
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- 238000003912 environmental pollution Methods 0.000 description 1
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- 230000036737 immune function Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
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- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
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- 230000008569 process Effects 0.000 description 1
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- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of feed, more particularly, to the feed formula for improving goat resistance against diseases.The invention aims to provide a kind of feed formula for improving goat resistance against diseases.Solving the technical scheme of its problem is：Improve the feed addictive of Funiu White Goat speed of weight increment and immunity, including following percentage by weight composition:20 60 parts of Se-enriched yeast, 15 parts of tromethamine, 0.2 2 parts of pullulan, 20 40 parts of sodium selenite.The preparation method of the feed addictive of Funiu White Goat speed of weight increment and immunity is improved, is comprised the following steps：1）Saccharomycete culture；2）Shake flask fermentation culture；3）Fermentation tank culture.
Description
Technical field
The present invention relates to a kind of animal feed additive, more particularly, to raising Funiu White Goat speed of weight increment and immunity
Feed addictive and its preparation method.
Background technology
Funiu White Goat is Henan Province's Local Excellent kind, is mainly distributed on the Funiu Shan Mountain in Western Henan Province and the west and south
Area.Funiu White Goat has that meat production is good, dressing percentage is high, reproductive performance good, resistance to thick feeding, premunition are strong, suitable mountain region is herded
The advantages of.
Some diseases are frequently encountered in the feeding process of Funiu White Goat, treatment disease not in time can cause serious
Loss.But the diagnosis of the disease in actual operation needs to overcome more suffering, could correctly accomplish, therefore in reality
The prophylactic discovery of operation, improve Funiu White Goat resistance against diseases seem of crucial importance.Traditional method is using anti-
Raw element class medicine.But because the generation of drug resistance, antibiotic are accumulated in vivo, the harm to chicken health is had resulted in, and
Excessive antibiotic is excreted to the environmental pollution caused in environment, and then influence is will also result on human health, thus both at home and abroad
Begin one's study biological control or bionomic control, green feed additive is developed, in the hope of solving the above problems.
The Chinese patent of application number 201210486329.4 discloses a kind of raising Funiu White Goat speed of weight increment and is immunized
The feed addictive and its preparation method of power, improve the feed addictive and its preparation method of Funiu White Goat speed of weight increment and immunity, institute
The feed addictive stated is made up of the Radix Astragali, dried orange peel, malt, the bark of official magnolia, the dried immature fruit of citron orange, rhizoma atractylodis, the fruit of glossy privet, Poria cocos and zeolite, and each component is accounted for
The mass percent of feed addictive total amount is：Radix Astragali 28%-52%, dried orange peel 20%-30%, malt 8%-12%, bark of official magnolia 9%-11%, trifoliate orange
Real 4%-6%, rhizoma atractylodis 4%-6%, fruit of glossy privet 1%-3%, Poria cocos 1%-2%, zeolite 1%-2%.Chinese herb feed additive of the invention can show
Production performance, the immunity of organisms for improving Funiu White Goat are write, its feedstuff-meat ratio is reduced；And the immune of Funiu White Goat can be promoted
Competent cell activation, so as to improve the immunologic function of body.
There is the effect on driving birds is not good of Chinese medicine in the technology, the raw material for using are relatively more, the more expensive problem of cost of material.
The content of the invention
The invention aims to provide, using effect is good, improve the feed of Funiu White Goat speed of weight increment and immunity adds
Plus agent and its preparation method.
Solving the technical scheme of its problem is：
Improve the feed addictive of Funiu White Goat speed of weight increment and immunity, including following percentage by weight composition：
Se-enriched yeast 20-60 parts, tromethamine 1-5 parts, pullulan 0.2-2 parts, sodium selenite 20-40 parts.
Preferably, 40 parts of the optimum proportioning Se-enriched yeast of described additive, 1 part of tromethamine, 0.2 part of pullulan,
30 parts of sodium selenite.
Preferably, described additive is added by 0.3mg/kg.
The preparation method of the feed addictive of Funiu White Goat speed of weight increment and immunity is improved, is comprised the following steps：
1）Saccharomycete culture：Accessed after inclined-plane saccharomycete activation 4h in seed culture medium, cultivate 10-20h, cultivation temperature 20-30
DEG C, shaking speed 150-200r/min；
2）Shake flask fermentation culture：To fermentation medium（40mL/500mL）In press 10%（v/v）Inoculum concentration access activation yeast
Shake flask fermentation culture, 23-27 DEG C of temperature, shaking speed 100-200r/min, incubation time 30h are carried out after bacterium.Through filtration sterilization
（0.25 μm of filter membrane diameter）Tromethamine, pullulan, sodium selenite solution add fermentation medium in, while need addition
L-Methionine；
3）Fermentation tank culture：Full-automatic 5L stirred-tank fermenters（BIOTECH-5BGZ, it is public that emerging biological plant engineering finite is protected in Shanghai
Department）Middle dress fermentation medium 3L, inoculum concentration 10%（v/v）, speed of agitator 350r/min, 23-27 DEG C of temperature, the L/ of throughput 3.0
Min, incubation time 25-30h.Determine pH.
Preferably, step 1）：Culture 20h, 30 DEG C of cultivation temperature, shaking speed 200r/min；
Preferably, step 2）：Shake flask fermentation culture, 27 DEG C of temperature, shaking speed 200r/min；
Preferably, step 2）：L-Methionine is added in 0h, and addition concentration is 10 m mol/L；
Preferably, step 3）：Dress fermentation medium 3L, inoculum concentration 10%（v/v）, speed of agitator 350r/min, 27 DEG C of temperature, lead to
The L/min of tolerance 3.0；
Preferably, step 3）：PH is detected in place using plum Teller electrode, by the mol/LH of auto-feeding 32SO4Or 3 mol/
LNaOH solution is adjusted maintaining pH to change within ± 0.02.
Beneficial effects of the present invention：The feed addictive for improving Funiu White Goat speed of weight increment and immunity of the invention and
Its preparation method, by adding Se-enriched yeast, in Se-enriched yeast and tromethamine, pullulan, sodium selenite intermixture group whole blood
The vigor of GSH-Px and the content of serum selenium are significantly increased, and particularly Se-enriched yeast has biology higher to ruminant
Utilization rate, therefore selenium content is few in excreta, is conducive to environmental protection.Tromethamine and pullulan also have enhancing anti-after combining
The effect of sick ability.Selenium has humidification to animal cell immunity function, can improve the conversion of periphery blood lymphocyte simultaneously
Ability, improves T cell quantity in lymphocyte, so as to strengthen the premunition of body, can improve the antioxidation of body, reduces
The destruction of free radical and MDA to cell membrane integrity, so as to improve the immunity and resistance against diseases of body.
Specific embodiment
The present invention is described further in conjunction with the embodiments.
Embodiment one
A kind of feed addictive for improving Funiu White Goat speed of weight increment and immunity, including following percentage by weight composition：
Se-enriched yeast 20-30 parts, tromethamine 1-5 parts, pullulan 0.2-2 parts, sodium selenite 20-30 parts.
The preparation method of the feed addictive of Funiu White Goat speed of weight increment and immunity is improved, is comprised the following steps：
1）Saccharomycete culture：Accessed after inclined-plane saccharomycete activation 4h in seed culture medium, cultivate 10-15h, cultivation temperature 20-25
DEG C, shaking speed 150-180r/min；
2）Shake flask fermentation culture：To fermentation medium（40mL/500mL）In press 10%（v/v）Inoculum concentration access activation yeast
Shake flask fermentation culture, 23-27 DEG C of temperature, shaking speed 100-200r/min, incubation time 30h are carried out after bacterium.Through filtration sterilization
（0.25 μm of filter membrane diameter）Tromethamine, pullulan, sodium selenite solution add fermentation medium in, L-Methionine is in 0h
Addition, addition concentration is 10 m mol/L；
3）Fermentation tank culture：Full-automatic 5L stirred-tank fermenters（BIOTECH-5BGZ, it is public that emerging biological plant engineering finite is protected in Shanghai
Department）Middle dress fermentation medium 3L, inoculum concentration 10%（v/v）, speed of agitator 350r/min, 27 DEG C of temperature, the L/min of throughput 3.0,
Incubation time 30h.PH is detected in place using plum Teller electrode, molten by the mol/LH2SO4 of auto-feeding 3 or 3 mol/LNaOH
Liquid is adjusted maintaining pH to change within ± 0.02.
Embodiment two
A kind of feed addictive for improving Funiu White Goat speed of weight increment and immunity, including following percentage by weight composition：
30 parts of Se-enriched yeast, 1 part of tromethamine, 0.2 part of pullulan, 30 parts of sodium selenite.
The preparation method of the feed addictive of Funiu White Goat speed of weight increment and immunity is improved, is comprised the following steps：
1）Saccharomycete culture：Accessed after inclined-plane saccharomycete activation 4h in seed culture medium, cultivate 20h, 30 DEG C of cultivation temperature, shaking table
Rotating speed 200r/min；
2）Shake flask fermentation culture：To fermentation medium（40mL/500mL）In press 10%（v/v）Inoculum concentration access activation yeast
Shake flask fermentation culture, 27 DEG C of temperature, shaking speed 200r/min, incubation time 30h are carried out after bacterium.Through filtration sterilization（Filter membrane is straight
0.25 μm of footpath）Tromethamine, pullulan, sodium selenite solution add fermentation medium in, while need add L- egg ammonia
Acid；
3）Fermentation tank culture：Full-automatic 5L stirred-tank fermenters（BIOTECH-5BGZ, it is public that emerging biological plant engineering finite is protected in Shanghai
Department）Middle dress fermentation medium 3L, inoculum concentration 10%（v/v）, speed of agitator 350r/min, 27 DEG C of temperature, the L/min of throughput 3.0,
Incubation time 30h.PH is detected in place using plum Teller electrode, by the mol/LH of auto-feeding 32SO4Or 3 mol/LNaOH solution
It is adjusted maintaining pH to change within ± 0.02.
Embodiment three
A kind of feed addictive for improving Funiu White Goat speed of weight increment and immunity, including following percentage by weight composition:
40 parts of Se-enriched yeast, 5 parts of tromethamine, 2 parts of pullulan, 40 parts of sodium selenite.
The preparation method of the feed addictive of Funiu White Goat speed of weight increment and immunity is improved, is comprised the following steps：
1）Saccharomycete culture：Accessed after inclined-plane saccharomycete activation 4h in seed culture medium, cultivate 25h, 28 DEG C of cultivation temperature, shaking table
Rotating speed 150r/min；
2）Shake flask fermentation culture：To fermentation medium（40mL/500mL）In press 10%（v/v）Inoculum concentration access activation yeast
Shake flask fermentation culture, 25 DEG C of temperature, shaking speed 150r/min, incubation time 23h are carried out after bacterium.Through filtration sterilization（Filter membrane is straight
0.25 μm of footpath）Tromethamine, during pullulan part, sodium selenite solution add fermentation medium, while needing to add L- eggs
Propylhomoserin；
3）Fermentation tank culture：Full-automatic 5L stirred-tank fermenters（BIOTECH-5BGZ, it is public that emerging biological plant engineering finite is protected in Shanghai
Department）Middle dress fermentation medium 3L, inoculum concentration 10%（v/v）, speed of agitator 300r/min, 23 DEG C of temperature, the L/min of throughput 3.0,
Incubation time 30h.PH is detected in place using plum Teller electrode, by the mol/LH of auto-feeding 32SO4Or 3 mol/LNaOH solution
It is adjusted maintaining pH to change within ± 0.02.
In this description, the present invention is described with reference to its specific embodiment.But it is clear that can still make
Various modifications and alterations are without departing from the spirit and scope of the present invention.Therefore, specification is considered as illustrative rather than limit
Property processed.
Claims (8)
1. the feed addictive of Funiu White Goat speed of weight increment and immunity is improved, it is characterised in that：Its raw material is by weight such as
Under：Se-enriched yeast 20-60 parts, tromethamine 1-5 parts, pullulan 0.2-2 parts, sodium selenite 20-40 parts.
2. the preparation method of the feed addictive of Funiu White Goat speed of weight increment and immunity is improved, is comprised the following steps：
1）Saccharomycete culture：Accessed after inclined-plane saccharomycete activation 4h in seed culture medium, cultivate 10-20h, cultivation temperature 20-30
DEG C, shaking speed 150-200r/min；
2）Shake flask fermentation culture：Shake flask fermentation is carried out after the saccharomycete that activation is accessed to the inoculum concentration in fermentation medium by 10%
Culture, 23-27 DEG C of temperature, shaking speed 100-200r/min, incubation time 30h.Through the tromethamine of filtration sterilization, grow sturdily it is mould many
Sugar, sodium selenite solution are added in fermentation medium, while needing to add L-Methionine；
3）Fermentation tank culture：Fermentation medium 3L, inoculum concentration 10%, speed of agitator 350r/ are filled in full-automatic 5L stirred-tank fermenters
Min, 23-27 DEG C of temperature, throughput 3.0 L/min, incubation time 25-30h.
3. the feed addictive for improving Funiu White Goat speed of weight increment and immunity according to claim 1, its feature exists
In：40 parts of the optimum proportioning Se-enriched yeast of described additive, 1 part of tromethamine, 0.2 part of pullulan, 30 parts of sodium selenite.
4. the feed addictive for improving Funiu White Goat speed of weight increment and immunity according to claim 1, its feature exists
In：Described additive is added by 0.3 mg/kg.
5. the preparation method of the feed addictive for improving Funiu White Goat speed of weight increment and immunity according to claim 2, its
It is characterised by：Step 1）：Culture 20h, 30 DEG C of cultivation temperature, shaking speed 200r/min.
6. the preparation method of the feed addictive for improving Funiu White Goat speed of weight increment and immunity according to claim 2, its
It is characterised by：Step 2）：Shake flask fermentation culture, 27 DEG C of temperature, shaking speed 200r/min.
7. the preparation method of the feed addictive for improving Funiu White Goat speed of weight increment and immunity according to claim 2, its
It is characterised by preferred, step 2）：L-Methionine is added in 0h, and addition concentration is 10 m mol/L.
8. the preparation method of the feed addictive for improving Funiu White Goat speed of weight increment and immunity according to claim 2, its
It is characterised by：Step 3）：Dress fermentation medium 3L, inoculum concentration 10%, speed of agitator 350r/min, 27 DEG C of temperature, throughput 3.0
L/min。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611261612.1A CN106879832A (en) | 2016-12-30 | 2016-12-30 | Improve the feed addictive and its preparation method of Funiu White Goat speed of weight increment and immunity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611261612.1A CN106879832A (en) | 2016-12-30 | 2016-12-30 | Improve the feed addictive and its preparation method of Funiu White Goat speed of weight increment and immunity |
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| Publication Number | Publication Date |
|---|---|
| CN106879832A true CN106879832A (en) | 2017-06-23 |
Family
ID=59176467
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611261612.1A Pending CN106879832A (en) | 2016-12-30 | 2016-12-30 | Improve the feed addictive and its preparation method of Funiu White Goat speed of weight increment and immunity |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1522711A (en) * | 2003-02-17 | 2004-08-25 | 表飞鸣制药株式会社 | Enterobacteria containing tablet |
| CN1709133A (en) * | 2005-07-12 | 2005-12-21 | 南京农业大学 | Selenium-rich probiotics foder additive production method and its product |
| CN101535315A (en) * | 2006-10-13 | 2009-09-16 | 辉瑞有限公司 | Heterocyclic compounds useful as anabolic agents for livestock animals |
| CN102090544A (en) * | 2011-01-13 | 2011-06-15 | 司文会 | Selenium-rich goose feed and method for improving organic selenium in goose bodies |
-
2016
- 2016-12-30 CN CN201611261612.1A patent/CN106879832A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1522711A (en) * | 2003-02-17 | 2004-08-25 | 表飞鸣制药株式会社 | Enterobacteria containing tablet |
| CN1709133A (en) * | 2005-07-12 | 2005-12-21 | 南京农业大学 | Selenium-rich probiotics foder additive production method and its product |
| CN101535315A (en) * | 2006-10-13 | 2009-09-16 | 辉瑞有限公司 | Heterocyclic compounds useful as anabolic agents for livestock animals |
| CN102090544A (en) * | 2011-01-13 | 2011-06-15 | 司文会 | Selenium-rich goose feed and method for improving organic selenium in goose bodies |
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Application publication date: 20170623 |