CN106867932A - A kind of mushroom bran poultry manure deodorizing microorganism preparation method - Google Patents

Abstract

A kind of mushroom bran poultry manure deodorizing microorganism preparation method, is related to a kind of deodorizing microorganism preparation method, a kind of mushroom bran poultry manure deodorizing microorganism preparation method, it is characterised in that methods described includes procedure below：First, stacked after being thoroughly mixed in the ratio formula of rice bran 14.5%, oil cake 14%, dregs of beans 13%, carbohydrate 8% and dusty yeast 0.5%, stirring is admixed after fermentation；Secondly, lactic acid bacteria, bacillus, actinomyces and saccharomycete press 1：1：1：1 formula mixing is fabricated to deodorizing microorganism；Finally, mixed deodorizing microorganism is pressed 1:1 ratio, is sprayed on the holder made with mushroom bran carrier.The present invention makees the poultry manure deodorizing microorganism of carrier with mushroom bran, is inhibitory action of the deodorizing microorganism fixed of carrier in the pernicious gas produced during banking up to bio-feritlizers such as poultry manures with mushroom bran packaging technique.

Description

Preparation method of fungus chaff and poultry manure deodorizing bacteria agent

technical field

The invention relates to a preparation method of a deodorizing bacterial agent, in particular to a preparation method of a deodorizing bacterial agent of fungus chaff and poultry manure.

Background technique

Biological deodorization is a method developed in the 1950s to deal with odor. This method has become a research hotspot in many countries due to its high treatment efficiency, no secondary pollution, simple treatment equipment, low cost and easy operation. The method of microbial degradation of odor has incomparable advantages over physical and chemical methods, including soil deodorization method, perlite cotton deodorization method, compost deodorization method, activated sludge deodorization method, sawdust deodorization method, peat deodorization method and EM method.

At present, the sawdust deodorization method is based on the characteristics of sawdust, such as good ventilation, strong adsorption, moderate water retention, and suitable for microbial growth and reproduction. Suitable for low and high content of odor, wide application area, medium deodorization ability, good ventilation, low operating cost, but it needs to change sawdust frequently.

Although sawdust is a waste in the process of wood processing and production, its source is still felled trees, which is contrary to the sustainable development concept of our country. In addition, sawdust has less nutrients and cannot satisfy the normal growth and reproduction of some types of microorganisms.

Contents of the invention

The object of the present invention is to provide a kind of preparation method of poultry feces deodorizing bacterial agent with bacterium chaff, the poultry feces deodorizing bacterial agent that uses bacterial chaff as carrier in the present invention, utilizes bacterial chaff coating technology as carrier fixed deodorizing bacterial agent Inhibition of harmful gases produced by bio-fertilizers such as poultry manure during stacking.

The purpose of the present invention is achieved through the following technical solutions:

A preparation method of fungal chaff poultry manure deodorizing bacterial agent, said method comprising the following processes:

First, according to the formula of 14.5% rice bran, 14% oil cake, 13% soybean meal, 8% sugar and 0.5% yeast powder, it is fully stirred and mixed, stacked, and mixed and stirred after fermentation; secondly, lactic acid bacteria, bacillus, actinomycetes and The yeast is mixed according to the formula of 1:1:1:1 to make deodorant agent; finally, the mixed deodorant agent is sprayed on the support made of fungus chaff carrier according to the ratio of 1:1.

The preparation method of the fungus chaff poultry manure deodorizing bacteria agent is fully stirred according to the ratio of rice bran 14.5%, oil cake 14%, soybean meal 13%, sugar 8%, water 50% and yeast powder 0.5%. Stacking after mixing, blending and stirring after fermentation; the above-mentioned sugars and yeast powder are prepared according to conventional methods.

The preparation method of the poultry feces deodorizing bacterial agent with fungus chaff, the method includes the preparation of the strain: preliminary preparation → propagation of the bacteria → collection of the bacteria;

Preliminary preparation: strains, including lactic acid bacteria, bacillus, actinomycetes, and yeast;

Expansion of bacteria: use the plate streak method to amplify the four strains in the plate to obtain enough experimental materials;

Collect bacteria: save the four amplified bacteria separately; 5ml of lactic acid bacteria, 5ml of Bacillus cereus, 5ml of actinomycetes, and 5ml of yeast; preparation of deodorant bacteria: lactic acid bacteria, bacillus, actinomycetes Mix with yeast according to the formula of 1:1:1:1 to make a deodorant agent; spray the mixed deodorant agent according to the ratio of 1:1 on the support made of fungus chaff carrier, that is The finished fungus bran poultry manure deodorizing bacteria agent is obtained.

The preparation method of a deodorizing bacterial agent for poultry manure with fungus chaff is as follows: preparation of bacterial strains: preliminary preparation → proliferation of bacteria → collection of bacteria;

Preliminary preparation: strains, including lactic acid bacteria, bacillus, actinomycetes, and yeast;

Expansion of bacteria: use the plate streak method to amplify the four strains in the plate to obtain enough experimental materials;

Collect bacteria: save the four amplified bacteria separately;

Lactic acid bacteria 10ml, Bacillus cereus 10ml, actinomycetes 10ml, yeast 10ml; lactic acid bacteria, bacillus, actinomycetes and yeast are mixed according to the formula of 1:1:1:1 to make deodorant bacteria agent; deodorant bacteria The immobilization of the agent was as described above.

The preparation method of the bacteria chaff poultry manure deodorizing bacteria agent, the preparation of the bacteria in the method is as follows: preliminary preparation → propagation of the bacteria → collection of the bacteria;

Preliminary preparation: strains, including lactic acid bacteria, bacillus, actinomycetes, and yeast;

Expansion of bacteria: use the plate streak method to amplify the four strains in the plate to obtain enough experimental materials;

Collect bacteria: save the four amplified bacteria separately;

Lactic acid bacteria 15ml, Bacillus cereus 15ml, actinomycetes 15ml, yeast 15ml; lactic acid bacteria, bacillus, actinomycetes and yeast are mixed according to the formula of 1:1:1:1 to make a deodorant agent; deodorant bacteria The immobilization of the agent was as described above.

Advantage and effect of the present invention are:

Compared with similar bacterial agents, the invention can inhibit spoilage bacteria from producing odorous gases such as ammonia, hydrogen sulfide and methyl indole during the composting process, inhibit the growth of pathogenic bacteria, and reduce root system diseases. At the same time, the treated fertilizer has biological activity and good permeability to the soil, which is conducive to the decomposition of organic matter in the soil, and promotes the absorption of inorganic matter by crop roots, which is beneficial to crop growth.

Another benefit of the present invention is to reuse the waste mushroom chaff after the mushrooms are harvested.

detailed description

The present invention will be described in detail below in conjunction with examples.

First, according to the formula of 14.5% rice bran, 14% oil cake, 13% soybean meal, 8% sugar and 0.5% yeast powder, it is fully stirred and mixed, stacked, and mixed and stirred after fermentation; secondly, lactic acid bacteria, bacillus, actinomycetes and The yeast is mixed according to the formula of 1:1:1:1 to make deodorant agent; finally, the mixed deodorant agent is sprayed on the support made of fungus chaff carrier according to the ratio of 1:1. The invention inhibits spoilage bacteria from producing odorous gases such as ammonia, hydrogen sulfide and methyl indole during the composting process, inhibits the growth of pathogenic bacteria, and reduces root system diseases.

Embodiment 1, the preparation of fermented ripening powder

1. Formulation:

Rice Bran 14.5%, Oil Cake 14%, Soybean Meal 13%

Sugar 8%, Water 50%, Yeast Powder 0.5%

2. Preparation method:

According to the formula of 14.5% rice bran, 14% oil cake, 13% soybean meal, 8% sugar, 50% water and 0.5% yeast powder, the formula is fully stirred and mixed, stacked, and blended and stirred after fermentation.

The above-mentioned sugars and yeast powder are prepared by conventional methods.

Fermentation bag selects polyethylene plastic bag for use and its specification is 15cm*20cm.

Embodiment 2, a kind of bacterium bran poultry excrement deodorizing bacterial agent 1

(1) Preparation of fermented ripening powder as described in Example 1

(2) Preparation of deodorant bacteria agent:

1. Preparation of strains:

Preliminary preparation → proliferation of bacteria → collection of bacteria

Preliminary preparation: strains (lactic acid bacteria, bacillus, actinomycetes, and yeasts) were purchased from the Institute of Microbiology, Chinese Academy of Sciences, and stored at low temperature in the Key Laboratory of Shenyang University.

Expansion of bacteria: the four strains were amplified on the plate by streaking method to obtain enough experimental materials.

Collection of bacterial cells: The four types of amplified bacterial cells were stored separately in the Key Laboratory of Shenyang University.

2. Formulation:

Lactic acid bacteria 5ml, Bacillus cereus 5ml, Actinomycetes 5ml, Yeast 5ml

3. Preparation of deodorant bacteria agent:

Lactic acid bacteria, bacillus, actinomycetes and yeast are mixed according to the formula of 1:1:1:1 to make deodorant bacteria.

(3) Fixation of deodorant bacteria:

Spray the mixed deodorizing bacteria agent on the support made of fungus chaff carrier according to the ratio of 1:1 to obtain the finished fungus chaff and poultry manure deodorizing bacteria agent.

During the spraying process of the bacterial liquid, the deodorant bacterial agent should be evenly sprayed on the bacterial chaff carrier made of fermented ripening powder.

Effect test:

Table 1 The effect of deodorant bacteria on the removal of methyl indole in chicken manure

Table 2 Effect of deodorizing bacteria agent on _NH3 in chicken manure

Embodiment 3, a kind of bacterium bran poultry excrement deodorizing bacterial agent 2

(1) Preparation of fermented ripening powder as described in Example 1

(2) Preparation of deodorant bacteria agent:

1. Preparation of strains:

Preliminary preparation → proliferation of bacteria → collection of bacteria

Preliminary preparation: strains (lactic acid bacteria, bacillus, actinomycetes, and yeasts) were purchased from the Institute of Microbiology, Chinese Academy of Sciences, and stored at low temperature in the Key Laboratory of Shenyang University.

Expansion of bacteria: the four strains were amplified on the plate by streaking method to obtain enough experimental materials.

Collection of bacterial cells: The four types of amplified bacterial cells were stored separately in the Key Laboratory of Shenyang University.

2. Formulation:

Lactic acid bacteria 10ml, Bacillus cereus 10ml

Actinomycetes 10ml, Yeast 10ml

3. Preparation of deodorant bacteria agent:

Lactic acid bacteria, bacillus, actinomycetes and yeast are mixed according to the formula of 1:1:1:1 to make deodorant bacteria.

(3) The fixation of the deodorant bacteria agent is as described in Example 2

Effect test:

Table 1 The effect of deodorant bacteria on the removal of methyl indole in chicken manure

Table 2 The effect of deodorant bacteria on NH3 in chicken manure

Embodiment 4, a kind of bacterium bran poultry excrement deodorizing bacterial agent 3

(1) Preparation of fermented ripening powder as described in Example 1

(2) Preparation of deodorant bacteria agent:

1. Preparation of strains:

Preliminary preparation → proliferation of bacteria → collection of bacteria

Preliminary preparation: strains (lactic acid bacteria, bacillus, actinomycetes, and yeasts) were purchased from the Institute of Microbiology, Chinese Academy of Sciences, and stored at low temperature in the Key Laboratory of Shenyang University.

Expansion of bacteria: the four strains were amplified on the plate by streaking method to obtain enough experimental materials.

Collection of bacterial cells: The four types of amplified bacterial cells were stored separately in the Key Laboratory of Shenyang University.

2. Formulation:

Lactic acid bacteria 15ml, Bacillus cereus 15ml

Actinomycetes 15ml, Yeast 15ml

3. Preparation of deodorant bacteria agent:

Lactic acid bacteria, bacillus, actinomycetes and yeast are mixed according to the formula of 1:1:1:1 to make deodorant bacteria.

(3) The fixation of the deodorant bacteria agent is as described in Example 2

Effect test:

Table 1 The effect of deodorant bacteria on the removal of methyl indole in chicken manure

Table 2 The effect of deodorant bacteria on NH3 in chicken manure

Claims (5)

1. a preparation method of bacterium chaff poultry manure deodorizing bacterial agent, is characterized in that, described method comprises following process: First, according to the formula of 14.5% rice bran, 14% oil cake, 13% soybean meal, 8% sugar and 0.5% yeast powder, it is fully stirred and mixed, stacked, and mixed and stirred after fermentation; secondly, lactic acid bacteria, bacillus, actinomycetes and The yeast is mixed according to the formula of 1:1:1:1 to make deodorant agent; finally, the mixed deodorant agent is sprayed on the support made of fungus chaff carrier according to the ratio of 1:1. 2. A method for preparing a fungus bran poultry manure deodorizing agent according to claim 1, characterized in that, the method comprises 14.5% of rice bran, 14% of oil cake, 13% of soybean meal, 8% of sugar, and 50% of water. The proportion formula of 0.5% with yeast powder is fully stirred and mixed, stacked, and blended and stirred after fermentation; the above-mentioned sugar and yeast powder are prepared according to conventional methods. 3. A method for preparing a fungus chaff and poultry manure deodorizing bacterial agent according to claim 1, characterized in that, the method comprises the preparation of bacteria: preliminary preparation → proliferation of bacteria → collection of bacteria; Preliminary preparation: strains, including lactic acid bacteria, bacillus, actinomycetes, and yeast; Expansion of bacteria: use the plate streak method to amplify the four strains in the plate to obtain enough experimental materials; Collect bacteria: save the four amplified bacteria separately; 5ml of lactic acid bacteria, 5ml of Bacillus cereus, 5ml of actinomycetes, and 5ml of yeast; preparation of deodorant bacteria: lactic acid bacteria, bacillus, actinomycetes Mix it with yeast at a ratio of 1:1:1:1 to make a deodorant agent; spray the mixed deodorant agent at a ratio of 1:1 on the support made of fungus chaff carrier, that is The finished fungus bran poultry manure deodorizing bacteria agent is obtained. 4. a kind of bacterium chaff poultry excrement deodorizing bacterial agent preparation method according to claim 1 is characterized in that, the preparation of described method deodorizing bacterial agent is as follows: Preparation of strains: preliminary preparation → proliferation of bacteria → collection of bacteria; Preliminary preparation: strains, including lactic acid bacteria, bacillus, actinomycetes, and yeast; Expansion of bacteria: use the plate streak method to amplify the four strains in the plate to obtain enough experimental materials; Collect bacteria: save the four amplified bacteria separately; Lactic acid bacteria 10ml, Bacillus cereus 10ml, actinomycetes 10ml, yeast 10ml; lactic acid bacteria, bacillus, actinomycetes and yeast are mixed according to the formula of 1:1:1:1 to make deodorant bacteria agent; deodorant bacteria The immobilization of the agent was as described above. 5. A method for preparing a fungus chaff and poultry manure deodorizing bacterial agent according to claim 1, characterized in that, the preparation of the strains in the method is as follows: preliminary preparation → proliferation of the thalline → collection of the thalline; Preliminary preparation: strains, including lactic acid bacteria, bacillus, actinomycetes, and yeast; Expansion of bacteria: use the plate streak method to amplify the four strains in the plate to obtain enough experimental materials; Collect bacteria: save the four amplified bacteria separately; Lactic acid bacteria 15ml, Bacillus cereus 15ml, actinomycetes 15ml, yeast 15ml; lactic acid bacteria, bacillus, actinomycetes and yeast are mixed according to the formula of 1:1:1:1 to make a deodorant agent; deodorant bacteria The immobilization of the agent was as described above.