CN106831003A - A kind of preparation method of plant source amino acid polypeptide Water soluble fertilizer - Google Patents
A kind of preparation method of plant source amino acid polypeptide Water soluble fertilizer Download PDFInfo
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- CN106831003A CN106831003A CN201710122758.6A CN201710122758A CN106831003A CN 106831003 A CN106831003 A CN 106831003A CN 201710122758 A CN201710122758 A CN 201710122758A CN 106831003 A CN106831003 A CN 106831003A
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- 150000001413 amino acids Chemical class 0.000 title claims abstract description 43
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 36
- 239000003337 fertilizer Substances 0.000 title claims abstract description 31
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 24
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 24
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 239000002002 slurry Substances 0.000 claims abstract description 63
- 238000009413 insulation Methods 0.000 claims abstract description 24
- 239000002253 acid Substances 0.000 claims abstract description 20
- 239000000843 powder Substances 0.000 claims abstract description 17
- 108091005804 Peptidases Proteins 0.000 claims abstract description 13
- 239000004365 Protease Substances 0.000 claims abstract description 13
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 13
- 235000019419 proteases Nutrition 0.000 claims abstract description 13
- 239000003513 alkali Substances 0.000 claims abstract description 12
- 108091005508 Acid proteases Proteins 0.000 claims abstract description 11
- 239000002893 slag Substances 0.000 claims abstract description 11
- 108010011619 6-Phytase Proteins 0.000 claims abstract description 10
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims abstract description 10
- 229940085127 phytase Drugs 0.000 claims abstract description 10
- 238000009835 boiling Methods 0.000 claims abstract description 6
- 238000005119 centrifugation Methods 0.000 claims abstract description 6
- 238000001914 filtration Methods 0.000 claims abstract description 6
- 241000196324 Embryophyta Species 0.000 claims description 47
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 45
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 18
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 18
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 12
- 244000105624 Arachis hypogaea Species 0.000 claims description 12
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 12
- 235000018262 Arachis monticola Nutrition 0.000 claims description 12
- 235000012054 meals Nutrition 0.000 claims description 12
- 235000020232 peanut Nutrition 0.000 claims description 12
- 240000002791 Brassica napus Species 0.000 claims description 11
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims description 11
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 229940088598 enzyme Drugs 0.000 claims description 8
- 150000003839 salts Chemical class 0.000 claims description 8
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- 238000000227 grinding Methods 0.000 claims description 5
- 238000005360 mashing Methods 0.000 claims description 5
- 238000007873 sieving Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 230000007935 neutral effect Effects 0.000 claims 1
- 229940024606 amino acid Drugs 0.000 description 37
- 235000001014 amino acid Nutrition 0.000 description 37
- 239000000126 substance Substances 0.000 description 6
- -1 Valine Chemical compound 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 2
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 2
- 241000894007 species Species 0.000 description 2
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- 101800000263 Acidic protein Proteins 0.000 description 1
- 241000432767 Asparagus setaceus Species 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 1
- FFEARJCKVFRZRR-UHFFFAOYSA-N L-Methionine Natural products CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 description 1
- 229930064664 L-arginine Natural products 0.000 description 1
- 235000014852 L-arginine Nutrition 0.000 description 1
- LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 229930195722 L-methionine Natural products 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 101150039403 ams gene Proteins 0.000 description 1
- QLULGSLAHXLKSR-UHFFFAOYSA-N azane;phosphane Chemical compound N.P QLULGSLAHXLKSR-UHFFFAOYSA-N 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 229960003067 cystine Drugs 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 229960002885 histidine Drugs 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000002440 industrial waste Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229960004452 methionine Drugs 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 229940068041 phytic acid Drugs 0.000 description 1
- 235000002949 phytic acid Nutrition 0.000 description 1
- 239000000467 phytic acid Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 229960002898 threonine Drugs 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Fertilizers (AREA)
Abstract
The present invention relates to a kind of preparation method of plant source amino acid polypeptide Water soluble fertilizer, it is characterised in that：Comprise the following steps：A, in mass ratio 1:4 weigh plant granulated slag and water, are well mixed to obtain Plant Powder slurry；B, to adding acid pectase in Plant Powder slurry, 45 DEG C of insulations adjust pH to 4.5 after 3 hours；Phytase is added, 50 DEG C are incubated 1 hour, then boiling water bath 20 minutes, and it is standby that end temperature is down to 50 DEG C of insulations；Add AMS, 50 DEG C of insulations add acid protease after 1 hour, and 50 DEG C are incubated 3 hours, adjust pH to 7.0；Neutral proteinase is added, 50 DEG C are incubated 3 hours, adjust pH to 10.0；Alkali protease is added, 50 DEG C are incubated 3 hours, adjust pH to 4.2；C, centrifugation, filtering, obtain plant source amino acid polypeptide Water soluble fertilizer.Preparation process of the present invention is simply efficient, and amino acid classes are more in gained Water soluble fertilizer.
Description
Technical field
The present invention relates to new-type fertilizer production technical field, more particularly to a kind of system of plant source amino acid polypeptide Water soluble fertilizer
Preparation Method.
Background technology
Existing Most amino-acids fertilizer product, is all directly to buy amino acid powder and mineral nutrient element, such as urea,
Phosphorus ammonium, potassium dihydrogen phosphate, zinc sulfate, magnesium sulfate, calcium nitrate etc., are simply mixed at high temperature, as amino acids fertilizer, this kind of
Amino acid fertilizer, does not also chelate fully with trace element, and many plant essential amino acid species do not have yet, and fertilization effect is not
It is good.Origin of amino acid is mainly industrial waste, the hydrolysis of hair chemical method in existing amino acids fertilizer, and it exists many
Problem：1st, accessory substance is more, there is carcinogenic risk；2nd, salinity is high, and excessive chloride or sulfate radical is present in system, to making
Thing causes harm；3rd, hydrolyze completely, be unfavorable for that crop root is absorbed, Partial Species amino acid is decomposed in preparation process
Fall.
The content of the invention
The technical problem to be solved in the present invention is to provide that a kind of chemical by-product is few, and salinity is low, rich in several amino acids
The preparation method of plant source amino acid polypeptide Water soluble fertilizer.
In order to solve the above technical problems, the present invention uses following technical scheme：A kind of plant source amino acid polypeptide Water soluble fertilizer
Preparation method, it is characterised in that：Comprise the following steps：
A, in mass ratio 1:4 weigh plant granulated slag and water, load reactor, are uniformly mixed, grinding mashing, 60 mesh mistakes
With salt acid for adjusting pH to 3.0 after sieve, 45 DEG C of insulations obtain Plant Powder slurry in 2 hours；The plant granulated slag is dregs of beans, peanut meal, dish
One kind or their mixture in the seed dregs of rice；
B, to acid pectase is added in step A gained Plant Powder slurries, the addition of the acid pectase is plant
Granulated slag slurry quality 0.1%, 45 DEG C insulation 3 hours after with potassium hydroxide adjust reactor in slurries pH to 4.5；Add phytic acid
Enzyme, the addition of the phytase is the 0.7% of Plant Powder slurry quality, and 50 DEG C are incubated 1 hour, then boiling water bath 20 minutes,
It is standby that end temperature is down to 50 DEG C of insulations；AMS is added, the addition of the AMS is Plant Powder slurry quality
0.3%, 50 DEG C of insulations add acid protease after 1 hour, and the addition of the acid protease is Plant Powder slurry quality
1%, 50 DEG C be incubated 3 hours, then with potassium hydroxide adjust reactor slurries pH to 7.0；Add neutral proteinase, it is described in
Property protease addition for Plant Powder slurry quality 2%, 50 DEG C be incubated 3 hours, add potassium hydroxide regulation reactor
Slurries pH to 10.0；Alkali protease is added, the addition of the alkali protease is the 1% of Plant Powder slurry quality, 50 DEG C
Insulation 3 hours, adds watery hydrochloric acid regulation reactor slurries pH to 4.2；
C, by the centrifugation of step B gained reactor slurries, filtering, obtain plant source amino acid polypeptide Water soluble fertilizer.
The present invention selects dregs of beans as plant source, then using acid pectase, phytase, AMS, acidic protein
Enzyme, neutral proteinase, alkali protease carry out enzyme fermentation reaction, and the water solubility fertilizer rich in amino acid and polypeptide is finally obtained
Material, the fertilizer has the advantages that bioactivity is high, impurity is few.The cooperation that the inventive method passes through different enzymes, enzyme is carried out by dregs of beans
Solution, reduces protein, amino acid, the degraded of polypeptide in dregs of beans, is conducive to absorption of the crops to these nutritional ingredients.The hair
Bright method also has gently efficiently, while can fully degrade dregs of beans, completely remains the organic principle in dregs of beans, Bu Huizao
The degraded of amino acid into dregs of beans, amino acid classes are complete, and gained plant source amino acid polypeptide Water soluble fertilizer can be provided comprehensively for plant
Amino acid nutrient, while reducing traditional chemical routes prepares the harmful components such as the carcinogenic substance, the salt that are produced during amino acid.Through inspection
Survey, up to 18 kinds of amino acid classes in plant source amino acid polypeptide Water soluble fertilizer prepared by the inventive method are specifically included：L- asparagus ferns
Propylhomoserin, L-threonine, serine, glutamic acid, glycine, ALANINE, CYSTINE, Valine, L-Methionine, L- are different bright
Propylhomoserin, L-Leu, TYR, phenylalanine, lysine, L-Histidine, L-Trp, L-arginine, L-PROLINE.
In sum, the present invention uses the plant granulated slags such as various enzyme composite degradation dregs of beans peanut meals, rapeseed dregs, with preparation
Process is simply efficient, while abundant degrading plant granulated slag, can completely remain the organic principle in plant granulated slag, Bu Huizao
The degraded of amino acid into plant granulated slag, amino acid classes are more in gained chemical fertilizer, and salinity is low, and chemical by-product gives birth to the advantages of lacking.
Specific embodiment
Embodiment 1：
A kind of preparation method of plant source amino acid polypeptide Water soluble fertilizer, comprises the following steps：
A, in mass ratio 1:4 weigh dregs of beans and water, load reactor, are uniformly mixed, grinding mashing, after the sieving of 60 mesh
With salt acid for adjusting pH to 3.0,45 DEG C of insulations obtain dregs of beans slurry for 2 hours；
B, starched to step A gained dregs of beans in add acid pectase, the addition of the acid pectase starches matter for dregs of beans
Amount 0.1%, 45 DEG C insulation 3 hours after with potassium hydroxide adjust reactor in slurries pH to 4.5；Add phytase, the plant
The addition of sour enzyme is the 0.7% of dregs of beans slurry quality, and 50 DEG C are incubated 1 hour, then boiling water bath 20 minutes, and end temperature is down to 50
DEG C insulation is standby；AMS is added, the addition of the AMS is the 0.3% of dregs of beans slurry quality, and 50 DEG C are incubated 1 hour
After add acid protease, the addition of the acid protease is the 1% of dregs of beans slurry quality, and 50 DEG C are incubated 3 hours, then
Reactor slurries pH to 7.0 is adjusted with potassium hydroxide；Neutral proteinase is added, the addition of the neutral proteinase is dregs of beans slurry
The 2% of quality, 50 DEG C are incubated 3 hours, add potassium hydroxide regulation reactor slurries pH to 10.0；Add alkali protease,
The addition of the alkali protease is the 1% of dregs of beans slurry quality, and 50 DEG C are incubated 3 hours, adds watery hydrochloric acid regulation reactor slurry
Liquid pH to 4.2；
C, by the centrifugation of step B gained reactor slurries, filtering, obtain plant source amino acid polypeptide Water soluble fertilizer.The gained of embodiment 1
Amino acid classes and content such as table 1 in plant source amino acid polypeptide Water soluble fertilizer.
Table 1
Embodiment 2：
A kind of preparation method of plant source amino acid polypeptide Water soluble fertilizer, comprises the following steps：
A, in mass ratio 1:4 weigh peanut meal and water, load reactor, are uniformly mixed, grinding mashing, the sieving of 60 mesh
Afterwards with salt acid for adjusting pH to 3.0,45 DEG C of insulations obtain peanut meal slurry for 2 hours；
B, starched to step A gained peanut meals in add acid pectase, the addition of the acid pectase is peanut meal
Slurry quality 0.1%, 45 DEG C insulation 3 hours after with potassium hydroxide adjust reactor in slurries pH to 4.5；Add phytase, institute
It is the 0.7% of peanut meal slurry quality to state the addition of phytase, and 50 DEG C are incubated 1 hour, then boiling water bath 20 minutes, end temperature
It is down to 50 DEG C of insulations standby；AMS is added, the addition of the AMS is the 0.3% of peanut meal slurry quality, 50 DEG C
Insulation adds acid protease after 1 hour, the addition of the acid protease is the 1% of peanut meal slurry quality, 50 DEG C of guarantors
Temperature 3 hours, then adjusts reactor slurries pH to 7.0 with potassium hydroxide；Add neutral proteinase, the neutral proteinase plus
It is the 2% of peanut meal slurry quality to enter amount, and 50 DEG C are incubated 3 hours, adds potassium hydroxide regulation reactor slurries pH to 10.0；Plus
Enter alkali protease, the addition of the alkali protease is the 1% of peanut meal slurry quality, and 50 DEG C are incubated 3 hours, add dilute salt
Acid regulation reactor slurries pH to 4.2；
C, by the centrifugation of step B gained reactor slurries, filtering, obtain plant source amino acid polypeptide Water soluble fertilizer.The plant source ammonia
Base acid polypeptide Water soluble fertilizer rich in XXXX, the amino acid such as YYY, WWW.The gained plant source amino acid polypeptide Water soluble fertilizer of embodiment 2
Middle amino acid classes and content such as table 2.
Table 2
Embodiment 3：
A kind of preparation method of plant source amino acid polypeptide Water soluble fertilizer, comprises the following steps：
A, in mass ratio 1:4 weigh rapeseed dregs and water, load reactor, are uniformly mixed, grinding mashing, the sieving of 60 mesh
Afterwards with salt acid for adjusting pH to 3.0,45 DEG C of insulations obtain rapeseed dregs slurry for 2 hours；
B, starched to step A gained rapeseed dregs in add acid pectase, the addition of the acid pectase is rapeseed dregs
Slurry quality 0.1%, 45 DEG C insulation 3 hours after with potassium hydroxide adjust reactor in slurries Ph to 4.5；Add phytase, institute
It is the 0.7% of rapeseed dregs slurry quality to state the addition of phytase, and 50 DEG C are incubated 1 hour, then boiling water bath 20 minutes, end temperature
It is down to 50 DEG C of insulations standby；AMS is added, the addition of the AMS is the 0.3% of rapeseed dregs slurry quality, 50 DEG C
Insulation adds acid protease after 1 hour, the addition of the acid protease is the 1% of rapeseed dregs slurry quality, 50 DEG C of guarantors
Temperature 3 hours, then adjusts reactor slurries Ph to 7.0 with potassium hydroxide；Add neutral proteinase, the neutral proteinase plus
It is the 2% of rapeseed dregs slurry quality to enter amount, and 50 DEG C are incubated 3 hours, adds potassium hydroxide regulation reactor slurries Ph to 10.0；Plus
Enter alkali protease, the addition of the alkali protease is the 1% of rapeseed dregs slurry quality, and 50 DEG C are incubated 3 hours, add dilute salt
Acid regulation reactor slurries Ph to 4.2；
C, by the centrifugation of step B gained reactor slurries, filtering, obtain plant source amino acid polypeptide Water soluble fertilizer.The gained of embodiment 3
Amino acid classes and content such as table 3 in plant source amino acid polypeptide Water soluble fertilizer.
Table 3
Claims (1)
1. a kind of preparation method of plant source amino acid polypeptide Water soluble fertilizer, it is characterised in that：Comprise the following steps：
A, in mass ratio 1:4 weigh plant granulated slag and water, load reactor, are uniformly mixed, grinding mashing, after the sieving of 60 mesh
With salt acid for adjusting pH to 3.0,45 DEG C of insulations obtain Plant Powder slurry in 2 hours；The plant granulated slag is dregs of beans, peanut meal, rapeseed dregs
In one kind or their mixture；
B, to acid pectase is added in step A gained Plant Powder slurries, the addition of the acid pectase is plant granulated slag
Slurry quality 0.1%, 45 DEG C insulation 3 hours after with potassium hydroxide adjust reactor in slurries pH to 4.5；Add phytase, institute
It is the 0.7% of Plant Powder slurry quality to state the addition of phytase, and 50 DEG C are incubated 1 hour, then boiling water bath 20 minutes, last temperature
It is standby that degree is down to 50 DEG C of insulations；AMS is added, the addition of the AMS is the 0.3% of Plant Powder slurry quality,
50 DEG C of insulations add acid protease after 1 hour, and the addition of the acid protease is the 1% of Plant Powder slurry quality,
50 DEG C are incubated 3 hours, then adjust reactor slurries pH to 7.0 with potassium hydroxide；Add neutral proteinase, the neutral protein
The addition of enzyme is the 2% of Plant Powder slurry quality, and 50 DEG C are incubated 3 hours, adds potassium hydroxide regulation reactor slurries pH
To 10.0；Alkali protease is added, the addition of the alkali protease is the 1% of Plant Powder slurry quality, and 50 DEG C of insulations 3 are small
When, add watery hydrochloric acid regulation reactor slurries pH to 4.2；
C, by the centrifugation of step B gained reactor slurries, filtering, obtain plant source amino acid polypeptide Water soluble fertilizer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710122758.6A CN106831003A (en) | 2017-03-03 | 2017-03-03 | A kind of preparation method of plant source amino acid polypeptide Water soluble fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710122758.6A CN106831003A (en) | 2017-03-03 | 2017-03-03 | A kind of preparation method of plant source amino acid polypeptide Water soluble fertilizer |
Publications (1)
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107746319A (en) * | 2017-10-16 | 2018-03-02 | 昌乐县子农果蔬专业合作社 | A kind of water-soluble organic fertilizer and its application in capsicum plantation |
| CN113999071A (en) * | 2021-12-09 | 2022-02-01 | 中化农业（临沂）研发中心有限公司 | Growth-promoting stress-resistant quality-improving amino acid water-soluble fertilizer and preparation method and application thereof |
| CN115417713A (en) * | 2022-07-09 | 2022-12-02 | 襄阳维恩生物科技有限公司 | Production method of polypeptide fertilizer |
| CN118355829A (en) * | 2024-03-19 | 2024-07-19 | 广西宇花灵科技发展有限公司 | Passion fruit main vine fruiting cultivation method |
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| JPS5742591A (en) * | 1980-08-22 | 1982-03-10 | Fukuei Hiriyou Kk | Manufacture of fertilizer |
| CN101974576A (en) * | 2010-10-27 | 2011-02-16 | 江苏大学 | Method for producing salt-free compound amino acid powder from combination of sesame seed meal and soybean meal |
| CN102115774A (en) * | 2010-11-30 | 2011-07-06 | 华东理工大学 | Method for preparing plant polypeptide by enzyme process |
| CN104012775A (en) * | 2014-06-27 | 2014-09-03 | 成都美溢德生物技术有限公司 | Method for hydrolyzing vegetable protein |
| CN105884405A (en) * | 2015-11-03 | 2016-08-24 | 鹤壁市禾盛生物科技有限公司 | Preparation method of amino acid-polypeptide liquid fertilizer |
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| JPS5742591A (en) * | 1980-08-22 | 1982-03-10 | Fukuei Hiriyou Kk | Manufacture of fertilizer |
| CN101974576A (en) * | 2010-10-27 | 2011-02-16 | 江苏大学 | Method for producing salt-free compound amino acid powder from combination of sesame seed meal and soybean meal |
| CN102115774A (en) * | 2010-11-30 | 2011-07-06 | 华东理工大学 | Method for preparing plant polypeptide by enzyme process |
| CN104012775A (en) * | 2014-06-27 | 2014-09-03 | 成都美溢德生物技术有限公司 | Method for hydrolyzing vegetable protein |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107746319A (en) * | 2017-10-16 | 2018-03-02 | 昌乐县子农果蔬专业合作社 | A kind of water-soluble organic fertilizer and its application in capsicum plantation |
| CN113999071A (en) * | 2021-12-09 | 2022-02-01 | 中化农业（临沂）研发中心有限公司 | Growth-promoting stress-resistant quality-improving amino acid water-soluble fertilizer and preparation method and application thereof |
| CN115417713A (en) * | 2022-07-09 | 2022-12-02 | 襄阳维恩生物科技有限公司 | Production method of polypeptide fertilizer |
| CN118355829A (en) * | 2024-03-19 | 2024-07-19 | 广西宇花灵科技发展有限公司 | Passion fruit main vine fruiting cultivation method |
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Application publication date: 20170613 |