CN106819487A - A kind of low albumen mixed feed of piglet containing clostridium butyricum and application - Google Patents
A kind of low albumen mixed feed of piglet containing clostridium butyricum and application Download PDFInfo
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- CN106819487A CN106819487A CN201710139885.7A CN201710139885A CN106819487A CN 106819487 A CN106819487 A CN 106819487A CN 201710139885 A CN201710139885 A CN 201710139885A CN 106819487 A CN106819487 A CN 106819487A
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- piglet
- clostridium butyricum
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- 241000193171 Clostridium butyricum Species 0.000 title claims abstract description 40
- 206010012735 Diarrhoea Diseases 0.000 claims abstract description 22
- 239000000843 powder Substances 0.000 claims abstract description 18
- 240000008042 Zea mays Species 0.000 claims abstract description 13
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 13
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 13
- 235000005822 corn Nutrition 0.000 claims abstract description 13
- 241000894006 Bacteria Species 0.000 claims abstract description 12
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 12
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 12
- 235000019733 Fish meal Nutrition 0.000 claims abstract description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 10
- 239000004467 fishmeal Substances 0.000 claims abstract description 10
- 239000008103 glucose Substances 0.000 claims abstract description 10
- 239000005862 Whey Substances 0.000 claims abstract description 9
- 102000007544 Whey Proteins Human genes 0.000 claims abstract description 9
- 108010046377 Whey Proteins Proteins 0.000 claims abstract description 9
- 235000013312 flour Nutrition 0.000 claims abstract description 9
- 229930182817 methionine Natural products 0.000 claims abstract description 9
- 235000006109 methionine Nutrition 0.000 claims abstract description 9
- 150000003839 salts Chemical class 0.000 claims abstract description 9
- 239000004575 stone Substances 0.000 claims abstract description 9
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- 244000068988 Glycine max Species 0.000 claims abstract description 8
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 8
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims abstract description 8
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000004470 DL Methionine Substances 0.000 claims abstract description 7
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- FFEARJCKVFRZRR-UHFFFAOYSA-N methionine Chemical compound CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000002994 raw material Substances 0.000 claims description 13
- 235000008521 threonine Nutrition 0.000 claims description 8
- 239000004472 Lysine Substances 0.000 claims description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 2
- 201000009277 hairy cell leukemia Diseases 0.000 claims description 2
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- 230000000694 effects Effects 0.000 abstract description 4
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- 238000002474 experimental method Methods 0.000 description 33
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- 238000012360 testing method Methods 0.000 description 15
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- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 6
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- 229910052802 copper Inorganic materials 0.000 description 6
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- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 6
- 229910052742 iron Inorganic materials 0.000 description 6
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 6
- 235000001968 nicotinic acid Nutrition 0.000 description 6
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
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- 210000001198 duodenum Anatomy 0.000 description 3
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- 239000007858 starting material Substances 0.000 description 3
- 230000001228 trophic effect Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 235000014590 basal diet Nutrition 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
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- 230000036541 health Effects 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 210000000936 intestine Anatomy 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 235000014655 lactic acid Nutrition 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 235000011007 phosphoric acid Nutrition 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 208000003643 Callosities Diseases 0.000 description 1
- 241000193403 Clostridium Species 0.000 description 1
- 108010010256 Dietary Proteins Proteins 0.000 description 1
- 102000015781 Dietary Proteins Human genes 0.000 description 1
- -1 Dimethyl diaminophenazine chloride Chemical compound 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
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- 230000008901 benefit Effects 0.000 description 1
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- 230000037396 body weight Effects 0.000 description 1
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- 229940099352 cholate Drugs 0.000 description 1
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 1
- 235000015165 citric acid Nutrition 0.000 description 1
- 239000006027 corn-soybean meal Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000021245 dietary protein Nutrition 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
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- 235000011087 fumaric acid Nutrition 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 230000003862 health status Effects 0.000 description 1
- 235000020256 human milk Nutrition 0.000 description 1
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- 230000008676 import Effects 0.000 description 1
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- 231100000567 intoxicating Toxicity 0.000 description 1
- 230000002673 intoxicating effect Effects 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 210000004731 jugular vein Anatomy 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
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- 231100000572 poisoning Toxicity 0.000 description 1
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- 229920000136 polysorbate Polymers 0.000 description 1
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- 210000000664 rectum Anatomy 0.000 description 1
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- 238000012216 screening Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
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- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
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- 231100000167 toxic agent Toxicity 0.000 description 1
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- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Fodder In General (AREA)
Abstract
The present invention discloses a kind of low albumen mixed feed of piglet containing clostridium butyricum and its application.Including corn 60 65, dregs of beans 15 22, whey powder 36, fish meal 39, wheat bran 16, glucose 13, soya-bean oil 13, acidulant 0.1 0.5, calcium monohydrogen phosphate 0.5 1.2, stone flour 0.5 1.1, salt 0.1 0.5, L FE-5s 0.1 0.7, DL methionine 0.1 0.7, threonine 0.05 0.3, tryptophan 0.1 0.8, piglet premix 14, clostridium butyricum 0.001 0.005（Viable bacteria content is 1 × 10 in freeze-dried powder9CFU/g）.Experimental result shows：The clostridium butyricum additive of proper proportion is added in piglet low-protein feed has the effect for significantly improving piglet growth performance, reducing grice diarrhoea rate, improve intestinal health.
Description
Technical field
The invention belongs to farm animals nutrition technique research field, it is related to a kind of Low protein diet containing clostridium butyricum to match somebody with somebody
Side, is in particular a kind of application for improving the aspects such as piglet growth performance and intestinal health.
Background technology
Weaned piglet is an important stage in Swine Production, and ablactation stress is that the maximum after piglet is born through receiving should
Swash.Weanling pig is not perfect enough with developing immune system due to digesting, and diet form will by the transformation of breast milk to solid feed
It is influenceed to digest and assimilate nutriment.The nutriment do not digested and assimilated draws in enteron aisle as the fermentation substrate of bacterium
Pathogen propagation is played, substantial amounts of toxicant is produced, so as to cause diarrhoea, intestinal health is damaged.And fed after weaned piglet high
Protein feeds easily causes function of intestinal canal disorderly, and then causes diarrhoea.
Antibioticses growth promoter is added in pig starter feed can effectively reduce Incidence of Diarrhea, but antibioticses are given birth to
Accelerator long is a large amount of using causing problems to occur in feed industry, and such as pathogen produces drug resistance, animal immune machine
Medicament residue in energy decline, internal flora imbalance, animal products etc..Therefore the nutrition of piglet growth performance and intestinal health is adjusted
The novelty for controlling this field awaits improving.This promotes researcher to find control weaning period diarrhoea, maintain gut integrity
Alternative, wherein reduce dietary protein level to supplement synthesizing amino acid simultaneously is that to improve piglet growth performance, control young
The effective measures of pig Post Weaning Diarrhea.
Clostridium butyricum is one of popular Substitutes For Antibiotic, for piglet low-protein feed additive relevant report also
It is rare.Utilized we have studied one kind regulation pig starter feed by adding clostridium butyricum in low-protein amino acid diet
The mixed feed of rate and gut flora, the nutrition regulation of piglet growth performance and intestinal health is improved with this, can be in certain journey
Alleviate protein raw material on degree and lack problem, reduce feed cost, while reducing pollution of the plant to environment, reduce piglet
Diarrhoea, improves the economic benefit of aquaculture.
The content of the invention
It is an object of the invention to disclose a kind of low albumen mixed feed of piglet containing clostridium butyricum.
Another object of the present invention is to disclose prepared feed to adjust Growth Performance of Weaning Piglets and intestinal health
The application effect of section.
To achieve the above object, the invention discloses following technology contents
A kind of mixed feed for adjusting piglet growth performance and intestinal health, it is characterised in that protein contains in reducing feedstuff
Amount, while using clostridium butyricum feed addictive, carrying out reasonable preparation daily ration, is made up of the raw material of following portions by weight：
Corn 60-65 dregs of beans 15-22 whey powders 3-6
Fish meal 3-9 wheat bran 1-6 glucose 1-3
Soya-bean oil 1-3 acidulant 0.1-0.5 calcium monohydrogen phosphates 0.5-1.2
Stone flour 0.5-1.1 salt 0.1-0.5 L lysine HCLs 0.1-0.7
DL- METHIONINE 0.1-0.7 tryptophan 0.1-0.8 threonines 0.05-0.3
Piglet premix 1-4 clostridium butyricums 0.001-0.005（Viable bacteria content is 1 × 10 in freeze-dried powder9CFU/g）
Wherein described piglet premix refers to contain in every kilogram of premix：14,600 mg iron, 630 mg copper, 410 mg
Manganese, 10,400 mg zinc, 15,000 μ g iodine, 17,000 μ g selenium, 227,600 IU VA, 22,800 IU VD3, 1,100 mg VE,
30 mg VK3, 700 mg VB2, 200 mg VB6, 1,340 mg pantothenic acid, 2,300 mg nicotinic acid, 8 mg biotins.
Acidulant of the present invention refers to Shandong Tian Huiyuan bio tech ltd product, main component be lactic acid,
Orthophosphoric acid, citric acid, fumaric acid, formic acid.
The preparation method of Weanling pig compound feed of the present invention：Corn, two kinds of raw materials of dregs of beans are crushed, other are former
Material need not be crushed, and be weighed respectively according to part by weight, be mixed in multifunctional mini-mixer.
The preferred formula of the present invention one：
The whey powder 4 of 64.495 dregs of beans of corn 16.3
The glucose 1.5 of 3.8 wheat bran of fish meal 5
The salt 0.3 of 0.65 stone flour of soya-bean oil 1 acidulant, 0.4 calcium monohydrogen phosphate 0.6
The threonine 0.1 of 0.4 tryptophan of L lysine HCL 0.4DL- methionine 0.05
The clostridium butyricum 0.005 of piglet premix 1（Viable bacteria content is 1 × 10 in freeze-dried powder9CFU/g）
Wherein described piglet premix refers to contain in every kilogram of premix：14,600 mg iron, 630 mg copper, 410 mg
Manganese, 10,400 mg zinc, 15,000 μ g iodine, 17,000 μ g selenium, 227,600 IU VA, 22,800 IU VD3, 1,100 mg VE,
30 mg VK3, 700 mg VB2, 200 mg VB6, 1,340 mg pantothenic acid, 2,300 mg nicotinic acid, 8 mg biotins.
Presently preferred formula：
The whey powder 4.5 of 64.199 dregs of beans of corn 16.3
The glucose 2.5 of 4 wheat bran of fish meal 3.7
The salt 0.3 of 0.5 stone flour of soya-bean oil 1 acidulant, 0.4 calcium monohydrogen phosphate 0.6
The threonine 0.15 of 0.4 tryptophan of L lysine HCL 0.4DL- methionine 0.05
The clostridium butyricum 0.001 of piglet premix 1（Viable bacteria content is 1 × 10 in freeze-dried powder9CFU/g）
Wherein described piglet premix refers to contain in every kilogram of premix：14,600 mg iron, 630 mg copper, 410 mg
Manganese, 10,400 mg zinc, 15,000 μ g iodine, 17,000 μ g selenium, 227,600 IU VA, 22,800 IU VD3, 1,100 mg VE,
30 mg VK3, 700 mg VB2, 200 mg VB6, 1,340 mg pantothenic acid, 2,300 mg nicotinic acid, 8 mg biotins.
The present invention further discloses the feed prepared to the application effect of weanling pig intestinal microflora.Especially
It is the application to improving the aspect such as diarrhea of weaned piglets and gut flora balance.
Weanling pig compound feed of the invention test result indicate that：
Experiment C groups, experiment D groups and experiment E groups are significantly（P ＜ 0.05）Grice diarrhoea rate and feed-weight ratio are reduced, significantly（P ＜
0.05）Improve average daily gain；The quantity of experiment C groups and experiment D groups jejunum and ileum Bacillus acidi lactici is notable（P ＜ 0.05）It is higher than
Experiment B groups, and the quantity for testing Escherichia coli in C groups and experiment D group ileums is significantly reduced（P ＜ 0.05）.In order to further
Illustrate the positive effect of the present invention, there is provided following test method and result：
1. experimental animal and packet：
Designed using single factor experiment, the healthy Du × length × Dasanyuan hybridization wean for choosing the kg of body weight 6.78 ± 0.51 is young
Pig 120, is randomly divided into 5 treatment, and each treatment is divided into 4 repetitions, and each repeats 6 pigs.Each treatment group feeds respectively
Different daily rations：Feeding basal diet (NC), feeding Low-protein diet, the feeding clostridium butyricum of Low-protein diet+0.001（Concentration is
1×105CFU/g）, feeding the clostridium butyricum of Low-protein diet+0.005（Concentration is 5 × 105CFU/g）Group, feeding Low-protein diet
+ 0.009 clostridium butyricum（Concentration is 9 × 105CFU/g）.
2. Screening Experiment of Diet and trophic level：
According to NRC（1998）Prepare pig basal diet formula and trophic level is as shown in table 1.
The Basic drawing of table 1 is constituted and trophic level（Feeding basis）
Contain in every kilogram of premixes of a：14,600 mg iron, 630 mg copper, 410 mg manganese, 10,400 mg zinc, 15,000 μ g
Iodine, 17,000 μ g selenium, 227,600 IU VA, 22,800 IU VD3,1,100 mg VE, 30 mg VK3,700 mg VB2,
200 mg VB6,1,340 mg pantothenic acid, 2,300 mg nicotinic acid, 8 mg biotins.
B is according to NRC（1998）Feed ingredient meter is calculated.
3. testing regulations：
Experiment is carried out on ten thousand pig farms of a commodity, 28 days experimental periods.Feeding, management, immune carried out by pig farm established procedure.Raise
After feeding 28 days, 1 weanling pig is randomly selected from each repeating groups that each is processed and is butchered, cutd open and kill preceding 12h fasting, then adopted
Put to death with the method for jugular vein bloodletting, open abdominal cavity, ligation spray door valve, pyloric valve, rectum distal end, aseptic to take 12 fingers immediately
The contents such as intestines, jejunum, ileum, after being placed in liquid nitrogen flash freezer, relay and freeze with to be analyzed in -80 DEG C of refrigerators.Entirely tested
The health status of piglet is observed in journey, record is dead, wash in a pan piglet and diarrhoea, medicining condition.
4. testing index：
The measure of growth performance：Claim individual weight, ramming material respectively at 28 d, 56 d of experiment, calculate ADG, ADFI and F/G.
The measure of diarrhea rate：Within whole experimental period, two heads of the generation diarrhoea piglet for the treatment of group are counted respectively daily
Number, off-test calculates every group of diarrhea rate.Diarrhoea standards of grading are shown in Table 2.
The diarrhoea standards of grading of table 2
Note：Scours index standards of grading are with reference to Kelly（1990）The method of offer is carried out.
The measure of gut flora：
5. statistical analysis：
Variance analysis is carried out using the One-Way ANOVA in the Compare Means modules under SAS statistical softwares, and is used
Duncan ' s Multiple range tests carry out group difference significance test, P<0.05 is significant difference.
Application effect of the low albumen mixed feed disclosed by the invention in weanling pig feed, mainly includes：
（1）Serum Indexes：
In table 3, diet group based on A, B is Low-protein diet group, and C is the clostridium butyricum of Low-protein diet+0.001（Concentration is 1
×105CFU/g）Group, D is the clostridium butyricum of Low-protein diet+0.005（Concentration is 5 × 105CFU/g）Group, E is Low-protein diet
+ 0.009 clostridium butyricum（Concentration is 9 × 105CFU/g）Group.
Influence of the table 3 to piglet growth performance
As shown in Table 3,1. experiment B groups are not notable with body counterpoise, opisthosoma counterpoise, ADG, ADFI and F/G difference at the beginning of experiment A groups（P
＞ 0.05）；Therefore, match somebody with somebody Low-protein diet rationally, meeting piglet growth needs, the premise of piglet growth performance is not influenceed
Under, save cost.
2. the feed-weight ratio of C groups is tested（F/G）A groups and experiment B groups are substantially less than tested, therefore, experiment C groups addition is to add
Plus lower limit.
3. the feed-weight ratio of D groups is tested（F/G）C groups and experiment E groups are substantially less than tested, therefore, experiment D groups addition is to add
Plus limit.
4. therefore, the addition of clostridium butyricum additive is in the range of 0.001-0.005.
（2）Diarrhea rate：
In table 4, diet group based on A, B is Low-protein diet group, and C is the clostridium butyricum of Low-protein diet+0.001（Concentration is 1
×105CFU/g）Group, D is the clostridium butyricum of Low-protein diet+0.005（Concentration is 5 × 105CFU/g）Group, E is Low-protein diet
+ 0.009 clostridium butyricum（Concentration is 9 × 105CFU/g）Group.
Influence of the Low-protein diet of table 4 to diarrhea of weaned piglets rate
Note：Shoulder note between colleague, represents that difference is not notable without letter（P ＞ 0.05）, letter is different to represent significant difference（P ＜
0.05）.
As shown in Table 4,1. Low-protein diet B groups can significantly reduce grice diarrhoea rate；
2. C, D, E test group of clostridium butyricum be with the addition of compared with B groups, grice diarrhoea rate is significantly reduced, but experiment C, D, E group it
Between difference it is not notable.
3. experiment E compositions are originally higher than experiment C groups and experiment D groups.Therefore, in Low-protein diet clostridium butyricum addition
It is that 0.001-0.005 ratios are convenient.
（3）Gut flora：
In table 5, B is Low-protein diet group, and C is the clostridium butyricum of Low-protein diet+0.001（Concentration is 1 × 105CFU/g）Group, D
It is the clostridium butyricum of Low-protein diet+0.005（Concentration is 5 × 105CFU/g）Group.
Influence (lg CFU/g) of the clostridium butyricum of table 5 to intestine of young pigs microorganism
Shown by the result of the test of table 5：
1. the Bacillus acidi lactici and E. CoIi content in duodenum be not notable in each experiment group difference（P ＞ 0.05）.
2. Bacillus acidi lactici digital display writes in testing C groups and experiment D group jejunums（P ＜ 0.05）Higher than experiment B groups.Coliform count
Difference is not notable between measuring three groups（P ＞ 0.05）.
3. the Bacillus acidi lactici and Escherichia coli quantity in experiment C groups and experiment D group ileums are notable（P ＜ 0.05）Higher than experiment
B groups.Bacillus acidi lactici and Escherichia coli quantity difference in experiment C groups and experiment D group ileums be not notable（P ＞ 0.05）.But experiment D
The Bacillus acidi lactici quantity of group improves 1.73% than experiment C groups, and the Escherichia coli quantity for testing D groups is had a competition and tests C groups and reduce
2.99%。
4. therefore, the addition of clostridium butyricum additive is in the range of 0.001-0.005.
The present invention has carried out safety experiment to the Low protein diet formula with clostridium butyricum as feed addictive, its method
It is as follows：
（1）Step：
Material and method：Du × length × big piglet 6 that 28 ages in days wean is selected, clinical health, development are normal, experimental period 28
My god, poisoning manifestations and death condition are observed in experimental period at any time.
（2）The pig starter feed raw material composition of feeding：
Corn 63.54, dregs of beans 16.5, whey powder 4, fish meal 4.4, wheat bran 4.2, glucose 2, soya-bean oil 1, acidulant 0.3, phosphoric acid
Hydrogen calcium 0.5, stone flour 0.6, salt 0.35, L lysine HCL 0.2, DL- METHIONINE 0.25, tryptophan 0.05, threonine
0.1st, piglet premix 2, clostridium butyricum 0.01（Viable bacteria content is 1 × 10 in freeze-dried powder9CFU/g）.
As a result：Above-mentioned experiment daily ration is fed to weanling pig, continuous feeding 28 days has no obvious intoxicating phenomenon, observation period
It is interior without death, each main organs show no obvious abnormalities in postmortem.
（3）Conclusion：0.01% clostridium butyricum is added in weanling pig daily ration（Viable bacteria content is 1 × 10 in freeze-dried powder9CFU/
g）Feeding piglet, feeding effect safety.
Specific embodiment
Make a step to the present invention by following examples to illustrate, but not as limitation of the invention.Embodiments of the invention
Described in parts by weight raw material refer to gram, kilogram or ton, can be according to being adjusted the need for produce reality.It is of the invention used
The various raw materials for arriving are for example：Calcium monohydrogen phosphate, stone flour, salt, L lysine HCL, DL- METHIONINE, tryptophan, threonine, fourth
Sour clostridium is externally on sale；Wherein following product is commercially available：
Acidulant refers to Shandong Tian Huiyuan bio tech ltd product；
Piglet premix refers to Tianjin Ding Sheng feed corporation,Ltds product；
Corn refers to common commercially available two grades of corns；
Dregs of beans refers to Cofco Group Co., Ltd.'s product；
Whey powder and glucose refer to Tianjin ascension feed corporation,Ltd product；
Fish meal refers to import peru fish meal；
Wheat bran refers to Hebei Yongsheng Foodstuff Co., Ltd.'s product；
Clostridium butyricum refers to Luoyang Rui Lai bioengineering Co., Ltd product.
Embodiment 1
The low albumen mixed feed raw material composition of weanling pig：
Corn 63.199, dregs of beans 17.3, whey powder 4.5, fish meal 4, wheat bran 3.7, glucose 2.5, soya-bean oil 1, acidulant 0.4,
Calcium monohydrogen phosphate 0.5, stone flour 0.6, salt 0.3, L lysine HCL 0.4, DL- METHIONINE 0.4, threonine 0.15, piglet are pre-
Batch mixing 1, clostridium butyricum 0.001（Viable bacteria content is 1 × 10 in freeze-dried powder9CFU/g）.
Wherein described piglet premix refers to contain in every kilogram of premix：14,600 mg iron, 630 mg copper, 410
Mg manganese, 10,400 mg zinc, 15,000 μ g iodine, 17,000 μ g selenium, 227,600 IU VA, 22,800 IU VD3, 1,100 mg
VE, 30 mg VK3, 700 mg VB2, 200 mg VB6, 1,340 mg pantothenic acid, 2,300 mg nicotinic acid, 8 mg biotins.
Preparation method：Corn, two kinds of raw materials of dregs of beans are crushed, other raw materials need not be crushed, according to part by weight point
Do not weighed, mixed in multifunctional mini-mixer.
Embodiment 2
The low albumen mixed feed raw material composition of weanling pig：
Corn 64.495, dregs of beans 16.8, whey powder 4, fish meal 3.3, wheat bran 4.5, glucose 2, soya-bean oil 1, acidulant 0.4, phosphorus
Sour hydrogen calcium 0.65, stone flour 0.6, salt 0.3, L lysine HCL 0.4, DL- METHIONINE 0.4, tryptophan 0.05, threonine
0.1st, piglet premix 1, clostridium butyricum 0.005（Viable bacteria content is 1 × 10 in freeze-dried powder9CFU/g）.
Wherein described piglet premix refers to contain in every kilogram of premix：14,600 mg iron, 630 mg copper, 410
Mg manganese, 10,400 mg zinc, 15,000 μ g iodine, 17,000 μ g selenium, 227,600 IU VA, 22,800 IU VD3, 1,100 mg
VE, 30 mg VK3, 700 mg VB2, 200 mg VB6, 1,340 mg pantothenic acid, 2,300 mg nicotinic acid, 8 mg biotins.
Preparation method：Corn, two kinds of raw materials of dregs of beans are crushed, other raw materials need not be crushed, according to part by weight point
Do not weighed, mixed in multifunctional mini-mixer.
Embodiment 3
The method that weanling pig is fed using the low albumen mixed feed of weanling pig：
This series formula is applicable 28 age in days weanling pigs, and working life is 28 ~ 40 days after wean, according to circumstances to allow piglet free choice feeding
, it is necessary to sufficient drinking-water is provided, other are carried out by normal feeding and management.
Embodiment 4
Comparative test
Conventional weanling pig feed：
Method：Corn-soybean meal weanling pig daily ration is prepared, without clostridium butyricum.Select 28 age in days, 6 piglets, experimental period
28 days.
Step：3 piglets are butchered after off-test, takes duodenum, jejunum, ileum intestinal segment contents samples 1
G, is dissolved in the SPSS of 9 mL, is sufficiently mixed, then step by step 10 times be serially diluted to 10-7.Selection 10-5-10-7
Dilution factor, taking during 0.1 mL dilutions are inoculated into corresponding rolling pipe culture medium carries out Bacillus acidi lactici culture.Choose 10-2-10-5
Dilution factor, take 0.2 mL dilutions carries out the counting of Escherichia coli in corresponding plating medium.
E. coli counts use maconkey agar culture medium：Peptone, 20.0 g/L；Lactose, 10.0g/L；Cholate,
5.0 g/L；Sodium chloride, 5.0 g/L；Dimethyl diaminophenazine chloride, 0.075 g/L；Agar, 12.0 g/L；pH 7.4 ± 0.2.
Bacillus acidi lactici is counted and uses Rogosa agar：Tryptone, 10.0 g/L；Yeast extract, 5.0 g/L；Glucose,
20.0 g/L；Tween 80,1.0 mL/L；Potassium dihydrogen phosphate, 6.0 g/L；Ammonium citrate, 2.0 g/L；Sodium acetate, 17.0 g/L；
Magnesium sulfate, 0.575 g/L；Manganese sulfate, 0.12 g/L；Ferrous sulfate, 0.034 g/L；Agar, 20.0 g/L；pH 5.4 ±
0.2。
The agar medium of wherein Bacillus acidi lactici is made Hungates rolling pipes；Escherichia coli use flat board culture.It is all of
Culture medium is placed in 37 DEG C of incubators, is counted after 24~48 h of culture.Each dilution factor does two flat boards or rolling pipe, with 30
The flat board of~300 bacterium colonies or the dilution factor of rolling pipe do counting and use.
As a result：Bacillus acidi lactici and E. CoIi content are respectively 5.16lg CFU/g and 3.91 lg CFU/ in duodenum
g；Bacillus acidi lactici and E. CoIi content are respectively 6.33 lg CFU/g and 3.55 lg CFU/g in jejunum；Lactic acid bar in ileum
Bacterium and E. CoIi content are respectively 6.57 lg CFU/g and 3.93 lg CFU/g.
Conclusion：Illustrated by comparative test：Clostridium butyricum is added in Low-protein diet can adjust Intestinal Microflora of Weanling Piglets,
Improve intestinal health and improve piglet holistic health.
Claims (3)
1. the low albumen mixed feed of a kind of piglet containing clostridium butyricum, it is characterised in that be made up of the raw material of following portions by weight：
Corn 60-65 dregs of beans 15-22 whey powders 3-6
Fish meal 3-9 wheat bran 1-6 glucose 1-3
Soya-bean oil 1-3 acidulant 0.1-0.5 calcium monohydrogen phosphates 0.5-1.2
Stone flour 0.5-1.1 salt 0.1-0.5 L lysine HCLs 0.1-0.7
DL- METHIONINE 0.1-0.7 tryptophan 0.1-0.8 threonines 0.05-0.3
Piglet premix 1-4 clostridium butyricums 0.001-0.005（Viable bacteria content is 1 × 10 in freeze-dried powder9CFU/g）.
2. a kind of low albumen mixed feed of piglet containing clostridium butyricum described in claim 1 is improving diarrhea of weaned piglets and enteron aisle
Application in terms of colony balance.
3. the application described in claim 2, wherein described improvement diarrhea of weaned piglets and gut flora balance are referred to：Wean
Application in terms of grice diarrhoea rate, average daily gain, daily gain, feed-weight ratio and gut flora.
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Application publication date: 20170613 |