CN106745814A - Compound improver of water quality for aquaculture and preparation method thereof - Google Patents
Compound improver of water quality for aquaculture and preparation method thereof Download PDFInfo
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- CN106745814A CN106745814A CN201611268721.6A CN201611268721A CN106745814A CN 106745814 A CN106745814 A CN 106745814A CN 201611268721 A CN201611268721 A CN 201611268721A CN 106745814 A CN106745814 A CN 106745814A
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 73
- 238000009360 aquaculture Methods 0.000 title claims abstract description 25
- 244000144974 aquaculture Species 0.000 title claims abstract description 25
- 150000001875 compounds Chemical class 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims description 10
- 241000894006 Bacteria Species 0.000 claims abstract description 36
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 24
- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 claims abstract description 9
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000000440 bentonite Substances 0.000 claims abstract description 9
- 229910000278 bentonite Inorganic materials 0.000 claims abstract description 9
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000002509 fulvic acid Substances 0.000 claims abstract description 9
- 229940095100 fulvic acid Drugs 0.000 claims abstract description 9
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims abstract description 8
- 239000011734 sodium Substances 0.000 claims abstract description 8
- 229910052708 sodium Inorganic materials 0.000 claims abstract description 8
- RKFAZBXYICVSKP-AATRIKPKSA-N alpha-asarone Chemical compound COC1=CC(OC)=C(\C=C\C)C=C1OC RKFAZBXYICVSKP-AATRIKPKSA-N 0.000 claims abstract description 6
- 239000001963 growth medium Substances 0.000 claims description 13
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 8
- 229940041514 candida albicans extract Drugs 0.000 claims description 7
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 claims description 7
- 239000012138 yeast extract Substances 0.000 claims description 7
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 claims description 6
- 235000019345 sodium thiosulphate Nutrition 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 5
- 108090000623 proteins and genes Proteins 0.000 claims description 5
- 102000004169 proteins and genes Human genes 0.000 claims description 5
- KZJWDPNRJALLNS-VJSFXXLFSA-N sitosterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CC[C@@H](CC)C(C)C)[C@@]1(C)CC2 KZJWDPNRJALLNS-VJSFXXLFSA-N 0.000 claims description 5
- 229920001817 Agar Polymers 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 4
- 108010080698 Peptones Proteins 0.000 claims description 4
- 241000238370 Sepia Species 0.000 claims description 4
- 239000008272 agar Substances 0.000 claims description 4
- 239000001110 calcium chloride Substances 0.000 claims description 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 239000012153 distilled water Substances 0.000 claims description 4
- 238000005286 illumination Methods 0.000 claims description 4
- 239000002054 inoculum Substances 0.000 claims description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 4
- 235000019319 peptone Nutrition 0.000 claims description 4
- 239000003755 preservative agent Substances 0.000 claims description 4
- 230000002335 preservative effect Effects 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 3
- 239000011435 rock Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 2
- 230000006872 improvement Effects 0.000 abstract description 6
- 244000005700 microbiome Species 0.000 abstract description 3
- 230000029553 photosynthesis Effects 0.000 abstract description 2
- 238000010672 photosynthesis Methods 0.000 abstract description 2
- 238000000746 purification Methods 0.000 abstract description 2
- 238000009395 breeding Methods 0.000 description 7
- 230000001488 breeding effect Effects 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 239000005416 organic matter Substances 0.000 description 5
- 239000000758 substrate Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- 241000251468 Actinopterygii Species 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 238000009825 accumulation Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 230000000050 nutritive effect Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- FENRSEGZMITUEF-ATTCVCFYSA-E [Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].OP(=O)([O-])O[C@@H]1[C@@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H]1OP(=O)([O-])[O-] Chemical compound [Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].OP(=O)([O-])O[C@@H]1[C@@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H]1OP(=O)([O-])[O-] FENRSEGZMITUEF-ATTCVCFYSA-E 0.000 description 1
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 229940083982 sodium phytate Drugs 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L sodium sulphate Substances [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- -1 thio Sodium sulphate Chemical compound 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of compound improver of water quality for aquaculture, it is made up of the component of following weight percentage:50~60 parts of bentonite, 0.01 0.1 parts of asarone, 0.01 0.1 parts of Sitosterolum, 20~25 parts of sodium humate, 10~15 parts of fulvic acid, 10~15 parts of photosynthetic bacteria.The present invention has good water body improvement, lifting water body self-purification ability, improves the ecological environment, and promotes microorganism photosynthesis.
Description
Technical field
The present invention relates to a kind of modifying agent for aquaculture and preparation method thereof, aquatic products to be used for more particularly, to one kind
Compound improver of water quality of cultivation and preparation method thereof.
Background technology
With being continuously increased for China's pond culture area, ecologic structure and the tradition breeding way of aquaculture itself
Defect so that culture fishery has many outstanding problems, such as main breed variety is in a bad way and break out and spread;Abuse
Fishing medicine causes ecology fragility, and aquatic products edible safety declines;With the continuous expansion of interior pool cultured area, breeding wastewater discharge
And piled up by the organic sludge that residual bait, excrement, biological remains are formed, breeding water body organic contamination is constantly aggravated, breeding ecological
Environmental quality deteriorates etc. and to have turned into the environment and ecological problem for enjoying public concern year by year.In aquaculture process, pond
The situation of substrate can influence breeding water body environment, and substrate and water quality collectively form living space and the water body material energy of aquatile
Measure the carrier space of circulation.Mass exchange between substrate and water body is the main source of its nutritive salt, by the friendship of nutritive salt
Change and material process recycling, bed mud turn into be grown in one of source of nutritive salt needed for the aquatic animals and plants of pond bottom.Though
Mostly without direct relation between the soil or deposit and aquatic animal biomass of right water bottom, but water body substrate condition pair
The influence of water quality is larger, thus also can produce particularly important influence to the existence of fish and other aquatic animals and growth.And
The existing improver of water quality for aquaculture is mostly relatively simple, and effect is not fine.Some microorganism formulations and nutrition
Although accelerator has certain repair to water quality, can but cause overlying water nitrate nitrogen and ammoniacal nitrogen to rise, promote floating
Trip algae is interim to be grown.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of with good improvement, lifting water body self-purification ability
, improve the ecological environment, promote the photosynthetic compound improver of water quality and simple to operate, valency for aquaculture of microorganism
The cheap preparation method of lattice.
The present invention solve the technical scheme that is used of above-mentioned technical problem for:
A kind of compound improver of water quality for aquaculture with good improvement, modifying agent is by following weight percentage
Component composition:50~60 parts of bentonite, asarone 0.01-0.1 parts, cupreol 0.01-0.1 parts, sodium humate 20~25
Part, 10~15 parts of fulvic acid, 10~15 parts of photosynthetic bacteria.Above-mentioned is a kind of simple to operate, cheap for aquaculture
Compound improver of water quality preparation method, comprise the following steps:
Photosynthetic bacteria culture medium and condition of culture:Using yeast extract, protein culture medium, basic recipe is:
CaCl20.3g, MgSO4·7H2O0 5g, yeast extract 3g, peptone 3g, distilled water 1000ml, pH:6.8.Such as need solid processed
Culture medium adds 2 parts of agar again.Cultivation temperature:25 DEG C~30 DEG C optimal, intensity of illumination 2000LX~5000LX, PH:7.5~8.5
Most preferably;The culture of photosynthetic bacteria:Strain is inoculated into the culture medium of bacterium of having gone out, two grades of cultures is carried out in triangular flask, daily
Rock several times.Topped up with water in the reactor of 40L, breather pipe is put into, covering preservative film one, to reinstate hypochlorous acid sterilizing 24 small
When, use sodium thiosulfate(1L hypochlorous acid needs 150g sodium thiosulfate)To neutralize.In with 15 parts of inoculum concentration access reactor,
Ventilated with pump and cultivated, with the density of spectrophotometer tracking measurement bacterium solution, obtain bacterium solution density it is higher when, obtain photosynthetic bacteria standby
With;
Photosynthetic bacteria obtained above is mixed according to a certain percentage with bentonite, sodium humate, fulvic acid, 2 times of water are added, stirred
Mixing is mixed, 2h is adsorbed at a certain temperature, uniform temperature is 25 DEG C~30 DEG C.The paste of sepia is obtained, as water
Produce the compound improver of water quality of cultivation.
The invention has the advantages that:The compound improver of water quality for aquaculture that the present invention is obtained can improve foster
Water quality is grown, makes dissolved oxygen in water body(DO)Content can effectively improve, COD(CODMn)It is decreased obviously, ammonia
Nitrogen(NH4+-N)And nitrite nitrogen(NO2--N)Removal effect significantly, water pH value stabilization in water quality standard for fishery scope(It is light
Water 6.5~8.5);The accumulation of bottom material of pond for breeding organic matter can be to a certain extent reduced, while the improver of water quality is with low cost,
Preparation method is simple, easy to use in aquaculture process, works well, and will not produce secondary pollution when in use, has
There is stronger exploitability.
Specific embodiment
The present invention is described in further detail with reference to embodiments.
Embodiment 1:
A kind of compound improver of water quality for aquaculture with good improvement, modifying agent is by following weight percentage
Component composition:50~60 parts of bentonite, asarone 0.01-0.1 parts, cupreol 0.01-0.1 parts, sodium humate 20~25
Part, 10~15 parts of fulvic acid, 10~15 parts of photosynthetic bacteria, asarone and cupreol promote photosynthetic bacteria photosynthesis, so that
Its effect for producing hydrogen, fixed nitrogen and decomposing organic matter is improved, the purpose of water improvement is reached, meanwhile, promote photosynthetic bacteria synthesis bacterium
Body protein, not only improves ecological environment, also for aquaculture provides high-quality feedstuff.A kind of above-mentioned operation letter
Singly, the preparation method of the cheap compound improver of water quality for aquaculture, comprises the following steps:Photosynthetic bacteria is cultivated
Base and condition of culture:Using yeast extract, protein culture medium, basic recipe is:CaCl20.3g, MgSO4·7H2O0 5g, yeast
Cream 3g, peptone 3g, distilled water 1000ml, pH:6.8.Solid medium processed is such as needed to add 2 parts of agar again.Cultivation temperature:25 DEG C~
30 DEG C optimal, intensity of illumination 2000LX~5000LX, PH:7.5~8.5 is optimal;The culture of photosynthetic bacteria:Strain is inoculated into and is gone out
In the culture medium of good bacterium, two grades of cultures are carried out in triangular flask, rocked daily several times.Topped up with water in the reactor of 40L, it is put into
Breather pipe, covers preservative film one and reinstates hypochlorous acid sterilizing 24 hours, uses sodium thiosulfate(1L hypochlorous acid needs 150g thio
Sodium sulphate)To neutralize.In with 15 parts of inoculum concentration access reactor, ventilated with pump and cultivated, with spectrophotometer tracking measurement bacterium
The density of liquid, obtain bacterium solution density it is higher when, obtain photosynthetic bacteria standby;
Photosynthetic bacteria obtained above is mixed according to a certain percentage with bentonite, sodium humate, fulvic acid, 2 times of water are added, stirred
Mixing is mixed, 2h is adsorbed at a certain temperature, uniform temperature is 25 DEG C~30 DEG C.The paste of sepia is obtained, as water
Produce the compound improver of water quality of cultivation.The embodiment of the present invention 1 is used for the compound improver of water quality of aquaculture, by 15~22kg/
Hm2 is splashed to test tank, and water pH value can be maintained to be in the growth scope for being conducive to fish, and DO contents are in 5mg/ in keeping water body
More than L, is conducive to fish growth.
Embodiment 2:
A kind of compound improver of water quality for aquaculture, is made up of the component of following weight percentage:50 parts of bentonite, it is rotten
23 parts of sodium phytate, 12 parts of fulvic acid, 15 parts of photosynthetic bacteria.
Its preparation method is comprised the following steps:
(1)Photosynthetic bacteria culture medium and condition of culture:Using yeast extract, protein culture medium, basic recipe is:CaCl20.3g,
MgSO4·7H2O0.5g, yeast extract 3g, peptone 3g, distilled water 1000ml, pH:6.8.Solid medium processed is such as needed to add 2 parts again
Agar.Cultivation temperature:25 DEG C~30 DEG C optimal, intensity of illumination:2000LX~5000LX, PH:7.5~8.5 is optimal;
(2)The culture of photosynthetic bacteria:Strain is inoculated into the culture medium of bacterium of having gone out, two grades of cultures is carried out in triangular flask, often
It rocks several times.Topped up with water in the reactor of 40L, breather pipe is put into, covers preservative film one and reinstate hypochlorous acid sterilizing 24
Hour, use sodium thiosulfate(1L hypochlorous acid needs 150g sodium thiosulfate)To neutralize.Reactor is accessed with 15 parts of inoculum concentration
In, ventilated with pump and cultivated, with the density of spectrophotometer tracking measurement bacterium solution, obtain bacterium solution density it is higher when, obtain photosynthetic thin
Bacterium is standby;
(3)By step(2)The photosynthetic bacteria for obtaining is mixed with bentonite, sodium humate, fulvic acid by 15 parts, 50 parts, 23 parts, 12 parts
Close, add 2 times of water, stir and evenly mix, 2h is adsorbed at a certain temperature.Obtain the paste of sepia, the as embodiment of the present invention 2
For the compound improver of water quality of aquaculture.
The embodiment of the present invention 2 is used for the compound improver of water quality of aquaculture, is splashed to test tank by 15~22kg/hm2,
There is stronger degradation to water body COD and can continue until the 10th day, there is obvious removal to act on and sustainable to water body NO2--N
6d, can substantially reduce the accumulation of substrate organic matter.
The present invention is used for water quality improvement effect test for the compound improver of water quality of aquaculture:
1st, experiment is 0.67hm2, depth of water 1m with 2 mouthfuls of pond areas.Water, daily 11 are not changed during experiment:00 or so collection water
Sample and bed mud sample;
2nd, test method:Test group is used for the compound improver of water quality of aquaculture using the embodiment of the present invention 1, not use this
Compound improver of water quality is control group;
3rd, test effect:Improver of water quality of the present invention can significantly reduce cultivating pool Nitrite nitrogen, ammonia nitrogen and chemistry
Oxygen demand.Water quality can be improved, water body pH is in water quality standard for fishery scope for stabilization, effectively degraded water body organic matter, one
Determine the accumulation of reduction bottom material of pond for breeding organic matter in degree.
Specific embodiment described herein is only to explanation for example of the invention.The technical field of the invention
Technical staff can be made various modifications or supplement to described specific embodiment or be substituted using similar mode, but
Without departing from of the invention or surmount scope defined in appended claims.
Claims (4)
1. a kind of compound improver of water quality for aquaculture, it is characterised in that the modifying agent is by following weight percentage
Component composition:50~60 parts of bentonite, asarone 0.01-0.1 parts, cupreol 0.01-0.1 parts, sodium humate 20~25
Part, 10~15 parts of fulvic acid, 10~15 parts of photosynthetic bacteria.
2. the preparation method of the compound improver of water quality of aquaculture is used for, it is characterised in that following steps:
1)Photosynthetic bacteria culture medium and condition of culture:Using yeast extract, protein culture medium, basic recipe is:
CaCl20.3g, MgSO4·7H2O0.5g, yeast extract 3g, peptone 3g, distilled water 1000ml, pH:6.8, such as need to solid processed
Culture medium adds 2 parts of agar, cultivation temperature again:25 DEG C~30 DEG C optimal, intensity of illumination:2000LX~
5000LX, PH:7.5~8.5 is optimal;
2)The culture of photosynthetic bacteria:Strain is inoculated into the culture medium of bacterium of having gone out, two grades of cultures is carried out in triangular flask, daily
Rock several times, topped up with water in the reactor of 40L, be put into breather pipe, covering preservative film one, to reinstate hypochlorous acid sterilizing 24 small
When, neutralized with sodium thiosulfate, in accessing reactor with 15 parts of inoculum concentration, ventilated with pump and cultivated, with spectrophotometer with
Track measure bacterium solution density, obtain bacterium solution density it is higher when, obtain photosynthetic bacteria standby;
3)By step 2)The photosynthetic bacteria for obtaining is with bentonite, sodium humate, asarone, cupreol, fulvic acid by certain
Ratio mixes, and adds 2 times of water, stirs and evenly mixs, and 2h is adsorbed at a certain temperature, the paste of sepia is obtained, as water
Produce the compound improver of water quality of cultivation.
3. a kind of compound improver of water quality for aquaculture according to claim 2, it is characterised in that step 3)In
Described uniform temperature is 25 DEG C~30 DEG C.
4. a kind of compound improver of water quality for aquaculture according to claim 2, it is characterised in that step 3)In
Described mixing time is 30 minutes.
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| CN201611268721.6A CN106745814A (en) | 2016-12-31 | 2016-12-31 | Compound improver of water quality for aquaculture and preparation method thereof |
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| CN106745814A true CN106745814A (en) | 2017-05-31 |
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| CN201611268721.6A Pending CN106745814A (en) | 2016-12-31 | 2016-12-31 | Compound improver of water quality for aquaculture and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107683863A (en) * | 2017-07-29 | 2018-02-13 | 宿松县辉煌家庭农场 | Composite is sterilized with high-efficiency sustained-release in a kind of lobster pond |
| CN108033527A (en) * | 2017-12-12 | 2018-05-15 | 长沙拜特生物科技研究所有限公司 | Improver of water quality and preparation method thereof |
| CN108101145A (en) * | 2017-12-29 | 2018-06-01 | 武汉市天辰生物科技有限公司 | A kind of new substrate modifier used for aquiculture and preparation method thereof |
| CN108675461A (en) * | 2018-04-19 | 2018-10-19 | 金华市呗力水产养殖技术有限公司 | The preparation method of multifunctional culture water modifying agent |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1453221A (en) * | 2003-05-29 | 2003-11-05 | 中国科学院广州地球化学研究所 | Modifier for ecological environment of aquiculture pond bottom and its usage |
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| CN107683863A (en) * | 2017-07-29 | 2018-02-13 | 宿松县辉煌家庭农场 | Composite is sterilized with high-efficiency sustained-release in a kind of lobster pond |
| CN108033527A (en) * | 2017-12-12 | 2018-05-15 | 长沙拜特生物科技研究所有限公司 | Improver of water quality and preparation method thereof |
| CN108101145A (en) * | 2017-12-29 | 2018-06-01 | 武汉市天辰生物科技有限公司 | A kind of new substrate modifier used for aquiculture and preparation method thereof |
| CN108675461A (en) * | 2018-04-19 | 2018-10-19 | 金华市呗力水产养殖技术有限公司 | The preparation method of multifunctional culture water modifying agent |
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