CN106711008A - Time-of-flight mass spectrometer peak shift calibration method - Google Patents
Time-of-flight mass spectrometer peak shift calibration method Download PDFInfo
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- CN106711008A CN106711008A CN201611246739.6A CN201611246739A CN106711008A CN 106711008 A CN106711008 A CN 106711008A CN 201611246739 A CN201611246739 A CN 201611246739A CN 106711008 A CN106711008 A CN 106711008A
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- peak
- calibration method
- peak shift
- mass
- calibration
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- 238000000034 method Methods 0.000 title claims abstract description 36
- 238000001819 mass spectrum Methods 0.000 claims abstract description 24
- 239000000463 material Substances 0.000 claims abstract description 23
- 238000012360 testing method Methods 0.000 claims abstract description 9
- 238000005259 measurement Methods 0.000 claims abstract description 7
- 238000002474 experimental method Methods 0.000 claims description 16
- 239000000126 substance Substances 0.000 claims description 7
- 238000001228 spectrum Methods 0.000 claims description 5
- 230000008030 elimination Effects 0.000 claims description 3
- 238000003379 elimination reaction Methods 0.000 claims description 3
- 230000003595 spectral effect Effects 0.000 claims description 2
- 239000012925 reference material Substances 0.000 abstract description 4
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
Classifications
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/0009—Calibration of the apparatus
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/26—Mass spectrometers or separator tubes
- H01J49/34—Dynamic spectrometers
- H01J49/40—Time-of-flight spectrometers
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- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention discloses a time-of-flight mass spectrometer peak shift calibration method. Three kinds of molecular weight certified reference materials are selected according to an application range of a mass spectrometer; the three kinds of materials are subjected to 10 times of measurement through the mass spectrometer, mass spectra peak of each material is read in each test, and a shift range of the mass spectra peak is determined; the peak shift value is evaluated through a Bessel formula to serve as a calibration factor; and mass spectrum peak value of the measured material is calibrated.
Description
Technical field
The present invention relates to the technology category of the calibration of peak shift, more particularly to the peak value in a kind of time of-flight mass spectrometer
The calibration method design of drift.
Background technology
Time of-flight mass spectrometer is a particularly important analysis tool in analytical instrument, in food, chemistry and life section
Etc. field all serves huge impetus.Time of-flight mass spectrometer is using by determining the mass-to-charge ratio of material come quantitative point
The type of material is analysed, therefore obtains the accurate judgement that every kind of accurate mass spectrogram peaks of material decide result.But lot of experiments
It was found that, because ion is when passing through the Ion Extraction of time of-flight mass spectrometer and accelerating pole plate, absorption of the grid electrode to ion
Cause draw-out area voltage and accelerating region voltage fluctuation, ultimately result in flight time drift, the i.e. drift of ion spectrum peak position.Therefore
It is necessary to take measures to be calibrated for the peak shift of time of-flight mass spectrometer.
The content of the invention
The present invention discloses the calibration method of the peak shift in a kind of time of-flight mass spectrometer of invention, according to mass spectrometric suitable
3 kinds of molecular weight certified reference materials are chosen with scope, 10 measure are carried out to this 3 kinds of materials by mass spectrograph respectively, read every
The mass spectra peak of secondary substances, determines the range of drift of its mass spectra peak, and the value of peak shift is evaluated by Bessel Formula,
As calibration factor, the mass spectrogram peaks of the material to being measured are calibrated, to improve the Accurate Determining of mass spectrogram peaks.
The present invention uses following technical scheme to solve above-mentioned technical problem：
1. the calibration method of the peak shift in a kind of time of-flight mass spectrometer, it is characterised in that：It is typical by choosing
Certified reference material, asks for the stable calibration factor of experiment time of-flight mass spectrometer, and for the matter of the Instrument measuring material
During spectral peak reads.
2. the calibration method of peak shift according to claim 1, it is characterised in that：It is selected for obtaining school
The material of quasi-divisor, is that the standard that can cover whole measurement range has exhibit matter according in time of-flight mass spectrometer measurement range.
3. the calibration method of peak shift according to claim 1, wherein there is, being elimination random error, divides
Safety pin have to this 3 kinds of standards exhibit matter carry out 10 times experiment, determine its mass spectrogram, read the mass-to-charge ratio of each peak value.
4. the calibration method of peak shift according to claim 1, its feature also has：There is card for each standard
Interval where effective spectrum peak of material, reads mass spectrogram peaks；Each same substance of experiment is evaluated according to Bessel Formula
Mass spectra peak standard deviation.
5. the calibration method of peak shift according to claim 1, its feature also has：Assuming that a kind of material is i-th
Secondary mass spectrogram peaks are pi,It is 10 average peaks of experiment, then is according to the standard deviation that Bessel Formula is asked for
6. the calibration method of peak shift according to claim 1, wherein there is, Bessel Formula is obtained
Standard deviation as calibration factor, in the determination test of the mass spectrogram carried out for the instrument.
7. the calibration method of peak shift according to claim 1, wherein there is, in the calibration test for being carried out
In, 3 kinds of standards have the calibration factor striked by exhibit matter should be in given threshold values.
8. the calibration method of peak shift according to claim 1, wherein there is, having similar peak value substantially
The material of drift determines the mass spectrometric calibration factor.
9. the calibration method of peak shift according to claim 1, wherein there is, having exhibit according to 3 kinds of standards
The calibration factor that matter is asked for, during the reading of the mass spectra peaks for using it for other experiments, identified mass spectra peak should be with standard
There is the mass spectra peak of exhibit matter close.
10. the calibration method of peak shift according to claim 1, wherein there is, being calibrated using calibration factor
Test substance mass spectra peak, the drift of mass spectra peak can be eliminated.
Brief description of the drawings
Fig. 1 is the flow chart of the calibration method of the peak shift in time of-flight mass spectrometer of the invention；
Specific embodiment
Technical scheme is described in further detail below in conjunction with the accompanying drawings：
1. as shown in figure 1, a kind of calibration method of the peak shift in time of-flight mass spectrometer, it is characterised in that：Pass through
Typical certified reference material is chosen, the stable calibration factor of experiment time of-flight mass spectrometer is asked for, and survey for the instrument
During the mass spectra peak of earnest matter reads.
2. the calibration method of peak shift according to claim 1, it is characterised in that：It is selected for obtaining school
The material of quasi-divisor, is that the standard that can cover whole measurement range has exhibit matter according in time of-flight mass spectrometer measurement range.
3. the calibration method of peak shift according to claim 1, wherein there is, being elimination random error, divides
Safety pin have to this 3 kinds of standards exhibit matter carry out 10 times experiment, determine its mass spectrogram, read the mass-to-charge ratio of each peak value.
4. the calibration method of peak shift according to claim 1, its feature also has：There is card for each standard
Interval where effective spectrum peak of material, reads mass spectrogram peaks；Each same substance of experiment is evaluated according to Bessel Formula
Mass spectra peak standard deviation.
5. the calibration method of peak shift according to claim 1, its feature also has：Assuming that a kind of material is i-th
Secondary mass spectrogram peaks are pi,It is 10 average peaks of experiment, then is according to the standard deviation that Bessel Formula is asked for
6. the calibration method of peak shift according to claim 1, wherein there is, Bessel Formula is obtained
Standard deviation as calibration factor, in the determination test of the mass spectrogram carried out for the instrument.
7. the calibration method of peak shift according to claim 1, wherein there is, in the calibration test for being carried out
In, 3 kinds of standards have the calibration factor striked by exhibit matter should be in given threshold values.
8. the calibration method of peak shift according to claim 1, wherein there is, having similar peak value substantially
The material of drift determines the mass spectrometric calibration factor.
9. the calibration method of peak shift according to claim 1, wherein there is, having exhibit according to 3 kinds of standards
The calibration factor that matter is asked for, during the reading of the mass spectra peaks for using it for other experiments, identified mass spectra peak should be with standard
There is the mass spectra peak of exhibit matter close.
10. the calibration method of peak shift according to claim 1, wherein there is, being calibrated using calibration factor
Test substance mass spectra peak, the drift of mass spectra peak can be eliminated.
Claims (10)
1. the calibration method of the peak shift in a kind of time of-flight mass spectrometer, it is characterised in that：Typically there is card by choosing
Standard substance, asks for the stable calibration factor of experiment time of-flight mass spectrometer, and for the mass spectra peak of the Instrument measuring material
In reading.
2. the calibration method of peak shift according to claim 1, it is characterised in that：It is selected for obtain calibration because
The material of son, is that the standard that can cover whole measurement range has exhibit matter according in time of-flight mass spectrometer measurement range.
3. the calibration method of peak shift according to claim 1, wherein there is, being elimination random error, distinguishes pin
Having exhibit matter to this 3 kinds of standards carries out 10 experiments, determines its mass spectrogram, reads the mass-to-charge ratio of each peak value.
4. the calibration method of peak shift according to claim 1, its feature also has：There is exhibit matter for each standard
Effective spectrum peak where interval, read mass spectrogram peaks；Each matter of the same substance of experiment is evaluated according to Bessel Formula
The standard deviation of spectral peak.
5. the calibration method of peak shift according to claim 1, its feature also has：Assuming that a kind of material is in i ＆ lt matter
Spectrum peak is pi,It is 10 average peaks of experiment, then is according to the standard deviation that Bessel Formula is asked for
6. the calibration method of peak shift according to claim 1, wherein there is, the standard that Bessel Formula is obtained
Deviation as calibration factor, in the determination test of the mass spectrogram carried out for the instrument.
7. the calibration method of peak shift according to claim 1, wherein there is, in the calibration test for being carried out, 3
Kind of standard has the calibration factor striked by exhibit matter should be in given threshold values.
8. the calibration method of peak shift according to claim 1, wherein there is, having similar peak shift substantially
Material determine the mass spectrometric calibration factor.
9. the calibration method of peak shift according to claim 1, wherein there is, thering is exhibit matter to ask according to 3 kinds of standards
The calibration factor for taking, during the reading of the mass spectra peaks for using it for other experiments, identified mass spectra peak should have card with standard
The mass spectra peak of material is close.
10. the calibration method of peak shift according to claim 1, wherein there is, using treating that calibration factor is calibrated
The mass spectra peak of material is surveyed, the drift of mass spectra peak can be eliminated.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611246739.6A CN106711008A (en) | 2016-12-29 | 2016-12-29 | Time-of-flight mass spectrometer peak shift calibration method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611246739.6A CN106711008A (en) | 2016-12-29 | 2016-12-29 | Time-of-flight mass spectrometer peak shift calibration method |
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| CN106711008A true CN106711008A (en) | 2017-05-24 |
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| CN201611246739.6A Pending CN106711008A (en) | 2016-12-29 | 2016-12-29 | Time-of-flight mass spectrometer peak shift calibration method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111554559A (en) * | 2019-02-11 | 2020-08-18 | 塞莫费雪科学（不来梅）有限公司 | Mass calibration of mass spectrometers |
| CN112444556A (en) * | 2020-09-27 | 2021-03-05 | 浙江迪谱诊断技术有限公司 | Method for determining flight time nucleic acid mass spectrum parameters |
| CN112526040A (en) * | 2020-12-16 | 2021-03-19 | 杭州汇健科技有限公司 | Saliva or urine metabolism spectrum molecular weight calibrator kit and preparation method and use method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101196498A (en) * | 2006-12-06 | 2008-06-11 | 中国科学院计算技术研究所 | A Prediction Method of Mass Spectrometry Mass Measurement Error |
| CN102879511A (en) * | 2012-09-27 | 2013-01-16 | 甘肃烟草工业有限责任公司 | Method for determining volatile organic compounds in polyvinyl acetate type water-based adhesive |
-
2016
- 2016-12-29 CN CN201611246739.6A patent/CN106711008A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101196498A (en) * | 2006-12-06 | 2008-06-11 | 中国科学院计算技术研究所 | A Prediction Method of Mass Spectrometry Mass Measurement Error |
| CN102879511A (en) * | 2012-09-27 | 2013-01-16 | 甘肃烟草工业有限责任公司 | Method for determining volatile organic compounds in polyvinyl acetate type water-based adhesive |
Non-Patent Citations (1)
| Title |
|---|
| 中国计量科学研究院: "《飞行时间质谱仪校准规范》", 《中华人民共和国国家计量技术规范》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111554559A (en) * | 2019-02-11 | 2020-08-18 | 塞莫费雪科学（不来梅）有限公司 | Mass calibration of mass spectrometers |
| CN111554559B (en) * | 2019-02-11 | 2023-05-26 | 塞莫费雪科学（不来梅）有限公司 | Mass Calibration for Mass Spectrometers |
| CN112444556A (en) * | 2020-09-27 | 2021-03-05 | 浙江迪谱诊断技术有限公司 | Method for determining flight time nucleic acid mass spectrum parameters |
| CN112444556B (en) * | 2020-09-27 | 2021-12-03 | 浙江迪谱诊断技术有限公司 | Method for determining flight time nucleic acid mass spectrum parameters |
| CN112526040A (en) * | 2020-12-16 | 2021-03-19 | 杭州汇健科技有限公司 | Saliva or urine metabolism spectrum molecular weight calibrator kit and preparation method and use method thereof |
| CN112526040B (en) * | 2020-12-16 | 2022-07-26 | 杭州汇健科技有限公司 | Saliva or urine metabolism spectrum molecular weight calibrator kit and preparation method and use method thereof |
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