CN106644989B - Absorbance detection system - Google Patents
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- CN106644989B CN106644989B CN201710057381.0A CN201710057381A CN106644989B CN 106644989 B CN106644989 B CN 106644989B CN 201710057381 A CN201710057381 A CN 201710057381A CN 106644989 B CN106644989 B CN 106644989B
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- 238000002835 absorbance Methods 0.000 title claims abstract description 74
- 238000001514 detection method Methods 0.000 title claims abstract description 73
- 238000003384 imaging method Methods 0.000 claims abstract description 41
- 238000009434 installation Methods 0.000 claims abstract description 37
- 239000003086 colorant Substances 0.000 claims abstract description 5
- 239000000463 material Substances 0.000 claims description 13
- 238000005192 partition Methods 0.000 claims description 10
- 238000004891 communication Methods 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 2
- 238000012360 testing method Methods 0.000 abstract description 11
- 238000005259 measurement Methods 0.000 abstract description 3
- 239000013307 optical fiber Substances 0.000 description 29
- 238000012986 modification Methods 0.000 description 17
- 230000004048 modification Effects 0.000 description 17
- 238000010586 diagram Methods 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 4
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 4
- 239000010931 gold Substances 0.000 description 4
- 229910052737 gold Inorganic materials 0.000 description 4
- 238000005286 illumination Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000011481 absorbance measurement Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/255—Details, e.g. use of specially adapted sources, lighting or optical systems
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/062—LED's
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/063—Illuminating optical parts
- G01N2201/0631—Homogeneising elements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/063—Illuminating optical parts
- G01N2201/0636—Reflectors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/063—Illuminating optical parts
- G01N2201/0638—Refractive parts
- G01N2201/0639—Sphere lens
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/08—Optical fibres; light guides
- G01N2201/0813—Arrangement of collimator tubes, glass or empty
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses an absorbance detection system which comprises a supporting mechanism, a light source mechanism, an imaging mechanism and a processor. The supporting mechanism is provided with at least two light outlets and at least one light inlet; the device comprises at least two mounting cavities and at least one reference cavity, wherein the mounting cavities and the reference cavity are arranged on the supporting mechanism, one ends of the mounting cavities and the reference cavity are communicated with the light inlet, and the other ends of the mounting cavities and the reference cavity are respectively in one-to-one correspondence and are communicated with the light outlet; the installation cavity is used for inserting the light-transmitting container; the light source mechanism is used for providing light sources with required colors for the installation cavity and the reference cavity through the light inlet; the imaging mechanism is used for shooting images of the solution to be detected and the reference cavity after being irradiated by the light source through the light outlet; and the processor obtains an absorbance value of the solution to be detected in the installation cavity according to the image information acquired by the imaging mechanism. The absorbance value of the measured solution can be obtained by only measuring once as a blank test without placing any solution in the reference cavity, thereby realizing rapid measurement of the absorbance of the solution.
Description
Technical Field
The invention relates to the technical fields of food safety detection, environmental safety detection and biochemical detection, in particular to a detection system for absorbance.
Background
Absorbance is a physical quantity for measuring the absorption degree of light, and refers to the ratio of the incident light intensity before light passes through a solution or a certain substance to the transmitted light intensity after the light passes through the solution or the substance, and is mainly influenced by the wavelength of light, the thickness of the solution, the concentration of solute in the solution and the like.
At present, absorbance detection mainly has two technical approaches, namely, a spectrophotometer measurement principle is adopted, white light source is adopted for illumination, and high-spectrum resolution absorbance measurement in a wide spectrum range can be realized through prism or grating light splitting, so that the application range is wide, the precision is high, the price is high, the volume is large, the operation is relatively complex, the special technical personnel are required to use the device, and the device is not suitable for the rapid detection requirement under the field condition; and secondly, the multi-color selectable optical filter is adopted to measure the absorbance of multiple bands, the price of the instrument is relatively low, but the problem of poor portability still exists.
Chinese patent document CN104502293A discloses a food safety rapid detection device based on a mobile phone platform. The mobile phone with the CCD camera comprises a mobile phone with the CCD camera, a light gathering piece arranged above the CCD camera of the mobile phone through a fixing piece, a detection main body arranged on the fixing piece, a cuvette arranged inside the detection main body, a first LED light source (emitting red light) and a second LED light source (emitting green light), wherein the cuvette is arranged between the light gathering piece and the two LED light sources.
The detection device with the structure is characterized in that when the absorbance of a solution is detected, a first LED light source works, detection is carried out twice, and states of no nano gold reaction solution and no nano gold reaction solution are respectively detected in a cuvette to obtain the first absorbance of the solution corresponding to the first light source; and then the second LED light source works, the first LED light source does not work, and the detection is carried out twice, the states of no nano gold reaction solution and no nano gold reaction solution are respectively detected in the cuvette, the second absorbance of the solution corresponding to the second light source is obtained, the ratio of the first absorbance to the second absorbance is obtained, and then the concentration of the solution to be detected is calculated. That is, the above detection device needs to be tested four times, and the red light detection is performed twice and the green light detection is performed twice, so that the concentration of the solution to be detected can be calculated, resulting in the defects of long whole detection time period and low detection efficiency.
Disclosure of Invention
Therefore, the technical problem to be solved by the invention is to overcome the problems of long detection period and low detection efficiency of the absorbance detection device in the prior art.
To this end, the invention provides a system for detecting absorbance comprising
The support mechanism is provided with at least two light outlets and at least one light inlet; the light source comprises at least one mounting cavity and at least one reference cavity, wherein the mounting cavity is arranged on the supporting mechanism, the reference cavity is separated from the mounting cavity, one end of each of the mounting cavity and the reference cavity is communicated with the light inlet, and the other end of each of the mounting cavity and the reference cavity is respectively in one-to-one correspondence and communication with the light outlets; the mounting cavity is internally used for inserting a light-transmitting container filled with a solution to be detected or a light-transmitting material;
the light source mechanism is used for providing light sources with required colors for the installation cavity and the reference cavity through the light inlet;
the imaging mechanism is used for respectively shooting images of the solution to be detected or the light-transmitting material irradiated by the light source and the reference cavity through the light outlet;
and the processor is used for obtaining the absorbance value of the solution or the light-transmitting material to be detected in the installation cavity according to the image information acquired by the imaging mechanism.
Preferably, the absorbance detection system comprises a base and a supporting table formed on the base; the mounting cavity and the reference cavity are both formed on the supporting table, and the light inlet and the light outlet are formed on the supporting table.
Further preferably, in the absorbance detection system, the supporting table is formed with an inner cavity along a horizontal direction, and the installation cavity is vertically formed on the top of the supporting table, extends downwards and is communicated with the inner cavity; the reference cavity is arranged on the supporting table and is positioned in the inner cavity;
the one end opening of inner chamber forms the light inlet, and the shaping has sealed baffle on the other end opening, set up on the baffle with installation cavity with reference cavity respectively the first light outlet and the second light outlet of one-to-one.
More preferably, in the absorbance detection system, the supporting mechanism further includes a first baffle formed in the inner cavity and disposed parallel to the partition plate, the first baffle divides the inner cavity into a first cavity facing the light outlet and a second cavity facing the light inlet, and the mounting cavity is located in the first cavity;
and two second baffles arranged in the first cavity in parallel, wherein one ends of the two second baffles are formed on the first baffle, and the other ends of the two second baffles are formed on the inner wall surface of the partition plate where the second light outlet is formed, so as to enclose the reference cavity;
and the first baffle is provided with a first through hole and a second through hole which are respectively in one-to-one correspondence with the first light outlet and the second light outlet.
Preferably, in the absorbance detection system, the mounting cavity is a blind hole.
Preferably, in the above absorbance detection system, the light source mechanism includes a light emitter, and a light pipe connecting a light emitting surface of the light emitter with the light inlet.
Further preferably, in the absorbance detection system, the light source mechanism further includes a second light homogenizing plate vertically disposed in the supporting mechanism and located between the mounting cavity or the reference cavity and the light inlet.
Further preferably, in the absorbance detection system, a horizontal groove suitable for embedding the light pipe is formed in the bottom of the base, and an inclined perforation is formed in the supporting table, one end of the perforation is communicated with the horizontal groove, the other end of the perforation is communicated with the inner cavity and faces the light inlet, so that the light outlet end of the light pipe is installed in the perforation;
the light source mechanism also comprises a first reflecting mirror obliquely arranged on the light inlet and used for changing the direction of a light source irradiated by the light pipe so that the light source irradiates the mounting cavity and the reference cavity in the horizontal direction; and/or
The light source mechanism further includes a first light homogenizing sheet horizontally covering the perforated top surface.
Preferably, in the above absorbance detection system, the imaging mechanism includes an imaging lens, and a second reflecting mirror installed between the imaging lens and the light outlet, so that the light source vertically irradiates on the imaging lens after passing through the second reflecting mirror.
Further preferably, in the absorbance detection system, the imaging mechanism further includes a focusing lens located between the second reflecting mirror and the lens, and the lens is aligned with the center of the lens and separated by a preset gap.
More preferably, in the above absorbance detection system, the illuminant is a flash lamp of a mobile phone, the imaging lens is a photographing lens of the mobile phone, and the flash lamp and the photographing lens are both located at the back of the mobile phone;
the mobile phone also comprises a first mounting seat for mounting the light inlet end of the light pipe and the lens on the mobile phone, so that the flash lamp is opposite to the light inlet end of the light pipe, and the lens is opposite to the lens.
Preferably, in the absorbance detection system, the first mounting seat is provided with a third through hole and a fourth through hole, the third through hole is wound around the flash lamp, and the light inlet end of the light pipe is fixed in the third through hole;
the fourth through hole is wound around the lens, the lens is installed in the fourth through hole, and a required gap is reserved between one end of the lens, which faces the lens, and the lens.
Further preferably, in the absorbance detection system, two ends of the fourth through hole are respectively provided with a step surface;
the step surface facing to one side of the lens is used for being overlapped by the end surface of the lens, the step surface facing away from one side of the lens is used for installing the lens, and the interval between the two step surfaces is the gap.
Preferably, in the absorbance detection system, two ends of the light pipe are respectively sleeved with a fixed sleeve; and/or
The longitudinal section of the first mounting seat is L-shaped, the third through hole is formed in the vertical part of the L-shape, and the fourth through hole Kong Kaishe is formed in the horizontal part of the L-shape; and/or
The longitudinal section of the supporting table is trapezoidal, the long side of the trapezoid is formed on the base, the two side edges are respectively a vertical side and an inclined side, the inclined side is provided with the light inlet, and the vertical side is provided with the light outlet; and/or
The number of the installation cavities is at least two, the number of the reference cavities is at least one, and the reference cavities are arranged between two adjacent installation cavities.
The technical scheme of the invention has the following advantages:
1. the invention provides an absorbance detection system which comprises a supporting mechanism, a light source mechanism, an imaging mechanism and a processor. The support mechanism is provided with at least two light outlets and at least one light inlet; the light source comprises at least one mounting cavity and at least one reference cavity, wherein the mounting cavity is arranged on the supporting mechanism, the reference cavity is separated from the mounting cavity, one end of each of the mounting cavity and the reference cavity is communicated with the light inlet, and the other end of each of the mounting cavity and the reference cavity is respectively in one-to-one correspondence and communication with the light outlets; the mounting cavity is internally used for inserting a light-transmitting container filled with a solution to be detected or a light-transmitting material; the light source mechanism is used for providing light sources with required colors for the installation cavity and the reference cavity through the light inlet; the imaging mechanism is used for respectively shooting images of the solution to be detected or the light-transmitting material irradiated by the light source and the reference cavity through the light outlet; and the processor obtains the absorbance value of the solution or the light-transmitting material to be detected in the installation cavity according to the image information acquired by the imaging mechanism.
According to the absorbance detection system with the structure, at least one mounting cavity and at least one reference cavity are arranged on the supporting mechanism, when the absorbance of a solution is required to be detected, the solution to be detected is only required to be placed into the light-transmitting container, the light-transmitting container is mounted in the mounting cavity, the light source mechanism is adopted to irradiate the solution in the light-transmitting container in the mounting cavity through the light inlet, the reference cavity is irradiated, any solution is not placed in the reference cavity as a blank test, and the imaging mechanism is used for respectively shooting images of the solution to be detected or the light-transmitting material irradiated by the light source through the light outlet and the reference cavity; the processor can calculate the absorbance value of the measured solution by comparing the R, G, B chromaticity value or the gray value in the image of the measured solution with the image corresponding to the reference cavity. The absorbance value of the measured solution can be obtained by the detection system by only measuring once, and the absorbance value of the measured solution can be obtained without adopting a four-time measurement mode in a comparison file, so that the absorbance of the solution is rapidly measured.
2. The invention provides a detection system for absorbance, wherein the support mechanism further comprises a first baffle plate which is formed in the inner cavity and is arranged in parallel with the baffle plate, the first baffle plate divides the inner cavity into a first cavity facing to one side of the light outlet and a second cavity facing to one side of the light inlet, and the installation cavity is positioned in the first cavity; and two second baffles arranged in the first cavity in parallel, wherein one ends of the two second baffles are formed on the first baffle, and the other ends of the two second baffles are formed on the inner wall surface of the partition plate where the second light outlet is formed, so as to enclose the reference cavity; and the first baffle is provided with a first through hole and a second through hole which are respectively in one-to-one correspondence with the first light outlet and the second light outlet.
The absorbance detection system with the structure is characterized in that the first baffle and the second baffle formed in the inner cavity of the support body are used for enclosing the reference cavity, the first baffle is used for isolating the reference cavity from the installation cavity, when light emitted by the luminous body enters the second cavity through the light inlet, the solution to be detected in the installation cavity and the reference cavity enclosed by the second baffle are respectively irradiated through the first through hole and the second through hole, and then the first light outlet and the second light outlet are irradiated on the imaging mechanism, so that images of the illuminated solution to be detected and the reference cavity are obtained, and the processor is used for processing image data to obtain absorbance values of the solution to be detected.
3. The light source mechanism comprises a luminous body and a light pipe connecting the light emitting surface of the luminous body with the light inlet. Further preferably, the light source mechanism further comprises a second light homogenizing plate vertically arranged in the supporting mechanism and located between the mounting cavity or the reference cavity and the light inlet. More preferably, the light source mechanism further comprises a first light homogenizing sheet horizontally covering the perforated top surface. The light guide tube is arranged, the position and the direction of a light source emitted by the luminous body are conveniently changed, the first light homogenizing sheet is used for performing primary light distribution on light output by the light guide tube, so that the light is uniformly irradiated on the first reflecting mirror, the second light homogenizing sheet performs secondary light distribution on the light output by the optical fiber, the secondary light distribution realizes uniform back illumination of a solution to be detected, and the different positions of the solution to be detected can be irradiated by the same light, so that the detection accuracy is improved.
4. The imaging mechanism comprises an imaging lens, and a second reflecting mirror arranged between the imaging lens and the light outlet, so that the light source vertically irradiates on the imaging lens after passing through the second reflecting mirror. Further, the imaging mechanism further includes a focusing lens located between the second mirror and the lens, the lens being aligned with the center of the lens and separated by a preset gap. The second reflecting mirror is arranged, so that the light source irradiates the lens in the vertical direction, and the lens can acquire the images of the solution to be detected and the reference cavity more accurately; the focusing lens is convenient to adjust the focal length, so that the image shot by the lens is clearer.
5. According to the absorbance detection system provided by the invention, the illuminant is the flash lamp of the mobile phone, the imaging lens is the shooting lens of the mobile phone, and the flash lamp and the shooting lens are positioned on the back surface of the mobile phone; the mobile phone also comprises a first mounting seat for mounting the light inlet end of the light pipe and the lens on the mobile phone, so that the flash lamp is opposite to the light inlet end of the light pipe, and the lens is opposite to the lens.
The absorbance detection system with the structure adopts the flash lamp of the mobile phone as a light source, the camera of the mobile phone as an imaging mechanism, the acquisition of images is facilitated, and the acquired images are analyzed by the automatic processor of the mobile phone, so that the absorbance value of the solution to be detected is obtained, and the carrying and the rapid detection of the detection system are facilitated.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the structure of a first embodiment of the absorbance detection system provided in example 1 of the invention (dashed lines and arrows indicate the directions of light beams);
fig. 2 is a schematic structural diagram of a supporting mechanism (including a first reflecting mirror, a first light homogenizing sheet and a second light homogenizing sheet) in the absorbance detection system provided in embodiment 1 of the present invention;
FIG. 3 is a schematic longitudinal cross-sectional view of the support mechanism of FIG. 2;
FIG. 4 is a schematic view of the support mechanism of FIG. 2 (with the first mirror, the first light homogenizing sheet, and the second light homogenizing sheet removed);
FIG. 5 is a schematic view (rear view) of the support mechanism of FIG. 2;
FIG. 6 is a schematic cross-sectional view of the support mechanism of FIG. 2;
FIG. 7 is a schematic diagram of the structure of the first mounting seat in the absorbance detection system according to embodiment 1 of the invention;
FIG. 8 is a schematic longitudinal cross-sectional view of the first mount of FIG. 7;
FIG. 9 is a schematic diagram showing the assembly of the second mount and the second mirror in the absorbance detection system according to embodiment 1 of the invention;
FIG. 10 is a schematic view of the second mounting base of FIG. 9;
FIG. 11 is a schematic diagram of the structure of a second embodiment of the absorbance detection system provided in example 1 of the invention;
FIG. 12 is a schematic diagram of the structure of a third embodiment of the absorbance detection system provided in example 1 of the invention;
reference numerals illustrate: 1-a supporting mechanism; 11-a light inlet; 121-a first light outlet; 122-a second light outlet; 13-a base; 131-horizontal grooves; 132-perforating; 133-a first fixing hole; 14-a support table; 2-mounting a cavity; 3-reference cavity; 4-a first baffle; 41-a first through hole; 42-a second through hole; 5-a second baffle; 61-luminophores; 62-a light pipe; 63—a first mirror; 64-a second light homogenizing plate; 65-a first light homogenizing sheet; 71-lens; 72-lens; 73-a second mirror; 8-a mobile phone; 9-a first mounting base; 91-a third through hole; 911-second fixing holes; 94-fourth through holes; 95-fixing the sleeve; 10-test tube; 15-a second mounting seat.
Detailed Description
The following description of the embodiments of the present invention will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the invention are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
In addition, the technical features of the different embodiments of the present invention described below may be combined with each other as long as they do not collide with each other.
Example 1
As shown in fig. 1, the present embodiment provides a system for detecting absorbance, which includes a support mechanism 1, a light source mechanism, an imaging mechanism, and a processor.
As shown in fig. 2 to 5, the supporting mechanism 1 includes a base 13 and a supporting table 14 formed on the base 13, the supporting table 14 is formed with an inner cavity along a horizontal direction, an opening at one end of the inner cavity forms a light inlet 11, a sealing partition is formed on an opening at the other end, two first light outlets 121 and a second light outlet 122 are formed on the partition, and the second light outlet 122 is located between the two first light outlets 121 and is spaced from the first light outlet 121. Two installation cavities 2 extending downwards vertically are arranged on the top of the supporting table 14, and the two installation cavities 2 are communicated with the inner cavity; a first baffle 4 and two second baffles 5 are also formed in the inner cavity of the supporting table 14, and the first baffle 4 is arranged in parallel with the baffle; along the horizontal direction, the first baffle 4 divides the inner cavity into a first cavity facing the light outlet side and a second cavity facing the light inlet 11 side, and the installation cavity 2 is positioned in the first cavity; two second baffles 5 are arranged in parallel in the first cavity, one end of each second baffle is formed on the first baffle 4, the other end of each second baffle is formed on the inner wall surface of the position where the second light outlet 122 is formed in the partition plate, so that a reference cavity 3 is formed, and the reference cavity 3 is located between the two installation cavities 2.
As shown in fig. 5, the first baffle 4 is provided with a first through hole 41 and a second through hole 42 corresponding to the first light outlet 121 and the second light outlet 122 one by one. Two first through holes 41 correspond to two mounting cavities 2, one second through hole 42 corresponds to one reference cavity 3, and the first through holes 41 and the second through holes 42 are arranged so that the mounting cavities 2 and the reference cavity 3 communicate with the light inlet 11.
Preferably, the mounting cavity 2 is a mounting blind hole formed on the supporting table 14, the bottom of the blind hole is located below the inner cavity, the blind hole is communicated with the second cavity and the first light outlet 121 at the position of the first through hole 41 to form a detection window, a light-transmitting container for the solution to be detected, such as a test tube 10, is mounted in the blind hole through the top of the supporting table 14, and the solution to be detected in the test tube leaks out of the first cavity at the first through hole 41, so that light irradiates the test tube through the first through hole 41, passes through the solution to be detected and then is emitted through the first light outlet 121.
The longitudinal section of the supporting table 14 is trapezoidal, the long sides of the trapezoid are formed on the base 13, the two sides are respectively a vertical side and an inclined side, the inclined side is provided with the light inlet 11, and the vertical side is provided with two first light outlets 121 and one second light outlet 122. The top of the support table 14 has an upwardly extending extension at the location where the mounting cavity 2 is open. The positions of the base 13 extending out of the periphery of the supporting table 14 are provided with mounting holes, for example, four corners are respectively provided with a mounting hole, and the base 13 is mounted on the workbench surface by a fastener penetrating through the mounting holes, for example, the fastener is a bolt assembly or a screw.
The light source mechanism is used for providing light sources of required colors, such as white light sources, green light sources, red light sources and the like, into the mounting cavity 2 and the reference cavity 3 through the light inlet 11, and as shown in fig. 1, the light source mechanism comprises a light emitting body 61, a light pipe 62, a first reflecting mirror 63, a first light homogenizing sheet 65 and a second light homogenizing sheet 64.
The illuminant 61 is preferably an LED lamp, and the illuminant is white light, so as to absorb light with different wavelengths required by the solution to be detected. The light pipe 62 is preferably an optical fiber, such as a single-core optical fiber, the diameter of the optical fiber bundle or the diameter of the single-core optical fiber is preferably not less than 0.2mm, the light-entering end of the optical fiber is directed toward the LED lamp, and the distance between the end face of the light-entering end of the optical fiber and the light-emitting surface of the LED lamp is preferably less than 5mm. The first reflecting mirror 63 is disposed on the light inlet 11 of the supporting table 14, and is disposed in an inclined manner, and as shown in fig. 3, the second light homogenizing plate 64 is vertically parallel to the first baffle 4 and is mounted on a surface of the first baffle 4 facing the light inlet 11 side, so as to shield the first through hole 41 and the second through hole 42; the first light homogenizing sheet 65 is horizontally disposed in the second cavity and covers the top surface of the perforation. The first light homogenizing sheet 65 is configured so that light emitted by the optical fiber is first homogenized to form uniform light and irradiates on the first reflecting mirror 63, the uniform light is reflected by the first reflecting mirror 63 and then can be irradiated on the second light homogenizing plate 64 along the horizontal direction (i.e. perpendicularly irradiated on the second light homogenizing plate 64), and the second light homogenizing plate 64 is utilized to perform the second light homogenizing treatment on the light output by the optical fiber, so that uniform back illumination of the solution to be detected is realized, and different positions of the solution to be detected can be irradiated by the same light, thereby improving the detection accuracy. Preferably, the second light homogenizing plate 64 is spaced less than 5mm, or some other distance, from the surface of the cuvette 10. Preferably, the thickness of the first light homogenizing sheet 65 is 2mm, or less than 2mm, such as 1mm, 1.5mm, 1.8mm, etc.
As shown in fig. 1, the imaging mechanism includes a second mirror 73, a focusing lens 72, and a lens 71. The lens 71 and the illuminant 61 respectively adopt a shooting lens 71 and a flash lamp of a camera on the mobile phone 8, and adopt a processor of the mobile phone 8 to directly process the acquired image, so as to directly obtain the absorbance value of the solution to be detected. The focusing lens 72 is disposed between the second reflecting mirror 73 and the lens 71, and is used for adjusting the focal length so that the image shot by the lens 71 is clearer; preferably, the focal length of the focusing lens 72 is 50-100mm, most preferably 70mm.
The absorbance detection system further includes a first mount 9 for mounting the light entrance end of the optical fiber and the focusing lens 72 on the back of the mobile phone 8 so that the flash is opposite to the light entrance end of the light pipe 62 and the center of the lens 71 is opposite to the center of the lens 72. As shown in fig. 7 and 8, the first mounting seat 9 has an L-shaped longitudinal cross-section, a fourth through hole 94 is formed in a horizontal portion of the L-shape, and a third through hole 91 is formed in a vertical portion of the L-shape. Wherein, the third through hole 91 is wound around the flash lamp, and the light inlet end of the optical fiber is fixed in the third through hole 91; the two ends of the fourth through hole 94 are respectively provided with a step surface, the step surface facing to one side of the lens 71 is used for embedding and abutting against the end surface of the lens 71, the step surface facing to one side of the lens 71 is used for installing the lens 72, the distance between the two step surfaces is a required gap reserved between the lens 71 and the lens 72, for example, 3mm or less than 3mm, the required gap is adjusted according to actual use requirements, but after the two step surfaces are processed, the gap between the lens 72 and the lens 71 is a fixed value, so that when images are acquired for different solutions or light-transmitting materials, the imaging mechanism is kept at the same level, and the detection accuracy of the imaging mechanism is improved. In addition, the arrangement of the two step surfaces is convenient for positioning the installation position of the fourth through hole 94 when the first installation seat 9 is fixed on the back surface of the mobile phone 8; the other annular step surface is used for mounting the focusing lens 72 and also facilitates positioning and mounting of the focusing lens 72.
As shown in fig. 3 and 6, the two ends of the optical fiber are respectively sleeved with a fixing sleeve 95, the bottom of the base 13 is provided with a horizontal groove 131 suitable for embedding the light pipe 62, and an inclined perforation 132 arranged in the supporting table 14, one end of the perforation 132 is communicated with the horizontal groove 131, the other end of the perforation 132 is communicated with the inner cavity and faces the light inlet 11, and the inclined perforation 132 is matched with the inclined angle of the first reflecting mirror 63 only by the two, so that the light outlet end of the optical fiber irradiates on the first reflecting mirror 63, and the light source reflected by the first reflecting mirror 63 irradiates in the installation cavity 2 and the reference cavity 3 along the horizontal direction.
The both ends of the optical fiber are respectively disposed in the third through hole 91 and the inclined through hole 132 by the fixing sleeve 95. Two second fixing holes 911 are formed in the side wall of the third through hole 91, fasteners are respectively arranged in the two second fixing holes 911, the outer surfaces of the fasteners are connected in the third through hole 91 in a threaded mode, one end of each fastener extending into the second fixing hole 911 is abutted against the fixing sleeve 95, and therefore the light inlet end of the optical fiber is fixed in the third through hole 91; similarly, the base 13 and the supporting table 14 are provided with inclined first fixing holes 133, the first fixing holes 133 are communicated with the through holes 132, the fastening members are in threaded connection in the first fixing holes 133, and one ends of the fastening members extending into the first fixing holes 133 are pressed against the fixing sleeve 95, so that the light emitting ends of the optical fibers are fixed in the through holes 132 and face the first reflecting mirror 63. The design of the horizontal groove 131 ensures that when the base 13 is placed horizontally, the optical fiber does not protrude out of the base 13, the placement stability of the base 13 is not affected, and the optical fiber is installed and positioned.
In addition, the above-mentioned mobile phone 8 is horizontally disposed, the flash lamp and the lens 71 at the back of the mobile phone 8 face down, the second reflector 73 is disposed on the second mounting seat 15, as shown in fig. 9 and 10, the second mounting seat 15 has an inclined surface, the second reflector 73 is adhered on the inclined surface through glue, the inclination angle of the second reflector 73 is preferably 45 °, so as to realize 90 turning of the imaging beam, or other inclination angles, only the light irradiated from the second light outlet 122 and the first light outlet 121 is reflected by the second reflector 73, the light source irradiates on the lens 72 along the vertical direction, the solution to be detected after the light irradiation is imaged, and the specific inclination angle is determined according to the actual use situation.
In addition, the distance between the flash lamp and the imaging lens 71 is preferably greater than 8mm, and the distance between the light inlet end of the optical fiber and the flash lamp is preferably less than 5mm; the spacing between the center of the second mirror 73 and the center of the lens 72 is 20-40mm, for example 20mm, 35mm, 38mm, 40mm, and so on. Optimally, the sum of the distance from the second mirror 73 to the lens 72 and the distance from the first mirror 63 to the center of the cuvette 10 is the focal length of the lens 72.
In the absorbance detection system in the above embodiment, before detecting the absorbance of the solution to be detected, the solution to be detected is first loaded into the test tube 10, the test tube 10 is inserted into the blind hole downwards through the top of the supporting table 14, and the solution in the test tube leaks out at the detection window of the first through hole 41; reference chamber 3 was used as a blank control; the mobile phone 8 is operated to enable the flash lamp to emit a light source, the light beam is transmitted by the optical fiber, the light emitting end of the optical fiber obliquely arranged in the perforation 132 irradiates on the first light homogenizing sheet 65 in an oblique direction, is subjected to primary light distribution, then irradiates on the first reflecting mirror 63, the first reflecting mirror 63 irradiates the light source irradiated by the optical fiber on the second light homogenizing plate 64 in a horizontal direction, the second light homogenizing plate 64 irradiates light for the second time, the solution to be detected forms uniform backlight illumination, the light source irradiates in the test tube 10 in the blind hole and the reference cavity through the first through hole 41 and the second through hole 42 respectively, the light beam irradiates on the second reflecting mirror 73 through the first light emitting opening 121 and the second light emitting opening 122, and then irradiates on the lens 72 vertically after being reflected by the second reflecting mirror 73, an image of the solution to be detected is formed on the mobile phone 8 through the lens 71, and a processor on the mobile phone 8 obtains the absorbance value of the solution to be detected by comparing the gray value or the R, G, B value of the center area of each solution to be detected with the reference cavity 3. The absorbance values of the two solutions to be detected with different concentrations can be detected only by one time of testing, or the absorbance values of the solutions to be detected are not needed to be detected, and the absorbance values of the solutions to be detected are not needed to be measured for four times like the prior art, so that the detection efficiency of the detection system is improved. Meanwhile, the flash lamp and the lens 71 of the mobile phone 8 are adopted as a light source and an image collector in the detection process, so that the whole detection system is simple in structure and convenient to carry, and the light source is not required to be independently arranged in a way of being separated from the mobile phone 8.
In addition, the arrangement of the first light homogenizing sheet 65 and the second light homogenizing sheet 64 makes the light irradiated into the solution to be detected and the reference cavity 3 more uniform, improving the detection accuracy thereof. The optical fiber irradiates on the first reflector at a certain inclination angle, and the arrangement of the first reflector 63 can increase the distance between the light emitting end of the optical fiber and the light homogenizing plate, improve the uniformity of irradiation on the second light homogenizing plate 64, and simultaneously is beneficial to reducing the space occupied by the light source mechanism.
As an alternative to the above-described embodiments, there are the following:
as an alternative embodiment, the above-mentioned mounting cavities 2 may also be one, three, four, five, etc., and correspondingly, the reference cavities 3 may be one, two, three, four, etc., and when the mounting cavities 2 are plural, one reference cavity 3 is disposed between two adjacent mounting cavities 2 in order to facilitate distinguishing the plural mounting cavities 2.
As a modification of the support stand 14, the longitudinal cross-sectional shape of the support stand 14 may be other shapes, for example, L-shape or rectangular shape, and it is only necessary that the support stand 14 has a horizontal inner cavity, one end of which is opened with the light inlet 11, and the other end of which is opened with the light outlet, and the light outlets are in one-to-one correspondence with the mounting cavity 2 and the reference cavity 3 and are spaced from each other.
As a modification of the first mount 9, the longitudinal cross-sectional shape of the first mount 9 may be rectangular, or may be any other shape, and the third through hole 91 and the fourth through hole 94 may be formed. The fourth through hole 94 may have only one stepped surface for positioning and mounting the lens 72, and the lens 71 on the back side of the cell phone 8 is surrounded by the other end of the fourth through hole 94. As a modification, only the third through hole 91 and the fourth through hole 94 are required to be formed on the first mounting seat 9, and the third through hole 91 is wound around the flash lamp; the fourth through hole 94 is wound around the lens 71, and a required gap is reserved between the lens 71 and one end of the lens 72 facing the lens 71, and the size of the gap is determined according to actual use conditions.
As a further modification, the first mount 9 may be omitted, and the light-entering end of the optical fiber and the lens 72 may be supported by other supports near the back of the mobile phone 8, so that the flash is opposite to the light-entering end of the light pipe 62 and the lens 71 is opposite to the lens 72. As a further modification, the mobile phone 8 may be replaced by other electronic devices, such as a tablet computer, which only needs to have a flash or a light source, and the photographing lens 71, or other lighting devices may be used to provide a light source with a desired color; correspondingly, the photographing lens 71 may be a separate photographing device, and only a light source is needed to cooperate with photographing to detect the absorbance value of the solution to be detected. The light emitter 61 and the lens 71 may be located on the same electronic device, or may be separately provided.
As a modification of the imaging mechanism, the imaging mechanism may also be configured to take an image directly using the lens 71 of the smartphone 8 with high pixels without providing the above-described focusing lens 72; in a further modification, the imaging mechanism may be configured such that the lens 71 is directly disposed opposite to the light outlet without providing the second reflecting mirror 73. As a variant, the imaging mechanism may also be other existing devices for acquiring images.
As a modification, the horizontal groove 131 may be replaced by an extension hole formed in the base, and one end of the extension hole is located on the sidewall of the base 13, and the other end of the extension hole is connected to the through hole 132.
As a modification of the light source mechanism, as shown in fig. 11, the first light-homogenizing sheet 65 may not be provided in the light source mechanism, and the light emitted from the light-emitting end of the optical fiber may be directly irradiated onto the first reflecting mirror 63, reflected by the first reflecting mirror 63, and then irradiated onto the second light-homogenizing sheet 64, where the light is subjected to only one-time light-homogenizing treatment by the second light-homogenizing sheet; alternatively, as shown in fig. 12, the light source mechanism may be configured such that the first reflecting mirror 63 and the first light homogenizing sheet 65 are not provided, and the light emitted from the light emitting end of the optical fiber is directly irradiated onto the second light homogenizing sheet 64; alternatively, as a modification, the first reflecting mirror 63, the first light homogenizing sheet 65, and the second light homogenizing sheet 64 may not be provided, and the light source may be directly irradiated into the second cavity through the light inlet 11. Correspondingly, the horizontal grooves 131 and the through holes 132 are not required to be respectively formed on the base 13 and the supporting table 14. As a modification of the light pipe 62, the light pipe 62 may be other conventional light transmission structures in addition to an optical fiber. As a further modification, the light emitting surface of the light emitting body 61 may be directly faced to the light inlet 11 without providing the optical fiber. As a modification of the light emitter 61, the light emitter 61 may be an LED lamp, or other white light lamp, or a color lamp.
As a variant, the mounting cavity 2 may also be of other shape, such as a recess, or a vertically extending through-hole structure. Further, the support mechanism 1 may be provided with the required mounting cavity 2 and reference cavity 3 directly on the support table 14 without providing the first baffle 4 and the second baffle 5.
As a modification, the support stand 14 may have other structures, and only the mounting cavity 2 and the reference cavity 3 are formed on the support stand 14, and the support stand 14 may be provided with the light inlet 11 and the light outlet.
Further, according to a further modification, the supporting mechanism 1 may also have other structures, for example, the supporting mechanism 1 includes a frame-shaped mounting frame, a baffle is disposed on the mounting frame, the baffle and the mounting frame enclose a closed cavity, one end of the closed cavity is a light inlet 11, the other end is a light outlet, a plurality of partitions are disposed in the mounting cavity 2, a mounting cavity 2 or a reference cavity 3 is formed between two adjacent partitions, and the reference cavity 3 and the mounting cavity 2 are disposed at intervals.
As a variant, the support mechanism 1 described above need only have at least two light outlets and at least one light inlet 11; at least one installation cavity 2 and at least one reference cavity 3 which are separated from the installation cavity 2 are arranged on the supporting mechanism 1, one end of each of the installation cavity 2 and the reference cavity 3 is communicated with the light inlet 11, and the other end of each of the installation cavity 2 and the reference cavity 3 is respectively in one-to-one correspondence with and communicated with the light outlet; the installation cavity 2 is used for inserting a light-transmitting container filled with a solution to be detected or a light-transmitting material.
The detection system described above preferably detects the absorbance of a solution, and may detect the absorbance of a light-transmitting material, such as plastic or glass.
It is apparent that the above examples are given by way of illustration only and are not limiting of the embodiments. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. While still being apparent from variations or modifications that may be made by those skilled in the art are within the scope of the invention.
Claims (10)
1. An absorbance detection system comprising
The support mechanism (1) is provided with at least two light outlets and at least one light inlet (11); at least one installation cavity (2) arranged on the supporting mechanism (1) and at least one reference cavity (3) separated from the installation cavity (2), wherein one end of each of the installation cavity (2) and the reference cavity (3) is communicated with the light inlet (11), and the other end of each of the installation cavity and the reference cavity is respectively in one-to-one correspondence and communication with the light outlet; the installation cavity (2) is internally provided with a light-transmitting container filled with a solution to be detected or a light-transmitting material;
the light source mechanism is used for providing light sources with required colors for the installation cavity (2) and the reference cavity (3) through the light inlet (11);
the imaging mechanism is used for respectively shooting images of the solution to be detected or the light-transmitting material irradiated by the light source and the reference cavity (3) through the light outlet;
the processor is used for obtaining an absorbance value of the solution or the light-transmitting material to be detected in the installation cavity (2) according to the image information acquired by the imaging mechanism;
the supporting mechanism (1) comprises a base (13) and a supporting table (14) formed on the base (13); the mounting cavity (2) and the reference cavity (3) are both formed on the supporting table (14), and the supporting table (14) is provided with the light inlet (11) and the light outlet;
the light source mechanism comprises a luminous body (61) and a light pipe (62) for connecting the light emitting surface of the luminous body (61) with the light inlet (11);
the light source mechanism further comprises a second light homogenizing plate (64) vertically arranged in the supporting mechanism (1) and positioned between the mounting cavity (2) or the reference cavity (3) and the light inlet (11);
the imaging mechanism comprises an imaging lens (71), and a second reflecting mirror (73) arranged between the imaging lens (71) and the light outlet, so that the light source vertically irradiates on the imaging lens (71) after passing through the second reflecting mirror (73).
2. The absorbance detection system of claim 1 wherein: the supporting table (14) is provided with an inner cavity along the horizontal direction, and the installation cavity (2) is vertically arranged on the top of the supporting table (14), extends downwards and is communicated with the inner cavity; the reference cavity (3) is arranged on the supporting table (14) and is positioned in the inner cavity;
one end opening of the inner cavity forms the light inlet (11), the other end opening is provided with a sealing baffle plate, and the baffle plate is provided with a first light outlet (121) and a second light outlet (122) which are respectively in one-to-one correspondence with the installation cavity (2) and the reference cavity (3).
3. The absorbance detection system of claim 2 wherein: the supporting mechanism (1) further comprises a first baffle (4) which is formed in the inner cavity and is arranged in parallel with the partition board, the first baffle (4) divides the inner cavity into a first cavity towards one side of the light outlet and a second cavity towards one side of the light inlet (11), and the mounting cavity (2) is positioned in the first cavity;
and two second baffles (5) arranged in parallel in the first cavity, one ends of the two second baffles (5) are formed on the first baffle (4), and the other ends of the two second baffles are formed on the inner wall surface of the partition plate where the second light outlet (122) is formed, so as to enclose the reference cavity (3);
the first baffle (4) is provided with a first through hole (41) and a second through hole (42) which are respectively in one-to-one correspondence with the first light outlet (121) and the second light outlet (122).
4. A system for detecting absorbance according to claim 2 or 3 wherein: the mounting cavity (2) is a blind hole.
5. The absorbance detection system of claim 3 wherein: a horizontal groove (131) suitable for embedding the light pipe (62) is formed in the bottom of the base (13), and an inclined perforation (132) is formed in the supporting table (14), one end of the perforation (132) is communicated with the horizontal groove (131), the other end of the perforation is communicated with the inner cavity and faces the light inlet (11), and the light emitting end of the light pipe (62) is arranged in the perforation (132);
the light source mechanism further comprises a first reflecting mirror (63) obliquely arranged on the light inlet (11) and used for changing the direction of a light source irradiated by the light guide pipe (62) so as to irradiate the light source into the mounting cavity (2) and the reference cavity (3) along the horizontal direction; and/or
The light source mechanism further includes a first light homogenizing sheet (65) horizontally covering the top surface of the perforation (132).
6. The absorbance detection system of claim 1 wherein: the imaging mechanism further includes a focusing lens (72) located between the second reflecting mirror (73) and the lens (71), the lens (72) being aligned with the center of the lens (71) and separated by a preset gap.
7. The absorbance detection system of claim 6 wherein: the luminous body (61) is a flash lamp of the mobile phone (8), the imaging lens (71) is a shooting lens (71) of the mobile phone (8), and the flash lamp and the shooting lens (71) are both positioned on the back of the mobile phone (8);
the mobile phone also comprises a first mounting seat (9) for mounting the light inlet end of the light pipe (62) and the lens (72) on the mobile phone (8), so that the flash lamp is opposite to the light inlet end of the light pipe (62), and the lens (71) is opposite to the lens (72).
8. The absorbance detection system of claim 7 wherein: the first mounting seat (9) is provided with a third through hole (91) and a fourth through hole (94), the third through hole (91) is wound around the flash lamp, and the light inlet end of the light pipe (62) is fixed in the third through hole (91);
the fourth through hole (94) is wound around the lens (71), the lens (72) is installed in the fourth through hole (94), and a required gap is reserved between one end, facing the lens (71), of the lens and the lens (71).
9. The absorbance detection system of claim 8 wherein: both ends of the fourth through hole (94) are provided with step surfaces:
the step surface facing to one side of the lens (71) is used for being overlapped by the end surface of the lens (71), the step surface facing away from one side of the lens (71) is used for mounting the lens (72), and the interval between the two step surfaces is the gap.
10. The absorbance detection system according to claim 8 or 9 wherein: two ends of the light pipe (62) are respectively sleeved with a fixed sleeve (95); and/or
The longitudinal section of the first mounting seat (9) is L-shaped, the third through hole (91) is formed in the vertical part of the L-shape, and the fourth through hole (94) is formed in the horizontal part of the L-shape; and/or
The longitudinal section of the supporting table (14) is trapezoidal, the long side of the trapezoid is formed on the base (13), the two side edges are respectively a vertical side and an inclined side, the inclined side is provided with the light inlet (11), and the light outlet is formed in the vertical side; and/or
The number of the installation cavities (2) is at least two, the number of the reference cavities (3) is at least one, and the reference cavities (3) are arranged between two adjacent installation cavities (2).
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