CN106511506A - Traditional Chinese medicine composition for treating diabetes, preparation method and application thereof - Google Patents

Abstract

The invention belongs to the technical field of medicines and especially relates to a traditional Chinese medicine composition for treating diabetes, a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 45-60 parts of dogwood, 20-35 parts of white paeony root and 20-35 parts of astragalus membranaceus. In the traditional Chinese medicine composition, dogwood has the effects of tonifying liver and kidney, astringing and inducing astringency; the white paeony root has the effects of stabilizing liver yang, astringing yin and reducing sweat; the astragalus membranaceus has the effects of tonifying qi, lifting yang and consolidating exterior for arresting sweating; the materials are compounded on the basis of a synergic principle, can mutually effect and have the effects of reducing blood glucose and quenching thirst, promoting blood circulation to remove blood stasis, and replenishing qi to invigorate the spleen; and the traditional Chinese medicine composition can achieve the purposes of reducing blood glucose and treating diabetes in various aspects and has a better effect. A test result proves that the effect of the traditional Chinese medicine composition provided by the invention in reducing blood glucose of a diabetic mouse is better than the effect of the traditional Chinese medicine composition composed of acarbose and similar components.

Description

A kind of traditional Chinese medicine composition for treating diabetes and its preparation method and application

technical field

The invention belongs to the technical field of medicines, and in particular relates to a traditional Chinese medicine composition for treating diabetes and its preparation method and application.

Background technique

Diabetes mellitus is a metabolic disease characterized by elevated blood glucose levels caused by absolute or relative insufficiency of insulin secretion. Diabetes is usually diagnosed with blood glucose levels. When the fasting blood glucose level is ≥7.0mmol/L or has the characteristic symptoms of diabetes and the blood glucose level is ≥11.1mmol/L at any time, it is diagnosed as having diabetes. Its clinical symptoms are "three excesses and one deficiency", that is, polyuria, polydipsia, polyphagia, and weight loss. The long-term high blood sugar environment of the body will lead to lesions of many tissues and organs such as eyes, kidneys, heart, etc., which are collectively referred to as diabetic complications. The pathogenesis and treatment mechanisms of diabetes and its complications are complex and diverse.

With the rapid development of the economy and the improvement of people's living standards, the incidence of diabetes is increasing year by year. At present, diabetes has become the most important chronic non-infectious disease that seriously endangers human health after tumors and cardiovascular diseases. The global investment in diabetes treatment is increasing year by year, and there are more and more reports at home and abroad on the research and development of hypoglycemic drugs and the research on the mechanism of hypoglycemia.

At present, the commonly used hypoglycemic drugs are mainly western medicines, mainly insulin and its analogs, sulfonylurea secretagogues, metformin, α-glucosidase inhibitors, thiazolidinedione derivatives sensitizers , phenylanilic acid derivatives secretagogues, GLP-1 receptor agonists and DDP-4 enzyme inhibitors eight categories. Although these medicines can effectively lower blood sugar, the price of western medicine is relatively expensive, and adverse reactions are prone to occur in long-term use. Therefore, it is very necessary to develop a Chinese patent medicine with good hypoglycemic effect.

Contents of the invention

In view of this, the object of the present invention is to provide a traditional Chinese medicine composition for treating diabetes and its preparation method and application. The traditional Chinese medicine composition provided by the present invention has a good hypoglycemic effect.

The invention provides a traditional Chinese medicine composition, which is made of raw materials comprising the following components in parts by weight:

Cornus officinalis 45-60 parts;

Radix Paeoniae Alba 20～35 parts;

Astragalus 20-35 parts.

Preferably, the traditional Chinese medicine composition is composed of the following components in parts by weight: 46-60 parts of Cornus officinalis, 21-34 parts of Radix Paeoniae Alba and 21-34 parts of Radix Astragali.

Preferably, the traditional Chinese medicine composition is composed of the following components in parts by weight: 47-58 parts of Cornus officinalis, 22-34 parts of Radix Paeoniae Alba and 22-34 parts of Radix Astragali.

The present invention provides a kind of preparation method of Chinese medicine composition described in above-mentioned technical scheme, comprises the following steps:

After the raw material is extracted with alcohol solvent, the extract is concentrated to obtain the traditional Chinese medicine composition.

Preferably, the alcoholic solvent includes ethanol.

Preferably, the volume ratio of the raw material to the alcohol solvent is 1:(8-10).

Preferably, the extraction method is reflux extraction.

Preferably, the relative density of the traditional Chinese medicine composition at 25°C is 1.15-1.35.

The invention provides a traditional Chinese medicine preparation, which is made from the traditional Chinese medicine composition described in the above technical scheme or the traditional Chinese medicine composition prepared by the method described in the above technical scheme, and pharmaceutically acceptable auxiliary materials.

Application of the traditional Chinese medicine composition described in the above technical scheme or the traditional Chinese medicine composition prepared by the method described in the above technical scheme in the preparation of medicines for treating diabetes.

Compared with the prior art, the invention provides a traditional Chinese medicine composition for treating diabetes and its preparation method and application. The traditional Chinese medicine composition is prepared from raw materials including the following components in parts by weight: 45-60 parts of cornus officinalis; 20-35 parts of white peony; and 20-35 parts of astragalus. In the present invention, Cornus officinalis nourishes liver and kidney, astringes and solidifies astringency; Radix Paeoniae Alba suppresses liver yang, restrains yin and absorbs sweat; Astragalus nourishes qi and promotes yang, strengthens surface and stops sweating; the above-mentioned several substances are compounded and interacted with each other according to the orders of the monarchs and ministers. It has the effects of lowering blood sugar and quenching thirst, promoting blood circulation and removing blood stasis, replenishing qi and invigorating the spleen, and achieves the purpose of lowering blood sugar and treating diabetes from multiple angles, and has good effects. Experimental results show that the hypoglycemic effect of the traditional Chinese medicine composition provided by the invention on diabetic mice is better than that of acarbose and traditional Chinese medicine compositions with similar compositions.

detailed description

The following clearly and completely describes the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

The invention provides a traditional Chinese medicine composition, which is made of raw materials comprising the following components in parts by weight:

Cornus officinalis 45-60 parts;

Radix Paeoniae Alba 20～35 parts;

Astragalus 20-35 parts.

Among them, the present invention has no special limitation on the sources of all raw materials, which can be commercially available.

The raw materials of the traditional Chinese medicine composition provided by the invention include Cornus officinalis, Radix Paeoniae Alba and Radix Astragali. Among them, the dried and mature pulp of Cornus officinalis. Sour, astringent, lukewarm, returns to the liver and kidney meridians; it can nourish the liver and kidney, astringent essence and detoxification. Its pharmacological effects are mainly manifested in the aspects of lowering blood sugar, inhibiting bacteria, and inhibiting platelet aggregation. In the present invention, the content of the Cornus officinalis in raw materials is 45-60 parts by weight, preferably 46-60 parts by weight, more preferably 47-58 parts by weight, specifically 47.5 parts by weight, 48 parts by weight, 50 parts by weight parts, 51 parts by weight or 55.5 parts by weight.

In the present invention, Radix Paeoniae Alba is the dry root of Radix Paeoniae Alba. Bitter, sour, slightly cold in nature; returns to the liver and spleen meridian; has the effects of nourishing blood, regulating menstruation, and calming liver yang. In the present invention, the content of the Radix Paeoniae Alba in raw materials is 20 to 35 parts by weight, preferably 21 to 34 parts by weight, more preferably 22 to 34 parts by weight, specifically 22 parts by weight, 23 parts by weight, 23.5 parts by weight parts by weight, 25 parts by weight or 24.5 parts by weight.

In the present invention, Astragalus is the dried root of the leguminous plant Astragalus mongolica or Astragalus membranaceus. Sweet in taste, slightly warm; returns lung, spleen meridian. It has the effects of invigorating qi and promoting yang, solidifying the surface and antiperspirant, diuretic and swelling, promoting body fluid and nourishing blood. In the present invention, the content of the astragalus in the raw material is 20-35 parts by weight, preferably 21-34 parts by weight, more preferably 22-34 parts by weight, specifically 22 parts by weight, 23 parts by weight, 23.5 parts by weight parts, 25 parts by weight or 24.5 parts by weight.

In some embodiments provided by the present invention, the traditional Chinese medicine composition is made of the following components by weight: 47.5 parts by weight of Cornus officinalis, 23.5 parts by weight of Radix Paeoniae Alba, and 23.5 parts by weight of Radix Astragali.

In some embodiments provided by the present invention, the traditional Chinese medicine composition is made of the following components by weight: 48 parts by weight of Cornus officinalis, 25 parts by weight of Radix Paeoniae Alba, and 25 parts by weight of Radix Astragali.

In some embodiments provided by the present invention, the traditional Chinese medicine composition is made of the following components by weight: 50 parts by weight of Cornus officinalis, 24.5 parts by weight of Radix Paeoniae Alba, and 24.5 parts by weight of Radix Astragali.

In some embodiments provided by the present invention, the traditional Chinese medicine composition is made of the following components by weight: 51 parts by weight of Cornus officinalis, 23 parts by weight of Radix Paeoniae Alba, and 23 parts by weight of Radix Astragali.

In some other embodiments provided by the present invention, the traditional Chinese medicine composition is made of the following components by weight: 55.5 parts by weight of Cornus officinalis, 22 parts by weight of Radix Paeoniae Alba, and 22 parts by weight of Radix Astragali.

In the present invention, the active ingredients of Cornus officinalis, Radix Paeoniae Alba, and Radix Astragalus are formulated according to the order of the emperor and ministers. Cornus officinalis is the monarch drug, and Radix Paeoniae Alba and Astragalus are the ministerial drugs. Among them, Cornus officinalis nourishes the liver and kidney, astringes and solidifies astringency; Radix Paeoniae Alba suppresses liver-yang and restrains yin. Sweating; Radix Astragali invigorates qi and raises yang, strengthens the surface and stops sweating. The above-mentioned several substances are compounded and interacted with each other according to the order of the emperor and ministers, and have the effects of lowering blood sugar and quenching thirst, promoting blood circulation and removing blood stasis, replenishing qi and invigorating the spleen, and achieving the goals of lowering blood sugar and treating diabetes from multiple angles, and have good effects. Experimental results show that the hypoglycemic effect of the traditional Chinese medicine composition provided by the invention on diabetic mice is better than that of acarbose and traditional Chinese medicine compositions with similar compositions.

The present invention provides a kind of preparation method of Chinese medicine composition described in above-mentioned technical scheme, comprises the following steps:

After the raw material is extracted with alcohol solvent, the extract is concentrated to obtain the traditional Chinese medicine composition.

In the preparation method provided by the invention, the raw materials are firstly extracted with an alcoholic solvent. Wherein, the raw materials include Cornus officinalis, Radix Paeoniae Alba and Radix Astragali; the alcohol solvent includes but not limited to ethanol. In one embodiment provided by the present invention, the alcohol solvent is an aqueous ethanol solution with a volume concentration of 65-75%. In the present invention, the volume ratio of the raw material to the alcohol solvent is preferably 1: (8-10); the extraction method is preferably reflux extraction; the extraction time is preferably 1.5-2 hours. In the present invention, the raw material is preferably extracted multiple times with an alcoholic solvent, and the number of extractions is preferably 2 to 3 times. After the extraction is completed, an extract is obtained.

After obtaining the extract, the extract is concentrated. Wherein, the method of concentration is preferably concentration under reduced pressure. The present invention has no special limitation on the concentration time, and the extract can be concentrated to the required concentration. After the concentration is completed, the traditional Chinese medicine composition is obtained. In an embodiment provided by the present invention, the relative density of the traditional Chinese medicine composition is 1.15-1.35. In the present invention, the relative density is the ratio of the density of the traditional Chinese medicine composition to the density of water at 25°C under the same pressure conditions.

The traditional Chinese medicine composition with good hypoglycemic effect can be prepared by adopting the method provided by the invention. Experimental results show that the hypoglycemic effect of the traditional Chinese medicine composition prepared by the method provided by the invention on diabetic mice is better than that of acarbose and traditional Chinese medicine compositions with similar compositions.

The invention provides a traditional Chinese medicine preparation, which is made of the above-mentioned traditional Chinese medicine composition and pharmaceutically acceptable auxiliary materials, and the auxiliary materials are preferably soluble starch, dextrin, microcrystalline cellulose, mannitol, steviol glycoside, and magnesium stearate One or more of them; the traditional Chinese medicine preparation can be granules, capsules or tablets.

The present invention also provides an application of the above-mentioned traditional Chinese medicine composition in the preparation of medicaments for treating diabetes.

In order to further illustrate the present invention, a Chinese medicine composition provided by the present invention, its preparation method and Chinese medicine preparation are described in detail below in conjunction with the examples.

The reagents used in the following examples are all commercially available.

Example 1

Mix 47.5 parts by weight of Cornus officinalis, 23.5 parts by weight of Radix Paeoniae Alba and 23.5 parts by weight of Radix Astragali, add 8 to 10 times the volume of 70% ethanol, reflux extraction for 2 to 3 times, each time for 1.5 to 2 hours, mix the extracted filtrate, reduce Concentrate under pressure to make a medicinal solution with a concentration of 0.165g crude drug/mL, and the relative density of the medicinal solution is 1.15-1.35 (25°C).

Comparative example 1

Mix 70 parts by weight of Cornus officinalis, 15 parts by weight of Radix Paeoniae Alba and 15 parts by weight of Astragalus membranaceus, add 8 to 10 times the volume of 70% ethanol, reflux and extract for 2 to 3 times, each time for 1.5 to 2 hours, mix the extracted filtrate, reduce Concentrate under pressure to make a medicinal solution with a concentration of 0.165g crude drug/mL, and the relative density of the medicinal solution is 1.15-1.35 (25°C).

The Chinese medicine composition obtained in Comparative Example 1 is compared with the Chinese medicine composition obtained in Example 1 for treating diabetes, and the specific experimental process is as follows:

Pharmacodynamic investigation experiment:

Experimental animals: male Kunming mice, weighing 20±2g, provided by the Experimental Animal Center of Jilin University.

Experimental drug: the medicinal solution obtained in Example 1; the medicinal solution obtained in Comparative Example 1;

Positive control: acarbose;

Experimental grouping and modeling: Diabetic model, 50 mice were randomly divided into normal group 10, model group 40, fasted for 12 hours, intraperitoneal injection of STZ 50mg/kg (once a day, 5 consecutive days), in After 7 days of modeling, cut the tail and take blood to measure the blood sugar value (fasting overnight before the test), if it is greater than 16mmol/L, the modeling is successful and a diabetic model is formed;

Experimental administration: the modeling group was randomly divided into a model group, a Chinese medicine composition administration group and a positive control group. The Chinese medicinal liquid obtained in Example 1, the Chinese medicinal liquid obtained in Comparative Example 1, and the positive drug acarbose were administered by intragastric administration, once a day, for 7 consecutive days, and fasted for 12 hours before administration on the 7th day. The blood glucose level of the mice was measured 1 h after administration.

As shown in Table 1, the mice in each group were administered once a day. After 7 days, they were fasted for 12 hours before the administration on the 7th day, and the blood glucose of the mice was measured 1 hour after the administration. The results are shown in Table 2:

Table 1 Experimental drug administration

Table 2 Blood sugar and blood sugar reduction percentage after administration

The experimental results show that the traditional Chinese medicine extract prepared in Example 1 has a better effect, and has a certain effect on lowering blood sugar in diabetic mice. The traditional Chinese medicine extract prepared in Comparative Example 1 has a weak therapeutic effect on diabetic mice, and the degree of hypoglycemia is small.

Example 2

Mix 48 parts by weight of Cornus officinalis, 25 parts by weight of Radix Paeoniae Alba and 25 parts by weight of Astragalus membranaceus, add 8 to 10 times the volume of 70% ethanol, reflux and extract for 2 to 3 times, each time for 1.5 to 2 hours, mix the extracted filtrate, reduce Concentrate under pressure until the relative density is 1.15-1.35 (25°C) to obtain the traditional Chinese medicine composition.

According to the above experimental method, the pharmacodynamic investigation experiment was carried out. Except that Comparative Example 1 was not set, the experimental administration was consistent with Table 1. On the 7th day, before the administration, fasting was performed for 12 hours, and the blood glucose of the mice was measured 1 hour after the administration. , the results are shown in Table 3:

Table 3 Blood sugar and blood sugar reduction percentage after administration

It can be seen from the data in the table that the traditional Chinese medicine composition prepared in Example 2 has a better effect of lowering blood sugar on diabetic mice.

Example 3

Mix 50 parts by weight of Cornus officinalis, 24.5 parts by weight of Radix Paeoniae Alba and 24.5 parts by weight of Astragalus membranaceus, add 8 to 10 times the volume of 70% ethanol, reflux extraction for 2 to 3 times, each time for 1.5 to 2 hours, mix the extracted filtrate, reduce Concentrate under pressure until the relative density is 1.15-1.35 (25°C) to obtain the traditional Chinese medicine composition. Then add dextrin (with the weight ratio of the traditional Chinese medicine composition being 160%, that is, 100g of the traditional Chinese medicine composition adds 160g of dextrin), steviol glycoside (with the weight ratio of the dextrin is 1%) and other auxiliary materials are mixed evenly, and the soft material is passed through 14 mesh Sieve and granulate, dry at 60-65°C for 2 hours, pass through a 14-mesh sieve for granulation, and obtain traditional Chinese medicine preparation granules.

According to the above experimental method, the pharmacodynamic investigation experiment was carried out. Except that Comparative Example 1 was not set, the experimental administration was consistent with Table 1. On the 7th day, before the administration, fasting was performed for 12 hours, and the blood glucose of the mice was measured 1 hour after the administration. , the results are shown in Table 4:

Table 4 Blood sugar and blood sugar reduction percentage after administration

It can be seen from the data in the table that the traditional Chinese medicine granules prepared in Example 3 have a better effect of lowering blood sugar on diabetic mice.

Example 4

Mix 51 parts by weight of Cornus officinalis, 23 parts by weight of Radix Paeoniae Alba and 23 parts by weight of Astragalus membranaceus, add 8 to 10 times the volume of 70% ethanol, reflux and extract for 2 to 3 times, each time for 1.5 to 2 hours, mix the extracted filtrate, reduce Concentrate under pressure until the relative density is 1.15-1.35 (25°C) to obtain the traditional Chinese medicine composition. Then add dextrin (85% by weight with the traditional Chinese medicine composition), mix evenly with soluble starch (75% by weight with the traditional Chinese medicine composition), make granules, dry and granulate, fill capsules, and obtain Chinese medicine preparation capsules.

According to the above experimental method, the pharmacodynamic investigation experiment was carried out. Except that Comparative Example 1 was not set, the experimental administration was consistent with Table 1. On the 7th day, before the administration, fasting was performed for 12 hours, and the blood glucose of the mice was measured 1 hour after the administration. , the results are shown in Table 5:

Table 5 Blood sugar and blood sugar reduction percentage after administration

It can be seen from the data in the table that the traditional Chinese medicine capsule prepared in Example 4 has a better effect of lowering blood sugar on diabetic mice.

Example 5

Mix 55.5 parts by weight of Cornus officinalis, 22 parts by weight of Radix Paeoniae Alba and 22 parts by weight of Astragalus membranaceus, add 8 to 10 times the volume of 70% ethanol, reflux and extract for 2 to 3 times, each time for 1.5 to 2 hours, mix the extracted filtrate, reduce Concentrate under pressure until the relative density is 1.15-1.35 (25°C) to obtain the traditional Chinese medicine composition. Then add dextrin (with the weight ratio of Chinese medicine composition is 137%), microcrystalline cellulose (with the weight ratio of Chinese medicine composition is 14%), mannitol (with the weight ratio of Chinese medicine composition is 8%), steviol glycoside (with the weight ratio of Chinese medicine composition is 8%), The traditional Chinese medicine composition is 1% by weight) and other auxiliary materials are mixed evenly, granulated, dried and granulated, magnesium stearate (1% by weight of the traditional Chinese medicine composition) is added and compressed into tablets to obtain Chinese medicine preparation tablets.

According to the above experimental method, the pharmacodynamic investigation experiment was carried out. Except that Comparative Example 1 was not set, the experimental administration was consistent with Table 1. On the 7th day, before the administration, fasting was performed for 12 hours, and the blood glucose of the mice was measured 1 hour after the administration. , the results are shown in Table 6:

Table 6 Blood sugar and blood sugar reduction percentage after administration

It can be seen from the data in the table that the traditional Chinese medicine tablet prepared in Example 5 has a better effect of lowering blood sugar on diabetic mice.

The above is only a preferred embodiment of the present invention, it should be pointed out that, for those of ordinary skill in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, and these improvements and modifications can also be made. It should be regarded as the protection scope of the present invention.

Claims (10)

1. a kind of Chinese medicine composition, is made up of the raw material including following parts by weight of component：

45～60 parts of Fructus Corni；

20～35 parts of the Radix Paeoniae Alba；

20～35 parts of the Radix Astragali.

2. Chinese medicine composition according to claim 1, it is characterised in that be made up of the component including following weight portion：Mountain 21～34 parts of 46～60 parts of the Fructus Evodiae, 21～34 parts of the Radix Paeoniae Alba and the Radix Astragali.

3. Chinese medicine composition according to claim 2, it is characterised in that be made up of the component including following weight portion：Mountain 22～34 parts of 47～58 parts of the Fructus Evodiae, 22～34 parts of the Radix Paeoniae Alba and the Radix Astragali.

4. a kind of preparation method of Chinese medicine composition described in claim 1, comprises the following steps：

After being extracted to raw material with alcohols solvent, extracting solution is concentrated, obtain Chinese medicine composition.

5. preparation method according to claim 4, it is characterised in that the alcohols solvent includes ethanol.

6. preparation method according to claim 4, it is characterised in that the raw material is 1 with the volume ratio of alcohols solvent：(8 ～10).

7. preparation method according to claim 4, it is characterised in that the mode of the extraction is reflux, extract,.

8. the preparation method according to any one of claim 4～7, it is characterised in that the Chinese medicine composition is at 25 DEG C Relative density be 1.15～1.35.

9. a kind of Chinese medicine preparation, the Chinese medicine composition or claim 4～8 by described in claims 1 to 3 any one are any one Chinese medicine composition prepared by methods described, and pharmaceutically acceptable adjuvant makes.

10. it is prepared by Chinese medicine composition or claim 4～8 any one methods described described in claims 1 to 3 any one Chinese medicine composition prepare treatment diabetes medicament in application.