CN1064811C - Application method of direct seading rice - Google Patents
Application method of direct seading rice Download PDFInfo
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- CN1064811C CN1064811C CN971098158A CN97109815A CN1064811C CN 1064811 C CN1064811 C CN 1064811C CN 971098158 A CN971098158 A CN 971098158A CN 97109815 A CN97109815 A CN 97109815A CN 1064811 C CN1064811 C CN 1064811C
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- Prior art keywords
- rice
- herbicide
- direct
- gene
- variety
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 50
- 235000009566 rice Nutrition 0.000 title claims abstract description 50
- 238000000034 method Methods 0.000 title claims abstract description 24
- 240000007594 Oryza sativa Species 0.000 title description 2
- 241000209094 Oryza Species 0.000 claims abstract description 50
- 239000004009 herbicide Substances 0.000 claims abstract description 48
- 241000196324 Embryophyta Species 0.000 claims abstract description 26
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 24
- 230000002363 herbicidal effect Effects 0.000 claims abstract description 23
- 238000010899 nucleation Methods 0.000 claims abstract description 10
- ZBMRKNMTMPPMMK-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid;azane Chemical group [NH4+].CP(O)(=O)CCC(N)C([O-])=O ZBMRKNMTMPPMMK-UHFFFAOYSA-N 0.000 claims description 8
- 230000009261 transgenic effect Effects 0.000 claims description 6
- 101150103518 bar gene Proteins 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 3
- 230000001404 mediated effect Effects 0.000 claims description 2
- 238000011426 transformation method Methods 0.000 claims 2
- 241000589158 Agrobacterium Species 0.000 claims 1
- 238000005507 spraying Methods 0.000 abstract description 2
- 230000003247 decreasing effect Effects 0.000 abstract 1
- YZHUMGUJCQRKBT-UHFFFAOYSA-M sodium chlorate Chemical compound [Na+].[O-]Cl(=O)=O YZHUMGUJCQRKBT-UHFFFAOYSA-M 0.000 description 12
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N Phosphinothricin Natural products CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 9
- IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 description 9
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 6
- 238000009825 accumulation Methods 0.000 description 3
- 229910021529 ammonia Inorganic materials 0.000 description 3
- 238000009331 sowing Methods 0.000 description 3
- 125000003277 amino group Chemical group 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 125000003338 L-glutaminyl group Chemical class O=C([*])[C@](N([H])[H])([H])C([H])([H])C([H])([H])C(=O)N([H])[H] 0.000 description 1
- 241000187391 Streptomyces hygroscopicus Species 0.000 description 1
- 241001655322 Streptomycetales Species 0.000 description 1
- GINJFDRNADDBIN-FXQIFTODSA-N bilanafos Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCP(C)(O)=O GINJFDRNADDBIN-FXQIFTODSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000009355 double cropping Methods 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000009333 weeding Methods 0.000 description 1
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Abstract
The present invention discloses an application method of direct seading rice, which comprises the following steps: herbicide resistance genes are transferred into a direct seeding rice variety to make the direct seeding rice variety obtain the herbicide resistance characteristics; the direct seeding rice variety of the transferred genes is sown, and a herbicide is sprayed to remove weeds of a rice field and plants left by front rice. With the method of the present invention, the weeds of a rice field and the plants left by the front rice can be eliminated by spraying the herbicide, and the method is safe for the direct seeding rice variety. Agricultural cultivation with time saving and labor saving is realized, labor intensity can be reduced, and agricultural cost can also be decreased.
Description
The present invention relates to a kind of application method of direct seading rice.
The many in the world paddy rice plantations of rice direct-seeding technology country is by widespread usage, and China is faced with rapid economic development, and it is imperative to widely popularize the rice direct-seeding technology.Yet China's south rice planting area mostly is two season plantations, and the plant of eliminating the farmland running to weeds of direct sowing rice field and other rice varieties that preceding stubble rice field stays is to realize China's two difficult problems that season is live or the single cropping late rice is live in double cropping rice area.Whole nation accumulative total direct seading rice cultivated area is 1,000 ten thousand mu during " eight or five ", expectation the enforcement period of the ninth five-year plan will be developed into 3,000 ten thousand mu, if can solve the farmland running to weeds problem that runs into when direct seading rice is produced, national directly seeding rice area will be developed by leaps and bounds, and obtains good economic and social benefit.
Anti-herbicide gene (bar) separates from moist streptomycete (Strep Tomyceshygroscopicus) and obtains, its phosphino-thricin (PPT) transacetylase (PAT) of encoding.This gene outcome (PAT) can make the acetylization of PPT terminal amino group.Bialaphos in the weed killer herbicide and PPT mainly contain effective constituent, for a kind of L-glutamic acid analog, are the potent inhibitors of glutamy synzyme.It is generally acknowledged that the PPT that generates in the plant corpus can suppress the glutamy synzyme and lead the accumulation of system ammonia, plant is poisoned to death because of the ammonia accumulation.Because the bar gene PAT that can encode, make the free amine group acetylization on the PPT in the Basta weed killer herbicide and remove the toxicity of PPT.This gene has been used for the Study on Transformation of various plants, is one of screening marker gene that produces the high-efficient transgenic plant.
Development of biology is that the successful Application of direct seading rice has been brought hope.
The object of the present invention is to provide a kind of direct seading rice application method that can overcome above-mentioned weak point of the prior art.
The object of the present invention is achieved like this:
A kind of application method of direct seading rice may further comprise the steps: anti-herbicide gene is changed over to have can carry out in the live rice varieties, make this kind obtain the antiweed characteristic; Genetically modified direct seading rice kind sowed down sprays the corresponding weed killer herbicide of described anti-herbicide gene, with kill weeds in paddy field and before the plant that stays of stubble paddy rice.
The anti-herbicide gene that adopts is the bar gene in the present invention, but it will be apparent to those skilled in the art that other anti-herbicide gene of separating clone can be applicable among the present invention equally.
In the present invention, the rice varieties that is used for the transgenosis transformation have can carry out live be, the capital draws 119, in hundred No. 1, elegant water 11 and spring river 03, but, design of the present invention can be applicable to other equally can carry out the direct seading rice kind, even can be used for the plant of other kind.
In the present invention, because employing is the bar gene, used weed killer herbicide is basta.It will be understood by those skilled in the art that then used weed killer herbicide also can be done corresponding adjustment if what adopt is other anti-herbicide gene.
The method that anti-herbicide gene is changed in the direct seading rice kind is a method known in those skilled in the art, comprises the agriculture bacillus mediated conversion method, electric shocking method, cotransformation method and the particle gun blast technique that are widely adopted at present.The particle gun blast technique is adopted in special recommendation of the present invention.
Advantage of the present invention is:
Can make the direct seading rice kind obtain anti-weeding gene after using the inventive method.Therefore, behind herbicide spraying, both can eliminate weeds in paddy field, the plant that the stubble paddy rice stays before can eliminating again, and it is fool proof for the direct seading rice kind, really reach the light-duty agricultural cultivation of time and labour saving, can reduce labor intensity, can reduce agricultural cost again.
Fig. 1 is paddy rice transfer-gen plant and the non-transgenic plant photo to the Herbicid resistant result of the test.Wherein, A represents that the non-transgenic plant is killed by weed killer herbicide, and B represents that the transfer-gen plant growth is normal.
With way of example the inventive method is done more detailed description below.
In June, 1996, by experimental scheme, as the direct seading rice kind, system is sowed by strain for seed (band anti-herbicide gene bar) with transgenosis T3, each strain system sowing 40 strain in the land for growing field crops, Hangzhou.Compare (not containing anti-herbicide gene bar) every 20 row kind of a non-transgenic rice varieties Zhenshan 97a,, spray the 0.25%Basta weed killer herbicide in full heading time, week back observation, the result is as shown in Figure 1.All growths are normal in the picture, and it is colony for transgenosis T3 for strain that blade keeps green person, and because of importing has anti-herbicide gene bar, this gene produces the toxicity that can decompose PPT in the weed killer herbicide, can not cause and be poisoned to death, and weed killer herbicide Basta is shown as strong resistance; Otherwise non-transgenic common rice kind plant lacks anti-herbicide gene, and the PPT composition makes ammonia accumulation causing poisoning in these paddy rice bodies among the weed killer herbicide Basta, and blade is withered and yellow to cause death, and is all killed by weed killer herbicide Basta in a week.Obviously, after weed killer herbicide Basta sprays, when killing weeds in paddy field, also can kill batch other rice varieties plant that stays (lacking the bar gene) before the rice field, and fool proof to the rice plant that contains anti-herbicide gene bar.
According to The above results, after anti-herbicide gene imports the direct seading rice kind, other rice varieties plant that stubble stays before weeds in paddy field and the removing rice field always just is readily solved, both alleviated peasant's labour intensity, guarantee direct sowing rice field and seed purity again, all the time can not cause to mix, demonstrate fully the superiority of time and labour saving.
Claims (5)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN971098158A CN1064811C (en) | 1997-04-28 | 1997-04-28 | Application method of direct seading rice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN971098158A CN1064811C (en) | 1997-04-28 | 1997-04-28 | Application method of direct seading rice |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1166267A CN1166267A (en) | 1997-12-03 |
| CN1064811C true CN1064811C (en) | 2001-04-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN971098158A Expired - Fee Related CN1064811C (en) | 1997-04-28 | 1997-04-28 | Application method of direct seading rice |
Country Status (1)
| Country | Link |
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| CN (1) | CN1064811C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107926552B (en) * | 2017-11-20 | 2019-08-09 | 湖南杂交水稻研究中心 | A planting method capable of removing seedlings and weeds in paddy fields or seed production fields |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87106653A (en) * | 1986-09-26 | 1988-04-13 | 法伊特股份有限公司 | Seam-ironing plant |
| CN87106531A (en) * | 1986-08-26 | 1988-07-06 | 纳幕尔杜邦公司 | Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase |
| CN1036120A (en) * | 1987-11-17 | 1989-10-11 | 三井东圧化学株式会社 | A method for cultivating hybrid rice seeds |
| EP0461355A2 (en) * | 1990-05-09 | 1991-12-18 | American Cyanamid Company | A method for the prevention of crop injury in the presence of synergistic pesticide combinations |
-
1997
- 1997-04-28 CN CN971098158A patent/CN1064811C/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87106531A (en) * | 1986-08-26 | 1988-07-06 | 纳幕尔杜邦公司 | Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase |
| EP0730030A1 (en) * | 1986-08-26 | 1996-09-04 | E.I. Du Pont De Nemours And Company | Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase |
| CN87106653A (en) * | 1986-09-26 | 1988-04-13 | 法伊特股份有限公司 | Seam-ironing plant |
| CN1036120A (en) * | 1987-11-17 | 1989-10-11 | 三井东圧化学株式会社 | A method for cultivating hybrid rice seeds |
| EP0461355A2 (en) * | 1990-05-09 | 1991-12-18 | American Cyanamid Company | A method for the prevention of crop injury in the presence of synergistic pesticide combinations |
Non-Patent Citations (1)
| Title |
|---|
| 中国农业科学，29 6 1996.1.1 朱冰，黄大年等，利用基因枪法获得可遗传的抗除草剂转基因水稻植株 * |
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| Publication number | Publication date |
|---|---|
| CN1166267A (en) | 1997-12-03 |
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