CN106472817B - Bird's nest polypeptide and preparation method thereof - Google Patents
Bird's nest polypeptide and preparation method thereof Download PDFInfo
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- CN106472817B CN106472817B CN201610792966.2A CN201610792966A CN106472817B CN 106472817 B CN106472817 B CN 106472817B CN 201610792966 A CN201610792966 A CN 201610792966A CN 106472817 B CN106472817 B CN 106472817B
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/348—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of proteins obtained from waste materials
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/10—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from hair, feathers, horn, skins, leather, bones, or the like
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/012—Hydrolysed proteins; Derivatives thereof from animals
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Abstract
The invention provides a bird's nest polypeptide, its weight average molecular weight is smaller than 2000 Da; sialic acid content greater than 7% by weight. The invention also provides a preparation method and application of the bird's nest polypeptide. The bird's nest polypeptide provided by the invention is easy to digest and absorb in a human body, can quickly beautify and care the skin and improve sub-health symptoms, and has the advantages of simple and convenient preparation process, low production cost and good industrial application prospect.
Description
Technical Field
The invention belongs to the technical field of bird's nest processing, and particularly relates to bird's nest polypeptide and a preparation method thereof.
Background
Nidus Collocaliae, including Swallow, Yangen, and Swallow vegetable refer to nidus formed by mixing saliva and down feather of part of Asteris Latifolius of Asteris of Asteriales and several species of Asteris Latifolius of Asteris. The bird's nest is neutral in nature and sweet in taste, can tonify lung and yin, has the effects of nourishing yin and moistening dryness, tonifying qi and strengthening middle warmer, can relieve symptoms such as cough and hemoptysis, is praised as a nourishing sample from the Tang Dynasty, and is a traditional Chinese medicine sample which is equivalent to pilose antler, ginseng and the like.
The cubilose is rich in nutrient substances, including protein, polysaccharide, lipid, trace elements, sialic acid and the like. The protein is the main component, the content of the protein in the cubilose is up to more than 50%, the protein in the cubilose exists in a glycoprotein form, and the molecular weight is mainly distributed in 20-150 kDa. Sialic acid is an important active ingredient in the cubilose and has important regulation effect on physiological and biochemical functions of a human body. The edible bird's nest has the highest sialic acid content in nature, and the sialic acid content is more than 10%. Sialic acid is present in the bird's nest as a sialoglycoprotein.
At present, in the industries of nutrition and cosmetics, the traditional treatment of the cubilose is mainly a cooking rough processing mode, the cubilose sample obtained by the processing mode basically keeps the original macromolecular protein structure, and the absorption efficiency of the cubilose is lower because the molecular weight of the protein is too large to be completely absorbed by a human body, so that the proper protein modification method is very necessary for improving the absorption and utilization of the protein in the cubilose.
Several documents for modifying bird's nest with protein are disclosed in the present publication, for example, patent publication No. CN1858225A, which discloses a method for degrading bird's nest with ficin to obtainBird's nest polypeptide with molecular weight distribution of 200-1000D is shown, but the content ratio of sialic acid in the extract is not mentioned; the patent publication No. CN104498572A discloses a compound enzymolysis processing technology of bird's nest, the enzymolysis is realized by adding animal protease, neutral protease and flavor enzyme at the same time, the sialic acid content in the obtained bird's nest polypeptide sample is 10.5%, but the number average molecular weight of the product is 5.83 x 103Weight average molecular weight of 1.89 x 104The absorption and availability of the product in the human body is still not ideal.
With the enhancement of health care consciousness of people, the consumption demand of the cubilose is increasingly vigorous, and in order to meet the nutritional and functional demands of people on the cubilose, a more appropriate enzymolysis method is found to obtain small molecular active polypeptide, so that the nutritional value of the cubilose is very necessary to be fully exerted.
Disclosure of Invention
The invention aims to provide a bird's nest polypeptide containing small molecular active polypeptide and a preparation method thereof.
The invention provides a bird's nest polypeptide, the weight average molecular weight of the bird's nest polypeptide is less than 2000 Da; sialic acid content greater than 7% by weight.
Wherein the nidus Collocaliae polypeptide is prepared from nidus Collocaliae;
and/or the weight average molecular weight of the bird's nest polypeptide is 400-1500 Da, and the weight percentage content of sialic acid is 7-12%.
The invention also provides a preparation method of the bird's nest polypeptide, which comprises the following steps:
(a) extracting nidus Collocaliae with water to obtain nidus Collocaliae protein mother liquor;
(b) adjusting the temperature of the cubilose protein mother liquor obtained in the step (a) to 30-65 ℃, adjusting the pH value to 8-9, adding 1-8 parts by weight of alkaline protease, and reacting for 0.5-12 hours;
(c) adding 1-8 parts by weight of neutral protease at the temperature of 30-65 ℃, and reacting for 0.5-12 hours;
(d) adding 1-8 parts by weight of carbohydrase at the temperature of 40-55 ℃, and reacting for 0.5-12 hours;
(e) enzyme inactivation;
(f) filtering, and concentrating the filtrate.
The bird nest is derived from nests of several birds of the genus swiftlet: edible bird's nest.
The bird's nest is derived from Manyprickle bird's nest made in Malaysia and domestic golden wire bird's nest.
Wherein, in the step (a), the weight percentage content of the protein in the cubilose is not less than 50 percent, and the content of the nitrite is less than 2 ppm.
Wherein, in the step (a), the number of the water-adding extractions is as follows: taking 100 parts by weight of cubilose, adding 800-1200 parts by volume of water, adjusting the temperature to 70-85 ℃, and reacting for 20-60 minutes;
further, the number of the water-added extractions is as follows: 100 parts by weight of cubilose is taken, 800 parts by volume of water is added, the temperature is adjusted to 70 ℃, and the reaction is carried out for 20 minutes.
The relationship between parts by weight and parts by volume is "1 g to 1 mL".
Wherein in step (c) and/or (d), stirring is performed during the reaction;
in the step (d), before adding carbohydrase, adjusting the pH value to 5.5-6;
in step (e), the enzyme inactivation is performed by: adjusting the temperature to 75-90 ℃, and keeping the temperature for 15-30 minutes; preferably, the enzyme inactivation employs: adjusting the temperature to 80 ℃, and keeping the temperature for 15 minutes;
in the step (f), the concentration adopts vacuum spray drying or freeze vacuum drying.
Wherein, after the step (f), a sterilization step is also included.
Wherein the alkaline protease is at least one of alcalase or pancreatin; the neutral protease is at least one of A1398, Neutrase and Flavourzyme; the carbohydrase is at least one of lysozyme, cellulase and chitosanase.
The invention also provides application of the bird's nest polypeptide in preparation of food samples, food-like additives, health-care food samples and/or medicine samples.
Wherein the health food sample and the medicine sample are health food samples and medicine samples for beautifying and protecting skin, improving sub-health, enhancing immunity and promoting wound repair.
The bird's nest polypeptide prepared by the invention can exert the biological efficacy of protein and polysaccharide to the maximum extent, is easy to digest and absorb in human bodies, can quickly maintain beauty and protect skin, improves sub-health symptoms, and has better effect than the market sample production; the bird's nest polypeptide of the invention has simple preparation process and low production cost, can be used for preparing food samples, food sample additives, health food samples and medicine samples, is also suitable for the cosmetic sample industry, and has good industrial application prospect.
Obviously, many modifications, substitutions and variations of the present invention may be made to the above-described embodiments without departing from the basic technical concept of the invention in light of the common technical knowledge and conventional means in the art.
The present invention will be described in further detail with reference to the following examples. This should not be understood as limiting the scope of the above-described subject matter of the present invention to the following examples. All the technologies realized based on the above contents of the present invention belong to the scope of the present invention.
Drawings
FIG. 1 is a Malaysia bird's nest polypeptide map of the present invention;
FIG. 2 is a high performance liquid chromatography of sialic acid in bird's nest;
FIG. 3 is a liquid phase map of the domestic bird's nest polypeptide of the present invention.
Detailed Description
The following examples are further illustrative, but the present invention is not limited to these examples.
The process flow of the invention is as follows:
raw material-shaped edible bird's nest rough sample → cleaning → enzymolysis → inactivation enzyme → filtration → spray drying (or freeze drying) → sterilization;
or, the raw material-shaped edible bird's nest is roughly shaped → cleaned → enzymolyzed → inactivated enzyme → filtered → spray dried (or freeze dried).
The experimental reagents used in the present invention were as follows:
amylase, cellulase, chitosanase: all purchased from AB company, germany;
alcalase, pancreatin, Neutrase, Flavourzyme: all purchased from novicent;
a1398: purchased from general enzyme preparations, Beijing House county.
The detection method of each component is as follows:
firstly, a sialic acid detection method:
the instrument inspection equipment comprises:
shimadzu CBM-10A VP PLUS liquid chromatograph (ultraviolet detector: SPD-10A VP PLUS, infusion pump: LC-10AVP PLUS), KQ-300DE ultrasonic apparatus (Kunshan ultrasonic apparatus, Inc.), FA2004N electronic balance (Shanghai Cyanin sea apparatus, Inc., d 0.1mg), HH-6 constant temperature water bath (Korea electric inspection, Inc.), 10mL headspace sample injection bottle, C18 chromatographic column.
(II) reagent: standard sample sialic acid (N-acetylneuraminic acid): SIGMA-ALDRICH, 100mg, o-phenylenediamine hydrochloride, analytical grade, sodium bisulfate, analytical grade.
(III) sample preparation: taking bird's nest zymolyte samples and 10mg of standard samples, adding 1ml of sodium bisulfate solution with 0.5 molar concentration, carrying out water bath heating reaction for 30 minutes at 80 ℃, taking out and cooling to obtain hydrolysis sample solution. Taking the solution, adding 1ml of 10mg/ml o-phenylenediamine solution (dissolved by 0.25 molar sodium bisulfate solution), placing in 80 ℃ water bath for heating for 40 minutes, taking out and cooling, adding water to reach a constant volume of 10ml, and filtering to obtain the product.
(IV) the operation method comprises the following steps: number of chromatographic strips: 4.6mm × 250 mm; taking 1% tetrahydrofuran water solution (containing 0.2% phosphoric acid) -acetonitrile (92:8) as a mobile phase; the detection wavelength was 230nm, the flow rate was 1.0ml/min, and the injection volume was 20. mu.l.
Preparation of control standard solutions: a sialic acid (N-acetylneuraminic acid) standard sample (2 mg) was precisely weighed and treated in the same manner as the test sample to prepare a control sample solution (about 0.2 mg/ml).
And (3) verification: and respectively sucking 20 microliters of the control sample solution and the zymolyte sample solution, and injecting the solutions into a high performance liquid chromatograph for verification.
(V) a calculation method:
calculating the formula:
in the formula:
Sfor supplying to: the peak area of a main peak in a chromatogram of a test solution; sTo pair: the peak area of a main peak in a chromatogram of a reference solution;
mto pair: sample weight (mg) of control; pTo pair: percentage content of reference substance; m isFor supplying to: sample weighing (mg) of the test sample;
Vto pair: dilution times of reference substances; vFor supplying to: dilution factor (V) of test sampleTo pair＝VFor supplying to＝10))
II, a molecular weight detection method: indantrione method for detecting hydrolysis degree and molecular weight
(I) instrument detection & reagent
Detection by Beijing Pujingyu TU-1900 type ultraviolet-visible spectrophotometer or similar instrument
Pipette (2ml)
Liquid-transfering gun
Volumetric flask (100mL, 50mL)
Colorimetric tube with plug (10ml)
Ninhydrin, glycine, fructose, disodium hydrogen phosphate, potassium dihydrogen phosphate (analytically pure)
Ethanol
(II) Experimental procedure
1. 20 μ g/mL glycine standard solution: 2mg glycine was metered into a 100mL volumetric flask.
2. Ninhydrin indicator formulation: 0.5g of ninhydrin, 0.3g of fructose, 11.2g of disodium hydrogen phosphate and 6g of potassium dihydrogen phosphate, wherein the volume is 100mL, the ninhydrin is dissolved in heat, and the ninhydrin can be stored at low temperature for 1 week.
3. Drawing a standard curve: taking 9 test tubes with plugs, precisely absorbing standard solutions according to the following table to 10mL test tubes with plugs, adding pure water to 2.0mL each, adding ninhydrin color developing agent to 1.0mL, uniformly mixing, heating in boiling water bath for 15min, rapidly cooling to room temperature with cold water, adding 40% ethanol to 5mL, shaking uniformly, standing for 15min, and detecting the absorbance (A) at a wavelength of 570nm as an ordinate and the content as an abscissa to prepare a standard curve.
4. Sample assay
4.1 sample solution: taking 0.50mL of hydrolysate to fix the volume to 50 mL.
4.2 sample assay: sucking 0.5mL of sample solution, placing the sample solution into a colorimetric tube with a plug (each sample is repeated for 2 times), adding pure water to 2.0mL, adding ninhydrin color developing agent to 1.0mL, uniformly mixing, heating in a boiling water bath for 15min, then rapidly cooling to room temperature with cold water, adding 40% ethanol to 5mL, shaking up, standing for 15min, carrying out color comparison at 570nm, using a test tube No. 0 as a reference, detecting absorbance, and checking the content through a standard curve.
Total millimoles of peptide bonds (i.e. the sum of millimoles of peptide bonds)
4.3 results calculation:
hydrolysate-NH2The content (mu mol/mL) is [ C/(75X V)]*100
In the formula, N-hydrolysate protein content (Kjeldahl nitrogen determination)
htotMillimoles of peptide bonds per gram of starting protein
C-content of standard curve found (μ g)
Molecular weight of 75-Glycine
V-volume of sample solution to be examined
100-dilution factor of sample
4.4 assay of average Peptide Chain Length (PCL) and average molecular weight (Mw):
PCL＝1/DH
Mw＝110*PCL+18
example 1 preparation of bird's nest polypeptide of the invention
1. Raw materials: imported Malaysia bird's nest.
2. The preparation method comprises the following steps:
washing 100 g of coarse bird's nest sample with water, adding 1000 ml of deionized water, heating to 85 ℃ for 30 minutes, then cooling to 45 ℃, adjusting the pH value to 8, adding 3g of alcalase, degrading for 2 hours, adding 4 g of A1398 enzyme under the same number, reacting for 2 hours, dissolving the bird's nest to form white semitransparent reaction liquid, then adjusting the pH value to 6, adding lysozyme, reacting for 2 hours at 40 ℃, heating to 80 ℃, preserving the temperature for 15 minutes, inactivating the enzyme, and filtering while the bird's nest is hot. And distilling the supernatant under reduced pressure at the vacuum degree of 0.06-0.10 Mpa and the temperature of 40-65 ℃, and finally freeze-drying to obtain the cubilose polypeptide.
3. And (3) detection results:
(1) polypeptide weight average molecular weight: 860 Da.
(2) Sialic acid content detection results: sialic acid 8.4%.
(3) Total content of amino acids: 50.63 percent. The contents of the individual amino acids are shown in Table 1.
TABLE 1 amino acid content of bird's nest polypeptides of the invention
| Amino acids | Bird's nest polypeptide (i.e. bird's nest zymolyte 201406) |
| Aspartic acid | 5.16 |
| Threonine | 3.86 |
| Serine | 4.32 |
| Glutamic acid | 4.00 |
| Glycine | 2.22 |
| Alanine | 1.57 |
| Cystine | 1.45 |
| Valine | 3.87 |
| Methionine | 0.44 |
| Isoleucine | 1.59 |
| Leucine | 3.99 |
| Tyrosine | 3.10 |
| Phenylalanine | 3.40 |
| Lysine | 2.06 |
| Histidine | 2.09 |
| Arginine | 4.11 |
| Proline | 3.40 |
| Total amount of amino acids | 50.63 |
Example 2 preparation of bird's nest polypeptide of the invention
1. Raw materials: imported Malaysia bird's nest.
2. The preparation method comprises the following steps:
the method comprises the steps of washing 100 g of crude bird's nest sample with water, adding 1200 ml of deionized water, heating to 80 ℃ for 60 minutes, cooling to 65 ℃, adjusting the pH value to 9, adding 8 g of pancreatin, degrading for 12 hours, adding 4 g of Neutrase enzyme under the same number, reacting for 12 hours, dissolving the bird's nest to form white semitransparent reaction liquid, adding lysozyme, reacting for 12 hours at 40 ℃, heating to 90 ℃, preserving heat for 30 minutes, inactivating the enzyme, and filtering while the bird's nest is hot. And distilling the supernatant under reduced pressure at the vacuum degree of 0.06-0.10 Mpa and the temperature of 40-65 ℃, and finally freeze-drying to obtain the cubilose polypeptide.
3. And (3) detection results:
(1) polypeptide weight average molecular weight: 956 Da.
(2) Sialic acid content detection results: sialic acid 9.2%.
(3) Total content of amino acids: 46.46 percent. The contents of the individual amino acids are shown in Table 2.
The polypeptide spectrogram of the bird's nest is shown in figure 1, and the high performance liquid chromatogram of sialic acid in the bird's nest is shown in figure 2.
TABLE 2 amino acid content of bird's nest polypeptide of the invention
| Amino acids | Bird's nest polypeptide |
| Aspartic acid | 4.81 |
| Threonine | 3.52 |
| Serine | 3.84 |
| Glutamic acid | 3.51 |
| Glycine | 1.99 |
| Alanine | 1.54 |
| Cystine | 1.27 |
| Valine | 3.76 |
| Methionine | 0.42 |
| Isoleucine | 1.58 |
| Leucine | 3.65 |
| Tyrosine | 2.63 |
| Phenylalanine | 3.14 |
| Lysine | 1.96 |
| Histidine | 1.78 |
| Arginine | 3.36 |
| Proline | 3.7 |
| Total amount of amino acids | 46.46 |
Example 3 preparation of bird's nest polypeptide of the invention
1. Raw materials: imported Malaysia bird's nest.
2. The preparation method comprises the following steps:
100 g of crude bird's nest sample is washed by water, 800 ml of deionized water is added, the temperature is raised to 70 ℃ for 20 minutes, then the temperature is lowered to 30 ℃, the pH value is adjusted to 8, 1g of alcalase is added, the degradation is carried out for 0.5 hour, 1g of Flavourzyme enzyme is added under the same number of pieces, the reaction is carried out for 0.5 hour, the bird's nest is dissolved to be white semitransparent reaction liquid, lysozyme is added, the reaction is carried out for 0.5 hour at the temperature of 40 ℃, then the temperature is raised to 75 ℃, the temperature is kept for 15 minutes, the enzyme is inactivated, and the filtration is carried out while the. And distilling the supernatant under reduced pressure at the vacuum degree of 0.06-0.10 Mpa and the temperature of 40-65 ℃, and finally freeze-drying and sterilizing to obtain the bird's nest polypeptide.
3. The result of the detection
(1) Polypeptide weight average molecular weight: 1012 Da.
(2) Sialic acid content detection results: sialic acid 10.3%.
(3) Total content of amino acids: 56.02 percent. The contents of the individual amino acids are shown in Table 3.
TABLE 3 amino acid content of bird's nest polypeptide of the invention
| Amino acids | Bird's nest polypeptide |
| Aspartic acid | 5.34 |
| Threonine | 3.95 |
| Serine | 4.37 |
| Glutamic acid | 3.94 |
| Glycine | 2.16 |
| Alanine | 1.77 |
| Cystine | 1.65 |
| Valine | 3.91 |
| Methionine | 0.47 |
| Isoleucine | 1.74 |
| Leucine | 4.22 |
| Tyrosine | 3.84 |
| Phenylalanine | 3.77 |
| Lysine | 2.21 |
| Histidine | 2.01 |
| Arginine | 3.89 |
| Proline | 4.86 |
| Tryptophan | 0.6 |
| Total amount of amino acids | 56.02 |
Example 4 preparation of bird's nest polypeptide of the invention
1. Raw materials: domestic edible bird's nest.
2. The preparation method comprises the following steps:
weighing 100 g of dry sample crushed cubilose in a 1000 ml beaker, heating to 80 ℃, keeping the temperature for 30 minutes, cooling to 50 ℃ after swelling, adjusting the pH value to 8, adding 2.5 g of pancreatin, and reacting for 3 hours; adding 4 g of A1398 enzyme under the same number, reacting for 3 hours, dissolving the bird's nest into white semitransparent reaction liquid, then adjusting the pH value to 5.5, adding 1-8 g of cellulase, reacting for 3 hours at 55 ℃, then heating to 80 ℃, preserving the temperature for 15 minutes, inactivating the enzyme, and filtering while the solution is hot. And distilling the supernatant under reduced pressure at the vacuum degree of 0.06-0.10 Mpa and the temperature of 40-65 ℃, and finally freeze-drying to obtain the cubilose polypeptide.
3. And (3) detection results:
(1) yield: 66.7 percent
(2) Weight average molecular weight: 988 Da.
(3) Sialic acid content detection results: sialic acid 7.12%.
(4) Total content of amino acids: 46.46 percent. The contents of the individual amino acids are shown in Table 4.
The bird's nest polypeptide spectrogram of the invention is shown in figure 3.
TABLE 4 amino acid content of bird's nest polypeptide of the invention
| Amino acids | Bird's nest polypeptide (bird's nest zymolyte 20140814) |
| Aspartic acid | 5.18 |
| Threonine | 3.79 |
| Serine | 3.95 |
| Glutamic acid | 3.78 |
| Glycine | 2.19 |
| Alanine | 1.67 |
| Cystine | 1.32 |
| Valine | 3.99 |
| Methionine | 0.27 |
| Isoleucine | 1.72 |
| Leucine | 4.07 |
| Tyrosine | 3.59 |
| Phenylalanine | 3.62 |
| Lysine | 2.06 |
| Histidine | 1.88 |
| Arginine | 3.49 |
| Proline | 3.81 |
| Tryptophan | 0.84 |
| Total amount of amino acids | 52.29 |
Example 5 preparation of bird's nest polypeptide of the invention
1. Raw materials: domestic edible bird's nest.
2. The preparation method comprises the following steps:
weighing 100 g of dry sample crushed cubilose in a 1200 ml beaker, heating to 70 ℃, keeping the temperature for 20 minutes, cooling to 30 ℃ after swelling, adjusting the pH value to 8, adding 1g of pancreatin, and reacting for 0.5 hour; under the same number, 8 g of Neutrase enzyme is added for reaction for 12 hours, the bird's nest is dissolved into white semitransparent reaction liquid, then the pH value is adjusted to 6, 8 g of cellulase is added for reaction for 12 hours at 40 ℃, then the temperature is raised to 90 ℃, the temperature is kept for 15 minutes, the enzyme is inactivated, and the filtration is carried out when the reaction liquid is hot. And distilling the supernatant under reduced pressure at the vacuum degree of 0.06-0.10 Mpa and the temperature of 40-65 ℃, and finally freeze-drying to obtain the cubilose polypeptide.
3. And (3) detection results:
(1) yield: 68.2 percent of
(2) Weight average molecular weight: 1130 Da.
(3) Sialic acid content detection results: sialic acid 9.87%.
(4) Total content of amino acids: 52.67 percent. The contents of the individual amino acids are shown in Table 5.
TABLE 5 amino acid content of bird's nest polypeptide of the invention
| Amino acids | Bird's nest polypeptide (namely bird's nest zymolyte 2015 years) |
| Aspartic acid | 5.25 |
| Threonine | 3.89 |
| Serine | 4.31 |
| Glutamic acid | 3.94 |
| Glycine | 2.2 |
| Alanine | 1.66 |
| Cystine | 1.28 |
| Valine | 3.87 |
| Methionine | 0.32 |
| Isoleucine | 1.78 |
| Leucine | 4.1 |
| Tyrosine | 4.03 |
| Phenylalanine | 3.51 |
| Lysine | 2.07 |
| Histidine | 1.91 |
| Arginine | 3.7 |
| Proline | 3.96 |
| Total amount of amino acids | 52.67 |
The beneficial effects of the invention are illustrated by way of test examples as follows:
test example 1 Effect test of bird's nest polypeptide of the present invention
The bird's nest polypeptide prepared in example 3 is used for the effect experiment of beautifying and protecting skin and improving sub-health, and the result is shown in table 6:
TABLE 6 comparison of the Effect of the bird's nest polypeptide of the present invention and that of commercially available bird's nests
As can be seen from Table 6, the bird's nest polypeptide of the present invention has good effects of beautifying and caring skin and improving sub-health symptoms, and is especially suitable for middle-aged and elderly women. Compared with the commercially available cubilose, the cubilose polypeptide has quick response and obvious advantages.
In conclusion, the bird's nest polypeptide provided by the invention can exert biological effects of protein and polysaccharide to the maximum extent, is easy to digest and absorb in a human body, can quickly beautify and protect skin, improves sub-health symptoms, and has an effect superior to that of a commercially available product; the bird's nest polypeptide of the invention has simple preparation process, low production cost and good industrial application prospect.
Claims (7)
1. A bird's nest polypeptide, characterized by:
the weight average molecular weight of the bird's nest polypeptide is 400-1500 Da, and the weight percentage content of sialic acid is 7-12%;
the preparation method of the bird's nest polypeptide comprises the following steps:
(a) taking 100 parts by weight of cubilose, adding 800-1200 parts by volume of water, adjusting the temperature to 70-85 ℃, and reacting for 20-60 minutes to obtain cubilose protein mother liquor;
(b) adjusting the temperature of the cubilose protein mother liquor obtained in the step (a) to 30-65 ℃, adjusting the pH value to 8-9, adding 1-8 parts by weight of alkaline protease, and reacting for 0.5-12 hours;
(c) adding 1-8 parts by weight of neutral protease at the temperature of 30-65 ℃, and reacting for 0.5-12 hours;
(d) adding 1-8 parts by weight of carbohydrase at the temperature of 40-55 ℃, and reacting for 0.5-12 hours;
(e) enzyme inactivation;
(f) filtering, and concentrating the filtrate;
in the step (a), the weight percentage content of protein in the cubilose is not less than 50%, and the content of nitrite is less than 2 ppm;
the alkaline protease is at least one of alcalase or pancreatin; the neutral protease is at least one of A1398, Neutrase and Flavourzyme; the carbohydrase is at least one of lysozyme, cellulase and chitosanase.
2. The bird's nest polypeptide of claim 1, characterized in that: in the step (a), the conditions of adding water for extraction are as follows: 100 parts by weight of cubilose is taken, 800 parts by volume of water is added, the temperature is adjusted to 70 ℃, and the reaction is carried out for 20 minutes.
3. The bird's nest polypeptide of claim 1, characterized in that: in the step (c) and/or (d), stirring is carried out during the reaction;
in the step (d), before adding carbohydrase, adjusting the pH value to 5.5-6;
in step (e), the enzyme inactivation is performed by: adjusting the temperature to 75-90 ℃, and preserving the heat for 15-30 minutes.
4. The bird's nest polypeptide of claim 3, characterized by: in step (e), the enzyme inactivation is performed by: the temperature was adjusted to 80 ℃ and the incubation was continued for 15 minutes.
5. The bird's nest polypeptide of claim 1, characterized in that: after step (f), a sterilization step is also included.
6. Use of the bird's nest polypeptide of any one of claims 1 to 5 in the preparation of food, food additive, health food and/or medicine.
7. The use according to claim 6, wherein the health food or medicine is a health food or medicine for caring skin, improving sub-health, enhancing immunity, and promoting wound repair.
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| CN107411069A (en) * | 2017-07-27 | 2017-12-01 | 杭州玖拾伍度鲜生物科技有限公司 | The cubilose product and its preparation technology of a kind of low temperature process |
| CN107485029A (en) * | 2017-07-27 | 2017-12-19 | 杭州玖拾伍度鲜生物科技有限公司 | One kind liquefaction cubilose product and its preparation technology |
| CN107668312B (en) * | 2017-11-22 | 2020-12-29 | 福建农林大学 | A kind of integrated defoaming method of bird's nest protein liquid |
| CN108371324A (en) * | 2018-01-18 | 2018-08-07 | 浙江燕宝丽堂电子商务有限公司 | A kind of preparation process of bird's nest essence oral solution |
| CN108148880B (en) * | 2018-03-02 | 2021-10-08 | 姚之龙 | Method for preparing sialic acid and oligopeptide mixture by using cubilose as raw material |
| CN110257459B (en) * | 2019-05-16 | 2021-04-13 | 广西慧宝源医药科技有限公司 | Bird's nest small molecular peptide and instant peptide powder thereof |
| CN111820412A (en) * | 2020-08-10 | 2020-10-27 | 广西自贸区燕握生物科技有限公司 | Method for extracting cubilose, extract and product thereof |
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