CN106405100A - Anti-proliferating cell nuclear antigen (PCNA) antibody quantitative kit, making method and use method thereof - Google Patents
Anti-proliferating cell nuclear antigen (PCNA) antibody quantitative kit, making method and use method thereof Download PDFInfo
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- 102000009339 Proliferating Cell Nuclear Antigen Human genes 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 16
- 102000019040 Nuclear Antigens Human genes 0.000 title claims abstract description 9
- 108010051791 Nuclear Antigens Proteins 0.000 title claims abstract description 9
- 230000001028 anti-proliverative effect Effects 0.000 title claims abstract description 9
- 239000000758 substrate Substances 0.000 claims abstract description 34
- 108010001336 Horseradish Peroxidase Proteins 0.000 claims abstract description 22
- 238000002965 ELISA Methods 0.000 claims abstract description 20
- 108090001008 Avidin Proteins 0.000 claims abstract description 18
- 238000002835 absorbance Methods 0.000 claims abstract description 9
- 239000007790 solid phase Substances 0.000 claims abstract description 8
- YRNWIFYIFSBPAU-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]-n,n-dimethylaniline Chemical compound C1=CC(N(C)C)=CC=C1C1=CC=C(N(C)C)C=C1 YRNWIFYIFSBPAU-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 48
- 239000007788 liquid Substances 0.000 claims description 46
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 26
- 238000011002 quantification Methods 0.000 claims description 19
- 238000007865 diluting Methods 0.000 claims description 14
- 239000011616 biotin Substances 0.000 claims description 13
- 229960002685 biotin Drugs 0.000 claims description 13
- 235000020958 biotin Nutrition 0.000 claims description 13
- 239000012224 working solution Substances 0.000 claims description 11
- 239000012895 dilution Substances 0.000 claims description 10
- 238000010790 dilution Methods 0.000 claims description 10
- 238000011088 calibration curve Methods 0.000 claims description 9
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 8
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 8
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 8
- 235000011149 sulphuric acid Nutrition 0.000 claims description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims description 7
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- 238000002360 preparation method Methods 0.000 claims description 5
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- 235000011330 Armoracia rusticana Nutrition 0.000 description 1
- 240000003291 Armoracia rusticana Species 0.000 description 1
- 108010013534 Auxilins Proteins 0.000 description 1
- 102000016736 Cyclin Human genes 0.000 description 1
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6875—Nucleoproteins
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
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Abstract
The invention relates to an anti-proliferating cell nuclear antigen (PCNA) antibody quantitative kit, a making method and a use method thereof. According to the kit, a purified antibody is employed to coat an ELISA plate to make a solid phase carrier, a specimen or standard substance, biotinylated anti-PCNA antibody and horseradish peroxidase labeled avidin can be added into the ELISA plate coating the anti-PCNA antibody, and washing with a scrub solution is carried out thoroughly, and then a substrate solution is employed for color development. Tetramethyl benzidine (TMB) turns into blue under the catalysis of horseradish peroxidase, and turns into yellow finally under the action of a stopping solution. The depth of color is positively correlated with the proliferating cell nuclear antigen (PCNA) in the sample. An ELISA detector is taken to determine the absorbance (OD value) and calculate the sample concentration. The detection kit provided by the invention has the advantages of convenient use, high detection efficiency, accuracy and simplicity, and is suitable for qualitative or quantitative analysis of batch samples.
Description
Technical field
The present invention relates to the quantitative determination of anti-proliferating cell nuclear antigen antibody is and in particular to a kind of PCNA
(PCNA) antibody quantification kit and preparation method thereof and using method.
Background technology
PCNA (proliferating cell nuclear antigen, PCNA) also known as Cyclin or
Archaeal dna polymerase δ auxilin, is the nuclear protein polypeptide of a 36KD.Myachi in 1978 etc. finds systemic erythema first
Autoantibody (antinuclear antibodies) in lupus patient serum and nucleus vegetative state have substantial connection.PCNA is in cell proliferation week
Interim effect is：Be combined with archaeal dna polymerase δ, thus guiding completes the duplication of DNA.When PCNA lacks, after DNA
Synthesize with short DNA fragmentation is only had on the template of chain；When PCNA is when archaeal dna polymerase δ is combined, guiding chain is synthesized,
So that being synthesized of can coordinating of DNA double chain.Thus archaeal dna polymerase δ and PCNA may be responsible for synthesizing guiding chain.When
When the few de- nucleotides of the antisense of PCNA acts on cell, the synthesis of DNA and the mitosis of cell are completely suppressed.Thus entering one
Step confirms important function in terms of cell DNA synthesis and cell cycle progress for the PCNA.
Different phase in cell generation cycle for the expression of PCNA is different.In resting stage and G1 early stage, PCNA is several
Do not express；The expression of G1 late period PCNA starts to increase；Peak in the S phase, subsequently reduce in G2 phase and M phase.PCNA sends out
Now with its monoclonal antibody be established as studying normal and abnormal cell propagation in vivo and changing provide new research meanses.
Expression due to PCNA has direct relation with the synthesis of DNA, and its presence directly shows that cell is in proliferating cycle, thus having
Help accurately understand pathogenic process and the progress of various kidney troubles.Because PCNA is in nucleus, its SABC
Dyeing can be joined together with the dyeing of other labels (identification endochylema or endochylema antigen) of cell, using Double Labelling Technique
Can accurately differentiate that the cell being in proliferating cycle is that PCNA positive cell belongs to that cell type, thus it is each to contribute to analysis
Plant effect in kidney disease progression for the cell.
But still there is no fast and effectively method for Quantitation of Proliferating Cell Nuclear Antigen detection at present.
Content of the invention
For above prior art problem, it is an object of the invention to provide a kind of PCNA (PCNA) resists
Body quantification kit and preparation method thereof and using method, to solve the difficulty of Quantitation of Proliferating Cell Nuclear Antigen detection in prior art
Topic.The antibody coated elisa plate that PCNA (PCNA) antibody quantification kit purifies, makes solid phase carrier, past
Sample or standard items, biotinylated anti-are sequentially added in the ELISA Plate being coated anti-proliferating cell nuclear antigen antibody (PCNA) antibody
PCNA antibody, the Avidin of horseradish peroxidase-labeled, are developed the color with substrate solution after cleaning solution thoroughly washs.Tetramethyl
Benzidine (TMB) converts au bleu under the catalysis crossing horseradish peroxidase, and changes into final in the presence of terminate liquid
Yellow.PCNA (PCNA) in the depth and sample of color is proportionate.With ELIASA mensuration absorbance (OD
Value), calculate sample concentration.Concrete technical scheme is as follows：
A kind of PCNA (PCNA) antibody quantification kit, its solid phase carrier is that the antibody purifying is coated
ELISA Plate.
Further, the antibody of described purifying is anti-proliferating cell nuclear antigen antibody (PCNA) antibody.
Further, standard items, sample diluting liquid, biotin labelled antibodies dilution, horseradish peroxidase are also included
Mark Avidin dilution, biotin labelled antibodies, Horseradish peroxidase-conjugated avidin, substrate solution, dense cleaning solution with
And terminate liquid.
Further, described standard items are pulverulent solids or solution state.
Further, described standard items are solution form, and its concentration is respectively 0ug/L, 0.03ug/L, 0.06ug/L,
0.12ug/L, 0.24ug/L, 0.48ug/L.
Further, described terminate liquid is HCl or H2SO4.
Further, described substrate solution comprises the first substrate solution and the second substrate solution, described first substrate solution
For hydrogen peroxide, described second substrate solution is tetramethyl benzidine (TMB).
The preparation method of above-mentioned PCNA (PCNA) antibody quantification kit, is coated enzyme with the antibody purifying
Target, makes solid phase carrier；Sequentially add sample toward in the ELISA Plate being coated anti-proliferating cell nuclear antigen antibody (PCNA) antibody
Or standard items, biotinylated anti-PCNA antibody, the Avidin of horseradish peroxidase-labeled.
The using method of above-mentioned PCNA (PCNA) antibody quantification kit, comprises the steps：
(1) configuration standard liquid；
(2) it is loaded；
(3) discard liquid and dry, plus working solution；
(4) after incubating, discard in the hole liquid, dry；
(5) every hole adds Horseradish peroxidase-conjugated avidin working solution；
(6) after incubating, discard in the hole liquid, dry；
(7) sequentially every hole adds substrate solution；
(8) sequentially every hole adds stop bath；
(9) enzyme connection instrument measurement；Or further include：
(10) draw calibration curve.
Further, configure good all reagent before step (1) in advance, when reagent or Sample Dilution, be both needed to mix, mix
Shi Jinliang avoids bubbling；And/or, in step (1), dissolve standard items with sample diluting liquid, concentration be made into 0.48ug/L,
0.24ug/L, 0.12ug/L, 0.06ug/L, 0.03ug/L, 0ug/L, wherein 0ug/L are the sample diluting liquid without standard items；
And/or, in step (2), set blank well, gauge orifice, testing sample hole respectively, blank well adds sample diluting liquid 100ul, Yu Kongfen
Not plus standard items or testing sample 100ul, sample is added on ELISA Plate bottom hole portion by sample-adding, gently rocks mixing, ELISA Plate adds
Lid or overlay film, 37 DEG C are reacted 120 minutes；And/or, in step (3), every hole adds biotin labelled antibodies working solution 100ul, and 37
DEG C, 60 minutes；And/or, in step (4), after incubating 60 minutes, discard in the hole liquid, dry, wash plate 3 times, soak 1-2 every time
Minute, the every hole of 350ul/, dry；And/or, in step (5), every hole adds Horseradish peroxidase-conjugated avidin working solution
100ul, 37 DEG C, 60 minutes；And/or, in step (6), after incubating 60 minutes, discard in the hole liquid, dry, wash plate 5 times, every time
Soak 1-2 minute, the every hole of 350ul/, dry；And/or, in step (7), sequentially every hole adds substrate solution 90ul, and 37 DEG C of lucifuges show
Color；And/or, in step (8), sequentially every hole adds stop bath 50ul, terminating reaction；And/or, in step (9), existed with enzyme connection instrument
450nm wavelength sequentially measures the optical density in each hole, is detected after adding terminate liquid within 15 minutes；And/or, step (10)
In, draw calibration curve, and the absorbance after example reaction is contrasted with calibration curve, determine sample concentration, and be multiplied by dilution
Multiple, had both obtained raw sample concentration.
Compared with currently available technology, it can be numerous visitors that ELISA of the present invention measures PCNA (PCNA)
Family accepts, simple to operate, and standard items adopt powder solid, and client is just configured although being increased the behaviour of user using front
Make step, but concentration is accurately, is not in the situation of active degradation.The detection kit of the present invention is easy to use, detection side
Method is efficient, accurate, easy, and suitable batch samples are qualitative or quantitative.Specifically：Enzyme linked immunological is used as relatively more classical
Biology sample detection method, ELISA measures PCNA (PCNA) and can accept for numerous clients, simple to operate,
And standard items adopt powder solid, client is just configured although being increased the operating procedure of user using front, but concentration is accurate
Really, be not in active degradation situation.The detection kit of the present invention is easy to use, detection method is efficient, accurate, easy,
Suitable batch samples are qualitative or quantitative.
Brief description
For PCNA (PCNA) antibody standard substance canonical plotting
Specific embodiment
Describe the present invention below according to accompanying drawing, it is that one of numerous embodiments of the present invention are preferably real
Apply example.
In a preferred embodiment, a kind of PCNA (PCNA) antibody quantification kit, described reagent
The reaction principle of box is：With the antibody coated elisa plate purifying, make solid phase carrier, toward being coated anti-proliferating cell nuclear antigen antibody
(PCNA) sample or standard items, biotinylated anti-PCNA antibody, horseradish peroxidase are sequentially added in the ELISA Plate of antibody
The Avidin of mark, is developed the color with substrate solution after cleaning solution thoroughly washs.Tetramethyl benzidine (TMB) is crossing horseradish peroxide
Convert au bleu under the catalysis of compound enzyme, and change into final yellow in the presence of terminate liquid.The depth of color and sample
In PCNA (PCNA) be proportionate.With ELIASA mensuration absorbance (OD value), calculate sample concentration.
Kit comprises ELISA Plate, standard items, sample diluting liquid, biotin labelled antibodies dilution, horseradish peroxidase
Enzyme mark Avidin dilution, biotin labelled antibodies, Horseradish peroxidase-conjugated avidin, substrate solution, dense cleaning solution,
Terminate liquid.Described standard items are pulverulent solids or solution state, and if solution form, its concentration is respectively 0ug/L,
0.03ug/L, 0.06ug/L, 0.12ug/L, 0.24ug/L, 0.48ug/L.Preferably powder solid.Described terminate liquid be HCl or
H2SO4, preferably H2SO4, more preferably H2SO4 concentration are 4M.Described substrate solution comprises substrate solution 1 and substrate solution 2, described
Substrate solution 1 is hydrogen peroxide, and described substrate solution 2 is tetramethyl benzidine (TMB).Make during described ELIASA mensuration absorbance
It is 400～550nm with wavelength, optimal wavelength 450nm.
In a further advantageous embodiment, a kind of PCNA (PCNA) antibody quantification kit, further
Ground kit comprises ELISA Plate, standard items, sample diluting liquid, biotin labelled antibodies dilution, horseradish peroxidase-labeled
Avidin dilution, biotin labelled antibodies, Horseradish peroxidase-conjugated avidin, substrate solution, dense cleaning solution, termination
Liquid.
Described standard items are pulverulent solids or solution state, and if solution form, its concentration is respectively 0ug/L,
0.03ug/L, 0.06ug/L, 0.12ug/L, 0.24ug/L, 0.48ug/L.Preferably powder solid.
Described terminate liquid is HCl or H2SO4, preferably H2SO4, and more preferably H2SO4 concentration is 4M.Described substrate solution comprises
Substrate solution 1 and substrate solution 2, described substrate solution 1 is hydrogen peroxide, and described substrate solution 2 is tetramethyl benzidine
(TMB).
The reaction principle of described kit is：With the antibody coated elisa plate purifying, make solid phase carrier, toward being coated anti-increasing
Sequentially add sample in the ELISA Plate of cell colonization NA antibody (PCNA) antibody or standard items, biotinylated anti-PCNA resist
Body, the Avidin of horseradish peroxidase-labeled, are developed the color with substrate solution after cleaning solution thoroughly washs.Tetramethyl benzidine
(TMB) convert au bleu under the catalysis crossing horseradish peroxidase, and change into final yellow in the presence of terminate liquid.
PCNA (PCNA) in the depth and sample of color is proportionate.With ELIASA mensuration absorbance (OD value), count
Calculate sample concentration.
Wavelength is selected to be 400～550nm during described ELIASA mensuration absorbance, optimal wavelength 450nm.
Specific using method adopts following steps：
Before experiment starts, when please having configured all reagent, reagent or Sample Dilution in advance, it is both needed to mix, tries one's best during mixing
Avoid bubbling.Detection every time all should do calibration curve.When as too high in sample concentration, it is diluted with sample diluting liquid, so that
The detection range of samples met kit.
1st, configuration standard liquid：Dissolve standard items with sample diluting liquid, concentration is made into 0.48ug/L, 0.24ug/L, 0.12ug/
L, 0.06ug/L, 0.03ug/L, 0ug/L, wherein 0ug/L are the sample diluting liquid without standard items.
2nd, it is loaded：Set blank well, gauge orifice, testing sample hole respectively.Blank well adds sample diluting liquid 100ul, Yu Kongfen
Not plus standard items or testing sample 100ul, it has been careful not to bubble, sample is added on ELISA Plate bottom hole portion, does not touch as far as possible by sample-adding
And hole wall, gently rock mixing, ELISA Plate adds lid or overlay film, 37 DEG C are reacted 120 minutes.
3rd, discard liquid, dry, without washing.Every hole adds biotin labelled antibodies working solution 100ul and (takes 1ul biotin
Labelled antibody adds the proportions of 99ul biotin labelled antibodies dilution, gently mixes, and prepares in one hour before use),
37 DEG C, 60 minutes.
4th, after incubating 60 minutes, discard in the hole liquid, dry, wash plate 3 times, soak 1-2 minute every time, the every hole of 350ul/,
Dry.
5th, every hole adds Horseradish peroxidase-conjugated avidin working solution (with biotin labelled antibodies working solution) 100ul,
37 DEG C, 60 minutes.
6th, after incubating 60 minutes, discard in the hole liquid, dry, wash plate 5 times, soak 1-2 minute every time, the every hole of 350ul/,
Dry.
7th, sequentially every hole adds substrate solution 90ul, and 37 DEG C of lucifuges develop the color (in 30 minutes, now before naked eyes visual standard product
3-4 hole has obvious gradient blue, and rear 3-4 gradient pores are inconspicuous, you can terminate).
8th, sequentially every hole adds stop bath 50ul, terminating reaction (now blue standing turns yellow).The addition sequence of terminate liquid
Should try one's best identical with the addition sequence of substrate solution.In order to ensure the accuracy of experimental result, the substrate reactions time arrive after should be as early as possible
Add terminate liquid.
9th, join, with enzyme, the optical density (OD value) that instrument sequentially measures each hole in 450nm wavelength.Plus terminate liquid after 15 minutes with
Inside detected.
10th, draw calibration curve, and the absorbance after example reaction is contrasted with calibration curve, determine sample concentration.And
It is multiplied by extension rate, both obtained raw sample concentration.
Above in conjunction with accompanying drawing, the present invention is exemplarily described it is clear that the present invention implements is not subject to aforesaid way
Restriction, as long as employing method of the present invention design and the various improvement that carry out of technical scheme, or not improved direct application
In other occasions, all within protection scope of the present invention.
Claims (10)
1. a kind of PCNA (PCNA) antibody quantification kit is it is characterised in that its solid phase carrier is purify
The coated ELISA Plate of antibody.
2. PCNA (PCNA) antibody quantification kit as claimed in claim 1 is it is characterised in that described pure
The antibody changed is anti-proliferating cell nuclear antigen antibody (PCNA) antibody.
3. PCNA (PCNA) the antibody quantification kit as described in claim 1 and 2 is it is characterised in that go back
Including standard items, sample diluting liquid, biotin labelled antibodies dilution, Horseradish peroxidase-conjugated avidin dilution, life
Thing element labelled antibody, Horseradish peroxidase-conjugated avidin, substrate solution, dense cleaning solution and terminate liquid.
4. PCNA (PCNA) antibody quantification kit as claimed in claim 3 is it is characterised in that described mark
Quasi- product are pulverulent solids or solution state.
5. PCNA (PCNA) antibody quantification kit as claimed in claim 4 is it is characterised in that described mark
Quasi- product are solution form, and its concentration is respectively 0ug/L, 0.03ug/L, 0.06ug/L, 0.12ug/L, 0.24ug/L, 0.48ug/
L.
6. the PCNA as described in claim 3-5 (PCNA) antibody quantification kit is it is characterised in that described
Terminate liquid is HCl or H2SO4.
7. the PCNA as described in claim 3-5 (PCNA) antibody quantification kit is it is characterised in that described
Substrate solution comprises the first substrate solution and the second substrate solution, and described first substrate solution is hydrogen peroxide, described second bottom
Thing solution is tetramethyl benzidine (TMB).
8. as described in claim 1-7 PCNA (PCNA) antibody quantification kit preparation method, its feature
It is, with the antibody coated elisa plate purifying, make solid phase carrier；Toward being coated anti-proliferating cell nuclear antigen antibody (PCNA) antibody
ELISA Plate in sequentially add sample or standard items, biotinylated anti-PCNA antibody, horseradish peroxidase-labeled affine
Element.
9. as described in claim 1-7 PCNA (PCNA) antibody quantification kit using method, its feature
It is, comprise the steps：
(1) configuration standard liquid；
(2) it is loaded；
(3) discard liquid and dry, plus working solution；
(4) after incubating, discard in the hole liquid, dry；
(5) every hole adds Horseradish peroxidase-conjugated avidin working solution；
(6) after incubating, discard in the hole liquid, dry；
(7) sequentially every hole adds substrate solution；
(8) sequentially every hole adds stop bath；
(9) enzyme connection instrument measurement；Or further include：
(10) draw calibration curve.
10. the using method of PCNA (PCNA) antibody quantification kit as claimed in claim 9, its feature exists
In, the good all reagent of configuration in advance before step (1), when reagent or Sample Dilution, it is both needed to mix, has avoided during mixing as far as possible
Bubble；And/or,
In step (1), dissolve standard items with sample diluting liquid, concentration be made into 0.48ug/L, 0.24ug/L, 0.12ug/L,
0.06ug/L, 0.03ug/L, 0ug/L, wherein 0ug/L are the sample diluting liquid without standard items；And/or,
In step (2), set blank well, gauge orifice, testing sample hole respectively, blank well adds sample diluting liquid 100ul, remaining hole is respectively
Plus standard items or testing sample 100ul, it is loaded and sample is added on ELISA Plate bottom hole portion, gently rock mixing, ELISA Plate adds lid
Or overlay film, 37 DEG C are reacted 120 minutes；And/or,
In step (3), every hole adds biotin labelled antibodies working solution 100ul, 37 DEG C, 60 minutes；And/or,
In step (4), after incubating 60 minutes, discard in the hole liquid, dry, wash plate 3 times, soak 1-2 minute every time, 350ul/ is every
Hole, dries；And/or,
In step (5), every hole adds Horseradish peroxidase-conjugated avidin working solution 100ul, 37 DEG C, 60 minutes；And/or,
In step (6), after incubating 60 minutes, discard in the hole liquid, dry, wash plate 5 times, soak 1-2 minute every time, 350ul/ is every
Hole, dries；And/or,
In step (7), sequentially every hole adds substrate solution 90ul, 37 DEG C of lucifuge colour developings；And/or,
In step (8), sequentially every hole adds stop bath 50ul, terminating reaction；And/or,
In step (9), join, with enzyme, the optical density that instrument sequentially measures each hole in 450nm wavelength, after adding terminate liquid within 15 minutes
Detected；And/or,
In step (10), draw calibration curve, and the absorbance after example reaction is contrasted with calibration curve, determine that sample is dense
Degree, and it is multiplied by extension rate, both obtain raw sample concentration.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107894510A (en) * | 2017-12-13 | 2018-04-10 | 中国农业科学院生物技术研究所 | Quantitatively detect the enzyme linked immunological kit and its detection method of insect resistance protein Vip3Aa20 in transgenic corns |
| CN114397454A (en) * | 2021-12-03 | 2022-04-26 | 新疆医科大学第三附属医院 | Rapid human c-myc gene monitoring kit and use method thereof |
-
2016
- 2016-08-26 CN CN201610739959.6A patent/CN106405100A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 上海时代生物科技有限公司: "大鼠抗增殖细胞核抗原抗体（PCNA）酶联免疫分析（ELISA）说明书", 《中国教育装备采购网》 * |
| 党小军: "《临床免疫学检验技术》", 31 July 2014 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107894510A (en) * | 2017-12-13 | 2018-04-10 | 中国农业科学院生物技术研究所 | Quantitatively detect the enzyme linked immunological kit and its detection method of insect resistance protein Vip3Aa20 in transgenic corns |
| CN114397454A (en) * | 2021-12-03 | 2022-04-26 | 新疆医科大学第三附属医院 | Rapid human c-myc gene monitoring kit and use method thereof |
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