CN106264776B - Fatty chylosis method - Google Patents

Abstract

The invention relates to a fat chylolysis method, which comprises purifying and chylolysis extracted fat particles to obtain chylo-like fat, adopting step-by-step segmentation in the chylolysis process, and strictly controlling the step-by-step chylolysis of the fat particles diameter and times. The chytriation method of the present invention can realize fat transplantation in thin skin or sensitive parts, the skin is smooth after transplantation, the trauma is small, and the recovery speed is fast, which is suitable for non-structural transplantation such as improving skin quality and fine lines, and can be combined with structural Fat grafting together completes the improvement of skin quality.

Description

fat emulsion method

technical field

The invention relates to the field of fat shaping, in particular to a fat emulsion method for fine structure filling.

Background technique

Skin aging is a phenomenon of skin aging caused by natural or unnatural factors. After birth, the skin tissue becomes more and more developed, and its functions are gradually active. When it reaches a certain age, it will begin to degenerate, and this degeneration often proceeds slowly without people's awareness. The phenomenon of skin aging is mainly manifested in the decline of skin tissue. The thickness of the skin changes significantly with age. The human epidermis is the thickest at the age of 20, and then gradually becomes thinner. In old age, the granular layer can shrink to disappear, and the survival period of thorn cells is shortened. The dermis is thickest at the age of 30, and then gradually becomes thinner and atrophic. The subcutaneous fat is reduced, and the skin elasticity and tension are gradually lost due to changes in moisture, elastic fibers, and collagen fibers, which further lead to skin sagging and wrinkles.

Skin aging is a natural law of life and cannot be resisted, but people can delay skin aging through skin care and health care. The more commonly used method is the hyaluronic acid injection method. Hyaluronic acid exists in the dermal tissue of human skin in the form of gel, which is responsible for storing water, increasing skin volume, and making the skin look plump, plump, and elastic. However, the injected hyaluronic acid can be completely absorbed by the body, so Periodic injection is required, and its price is high and its maintenance time is short, so its audience is small.

Structural fat transplantation is an important method of modern fat plastic surgery. It is mostly used to improve the structural changes after soft tissue damage caused by disease, trauma or aging. Needles with a diameter of 0.84-1.19mm are often used to transplant fat particles to specific parts of the human body. Theory It has the possibility of improving skin aging, and the effect is longer than that of hyaluronic acid. However, for fat grafting on thin or sensitive parts of the skin, such as eyelids, lips, hands, etc., when the granular fat grafting is injected into the deep layer of the dermis, complications such as unevenness, fat cyst-like lesions, and calcified nodules are prone to occur , lack of good effect on skin texture improvement. Therefore, in order to make up for the deficiencies of structural fat grafting and improve skin texture, specific methods are used to reduce the size of transplanted fat particles (sometimes combined with structural fat grafting) to improve the effect of fat particles after filling, which is fat grafting. Improves the important process of skin aging.

Contents of the invention

In order to solve the above-mentioned technical problems, the inventors of the present invention have carried out intensive research and provided a fat chylolysis method, which includes purifying and chylolysis the extracted fat particles to obtain chylo-like fat. The way of fractionation, strictly control the particle size and times of fractionation, and strictly screen the fat particles to be chyle, use the chyle-like fat for filling, can achieve satisfactory filling effect and skin texture improvement effect, thus completing the present invention .

The object of the present invention is to provide a fat chylolysis method, which includes purifying and chylolysis the extracted fat particles to obtain chylotype fat, wherein, in the chylolysis process, the fat particles are processed in order from large to small The level-by-level segmentation method of the segmentation.

In a preferred embodiment, the purification includes rinsing of fat particles and removal of non-fat tissue. The rinsing solution is physiological saline at 0° C. to 4° C. The number of rinsing is 2 to 3 times, and each rinsing is 0.5 to 1 min.

In a preferred embodiment, after the fat particles are rinsed, the fat particles are separated from the rinsing liquid, and the separation method is selected from any one of filtration or centrifugation, preferably centrifugation, wherein the centrifugal speed is 1000r/min ~3000r/min, centrifugation time is 2min~3min.

In a preferred embodiment, after the fat particles and the rinsing solution are centrifuged, the fat particles located in the middle of the fat particle layer are collected for chylolysis. In the process of chylotylation, three kinds of dividers with different apertures 121 are used to divide the fat particles, and each of the three kinds of dividers is equipped with a dividing diaphragm 12.

The diameters of the three kinds of split holes 121 are respectively 0.8-1.2 mm, 0.6-0.8 mm and 0.4-0.6 mm,

The number of divisions of a divider with a diameter of 0.8 to 1.2 mm having a division hole 121 is 5 to 10 times,

The number of divisions of a divider with a diameter of 0.6 to 0.8 mm having a division hole 121 is 5 to 10 times,

The number of divisions of a divider having a division hole 121 with a diameter of 0.4 to 0.6 mm is 10 to 20 times.

According to a kind of fat emulsion method provided by the present invention, has following beneficial effect:

(1) The chytriation method in the present invention can realize the reasonable division of fat particles, combined with fat transplantation technology can realize fat transplantation in thin skin or sensitive parts, such as non-fat structural transplantation areas such as around the eyes, around the mouth, and hands, After transplantation, the skin is smooth, the trauma is small, the recovery speed is fast, and the skin elasticity can be significantly improved. It is suitable for non-structural transplantation that improves skin quality and fine lines;

(2) The present invention accurately defines the specific operation of the chylotylation method, and has guiding significance for chylotype fat transplantation.

Description of drawings

Fig. 1 shows a schematic structural view of a divider in a preferred embodiment of the present invention;

Fig. 2 shows a schematic structural view of a segmented diaphragm in a preferred embodiment of the present invention;

Fig. 3 shows a schematic structural view of the extraction needle in a preferred embodiment of the present invention.

Explanation of reference numbers:

11-separation cylinder;

12- split diaphragm;

121-division hole;

13-interface;

21 - needle tip;

22 - needle tube;

221 - pinhole;

222 - bending point;

23-connecting seat.

Detailed ways

The following describes the present invention in detail, and the features and advantages of the present invention will become more clear and definite along with these descriptions.

The word "exemplary" is used exclusively herein to mean "serving as an example, embodiment, or illustration." Any embodiment described herein as "exemplary" is not necessarily to be construed as superior or better than other embodiments. While various aspects of the embodiments are shown in drawings, the drawings are not necessarily drawn to scale unless specifically indicated.

According to the fat chylolysis method provided by the present invention, the method includes purifying and chylolysis the extracted fat particles to obtain chylotype fat.

In a preferred embodiment, the purification includes rinsing of fat particles and removal of non-fat tissue.

The rinsing liquid is physiological saline, and the fat particles are rinsed. After collecting the aspirated fat particles, they are immediately rinsed with physiological saline to wash away the blood carried out from the fat particles. The osmotic pressure of normal saline is the same as that of human plasma, which can avoid the rupture of fat cells, and after swelling and anesthesia, the osmotic pressure of fat cells changes (decreases), and the osmotic pressure of normal saline is higher than that of fat cells. Drainage of excess moisture.

The physiological saline used for rinsing is low-temperature physiological saline, and the temperature is 0°C to 4°C. At low temperature, the metabolism of substances in fat cells slows down, and the oxygen consumption decreases, which can maintain the activity of fat cells and increase the survival rate of fat cells during rinsing.

The number of rinsing with normal saline is 2-3 times, 0.5-1 min each time, and the rinsing of fat particles is completed in a very short time.

Fat particles are removed during rinsing, and non-fat tissue (mainly fibrous tissue) is removed manually. Since the fat particles are extracted from the patient's inner thigh or lower abdomen area with less fibrous tissue, the impurity removal work can be completed in a short time of rinsing, saving operation time. The patient is a natural person undergoing fat transplantation.

In a preferred embodiment, after rinsing with physiological saline, the fat particles in the upper layer of the physiological saline are collected to obtain a fat particle suspension, followed by solid-liquid separation of the fat particle suspension. The separation method is selected from any one of filtration or centrifugal separation, preferably centrifugal separation. During the centrifugation process, under the action of centrifugal force, more water in the fat cells due to swelling and anesthesia flows out further. After the centrifugation is completed, the size of the fat cells shrinks to about 1/2 of the size at the time of extraction.

The centrifugation process is at a rotating speed of 1000r/min to 3000r/min, centrifuging for 2min to 3min. The centrifugation speed and centrifugation time are strictly limited. If the centrifugation speed is too high or the centrifugation time is too long, it will easily lead to the rupture of fat cells. However, if the centrifugation speed is small, the cell debris brought by the extraction of fat particles cannot be removed to the maximum extent. At this rotational speed and time, the purity of adipocytes after centrifugation is significantly higher than that before centrifugation.

After the centrifugation is completed, the fat particle suspension is layered, the upper layer is a fat layer, the lower layer is a saline layer, and the middle is a fat particle layer. Fat granules located in the middle of the fat granule layer are used for chylolysis, preferably, the amount of selected fat granules is less than 1/3 of the total amount of fat granules. The upper part of the fat granule layer is close to the fat layer, and the upper fat granule is easy to adhere to the oil, while the lower part of the fat granule layer may have residual fibrous tissue. Impurities in both parts will affect the amount of fat implanted and affect the filling effect. The fat particles located in the middle 1/3 of the fat particle layer have uniform particle size, less fibrous tissue, and higher purity, which is the best choice for fat transplantation.

In a preferred embodiment, the chylosification device includes a fat divider and a pusher. As shown in FIG. 1 , the divider includes a dividing cylinder 11 and a dividing diaphragm 12 inside the dividing cylinder 11 . The split cylinder 11 is a hollow cylinder with openings at both ends that can accommodate fat particles, and is connected to the injector through the interface 13 arranged at the two open ends, that is, one divider is used in conjunction with two injectors. The outer surface of the interface 13 has threads for fixing the injector.

As shown in Figures 1 and 2, the split diaphragm 12 is clamped in the middle of the split cylinder 11, and the split diaphragm 12 is provided with a circular split hole 121, and the fat particles with a large particle size are divided into small fat particles through the split hole 121. Particle size fat particles. The number of divided diaphragms 12 on the divider is 1 to 3, preferably 1. The number of split holes 121 on the split diaphragm 12 is 1 to 3, when the number of split holes 121 is 1, the split hole 121 is located at the center of the split diaphragm 12; when the number of split holes 121 is 2, the two split holes 121 are arranged side by side on the center line of the split diaphragm 12; when the number of split holes 121 is 3, the three split holes 121 are arranged in a letter shape with the center line as the axis of symmetry. The shape of the circular segmented hole 121 conforms to the shape of the fat cell, and can maintain the integrity of the cell membrane of the fat cell to the greatest extent.

The injectors are located at both ends of the divider, and are used to suck fat particles to be divided and make them pass through the dividing holes 121 of the divider to complete the dividing process. The injector includes an injection tube and a piston rod located in the injection tube, and the fat particles are pushed to move by applying pressure to the piston rod. The injector can be a medical syringe.

The chylolysis of fat particles adopts the method of step-by-step segmentation. The step-by-step segmentation refers to the method of sequentially segmenting fat particles from large to small. method, rather than the commonly used method of dividing fat particles into the required particle size at one time. When dividing step by step, the pore size of the dividing hole 121 changes gradually from large to small, so that the particle size of the fat particles gradually changes. The pressure on the fat particles during the division is greatly reduced compared with the one-time division, and the fat cells are prevented from being squeezed. Severely deformed or broken during compression.

In a preferred embodiment, three types of dividers with different apertures 121 are used in the chylolysis process to divide the fat particles, and each of the three dividers is provided with a dividing membrane 12. The apertures of the split holes 121 are respectively 0.8 to 1.2mm, 0.6 to 0.8mm and 0.4 to 0.6mm, and the number of splits of a divider with a split hole 121 having a diameter of 0.8 to 1.2mm is 5 to 10 times. The number of divisions with the aperture of 0.6-0.8mm is 5-10 times, and the number of divisions with the aperture of 0.4-0.6mm with the division hole 121 is 10-20 times, and the light yellow chylus-like fat is obtained, wherein , the particle size of fat particles is less than 0.5mm.

In a further preferred embodiment, dividers with three kinds of dividing apertures are used, and the dividing holes 121 have diameters of 1.0 mm, 0.8 mm and 0.5 mm. When carrying out fat granule segmentation, adopt the splitter with splitting hole 121 aperture to be 1.0mm, the split frequency of fat particle is 5 times; Then adopt the splitter with split hole 121 aperture to be 0.8mm, the split times of fat particle is 10 times; Finally, a divider with a split hole 121 with a diameter of 0.5 mm is used, and the number of times of dividing the fat particles is 15.

The improvement of the skin effect of chylus fat on thin or sensitive parts is mainly obtained through the stimulating effect of chylus fat on the differentiation of fat cells in the body and tissue regeneration, as well as the filling effect produced by the implanted fat particles, so the fat The number of divisions of particles, especially the number of divisions when using the divider with the smallest dividing aperture, is crucial to the effect of fat implantation in patients. If the number of times is less than 10, the fat particles will not move smoothly in the injector, and the fat particles will be uneven and not delicate. At this time, injecting the patient will not only increase the difficulty of injection, but also make it difficult to grasp the smoothness of the filling area after injection, which will affect the operation. After effects; the number of divisions is more than 20 times, the fat particles move smoothly in the injector, and the fat particles are relatively uniform, but the fat particles are small and low-density due to multiple divisions, and the fluidity in the filling area is large, and it will be implanted in the patient soon. Absorbed or excreted by body tissues, its stimulating effect on fat cells in the body is limited. When the number of divisions was 10-20 times, the color of the fat particles changed from dark yellow to light yellow, the fat particles were uniform, and the density of the particles was moderate, which was suitable for transplantation.

In the present invention, the fat emulsion method further includes selecting a specific part of the patient to extract fat particles, and injecting the emulsion fat to the part to be filled.

In a preferred embodiment, the patient is subjected to local swelling and anesthesia before the fat extraction operation, and fat particles are extracted from a specific part of the patient.

Fat particles are extracted from the inner thigh or lower abdomen of the patient, not only because there is more fat in these parts, but more importantly, there is less fibrous tissue in these parts, which reduces the difficulty of post-processing, and the fat particles are full and easy to survive .

Before fat extraction, tumescent anesthesia is performed on the patient, and a large amount of anesthesia solution containing lidocaine and epinephrine is perfused subcutaneously. The local application of epinephrine causes local blood vessels to constrict, slows down drug absorption, and prolongs the anesthesia effect; fat and lidocaine It has affinity and can delay its absorption; at the same time, a large amount of swelling fluid is injected to cause edema of the subcutaneous tissue and its structure, and the tension of the local tissue increases, which can directly compress the capillaries and reduce the absorption of the drug, thus achieving local anesthesia and pain relief , Hemostasis and tissue separation effect. Due to the enlarged fat cell gap, it is not easy to cause a large area of fat cell damage during the fat extraction process.

In a preferred embodiment, the anesthetic solution uses physiological saline as a solvent, wherein the concentration of lidocaine is 0.025 (w/v)% to 0.1 (w/v)%, and the concentration of epinephrine is 0.5× 10 ^-7 (w/v)% to 1×10 ^-7 (w/v)%.

In a preferred embodiment, the extracted fat particles are extracted by using an extraction needle. As shown in Figure 3, the extraction needle includes a needle point 21, a needle tube 22 and a connecting seat 23 connected in sequence, the needle point 21 is not perforated, and is used to guide the movement of the needle tube 22; the needle tube 22 is used to transport and extract to Fat particles, the end connected to the needle tip 21 is provided with a plurality of needle holes 221 distributed in a spiral shape; the connecting seat 23 is used to connect the needle tube 22 and the negative pressure device. The negative pressure device uses negative pressure to draw fat particles out of the body through the extraction needle, and is a professional medical equipment, such as a syringe.

The pinholes of the existing fat extraction needles are arranged in the shape of "pin", which may easily cause problems such as uneven fat extraction, uneven fat extraction area, and uneven cohesive liposuction causing blockage in the needle tube. In the present invention, the helically distributed pinholes 221 make the extraction needle form a columnar fat extraction in the fat layer, and the columnar fat extraction can extract fat cells to the greatest extent per unit area, and the fat extraction is coherent and uniform, avoiding reciprocating multiple extractions, and not The problem of needle tube 22 blockage during the operation will occur, which has the characteristics of less tissue damage and less color in the eye of the needle. At the same time, the anesthesia time and anesthesia drugs are reduced, the accumulation and metabolism of anesthetic drugs in the body are reduced, and the safety of the operation is increased.

In a further preferred embodiment, the pinhole 221 is circular or elliptical. Since mature fat cells are circular or elliptical, the shape of the pinhole 221 conforms to the shape of the fat cell and can maintain the fat cell membrane to the greatest extent. integrity. According to the extraction needles of different lengths and diameters, the number of pinholes 221 is 3 to 5, preferably 3, which can better maintain the negative pressure in the extraction needle while ensuring the maximum amount of fat extraction.

In a further preferred embodiment, according to the characteristics that the negative pressure at the proximal end of the needle tip 21 is small and the negative pressure at the distal end of the needle tip 21 is large, in the spiral direction from the needle tip 21 to the connecting seat 23, the diameter of the needle hole 221 Distributed from large to small, this pore size design ensures uniform pressure during the extraction process and protects the consistent shape of fat cells. Preferably, the needle tube 22 of the extraction needle is bent in a V shape at the end close to the connecting seat 23, and the bending angle is 120°-135°. More preferably, the distance between the bending point 222 and the connecting seat 23 is 1/5-1 of the length of the needle tube 22. /7. In a specific fat extraction site, such as the buttocks, the curved extraction needle is more convenient for the operator to use than the non-curved extraction needle. It can easily complete 3 faces and a total of 360° fat extraction, which conforms to the physiological curvature of the human body. The bending point 222 is the point at which the pointer tube 22 begins to bend.

When chylotype fat is implanted into the deep dermis of patients, multi-point, multi-level, and small-scale injections are used to ensure the survival rate of fat cells and avoid problems such as unevenness, fat cyst-like changes, and central necrosis of fat granules.

Chyloid fat injection is equivalent to the process of autologous tissue transplantation, which is in line with medical norms. It can be used to improve skin quality, improve fine lines and other non-structural transplant rejuvenation fields, and may become the standard for non-structural transplant rejuvenation in the future. In fat surgery, chylous fat grafting (unstructured fat grafting) is often used together with structural fat grafting (granule cell transplantation). For example, when deep wrinkles are improved, larger granule cells need to be implanted deep in the skin. Cooperate with the superficial chylous fat to complete it together.

Example

Example 1

(1) Preparation method of chylous fat

1. Select the patient’s inner thigh area, use a special liposuction needle for liposuction, and inject a small dose of low concentration (0.05 (w/v)% lidocaine + 10 ^-7 (w/v)% epinephrine) before surgery Local tumescent anesthesia;

2. After collecting the suctioned fat particles, rinse twice with 4°C normal saline for 1 min/time, let stand for 0.5 min, collect the fat particles in the upper layer, and centrifuge at 3000r/min for 2 min for solid-liquid separation, and separate the upper part of the suspended fat Suction out, the water from the lower end is discharged;

3. Select the fat granules in the middle 1/3 of the fat granule layer, extract the fat granules with a 1mL spiral syringe, and use 1.0mm, 0.8mm and 0.5mm dividers with three apertures to divide the fat granules. The number of diaphragms is 1, and the number of split holes is 3, 2 and 1 in sequence. First, use a divider with a 1.0mm aperture to divide 5 times; then use a divider with a 0.8mm aperture to divide 10 times; finally, use a divider with a 0.5mm aperture to divide 15 times to obtain chylous fat.

(2) Effect of injection of chylous fat

Patient: Female, 10 people, 47-55 years old, with obvious forehead lines and crow's feet (aging fine lines around the eyes, pigmentation around the eyes), fat chylotherapy and structural fat grafting were used to fill the wrinkled areas.

The extracted chyle fat was injected deep into the dermis with a 27G needle (inner diameter 0.21 mm, outer diameter 0.41 mm) until the skin color changed to yellow. This discoloration usually goes away a few hours after the injection.

After 6 months of follow-up, after the chylotype fat and structural fat transplantation, the forehead lines improved significantly, the fine lines around the eyes disappeared, the fat cell transplantation effect was satisfactory, the pigmentation improved and the local skin elasticity increased. Observation found that chylotype fat has obvious skin rejuvenation effect on the patient's face, and the specific results are shown in Table 1.

Table 1 Comparison of preoperative and postoperative results

Note: 1. Conspicuousness of forehead lines or crow’s feet: Level 1: No visible creases; only continuous skin lines; Level 2: Visible crease lines, slight indentations, and small facial creases; Level 3: Comparative Deep folds, facial folds are clear, visible under normal circumstances; grade 4: deep folds; facial folds are obvious; visible folds less than 2 mm when stretched; grade 5: extremely deep folds, There are clearly visible creases of 2-4mm when stretched.

2. Flatness (caused by surgery): Level 1: No obvious protrusion or depression; Level 2: Average 0.5mm protrusion at the surgical site; Level 3: Average 1mm protrusion at the surgical site; Level 4: Surgical site with The average protrusion is greater than 1-2mm; grade 5: the surgical site has an average protrusion greater than 2mm.

3. Pigmentation around the eyes: grade 1: no obvious pigmentation; grade 2: light, slightly visible pigmentation; grade 3: obvious pigmentation; grade 4: obvious pigmentation, dark color.

4. Skin elasticity: Level 1: Good elasticity; Level 2: Good elasticity; Level 3: No obvious elasticity; Level 4: Poor elasticity.

Example 2

(1) Preparation method of chylous fat

1, step 1 is the same as embodiment 1;

2. After collecting the aspirated fat particles, rinse with 4°C normal saline for 0.5 min/time for 3 times, let stand for 0.5 min, collect the fat particles in the upper layer, centrifuge at 2000r/min for 2 min for solid-liquid separation, and suspend the upper part The grease is sucked out, and the water at the lower end is discharged;

3. Select the fat granules in the middle 1/3 of the fat granule layer, extract the fat granules with a 1mL spiral syringe, and use 1.0mm, 0.8mm and 0.5mm dividers with three apertures to divide the fat granules. The number of diaphragms is 1, and the number of split holes is 3, 2 and 1 in sequence. First, use a divider with a 1.0mm aperture to divide 5 times; then use a divider with a 0.8mm aperture to divide 10 times; finally, use a divider with a 0.5mm aperture to divide 10 times to obtain chylous fat for later use.

(2) Effect of injection of chylous fat

Patient: Female, 10 people, 52-61 years old, with obvious neck lines and mouth corner lines. Fat chylotherapy and structural fat grafting were used to fill the wrinkled area.

The extracted chylofat was injected deep into the dermis with a 27G needle until the skin color changed to yellow.

After 6 months of follow-up, after the transplantation of chylous fat and structural fat, the neck lines were significantly improved, the mouth corner lines disappeared and the skin was smooth and smooth, which significantly improved the quality of the patient's skin and improved the skin color. Table 2 shows.

Table 2 Comparison of preoperative and postoperative results

Note: Judgment criteria for wrinkle degree, smoothness and skin elasticity are the same as Table 1.

Example 3

(1) Preparation method of chylous fat

Steps 1-3 are the same as in Example 1.

(2) Effect of injection of chylous fat

Patient: Female, 10 people, 26-32 years old, with obvious scar after caesarean section, fat chylotherapy was used to fill the scar area.

The extracted chylofat was injected deep into the dermis with a 27G needle.

After 6 months of follow-up, the elasticity of the scar area was significantly improved after chylos fat transplantation, the color difference between the color and the surrounding skin was reduced, and the quality of the skin in the scar area was significantly improved. The specific results are shown in Table 3.

Table 3 Comparison of preoperative and postoperative results

Note: Judgment criteria for smoothness, skin elasticity and pigmentation are the same as Table 1.

Comparative example 1

(1) Preparation method of chylous fat

1, step 1 is the same as embodiment 1;

2. After collecting the suctioned fat particles, rinse them twice with normal temperature saline for 1 min/time, let stand for 0.5 min, collect the fat particles in the upper layer, and centrifuge at 600r/min for 2 min for solid-liquid separation, and suck out the upper part of the suspended fat , the water from the lower end is discharged;

3. Select all the fat particles in the fat particle layer, extract the fat particles with a 1mL spiral syringe, and directly use a splitter with a split aperture of 0.5mm to split the fat particles. The number of split diaphragms in the splitter is 1, and the number of split holes is in order for 3 pcs. The divider divides 30 times to obtain chylous fat. During the process of chylotylation, the resistance of fat passing through the segmentation hole increases, which may cause a large amount of crush damage to the adipocytes, and the process of making chyle-like fat is prone to stagnation.

(2) Effect of injection of chylous fat

Patient: Female, 10 people, 42-50 years old, with obvious forehead lines and crow's feet. Fat chylotherapy and structural fat grafting were used to fill the wrinkled area.

The extracted chylofat was injected deep into the dermis with a 27G needle until the skin color changed to yellow.

After 6 months of follow-up, after the chylous fat and structural fat transplantation, the forehead lines and crow's feet are still more obvious, and have not been significantly improved, and the skin quality and skin color have no major changes. The specific results are shown in the table 4.

Table 4 Comparison of preoperative and postoperative results

Note: Judgment criteria for wrinkle degree, smoothness, skin elasticity and pigmentation are the same as Table 1.

Comparative example 2

(1) Preparation method of chylous fat

1, step 1 is the same as embodiment 1;

2. After collecting the suctioned fat particles, rinse twice with 4°C normal saline for 1 min/time, let stand for 0.5 min, collect the fat particles in the upper layer, and centrifuge at 3000r/min for 2 min for solid-liquid separation, and separate the upper part of the suspended fat Suction out, the water from the lower end is discharged;

3. Select all the fat granules in the fat granule layer, extract the fat granules with a 1mL spiral syringe, and use 1.0mm, 0.8mm, and 0.5mm dividers with three apertures to divide the fat granules. Among them, the number of divided diaphragms in each divider is 1, and the number of split holes is 3, 2 and 1 in turn. First, use a divider with a 1.0mm aperture to divide 5 times; then use a divider with a 0.8mm aperture to divide 5 times; finally, use a divider with a 0.5mm aperture to divide 5 times to obtain chylous fat for later use.

(2) Effect of injection of chylous fat

Patient: Female, 10 people, 50-63 years old, with obvious forehead lines and crow's feet. Fat chylotherapy and structural fat grafting were used to fill the wrinkled area.

The extracted chylofat was injected deep into the dermis with a 27G needle until the skin color changed to yellow.

After 6 months of follow-up, after the chylotype fat and structural fat transplantation, the skin smoothness was occasionally uneven, and the skin elasticity was limited. The specific results are shown in Table 5.

Table 5 Comparison of preoperative and postoperative results

Note: Judgment criteria for wrinkle degree, smoothness, skin elasticity and pigmentation are the same as Table 1.

The present invention has been described in detail above in conjunction with specific implementations and/or exemplary examples, but these descriptions should not be construed as limiting the present invention. Those skilled in the art understand that without departing from the spirit and scope of the present invention, various equivalent replacements, modifications or improvements can be made to the technical solutions and implementations of the present invention, all of which fall within the scope of the present invention. The protection scope of the present invention shall be determined by the appended claims.

Claims (9)

1. a fat chylolysis method, is characterized in that, described method comprises that the fat granule that extracts is purified, chylolysis, obtains chylotype fat, Among them, the step-by-step segmentation method of sequentially segmenting fat particles from large to small is adopted in the process of chylolysis; The purification includes rinsing of fat particles and removal of non-fat tissue, and the rinsing solution is 0°C-4°C physiological saline; After the fat particles are rinsed, the fat particles are separated from the rinsing solution. The separation method is centrifugation, the centrifugal speed is 1000r/min-3000r/min, and the centrifugation time is 2min-3min; The chytriation device in the chytriation process includes a fat divider and a bolus; In the process of chylotylation, three kinds of dividers with different pore diameters are used to divide the fat particles. Each of the three kinds of dividers is equipped with a dividing diaphragm (12), and the dividing diaphragm (12) is provided with a circular split hole, The diameters of three different circular split holes are 0.8-1.2mm, 0.6-0.8mm and 0.4-0.6mm respectively. The number of divisions of a divider with a division hole with a diameter of 0.8 to 1.2 mm is 5 to 10 times, The number of divisions of a divider with a division hole with a diameter of 0.6 to 0.8 mm is 5 to 10 times, The number of divisions of a divider with a division hole with a diameter of 0.4 to 0.6 mm is 10 to 20 times; The extracted fat particles are extracted by using an extraction needle, and the extraction needle includes a needle point (21), a needle tube (22) and a connecting seat (23) connected in sequence, and the needle point (21) is used to guide the needle tube ( 22), the needle tube (22) is used to transport the extracted fat particles, and the end connected to the needle point (21) is provided with a plurality of pinholes (221) distributed in a spiral shape, and the connecting seat (23 ) is used to connect the needle tube (22) and the negative pressure device, In the helical direction along the needle tip (21) to the connection seat (23), the apertures of the needle holes (221) are distributed from large to small. 2. The method of claim 1, wherein, The number of rinsing times for the fat particles is 2 to 3 times, each time for 0.5 to 1 min. 3. The method according to claim 1, characterized in that, after the fat particles and the rinsing solution are centrifuged, the fat particles located in the middle of the fat particle layer are selected for chylolysis operation. 4. The method according to claim 3, characterized in that the amount of selected fat particles is less than 1/3 of the total amount of fat particles. 5. The method according to one of claims 1 to 4, characterized in that The divider comprises a dividing cylinder (11) and a dividing diaphragm (12) located in the dividing cylinder (11). The dividing cylinder (11) is a hollow cylinder which is open at both ends and can accommodate fat particles. The interface (13) at the two open ends is connected with the injector, and the outer surface of the interface (13) has threads to fix the injector, and the split diaphragm (12) is clamped in the middle of the split cylinder (11). Fat particles with particle size are divided into small-size fat particles through the partition hole; The injectors are located at both ends of the divider, and are used to absorb the fat particles to be divided, and make them pass through the dividing holes of the divider to implement the chylolysis process. 6. The method according to claim 1, wherein the fat particles are extracted from the patient's inner thigh or lower abdomen area. 7. The method according to claim 1, characterized in that the pinhole (221) of the extraction needle is circular or oval. 8. The method according to claim 1, characterized in that the needle tube (22) of the extraction needle is bent in a V shape at an end close to the connecting seat (23), and the bending angle is 120°-135°. 9. The method according to claim 8, characterized in that, the distance between the bending point (222) and the connecting seat (23) is 1/5-1/7 of the length of the needle tube (22).