CN106259346B - A kind of nematicidal composition of containing fluopyram and Tea Saponin - Google Patents

Abstract

The present invention relates to a kind of pesticide composition that comprises fluopyram and tea saponin, and the active ingredient of described pesticide composition comprises fluopyram and tea saponin, wherein, the weight ratio of fluopyram and tea saponin is 1:50‑40:1, preferably 1:10‑20:1. The two active components of the composition of the present invention cooperate with each other, can improve the control effect, reduce the use of pesticides, and are safe to the environment, and are worthy of popularization and application.

Description

A nematocidal composition containing fluopyram and tea saponin

technical field

The invention relates to a synergistic nematicide composition, the active ingredients of which include fluopyram and tea saponin.

Background technique

Soil-borne diseases refer to plant diseases caused by the infection of soil-borne pathogens, which belong to the category of root diseases. Infection pathogens include fungi, bacteria, actinomycetes, nematodes, etc. Among them, fungi are the main ones, which are divided into non-obligate parasitism and obligate parasitism Two categories. Non-obligate parasitism is ectophytic root-infecting fungi, such as seedling rot and damping-off caused by Pythium, and seedling blight caused by mycobacterium sclerotia. Obligate parasitism is a pathogenic fungus of plant microtubules, typically wilting and dying caused by Fusarium oxysporum and Verticillium chrysogenum. The severity of root disease is affected by the composition and concentration of root exudates. Therefore, inhibiting the activity of pathogenic bacteria in the rhizosphere system becomes the basis for protecting the root system and preventing and controlling soil-borne diseases. However, attention must be paid to the restrictive effect of soil physical and chemical factors on the relationship between plants, soil microorganisms and root pathogens.

Tea saponin is a plant insecticide extracted from tea seed cake. It belongs to pentacyclic triterpenoids. Its pure product is a colorless columnar crystal with a molecular formula of C ₅₇ H ₉₀ O ₂₆ , a molecular weight of 1191.31, and a melting point of 223-224 ℃, easily soluble in water, alcohols, organic acids and other organic solvents, insoluble in aliphatic hydrocarbons and benzene solvents, this compound has multiple functions of killing insects, bacteria, mites and reducing surface tension, which is different from chemical pesticides It has a significant synergistic effect on chemical pesticides by improving the wetting, spreading, emulsifying and dispersing properties of the liquid, and has a unique killing effect on harmful organisms such as snails that are difficult to be effective with chemical pesticides. It is a typical non-toxic Variety of public nuisance multifunctional plant insecticides. However, the drug has poor quick-acting properties and a large dosage, so it is generally not used alone.

Fluopyram is a pyridylethylbenzamide fungicide with a wide bactericidal spectrum. It is used to control gray mold, powdery mildew, sclerotinia and Diseases caused by Candida spp. Fluopyram used alone or in combination with other fungicides has very good efficacy at low doses, and is very suitable for disease resistance management. Fluopyram acts on complex II or succinate-CoQ reductase in the mitochondrial respiratory electron transport chain, and it was the first nematicide to act on this target.

The mixing of different pesticide varieties is a common method to prevent and control agricultural resistant diseases, insects and weeds. Through the screening of pesticide formulas, screening out reasonable formulas can effectively improve the actual control effect, reduce the dosage of pesticides, reduce costs, and delay the emergence of disease resistance. It is an important means of comprehensive management of diseases, insects, and weeds. The inventors of the present application conducted in-depth research and experiments on the compounding of fluopyram and tea saponin, and found that the compounding of fluopyram and tea saponin can significantly increase the growth rate of nematodes within a certain range of mixing ratios. The effect can effectively prevent and control crop nematodes, and the reduction of dosage meets the demand of "zero growth of pesticides", and the pesticide composition containing fluopyram also has part-time effects on various other diseases. After further research, the present invention has been completed. At present, there is no relevant report on the compounding of fluopyram and tea saponin.

Contents of the invention

The object of the present invention is to address the deficiencies of the prior art and provide a pesticide composition that can simultaneously control crop root-knot nematodes and seedling pests. To achieve the above object, the present invention adopts the following technical solutions:

A pesticide composition, characterized in that the active ingredient comprises fluopyram and tea saponin, wherein the weight ratio of fluopyram and tea saponin is 1:50-40:1, preferably 1:10- 20:1.

Furthermore, the pesticide composition of the present invention can be prepared into any dosage form allowed in the processing of pesticide formulations by adding pesticide auxiliary components through conventional methods, preferably wettable powder, granule, granule, suspension concentrate and the like.

Further, the pesticide composition also includes other agricultural active ingredients, such as insecticides, fungicides, herbicides, fertilizers, and safeners.

Further, the application method of the pesticide composition of the present invention adopts one of spreading, furrow application, hole application, etc., and the application frequency and application amount vary with weather conditions and nematode damage, and can be achieved by applying an appropriate dose. Prevention purpose.

Furthermore, the pesticide composition of the present invention is suitable for controlling underground pests of crops, especially root-knot nematodes.

Compared with prior art, the beneficial effect of the present invention is:

(1) The nematicidal composition of the present invention has a synergistic effect on the control of nematodes, reduces the amount of pesticides used, increases the control effect, and is beneficial to save drug costs and environmental pollution;

(2) The nematicide composition of the present invention has the advantages of easy process control and stable product quality, can be produced continuously, and is suitable for industrialization.

Detailed ways

In order to make the object, technical solution and advantages of the present invention more clear, the present invention is illustrated with the following specific examples, but the present invention is by no means limited to these examples. The following descriptions are only preferred embodiments of the present invention, which are only used to explain the present invention, but should not be construed as limiting the patent scope of the present invention. It should be pointed out that any modification, equivalent replacement or improvement made within the spirit and principle of the present invention shall be included in the protection scope of the present invention. Claims prevail.

One, preparation embodiment

1. Example 1: 31% fluopyram·tea saponin suspension concentrate (30:1)

Fluopyram 30%

Tea Saponin 1%

Fatty alcohol polyoxyethylene ether 5%

NNO 3%

Magnesium Aluminum Silicate 1.4%

Xanthan Gum 0.2%

Ethylene glycol 1%

Deionized water balance

2. Example 2: 22% fluopyram·tea saponin wettable powder (10:1)

Fluopyram 20%

Tea Saponin 2%

Calcium dodecylbenzenesulfonate 2%

Sodium lignosulfonate 3%

Silica 7%

Diatomaceous earth balance

3. Example 3: 30% fluopyram·tea saponin wettable powder (1:1)

Fluopyram 15%

Tea Saponin 15%

Calcium dodecylbenzenesulfonate 3%

Sodium lignosulfonate 4%

Silica 7%

Diatomaceous earth balance

4. Example 4: 30% fluopyram·tea saponin suspension concentrate (17:13)

Fluopyram 17%

Tea Saponin 13%

Fatty alcohol polyoxyethylene ether 5%

NNO 3%

Magnesium Aluminum Silicate 1.4%

Xanthan Gum 0.2%

Ethylene glycol 1%

Deionized water balance

2. Indoor toxicity test

Test name: Indoor toxicity determination of fluopyram and tea saponin against cucumber root-knot nematode

Test method: Take the cucumber root system that produces a large number of root knots, rinse it gently with tap water, gently pick out the milky white oocysts on the diseased root with a dissecting needle, put it into a small petri dish with a diameter of 6 cm, add a small amount of sterile water, and dry it at 25 Incubate in an incubator at ℃ for 3-4 days, collect the 2nd instar larvae, and add sterile water to make it into a suspension with a certain concentration (about 500 pieces/ml) for later use.

Set up different concentration gradient treatments, use sterile water as the blank control, take 96-well cell culture plates, add 150mL of different concentrations of prepared medicines to each well, and then add an equal volume of nematode suspension, keep moisture and culture at 25 °C, respectively At different times after treatment, the number of survival and death of the second instar of M. incognita was checked. The nematodes were stiff and motionless as dead worms, and the line filling was bent and wriggling as live worms. Mortality and adjusted mortality were calculated.

Adjusted mortality rates for each treatment were calculated from the survey data. And refer to NY/T1154.1-2006 and use Sun Yunpei method to calculate the co-toxicity coefficient (CTC value) of the mixture. If the control mortality rate is <5%, no adjustment is made; if the control mortality rate is between 5-20%, adjustment is made; if the control mortality rate is >20%, the test needs to be repeated. The logarithm value of the drug concentration (mg/L) was used as the independent variable x, and the probability of corrected mortality rate was used as the dependent variable y to establish a regression equation for toxicity, and use DPS software to calculate the LC50, LC50, and The 95% confidence limit and the co-toxicity coefficient of the mixture were used to compare the synergy. According to the NY/T11547.7-2006 classification standard of combined action of insecticides: co-toxicity coefficient (CTC value) ≥ 120 means synergistic effect; co-toxicity coefficient (CTC value) ≤ 80 means antagonism; 80 < co-toxicity coefficient ( CTC value) <120 is an additive effect. Table 1 shows the combined effects of fluopyram and tea saponin on root-knot nematode cucumber.

The results of indoor toxicity test showed that the co-toxicity coefficients of fluopyram and tea saponin in the range of 1:50-40:1 for the control of cucumber root-knot nematode were all above 120, showing a synergistic effect. Wherein, when the weight ratio of fluopyram and tea saponin in the composition is 1:10-20:1, the co-toxicity coefficients are all higher than 160, and the synergistic effect is more significant.

3. Field drug efficacy test

In order to better verify the field application effect of the pesticide composition of the present invention, the pesticide composition of the embodiment of the present invention 1-4 is used to carry out the field efficacy test of controlling cucumber root-knot nematode disease, and each treatment is repeated three times, and each plot is repeated. Arranged in random blocks, the plot area is 20 m ² . Before sowing, apply pesticides and mix lightly, and sow randomly. The number of root-knot nematodes was investigated 20 and 40 days after the drug treatment. Adopt the diagonal sampling method, sample 3 plants at each point, investigate the disease index and the record standard of the incidence of root-knot nematode damage, and divide the disease into five grades according to the number of root-knot nematodes:

Level 0 no root knot;

Grade 1 root knot accounts for 1-25% of the whole root system;

Grade 2 root knot accounts for 26-50% of the whole root system;

Grade 3 root knot accounts for 51-75% of the whole root system

Grade 4 root knot accounts for 76-100% of the whole root system

Calculate the control effect of cucumber root-knot nematode according to formula (5) and formula (6):

.

Field efficacy test results show that the composition of fluopyram and tea saponin exhibits excellent persistent and quick-acting effects on cucumber root-knot nematodes. 20 days after the drug, the control effects were all higher than 80%, obviously due to the single-dose control. In addition, within the range of application, the cucumbers treated with each agent grew well and showed no phytotoxicity. In summary, the pesticide composition of the present invention is worthy of popularization and application in production.

Claims (3)

1. a kind of composition pesticide, which is characterized in that active constituent is fluopyram and Tea Saponin, wherein fluopyram Weight ratio with Tea Saponin is 1:1-20:1.

2. composition pesticide according to claim 1, which is characterized in that the composition pesticide is aided with Pesticide formulation In auxiliary element be prepared into any one dosage form agriculturally allowed.

3. the purposes of composition pesticide according to claim 1 or 2, which is characterized in that for preventing and kill off root-knot nematode.