CN106077075A - A kind of biological reinforced restorative procedure of gas station contaminated soil - Google Patents
A kind of biological reinforced restorative procedure of gas station contaminated soil Download PDFInfo
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- CN106077075A CN106077075A CN201610426581.4A CN201610426581A CN106077075A CN 106077075 A CN106077075 A CN 106077075A CN 201610426581 A CN201610426581 A CN 201610426581A CN 106077075 A CN106077075 A CN 106077075A
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- contaminated soil
- soil
- gas station
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- mixed vaccine
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Processing Of Solid Wastes (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
Abstract
The invention discloses the biological reinforced restorative procedure of a kind of gas station contaminated soil, comprise the steps: Pseudomonas aeruginosa and surfactant hydrocarbon degradation bacteria 1:1 by volume are configured to mixed vaccine；Mixed vaccine is added contaminated soil, and mixed vaccine and contaminated soil are puddled uniformly, obtain the contaminated soil containing mixed vaccine；Add, to contaminated soil, the nutrient substance that mixed vaccine growth and breeding needs, and add biosurfactant to contaminated soil；And in contaminated soil, it is passed through oxygen.Beneficial effects of the present invention: technique is simple, low cost, workload are little, and the product used by reparation is biological product, applies effective, will not produce secondary pollution to environment, is a kind of effective biological reinforced restorative procedure.
Description
Technical field
The invention belongs to field of environmental improvement, be specifically related to the biological reinforced restorative procedure of a kind of gas station contaminated soil.
Background technology
Gas station is oil storage tank and the place of pipeline concentration, and during gas station's operation, gasoline, the evaporating, emitting, dripping or leaking of liquid or gas of diesel oil are
Gas station and the potential pollution source of periphery place soil and groundwater, contamination index of gas station includes total petroleum hydrocarbon (TPH, C6~C9、
C10~C40), BTEX(benzene, toluene, ethylbenzene, dimethylbenzene), MTBE(methyl tertiary butyl ether(MTBE)), gas station of China place soil and underground
Water pollution control work is still in space state, and contaminated site repair is very urgent.
Summary of the invention
For the above-mentioned technical problem in correlation technique, the present invention proposes biological reinforced the repairing of a kind of gas station contaminated soil
Compound recipe method, in order to solve the problems referred to above.
For realizing above-mentioned technical purpose, the technical scheme is that and be achieved in that:
The biological reinforced restorative procedure of a kind of gas station contaminated soil, comprises the steps:
S1, Pseudomonas aeruginosa and surfactant hydrocarbon degradation bacteria 1:1 by volume are configured to mixed vaccine；
S2, mixed vaccine is added contaminated soil, and mixed vaccine and contaminated soil are puddled uniformly, obtain the pollution containing mixed vaccine
Soil；
S3, the nutrient substance needed to contaminated soil addition mixed vaccine growth and breeding, and it is alive to add biological surface to contaminated soil
Property agent；And
S4, in contaminated soil, it is passed through oxygen.
Further, after mixed vaccine first adds soil and puddles uniformly with soil, by nutrient substance and biological surface
Activating agent 1:1 by volume uniformly sprays to soil, sprays once every 15 days later, and sprinkling ratio presses nutrient substance
With biosurfactant volume ratio 1:1, the addition of the mixed solution of described nutrient substance and biosurfactant is every
Cubic meter contaminated soil adds 5-10L.
Further, the water content control of the contaminated soil containing mixed vaccine is at 30-45%, when water content is less than 30-45%
Time, carry out moisturizing of spraying water.
Further, in S1, the cell concentration of described Pseudomonas aeruginosa and surfactant hydrocarbon degradation bacteria is all higher than 108Individual/g soil
Earth.
Further, in S2, described mixed vaccine addition is that every cubic metre of contaminated soil adds 10-20L.
Further, described Pseudomonas aeruginosa uses aerobic liquid fermentation method, comprises the steps:
S101, will spread cultivation after seed liquor be forwarded to the fermentation tank culture medium after sterilizing according to the inoculum concentration that volume ratio is 3%-5%
In, and regulate the pH value of fermentation medium to 6-7, keep temperature 30.5-31.5 DEG C, tank pressure 0.09-0.1MPa；
S102, being aerated, stir fermentation, wherein, ventilation is 30m3/ h, rotating speed is 120rpm；
S103, cultivation 18h-24h, function bacterium quantity >=10 of the microbial inoculum obtained after liquid fermentation8；
Wherein, wherein, the culture medium of liquid fermentation includes the composition of following percentage by weight: the peptone of 1%, the yeast of 0.5%
Cream, the water of the NaCl of 1% and 97.5%.
Further, described surfactant hydrocarbon degradation bacteria uses aerobic liquid fermentation method, comprises the steps:
S111, will spread cultivation after seed liquor be forwarded to the fermentation tank culture medium after sterilizing according to the inoculum concentration that volume ratio is 3%-5%
In, and regulate the pH value of fermentation medium to 6-7, keep temperature 30.5-31.5 DEG C, tank pressure 0.09-0.1MPa；
S112, being aerated, stir fermentation, wherein, ventilation is 30m3/ h, rotating speed is 120rpm；
S113, cultivation about 18h, function bacterium quantity >=10 of the microbial inoculum obtained after liquid fermentation8；
Wherein, the culture medium of liquid fermentation includes the composition of following percentage by weight: the peptone of 1%, the yeast extract of 0.5%, 1%
The water of NaCl and 97.5%.
Further, described nutrient substance includes the carbamide of the composition of following percentage by weight: 5-10%, 1-5%
KH2PO4, the K of 1-5%2HPO4, the yeast extract of 0.5-1%, the trace element of 0.1-0.5%, remaining be water.
Further, described biosurfactant is rhamnolipid biological surface activator, described Bio-surface active
In agent, the concentration of main constituent is 0.5-2%, and remaining is water.
Further, described oxygen is passed through the soil containing mixed vaccine by blower fan, gas distribution pipeline；Described gas distribution pipeline includes
Passage aperture on main line and bye-pass, main line and bye-pass is 2-5mm, and the distance between adjacent vent holes is 4-
6cm, the ventilation of every cubic metre of soil is 3-5m3/h。
Beneficial effects of the present invention: technique is simple, low cost, workload are little, and the product used by reparation is biological product,
Apply effective, environment will not be produced secondary pollution, be that a kind of gas station's contaminated soil that is suitable for is administered the most biological reinforced
Restorative procedure.
Detailed description of the invention
Technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described enforcement
Example is only a part of embodiment of the present invention rather than whole embodiments.Based on the embodiment in the present invention, this area is common
The every other embodiment that technical staff is obtained, broadly falls into the scope of protection of the invention.
The biological reinforced restorative procedure of a kind of gas station of the present invention contaminated soil, comprises the steps:
S1, Pseudomonas aeruginosa and surfactant hydrocarbon degradation bacteria 1:1 by volume are configured to mixed vaccine；Wherein, Pseudomonas aeruginosa and hydrocarbon
The cell concentration of degradation bacteria is all higher than 108Individual/g soil；
S2, mixed vaccine is added contaminated soil, and mixed vaccine and contaminated soil are puddled uniformly, obtain the pollution containing mixed vaccine
Soil；In step s 2, described mixed vaccine addition is that every cubic metre of contaminated soil adds 10-20L；
S3, the nutrient substance needed to contaminated soil addition mixed vaccine growth and breeding, and it is alive to add biological surface to contaminated soil
Property agent；
Wherein, the function bacterium of present invention growth and breeding in contaminated site soil and groundwater needs suitable nutrient substance, raw
Thing method repair process adds biosurfactant enhancing degradation process, to improve speed and the degradation effect of degradation process.Raw
Thing surfactant is conducive to the emulsifying of the hydrocarbons pollutant in soil, it is possible to accelerate the function bacterium utilization to hydrocarbons pollutant,
To improve speed and the degradation effect of degradation process
S4, in contaminated soil, it is passed through oxygen.
After mixed vaccine first adds soil and puddles uniformly with soil, nutrient substance and biosurfactant are pressed body
Long-pending uniformly spraying to soil than 1:1, sprayed once every 15 days later, and sprinkling ratio presses nutrient substance and biological table
Face activating agent volume ratio 1:1, the addition of the mixed solution of described nutrient substance and biosurfactant is every cubic metre of dirt
Dye soil adds 5-10L.
In order to ensure the optimum humidity environment that microbial growth needs, the water content of the contaminated soil containing mixed vaccine
Control at 30-45%, when water content is less than 30-45%, carry out moisturizing of spraying water.
Described nutrient substance includes the KH of the carbamide of the composition of following percentage by weight: 5-10%, 1-5%2PO4, 1-5%
K2HPO4, the yeast extract of 0.5-1%, the trace element of 0.1-0.5%, remaining be water.
Described biosurfactant is rhamnolipid biological surface activator, main constituent in described biosurfactant
Concentration be 0.5-2%, remaining is water.
Described Pseudomonas aeruginosa uses aerobic liquid fermentation method, comprises the steps:
S1, will spread cultivation after seed liquor be forwarded in the fermentation tank culture medium after sterilizing according to the inoculum concentration that volume ratio is 3%-5%,
And regulate the pH value of fermentation medium to 6-7, keep temperature 30.5-31.5 DEG C, tank pressure 0.09-0.1MPa；
S2, being aerated, stir fermentation, wherein, ventilation is 30m3/ h, rotating speed is 120rpm；
S3, cultivation about 18h, function bacterium quantity >=10 of the microbial inoculum obtained after liquid fermentation8；
Wherein, wherein, the culture medium of liquid fermentation includes the composition of following percentage by weight: the peptone of 1%, the yeast of 0.5%
Cream, the water of the NaCl of 1% and 97.5%.
Described surfactant hydrocarbon degradation bacteria uses aerobic liquid fermentation method, comprises the steps:
S1, will spread cultivation after seed liquor be forwarded in the fermentation tank culture medium after sterilizing according to the inoculum concentration that volume ratio is 3%-5%,
And regulate the pH value of fermentation medium to 6-7, keep temperature 30.5-31.5 DEG C, tank pressure 0.09-0.1MPa；
S2, being aerated, stir fermentation, wherein, ventilation is 30m3/ h, rotating speed is 120rpm；
S3, cultivation 18h-24 h, function bacterium quantity >=10 of the microbial inoculum obtained after liquid fermentation8；
Wherein, the culture medium of liquid fermentation includes the composition of following percentage by weight: the peptone of 1%, the yeast extract of 0.5%, 1%
The water of NaCl and 97.5%.
Microorganism aerobic degradation process needs enough oxygen content, described oxygen by blower fan, gas distribution pipeline be passed through containing
The soil of mixed vaccine, meets microbial degradation process needs, and described gas distribution pipeline includes main line and bye-pass, main line and
Passage aperture on pipeline is 2-5mm, and the distance between adjacent vent holes is 4-6cm, and the ventilation of every cubic metre of soil is
3-5m3/h。
The present invention utilizes the method that biology in situ is repaired, and uses air injection, Pseudomonas aeruginosa and alkane degradation in soil
Bacterium, nutritional solution, the method for biosurfactant, utilize soil environment, microorganism using hydrocarbon contamination material as nutrient substance
Utilize, reach the purpose of biological restoration.The biological restoration time of the present invention regards hydrocarbon contamination degree, generally 2-4 individual month, compared with it
Its geobiont general about 1 year of time of process is compared, and action time is the shortest.
It addition, Pseudomonas aeruginosa and surfactant hydrocarbon degradation bacteria in the present invention are merits isolated and purified from the soil of oil pollution
Energy bacterium, both bacterium can utilize hydrocarbons, by adding biosurfactant, can accelerate the emulsifying of hydrocarbons pollutant
Effect, is preferably utilized by Pseudomonas aeruginosa, to improve speed and the degradation effect of degradation process, present invention process is simple,
Low cost, workload are little, and the product used by reparation is biological product, apply effective, environment will not be produced secondary pollution,
It is that a kind of gas station's contaminated soil that is suitable for administers effective biological reinforced restorative procedure, uses this method to carry out the soil of gas station
Biological restoration, general hydrocarbons pollutant content is less than 10%, and contaminant degradation rate can reach more than 98%.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Within god and principle, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.
Claims (10)
1. the biological reinforced restorative procedure of gas station's contaminated soil, it is characterised in that: comprise the steps:
S1, Pseudomonas aeruginosa and surfactant hydrocarbon degradation bacteria 1:1 by volume are configured to mixed vaccine；
S2, mixed vaccine is added contaminated soil, and mixed vaccine and contaminated soil are puddled uniformly, obtain the pollution containing mixed vaccine
Soil；
S3, the nutrient substance needed to contaminated soil addition mixed vaccine growth and breeding, and it is alive to add biological surface to contaminated soil
Property agent；And
S4, in contaminated soil, it is passed through oxygen.
The biological reinforced restorative procedure of gas station the most according to claim 1 contaminated soil, it is characterised in that: mix first
After closing bacterium addition soil and puddling uniformly with soil, nutrient substance and biosurfactant 1:1 by volume are entered to soil
Row uniformly sprays, and sprays once every 15 days later, and sprinkling ratio presses nutrient substance and biosurfactant volume ratio 1:1,
The addition of the mixed solution of described nutrient substance and biosurfactant is that every cubic metre of contaminated soil adds 5-10L.
The biological reinforced restorative procedure of gas station the most according to claim 1 contaminated soil, it is characterised in that: containing mixing
The water content control of the contaminated soil of bacterium, at 30-45%, when water content is less than 30-45%, carries out moisturizing of spraying water.
The biological reinforced restorative procedure of gas station the most according to claim 1 contaminated soil, it is characterised in that: in S1,
The cell concentration of described Pseudomonas aeruginosa and surfactant hydrocarbon degradation bacteria is all higher than 108Individual/g soil.
The biological reinforced restorative procedure of gas station the most according to claim 1 contaminated soil, it is characterised in that: in S2,
Described mixed vaccine addition is that every cubic metre of contaminated soil adds 10-20L.
The biological reinforced restorative procedure of gas station the most according to claim 1 contaminated soil, it is characterised in that: described Aerugo
Pseudomonas uses aerobic liquid fermentation method, comprises the steps:
S101, will spread cultivation after seed liquor be forwarded to the fermentation tank culture medium after sterilizing according to the inoculum concentration that volume ratio is 3%-5%
In, and regulate the pH value of fermentation medium to 6-7, keep temperature 30.5-31.5 DEG C, tank pressure 0.09-0.1MPa；
S102, being aerated, stir fermentation, wherein, ventilation is 30m3/ h, rotating speed is 120rpm；
S103, cultivation 18h-24h, function bacterium quantity >=10 of the microbial inoculum obtained after liquid fermentation8Individual；
Wherein, wherein, the culture medium of liquid fermentation includes the composition of following percentage by weight: the peptone of 1%, the yeast of 0.5%
Cream, the water of the NaCl of 1% and 97.5%.
The biological reinforced restorative procedure of gas station the most according to claim 1 contaminated soil, it is characterised in that: described hydrocarbon drops
Solve bacterium and use aerobic liquid fermentation method, comprise the steps:
S111, will spread cultivation after seed liquor be forwarded to the fermentation tank culture medium after sterilizing according to the inoculum concentration that volume ratio is 3%-5%
In, and regulate the pH value of fermentation medium to 6-7, keep temperature 30.5-31.5 DEG C, tank pressure 0.09-0.1MPa；
S112, being aerated, stir fermentation, wherein, ventilation is 30m3/ h, rotating speed is 120rpm；
S113, cultivation about 18h, function bacterium quantity >=10 of the microbial inoculum obtained after liquid fermentation8Individual；
Wherein, the culture medium of liquid fermentation includes the composition of following percentage by weight: the peptone of 1%, the yeast extract of 0.5%, 1%
The water of NaCl and 97.5%.
The biological reinforced restorative procedure of gas station the most according to claim 1 contaminated soil, it is characterised in that: described nutrition
Material includes the KH of the carbamide of the composition of following percentage by weight: 5-10%, 1-5%2PO4, the K of 1-5%2HPO4, the ferment of 0.5-1%
Female cream, the trace element of 0.1-0.5%, remaining be water.
The biological reinforced restorative procedure of gas station the most according to claim 1 contaminated soil, it is characterised in that: described biology
Surfactant is rhamnolipid biological surface activator, and in described biosurfactant, the concentration of main constituent is 0.5-2%,
Remaining is water.
The biological reinforced restorative procedure of gas station the most according to claim 1 contaminated soil, it is characterised in that: described oxygen
Gas is passed through the soil containing mixed vaccine by blower fan, gas distribution pipeline；Described gas distribution pipeline includes main line and bye-pass, main line and
Passage aperture on bye-pass is 2-5mm, and the distance between adjacent vent holes is 4-6cm, the ventilation of every cubic metre of soil
For 3-5m3/h。
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Cited By (2)
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| CN112980450A (en) * | 2021-02-05 | 2021-06-18 | 北京沃太斯环保科技发展有限公司 | Activator for in-situ remediation of deep polluted soil microorganisms and application thereof |
| CN113372170A (en) * | 2020-03-03 | 2021-09-10 | 和协环保科技股份有限公司 | Nutrient medium (carbon-based biological compound culture agent and carbon-based biological compound culture enhancer) for enhancing bioremediation of TPH (thermoplastic vulcanizate) polluted soil |
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