CN106053321A - Liquid flow system used for flow cytometry - Google Patents
Liquid flow system used for flow cytometry Download PDFInfo
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- CN106053321A CN106053321A CN201610655002.3A CN201610655002A CN106053321A CN 106053321 A CN106053321 A CN 106053321A CN 201610655002 A CN201610655002 A CN 201610655002A CN 106053321 A CN106053321 A CN 106053321A
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- 239000007788 liquid Substances 0.000 title claims abstract description 154
- 238000000684 flow cytometry Methods 0.000 title claims abstract description 26
- 239000002699 waste material Substances 0.000 claims abstract description 89
- 239000012530 fluid Substances 0.000 claims description 186
- 238000001514 detection method Methods 0.000 claims description 17
- 230000008676 import Effects 0.000 claims description 13
- 230000009977 dual effect Effects 0.000 claims description 9
- 230000037361 pathway Effects 0.000 claims description 9
- 230000005611 electricity Effects 0.000 claims description 2
- 239000003599 detergent Substances 0.000 description 5
- 238000004140 cleaning Methods 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 230000002572 peristaltic effect Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000019504 cigarettes Nutrition 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
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- Chemical & Material Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention provides a liquid flow system used for flow cytometry. The liquid flow system comprises a sheath liquid container, a waste liquid container, a vacuum pump, a flow chamber and a sample loading container, the gas discharge opening of the waste liquid container is connected with the vacuum pump through a gas circuit, the gas circuit is provided with an electric proportioning valve, the liquid inlet of the waste liquid container is connected with the waste liquid opening of the flow chamber through a waste liquid tube, the sample opening of the flow chamber is connected with the sample loading container, the sheath liquid inlet of the flow chamber is connected with the sheath liquid container through a sheath liquid tube, and the sheath liquid tube is provided with an electromagnetic pinch valve. The sheath liquid container and the sample container are not closed, and are convenient to install.
Description
Technical field
The invention belongs to biological detection equipment technical field, be specifically related to a kind of liquid fluid system for flow cytometry.
Background technology
In FCM analysis technology, stable sheath liquid stream, guarantee sample has stable gathering in flow chamber
Burnt effect.For avoid sheath fluid be contaminated, during engineering construction, have employing compressed air be power source, also have use peristaltic pump be
Power source.
Use malleation compressed gas to provide power for liquid, need to press, sheath fluid and sample therefore to sample simultaneously
The sealing installation requirement of container is high.The limitation using peristaltic pump is not only in that pump operationally can produce stream of pulses, causes gathering
Jiao is unstable, and employing flexible pipe not only can make to bear pressure and be restricted, and flexible pipe to be regularly replaced, its range of flow also compares
Narrow.
Summary of the invention
In order to solve above-mentioned technical problem, the invention provides a kind of liquid fluid system for flow cytometry.This liquid stream
System uses negative pressure air to be power, acts on waste fluid container with minipump, the sewer pipe of waste fluid container and flow chamber
Waste liquid outlet is connected, and makes sheath fluid and sample liquid are pulled out by flow chamber, forms liquid stream.
Technical scheme is as follows:
A kind of liquid fluid system for flow cytometry, including sheath liquid container, waste fluid container, vacuum pump, flow chamber and on
Sample container, the air vent of described waste fluid container connects described vacuum pump by gas circuit, described gas circuit is provided with dynamoelectric proportion valve,
The inlet of described waste fluid container is connected with the waste liquid port of described flow chamber by sewer pipe, and the specimen port of flow chamber connects described
Loading container, the sheath fluid import of flow chamber connects described sheath liquid container by sheath fluid pipe, described sheath fluid pipe is provided with electromagnetic clamp
Pipe valve.
Vacuum pump in system acts on waste fluid container, under the effect of negative pressure, makes the sample liquid in sheath fluid and loading container
It is pulled out by flow chamber, forms liquid stream；Regulated the size flow velocity with control waste liquid of negative pressure by dynamoelectric proportion valve simultaneously,
The sheath flow quantity of flow chamber it is adjusted into by electromagnetic clamp pipe valve.
Flow to also be sequentially provided with gas bubble detection and the first three-way magnetic valve along liquid on the sheath fluid pipe of the present invention, described the
The inlet of one three-way magnetic valve and the first liquid outlet connection sheath fluid pipe, the second liquid outlet of the first three-way magnetic valve connects described
Sewer pipe.
When sheath fluid pipe has bubble, gas bubble detection detecting signal, first three-way magnetic valve energising, the sheath fluid of bubbles by
I/O mouth flows to NC mouth, and the sheath fluid of bubbles is directly collected in waste fluid container, without being flowed in flow chamber.
Sheath liquid container of the present invention is additionally provided with the air inlet connecting air filter, it is to avoid impurity enters sheath fluid to be held
In device.
Preferably, the bottle cap of described sheath liquid container is provided with dual pathways snap joint, the difference being connected with sheath liquid container
It is atmospheric air filter and sheath fluid pipe, only in the situation using compressed air to substitute atmospheric pressure between snap joint and bottle cap,
Just require to seal.
It is provided with electromagnetic valve in gas circuit between air vent and the dynamoelectric proportion valve of waste fluid container of the present invention, described
Be additionally provided with the second three-way magnetic valve in gas circuit between dynamoelectric proportion valve and vacuum pump, and the second described three-way magnetic valve with
Dynamoelectric proportion valve forms closed-loop path.
During detergent line, load in loading container is washing liquid, and electromagnetic clamp pipe valve does not act on sheath fluid pipe so that it is standard-sized sheet,
Second three-way magnetic valve energising, the connection of NC mouth, negative pressure is not via the control of dynamoelectric proportion valve.After cleaning completes, make electromagnetism immediately
Valve is energized, and makes the negative pressure in waste fluid container unload, and the liquid in whole fluid path stops flowing, makes to be full of in liquid stream pipeline cleanout fluid
And sheath fluid.
Vacuum pump of the present invention is minipump.
Preferably, described minipump is the miniature brushless vacuum pump of resistance to 0-100 DEG C, it is possible to provide vacuum 65kPa, takes out
Beat dual-purpose, low interference, the long-life, carry overheated, the double protection of overload, the long-life, can work for continuous 24 hours, volume is little (about fragrant
Cigarette case size), weight only 330 grams.
Waste fluid container of the present invention is provided with dual pathways snap joint, be connected with waste fluid container be respectively sewer pipe and
From the negative-pressure gas of air pump, require between snap joint and bottle cap to seal.
Preferably, the first described three-way magnetic valve and the second three-way magnetic valve are diaphragm to dynamic formula rocking arm electromagnetism
Valve, valve body dead band is minimum, compact conformation, and fluid seals off with actuator, and gas-liquid is the most applicable.
Dynamoelectric proportion valve of the present invention is negative-pressure type proportioning valve, pressure limit 1 to 100kPa.Its input accesses
Carrying out the negative pressure of minipump, outfan accesses waste fluid container, controls to input to the vacuum of waste liquid bottle by voltage-regulation,
With coutroi velocity.
The beneficial effects of the present invention is:
1, system use vacuum pump act on waste fluid container, under the effect of negative pressure, in making sheath fluid and loading container
Sample liquid is pulled out by flow chamber, forms liquid stream；It is provided with dynamoelectric proportion valve to regulate the size of negative pressure, thus coutroi velocity；
Being provided with electromagnetic clamp pipe valve, pipe clip valve, for the cross-sectional area by changing sheath fluid pipe, is adjusted into the sheath liquid stream of flow chamber
Amount.Sheath liquid container and sample container are without airtight, easy for installation.
2, native system is provided with gas bubble detection and the first three-way magnetic valve on sheath fluid pipe, and gas bubble detection is used for detecting
Whether have bubble to flow through sheath fluid pipe, and provide corresponding signal to indicate the first three-way magnetic valve energising, the sheath fluid of bubbles is by I/O mouth
Flowing to NC mouth, the sheath fluid of bubbles is directly collected in waste fluid container, without being flowed in flow chamber, it is achieved going of bubble
Remove.
3, in native system also gas circuit between dynamoelectric proportion valve and vacuum pump, the second three-way magnetic valve, detergent line are set
Time, load in loading container is washing liquid, and electromagnetic clamp pipe valve does not act on sheath fluid pipe so that it is standard-sized sheet, and the second three-way magnetic valve leads to
Electricity, the connection of NC mouth, negative pressure is not via the control of dynamoelectric proportion valve.After cleaning completes, make the second three-way magnetic valve energising immediately, allow
I/O mouth is connected with NC mouth, makes the negative pressure in waste fluid container unload, and the liquid in whole fluid path stops flowing, makes in liquid stream pipeline
It is full of cleanout fluid and sheath fluid, it is achieved the cleaning of pipeline.
Accompanying drawing explanation
Fig. 1 is the structure chart of the liquid fluid system of embodiment 1 flow cytometer.
Fig. 2 is the structure chart of the liquid fluid system of embodiment 2-10 flow cytometer.
Figure is labeled as: 101, waste fluid container, 102, sheath liquid container, 201, dual pathways snap joint, 301, electromagnetic valve,
302, the second three-way magnetic valve, the 303, first three-way magnetic valve, 4, dynamoelectric proportion valve, 5, vacuum pump, 6, flow chamber, 601, sample
Mouthful, 602, sheath fluid import, 603, waste liquid port, 7, loading container, 8, electromagnetic clamp pipe valve, 9, gas bubble detection, 10, air filtration
Device.
Detailed description of the invention
All features disclosed in this specification, or disclosed all methods or during step, except mutually exclusive
Feature and/or step beyond, all can combine by any way.
Any feature disclosed in this specification (including any accessory claim, summary and accompanying drawing), unless chatted especially
State, all can be by other equivalences or there is the alternative features of similar purpose replaced.I.e., unless specifically stated otherwise, each feature is only
It it is an example in a series of equivalence or similar characteristics.
Embodiment 1
As it is shown in figure 1, a kind of liquid fluid system for flow cytometry, including sheath liquid container 102, waste fluid container 101, true
Empty pump 5, flow chamber 6 and loading container 7, the air vent of described waste fluid container 101 connects described vacuum pump 5, institute by gas circuit
Stating and be provided with dynamoelectric proportion valve 4 in gas circuit, the inlet of described waste fluid container 101 is by the waste liquid of sewer pipe with described flow chamber
Mouth 603 connects, and the specimen port 601 of flow chamber connects described loading container 7, and the sheath fluid import 602 of flow chamber is by sheath fluid Guan Lian
Connect described sheath liquid container 102, described sheath fluid pipe is provided with electromagnetic clamp pipe valve 8.
Embodiment 2
As in figure 2 it is shown, a kind of liquid fluid system for flow cytometry, including sheath liquid container 102, waste fluid container 101, true
Empty pump 5, flow chamber 6 and loading container 7, the air vent of described waste fluid container 101 connects described vacuum pump 5, institute by gas circuit
Stating and be provided with dynamoelectric proportion valve 4 in gas circuit, the inlet of described waste fluid container 101 is by the waste liquid of sewer pipe with described flow chamber
Mouth 603 connects, and the specimen port 601 of flow chamber connects described loading container 7, and the sheath fluid import 602 of flow chamber is by sheath fluid Guan Lian
Connect described sheath liquid container 102, described sheath fluid pipe is provided with electromagnetic clamp pipe valve 8.
Flow to also be sequentially provided with gas bubble detection 9 and the first three-way magnetic valve 303 along liquid on described sheath fluid pipe, described
The inlet of the first three-way magnetic valve 303 and the first liquid outlet connection sheath fluid pipe, the second liquid outlet of the first three-way magnetic valve 303
Connect described sewer pipe.
Embodiment 3
As in figure 2 it is shown, a kind of liquid fluid system for flow cytometry, including sheath liquid container 102, waste fluid container 101, true
Empty pump 5, flow chamber 6 and loading container 7, the air vent of described waste fluid container 101 connects described vacuum pump 5, institute by gas circuit
Stating and be provided with dynamoelectric proportion valve 4 in gas circuit, the inlet of described waste fluid container 101 is by the waste liquid of sewer pipe with described flow chamber
Mouth 603 connects, and the specimen port 601 of flow chamber connects described loading container 7, and the sheath fluid import 602 of flow chamber is by sheath fluid Guan Lian
Connect described sheath liquid container 102, described sheath fluid pipe is provided with electromagnetic clamp pipe valve 8.
Flow to also be sequentially provided with gas bubble detection 9 and the first three-way magnetic valve 303 along liquid on described sheath fluid pipe, described
The inlet of the first three-way magnetic valve 303 and the first liquid outlet connection sheath fluid pipe, the second liquid outlet of the first three-way magnetic valve 303
Connect described sewer pipe.
Described sheath liquid container 102 is additionally provided with the air inlet connecting air filter 10.
Embodiment 4
As in figure 2 it is shown, a kind of liquid fluid system for flow cytometry, including sheath liquid container 102, waste fluid container 101, true
Empty pump 5, flow chamber 6 and loading container 7, the air vent of described waste fluid container 101 connects described vacuum pump 5, institute by gas circuit
Stating and be provided with dynamoelectric proportion valve 4 in gas circuit, the inlet of described waste fluid container 101 is by the waste liquid of sewer pipe with described flow chamber
Mouth 603 connects, and the specimen port 601 of flow chamber connects described loading container 7, and the sheath fluid import 602 of flow chamber is by sheath fluid Guan Lian
Connect described sheath liquid container 102, described sheath fluid pipe is provided with electromagnetic clamp pipe valve 8.
Flow to also be sequentially provided with gas bubble detection 9 and the first three-way magnetic valve 303 along liquid on described sheath fluid pipe, described
The inlet of the first three-way magnetic valve 303 and the first liquid outlet connection sheath fluid pipe, the second liquid outlet of the first three-way magnetic valve 303
Connect described sewer pipe.
Described sheath liquid container 102 is additionally provided with the air inlet connecting air filter 10.
It is provided with dual pathways snap joint 201 on the bottle cap of described sheath liquid container 102.
Embodiment 5
As in figure 2 it is shown, a kind of liquid fluid system for flow cytometry, including sheath liquid container 102, waste fluid container 101, true
Empty pump 5, flow chamber 6 and loading container 7, the air vent of described waste fluid container 101 connects described vacuum pump 5, institute by gas circuit
Stating and be provided with dynamoelectric proportion valve 4 in gas circuit, the inlet of described waste fluid container 101 is by the waste liquid of sewer pipe with described flow chamber
Mouth 603 connects, and the specimen port 601 of flow chamber connects described loading container 7, and the sheath fluid import 602 of flow chamber is by sheath fluid Guan Lian
Connect described sheath liquid container 102, described sheath fluid pipe is provided with electromagnetic clamp pipe valve 8.
Flow to also be sequentially provided with gas bubble detection 9 and the first three-way magnetic valve 303 along liquid on described sheath fluid pipe, described
The inlet of the first three-way magnetic valve 303 and the first liquid outlet connection sheath fluid pipe, the second liquid outlet of the first three-way magnetic valve 303
Connect described sewer pipe.
It is provided with electromagnetic valve 301, electronic ratio in gas circuit between air vent and the dynamoelectric proportion valve 4 of described waste fluid container
Be additionally provided with the second three-way magnetic valve 302 in gas circuit between valve 4 and vacuum pump 5, and the second described three-way magnetic valve 302 with
Dynamoelectric proportion valve 4 forms closed-loop path.
During normal work, gas flow is: air → sheath liquid container 102；
NO mouth → the two or three of waste fluid container 101 → electromagnetic valve, 301 → dynamoelectric proportion valve 4 → the second three-way magnetic valve 302
The I/O mouth of three-way electromagnetic valve → vacuum pump 5 air inlet.
During normal work, liquid flows to: sample liquid is through the specimen port 601 of loading container 7 → flow chamber 6；
The NO mouth of I/O mouth → the first three-way magnetic valve of sheath fluid → the first three-way magnetic valve 303 in sheath liquid container 102 →
Waste liquid port 603 → the waste fluid container 101 of the sheath fluid mouth 602 → flow chamber 6 of flow chamber 6.
When sheath fluid pipe has bubble, gas bubble detection 9 detecting signal, the first three-way magnetic valve 303 is energized, the sheath of bubbles
Liquid is flowed to NC mouth by I/O mouth, may not flow in flow chamber 6, and the sheath fluid of last bubbles is directly collected into waste fluid container 101
In.During bubble removing, stream condition is consistent with during normal work.
During detergent line, what loading container 7 loaded is washing liquid, and electromagnetic clamp pipe valve 9 does not act on sheath fluid pipe so that it is standard-sized sheet.
Second three-way magnetic valve 302 is energized, and NC mouth is connected, and negative pressure is not via the control of dynamoelectric proportion valve 4.After cleaning completes, close immediately
Closing electromagnetic valve 301, make the negative pressure in waste fluid container 201 unload, the liquid in whole fluid path stops flowing, makes to fill in liquid stream pipeline
Qing Dynasty's washing liquid and sheath fluid.
Gas flow during detergent line: air → sheath liquid container 102；
The I/O mouth of the NC mouth → three-way magnetic valve 302 of waste fluid container 101 → electromagnetic valve 301 → the second three-way magnetic valve 302
→ minipump 5 air inlet.
During detergent line, liquid flows to: cleanout fluid is through the specimen port 601 of loading container 7 → flow chamber 6；Sheath liquid container
The sheath fluid mouth of the NO mouth → flow chamber 6 of I/O mouth → the first three-way magnetic valve of sheath fluid → the first three-way magnetic valve 303 in 102
Waste liquid port 603 → the waste fluid container 101 of 602 → flow chamber 6.
Embodiment 6
As in figure 2 it is shown, a kind of liquid fluid system for flow cytometry, including sheath liquid container 102, waste fluid container 101, true
Empty pump 5, flow chamber 6 and loading container 7, the air vent of described waste fluid container 101 connects described vacuum pump 5, institute by gas circuit
Stating and be provided with dynamoelectric proportion valve 4 in gas circuit, the inlet of described waste fluid container 101 is by the waste liquid of sewer pipe with described flow chamber
Mouth 603 connects, and the specimen port 601 of flow chamber connects described loading container 7, and the sheath fluid import 602 of flow chamber is by sheath fluid Guan Lian
Connect described sheath liquid container 102, described sheath fluid pipe is provided with electromagnetic clamp pipe valve 8.
Flow to also be sequentially provided with gas bubble detection 9 and the first three-way magnetic valve 303 along liquid on described sheath fluid pipe, described
The inlet of the first three-way magnetic valve 303 and the first liquid outlet connection sheath fluid pipe, the second liquid outlet of the first three-way magnetic valve 303
Connect described sewer pipe.
It is provided with electromagnetic valve 301, electronic ratio in gas circuit between air vent and the dynamoelectric proportion valve 4 of described waste fluid container
Be additionally provided with the second three-way magnetic valve 302 in gas circuit between valve 4 and vacuum pump 5, and the second described three-way magnetic valve 302 with
Dynamoelectric proportion valve 4 forms closed-loop path.
Described vacuum pump 5 is minipump.
Embodiment 7
As in figure 2 it is shown, a kind of liquid fluid system for flow cytometry, including sheath liquid container 102, waste fluid container 101, true
Empty pump 5, flow chamber 6 and loading container 7, the air vent of described waste fluid container 101 connects described vacuum pump 5, institute by gas circuit
Stating and be provided with dynamoelectric proportion valve 4 in gas circuit, the inlet of described waste fluid container 101 is by the waste liquid of sewer pipe with described flow chamber
Mouth 603 connects, and the specimen port 601 of flow chamber connects described loading container 7, and the sheath fluid import 602 of flow chamber is by sheath fluid Guan Lian
Connect described sheath liquid container 102, described sheath fluid pipe is provided with electromagnetic clamp pipe valve 8.
Flow to also be sequentially provided with gas bubble detection 9 and the first three-way magnetic valve 303 along liquid on described sheath fluid pipe, described
The inlet of the first three-way magnetic valve 303 and the first liquid outlet connection sheath fluid pipe, the second liquid outlet of the first three-way magnetic valve 303
Connect described sewer pipe.
It is provided with electromagnetic valve 301, electronic ratio in gas circuit between air vent and the dynamoelectric proportion valve 4 of described waste fluid container
Be additionally provided with the second three-way magnetic valve 302 in gas circuit between valve 4 and vacuum pump 5, and the second described three-way magnetic valve 302 with
Dynamoelectric proportion valve 4 forms closed-loop path.
The described miniature brushless vacuum pump that vacuum pump 5 is resistance to 0-100 DEG C.
Described dynamoelectric proportion valve 4 is negative-pressure type proportioning valve, pressure limit 20kPa.
Embodiment 8
As in figure 2 it is shown, a kind of liquid fluid system for flow cytometry, including sheath liquid container 102, waste fluid container 101, true
Empty pump 5, flow chamber 6 and loading container 7, the air vent of described waste fluid container 101 connects described vacuum pump 5, institute by gas circuit
Stating and be provided with dynamoelectric proportion valve 4 in gas circuit, the inlet of described waste fluid container 101 is by the waste liquid of sewer pipe with described flow chamber
Mouth 603 connects, and the specimen port 601 of flow chamber connects described loading container 7, and the sheath fluid import 602 of flow chamber is by sheath fluid Guan Lian
Connect described sheath liquid container 102, described sheath fluid pipe is provided with electromagnetic clamp pipe valve 8.
Flow to also be sequentially provided with gas bubble detection 9 and the first three-way magnetic valve 303 along liquid on described sheath fluid pipe, described
The inlet of the first three-way magnetic valve 303 and the first liquid outlet connection sheath fluid pipe, the second liquid outlet of the first three-way magnetic valve 303
Connect described sewer pipe.
It is provided with electromagnetic valve 301, electronic ratio in gas circuit between air vent and the dynamoelectric proportion valve 4 of described waste fluid container
Be additionally provided with the second three-way magnetic valve 302 in gas circuit between valve 4 and vacuum pump 5, and the second described three-way magnetic valve 302 with
Dynamoelectric proportion valve 4 forms closed-loop path.
The described miniature brushless vacuum pump that vacuum pump 5 is resistance to 0-100 DEG C.
Described waste fluid container 101 is provided with dual pathways snap joint.
Described dynamoelectric proportion valve 4 is negative-pressure type proportioning valve, pressure limit-50kPa.
Embodiment 9
As in figure 2 it is shown, a kind of liquid fluid system for flow cytometry, including sheath liquid container 102, waste fluid container 101, true
Empty pump 5, flow chamber 6 and loading container 7, the air vent of described waste fluid container 101 connects described vacuum pump 5, institute by gas circuit
Stating and be provided with dynamoelectric proportion valve 4 in gas circuit, the inlet of described waste fluid container 101 is by the waste liquid of sewer pipe with described flow chamber
Mouth 603 connects, and the specimen port 601 of flow chamber connects described loading container 7, and the sheath fluid import 602 of flow chamber is by sheath fluid Guan Lian
Connect described sheath liquid container 102, described sheath fluid pipe is provided with electromagnetic clamp pipe valve 8.
Flow to also be sequentially provided with gas bubble detection 9 and the first three-way magnetic valve 303 along liquid on described sheath fluid pipe, described
The inlet of the first three-way magnetic valve 303 and the first liquid outlet connection sheath fluid pipe, the second liquid outlet of the first three-way magnetic valve 303
Connect described sewer pipe.
It is provided with electromagnetic valve 301, electronic ratio in gas circuit between air vent and the dynamoelectric proportion valve 4 of described waste fluid container
Be additionally provided with the second three-way magnetic valve 302 in gas circuit between valve 4 and vacuum pump 5, and the second described three-way magnetic valve 302 with
Dynamoelectric proportion valve 4 forms closed-loop path.
The described miniature brushless vacuum pump that vacuum pump 5 is resistance to 0-100 DEG C.
Described waste fluid container 101 is provided with dual pathways snap joint.
The first described three-way magnetic valve 303 and the second three-way magnetic valve 302 are diaphragm to dynamic formula rocking arm electromagnetic valve.
Described dynamoelectric proportion valve 4 is negative-pressure type proportioning valve, pressure limit 1kPa.
Embodiment 10
As in figure 2 it is shown, a kind of liquid fluid system for flow cytometry, including sheath liquid container 102, waste fluid container 101, true
Empty pump 5, flow chamber 6 and loading container 7, the air vent of described waste fluid container 101 connects described vacuum pump 5, institute by gas circuit
Stating and be provided with dynamoelectric proportion valve 4 in gas circuit, the inlet of described waste fluid container 101 is by the waste liquid of sewer pipe with described flow chamber
Mouth 603 connects, and the specimen port 601 of flow chamber connects described loading container 7, and the sheath fluid import 602 of flow chamber is by sheath fluid Guan Lian
Connect described sheath liquid container 102, described sheath fluid pipe is provided with electromagnetic clamp pipe valve 8.
Flow to also be sequentially provided with gas bubble detection 9 and the first three-way magnetic valve 303 along liquid on described sheath fluid pipe, described
The inlet of the first three-way magnetic valve 303 and the first liquid outlet connection sheath fluid pipe, the second liquid outlet of the first three-way magnetic valve 303
Connect described sewer pipe.
It is provided with electromagnetic valve 301 in gas circuit between air vent and the dynamoelectric proportion valve 4 of described waste fluid container 101, electronic
The second three-way magnetic valve 302, and the second described three-way magnetic valve it is additionally provided with in gas circuit between proportioning valve 4 and vacuum pump 5
302 form closed-loop path with dynamoelectric proportion valve 4.
The described miniature brushless vacuum pump that vacuum pump 5 is resistance to 0-100 DEG C.
Described waste fluid container 101 is provided with dual pathways snap joint.
The first described three-way magnetic valve 303 and the second three-way magnetic valve 302 are diaphragm to dynamic formula rocking arm electromagnetic valve.
Described dynamoelectric proportion valve 4 is negative-pressure type proportioning valve, pressure limit 100kPa.
Claims (10)
1. the liquid fluid system for flow cytometry, it is characterised in that include sheath liquid container, waste fluid container, vacuum pump, stream
Dynamic room and loading container, the air vent of described waste fluid container connects described vacuum pump by gas circuit, described gas circuit is provided with electricity
Dynamic proportioning valve, the inlet of described waste fluid container is connected with the waste liquid port of described flow chamber by sewer pipe, the sample of flow chamber
Mouth connects described loading container, and the sheath fluid import of flow chamber connects described sheath liquid container, described sheath fluid Guan Shangshe by sheath fluid pipe
It is equipped with electromagnetic clamp pipe valve.
A kind of liquid fluid system for flow cytometry the most according to claim 1, it is characterised in that described sheath fluid pipe
On flow to also be sequentially provided with gas bubble detection and the first three-way magnetic valve along liquid, the inlet of described first three-way magnetic valve and
First liquid outlet connection sheath fluid pipe, the second liquid outlet of the first three-way magnetic valve connects described sewer pipe.
A kind of liquid fluid system for flow cytometry the most according to claim 1, it is characterised in that described sheath fluid holds
Device is additionally provided with the air inlet connecting air filter.
A kind of liquid fluid system for flow cytometry the most according to claim 3, it is characterised in that described sheath liquid container
Bottle cap on be provided with dual pathways snap joint.
A kind of liquid fluid system for flow cytometry the most according to claim 1, it is characterised in that described waste fluid container
Air vent and dynamoelectric proportion valve between gas circuit on be provided with electromagnetic valve, the gas between described dynamoelectric proportion valve and vacuum pump
It is additionally provided with the second three-way magnetic valve on road, and the second described three-way magnetic valve forms closed-loop path with dynamoelectric proportion valve.
A kind of liquid fluid system for flow cytometry the most according to claim 1, it is characterised in that described vacuum pump
For minipump.
A kind of liquid fluid system for flow cytometry the most according to claim 6, it is characterised in that described miniature very
Empty pump is the miniature brushless vacuum pump of resistance to 0-100 DEG C.
A kind of liquid fluid system for flow cytometry the most according to claim 1, it is characterised in that described waste fluid container
It is provided with dual pathways snap joint.
9. according to a kind of liquid fluid system for flow cytometry described in any one in claim 2-8, it is characterised in that
The first described three-way magnetic valve and the second three-way magnetic valve are diaphragm to dynamic formula rocking arm electromagnetic valve.
A kind of liquid fluid system for flow cytometry the most according to claim 1, it is characterised in that described is electronic
Proportioning valve is negative-pressure type proportioning valve, pressure limit 1 to 100kPa.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610655002.3A CN106053321A (en) | 2016-08-10 | 2016-08-10 | Liquid flow system used for flow cytometry |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610655002.3A CN106053321A (en) | 2016-08-10 | 2016-08-10 | Liquid flow system used for flow cytometry |
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| CN106053321A true CN106053321A (en) | 2016-10-26 |
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| CN201610655002.3A Pending CN106053321A (en) | 2016-08-10 | 2016-08-10 | Liquid flow system used for flow cytometry |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106872340A (en) * | 2017-02-27 | 2017-06-20 | 赛雷纳（中国）医疗科技有限公司 | A kind of liquid fluid system for FCM analysis |
| CN107782657A (en) * | 2017-12-08 | 2018-03-09 | 成都索尔恩科技有限公司 | A kind of flow cytometer liquid fluid system and its control method |
| CN108627449A (en) * | 2017-03-23 | 2018-10-09 | 深圳市帝迈生物技术有限公司 | A kind of flow cytometer fluid system and measurement method |
| US10578220B2 (en) | 2017-02-27 | 2020-03-03 | Bimba Manufacturing Company | Proportionally controlled pinch valves, systems and methods |
| CN112858661A (en) * | 2019-11-28 | 2021-05-28 | 深圳市帝迈生物技术有限公司 | Sample detection system and sample analyzer |
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| US10578220B2 (en) | 2017-02-27 | 2020-03-03 | Bimba Manufacturing Company | Proportionally controlled pinch valves, systems and methods |
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| CN107782657B (en) * | 2017-12-08 | 2023-09-26 | 成都索尔恩科技有限公司 | Flow cytometer liquid flow system and control method thereof |
| CN112858661A (en) * | 2019-11-28 | 2021-05-28 | 深圳市帝迈生物技术有限公司 | Sample detection system and sample analyzer |
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