CN105817275A - Centrifugal runner device - Google Patents

Abstract

The invention discloses a centrifugal flow passage device. The centrifugal flow channel device comprises a flow channel body, a collecting unit and a waste liquid tank. The runner body is provided with a first surface and a second surface which are oppositely arranged. The flow channel body comprises a sample injection port, a sample flow channel, a separation groove, a reagent injection port, a reagent flow channel and a mixing flow channel. The sample injection port is configured on the first surface. The sample runner is connected with the sample injection port. The separation groove is adjacently arranged in the sample flow passage and is communicated with the sample flow passage. The reagent injection port is disposed on the first surface. The reagent flow channel is connected with the reagent injection port. One end of the mixing flow passage is connected with the sample flow passage and the reagent flow passage. The collecting unit is provided with an opening and at least one overflow hole, and the opening is communicated with the other end of the mixing flow channel. The waste liquid groove is communicated with the overflow hole of the collecting unit. When the sample to be detected flows through the junction of the sample flow channel and the separation groove, substances in the sample to be detected are separated under the action of centrifugal force and the screening of the density gradient solution.

Description

Centrifugal flow channel device

technical field

The invention relates to a flow channel device, in particular to a centrifugal flow channel device.

Background technique

No matter in clinical medicine or food inspection, there is a need to detect specific biomolecules. Specifically, in clinical medicine, by detecting the content of various biomolecules in the human body, such as free cells in body fluids such as blood and urine, or the content of various types of proteins, it is possible to initially evaluate whether the various organs of the human body are functioning normally. In terms of food inspection, it is even possible to preliminarily assess whether the food contains excessive carcinogens, pesticide residues, or identify genetically modified foods by testing the substances in food raw materials or products. However, before testing, it is necessary to separate or purify specific biomolecules or substances in the specimen samples (such as the aforementioned blood, urine, and food raw materials or products, etc.) to increase the concentration of the specific target substance. It is beneficial for the subsequent detection experiments. Therefore, the processing and separation of specimen samples is the primary goal of improving biomedical testing technology.

At present, microfluid technology has been applied to substances in the separation or purification of samples, such as centrifugal-based microfluidic devices. Centrifugal microfluidic devices separate or purify substances in a sample by centrifugation generated by rotation. However, in operation, the centrifugal microfluidic device can only inject a small amount of sample each time, so it is difficult to apply to process a large number of sample samples, and it is also unable to detect substances with low content in the sample samples. As a result, centrifugal microfluidic devices have had rather limited commercial success and generally only serve as research tools.

In addition, although there is currently an improved centrifugal microfluidic device that can process a large number of specimen samples through continuous injection, it only has a discharge channel for a large number of separated specimen samples, so that the separated The specimen samples are sprayed to the periphery due to centrifugation, which is prone to contamination.

Contents of the invention

In view of the above problems, the object of the present invention is to provide a centrifugal flow channel device, which can separate substances with different weights or sizes through centrifugal action and density gradient, and through the design of the collection unit and the waste liquid tank, it can be easily A large amount of specimen samples can be processed efficiently, and at the same time, the overall cleanliness of the centrifugal flow channel device can be maintained, and contamination can be avoided.

To achieve the above purpose, a centrifugal flow channel device according to the present invention includes a flow channel body, at least one collection unit and a waste liquid tank. The flow channel body has a first surface and a second surface oppositely arranged, and the flow channel body includes a sample injection port, at least one sample flow channel, a separation tank, a reagent injection port, at least one reagent flow channel and at least one mixed flow road. The sample injection port is arranged on the first surface. The sample flow channel is connected to the sample injection port. The separation groove is adjacent to the sample flow channel and communicated with the sample flow channel. The reagent injection port is configured on the first surface. The reagent flow channel is connected to the reagent injection port. One end of the mixing channel is connected to the sample channel and the reagent channel. The collecting unit has an opening and at least one overflow hole, and the opening communicates with the other end of the mixing channel. The waste liquid tank communicates with the overflow hole of the collection unit.

In one embodiment, the overflow hole of the collection unit is located on the second surface, and the waste liquid tank is connected to the second surface of the flow channel body.

In one embodiment, the overflow hole of the collection unit is located outside the flow channel body, and the waste liquid tank is connected to the outside of the flow channel body.

In one embodiment, the waste liquid tank is detachably arranged on the flow channel body.

In one embodiment, the waste liquid tank has an inner side wall and an outer side wall, the second surface of the flow channel body has at least two hooks, and the top edge of the inner side wall and the top edge of the outer side wall each have at least one hook. The two hooks are engaged with the two card slots.

In one embodiment, the second surface of the flow channel body has at least two protrusions extending from the hook to two sides to surround the outer periphery of the flow channel body. The two hooks and the two protrusions are jointly arranged on the The two card slots of the waste liquid tank.

In one embodiment, the flow channel body further has two elastic pieces disposed in the two engaging grooves.

In one embodiment, the collection unit is detachably disposed on the channel body.

In one embodiment, the flow channel body has a cavity or an opening, and the collecting unit is disposed in the cavity or the opening.

In one embodiment, the flow channel body has two cylinders, which are respectively arranged on the periphery of the sample injection port and the reagent injection port.

In one embodiment, the flow channel body is in the shape of a circular disk, and the waste liquid tank is an annular groove, which is arranged on the second surface of the flow channel body, or is arranged around the outside of the flow channel body.

In one embodiment, the sample channel partially extends outward to form a separation groove.

In one embodiment, the sample injection port is disposed at the geometric center of the flow channel body, and the sample flow channel extends outward in a helical shape.

To achieve the above purpose, a centrifugal flow channel device according to the present invention includes a flow channel body, at least one collection unit and a waste liquid tank. The flow channel body has a first surface and a second surface oppositely arranged, and the flow channel body includes at least one sample flow channel, at least one reagent flow channel and at least one mixing flow channel. The sample injection port is arranged inside the flow channel body, the sample flow channel forms a sample injection port on the first surface, and part of the sample flow channel extends outward to form a separation groove. The reagent flow channel is arranged inside the flow channel body, and the reagent flow channel forms a reagent injection port on the first surface. One end of the mixing channel is connected to the sample channel and the reagent channel. The collecting unit has an opening and at least one overflow hole, and the opening communicates with the other end of the mixing channel. The waste liquid tank communicates with the overflow hole of the collecting unit.

To achieve the above purpose, a centrifugal flow channel device according to the present invention includes a flow channel body, a waste liquid tank and at least one collection unit. The flow channel body has a first surface and a second surface oppositely arranged, and the flow channel body includes a sample injection port, at least one sample flow channel, a separation tank, a reagent injection port, at least one reagent flow channel, and at least one mixed flow Road and at least one first connecting part. The sample injection port is arranged on the first surface. The sample flow channel is connected to the sample injection port. The separation groove is adjacent to the sample flow channel and communicated with the sample flow channel. The reagent injection port is configured on the first surface. The reagent flow channel is connected to the reagent injection port. One end of the mixing channel is connected to the sample channel and the reagent channel. The first connecting portion is disposed on the second surface. The waste liquid tank is detachably arranged on the flow channel body, and the waste liquid tank has at least one second connecting part, and the first connecting part and the second connecting part cooperate with each other. The collecting unit has an opening and at least one overflow hole, the opening communicates with the other end of the mixing channel, and the overflow communicates with the waste liquid tank.

In one embodiment, the first connecting portion is a hook, and the second connecting portion is a slot.

In one embodiment, the waste liquid tank has an inner wall and an outer wall, the second surface of the flow channel body has at least two hooks, and the top edge of the inner wall and the top edge of the outer wall each have at least one hook. , the two hooks are engaged with the two slots.

In one embodiment, the second surface of the flow channel body has at least two protrusions extending from the hook to two sides to surround the outer periphery of the flow channel body. The two hooks and the two protrusions are jointly arranged on the The two card slots of the waste liquid tank.

In one embodiment, the flow channel body further has two elastic pieces disposed in the two engaging grooves.

In one embodiment, the flow channel body is in the shape of a circular disk, and the waste liquid tank is an annular groove, which is arranged on the second surface of the flow channel body, or is arranged around the outside of the flow channel body.

Based on the above, according to the centrifugal flow channel device of the present invention, through the design of the flow channel, especially the configuration relationship between the sample flow channel and the separation tank, when the specimen sample flows through the junction of the sample flow channel and the separation tank , through the action of centrifugal force and the screening of the density gradient solution, the lighter cells or molecules are driven by centrifugal force to flow to the mixing channel, while the heavier cells or molecules are washed and settled in the separation tank, and then Achieve the effect of separating substances in the specimen sample.

In addition, the waste liquid tank is designed to communicate with the overflow hole of the collection unit, so that the waste liquid tank can accept too many specimen samples, and then can easily process a large number of specimen samples, and at the same time maintain the centrifugal flow channel device as a whole cleaning to avoid contamination.

Description of drawings

Fig. 1 is a schematic diagram of a centrifugal channel device according to the first embodiment of the present invention.

FIG. 2 is a three-dimensional cross-sectional view of the centrifugal channel device shown in FIG. 1 .

FIG. 3 is a schematic view of the flow channel of the centrifugal flow channel device shown in FIG. 1 .

FIG. 4 is an enlarged schematic view of the collection unit and part of the mixing channel shown in FIG. 2 .

Fig. 5 is a schematic diagram of a centrifugal channel device according to the second embodiment of the present invention.

FIG. 6 is a schematic diagram of a centrifugal channel device according to a third embodiment of the present invention.

Fig. 7 is a schematic diagram of a centrifugal channel device according to the fourth embodiment of the present invention

FIG. 8A is a schematic diagram of a centrifugal channel device according to the fifth embodiment of the present invention.

FIG. 8B is an enlarged schematic view of the collection unit and part of the waste liquid tank shown in FIG. 8A .

FIG. 8C is an exploded schematic view of the collection unit and the waste liquid tank shown in FIG. 8B .

FIG. 9A is a schematic diagram of a centrifugal channel device according to the sixth embodiment of the present invention.

Fig. 9B is a side view of the centrifugal channel device shown in Fig. 9A.

FIG. 10A is a schematic diagram of a centrifugal channel device according to the seventh embodiment of the present invention.

FIG. 10B is a top view of the centrifugal channel device shown in FIG. 10A .

FIG. 11A is a cross-sectional view of the centrifugal flow channel device shown in FIG. 10A along line A-A.

FIG. 11B is an exploded schematic view of the centrifugal channel device shown in FIG. 11A .

FIG. 12A is a cross-sectional view of the centrifugal channel device shown in FIG. 10A along the section line B-B.

FIG. 12B is an exploded schematic view of the centrifugal channel device shown in FIG. 12A .

Each reference numeral in the figure is explained as follows.

1, 1a, 1b, 1c, 1d, 1e, 1f: runner body

10a, 10b, 10c: Opening

11: First Surface

12, 12f: second surface

13, 13a, 13b, 13c, 13d, 13e, 13f: sample injection ports

14, 14a, 14b, 14c, 14d, 14e, 14f: sample flow path

15, 15a, 15b, 15c, 15d, 15e, 15f: separation tank

16, 16a, 16b, 16c, 16d, 16e, 16f: Reagent injection port

17, 17a, 17b, 17c, 17d, 17e, 17f: Reagent channel

18, 18a, 18b, 18c, 18d, 18e, 18f: mixed flow channel

181: Confluence Point

182: Microstructure

19e: column

2, 2a, 2b, 2c, 2d, 2e, 2f: collection unit

21, 21c, 21d: opening

22, 22c, 22d: overflow hole

23, 23c, 23d: accommodation space

3, 3a, 3b, 3c, 3d, 3e, 3f: waste liquid tank

31: top surface

32: convex part

33d, 33f: inner wall

331f, 341f: card slot

34f: Outer wall

A: low density area

B: high density area

C1, C2, C3, C4, C5, C6, C7: centrifugal flow channel device

H: open hole

HC: large cell

LC: small cell

M1: The first runner body

M11: groove

M11, M21: Hook

M12, M22: convex part

M2: Second runner body

O: positioning hole

detailed description

A centrifugal channel device according to a preferred embodiment of the present invention will be described below with reference to related drawings, wherein the same components will be described with the same reference symbols.

FIG. 1 is a schematic diagram of a centrifugal channel device according to the first embodiment of the present invention, please refer to FIG. 1 . The centrifugal flow channel device C1 of this embodiment can be applied to process different types of specimen samples, and the specimen samples are fluids, such as but not limited to blood samples, plasma fluid, urine or other body fluids and other fluid biological Specimen samples. In addition, the centrifugal flow channel device C1 of this embodiment can be applied to the specimen sample with a lower concentration of the detection target (specific biomolecule or substance), that is, the content of the detection target present in the specimen sample is less, so It is necessary to obtain a sufficient amount of detection targets by inputting a large number of specimen samples, and the centrifugal flow channel device C1 of this embodiment can be applied to process a large number of specimen samples.

For example, in the process of tumor metastasis (Tumor Metastasis), which refers to the process of tumor cells transferring from the original site to other parts of the body far away from the original site (such as other tissues or organs) to continue to grow, the tumor cells must first Overcome or bypass adjacent cells, enter the circulatory system, such as blood vessels, and transfer to other tissues or organs through the blood circulatory system, and the tumor cells that are free in the blood are called "circulating tumor cells (CTC) ". Low content of circulating tumor cells in the blood, that is, in the blood sample, compared with other blood cells, the proportion of circulating tumor cells in the overall composition of the blood sample is very low, even in patients with metastatic tumors In the blood, there is usually only one circulating tumor cell in every 106-109 monocytes. Therefore, it is necessary to process a large number of blood samples (specimen samples) so as to obtain a sufficient amount of circulating tumor cells (detection targets), and the centrifugal flow channel device 1 of this embodiment is based on the separation of circulating tumor cells in blood samples. Example.

FIG. 2 is a three-dimensional cross-sectional view of the centrifugal flow channel device shown in FIG. 1 , please refer to FIG. 1 and FIG. 2 at the same time. The centrifugal channel device C1 includes a channel body 1 , at least one collection unit 2 and a waste liquid tank 3 . The runner body 1 of this embodiment can be disc-shaped, and has a first surface 11 and a second surface 12 that are arranged oppositely. Specifically, if the first surface 11 is called the front side, the second surface 12 is for the back. It should be noted that due to the viewing angle presented in FIG. 2, the second surface 12 is marked on the edge of the second surface 12, and it refers to the back side of the flow channel body 1 (second surface 12), which is hereby described . Structurally, the channel body 1 includes a sample injection port 13 , at least one sample channel 14 , at least one separation tank 15 , a reagent injection port 16 , at least one reagent channel 17 and at least one mixing channel 18 . It should be noted that the present invention does not limit the number of sample channels 14, separation tanks 15, reagent channels 17, mixing channels 18 and collection units 2. In this embodiment, one sample channel 14, one separation tank 15, A reagent channel 17 , a mixing channel 18 and a collection unit 2 are described as examples. In other embodiments, there may be multiple sample flow channels 14 , separation tanks 15 , reagent flow channels 17 , mixing flow channels 18 and collection units 2 , and the present invention is not limited thereto.

The channel body 1 of this embodiment is disc-shaped, that is, a disc-shaped disc with a thickness, so that the sample channel 14, the separation tank 15, the reagent channel 17 and the mixing channel 18 can be formed inside the channel body 1, The sample injection port 13 and the reagent injection port 16 are located on the first surface 11 of the channel body 1 . And the material of the runner body 1 can be plastic material, such as polymethyl methacrylate (PMMA), polycarbonate (PC) or other thermoplastics. The diameter of the runner body 1 can be between 6cm and 18cm, preferably 12cm.

Specifically, the sample injection port 13 is disposed on the first surface 11 , that is, the sample injection port 13 has an opening structure on the first surface 11 , and the sample channel 14 is formed inside the flow channel body 1 and connected to the sample injection port 13 . Preferably, the sample injection port 13 is located at the geometric center of the flow channel body 1, which is more conducive to the continuous injection of blood samples during the operation of the centrifugal flow channel device C1. It should be noted that the geometric center mentioned in this embodiment is not a center point, but an area adjacent to the geometric center. Specifically, the centrifugal flow channel device C1 of this embodiment can be used in conjunction with the rotating platform, and the centrifugal flow channel device C1 is driven to rotate by the rotating platform to generate the centrifugal force required to separate the contents of the sample. The centrifugal flow channel device C1 further has a through positioning hole O, and this embodiment takes three positioning holes O as an example. The centrifugal flow channel device C1 can be fixed on the rotating platform through the positioning hole O, and when the centrifugal flow channel device C1 is driven by the rotating platform to rotate, since the position of the geometric center of the flow channel body 1 remains unchanged, it will not be affected by the rotation. Therefore, the specimen sample can be continuously injected into the sample injection port 13 , so that it can be applied to experiments or detection methods that need to separate a large number of specimen samples. However, the present invention does not limit the configuration of the flow channel body 1 , it only needs to be able to rotate stably to generate centrifugal force, and preferably, the flow channel body 1 can be in the shape of a circular disk.

Similarly, the reagent injection port 16 is configured on the first surface 11, that is, the reagent injection port 16 has an opening structure on the first surface 11, and the reagent flow channel 17 is also arranged inside the flow channel body 1 and connected to the reagent injection port. 16. Preferably, the reagent injection port 16 can also be located at the geometric center of the flow channel body 1 , that is, an area adjacent to the geometric center. In this embodiment, the sample injection port 13 and the reagent injection port 16 are of the same shape but different in size, so that the sample injection port 13 and the reagent injection port 16 can be set at the geometric center of the flow channel body 1 at the same time, and the sample injection port 13 is in the The inside and the reagent injection port 16 are on the outside. Preferably, both the sample injection port 13 and the reagent injection port 16 are circular openings, and are concentrically arranged at the geometric center of the channel body 1 . Therefore, in addition to the continuous injection of the specimen sample, the continuous injection of reagents is also possible. In operation, when the centrifugal flow channel device C1 is running, a specimen sample, such as a blood sample, can be injected into the sample injection port 13, and a reagent can be injected into the reagent injection port 16, so that the blood sample and the reagent can pass through the centrifugal force. , and loaded into the sample channel 14 and the reagent channel 17 respectively.

One end of the sample flow channel 14 in this embodiment is connected to the sample injection port 13 arranged at the geometric center of the flow channel body 1 , and the other end extends outward in a spiral shape to form an arc-shaped structure surrounding the sample injection port 13 . And it is arranged inside the runner body 1 . The interior of the flow channel body 1 of this embodiment also has a separation groove 15 , and the separation groove 15 is adjacent to the sample flow channel 14 and communicates with the sample flow channel 14 . The separation groove 15 can also be the same as the sample channel 14 , presenting an arc structure surrounding the sample injection port 13 . In this embodiment, the separation groove 15 can be formed by partially extending the sample flow channel 14 outward, so the middle area of the sample flow channel 14 and the separation groove 15 form a state of being in communication with each other. Specifically, the overall structure of the sample flow channel 14 and the separation groove 15 presents a non-uniform width that is narrow at both ends and wide in the middle area, that is, it is narrow near the sample injection port 13 and the mixing flow channel 18, and the middle area is narrow due to The structure of the separation groove 15 presents a wider aspect. The cells in the blood sample can be screened out in the sample flow channel 14 and the separation tank 15 and the cells with lighter weight will be described in detail later.

Similarly, the reagent channel 17 can also present an arc structure around the reagent injection port 16 . One end of the mixing flow channel 18 in this embodiment further has a confluence point 181 to connect the sample flow channel 14 and the reagent flow channel 17, so that the separated blood sample and reagent can enter the mixed flow after converging at the confluence point 181. Road 18. The other end of the mixing flow channel 18 communicates with the collection unit 2 so that the mixed solution of the blood sample and the reagent can flow to the collection unit 2. Similarly, other details will be described in detail later.

The separation tank 15 of this embodiment is used to accommodate the density gradient solution. Since this embodiment is an example of separating circulating tumor cells in blood samples, the density gradient solution can be, for example but not limited to, Ficoll-Paque solution (Ficoll-paqueTMplusGEHealthcare ). The density gradient solution needs to be injected into the separation tank 15 before injecting the blood sample. While the flow channel body 1 is driven to rotate, the density gradient solution can form a density gradient in the separation tank 15, and the cells of different weights in the blood sample can be screened through the formation of the density gradient.

Fig. 3 is a schematic view of the flow channel of the centrifugal flow channel device shown in Fig. 1. The schematic view of the flow channel drawn in Fig. 3 is used here to illustrate the separation of circulating tumor cells in blood samples. Please refer to Fig. 1 and Fig. 3 at the same time shown. First, the density gradient solution forms a density gradient in the separation tank 15 through the action of centrifugal force, and the closer to the inner edge of the flow channel body 1, the lower the density, which is called low-density area A in this embodiment, and the closer to the flow channel body 1, the density is lower. The outer edge of the body 1 has a higher density, which is called a high-density area B in this embodiment. It should be noted that the density gradient from the inner edge to the outer edge of the separation groove 15 is continuous from low to high, while FIG. 3 is directly divided into low-density area A and high-density area B for the sake of clarity. In addition, roughly speaking, the blood sample contains heavy cells (Heavy cells) and light cells (Light cells), which are respectively referred to as large cell HC and small cell LC in this embodiment, wherein the large cell HC is, for example, Red blood cells, white blood cells, etc., and small cell LC such as circulating tumor cells, etc. When the flow channel body 1 is driven to rotate, the centrifugal force can drive the blood sample to flow from the sample injection port 13 to the outside of the flow channel body 1 (the flow direction is shown by the arrow), and can also drive the reagent from the reagent injection port 16 to the outside of the flow channel body 1. The flow flows in the outer direction of the flow channel body 1 and flows along the spiral (or arc) sample flow channel 14 and the reagent flow channel 17 respectively.

In the sample flow channel 14, when the blood sample flows through the junction of the sample flow channel 14 and the separation tank 15, the small cells LC or small molecules are suspended in the low-density area A under the action of centrifugal force and the screening of the density gradient solution. Driven by the centrifugal force, it further flows to the mixing flow channel 18, while the large cells HC or macromolecules are washed and settled in the high-density area B. In short, the flow path of the blood sample starts from the sample injection port 13 and flows along the sample flow channel 14, in which the small cells LC flow to the mixing channel 18 under the action of centrifugal force, while the large cells HC are simultaneously subjected to centrifugal force and density. Gradient screening and stay in the high-density area B of the separation tank 15, and then separate the small cell LC from the large cell HC, so that the blood sample entering the mixing flow channel 18 only contains small cell LC (including circulating tumor cells) and other small cells. molecular. The separated blood sample flows through the mixing flow channel 18 and then enters the collection unit 2, thereby achieving the effect of separating and collecting the circulating tumor cells in the blood sample.

In addition, in the reagent flow channel 17, after the reagent is injected into the reagent injection port 16, the reagent can flow through the reagent flow channel 17 and enter the mixing channel 18 through the action of centrifugal force, and then mixed with the separated blood sample. mix. Wherein, the reagent can be a buffer solution, or there are materials in the reagent that can label circulating tumor cells (ie detection targets), and the materials that can label circulating tumor cells can be, for example but not limited to, fluorescent dyes (fluorescent dyes), antibodies (antibody) , immune markers (immuno-marker), or magnetic beads (magneticbead), etc. Therefore, the circulating tumor cells can enter the collection unit 2 after being marked in the mixing flow channel 18, and the circulating tumor cells may also be marked in the collection unit 2, thereby achieving the effect of detecting the content of circulating tumor cells in the blood sample.

Figure 4 is an enlarged schematic view of the collection unit shown in Figure 2 and part of the mixing channel, as shown in Figure 4, preferably, the mixing channel 18 can have a plurality of microstructures 182, and these microstructures 182 are discontinuous It is installed inside the mixing channel 18. The arrangement of the microstructure 182 can increase the mixing degree of the reagent and the separated blood sample, so that the circulating tumor cells can be labeled reliably. It should be noted that, in order to keep the drawing simple, the microstructure 182 is only drawn in the enlarged view.

Please refer to Fig. 2 and Fig. 4 at the same time, the collection unit 2 of the present embodiment has an opening 21, at least one overflow hole 22 and an accommodating space 23, the opening 21 communicates with the mixing channel 18, and the waste liquid tank 3 communicates with the overflow hole 22 of the collection unit 2 , and the accommodating space 23 is disposed between the opening 21 and the overflow hole 22 . In this embodiment, the collection unit 2 is arranged on the outer periphery of the circular plate-shaped flow channel body 1, and the collection unit 2 can be directly formed inside the flow channel body 1, and the overflow hole 22 of the collection unit 2 Located on the second surface 12 of the runner body 1 . Preferably, the waste liquid tank 3 of this embodiment is an annular groove, and is connected to the second surface 12 of the flow channel body 1, that is, it is arranged below the flow channel body 1, and the waste liquid tank 3 can be formed directly on the flow channel body 1. The second surface 12 of the channel body 1 is detachably disposed on the channel body 1 , which is not limited by the present invention. Therefore, the opening 21 of the collection unit 2 can receive the separated and mixed blood sample from the mixing flow channel 18, and accommodate it in the accommodating space 23, and the user can directly move the centrifugal flow channel device C1 to the A device for observation to observe a separated and pooled blood sample (containing labeled circulating tumor cells). Also, since the content of circulating tumor cells in the blood is scarce, a large amount of blood samples need to be injected when performing such experiments, and when the collection unit 2 collects too many blood samples, the excessive blood samples can be discharged through the overflow hole 22. Blood samples were excluded. In addition, excessive blood samples can be received through the waste liquid tank 3 communicating with the overflow hole 22, so as to avoid the pollution of blood samples sprayed around the surroundings caused by the direct discharge of excessive blood samples, so the centrifugal flow channel device can be maintained Overall cleaning of C1 and the experimental platform used with it.

Preferably, as shown in FIG. 2, the central part of the structure of the waste liquid tank 3 further includes a top surface 31, and the top surface 31 is provided with a convex portion 32, and the location of the convex portion 32 corresponds to the position of the flow channel body 1. Sample injection port 13, sample channel 14, separation tank 15, reagent channel 17, mixing channel 18 and other structures. In addition, the protrusion 32 can be, for example but not limited to, an O-ring (O-ring). When the flow channel body 1 is combined with the waste liquid tank 3, the design of the protrusion 32 can prevent the liquid in the flow channel from overflowing. It has leak-proof effect.

In this embodiment, the flow channel body 1 , the collection unit 2 and the waste liquid tank 3 can have many different implementation styles, which will be further described in different embodiments below.

FIG. 5 is a schematic diagram of a centrifugal channel device C2 according to the second embodiment of the present invention, please refer to FIG. 5 . In this embodiment, the collecting unit 2a can also be detachably arranged on the channel body 1a. Wherein, the flow channel body 1a has a cavity or an opening. The present embodiment takes the opening 10a as an example, so that the collecting unit 2a is detachably arranged in the opening 10a (or cavity), and the length of the opening 10a of the present embodiment is The axial direction is parallel to the long axis direction of the flow channel body 1a, so that the collection unit 2a can be placed on the flow channel body 1a in a manner of sliding in sideways. The collection unit 2a is designed to be detachable, so that the user can only take out the collection unit 2a for observation. As for the detailed features of other elements of the centrifugal flow channel device C2 , you can directly refer to the content recorded in the centrifugal flow channel device C1 of the first embodiment, and will not be repeated here.

In addition, FIG. 6 is a schematic diagram of a centrifugal flow channel device C3 according to the third embodiment of the present invention. Please refer to FIG. 6, the long axis direction of the opening 10b of this embodiment is perpendicular to the long axis direction of the flow channel body 1a , so that the collection unit 2a can be placed directly on the opening 10b from above the flow channel body 1b. As for the detailed features of other elements of the centrifugal flow channel device C3 , you can directly refer to the content recorded in the centrifugal flow channel device C1 of the first embodiment, and will not be repeated here.

The collection units 2 (2a, 2b) of the first, second and third embodiments are arranged on the flow channel body 1 (1a, 1b) in a horizontal manner. In other embodiments, they can also be arranged on the flow channel body in a vertical manner. Runner body.

FIG. 7 is a schematic diagram of a centrifugal flow channel device C4 according to the fourth embodiment of the present invention. As shown in FIG. 7 , the collection unit 2c of this embodiment is vertically arranged on the flow channel body 1c. The opening 21c of the collection unit 2c of this embodiment is set on the upper edge of the collection unit 2c, the overflow hole 22c is set on the lower edge of the collection unit 2c, and the flow channel body 1c has an opening 10c correspondingly, and the opening 10c runs through the Tao ontology 1c. Therefore, when the collection unit 2c is accommodated in the flow channel body 1c, the opening 21c located on the upper edge of the collection unit 2c can communicate with the mixing flow channel 18c, and the overflow hole 22c can directly correspond to the waste liquid tank 3c, so that the waste liquid Tank 3c can accept excess blood samples. As for the detailed features of other components of the centrifugal flow channel device C4 , you can directly refer to the content recorded in the centrifugal flow channel device C1 of the first embodiment, and will not be repeated here.

Fig. 8A is a schematic diagram of a centrifugal flow channel device C5 according to the fifth embodiment of the present invention, Fig. 8B is an enlarged schematic diagram of the collection unit and part of the waste liquid tank shown in Fig. 8A, and Fig. 8C is a schematic diagram of the collection unit and part of the waste liquid tank shown in Fig. 8B Schematic diagram of waste tank disassembly. Please refer to FIG. 8A to FIG. 8C at the same time, the waste liquid tank 3d of this embodiment is arranged around the outside of the flow channel body 1d, and the outside of the flow channel body 1d in this embodiment refers to the outside of the side wall of the flow channel body 1d , as shown in Figure 8B and Figure 8C. The overflow hole 22d of the collection unit 2d in this embodiment is located outside the flow channel body 1d, that is, it is set on the side wall of the flow channel body 1d, and the inner side wall 33d of the waste liquid tank 3d has an opening corresponding to the overflow hole 22d H, connect the waste liquid tank 3d to the outside of the flow channel body 1d, the waste liquid tank 3d can be directly formed on the outside of the flow channel body 1c, or be detachably arranged on the outside of the flow channel body 1d, the present invention is not limited . Therefore, the overflow hole 22d can communicate with the waste liquid tank 3d, and the waste liquid tank 3d can also receive excess blood samples. Similarly, for details of other elements of the centrifugal flow channel device C5 , reference may be made directly to the content recorded in the centrifugal flow channel device C1 of the first embodiment, and details are not repeated here.

FIG. 9A is a schematic diagram of a centrifugal channel device C6 according to the sixth embodiment of the present invention, and FIG. 9B is a side view of the centrifugal channel device shown in FIG. 9A . Please refer to FIG. 9A and FIG. 9B at the same time. The flow channel body 1e of this embodiment may further have two columns 19e, which are respectively disposed on the periphery of the sample injection port 13e and the reagent injection port 16e. Specifically, in this embodiment, the peripheral edges of the sample injection port 13e and the reagent injection port 16e protrude upwards to form a retaining wall, and the retaining wall is called a column 19e in this embodiment. In this embodiment, the configuration of the column 19e can avoid the contamination of the blood sample mixed with the reagent before entering the sample channel 14e. Details and features of other elements of the centrifugal flow channel device C6 can be directly referred to the content recorded in the centrifugal flow channel device C1 of the first embodiment, and will not be repeated here.

10A is a schematic diagram of a centrifugal channel device according to a seventh embodiment of the present invention, and FIG. 10B is a top view of the centrifugal channel device shown in FIG. 10A . Please refer to FIG. 10A and FIG. 10B . In this embodiment, the sample injection port 13f, the sample flow channel 14f, the separation tank 15f, the reagent injection port 16f, the reagent flow channel 17f and the mixing channel 18f are all set in the flow channel body 1f, and the collection unit 2f is also set in the flow channel body The outer peripheral edge of 1f, and the waste liquid tank 3f is detachably arranged on the flow channel body 1f. In this embodiment, the flow channel body 1f is formed by stacking two disc-shaped plastic sheets, wherein the upper layer is called the first flow channel body M1 and the lower layer is called the second flow channel body M2. The above-mentioned flow channels and the injection ports can be arranged on the first flow channel body M1, and the lower plastic sheet is directly covered on the bottom side of the second flow channel body M2, as shown in FIG. 11A.

11A is a cross-sectional view of the centrifugal flow channel device shown in FIG. 10A along line A-A, and FIG. 11B is an exploded schematic view of the centrifugal flow channel device shown in FIG. 11A . Please refer to FIG. 11A and FIG. 11B at the same time. There are at least two hooks M11 and M21 on the second surface 12f of the flow channel body 1f, that is, the hook M11 is formed on the bottom side of the first flow channel body M1, and the hook M21 is formed on the bottom of the second flow channel body M2. side. The waste liquid tank 3f has an inner side wall 33f and an outer side wall 34f. The top edge of the inner side wall 33f and the top edge of the outer side wall 34f each have at least one locking groove 331f, 341f, and the hook M11 is engaged in the locking groove 341f, and The hook M21 is engaged with the engaging groove 331f, so that the waste liquid tank 3f is detachably disposed on the flow channel body 1f in an engaging manner. Therefore, waste liquid tanks 3f with different volumes can be selected according to the volume of the injected blood sample.

As shown in Figure 10B, the flow channel body 1f of this embodiment has a plurality of hooks M11 (or hooks M21) arranged at intervals, and between two adjacent hooks M11 (or two adjacent hooks M21) It further has a convex portion M12 (or a convex portion M22). In other words, the second surface 12f of the flow channel body 1f has at least two protrusions M12, M22 extending from the hooks M11, M21 to two sides, so that the protrusions M12, M22 and the hooks M11, M21 surround the flow channel together. The outer periphery of the body 1f. 12A is a cross-sectional view of the centrifugal flow channel device shown in FIG. 10A on the B-B section line, and FIG. 12B is an exploded schematic diagram of the centrifugal flow channel device shown in FIG. 12A . Please refer to FIG. 12A and FIG. 12B at the same time. The protrusions M12, M22 are also arranged in the slots 331f, 341f of the waste liquid tank 3f, please also match it as shown in FIG. , 341f. The configuration of the engaging grooves 331f, 341f can also be changed according to the configuration of the engaging hooks M11, M21 or the protrusions M12, M22. For example, the engaging grooves 331f, 341f can have gaps corresponding to the positions of the engaging hooks M11, M21 The hook portions of the hooks M11 and M21 are accommodated (as shown in FIG. 11A ); while the positions corresponding to the protrusions M12 and M22 do not need a notch structure.

Please refer to FIG. 11A and FIG. 11B , the flow channel body 1f further has two positioning holes O. In this embodiment, O-rings are used as an example, which are respectively arranged in the slots 331f and 341f, and then sandwiched by the hook M11. , M21 and the slots 331f, 341f, and interposed between the convex parts M12, M22 and the slots 331f, 341f (as shown in Figure 12A), so that the flow channel body 1f and the waste liquid tank 3f can be more closely Combined to achieve leak-proof effect.

In addition, the present invention further provides a centrifugal flow channel device, which includes a flow channel body, at least one collection unit and a waste liquid tank. The flow channel body has a first surface and a second surface which are arranged oppositely, and the flow channel body includes a sample flow channel, a reagent flow channel, and a mixing flow channel, wherein the sample injection port is arranged inside the flow channel body, and the sample flow The channel forms a sample injection port on the first surface, and part of the sample flow channel extends outward to form a separation groove. The reagent channel is arranged inside the channel body, and the reagent channel forms a reagent injection port on the first surface. The connection relationship between the sample injection port, the separation tank, the reagent injection port and other components is the same as that of the first embodiment, so the flow channel body 1 of the first embodiment can be directly referred to. And when the overflow hole of the collection unit is located on the second surface, the waste liquid tank is connected to the second surface of the flow channel body (as in the first embodiment), and when the overflow hole of the collection unit is located outside the flow channel body, and The waste liquid tank is connected to the outside of the flow channel body, as in the fourth embodiment, so the corresponding relationship between the collection unit and the waste liquid tank can directly refer to the centrifugal flow channel device C4 of the fourth embodiment, and will not be repeated here.

The present invention further provides a centrifugal flow channel device, which includes a flow channel body, a waste liquid tank and at least a collection unit. The flow channel body has a first surface and a second surface oppositely arranged, and the flow channel body includes a sample injection port, at least one sample flow channel, a separation tank, a reagent injection port, at least one reagent flow channel, and at least one mixed flow Road and at least one first connecting part. The sample injection port is arranged on the first surface. The sample flow channel is connected to the sample injection port. The separation groove is adjacent to the sample flow channel and communicated with the sample flow channel. The reagent injection port is configured on the first surface. The reagent flow channel is connected to the reagent injection port. One end of the mixing channel is connected to the sample channel and the reagent channel. The first connecting portion is disposed on the second surface. The waste liquid tank is detachably arranged on the flow channel body, and the waste liquid tank has at least one second connecting part, and the first connecting part and the second connecting part cooperate with each other. The collecting unit has an opening and at least one overflow hole, the opening communicates with the other end of the mixing channel, and the overflow communicates with the waste liquid tank.

Wherein, the sample injection port, the sample flow channel, the separation tank, the reagent injection port, the reagent flow channel, and the mixing channel of the flow channel body can refer to the foregoing embodiments, and the second surface of the flow channel body in this embodiment is further provided with The first connection part and the waste liquid tank have a corresponding second connection part, and the first connection part and the second connection part cooperate with each other, for example, the first connection part can be a hook, and the second connection part can be a slot. Therefore, the first connection part and the second connection part can refer to the centrifugal flow channel device C7 of the seventh embodiment mentioned above, and the other components, and their detailed connection relationship and changes can refer to the previous embodiment, which will not be described here. Let me repeat.

In summary, according to the centrifugal flow channel device of the present invention, through the design of the flow channel, especially the configuration relationship between the sample flow channel and the separation tank, when the specimen sample flows through the junction of the sample flow channel and the separation tank , through the action of centrifugal force and the screening of the density gradient solution, the lighter cells or molecules are driven by centrifugal force to flow to the mixing channel, while the heavier cells or molecules are washed and settled in the separation tank, and then Achieve the effect of separating substances in the specimen sample.

In addition, the waste liquid tank is designed to communicate with the overflow hole of the collection unit, so that the waste liquid tank can accept too many specimen samples, and then can easily process a large number of specimen samples, and at the same time maintain the centrifugal flow channel device as a whole cleaning to avoid contamination.

The above descriptions are illustrative only, not restrictive. Any equivalent modification or change made without departing from the spirit and scope of the present invention shall be included in the scope of the appended patent application.

Claims (20)

1. A centrifugal flow channel device, characterized in that, comprising: The flow channel body has a first surface and a second surface oppositely arranged, and the flow channel body includes: a sample injection port configured on the first surface; At least one sample flow channel is connected to the sample injection port; At least one separation groove is located adjacent to the sample flow channel and communicates with the sample flow channel; a reagent injection port configured on the first surface; at least one reagent flow channel connected to the reagent injection port; and at least one mixing flow channel, one end of which is connected to the sample flow channel and the reagent flow channel; At least one collection unit has an opening and at least one overflow hole, the opening communicates with the other end of the mixing channel; and A waste liquid tank communicates with the overflow hole of the collection unit. 2 . The centrifugal flow channel device according to claim 1 , wherein the overflow hole of the collection unit is located on the second surface, and the waste liquid tank is connected to the second surface of the flow channel body. 3 . 3 . The centrifugal flow channel device according to claim 1 , wherein the overflow hole of the collecting unit is located outside the flow channel body, and the waste liquid tank is connected to the outside of the flow channel body. 4 . 4. The centrifugal flow channel device according to claim 1, wherein the waste liquid tank is detachably disposed on the flow channel body. 5. The centrifugal flow channel device according to claim 4, wherein the waste liquid tank has an inner side wall and an outer side wall, the second surface of the flow channel body has at least two hooks, and the inner side wall The top edge of the outer wall and the top edge of the outer wall each have at least one slot, and the hook is engaged with the slot. 6. The centrifugal flow channel device according to claim 5, wherein the second surface of the flow channel body has at least two protrusions extending from the hook to two sides to surround the flow channel On the outer peripheral edge of the body, the hook and the protrusion are arranged in the slot of the waste liquid tank. 7 . The centrifugal flow channel device according to claim 5 , wherein the flow channel body further has two elastic members disposed in the two engaging grooves. 8 . 8. The centrifugal flow channel device according to claim 1, wherein the collection unit is detachably disposed on the flow channel body. 9 . The centrifugal flow channel device according to claim 8 , wherein the flow channel body has a cavity or an opening, and the collecting unit is disposed in the cavity or the opening. 10 . The centrifugal flow channel device according to claim 1 , wherein the flow channel body has two columns, which are respectively arranged on the periphery of the sample injection port and the reagent injection port. 11 . 11. The centrifugal flow channel device according to claim 1, wherein the flow channel body is in the shape of a circular disk, the waste liquid tank is an annular groove, and is arranged on the second surface of the flow channel body , or surround the outside of the flow channel body. 12 . The centrifugal flow channel device according to claim 1 , wherein the sample flow channel partially extends outward to form the separation groove. 13 . 13 . The centrifugal flow channel device according to claim 1 , wherein the sample injection port is disposed at the geometric center of the flow channel body, and the sample flow channel extends outward in a helical shape. 14 . 14. A centrifugal flow channel device, characterized in that it comprises: The flow channel body has a first surface and a second surface oppositely arranged, and the flow channel body includes: At least one sample flow channel is arranged inside the flow channel body, the sample flow channel forms a sample injection port on the first surface, and a part of the sample flow channel extends outward to form a separation groove; At least one reagent flow channel is arranged inside the flow channel body, and the reagent flow channel forms a reagent injection port on the first surface; and at least one mixing flow channel, one end of which is connected to the sample flow channel and the reagent flow channel; At least one collection unit has an opening and at least one overflow hole, the opening communicates with the other end of the mixing channel; and A waste liquid tank communicates with the overflow hole of the collection unit. 15. A centrifugal channel device, characterized in that it comprises: The flow channel body has a first surface and a second surface oppositely arranged, and the flow channel body includes: a sample injection port configured on the first surface; At least one sample flow channel is connected to the sample injection port; At least one separation groove is located adjacent to the sample flow channel and communicates with the sample flow channel; a reagent injection port configured on the first surface; at least one reagent flow channel connected to the reagent injection port; at least one mixing flow channel, one end of which is connected to the sample flow channel and the reagent flow channel, and the other end communicates with the opening of the collection unit; and at least one first connecting portion is disposed on the second surface; a waste liquid tank, which is detachably arranged on the flow channel body, the waste liquid tank has at least one second connecting part, and the first connecting part cooperates with the second connecting part; and At least one collecting unit has an opening and at least one overflow hole, the opening communicates with the other end of the mixing channel, and the overflow communicates with the waste liquid tank. 16. The centrifugal flow channel device according to claim 15, wherein the first connecting portion is a hook, and the second connecting portion is a groove. 17. The centrifugal flow channel device according to claim 16, wherein the waste liquid tank has an inner side wall and an outer side wall, the second surface of the flow channel body has at least two hooks, the inner side The top edge of the wall and the top edge of the outer wall each have at least one engaging groove, and the two engaging hooks are engaged in the two engaging grooves. 18. The centrifugal flow channel device according to claim 17, wherein the second surface of the flow channel body has at least two protrusions extending from the hook to two sides to surround the flow channel On the outer peripheral edge of the main body, the two hooks and the two protrusions are jointly arranged in the two locking grooves of the waste liquid tank. 19 . The centrifugal flow channel device according to claim 16 , wherein the flow channel body further has two elastic members disposed in the two engaging grooves. 19 . 20. The centrifugal flow channel device according to claim 15, wherein the flow channel body is in the shape of a circular disk, and the waste liquid tank is an annular groove, which is arranged on the second surface of the flow channel body , or surround the outside of the flow channel body.