CN1056895A - A kind of high adhesive biological reactor - Google Patents
A kind of high adhesive biological reactor Download PDFInfo
- Publication number
- CN1056895A CN1056895A CN 90105299 CN90105299A CN1056895A CN 1056895 A CN1056895 A CN 1056895A CN 90105299 CN90105299 CN 90105299 CN 90105299 A CN90105299 A CN 90105299A CN 1056895 A CN1056895 A CN 1056895A
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- CN
- China
- Prior art keywords
- retort
- biological reactor
- high adhesive
- static mixer
- adhesive biological
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000000853 adhesive Substances 0.000 title claims abstract description 19
- 230000001070 adhesive effect Effects 0.000 title claims abstract description 19
- 230000003068 static effect Effects 0.000 claims abstract description 33
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 238000000855 fermentation Methods 0.000 abstract description 16
- 230000004151 fermentation Effects 0.000 abstract description 16
- 239000007789 gas Substances 0.000 abstract description 10
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 8
- 239000001301 oxygen Substances 0.000 abstract description 8
- 229910052760 oxygen Inorganic materials 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 7
- 239000007788 liquid Substances 0.000 abstract description 6
- 230000000813 microbial effect Effects 0.000 abstract description 6
- 238000012546 transfer Methods 0.000 abstract description 3
- 241000894006 Bacteria Species 0.000 abstract description 2
- 238000005265 energy consumption Methods 0.000 abstract description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 abstract description 2
- 210000000056 organ Anatomy 0.000 description 6
- 229920001285 xanthan gum Polymers 0.000 description 4
- 229940082509 xanthan gum Drugs 0.000 description 3
- 235000010493 xanthan gum Nutrition 0.000 description 3
- 239000000230 xanthan gum Substances 0.000 description 3
- KFSLWBXXFJQRDL-UHFFFAOYSA-N Peracetic acid Chemical compound CC(=O)OO KFSLWBXXFJQRDL-UHFFFAOYSA-N 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 238000006713 insertion reaction Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- GJCOSYZMQJWQCA-UHFFFAOYSA-N 9H-xanthene Chemical compound C1=CC=C2CC3=CC=CC=C3OC2=C1 GJCOSYZMQJWQCA-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 244000043261 Hevea brasiliensis Species 0.000 description 1
- 239000003082 abrasive agent Substances 0.000 description 1
- 230000036983 biotransformation Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000002826 coolant Substances 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000012262 fermentative production Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 229910002804 graphite Inorganic materials 0.000 description 1
- 239000010439 graphite Substances 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229920003052 natural elastomer Polymers 0.000 description 1
- 229920001194 natural rubber Polymers 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention belongs to microbial fermentation engineering.
In the microbial fermentation field, the high viscosity microbial fermentation is a difficult problem at present, the objective of the invention is to design a kind of novel high adhesive biological reactor in order to solve this difficult problem of high viscosity microbial fermentation.Main points of the present invention are that this high adhesive biological reactor has an external circulating device of being made up of static mixer (3), spiral pump (5), circulation tube (6), and a gas block panel (3) is arranged at the bottom in retort (7).It mixes with " static state " and replaces dynamically mixing, and has solved gas in the high viscosity flow container-liquid mixed problem preferably.Advantages such as it is simple in structure, easily removable and washable, easy to operate that the present invention has, and improved the oxygen transfer efficiency, reduced energy consumption, improved fermentation productivity, bacterium there is not any physiology disadvantageous effect.
Description
The invention belongs to microbial fermentation engineering
Microbial polysaccharide is the outer water-soluble glue of born of the same parents that is produced by the certain micro-organisms fermentation.Especially xanthan gum has good performance and than more comprehensive function, of many uses, market outlook are wide.But xanthan gum viscosity is high, and the viscosity variation can reach 2000-10000cp during fermentation, and this just has higher requirement to reactor.Gather at present the traditional mechanical agitator tank of the most of employing of polyose high-viscosity material fermentative production both at home and abroad, owing to adopt its mixed performance of turbine type agitator very poor.Improved in recent years a mixing bowl mainly is to make agitator into helical-ribbon type, and is not obvious though the oxygen transmission effect has improvement, and shortcoming is that the microcosmic shear effect is bad.Some scholar also studied with air lift type and replaced stirred pot to carry out high viscosity fermentation the present age, because gas consumption is very big, mixed effect does not improve to some extent than stirred pot, the air lift type stirred reactor is not suitable for producing high-viscosity material, because running into high viscosity liquid, the air-flow of single tube ejection is difficult to form small bubbles, in the international bio-reactor meeting of holding in Scotland, the A.Mermann of West Germany University of Munich proposes a kind of latest model high adhesive biological reactor.(seeing G.W.Moody:Bioreactors and Biotransformations Paye 287-296) is to utilize a rotary and centrifugal device dynamically to mix, and effect is pretty good, but because its complex structure, apart from a practical segment distance in addition.The development of high adhesive biological reactor is a global problem in the contemporary field of fermentation engineering at present.
Purpose of the present invention is exactly in order to solve the existing problem of above-mentioned several high adhesive biological reactor, and designs a kind of novel high adhesive biological reactor.
Main points of the present invention are: this high adhesive biological reactor of being made up of pump (5), retort (7), circulation tube (6), it is characterized in that this high adhesive biological reactor adopts one to form external circulating device by static mixer (3), pump (5), circulation tube (6), circulation tube (6) picks out from the bottom of retort (7), get back to retort (7) inside from the top of reaction cylinder (7) through pump (5), static mixer (3), and a gas block panel (9) is arranged at the bottom in reaction cylinder (7).Its structure is seen accompanying drawing 1.
Gas block panel (9) in the retort (7) is elected circle as, and diameter is 1/2 of retort (a 7) diameter, in retort (7) central interior, apart from retort (7) bottom 10cm, with support (10) it is connected on the inwall of retort (7).Its structure is seen accompanying drawing 1.
Static mixer (3) is contained in the static mixing organ pipe (2), and the two ends of static mixing organ pipe (2) are the baffle plates that has mesh.Static mixer (3) contains a plurality of static mixing unit, and each static mixing unit is the combination of two kinds of Kenics and Sulzer static mixer, but structure is different.In the Kenics static mixer, each blade Rotate 180 °, and in static mixer of the present invention (3), 360 ° of each blade rotations.Array mode is earlier in static mixing organ pipe (2) bottom, alternately arrange earlier overlapping 5 inclined corrugated plates, up connect two again and be twisted into 360 ° of blades of 90 ° that stagger each other, and then connect and alternately arrange overlapping 5 inclined corrugated plates, and the like, form a plurality of static mixing unit, the length-width-ratio of each rotating paddle is that the unitary length-width-ratio of each static mixing of L: D=2.5 is L: D=7.See accompanying drawing 2.
Circulation tube (6) is in the top insertion reaction cylinder (7) of retort (7), and the mouth of pipe of its circulation tube (6) should be placed on apart from 1/3-1/2 place, reaction cylinder (7) bottom, accompanying drawing 1.
The required pump of this high adhesive biological reactor can adopt end face axle envelope with adopting various ways wherein with spiral pump the best between spiral pump pump case and the axle.Pump stator is the synthetic stator of heatproof that forms with 1/4 silicon rubber glue and the compacting of 3/4 natural rubber, and stator surface is coated with the special high-abrasive material graphite body of one deck.Want sky to disappear during high adhesive biological reactor work, the two separates retort when sky disappears (7) and spiral pump (6), and retort (7) disappears with ordinary method saturation steam sky.Spiral pump (5) with millesimal Peracetic Acid sky disappear 30 minutes stand-by.Real the disappearing of charging disappears end in the time of preceding 5 minutes real in the retort (7), spiral pump (5) and reaction cylinder (7) join start spiral pump (5) running whole reactor system reality is disappeared, logical water coolant is to room temperature in chuck (8) after 5 minutes, and the sterile air pressurize is waited to inoculate.Gas-liquid becomes 0.5-0.9vvm during fermentation operation, and reaction cylinder (7) cycling time is that the 10-20 minute interior flow rate control of circulation tube (6) is at 2m/sec.
Usually general bio-reactor only satisfies the dissolved oxygen requirement when liquid viscosity is lower than 2000cp, when liquid viscosity can't satisfy the dissolved oxygen requirement when 2000cp is above.And the present invention mixes with " static state " and replaces dynamically mixing owing to adopted static mixer (3), spiral pump outside circulation structure, has more successfully solved solution-air mixed questions in the high viscosity fluid cylinder, can satisfy the dissolved oxygen requirement under the high viscosity.Improve the oxygen transfer efficiency and reduced energy consumption, and improved fermentation productivity, advantage such as this high adhesive biological reactor has simple in structure, and is easily removable and washable, easy to operate, and bacterium do not had any physiology disadvantageous effect; Owing to improved the oxygen transmission effect, the quality of metabolism product has also improved it and has stepped major step to this global problem that solves high viscosity fermentation.Be that one group of data compares below.
Four kinds of reactor dissolved oxygens of table 1 performance relatively
Reactor volume dissolved oxygen mass transfer coefficient KLa＜hr
-1
Type air flow polysaccharide＜Xanthan〉concentration
vvM
0 0.5% 1.0% 1.5% 2.0% 2.5%
A mixing bowl 1 133.8 102.2 75.5 60.0 46.2 15.5
Gas lift internal recycle 3 447.4 211.4 110.6 105.6 27.3 10.8
Gas lift outer circulation 3.7 350.3 116.6 70.6 33.0 10.3 9.8
The present invention 0.9 700.5 610.6 519.3 340.5 200.5 150.7
Three kinds of pattern reactors of table 2 xanthan gum fermentation result relatively
The air flow fermentation broth viscosity changes in time
Type VVM one day two days three days
A mixing bowl 1 985.0
Cp3100
Cp5400
Cp
Air lift type internal recycle 3.7 1100
Cp3000
Cp4400
Cp
The present invention 0.9 2600
Cp6500
Cp11000
Cp
Unit: cp
Most preferred embodiment 1:
Retort (7) diameter is got 50CM, height is 200CM, inner garden shape gas block panel (9) diameter of retort this moment (7) is 25CM, apart from the bottom is 10CM, circulation tube (6) diameter is that 2CM picks out by spiral pump (5) from retort (7) bottom, again by static mixing organ pipe (2), static mixing organ pipe (2) diameter is 5CM, and height is 200CM, and static mixer (3) has closed 5 static mixing unit, circulation tube (6) is from retort (7) top insertion reaction jar (7) inside, apart from retort (7) bottom 1/3, (about 67CM) locates, during reactor work, the interior flow velocity of circulation tube (6) is 2m/sec, and vapour-liquid ratio is 0.9VVM during fermentation.
Fig. 1. be high adhesive biological reactor structural representation (ratio 1: 20).1 is the air outlet among the figure, and 2 is the static mixing organ pipe, and 3 is static mixer, and 3a is a rotating paddle, and 3b is an inclined corrugated plate, and 4 is the sterile air import, and 5 is that spiral pump 6 is circulation tube, and 7 is retort, and 8 is chuck, and 9 is gas block panel, and 10 is gas block panel, and 11 is support.
Fig. 2 is a static mixing modular construction synoptic diagram.Among the figure, 3a is an inclined corrugated plate for rotating paddle 3b.
Fig. 3 is two inclined corrugated plates and overlapping form.
Claims (4)
1, a kind of high adhesive biological reactor of being made up of pump (5), retort (7), circulation tube (6) is characterized in that this high adhesive biological reactor has one by static mixer (3), pump (5).The external circulating device that circulation tube (7) is formed, circulation tube (6) picks out from the bottom of retort (7), through pump (5), static mixer (3), gets back to reaction cylinder (7) inside from the top of retort (7).A gas block panel (9) is arranged at the bottom in reaction cylinder (7).
2, high adhesive biological reactor according to claim 1, the garden that the is shaped as shape that it is characterized in that gas block panel (9), diameter are 1/2 of reaction cylinder (7) diameter, apart from 10cm place, retort (7) bottom, join by support (10) and reaction cylinder (7) inwall, see accompanying drawing 1.
3, high adhesive biological reactor according to claim 1 is characterized in that static mixer (3) contains several static mixing unit, and each static mixing unit all is the combination of oblique wave letter plate static mixer and rotating paddle static mixer.Its array mode is alternately to arrange earlier overlapping 5 inclined corrugated plates to connect two again and be twisted into 360 ° of blades of 90 ° that stagger each other, and its structure is seen accompanying drawing 2, and the length and width of each rotating paddle become L=D=2.5, the unitary long-width ratio L of each static mixing: D=7.
4, high adhesive biological reactor according to claim 1, it is characterized in that getting back to inner its mouth of pipe of circulation tube (6) of retort (7) should be apart from 1/3-1/2 place, retort (7) bottom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 90105299 CN1056895A (en) | 1990-05-30 | 1990-05-30 | A kind of high adhesive biological reactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 90105299 CN1056895A (en) | 1990-05-30 | 1990-05-30 | A kind of high adhesive biological reactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1056895A true CN1056895A (en) | 1991-12-11 |
Family
ID=4879126
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 90105299 Pending CN1056895A (en) | 1990-05-30 | 1990-05-30 | A kind of high adhesive biological reactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1056895A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1078247C (en) * | 1998-01-22 | 2002-01-23 | 中国科学院生态环境研究中心 | Bioreactor with baffling element |
| CN100488619C (en) * | 2000-12-28 | 2009-05-20 | 阿肯马法国公司 | Method and device for carrying out a gas-releasing reaction in a liquid medium |
| CN106010962A (en) * | 2016-06-17 | 2016-10-12 | 山东宝莫生物化工股份有限公司 | Catalytic hydration reactor and method for producing acrylamide through same |
| CN116987586A (en) * | 2023-07-28 | 2023-11-03 | 安及义实业（上海）有限公司 | Bioreactor and continuous flow reaction system |
-
1990
- 1990-05-30 CN CN 90105299 patent/CN1056895A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1078247C (en) * | 1998-01-22 | 2002-01-23 | 中国科学院生态环境研究中心 | Bioreactor with baffling element |
| CN100488619C (en) * | 2000-12-28 | 2009-05-20 | 阿肯马法国公司 | Method and device for carrying out a gas-releasing reaction in a liquid medium |
| CN106010962A (en) * | 2016-06-17 | 2016-10-12 | 山东宝莫生物化工股份有限公司 | Catalytic hydration reactor and method for producing acrylamide through same |
| CN116987586A (en) * | 2023-07-28 | 2023-11-03 | 安及义实业（上海）有限公司 | Bioreactor and continuous flow reaction system |
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| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
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