CN105409992A - Sterilization composite and application thereof - Google Patents

Abstract

The invention belongs to the field of agricultural fungicides, in particular to a fungicidal composition containing pyraclostrobin and picoxystrobin and an application thereof. The active ingredients of the fungicidal composition are pyraclostrobin and picoxystrobin, and the dosage forms are suspension concentrate and water dispersible granule, and the weight ratio of the two is 1:20～15:1, preferably 5:1～1: 8. Its advantages and beneficial effects are as follows: 1. Wide bactericidal range; 2. Long duration; 3. Low toxicity, no residue, no pollution, safe for the environment; When used, it is lowered, thereby improving the bactericidal effect, increasing safety and reducing environmental pollution.

Description

A kind of bactericidal composition and its application

technical field

The invention belongs to the field of agricultural fungicides, in particular to a fungicidal composition containing pyraclostrobin and picoxystrobin and an application thereof.

Background technique

Pyraclostrobin is a new type of broad-spectrum fungicide, and its mechanism of action is that it is a mitochondrial respiration inhibitor, that is, it has the functions of protection, treatment, and leaf penetration by preventing electron transfer during cytochrome synthesis. In addition to its strong inhibitory effect on fungi, pyraclostrobin can also significantly promote the growth of crops, which can significantly improve the growth of crops, improve immunity, and better defend against diseases. Pyraclostrobin is highly toxic to fish, but low toxic to birds, bees and earthworms. It should be indicated on the product label that medical equipment should not be washed in ponds and other water sources and water bodies, and that pesticide residues should not be poured into water sources and water bodies. Due to the unique mechanism of action of pyraclostrobin and the promotion of crop growth, it is also effective against bacteria resistant to other fungicides, so this agent has great room for development.

Picoxystrobin is a systemic fungicide with a broad spectrum of control objects. It is mainly used to control wheat foliar diseases such as leaf blight, leaf rust, glume blight, brown spot, powdery mildew, etc., and other methoxy Compared with acrylate-based fungicides, picoxystrobin has a stronger therapeutic effect on wheat leaf spot, net spot and moire. The mechanism of action is the same as the inhibitor of mitochondrial respiration, that is, the respiration of mitochondria is inhibited by electron transfer between cytochrome b and C1. It is effective in controlling strains resistant to 14-demethylase inhibitors, benzamides, tricarboxamides and benzimidazoles. Once taken up by the leaves, picoxystrobin moves in the xylem, following the water flow in the transport system; it also flows in the gas phase on the leaf surface and flows in the xylem after being absorbed into the leaves from the gas phase. A comparison of crops sprayed with picoxystrobin [250g(ai)/hm ² ] under no-rain conditions and crops exposed to 10mm of rainfall for 1h after the same spray treatment 2h showed both effects on barley The control effect of leaf blight is consistent. It is precisely because of the systemic activity and fumigation activity of picoxystrobin that the active ingredients can be effectively redistributed and fully delivered after application. Therefore, picoxystrobin has a better effect than commercial azoxystrobin and trifloxystrobin. Therapeutic activity.

Wheat powdery mildew, gramineous powdery mildew wheat specialization type; Scientific name: Blumeria graminis (DC.) Speer=Erysipe graminis DC., E. graminis DC.f.sp.triticl. Marchal is a worldwide disease, which is distributed in all major wheat-producing countries. It is common in Shandong coastal areas, Sichuan, Guizhou, and Yunnan, and the damage is also serious. In recent years, the disease has also become more and more serious in the Northeast, North China, and Northwest wheat regions. The disease can infect the above-ground organs of wheat plants, but mainly leaves and leaf sheaths, and glumes and awns can also be affected when the disease is severe.

Fungicide varieties containing a single active component often have different degrees of defects in the control of agricultural fungicides, such as narrow bactericidal range, poor control effect, large application doses are easy to produce drug resistance, and frequent application increases environmental pollution. One way to control agriculturally resistant pathogens is to introduce new ingredients that are not cross-resistant with existing varieties. However, the development cost of new active ingredients is high, the development cycle is long, and the speed of resistance of bacteria can never be kept up. Other methods, such as crop layout adjustment, rotation of different pesticides, etc., are difficult to achieve obvious effects in actual operation.

Combination of ingredients from different varieties is the most common method for controlling resistant pathogens. Compounding different ingredients can improve the control effect, expand the scope of sterilization, reduce the amount of active ingredients, save drug costs, and reduce environmental pollution. It is an important means of comprehensive disease prevention and control.

Contents of the invention

The object of the present invention is to provide a bactericidal composition containing pyraclostrobin and picoxystrobin with high bactericidal activity, long duration, high quick-acting, low toxicity and environmental protection and its application.

Technical scheme of the present invention is as follows:

A bactericidal composition, the active ingredients of which are pyraclostrobin and picoxystrobin, and the formulations are suspension concentrate and water dispersible granule.

The weight ratio of the active ingredient pyraclostrobin to picoxystrobin is 1:20-15:1, preferably 5:1-1:8.

The weight percentage of the active ingredients pyraclostrobin and picoxystrobin in the bactericidal composition is 10% to 80%.

The bactericidal composition also includes carriers and auxiliary agents.

The carrier is selected from one or more of water and fillers.

The auxiliary agent includes a surfactant, and the surfactant is selected from one or more of an emulsifying agent, a dispersing agent, a wetting agent or a penetrating agent.

The auxiliary agent also includes other functional auxiliary agents, and the other functional auxiliary agents are selected from one or more of antifreeze agents, thickeners, stabilizers, disintegrants or defoamers.

An application of the bactericidal composition in preventing and treating wheat powdery mildew.

The advantages and beneficial effects of the present invention: 1. wide range of sterilization; 2. long duration; 3. low toxicity, no residue, no pollution, safe for the environment; It is lower than when it is used alone, thereby improving the bactericidal effect, increasing safety and reducing environmental pollution.

detailed description

The present invention will be described in detail below in conjunction with the examples. However, the present invention is by no means limited to the following examples. The percentages in the formula are all percentages by weight, and the original drugs and auxiliary agents used are all purchased from other manufacturers.

Recipe screening is as follows:

The two active ingredients, pyraclostrobin and picoxystrobin, have high bactericidal activity. According to the characteristics of the two ingredients, the object of the indoor biotest is positioned as wheat powdery mildew. The specific test conditions and results are as follows:

The experimental drug used 95% pyraclostrobin and 95% picoxystrobin original drug, and used acetone to dissolve the original drug, and prepared the required preparation (according to the set ratio concentration, the required concentration of the drug solution was prepared for use) , the original drugs and additives used are purchased from other manufacturers).

The indoor bioassay method is as follows: the potted method is adopted, and the chemical agent is evenly sprayed on the spare wheat seedlings by spraying method, and then dried naturally. In the experiment, the treatment without drug was used as blank control. The fresh spores of powdery mildew fungus produced on the diseased wheat leaves within 24 hours were evenly shaken off and inoculated on the treated potted wheat seedlings at the 2-3 leaf stage. No less than 3 pots per treatment, 10 plants per pot. The protective test was inoculated 24 hours after the drug treatment; the curative test was inoculated 24 hours before the drug treatment, and then cultured under suitable conditions. According to the incidence of the blank control graded investigation. The following grading method was adopted: grade 0: no disease; grade 1: lesion area accounted for less than 5% of the entire area; grade 3: lesion area accounted for 6% to 15% of the entire area; 16% to 25% of the entire patch area; Grade 7: the lesion area accounts for 26% to 50% of the entire patch area; Grade 9: the lesion area accounts for more than 50% of the entire patch area.

According to the survey data, the disease index and control effect of each treatment were calculated.

The condition index is calculated according to the formula (1), and the calculation result has two decimal places:

$\mathrm{<span\; class="notranslate">\; x</span>}<span\; class="notranslate">\; =</span>\frac{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}<span\; class="notranslate">\; (</span>\mathrm{<span\; class="notranslate">\; N</span>}\mathrm{<span\; class="notranslate">\; i</span>}<span\; class="notranslate">\; \&times;</span>\mathrm{<span\; class="notranslate">\; i</span>}<span\; class="notranslate">\; )</span>}{\mathrm{<span\; class="notranslate">\; N</span>}<span\; class="notranslate">\; \&times;</span>\mathrm{<span\; class="notranslate">\; 9</span>}}<span\; class="notranslate">\; \&times;</span>\mathrm{<span\; class="notranslate">\; 100...</span>}<span\; class="notranslate">\; (</span>\mathrm{<span\; class="notranslate">\; 1</span>}<span\; class="notranslate">\; )</span>$

In the formula: x—sickness index; Ni—the number of diseased leaves at all levels; i—the value of relative grade; N—the total number of leaves under investigation.

The control effect is calculated according to the formula (2):

$\mathrm{<span\; class="notranslate">\; P</span>}<span\; class="notranslate">\; =</span>\frac{\mathrm{<span\; class="notranslate">\; C</span>}\mathrm{<span\; class="notranslate">\; K</span>}<span\; class="notranslate">\; -</span>\mathrm{<span\; class="notranslate">\; P</span>}\mathrm{<span\; class="notranslate">\; T</span>}}{\mathrm{<span\; class="notranslate">\; C</span>}\mathrm{<span\; class="notranslate">\; K</span>}}<span\; class="notranslate">\; \&times;</span>\mathrm{<span\; class="notranslate">\; 100...</span>}<span\; class="notranslate">\; (</span>\mathrm{<span\; class="notranslate">\; 2</span>}<span\; class="notranslate">\; )</span>$

In the formula: P—control effect, the unit is percentage (%); CK—blank control disease index; PT—pharmaceutical treatment disease index.

According to the logarithmic value of each agent concentration and the corresponding probability value of control effect, the regression analysis was carried out to calculate the EC50, EC90 equivalent value and 95% confidence limit of each agent. In the determination of drug joint toxicity, the co-toxicity coefficient was calculated according to Sun Yunpei's method.

The joint toxicity of the mixed preparation is expressed by Sun Yunpei's co-toxicity coefficient method:

The theoretical toxicity index TTI of the mixed preparation = Σ (toxicity index ATI of a certain drug × the percentage of the active ingredient of the drug in the mixed preparation).

When the CTC is greater than 120, it is a synergistic effect, when it is less than 80, it is an antagonistic effect, and when it is 80-120, it is an additive effect.

Table 1 Toxicity test results of different mixtures against wheat powdery mildew

The analysis results of the above data show that when pyraclostrobin and picoxystrobin are compounded in different proportions, the co-toxicity coefficients of all the compound formulas are respectively greater than 120, and all have obvious synergistic effects. It shows that after compounding the two active ingredients, it can have a good synergistic effect. Among them, the co-toxicity coefficient of ratio five even reached 176.27, the highest among all ratios.

The recipe example is as follows:

Example 1: 51% pyraclostrobin·picoxystrobin suspension concentrate

Example 2: 46.5% pyraclostrobin · picoxystrobin water dispersible granules

Example 3: 22% pyraclostrobin·picoxystrobin suspension concentrate

Example 4: 18% pyraclostrobin · picoxystrobin water dispersible granules

Example 5: 30% pyraclostrobin·picoxystrobin suspension concentrate

Example 6: 24% pyraclostrobin · picoxystrobin water dispersible granules

Example 7: 11% pyraclostrobin·picoxystrobin suspension concentrate

Example 8: 31% pyraclostrobin · picoxystrobin water dispersible granules

Example 9: 25.5% pyraclostrobin·picoxystrobin suspension concentrate

According to above-mentioned nine examples, carry out the field control wheat powdery mildew test of 30% pyraclostrobin · picoxystrobin suspending agent and other compound formulations, pass simultaneously with 15% pyraclostrobin suspending concentrate, 250g/ Comparison of the effect of L picoxystrobin suspension concentrate to verify the synergistic effect after compounding. The test site is Huaiyang County, Zhoukou City, Henan Province. The time was March 20 and March 27, 2014, and the pesticide was applied twice. There were four treatments in the test, and each treatment was replicated three times. Each replicate had 6 clusters. Arranged randomly, the area of the plot is 15m ² , the planting habits of all plots are the same as the local planting habits, and the weather data on the day of spraying and the following week are recorded, as follows:

Table 2 Meteorological data during the test period

The survey standard is: survey once before spraying, and then survey once every 7 days after spraying, and randomly survey 40 wheat plants for each repetition (check the upper 3 leaves of 3 adjacent tillers). It is recorded according to the relevant standards in the "Pesticide Field Efficacy Test Standards". The experimental data adopts Duncan's new multiple range detection method (DMRT).

Table 3 Control effect of different treatments on wheat powdery mildew

From table 3 test result shows, after spraying, the control effect of medicament to wheat powdery mildew is obviously higher than that of comparison medicament, and after 7 days of secondary spraying, the control effect of medicament to powdery mildew still remains between 70-85%. Wherein the control effect of example five is the highest 82.91%. This shows that pyraclostrobin and picoxystrobin compound fungicides have a better synergistic effect, and the duration of effect is significantly longer than that of a single agent. Experiments show that the compound composition obviously reduces the dosage of chemicals used, reduces production costs, pesticide residues and environmental pollution. In addition, according to field observations during the test, each treatment solution of the medicaments in the examples has no effect on crop growth, is beneficial to the surrounding biological safety, and has good compatibility with the environment. It can be seen that pyraclostrobin and picoxystrobin are an ideal compound sterilization product.

Claims (9)

1. a bactericidal composition, is characterized in that, active ingredient is pyraclostrobin and ZEN 90160, and formulation is suspending agent, water dispersible granules.

2. bactericidal composition according to claim 1, is characterized in that, the weight ratio of described active ingredient pyraclostrobin and ZEN 90160 is 1:20 ~ 15:1.

3. bactericidal composition according to claim 2, is characterized in that, the weight ratio of described active ingredient pyraclostrobin and ZEN 90160 is 1:8 ~ 5:1.

4. bactericidal composition according to claim 3, is characterized in that, described active ingredient pyraclostrobin and the weight percentage of ZEN 90160 in described bactericidal composition are 10% ~ 80%.

5. bactericidal composition according to claim 4, is characterized in that, also comprises carrier and auxiliary agent in described bactericidal composition.

6. bactericidal composition according to claim 5, is characterized in that, described carrier be selected from water, filler one or more.

7. bactericidal composition according to claim 5, is characterized in that, described auxiliary agent comprises surfactant, described surfactant be selected from emulsifier, dispersant, wetting agent or bleeding agent one or more.

8. bactericidal composition according to claim 7, is characterized in that, described auxiliary agent also comprises other functional aids, and other functional aids described to be selected from antifreezing agent, thickener, stabilizing agent, disintegrant or defoamer one or more.

9. the application of bactericidal composition according to any one of a claim 1 ~ 8 on control wheat powdery mildew.