CN105311052A - 一种小鼠色素缺失动物模型的制备方法 - Google Patents
一种小鼠色素缺失动物模型的制备方法 Download PDFInfo
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- CN105311052A CN105311052A CN201510689561.1A CN201510689561A CN105311052A CN 105311052 A CN105311052 A CN 105311052A CN 201510689561 A CN201510689561 A CN 201510689561A CN 105311052 A CN105311052 A CN 105311052A
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- 238000010171 animal model Methods 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 239000000049 pigment Substances 0.000 claims abstract description 60
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims abstract description 12
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 6
- 241000699670 Mus sp. Species 0.000 claims description 41
- 241000699666 Mus <mouse, genus> Species 0.000 claims description 14
- 206010010356 Congenital anomaly Diseases 0.000 claims description 12
- 238000009395 breeding Methods 0.000 claims description 6
- 230000001488 breeding effect Effects 0.000 claims description 6
- 230000027326 copulation Effects 0.000 claims description 6
- 238000007920 subcutaneous administration Methods 0.000 claims description 3
- 210000003371 toe Anatomy 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 4
- 239000003814 drug Substances 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 238000011156 evaluation Methods 0.000 abstract description 3
- 230000008506 pathogenesis Effects 0.000 abstract description 3
- 238000012217 deletion Methods 0.000 abstract 3
- 230000037430 deletion Effects 0.000 abstract 3
- 229940079593 drug Drugs 0.000 abstract 1
- 201000010099 disease Diseases 0.000 description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 9
- 230000008034 disappearance Effects 0.000 description 4
- 206010025421 Macule Diseases 0.000 description 2
- 238000010172 mouse model Methods 0.000 description 2
- 241000345998 Calamus manan Species 0.000 description 1
- 208000003367 Hypopigmentation Diseases 0.000 description 1
- 208000026748 Hypopigmentation disease Diseases 0.000 description 1
- 201000011252 Phenylketonuria Diseases 0.000 description 1
- 206010047642 Vitiligo Diseases 0.000 description 1
- 230000035614 depigmentation Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000004392 genitalia Anatomy 0.000 description 1
- 230000003425 hypopigmentation Effects 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 230000037311 normal skin Effects 0.000 description 1
- 201000007909 oculocutaneous albinism Diseases 0.000 description 1
- 201000009442 piebaldism Diseases 0.000 description 1
- 208000037920 primary disease Diseases 0.000 description 1
- 235000012950 rattan cane Nutrition 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 208000009999 tuberous sclerosis Diseases 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/04—Sulfur, selenium or tellurium; Compounds thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
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- Pharmacology & Pharmacy (AREA)
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- Animal Behavior & Ethology (AREA)
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Abstract
本发明公开了一种小鼠色素缺失动物模型的制备方法，其通过将稀硫酸或稀盐酸注射于新生小鼠不同部位皮下，连续7日，继续喂养至6周龄以上，可培养获得后天部分皮肤色素缺失小鼠。该方法操作简便，获得的动物模型性状可遗传，为色素缺失症的发病机理研究、开发治疗色素缺失症的药物或方法、评价各种治疗色素缺失症的疗效提供了适宜的动物模型。
Description
技术领域
本发明涉及一种小鼠色素缺失动物模型的制备方法。
背景技术
色素缺失症是常见的一种皮肤病症，是指皮肤、粘膜处出现比正常肤色浅的斑片，主要是由于皮肤色素减退或色素脱失素所造成的。白斑可以是先天性的，也可以是后天性的。色素缺失症白斑可发生于全身任何部位，但以面部、颈部、手背等暴露部位及外生殖器等皱褶处皮肤多见,多数为局限性，还可以全身性。本病治疗困难，疗程较长，痊愈机会较少。非全身性皮肤色素缺失包括白癜风、结节性硬化病、斑驳病、伊藤色素减退症等。全身性皮肤色素缺失包括眼皮肤白化病综合症、苯丙酮尿症。色素缺失症可发生于任何年龄段，尤其是青少年，色素缺失严重影响其心理,导致自卑、内向，甚至暴力倾向。
发明内容
为了对人类白化病等进行进一步研究并获得有效治疗方法，本发明提供一种小鼠色素缺失动物模型的制备方法。
为实现上述目的，本发明采取的技术方案是：
一种小鼠色素缺失动物模型的制备方法，将10-50%的稀硫酸或10-20%的稀盐酸0.1-0.5ml注射于新生当日小鼠不同部位皮下，1次/日，连续7日，继续喂养至6周龄以上，获得后天部分皮肤色素缺失小鼠，该性状可遗传。
将后天部分皮肤色素缺失的雌鼠、雄鼠进行交配，繁殖获得第一代先天部分皮肤色素缺失小鼠；将第一代先天部分皮肤色素缺失的雌鼠、雄鼠进行交配，繁殖获得第二代先天部分皮肤色素缺失小鼠；以此类推，稳定获得先天部分皮肤色素缺失小鼠。
本发明优点在于：
1、本发明建立的小鼠色素缺失动物模型具有色素缺失部位选择性缺失的特点，克服以往只有全身性色素缺失的白化病小鼠模型；
2、建立的小鼠色素缺失动物模型为后天小鼠色素缺失动物模型，该性状可遗传；
3、本发明的后天小鼠色素缺失动物模型为色素缺失症的发病机理研究、开发治疗色素缺失症的药物或方法、评价各种治疗色素缺失症的疗效提供了适宜的动物模型。
具体实施方式
实施例1
一种小鼠色素缺失动物模型的制备方法，将10-50%的稀硫酸或10-20%的稀盐酸将后天部分皮肤色素缺失的雌鼠、雄鼠进行交配，繁殖获得第一代先天部分皮肤色素缺失小鼠；将第一代先天部分皮肤色素缺失的雌鼠、雄鼠进行交配，繁殖获得第二代先天部分皮肤色素缺失小鼠；以此类推，稳定获得先天部分皮肤色素缺失小鼠。
本发明优点在于：
1、本发明建立的小鼠色素缺失动物模型具有色素缺失部位选择性缺失的特点，克服以往只有全身性色素缺失的白化病小鼠模型；
2、建立的小鼠色素缺失动物模型为后天小鼠色素缺失动物模型，该性状可遗传；
3、本发明的后天小鼠色素缺失动物模型为色素缺失症的发病机理研究、开发治疗色素缺失症的药物或方法、评价各种治疗色素缺失症的疗效提供了适宜的动物模型。
具体实施方式
实施例1
一种小鼠色素缺失动物模型的制备方法，将10-50%的稀硫酸或10-20%的稀盐酸日，连续7日，继续喂养至6周龄以上，获得后天足趾色素缺失小鼠。
实施例3
一种小鼠色素缺失动物模型的制备方法，将10-50%的稀硫酸或10-20%的稀盐酸0.3ml注射于新生当日小鼠背部皮下，1次/日，连续7日，继续喂养至6周龄以上，获得后天背部色素缺失小鼠。
Claims (6)
1.一种小鼠色素缺失动物模型的制备方法，其特征是将10-50%的稀硫酸或10-20%的稀盐酸0.1-0.5ml注射于新生当日小鼠不同部位皮下，1次/日，连续7日，继续喂养至6周龄以上，获得后天部分皮肤色素缺失小鼠。
2.按照权利要求1所述的一种小鼠色素缺失动物模型的制备方法，其特征在于：所述小鼠不同部位是鼠尾。
3.按照权利要求1所述一种小鼠色素缺失动物模型的制备方法，其特征在于：所述小鼠不同部位是足趾。
4.按照权利要求1所述一种小鼠色素缺失动物模型的制备方法，其特征在于：所述小鼠不同部位是背部。
5.按照权利要求1所述一种小鼠色素缺失动物模型的制备方法，其特征在于：所述小鼠为实验用小鼠。
6.按照权利要求1所述一种小鼠色素缺失动物模型的制备方法，其特征在于：将后天部分皮肤色素缺失的雌鼠、雄鼠进行交配，繁殖获得第一代先天部分皮肤色素缺失小鼠；将第一代先天部分皮肤色素缺失的雌鼠、雄鼠进行交配，繁殖获得第二代先天部分皮肤色素缺失小鼠；以此类推，稳定获得先天部分皮肤色素缺失小鼠。
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090232758A1 (en) * | 2008-03-13 | 2009-09-17 | L V M H Recherche | Brassocattleya marcella koss orchid extract and use thereof as skin depigmentation agent |
| WO2013189930A1 (en) * | 2012-06-19 | 2013-12-27 | L'oreal | Process for depigmenting keratin materials using novel resorcinol-based compounds |
| CN103919798A (zh) * | 2014-03-26 | 2014-07-16 | 许爱娥 | 一种白癜风动物模型的构建方法 |
| CN104263754A (zh) * | 2014-08-29 | 2015-01-07 | 中国科学院广州生物医药与健康研究院 | 白化病模型猪的重构卵及其构建方法和模型猪的构建方法 |
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2015
- 2015-10-23 CN CN201510689561.1A patent/CN105311052A/zh active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090232758A1 (en) * | 2008-03-13 | 2009-09-17 | L V M H Recherche | Brassocattleya marcella koss orchid extract and use thereof as skin depigmentation agent |
| WO2013189930A1 (en) * | 2012-06-19 | 2013-12-27 | L'oreal | Process for depigmenting keratin materials using novel resorcinol-based compounds |
| CN103919798A (zh) * | 2014-03-26 | 2014-07-16 | 许爱娥 | 一种白癜风动物模型的构建方法 |
| CN104263754A (zh) * | 2014-08-29 | 2015-01-07 | 中国科学院广州生物医药与健康研究院 | 白化病模型猪的重构卵及其构建方法和模型猪的构建方法 |
Non-Patent Citations (3)
| Title |
|---|
| 薛东章，等: "皮肤脱色剂研究方法", 《中华全科医学》 * |
| 雷铁池，等: "皮肤脱色剂的分子机制及其相关研究", 《国外医学皮肤性病学分册》 * |
| 龙子江，等: "化学脱色法制备白癜风动物模型", 《安徽中医学院学报》 * |
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