CN105181972A - Constant temperature antibody incubation box - Google Patents
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- CN105181972A CN105181972A CN201510571561.1A CN201510571561A CN105181972A CN 105181972 A CN105181972 A CN 105181972A CN 201510571561 A CN201510571561 A CN 201510571561A CN 105181972 A CN105181972 A CN 105181972A
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- 238000011534 incubation Methods 0.000 title claims abstract description 43
- 238000005192 partition Methods 0.000 claims abstract description 16
- 238000004321 preservation Methods 0.000 claims abstract description 15
- 238000007789 sealing Methods 0.000 claims abstract description 15
- 238000009413 insulation Methods 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 8
- 238000001704 evaporation Methods 0.000 claims abstract description 5
- 230000008020 evaporation Effects 0.000 claims abstract description 5
- 230000000694 effects Effects 0.000 claims description 5
- 239000012528 membrane Substances 0.000 abstract description 17
- 238000002474 experimental method Methods 0.000 abstract description 11
- 238000013461 design Methods 0.000 abstract description 7
- 239000005457 ice water Substances 0.000 abstract description 5
- 239000000463 material Substances 0.000 abstract description 4
- 239000000203 mixture Substances 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 230000015556 catabolic process Effects 0.000 abstract description 3
- 238000006731 degradation reaction Methods 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000001262 western blot Methods 0.000 description 2
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000003748 differential diagnosis Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000002055 immunohistochemical effect Effects 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 238000010827 pathological analysis Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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Abstract
本发明公开一种恒温抗体孵育盒，包括保温盒体1、盒盖2和密封圈3。保温盒体1内设有若干隔板4、内腔5和内腔盖6。保温盒体1与盒盖2利用密封圈3实现密封、防液体蒸发及漏液的作用。隔板4的高度小于保温盒体1高度，但大于或等于1/2保温盒体1高度。隔板4可与内腔5的外腔壁直接相连或不相连。本发明的有益效果是：保温盒体内设有可紧密扣合的内腔和内腔盖，可通过放置冰水混合物或蓄冷能力较强材料，保证恒温孵育，避免抗体降解，提高实验重现性；圆柱形保温盒体设计有效避免膜或切片贴附在保温盒体上，避免膜或切片孵育不完全；可通过调节隔板的数量及与内腔外壁相连或不相连的方式调节孵育空间，提高一次性孵育膜或切片的数量。
The invention discloses a constant temperature antibody incubation box, which comprises a heat preservation box body 1 , a box cover 2 and a sealing ring 3 . The insulation box body 1 is provided with a plurality of partitions 4 , an inner cavity 5 and an inner cavity cover 6 . The heat preservation box body 1 and the box cover 2 utilize the sealing ring 3 to realize sealing and prevent liquid evaporation and liquid leakage. The height of the partition plate 4 is less than the height of the insulation box body 1, but greater than or equal to 1/2 the height of the insulation box body 1. The partition 4 may be directly connected to the outer cavity wall of the inner cavity 5 or not. The beneficial effects of the present invention are: the inner cavity and the inner cavity cover that can be tightly fastened are provided in the heat preservation box, and the ice-water mixture or the material with strong cold storage capacity can be placed to ensure constant temperature incubation, avoid antibody degradation, and improve experiment reproducibility ;The design of the cylindrical incubator effectively prevents the film or slice from being attached to the incubator and prevents the incomplete incubation of the film or slice; the incubation space can be adjusted by adjusting the number of partitions and connecting or not connecting with the inner and outer walls. Increase the number of membranes or sections incubated at once.
Description
技术领域technical field
本发明涉及生物技术以及医疗卫生领域的孵育装置，具体是指一种恒温抗体孵育装置。The invention relates to an incubation device in the fields of biotechnology and medical care, in particular to a constant temperature antibody incubation device.
背景技术Background technique
蛋白质免疫印迹技术和免疫组织化学是分子生物学实验以及临床肿瘤病理诊断中最常用的技术。在蛋白质免疫印迹实验中，当蛋白通过电泳和转膜技术被转移并固定在硝酸纤维素或尼龙膜上后，需将膜与特定的抗体溶液进行共孵育，再经漂洗、显色等步骤即可观察抗体与蛋白中特异抗原成份的结合，并进一步用于检测样品中特定蛋白的含量变化。免疫组织化学实验中也需将组织切片与抗体溶液进行共孵育，再经漂洗、显色等步骤即可对临床病理组织进行肿瘤诊断或鉴别诊断。因此膜或切片与抗体溶液共孵育是实验的关键步骤。目前抗体孵育的器皿设计比较简单，如杂交袋在使用过程中容易产生气泡，导致抗体与膜不能完全浸润。孵育保温盒体积较大，易浪费昂贵的抗体试剂，且膜或切片容易贴于盒底，造成孵育不完全，直接影响结果的重现性。另一方面，为了保证实验的重现性、避免抗体的降解，抗体孵育过程需在恒温或低温下进行。但目前使用的孵育盒没有蓄冷能力，孵育效果不理想。如将抗体孵育盒放入碎冰摇床中，融化的冰水容易渗入孵育液，会影响实验结果。Western blotting and immunohistochemistry are the most commonly used techniques in molecular biology experiments and clinical tumor pathological diagnosis. In western blotting experiments, after proteins are transferred and fixed on nitrocellulose or nylon membranes by electrophoresis and membrane transfer techniques, the membranes need to be co-incubated with a specific antibody solution, and then rinsed and developed. The binding of antibodies to specific antigenic components in proteins can be observed, and further used to detect changes in the content of specific proteins in samples. In immunohistochemical experiments, it is also necessary to co-incubate tissue sections with antibody solutions, and then perform steps such as rinsing and color development to perform tumor diagnosis or differential diagnosis on clinical pathological tissues. Therefore, co-incubation of membrane or slice with antibody solution is a key step in the experiment. At present, the design of the antibody incubation vessel is relatively simple. For example, the hybridization bag is prone to air bubbles during use, resulting in incomplete infiltration of the antibody and the membrane. The incubation box is large in size, which is easy to waste expensive antibody reagents, and the membrane or slices are easy to stick to the bottom of the box, resulting in incomplete incubation, which directly affects the reproducibility of the results. On the other hand, in order to ensure the reproducibility of the experiment and avoid the degradation of the antibody, the antibody incubation process needs to be carried out at constant temperature or low temperature. However, the currently used incubation boxes have no cold storage capacity, and the incubation effect is not ideal. If the antibody incubation box is placed in a crushed ice shaker, the melted ice water will easily seep into the incubation solution, which will affect the experimental results.
发明内容Contents of the invention
为了解决现有技术所存在的上述不足，本发明提出一种结构简单，设计巧妙，能够大批量的进行抗体孵育，且兼有保温能力的抗体孵育盒。In order to solve the above-mentioned deficiencies in the prior art, the present invention proposes an antibody incubation box with simple structure, ingenious design, capable of mass-incubating antibodies, and having the ability to keep warm.
一种恒温抗体孵育盒，包括保温盒体1、盒盖2和密封圈3，在保温盒体1内设有若干隔板4、内腔5和内腔盖6。所述保温盒体1与盒盖2利用密封圈3实现密封、防液体蒸发及漏液的作用。所述隔板4的高度小于保温盒体1高度，但大于或等于1/2保温盒体1高度。所述隔板4可与内腔5的外腔壁直接相连或不相连。所述内腔5和内腔盖6可紧密扣合，有效实现保温效果。A constant temperature antibody incubation box includes a heat preservation box body 1, a box cover 2 and a sealing ring 3, and a plurality of partitions 4, an inner chamber 5 and an inner chamber cover 6 are arranged in the heat preservation box body 1. The heat preservation box body 1 and the box cover 2 utilize the sealing ring 3 to realize sealing and prevent liquid evaporation and liquid leakage. The height of the partition 4 is less than the height of the insulation box body 1, but greater than or equal to 1/2 of the height of the insulation box body 1. The partition 4 may be directly connected to the outer cavity wall of the inner cavity 5 or not. The inner cavity 5 and the inner cavity cover 6 can be tightly fastened to effectively realize the heat preservation effect.
该孵育盒配有盒盖和密封圈，具有很好的密封效果，可有效避免孵育过程中液体的蒸发损失及漏液。在内腔内放置冰水混合物或蓄冷能力较强材料，并盖以内盖密封，可长时间保持低温或一定温度，保障了实验的重现性和抗体的稳定性。此外，可根据放入的膜或切片的数量，选择在孵育盒内采用与内腔外壁相连或不相连的方式使用一个或多个隔板，以方便控制孵育空间，提高孵育效率。此外，孵育盒圆柱形的设计可在保证膜或切片与抗体孵育液均匀作用的同时，防止膜或切片贴附于孵育盒上，有利于提高同时孵育膜或切片的数量，节省抗体用量。The incubation box is equipped with a lid and a sealing ring, which has a good sealing effect and can effectively avoid liquid evaporation loss and leakage during incubation. The ice-water mixture or materials with strong cold storage capacity are placed in the inner cavity, and the inner cover is sealed to keep low temperature or a certain temperature for a long time, which ensures the reproducibility of the experiment and the stability of the antibody. In addition, according to the number of membranes or slices put in, one or more partitions can be selected to be connected or not connected to the inner and outer walls of the incubation box, so as to facilitate the control of the incubation space and improve the incubation efficiency. In addition, the cylindrical design of the incubation box can ensure the uniform interaction between the membrane or slice and the antibody incubation solution, and at the same time prevent the membrane or slice from adhering to the incubation box, which is beneficial to increase the number of membranes or slices incubated at the same time and save the amount of antibody.
本发明同现有技术相比，具有如下优点：Compared with the prior art, the present invention has the following advantages:
本发明适用于抗体恒温孵育，其结构简单，设计巧妙，布局合理。它可以使抗体的孵育温度保持恒定低温，避免抗体降解且可节省抗体孵育溶液用量，使抗体孵育操作变得重现性强、可控性好，还可一次性完成多个膜或切片的平行实验操作，减轻工作人员的劳动负担，提高工作效率。并且其圆柱形的孵育保温盒体设计可以防止膜/切片贴附在孵育保温盒体上，避免膜/切片孵育不完全，消除了实验的假阳性，提高了实验重现性。它的制作工艺简单，制造成本低廉，因此可以说它具备了多种优点，特别适合于在本领域中推广应用，其市场前景十分广阔。The invention is suitable for constant temperature incubation of antibodies, and has simple structure, ingenious design and reasonable layout. It can keep the incubation temperature of the antibody at a constant low temperature, avoid antibody degradation and save the amount of antibody incubation solution, make the antibody incubation operation reproducible and controllable, and can complete the parallelization of multiple membranes or slices at one time Experimental operation reduces the labor burden of the staff and improves work efficiency. And its cylindrical incubation box design can prevent the membrane/section from adhering to the incubation box, avoid incomplete incubation of the membrane/section, eliminate false positives in the experiment, and improve the reproducibility of the experiment. Its manufacturing process is simple and its manufacturing cost is low, so it can be said that it has many advantages, and it is especially suitable for popularization and application in this field, and its market prospect is very broad.
说明书附图Instructions attached
图1为本发明保温盒体的主视图。Fig. 1 is a front view of the heat preservation box of the present invention.
图2为本发明保温盒体的俯视图。Fig. 2 is a top view of the heat preservation box of the present invention.
图3为本发明盒盖的主视图。Fig. 3 is the front view of the box cover of the present invention.
图4为本发明盒盖的仰视图。Fig. 4 is a bottom view of the box cover of the present invention.
图5为本发明保温盒体的立体俯视图。Fig. 5 is a three-dimensional top view of the heat preservation box of the present invention.
图6为本发明盒盖的立体图。Fig. 6 is a perspective view of the box cover of the present invention.
图7为本发明内腔盖的立体图。Fig. 7 is a perspective view of the inner cavity cover of the present invention.
图8为本发明密封圈的立体图。Fig. 8 is a perspective view of the sealing ring of the present invention.
具体实施方式Detailed ways
下面将结合附图说明本发明的具体实施方式。如图1-8所示：一种恒温抗体孵育盒，包括保温盒体1、盒盖2和密封圈3。保温盒体1内设有若干隔板4、内腔5和内腔盖6。保温盒体1与盒盖2利用密封圈3实现密封、防液体蒸发及漏液的作用。隔板4的高度小于保温盒体1高度，但大于或等于1/2保温盒体1高度。隔板4可与内腔5的外腔壁直接相连或不相连。有利于同时进行孵育相同抗体、不同膜/切片的平行实验，减少孵育抗体的体积，节省抗体用量。在内腔5内可通过放置冰水混合物或蓄冷能力较强材料，并通过与内腔盖6的紧密扣合实现长时间保持低温或一定温度的目的，以保障实验的重现性和抗体的稳定性。保温盒体1圆柱形的设计还可有效防止膜或切片贴附在装置上造成的孵育不完全。The specific implementation manner of the present invention will be described below with reference to the accompanying drawings. As shown in Figure 1-8: a constant temperature antibody incubation box, including a heat preservation box body 1, a box cover 2 and a sealing ring 3. A plurality of partitions 4 , an inner chamber 5 and an inner chamber cover 6 are arranged in the heat preservation box body 1 . The heat preservation box body 1 and the box cover 2 utilize the sealing ring 3 to realize sealing and prevent liquid evaporation and liquid leakage. The height of the dividing plate 4 is less than the height of the insulation box body 1, but greater than or equal to 1/2 the height of the insulation box body 1. The partition 4 may be directly connected to the outer cavity wall of the inner cavity 5 or not. It is beneficial to carry out parallel experiments incubating the same antibody and different membranes/sections at the same time, reducing the volume of incubating antibodies and saving the amount of antibodies used. In the inner cavity 5, the ice-water mixture or materials with strong cold storage capacity can be placed, and the purpose of keeping a low temperature or a certain temperature for a long time can be achieved by tightly fastening with the inner cavity cover 6, so as to ensure the reproducibility of the experiment and the stability of the antibody. stability. The cylindrical design of the insulation box body 1 can also effectively prevent incomplete incubation caused by the attachment of the membrane or slices to the device.
本发明实施例的恒温抗体孵育盒的工作过程如下：需要对膜或切片进行抗体孵育处理时，首先在内腔内放置冰水混合物或蓄冷能力较强材料，并紧密扣合内腔盖。然后将膜或切片放置于保温盒体的隔板之间，根据放入的膜或切片的数量选择一个或多个隔板，隔板与保温盒体内壁、内腔外壁形成的空间即为抗体孵育空间，在此空间内加入抗体溶液进行抗体孵育。The working process of the constant temperature antibody incubation box of the embodiment of the present invention is as follows: when it is necessary to incubate the membrane or slice with antibodies, first place the ice-water mixture or a material with strong cold storage capacity in the inner cavity, and tightly fasten the inner cavity cover. Then place the film or slices between the partitions of the insulation box, and select one or more partitions according to the number of films or slices put in. The space formed by the partitions, the inner wall of the insulation box, and the outer wall of the inner cavity is the antibody Incubation space, add antibody solution in this space for antibody incubation.
以上所述，仅为本发明较佳的具体实施方式，但本发明的保护范围并不局限于此，任何熟悉本技术领域的技术人员在本发明披露的技术范围内，根据本发明的技术方案及其发明构思加以等同替换或改变，都应涵盖在本发明的保护范围之内。The above is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto, and any person familiar with the technical field within the technical scope disclosed in the present invention, according to the technical solution of the present invention Any equivalent replacement or change of the inventive concepts thereof shall fall within the protection scope of the present invention.
Claims (5)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510571561.1A CN105181972A (en) | 2015-09-09 | 2015-09-09 | Constant temperature antibody incubation box |
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| CN201510571561.1A CN105181972A (en) | 2015-09-09 | 2015-09-09 | Constant temperature antibody incubation box |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108318678A (en) * | 2018-02-01 | 2018-07-24 | 江苏省肿瘤防治研究所（江苏省肿瘤医院） | A kind of rotary antibody incubation device of water wheels |
| CN112903992A (en) * | 2021-02-04 | 2021-06-04 | 四川大学华西医院 | Efficient immunoblotting antibody incubation device and incubation method |
-
2015
- 2015-09-09 CN CN201510571561.1A patent/CN105181972A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108318678A (en) * | 2018-02-01 | 2018-07-24 | 江苏省肿瘤防治研究所（江苏省肿瘤医院） | A kind of rotary antibody incubation device of water wheels |
| CN112903992A (en) * | 2021-02-04 | 2021-06-04 | 四川大学华西医院 | Efficient immunoblotting antibody incubation device and incubation method |
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Application publication date: 20151223 |