CN105078803B - Use of Callicarpa plant extract - Google Patents
Use of Callicarpa plant extract Download PDFInfo
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- CN105078803B CN105078803B CN201410208522.0A CN201410208522A CN105078803B CN 105078803 B CN105078803 B CN 105078803B CN 201410208522 A CN201410208522 A CN 201410208522A CN 105078803 B CN105078803 B CN 105078803B
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- callicarpa
- extract
- beautyberry
- water
- plant
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Images
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- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides application of a callicarpa plant extract in preparing cosmetics for removing freckles, whitening, removing wrinkles, preventing sunburn or resisting ageing. The Callicarpa plant extract has effects of inhibiting tyrosinase activity, scavenging free radicals, reducing ultraviolet absorption, and resisting skin aging. The beautyberry plant has wide source, long use history and simple preparation process of the extract. The beautyberry plant extract is an economic, applicable, safe and effective cosmetic functional raw material. The invention also provides a cosmetic of the beautyberry plant extract.
Description
Technical Field
The invention relates to the field of cosmetics, in particular to application of a beautyberry plant extract in preparation of cosmetics.
Background
The skin is the largest organ tissue of the human body and can protect the body from external harmful factors (such as ultraviolet rays, chemical irritants, heavy metals and some exogenous pollutants). In addition to natural function degradation caused by age, factors such as modern fast-paced, high-stress lifestyle, unregulated diet, environmental deterioration accelerate the degradation and aging of skin functions, resulting in a series of skin problems.
Modern researches suggest that the human body can generate excessive active oxygen free radicals in the metabolic process, and if the active oxygen free radicals are not removed in time, the active oxygen free radicals can generate oxidative damage to the skin, so that the skin is aged, denatured, relaxed, and elastic, and wrinkles are formed. Therefore, scavenging reactive oxygen species is one of the important means to prevent and ameliorate the aging problem of skin. Therefore, people use various anti-aging cosmetics, which mainly play a role in resisting oxidation by adding antioxidants (such as SOD, vitamin E and the like), and the cosmetics have certain anti-aging effect, but most of the cosmetics cannot effectively solve the skin problems.
The Callicarpa is a general name of plants in Callicarpa (Callicarpa L.) of Verbenaceae (Verbenaceae), the varieties are various, about 190, according to records of Chinese plant record, about 46 plants are recorded in China, and the distribution is wide. The folium Callicarpae Formosanae contains main components such as flavonoids, terpenes, phenylethanoid glycosides and volatile oils. Originally written in "materia medica shiyi" by the ancient depository of Tang Kaiyuan, it is recorded in many places of materia medica books of the past generations, and has the functions of sterilization, antiphlogosis, hemostasis, etc., as recorded in "materia medica shiyi": to relieve all toxicants, carbuncle-abscess, pharyngitis, insect poison, toxin swelling, fistula, poisonous snake and poisonous mania, and to take after boiling, and also to wash away sore and swelling, remove blood and grow muscle, recorded in compendium of materia Medica: "activating blood and subduing swelling, inducing diuresis to remove toxicity, treating all carbuncle and deep-rooted carbuncle and various swollen toxins with back bleeding", recorded in Fujian folk herbal medicine: promoting blood stasis, stopping bleeding, diminishing inflammation and resolving depression. In recent years, various efficacies of beautyberry have been reported, for example, chinese patent application No.200810189027.4 discloses a beautyberry toothpaste with heat-clearing, anti-inflammatory, hemostatic, bacteriostatic and analgesic effects. Chinese patent application No.200910002488.0 discloses folium Callicarpae Formosanae collutory with effects of clearing heat, relieving inflammation, stopping bleeding, inhibiting bacteria, and relieving pain. Chinese patent application No.201110210953.7 discloses the use of Callicarpa nudiflora extract in daily chemical cleaning products. Chinese patent application No.200910036486.3 discloses the use of beautyberry extract in oral cleaning products to alleviate various symptoms of oral gingival bleeding, gingival inflammation and oral ulcer that people often suffer from. At present, the application of callicarpa in cosmetics with functions of removing freckles, whitening, removing wrinkles, and preventing sun or aging is not reported.
Disclosure of Invention
The invention aims to provide application of a beautyberry plant extract in preparing cosmetics. The cosmetic has effects of resolving macula, whitening skin, removing wrinkle, preventing sunburn or resisting aging.
The invention provides the following technical scheme:
1. use of Callicarpa plant extract in preparing cosmetic for removing speckle, whitening skin, removing wrinkle, and preventing sunburn or aging is provided.
2. The use according to claim 1, wherein the cosmetic comprises 0.1-10.0% by weight of beautyberry extract, preferably 0.5-5.0% by weight of beautyberry extract, more preferably 1.0-3.0% by weight of beautyberry extract.
3. The use according to claim 1 or 2, wherein the beautyberry plant extract is prepared from the stem and/or leaf of beautyberry plant of the family Verbenaceae.
4. The use according to any one of technical schemes 1-3, wherein the Callicarpa plant of the family Verbenaceae is selected from Callicarpa acutifolia (Callicarpa acutifolia H.T. Chang), Callicarpa heterophylla (Callicarpa anisophila), Callicarpa arborescens (Callicarpa arborescens Roxb. Hort. Beng), Callicarpa bodinieri (Callicarpa arborescens Levl.), Callicarpa brachycarpa (Benth. hand), Callicarpa alba (Callicarpa Candidyma. F.) Hochr, Callicarpa californica (Callicarpa cathayana H.T. Chang), Callicarpa cloudbury (Callicarpa purpurea) P' ei et W.Z.Fang), Callicarpa californica (Callicarpa cathayensis H.T. Chang), Callicarpa calixarisonia californica purpurea (Callicarpa, Callicarpa grandiflora H.T. multidentate), Callicarpa arborescens (Callicarpa, Callicarpa red Callicarpa japonica (Callicarpa japonica Thunb), Callicarpa japonica (Callicarpa kochiana Makino), Callicarpa rubra (Callicarpa kotoensis Hayata), Callicarpa guangdongensis (Callicarpa kwangtungensis Chun), Callicarpa glabrata (Callicarpa lingeri Merr), Callicarpa acuminata (Callicarpa lobayensis C.), Callicarpa longata (Callicarpa longstretch, Callicarpa longata (Callicarpa longwall. Merr), Callicarpa longata (Callicarpa longifornia Lamk., Callicarpa long, Callicarpa longata (Callicarpa longwall. dunn.), Callicarpa (Callicarpa grandiflora), Callicarpa glabra (Callicarpa longwall. straight.), Callicarpa red (Callicarpa grandiflora. C.), Callicarpa grandiflora H. Changescholara, Callicarpa indica. Wwang, Callicarpa (Callicarpa grandiflora. straight) Pseudo-red beauty-berry (Callicarpa pseudo-oruberella H.T.Chang), majora beauty-berry (Callicarpa randaiensis Hayata), Shuchikutaea (Callicarpa remotisserrulata Hayata), Red beauty-berry (Callicarpa rubella Lindl.), Callicarpa sapida P' ei W.Z.Fang), Liriopsis margarita (Callicarpa satingwuensis H.T.Chang), and Yunan beauty-berry (Callicarpa yuensis W.Z.Fang).
5. The use according to any of claims 1-4, wherein the plant of the genus Callicarpa of the family Verbenaceae is selected from Callicarpa chinensis (Callicarpa cathayana H.T. Chang), Callicarpa Formosana Rolfe, Callicarpa macrophylla Vahl, and Callicarpa kwangtungensis Chun.
6. The use according to any one of claims 1 to 5, wherein the Callicarpa plant extract is prepared by a method comprising the steps of:
(1) crushing stems and/or leaves (preferably dried stems and/or leaves) of beautyberry plants, and extracting for 1-3 times by using a solvent, wherein the solvent is water, ethanol or a mixture of water and ethanol;
(2) mixing the extractive solutions, and concentrating under reduced pressure to remove the solvent in step (1); adding water with the volume 0.5 to 2 times that of the mixture, standing overnight, and centrifuging or filtering to obtain supernatant;
(3) passing the supernatant through chromatographic column filled with resin filler, washing with water to remove impurities, eluting with higher concentration alcohol water solution, collecting eluate, concentrating under reduced pressure, and drying to obtain Callicarpa plant extract.
7. The use according to claim 6, wherein in step (1), the extraction method is selected from the group consisting of flash extraction, reflux extraction, ultrasonic extraction, microwave extraction and percolation extraction.
8. The use according to claim 6 or 7, wherein in step (3), the resin filler is selected from the group consisting of macroporous adsorption resin, polyamide resin and ion exchange resin.
9. The use according to any of claims 6 to 8, wherein in step (3), the higher concentration aqueous alcohol solution is 30 to 90 vol% aqueous alcohol solution.
10. The use according to any of claims 1-9, wherein the cosmetic is a cream, milk, liquid, powder or spray.
11. A cosmetic comprising 0.1 to 10.0% of an extract of beautyberry, preferably 0.5 to 5.0% of an extract of beautyberry, more preferably 1.0 to 3.0% of an extract of beautyberry, which is prepared by the method as defined in any one of claims 6 to 9.
12. The cosmetic of the technical scheme 11 is cream, milk, liquid, powder or spray.
Drawings
FIG. 1 shows hydroxyl radical scavenging of beautyberry leaf extract at different concentrations, □, ◇, Iris pallida leaf extract, △, Guangdong beautyberry stem and leaf extract, ○, Callicarpa macrophylla stem extract, and vitamin C.
FIG. 2 shows DPPH radical scavenging by different concentrations of beautyberry extract, wherein □ is beautyberry leaf extract, ◇ is Dunhua leaf extract, △ is Callicarpa kwangtungensis stem leaf extract, ○ is Callicarpa macrophylla stem extract, and ++ vitamin C.
FIG. 3 shows the scavenging of superoxide anion by different concentrations of beautyberry extract, wherein □ is Callicarpa chinensis leaf extract, ◇ is Callicarpa pallida leaf extract, △ is Callicarpa kwangtungensis stem and leaf extract, ○ is Callicarpa macrophylla stem extract, and ++ isvitamin C.
FIG. 4 shows the inhibition of tyrosinase activity by beautyberry extract at different concentrations, wherein □ is beautyberry leaf extract, ◇ is Dunhua leaf extract, △ is Callicarpa kwangtungensis stem and leaf extract, ○ is Callicarpa macrophylla stem extract and arbutin.
Detailed description of the invention
The inventor surprisingly finds that the beautyberry plant extract has the functions of removing freckles, whitening, sun-screening, removing wrinkles or resisting aging, and has wide prospects in the aspect of developing cosmetics by using beautyberry plants, particularly developing the cosmetics for removing freckles, whitening, sun-screening, removing wrinkles or resisting aging.
In one aspect, the present invention provides the use of an extract of a plant of the genus callicarpa for the preparation of a cosmetic. According to the invention, the beautyberry plant extract is particularly suitable for preparing cosmetics for removing freckles, whitening, removing wrinkles, preventing sunburn or resisting ageing.
According to the present invention, the beautyberry plant extract is prepared from the stem and/or leaf (preferably dried stem and/or leaf) of beautyberry plant of Verbenaceae by water extraction or alcohol extraction or water-alcohol mixing extraction method. Non-limiting examples of Callicarpa plants suitable for use in the present invention include but are not limited to Callicarpa acutifolia (Callicarpa acutifolia h.t. chang), Callicarpa heterophylla (Callicarpa anisophila), Callicarpa arborescens (Callicarpa arboreox.home.beng), Callicarpa bodinier (Callicarpa bodinier Levl.), Callicarpa brachycarpus (Callicarpa brevip (Benth.) Hance), Callicarpa albicans (Callicarpa cathayensis (burm.f.) Hochr.), Callicarpa cataria h.t. chang), Callicarpa cloudina Callicarpa (Callicarpa chinesis P. wench.z. fang), Callicarpa californica (Callicarpa crabapple), Callicarpa japonica (Callicarpa japonica h. t. chang), Callicarpa purpurea (Callicarpa indica. t. chant. chang), Callicarpa purpurea japonica (Callicarpa grandis. either r, Callicarpa arborescens (Callicarpa japonica, Callicarpa califoria japonica, Callicarpa californica indica (Callicarpa indica. h. h.h.r. h.h.h.r. chant Callicarpa japonica (Callicarpa kochiana Makino), Callicarpa rubra (Callicarpa kotoensis Hayata), Callicarpa kwangtungensis (Callicarpa kwangtungensis Chun), Callicarpa glabrata (Callicarpa lingensis Merr), Callicarpa acuminata (Callicarpa lingua Meter), Callicarpa longata (Callicarpa lingua H.T. Chang), Callicarpa longata (Callicarpa lingua Ml.lake), Callicarpa longata (Callicarpa longipes Dunn), Achillea carolina (Callicarpa lingua L.Merr.), Callicarpa flava (Callicarpa longwall), Callicarpa glabra (Callicarpa grandiflora), Callicarpa glabra grandiflora H.T., Callicarpa arborescens (Callicarpanoptera Hayata), Callicarpa rubra (Callicarpa rubella Lindl.), Callicarpa salifolia (Callicarpa salcifolia P' ei et W.Z.Fang), Callicarpa longata (Callicarpa division-saiensis Metc.), Callicarpa tripod (Callicarpa tingwuuensis H.T.Chang) and Callicarpa yunnanensis W.Z.Fang. In a preferred embodiment, the plant of the genus Callicarpa of the family Verbenaceae is selected from the group consisting of Callicarpa chinensis (Callicarpa cathayana H.T. Chang), Callicarpa Formosana Rolfe, Callicarpa macrophylla Vahl, and Callicarpa kwangtungensis Chun.
Specifically, the preparation method of the beautyberry plant extract comprises the following steps:
(1) crushing stems and/or leaves (preferably dried stems and/or leaves) of beautyberry plants, and extracting for 1-3 times by using a solvent, wherein the solvent is water, ethanol or a mixture of water and ethanol;
(2) mixing the extractive solutions, and concentrating under reduced pressure to remove the solvent in step (1); adding water with the volume 0.5 to 2 times that of the mixture, standing overnight, and centrifuging or filtering to obtain supernatant;
(3) passing the supernatant through chromatographic column filled with resin filler, washing with water to remove impurities, eluting with higher concentration alcohol water solution, collecting eluate, concentrating under reduced pressure, and drying to obtain Callicarpa plant extract.
In the above method, the extraction method in step (1) is selected from the group consisting of a flash extraction method, a reflux extraction method, an ultrasonic extraction method, a microwave extraction method and a percolation extraction method, wherein the flash extraction method, the reflux extraction method or the percolation extraction method is preferred, and the percolation extraction method is more preferred.
In the above method, the stem and/or leaf of the beautyberry plant in the step (1) is preferably dried. The stems and/or leaves of the beautyberry plant are generally crushed into coarse or coarsest powders as defined in the paradigms of the chinese pharmacopoeia 2010 edition.
In the above method, the mixture of ethanol and water in step (1) is preferably a 50 to 90 vol% ethanol aqueous solution, and more preferably a 70 to 90 vol% ethanol aqueous solution.
In the method, the amount of the solvent used in the step (1) is preferably 5-20 times (i.e., 5-20 ml of solvent per gram of medicinal material), and more preferably 10-12 times (i.e., 10-12 ml of solvent per gram of medicinal material).
In the above method, in the step (3), after concentrating the eluate under reduced pressure, the following operations are optionally performed one or more times: dissolving the concentrated solution with water, passing through chromatographic column filled with resin filler, washing with water solution to remove impurities, eluting with higher concentration alcohol water solution, collecting eluate, and concentrating under reduced pressure.
In the above process, the resin filler in step (3) is selected from, but not limited to, macroporous adsorption resins, polyamide resins and ion exchange resins. In one embodiment, the resin filler is preferably a macroporous adsorbent resin of HPD100, HPD200, D101, AB-8, SP825, ADS-7 type, more preferably a macroporous adsorbent resin of D101, AB-8 type.
In one embodiment, in the above method, the washing in step (3) is performed using an aqueous solution 2 to 8 times the volume of the resin column bed (preferably 6 times the volume of the resin column bed). The resin column bed volume varies depending on the amount of supernatant to be actually treated, for example, the ratio of the resin column bed volume to the supernatant volume to be treated may be about 1: 0.5-2.
In the above method, the higher concentration alcohol aqueous solution in the step (3) is 30 to 90 vol% alcohol aqueous solution, preferably 30 to 80 vol% alcohol aqueous solution, more preferably 30 to 70 vol% alcohol aqueous solution, and more preferably 30 to 50 vol% alcohol aqueous solution. The dosage of the catalyst is 2-5 times of the volume of the resin column bed layer, and preferably 3-4 times of the volume of the resin column bed layer. The resin column bed volume varies depending on the amount of supernatant to be actually treated, for example, the ratio of the resin column bed volume to the supernatant volume to be treated may be about 1: 0.5-2. In one embodiment, the alcohol is preferably ethanol.
The beautyberry plant extract can be combined with auxiliary material additives acceptable in cosmetics to prepare cosmetics for removing freckles, whitening, removing wrinkles, preventing sunburn or resisting ageing. The cosmetic may be prepared in any form that is cosmetically acceptable, such as, but not limited to, a cream, a milk, a liquid, a powder, or a spray, as desired. According to the present invention, the cosmetic contains 0.1 to 10.0% of the extract of beautyberry, preferably 0.5 to 5.0% of the extract of beautyberry, and more preferably 1.0 to 3.0% of the extract of beautyberry.
Cosmetically acceptable auxiliary additives suitable for use in the cosmetics of this invention include, but are not limited to, binders, lubricants, disintegrants, flavoring agents, antioxidants, emulsifiers, thickeners, preservatives, and the like.
Such binders include, but are not limited to, hydroxypropyl cellulose, corn starch, pregelatinized starch, modified corn starch, polyvinyl pyrrolidone, hydroxypropyl methylcellulose, lactose, acacia, ethylcellulose, cellulose acetate, and the like.
Such lubricants include, but are not limited to, magnesium stearate, zinc stearate, calcium stearate, talc, stearic acid, colloidal silicon dioxide, palmitic acid, and the like.
Such disintegrants include, but are not limited to, croscarmellose sodium, crospovidone, starch, potato starch, pregelatinized starch, corn starch, sodium starch glycolate, microcrystalline cellulose, hydroxypropyl cellulose, and the like.
The flavoring agents include, but are not limited to, fruit essences, plant essences, and the like.
Such antioxidants include, but are not limited to, Butylated Hydroxyanisole (BHA), Butylated Hydroxytoluene (BHT), Propyl Gallate (PG), ascorbic acid, α -tocopherol, and the like.
Such emulsifiers include, but are not limited to, gum arabic, sodium alkyl benzene sulfonate, sodium stearoyl lactylate, and the like.
Such thickeners include, but are not limited to, methylcellulose, carboxymethylcellulose, hydroxypropylcellulose, agar, sodium alginate, gelatin, and the like.
Such preservatives include, but are not limited to, potassium sorbate, sodium benzoate, parabens, and the like.
Advantageous effects
1. The extraction process of the beautyberry plant extract is simple, and the used raw material beautyberry plant has wide sources, long use history, economy and applicability and high safety;
2. the beautyberry plant extract according to the present invention is effective in scavenging free radicals, effectively inhibiting tyrosinase activity, effectively reducing ultraviolet absorption, and effectively combating skin aging.
Examples
The invention is illustrated below by means of examples, which do not limit the invention in any way.
In the following examples, the production areas of the callicarpa chinensis and the crocus sativus are Hubei, the production areas of the callicarpa kwangtungensis and the callicarpa macrophylla are Guangdong, and the dried leaves and stems of the respective herbs are pulverized into coarsest powder (the definition thereof is shown in the second part of the pharmacopoeia 2010). The flash extractor used for extraction is JHBE-50 type and is purchased from the ancient cooking science and technology development company of Henan golden tripod. The D101, HPD100 and AS-7 macroporous adsorption resins are all purchased from Hebei cang Baoyin chemical Co. Polyamide resin (80-120 mesh) was purchased from Chemicals, Inc., national drug group.
Unless otherwise indicated, the ethanol solution in the following examples refers to an ethanol aqueous solution, and is in volume percent.
Unless otherwise stated, the term "concentrate volume is equivalent to the amount of medicinal material" in the following examples means that the amount of the concentrate (in ml) is equal to the amount of the original medicinal material (in g); the bed volume of the resin column is 2-0.5 times of the volume of the supernatant to be treated.
In the following examples, the centrifugation conditions were 5000rpm, about 10 minutes.
Example 1
Pulverizing dried leaves of Callicarpa cathayana H.T. Chang into coarse powder, and percolating with 90% ethanol at flow rate of 1mL/h per gram of dry leaf coarse powder of Callicarpa cathayana for 10 h. Collecting percolate, and concentrating under reduced pressure to remove ethanol, wherein the volume of the concentrate is equal to the amount of the medicinal materials. Adding one time volume of water, standing overnight, and centrifuging to obtain supernatant. Passing the supernatant through D101 macroporous adsorbent resin column, washing with 6 times of resin column bed volume of water to remove impurities, eluting with 3 times of resin column bed volume of 40% ethanol, and collecting eluate. The eluate was concentrated under reduced pressure to remove ethanol. Diluting the concentrated solution to the volume of the initial supernatant with water, then passing through a polyamide chromatographic column, washing with water in an amount which is 2 times of the volume of the resin column bed, discarding the water washing solution, then eluting with 40% ethanol in an amount which is 3 times of the volume of the resin column bed, collecting the eluent, concentrating the eluent under reduced pressure, and drying to obtain the callicarpa leaf extract which is brown uniform powder.
Example 2
Pulverizing dried stems and leaves of Callicarpa cathayana H.T. Chang to coarse powder, adding 12 times (12 ml/g) of 80% ethanol, performing flash extraction for 3min for 2 times, mixing extractive solutions, and concentrating under reduced pressure to remove ethanol, wherein the volume of the concentrated solution is equal to the amount of the medicinal materials. Adding one time volume of water, standing overnight, and centrifuging to obtain supernatant. Passing the supernatant through D101 macroporous adsorbent resin column, washing with 6 times of resin column bed volume of water, discarding water washing solution, eluting with 3 times of resin column bed volume of 30% ethanol, and collecting eluate. Concentrating the eluate under reduced pressure, and removing ethanol. Diluting the concentrated solution to the volume of the initial supernatant with water, then passing through a polyamide chromatographic column, washing with water in an amount which is 2 times of the volume of the resin column bed, discarding the water washing solution, then eluting with 30% ethanol in an amount which is 3 times of the volume of the resin column bed, collecting the eluent, concentrating the eluent under reduced pressure, and drying to obtain the callicarpa bodinieri stem and leaf extract which is brown uniform powder.
Example 3
Pulverizing dried leaves of Durio grandiflora (Callicarpa formosana Rolfe) to coarse powder, adding 12 times (12 ml/g) of 70% ethanol, and performing flash extraction for 3min for 2 times. Mixing the two extracts, and concentrating the extractive solution under reduced pressure to remove ethanol, wherein the volume of the concentrated solution is equal to the amount of the medicinal materials. Adding one time volume of water, standing overnight, and centrifuging to obtain supernatant. Passing the supernatant through D101 macroporous adsorbent resin column, washing with 6 times of resin column bed volume of water, discarding water washing solution, eluting with 4 times of resin column bed volume of 30% ethanol, and collecting eluate. Concentrating the eluate under reduced pressure, and drying to obtain the extract of Rainbow leaf as brown uniform powder.
Example 4
Pulverizing dried stem and leaf of Durio grandiflora (Callicarpa fortmosana Rolfe) into coarse powder, adding 12 times (12 ml/g) of 70% ethanol, refluxing (heating to boiling under normal pressure) and decocting for 1 hr for 2 times. Mixing the two decoction extractive solutions, and concentrating under reduced pressure to remove ethanol, wherein the volume of the concentrated solution is equal to the amount of the medicinal materials. Adding one time volume of water, standing overnight, and centrifuging to obtain supernatant. Passing the supernatant through HPD100 macroporous adsorbent resin column, washing with 6 times of resin column bed volume of water, discarding water washing solution, eluting with 3 times of resin column bed volume of 40% ethanol, and collecting eluate. The eluate was concentrated under reduced pressure to remove ethanol. Diluting the concentrated solution with water to the volume of the initial supernatant, passing through ADS-7 resin column, washing with 3 times of resin column bed volume of water, discarding water washing solution, eluting with 3 times of resin column bed volume of 50% ethanol, and collecting eluate. Concentrating the eluate under reduced pressure, and drying to obtain extract of stem and leaf of Erythrophloe odorata as brown uniform powder.
Example 5
Pulverizing dried stem and leaf of Callicarpa kwangtungensis Chun into coarse powder, and percolating with 90% ethanol at flow rate of 1 mL/hr per gram of dry stem and leaf powder for 10 hr. Collecting percolate, and concentrating under reduced pressure to remove ethanol, wherein the volume of the concentrate is equal to the amount of the medicinal materials. Adding one time volume of water, standing overnight, and centrifuging to obtain supernatant. Passing the supernatant through D101 macroporous adsorbent resin column, washing with 6 times of resin column bed volume of water to remove impurities, eluting with 3 times of resin column bed volume of 70% ethanol, and collecting eluate. Concentrating the eluate under reduced pressure, and drying to obtain Callicarpa kwangtungensis Chun stem and leaf extract as brown uniform powder.
Example 6
Pulverizing dried stem of Callicarpa macrophylla Vahl to coarse powder, adding 10 times (10 ml/g dried stem powder of Callicarpa macrophylla) of 90% ethanol, refluxing (heating to boiling under normal pressure) and decocting for 1 hr for 2 times. Mixing the two decoction extractive solutions, and concentrating under reduced pressure to remove ethanol, wherein the volume of the concentrated solution is equal to the amount of the medicinal materials. Adding one time volume of water, standing overnight, and centrifuging to obtain supernatant. Passing the supernatant through D101 macroporous adsorbent resin column, washing with 3 times of resin column bed volume of water, discarding water washing solution, eluting with 3 times of resin column bed volume of 80% ethanol, and collecting eluate. Concentrating the eluate under reduced pressure, and drying to obtain Callicarpa macrophylla stem extract which is brown uniform powder.
Example 7
The scavenging ability of the beautyberry plant extract on free radicals (hydroxyl free radical, DPPH free radical and superoxide anion) is researched.
1 instruments and reagents
1.1 instruments
A constant temperature water bath, model HH-S11-2, purchased from Changan scientific instruments of Beijing; an ultraviolet visible spectrophotometer model TU-1800PC/TU-1800SPC was purchased from Beijing general analysis general instruments, Inc.;
1.2 reagents
1, 1 diphenyl-2-trinitrophenylhydrazine (DPPH) available from sigma; salicylic acid, available from national pharmaceutical group chemical agents, ltd; pyrogallol, available from national pharmaceutical group chemical reagents, ltd; hydrogen peroxide, purchased from national pharmaceutical group chemical reagents ltd; FeSO4From chemical reagents of the national drug group, ltd; 0.05mol/L Tris-HCl buffer, pH8.2, available from research area (Shanghai) Chemicals, Inc.; the other reagents are domestic chemical pure reagents.
1.3 samples
Beautyberry leaf extract, iridescent leaf extract, beautyberry stem and leaf extract and beautyberry stem extract were prepared as in examples 1, 3, 5 and 6, respectively; vitamin C, available from Bailingwei technologies, Inc.
2 method of experiment
2.1 hydroxyl radical scavenging test
Sequentially preparing 9mmol/L FeSO4、8.8mmol/L H2O2And 9mmol/L salicylic acid.
Preparation of a test sample solution: sequentially preparing the callicarpa chinensis leaf extract, the iridescent flower leaf extract, the callicarpa kwangtungensis stem and leaf extract, the callicarpa mauritiana stem extract and the vitamin C aqueous solution with the concentrations of 0.1mg/mL, 0.2mg/mL, 0.4mg/mL, 0.8mg/mL and 1.0 mg/mL.
Sequentially adding 2mL of FeSO into the colorimetric tube42mL salicylic acid, 2mL aqueous solution and 2mLH2O2Shaking up; heating in 37 deg.C water bath for 30min, taking out, and measuring absorbance A at 510nm0(ii) a Sequentially adding 2mL of FeSO into the colorimetric tube42mL salicylic acid, 2mL test samples at different concentrations, and 2mL H2O2Shaking up; heating in 37 deg.C water bath for 30min, taking out, and measuring absorbance A at 510nm1(ii) a Sequentially adding 2mL of FeSO into the colorimetric tube42mL of salicylic acid, 2mL of test samples with different concentrations and 2mL of aqueous solution, shaking up, heating in a water bath at 37 ℃ for 30min, taking out, and measuring the absorbance A at 510nm2(ii) a The removal rate of. OH by the test sample was calculated according to the following formula:
OH clearance (%) ═ 100 × (a)0-(A1-A2）)/A0
The hydroxyl radical scavenging test results are shown in figure 1. The higher the clearance rate, the stronger the oxidation resistance.
2.2DPPH free radical scavenging test
Accurately weighing 7.88mg of DPPH, dissolving with absolute ethyl alcohol, and fixing the volume in a 100mL volumetric flask, wherein the DPPH concentration is 2 multiplied by 10-4mo1/L, and storing in dark (0-4 ℃).
Preparation of a test sample solution: sequentially preparing the callicarpa chinensis leaf extract, the iridescent flower leaf extract, the callicarpa kwangtungensis stem and leaf extract, the callicarpa mauritiana stem extract and the vitamin C aqueous solution with the concentrations of 0.1mg/mL, 0.2mg/mL, 0.4mg/mL, 0.8mg/mL and 1.0 mg/mL.
Respectively and uniformly mixing 2mL of DPPH ethanol solution with 2mL of aqueous solution, 2mL of test sample aqueous solution with different concentrations with 2mL of DPPH ethanol solution, and 2mL of test sample aqueous solution with different concentrations with 2mL of aqueous solution, standing in the dark for 30min, measuring absorbances Ai, Aj and Ac of the test sample at 525nm by taking 50% ethanol as a blank, and calculating the DPPH-free radical clearance rate of the test sample according to the following formula:
the clearance (%) [1- (Ai-Aj)/Ac ] x 100%
In the formula: ac: absorbance of 2mL of the PPH ethanol solution +2mL of the aqueous solution; ai: absorbance of 2mL of test sample aqueous solution +2mL of ldpph ethanol solution; aj: absorbance of 2mL of aqueous test sample solution +2mL of aqueous solution.
The results of DPPH free radical scavenging test are shown in FIG. 2, where higher clearance is more potent in antioxidant capacity.
2.3 superoxide anion scavenging test
Preparing a reagent: 0.05mol/L Tris-HCl buffer solution with pH8.2; 10nmol/L HCL solution; 8mol/L HCL solution; 3mmol/L pyrogallol-10 mmol/L LHCL solution (3 mmol/L pyrogallol solution prepared with 10mmol/L HCL)
Preparation of a test sample solution: sequentially preparing the callicarpa chinensis leaf extract, the iridescent flower leaf extract, the callicarpa kwangtungensis stem and leaf extract, the callicarpa mauritiana stem extract and the vitamin C aqueous solution with the concentrations of 0.1mg/mL, 0.2mg/mL, 0.4mg/mL, 0.8mg/mL and 1.0 mg/mL.
Putting 4.5mL of Tris-HCl buffer solution into a test tube, and putting the test tube in a water bath at 25 ℃ for preheating; adding 4.2mL of aqueous solution, placing in a water bath at 25 deg.C, keeping the temperature for 20min, taking out, immediately adding 0.3mL of pyrogallol solution with concentration of 3mmol/L (preheating in a water bath at 25 deg.C), shaking, rapidly adding into a colorimetric tube, reacting for 5min, adding 1.0mL of HCL solution with concentration of 8mol/L to stop reaction, and measuring absorbance A at 325nm0。
Putting 4.5mL of Tris-HCl buffer solution into a test tube, and putting the test tube in a water bath at 25 ℃ for preheating; adding 4.2mL of test sample aqueous solution with different concentrations, placing in a water bath at 25 ℃ for heat preservation for 20min, taking out, immediately adding 0.3mL of pyrogallol solution with the concentration of 3mmol/L (preheating in the water bath at 25 ℃), shaking up, rapidly adding into a colorimetric tube, reacting for 5min, adding 1.0mL of HCL solution with the concentration of 8mol/L to stop the reaction, and measuring the absorbance A at 325nm1。
Putting 4.5mL of Tris-HCl buffer solution into a test tube, and putting the test tube in a water bath at 25 ℃ for preheating; adding 4.2mL of test sample aqueous solution with different concentrations, placing in a water bath at 25 deg.C, keeping the temperature for 20min, taking out, immediately adding 0.3mL of HCL solution with concentration of 3mmol/L and concentration of 10mmol/L (preheating in water bath at 25 deg.C), shaking, and rapidly adding into a colorimetric tubeAfter 5min of reaction, 1.0mL of 8mol/L HCl solution was added to terminate the reaction, and the absorbance A was measured at 325nm2。
Clearance (%) < 100 × (A)0-(A1-A2))/A0
In the formula: a. the0Absorbance of the aqueous solution + pyrogallol, A1The absorbance of the test sample water solution and the pyrogallol solution is measured; a. the2The absorbance of the aqueous sample solution +10mmol/LHCL solution was tested.
The superoxide anion scavenging test results are shown in figure 3. The higher the clearance rate, the stronger the oxidation resistance.
3 results of the test
The ability to scavenge hydroxyl radicals, DPPH radicals, superoxide anion radicals is a common method of evaluating antioxidant ability. The removing results of the callicarpa chinensis leaf extract, the iridescent flower leaf extract, the callicarpa kwangtungensis stem and leaf extract and the callicarpa kwangtungensis stem extract on hydroxyl free radicals, DPPH free radicals and superoxide anion free radicals are shown in figures 1-3. As shown in fig. 1-3, with increasing concentration, the radical scavenging rate of each beautyberry leaf extract and the positive control vitamin C increases, the radical scavenging capacity of the beautyberry leaf extract and the iridoid flower leaf extract is stronger than that of the positive control vitamin C, and the radical scavenging capacity of the beautyberry stem leaf extract and the beautyberry stem extract is equivalent to that of the positive control vitamin C. And (4) conclusion: the callicarpa chinensis leaf extract, the iridoid flower leaf extract, the callicarpa kwangtungensis stem and leaf extract and the callicarpa mauritiana stem extract all have strong oxidation resistance, and the callicarpa chinensis leaf extract has the strongest oxidation resistance.
Example 8
The invention relates to a research on the inhibition capacity of beautyberry plant extract on tyrosinase activity.
1 instruments and reagents
1.1 instruments
An ultraviolet visible spectrophotometer, model TU-1800PC/TU-1800SPC, available from Beijing general analytical instruments, Inc.; an electronic analytical balance, BT25S, available from sydows scientific instruments (beijing) ltd; DZF-6050 vacuum drying oven (Shanghai sperm macro test Equipment Co., Ltd.).
1.2 reagents
PBS buffer: preparing a buffer solution with pH of 6.85 according to pharmacopoeia; l-tyrosine is purchased from chemical reagents of national medicine group, and prepared into 0.1 percent aqueous solution; mushroom tyrosinase, purchased from sigma, USA, prepared into 0.05mg/mL aqueous solution; the water is deionized water; the other reagents are all domestic analytical purifiers.
1.3 samples
Beautyberry leaf extract, iridescent leaf extract, beautyberry stem and leaf extract and beautyberry stem extract were prepared as in examples 1, 3, 5 and 6, respectively; arbutin, available from Aladdin reagent (Shanghai) Co., Ltd.
2 method of experiment
Sucking 0.5mL L-tyrosinase aqueous solution and 2.0mL LPBS buffer solution, mixing, placing in 37 deg.C water bath at constant temperature for 30min, adding 0.5mL Agaricus campestris tyrosinase solution, mixing, reacting for 10min, and determining absorbance A at 475nm1。
Sucking 2.5mL PBS buffer solution, placing in 37 deg.C water bath, maintaining the temperature for 30min, adding 0.5mL mushroom tyrosinase solution, mixing, reacting for 10min, and determining absorbance A at 475nm2。
Sucking 0.5mL L-tyrosinase aqueous solution, 0.5mL test sample aqueous solution with different concentrations, and 1.5mL PBS buffer solution, mixing, placing in 37 deg.C water bath at constant temperature for 30min, adding 0.5mL mushroom tyrosinase solution, mixing, reacting for 10min, and determining absorbance A at 475nm3。
Sucking 0.5mL of test sample water solution with different concentrations and 2.0mL of LPBS buffer solution, mixing, placing in 37 deg.C water bath at constant temperature for 30min, adding 0.5mL of mushroom tyrosinase solution, mixing, reacting for 10min, and determining absorbance A at 475nm4。
The inhibition rate of the callicarpa extract on tyrosinase is calculated according to the following formula:
inhibition rate (%) ([ 1- (A) ]3-A4)/(A1-A2)]×100％
In the formula: a. the1: absorbance values of control solutions (L-tyrosine aqueous solution + mushroom tyrosinase aqueous solution + PBS buffer solution)
A2: absorbance values of control blank solution (mushroom tyrosinase aqueous solution + PBS buffer solution)
A3: absorbance values of test sample solutions (L-tyrosine aqueous solution + mushroom tyrosinase aqueous solution + test sample aqueous solution + PBS buffer solution)
A4: absorbance values of test sample blank solution (mushroom tyrosinase aqueous solution + test sample aqueous solution + PBS buffer solution)
The samples tested in this experiment were aqueous solutions of Callicarpa Huaihua leaf extract, Erythroseum leaf extract, Callicarpa kwangtungensis stem and leaf extract and Callicarpa mauritiana stem extract prepared according to examples 1, 3, 5 and 6, and the tested concentrations were 0.2mg/mL, 0.4mg/mL, 0.8mg/mL, 1.2mg/mL, 1.6mg/mL, 2.0mg/mL, 2.5mg/mL, 5.0mg/mL and 10.0mg/mL, respectively.
3 results of the test
The tyrosinase activity inhibition experiment is a common whitening and freckle removing capability evaluation method. The inhibition of tyrosinase activity by Callicarpa kwangtungensis leaf extract, Callicarpa pallidiflora leaf extract, Callicarpa kwangtungensis stem and leaf extract, Callicarpa mauritiana stem extract, and positive control arbutin is shown in FIG. 4. As shown in fig. 4, the inhibition rate of the tyrosinase activity by each callicarpa extract and the positive control arbutin increases with the increase of the concentration, wherein the tyrosinase activity inhibition ability of the callicarpa chinensis leaf extract and the erythrina indica leaf extract is stronger than that of the positive control arbutin, and the callicarpa kwangtungensis stem leaf extract and the callicarpa mauritiana stem extract are equivalent to that of the positive control arbutin. And (4) conclusion: the callicarpa extract has strong tyrosinase activity inhibition effect, and can achieve the effects of removing freckles and whitening by inhibiting the tyrosinase activity.
Example 9
The ultraviolet absorption capacity of the beautyberry plant extract is researched.
1 instruments and reagents
1.1 instruments
An ultraviolet visible spectrophotometer, model TU-1800PC/TU-1800SPC, available from Beijing general analytical instruments, Inc.; an electronic analytical balance, BT25S, available from sydows scientific instruments (beijing) ltd.
1.2 reagents
Ethanol, available from national pharmaceutical group chemical agents, ltd.
2 method of experiment
2.1 detection method
The test samples (Callicarpa kwangsiensis leaf extract, Erythroseum forbesii leaf extract, Callicarpa kwangtungensis stem and leaf extract and Callicarpa macrophylla stem extract prepared according to examples 1, 3, 5 and 6, respectively) were diluted with 50% ethanol aqueous solution to 0.5 mg/ml of crude drug, and scanned in a wavelength range of 200-400nm using 50% ethanol aqueous solution as a blank, and the light transmittance at each wavelength was measured, and the measurement wavelengths were divided into the following zones:
200, 210, 220, 230, 240, 250, 260, 270, 280nm of a UV-C region (short wave ultraviolet region, 200 and 280 nm);
280, 290, 300, 310, 320nm of a UV-B region (a medium wave ultraviolet region, 280-320 nm);
320, 330, 340, 350, 360, 370, 380, 390, 400nm of the UV-A region (long wave ultraviolet region, 320-400 nm).
2.2 evaluation method
Those with an ultraviolet absorptivity of 80% or more are considered to have a sunscreen effect; when the UV absorption is more than 90%, the sunscreen effect is considered to be strong.
3 results of the test
The results of the UV absorbance of the test samples at different wavelengths are shown in Table 1 below:
TABLE 1 UV-C, UV-B, UV-A UV absorptivity of extracts of beautyberry plants
Ultraviolet absorption ability is a commonly used evaluation method of sun protection ability. The results show that the ultraviolet absorption capacity of the callicarpa chinensis extract and the iridescent extract to different wavelengths exceeds 90 percent, and the ultraviolet absorption capacity of the callicarpa kwangtungensis extract and the callicarpa macrolobata extract to different wavelengths also exceeds 80 percent. And (4) conclusion: the beautyberry plant extract can effectively reduce the absorption of ultraviolet rays, and is an ideal broad-spectrum sunscreen agent.
Example 10
The beautyberry plant extract of the invention is used for researching the effect of resisting skin aging.
1 instruments and reagents
1.1 instruments
Homogenizer, model JYT-10, available from Shanghai FLUKO.
1.2 reagents
D-galactose purchased from Shanghai Kogyi II; sodium sulfide barbituric acid, available from Sigma; ethanol and glycerol, available from chemical reagents of national drug group, ltd; polyoxyethylene (2) stearyl ether, polyoxyethylene (21) stearyl ether, chemical grade, available from prochloraz chemicals, ltd; isooctyl palmitate, dimethicone, jojoba oil, cetostearyl alcohol, glyceryl monostearate, methylparaben, chemical grade, available from Shanghai Lisheng, Inc.; SOD kit, HYP kit and MDA kit are purchased from Nanjing Biotechnology engineering company.
1.3 samples
The callicarpa chinensis leaf extract was prepared as in example 1.
1.4 animals
3-month-old clean-grade Kunming female nude mice (Experimental animals center of Suzhou university college of medicine), the average weight of the nude mice is 30 +/-2 g, and the nude mice are fed with standard feed.
2 method of experiment
2.1 preparation of test cosmetics
The formulations of the test cosmetics are shown in table 2 below.
TABLE 2 Callicarpa kwangsiensis leaf extract cosmetic formulations
| Raw materials | Composition (mass%) |
| Callicarpa kwangtungensis leaf extract | 2.0 |
| Polyoxyethylene (2) stearyl alcohol ether | 2.5 |
| Polyoxyethylene (21) stearyl alcohol ether | 1.5 |
| Jojoba oil | 4.0 |
| Cetostearyl alcohol | 3.0 |
| Glyceryl monostearate | 2.5 |
| Stearic acid | 0.2 |
| Palmitic acid isooctyl ester | 0.2 |
| Ethanol | 0.5 |
| Nipagin methyl ester | 0.5 |
| Water (W) | Balance of |
Mixing polyoxyethylene (2) stearyl alcohol ether, polyoxyethylene (21) stearyl alcohol ether, jojoba oil, cetearyl alcohol, glyceryl monostearate, and stearic acid, heating to 90 deg.C, stirring, and keeping the temperature as phase A; dissolving isooctyl palmitate in deionized water, heating to 90 ℃ to serve as a B phase, and preserving heat for later use; dissolving folium Callicarpae Formosanae extract in water and appropriate amount of ethanol to obtain phase C; slowly adding the phase B into the phase A under stirring of a homogenizer when the temperatures of the two phases are approximately the same, and continuously homogenizing for about 5min by the homogenizer; and then, controlling the temperature to be about 75 ℃, preserving the heat, stirring and defoaming, adding the phase C when the temperature of the system is reduced to 35 ℃, simultaneously adding the methyl paraben, continuously and slowly stirring, discharging at room temperature, and filling into a sterilized bottle to obtain the product.
2.2 animal grouping, administration and modeling
30 nude mice were randomly divided into 3 groups of 10 mice each. Feeding the blank control group under normal conditions without injection and illumination treatment; model group was injected subcutaneously (sc) with D-galactose 1000 mg/kg. D in the neck and back per day, while removing back hair and part of horny layer with 15% sodium sulfide paste, exposing skin of about 3cm × 5cm, and irradiating back skin with ultraviolet rays of long wavelength (365nm) and short wavelength (254nm) with cumulative irradiation intensity of 259.5J/cm2The cumulative irradiation intensity of the short wavelength is 6.482J/cm2. The test cosmetic was applied to the back of the test group 2 times daily at a dose of 0.2 g/time on the basis of injection and light irradiation.
After the model group is subjected to continuous injection of D-galactose and illumination for 6 weeks, the injection of D-galactose and illumination are stopped, the back wrinkles are obvious, and obvious aging signs are displayed, and all animals are sacrificed after 2 days.
2.3 preparation of skin homogenate (10%)
Taking about 1g of a nude mouse back skin tissue block, rinsing with precooled normal saline, removing subcutaneous fat and other connective tissues, wiping dry with filter paper, weighing, measuring 9 times weight of normal saline of the tissue with a graduated cylinder, pouring 2/3 into a mortar filled with the tissue block, cutting with small ophthalmic scissors, grinding for 15min, pouring a coarse homogenate into a small beaker, flushing the mortar with the rest 1/3 of normal saline, continuously crushing for 3min in an ultrasonic crusher (with the strength of 25%) in an ice bath, repeatedly freezing and thawing for 3 times to completely free cell contents in a liquid phase, centrifuging the tissue homogenate for 15min at 3500r/min, and taking a supernatant.
2.4 determination of SOD Activity, HYP content and MDA content in skin tissue homogenate
SOD activity, HYP content and MDA content in skin tissue homogenate are measured by referring to the method of kit instructions of SOD (superoxide dismutase), HYP (hydroxyproline) and MDA (malondialdehyde) of Nanjing Biotechnology engineering company.
3 results of the experiment
The results of the biochemical measurements in the skin tissues of nude mice are shown in Table 3 below:
TABLE 3 measurement results of biochemical indices in skin tissues of nude mice
n＝10)
| Group of | SOD(U/mL) | HYP(μg/mL) | MDA(nmol/mL) |
| Blank control group | 120.59±6.20＊＊ | 1.49±0.18＊＊ | 15.42±4.96＊＊ |
| Model set | 81.23±4.15 | 0.88±0.18 | 55.48±6.89 |
| Experimental group | 118.13±5.78＊＊ | 1.21±0.29＊& | 12.42±3.41＊＊& |
Note that ＊ p is less than 0.05 and ＊＊ p is less than 0.01 when compared with the model group, and when compared with the blank control group,&p＜0.05。
HYP content, SOD activity and MDA (malondialdehyde) content are common evaluation indexes of anti-aging capability. The result shows that the beautyberry extract can improve the HYP content and SOD activity in the skin tissue of a subacute aging and photoaging model nude mouse, and can reduce the MDA content. And (4) conclusion: the folium Callicarpae Formosanae extract has skin aging resisting effect.
The foregoing embodiments and examples of the present invention have been described for the purposes of illustration and description only and are not intended to limit the invention in any way. It is evident that many modifications and variations will be apparent to those skilled in the art in light of the teachings of this invention. Such modifications and variations are within the spirit and scope of the invention as defined by the appended claims.
Claims (7)
1. Use of an extract of a plant of the genus Callicarpa as a tyrosinase inhibitor and/or ultraviolet absorber in the preparation of a cosmetic, wherein the extract of the genus Callicarpa is prepared from the stems and/or leaves of a plant of the genus Callicarpa of the family Verbenaceae selected from Callicarpa chinensis (Callicarpa cathayana H.T. Chang), Callicarpa formosana Rolfe, Callicarpa macrophylla Vahl, and Callicarpa kwangtungensis Chun by an alcohol extraction method or a water-alcohol mixed extraction method.
2. Use of an extract of a plant of the genus Callicarpa as a superoxide anion radical scavenger in the preparation of cosmetics, wherein the extract of the genus Callicarpa is prepared from the stems and/or leaves of a plant of the genus Callicarpa of the family Verbenaceae selected from Callicarpa chinensis (Callicarcacathayana H.T. Chang), Callicarpa macrophylla Vahl (Callicarpa macrocarpa) and Callicarpa kwangtungensis Chun (Callicarpa kwangtungensis Chun) by an alcohol extraction method or a water-alcohol mixed extraction method.
3. Use according to claim 1 or 2, wherein the cosmetic comprises 0.1-10.0% by weight of beautyberry plant extract.
4. The use according to claim 1 or 2, wherein the beautyberry plant extract is prepared by a process comprising the steps of:
(1) crushing stems and/or leaves of beautyberry plants, and extracting for 1-3 times by using a solvent, wherein the solvent is ethanol or a mixture of water and ethanol;
(2) mixing the extractive solutions, and concentrating under reduced pressure to remove the solvent in step (1); adding water with the volume 0.5 to 2 times that of the mixture, standing overnight, and centrifuging or filtering to obtain supernatant;
(3) passing the supernatant through a chromatographic column filled with resin filler, washing with water to remove impurities, eluting with 30-90 vol% aqueous alcohol, collecting the eluate, concentrating under reduced pressure, and drying to obtain the Callicarpa plant extract.
5. The use according to claim 4, wherein in step (1), the extraction method is selected from the group consisting of flash extraction, reflux extraction, microwave extraction, ultrasonic extraction and percolation extraction.
6. Use according to claim 4, wherein in step (3) the resin filler is selected from macroporous adsorption resins, polyamide resins and ion exchange resins.
7. Use according to claim 1 or 2, wherein the cosmetic product is a cream, milk, liquid, powder or spray.
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